Fluid system of a fluorescent immunoassay analyzer

Abstract

The present invention provides a fluid path system of a fluorescent immunoassay analyzer, and relates to the field of blood analysis. The present invention includes an immunoassay analyzer structure, the immunoassay analyzer structure includes a base and a square slot, the immunoassay analyzer structure is installed with a fluid path system and a sample support structure, the square slot is formed on the top of the base, the fluid path system is partially set to the top of the base through the square slot, the sample support structure is installed on the top of the base, when the fluid path system supplies diluent to the immunoassay analyzer structure, it is ensured as much as possible that the diluent used by the immunoassay analyzer structure is clean each time, and the pipe fittings of the temporarily stored diluent are cleaned to ensure the accuracy of the fluorescence analysis of the blood sample, and quantitative diluent is supplied multiple times according to the number of multiple blood samples to reduce the waste of diluent.

Description

Technical Field

[0001] The invention relates to a liquid circuit system, in particular to a liquid circuit system of a fluorescent immunoassay analyzer, belonging to the technical field of blood analysis. Background Art

[0002] As a commonly used technical means in biomedical testing, fluorescent immunoassay technology has a series of advantages such as high sensitivity, strong specificity, fast detection speed, safety and stability. Fluorescent immunoassay technology can be used to measure very low levels of biologically active compounds, such as proteins (enzymes, receptors, antibodies), hormones (steroids, thyroid hormones, phthalosteroid hormones), drugs and microorganisms. Currently, fluorescent immunoassay technology has been widely used in infectious disease detection, tumor marker detection, blood and cytology detection and other detection fields.

[0003] For example, the invention disclosed in the patent application number: 202210241111.6 discloses a disc-type microfluidic fluorescent immunoassay analyzer and its use method; the analyzer main body includes a reagent chamber assembly, a Tip head assembly, and a sample assembly; the reagent chamber assembly includes a reagent chamber and a reagent reagent kit arranged in the reagent chamber; the Tip head assembly includes a Tip head and a diluent bottle; the sample assembly includes a test tube rack and a test tube placed on the test tube rack; the robotic arm is electrically connected to the electronic control system; the robotic arm can move to the test tube and uncap the test tube; the robotic arm can load the Tip head and drive the Tip head to move to the diluent bottle to absorb the diluent in the diluent bottle.

[0004] The whole process of the above-mentioned fluorescent immunoassay analysis involves a dilution step, and it is necessary to manually check whether there is enough diluent in the diluent bottle. Subjective judgment can easily lead to insufficient diluent during the analysis and detection process, which requires manual addition. In addition, the diluent is in an exposed state during the detection process and is easily contaminated, affecting the quality of sample analysis and detection. Summary of the invention

[0005] To solve the above problems, the present invention is implemented through the following technical solutions: a fluid path system of a fluorescent immunoassay analyzer, comprising an immunoassay analyzer structure, the immunoassay analyzer structure comprising a base and a square groove, a fluid path system and a sample support structure are installed on the immunoassay analyzer structure, the square groove is formed on the top of the base, the fluid path system part is arranged to the top of the base through the square groove, and the sample support structure is installed on the top of the base;

[0006] The fluid circuit system comprises a forward and reverse motor and a sliding member, a rotating frame is installed at the output end of the forward and reverse motor, a solenoid valve is fixedly installed inside the rotating frame, the input end of the solenoid valve is connected to a liquid storage tank, the output end of the solenoid valve is fixedly connected to a pipe fitting, and an outer ring groove is provided on the outer side of the pipe fitting;

[0007] The inner recess of the sliding member 1 is slidably connected with a sliding member 2, and both sides of the sliding member 2 are fixedly connected with a mounting frame 1, and an electromagnet is provided on a side of the mounting frame 1 close to the sliding member 1, and the electromagnet is fixedly connected to the sliding member 1, and the two mounting frames 1 are fixedly connected with a pneumatic cylinder 1, and a pipe fitting 5 is fixedly installed between the two pneumatic cylinders 1, and a stirring member is rotatably connected inside the pipe fitting 5; the stirring member is fixedly connected with two solenoid valves 2, and the stirring member is fixedly connected with a guide box, and a rotating ring is rotatably connected inside the guide box, and the rotating ring is fixedly connected with a telescopic tube 1, and the telescopic tube 1 is fixedly connected with a pipe fitting 6; the stirring member is fixedly connected with a forward and reverse motor 2, and a sliding connection component is provided between the forward and reverse motor 2 and the sliding member 2;

[0008] The fluid circuit system comprises two pipe fittings 7, a mounting frame 6 is fixedly connected between the two pipe fittings 7, and a pneumatic cylinder 3 is fixedly connected between the mounting frame 6 and a sliding member 1.

[0009] Preferably, the immunoassay analyzer structure includes a centrifugal module, a reagent chamber, a Tip head assembly and a casing, the casing is installed on one side of the top of the base and fixedly connected to the liquid storage tank, and the centrifugal module, the reagent chamber, and the Tip head assembly are all installed on the top of the base.

[0010] Preferably, the sample support structure comprises a U-shaped support frame, a pressure sensor is arranged inside the U-shaped support frame, a plurality of elastic members are fixedly connected inside the U-shaped support frame, and a pad is fixedly connected to the bottom of the elastic member.

[0011] Preferably, the sample support structure includes a clamping frame, and a plurality of mounting bent rods are fixedly connected to the base on both sides of the clamping frame, and a plurality of sample placement grooves are provided on the top of the clamping frame and the top of the U-shaped support frame, and the plurality of sample placement grooves provided on the top of the U-shaped support frame correspond one-to-one to a plurality of elastic members.

[0012] Preferably, the clamping frame is fixedly connected to a three-way valve, the three-way valve is fixedly connected to an air pipe, both sides of the air pipe are fixedly connected to a plurality of clamping members, and the normally open end of the three-way valve is fixedly connected to an air pump.

[0013] Preferably, the forward and reverse motor 1 is fixedly connected to the base, a semicircular plate is fixedly connected to the outer side of the pipe fitting 5, a fixing ring is fixedly connected to the outer top of the pipe fitting 5, the outer wall of the pneumatic cylinder 1 passes through the fixing ring, the piston end of the pneumatic cylinder 1 is fixedly connected to a mounting frame 2, the mounting frame 2 is fixedly connected to the pipe fitting 5, the input end of the solenoid valve 1 is rotatably connected to the pipe fitting 2, one end of the pipe fitting 2 is fixedly connected to a flow controller, and the flow controller is fixedly connected to the liquid storage tank.

[0014] Preferably, the sliding connection assembly includes a pipe fitting three, the pipe fitting three is fixedly connected to the sliding member two, a pipe fitting four is arranged inside the pipe fitting three, the pipe fitting four is fixedly sleeved on the outside of the forward and reverse motor two, a telescopic rod one is fixedly connected between the forward and reverse motor two and the sliding member two, and the telescopic rod one is arranged inside the pipe fitting four.

[0015] Preferably, the electromagnet is fixedly connected to a sliding member, a telescopic rod 2 is fixedly connected between the mounting frame 1 and the rotating ring, the pipe fitting 6 passes through the mounting frame 1 and is fixedly connected to the mounting frame 1, the solenoid valve 2 is connected to the interior of the guide box, an inner ring groove is provided inside the pipe fitting 5, a rubber pad 1 is provided inside the inner ring groove, and the rubber pad 1 is fixedly connected to the pipe fitting 5.

[0016] Preferably, two circular holes are provided on the top of the sliding member 1, the top ends of the two pipe fittings 7 extend to the inside of the two circular holes respectively, a rubber pad 2 is fixedly connected inside the pipe fitting 7, a hose is fixedly connected to the bottom of the two pipe fittings 7, a water pump is fixedly connected to the bottom ends of the two hoses, a pipe fitting 8 is fixedly connected to one side of the water pump, a mounting frame 7 is fixedly connected to the base, and the pipe fitting 8 passes through the mounting frame 7 and is fixedly connected to the mounting frame 7.

[0017] Preferably, a mounting frame three is fixedly connected to the bottom of the base, an operating bolt is threadedly connected to the mounting frame three, one end of the operating bolt is rotatably connected to a clamping plate, the mounting frame three is slidably connected to multiple mounting frames four, multiple mounting frames four are all fixedly connected to a sliding member one, a mounting frame five is fixedly connected between the mounting frame three and the two water pumps, the end of the sliding member one away from the mounting frame three is fixedly connected to a guide rail, a guide ball is arranged inside the guide rail, one end of the guide ball is rotatably connected to a pneumatic cylinder two, and the pneumatic cylinder two is fixedly connected to the mounting frame seven.

[0018] The present invention provides a fluid path system of a fluorescent immunoassay analyzer, which has the following beneficial effects:

[0019] 1. The liquid circuit system of the fluorescence immunoassay analyzer, when supplying diluent to the immunoassay analyzer structure, tries to ensure that the diluent used by the immunoassay analyzer structure each time is clean, and cleans the pipes for temporarily storing the diluent, to ensure the accuracy of the fluorescence analysis of the blood sample, and supplies a fixed amount of diluent multiple times according to the number of blood samples, to reduce the waste of diluent, to avoid the situation where the diluent is used after being in contact with the air for a long time, and to reduce the situation where the blood sample analysis is inaccurate due to the quality change of the diluent.

[0020] 2. The liquid circuit system of the fluorescent immunoassay analyzer can supply a corresponding amount of diluent to the inside of the tube 1 according to the weight of blood in the blood sample container to be analyzed by controlling the working time of the solenoid valve 1, and automatically ensure sufficient supply of diluent, so as to avoid the situation that the staff manually checks the amount of diluent, resulting in insufficient diluent during the analysis of multiple blood samples, or a large amount of diluent that has not been sealed for a long time is destroyed after the blood sample analysis is completed, thereby reducing the waste of diluent. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the structure of installing a bent rod according to the present invention;

[0023] Figure 3 It is a structural schematic diagram of the sample support structure of the present invention;

[0024] Figure 4 It is a schematic diagram of the structure of the airway tube of the present invention;

[0025] Figure 5 It is a structural schematic diagram of the base of the present invention;

[0026] Figure 6 It is a structural schematic diagram of the mounting frame 7 of the present invention;

[0027] Figure 7 For the present invention Figure 6 A schematic diagram of the structure of part A;

[0028] Figure 8 It is a structural schematic diagram of the liquid circuit system of the present invention;

[0029] Fig. 9 It is a schematic diagram of the structure of the hose of the present invention;

[0030] Fig.10 It is a structural schematic diagram of a sliding member 1 of the present invention;

[0031] Fig.11 For the present invention Fig.10 Schematic diagram of the C part structure;

[0032] Fig.12 It is a structural schematic diagram of a mounting frame 1 of the present invention;

[0033] Fig.13 It is a structural schematic diagram of the fixing ring of the present invention;

[0034] Fig.14 It is a schematic diagram of the local structure of the pipe fitting 5 of the present invention.

[0035] Description of the reference numerals: 1. immunoassay analyzer structure; 11. base; 12. centrifugal module; 13. reagent compartment; 14. tip head assembly; 15. square groove; 16. housing; 2. sample support structure; 21. U-shaped support frame; 22. pressure sensor; 23. mounting bent rod; 24. clamping frame; 25. sample placement slot; 26. clamping member; 27. air guide tube; 28. three-way valve; 29. ​​air pump; 210. elastic member; 211. pad; 3. liquid circuit system; 31. forward and reverse motor 1; 32. rotating frame; 33. solenoid valve 1; 34. pipe fitting 1; 35. outer ring groove; 36. pipe fitting 2; 37. flow controller; 38. liquid storage tank; 39. sliding member 1; 310. sliding member 2; 311. mounting frame 1; 312. electromagnet; 313. pipe fitting 3; 314. telescopic rod 1; 31 5. Forward and reverse motor 2; 316. Pipe fitting 4; 317. Agitator; 318. Pipe fitting 5; 319. Inner ring groove; 320. Rubber pad 1; 321. Fixed ring; 322. Air cylinder 1; 323. Mounting frame 2; 324. Guide box; 325. Rotating ring; 326. Telescopic tube 1; 327. Pipe fitting 6; 328. Telescopic rod 2; 329. Semicircular plate; 330. Mounting frame 3; 331 , operating bolt; 332, clamping plate; 333, mounting frame four; 334, mounting frame five; 335, water pump; 336, hose; 337, pipe fitting seven; 338, rubber pad two; 339, round hole; 340, guide rail; 341, guide ball; 342, pneumatic cylinder two; 343, mounting frame six; 344, pneumatic cylinder three; 345, pipe fitting eight; 346, mounting frame seven; 347, solenoid valve two. DETAILED DESCRIPTION

[0036] An embodiment of the present invention provides a fluid path system of a fluorescent immunoassay analyzer.

[0037] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 , Fig. 9 , Fig.10 , Fig.11 , Fig.12 , Fig.13 and Fig.14 , including an immunoassay analyzer structure 1, which includes a base 11 and a square groove 15, on which a liquid path system 3 and a sample support structure 2 are installed, the square groove 15 is formed on the top of the base 11, and part of the liquid path system 3 is set to the top of the base 11 through the square groove 15, and the sample support structure 2 is installed on the top of the base 11.

[0038] Specifically, in the present application, the casing 16 is used to install a robotic arm, and the immunoassay analyzer structure 1 is composed of a centrifugal module 12, a reagent compartment 13, a Tip head assembly 14, a casing 16, and a robotic arm installed on the top of the casing 16 (for details, please refer to the invention of patent application number: 202210241111.6, which discloses a disc-type microfluidic fluorescence immunoassay analyzer and its use method), and a human-computer interaction device is used to control the operation of the immunoassay analyzer structure 1 (this is a public technology application). The casing 16 is installed on one side of the top of the base 11 and is fixedly installed with a liquid storage tank 38 for storing the diluent.

[0039] A sample support structure 2 for placing a blood sample container is installed on the top of the immunoassay analyzer structure 1. A pressure sensor 22 is arranged inside a U-shaped support frame 21 in the sample support structure 2. A plurality of elastic members 210 fixedly connected inside the U-shaped support frame 21 are fixedly connected to a pad 211 at the bottom. Figure 3 As shown, a plurality of pads 211 are arranged on the top of the pressure sensor 22, and a plurality of mounting bent rods 23 are fixedly connected between the base 11 and both sides of the clamping frame 24 in the sample support structure 2. The clamping frame 24 is arranged on the top of the U-shaped support frame 21 under the support of the plurality of mounting bent rods 23. A plurality of sample placement grooves 25 are provided on the top of the clamping frame 24 and the top of the U-shaped support frame 21. The plurality of sample placement grooves 25 provided on the U-shaped support frame 21 correspond one-to-one to the plurality of sample placement grooves 25 provided on the clamping frame 24, and the plurality of sample placement grooves 25 provided on the top of the U-shaped support frame 21 correspond one-to-one to the plurality of elastic members 210. After the bottom end of the blood sample container passes through the sample placement grooves 25 provided on the clamping frame 24 and the sample placement grooves 25 provided on the U-shaped support frame 21 in sequence, the bottom end of the blood sample container falls on the pad 211, and the pad 211 is fixedly connected to the sample placement grooves 25 provided on the clamping frame 24 under the action of the blood sample container. The elastic member 210 is deformed and extended, and the pad 211 falls to the top of the pressure sensor 22. The weight value detected by the pressure sensor 22 changes, and multiple blood sample containers are placed in the sample support structure 2 in sequence according to the preset order. The pressure sensor 22 feeds back the weight data changes detected after each blood sample container is placed to the human-computer interaction device, and the human-computer interaction device can calculate and record the weight of each blood sample container. Since the blood sample containers are of a standard weight, the weight of the sample blood inside the blood sample container at each position can be easily calculated, and the elastic member 210 and the pad 211 are arranged between the blood sample containers, which satisfies the pressure sensor 22 in measuring the weight of the sample blood inside the blood sample container while preventing the blood sample in the blood sample container from dripping onto the top of the pressure sensor 22 and causing contamination to the pressure sensor 22.

[0040] After multiple blood sample containers are placed, the human-computer interaction device performs fluorescent immunoassay on the blood samples according to the control immunoassay analyzer structure 1 stored in advance inside. This is a public technology. For details of the steps, please refer to the invention disclosed in patent application number: 202210241111.6, which discloses a disc-type microfluidic fluorescent immunoassay analyzer and its use method.

[0041] The robot arm analyzes the blood samples in the multiple blood sample containers supported by the sample support structure 2 in sequence according to a preset order. When the robot arm clamps the Tip head to absorb the diluent, the human-computer interaction device controls the liquid path system 3 to work according to the weight of the blood sample to be analyzed, and provides diluent for the fluorescence analysis of the blood sample. The specific steps are as follows:

[0042] Step 1: The human-computer interaction device controls the electromagnetic valve 1 33 in the liquid circuit system 3 to work for a period of time according to the blood weight inside the blood sample container to be analyzed, and controls the flow controller 37 to work and open. The flow controller 37 controls the discharge speed of the diluent inside the liquid storage tank 38. After the electromagnetic valve 1 33 is turned on, since the input end of the electromagnetic valve 1 33 is rotatably connected to the pipe 2 36, the flow controller 37 fixedly connected to one end of the pipe 2 36 is connected to the inside of the liquid storage tank 38. Figure 6 As shown, the height of the liquid storage tank 38 is located above the electromagnetic valve 1 33; therefore, the diluent in the liquid storage tank 38 enters the pipe 1 34 fixedly connected to the top of the electromagnetic valve 1 33 through the flow controller 37, the pipe 2 36 and the electromagnetic valve 1 33 under the action of gravity. By controlling the working time of the electromagnetic valve 1 33, a corresponding amount of diluent can be supplied to the pipe 1 34 according to the weight of the blood in the blood sample container to be analyzed, and the diluent is automatically supplied in sufficient quantity, thereby avoiding the situation where the staff manually checks the amount of diluent, resulting in insufficient diluent during the analysis of multiple blood samples, or a large amount of diluent that has not been sealed for a long time is destroyed after the blood sample analysis is completed, thereby reducing the waste of diluent.

[0043] Step 2: The robot arm holds the Tip head to absorb the diluent from the inside of the pipe 34 and then leaves;

[0044] Step 3: The forward and reverse motor 31 fixedly installed at the bottom of the human-machine interaction device controls the base 11 to work in forward rotation. Since the output end of the forward and reverse motor 31 is fixedly connected to the rotating frame 32, the interior of the rotating frame 32 is used to fix the electromagnetic valve 33, such as Figure 8 As shown, when the forward and reverse motor 1 31 is working in the forward direction, it will drive the rotating frame 32, the solenoid valve 1 33 and the pipe 1 34 fixedly connected to the solenoid valve 1 33 to rotate slowly;

[0045] Step 4: The semicircular plate 329 fixedly connected to the outer side of the pipe 5 318 is arranged on the rotation path of the pipe 1 34, as shown in FIG. Figure 8As shown, when the pipe 34 partially enters the semicircular plate 329, the forward and reverse motor 31 stops working, and the pneumatic cylinder 322 starts working. After the pneumatic cylinder 322 starts working, the semicircular plate 329 stops working. In this process, after the forward and reverse motor 31 stops working, the pipe 34 stops moving and is aligned with the pipe 5 318.

[0046] When the pneumatic cylinder 1 322 is working, the piston end of the pneumatic cylinder 1 322 to which the mounting frame 1 311 is fixedly connected is fixedly connected to the mounting frame 2 323, and the mounting frame 2 323 is fixedly connected to the pipe fitting 5 318, such as Fig.13 As shown, the pneumatic cylinder 1 322 will push the pipe 5 318 to move toward the pipe 1 34 through the mounting frame 2 323. A rubber pad 1 320 is arranged inside the inner ring groove 319 opened inside the pipe 5 318. Fig.14 As shown, the moving pipe fitting 5 318 drives the fixedly connected rubber pad 1 320 to move toward the outside of the pipe fitting 1 34. Since the outer ring groove 35 is provided on the outer side of the pipe fitting 1 34, the rubber pad 1 320 finally enters the inner part of the outer ring groove 35, and the elastically deformed rubber pad 1 320 ensures the sealing between the pipe fitting 1 34 and the pipe fitting 5 318. After the end of the pipe fitting 1 34 is inserted into the inner ring groove 319, it contacts the pipe fitting 5 318, so the inside of the pipe fitting 1 34 is connected with the inside of the pipe fitting 5 318 and forms a relatively smooth cavity.

[0047] Step 5: After the pneumatic cylinder 1 322 stops working, the electromagnet 312 stops working, the forward and reverse motor 1 31 continues to work forward, and the pipe 1 34 continues to rotate. Since the two mounting frames 1 311 that are fixedly connected to the two pneumatic cylinders 1 322 are fixedly connected with the sliding member 2 310, as shown in FIG. Fig.12 As shown, the sliding member 2 310 is slidably connected with the sliding member 1 39, so after the electromagnet 312 stops working and the mounting frame 1 311 loses its fixation, the sliding member 2 310 is in a state where it can slide inside the sliding member 1 39. At this time, when the pipe 1 34 rotates, it will drive the pipe 5 318 to rotate, and the top of the outer side of the pipe 5 318 is fixedly connected with a fixing ring 321, and the outer walls of the two air pressure cylinders 1 322 penetrate the fixing ring 321, as shown in FIG. Fig.12 As shown, after the pipe 5 318 is stressed, the pipe 5 318 drives the two pneumatic cylinders 1 322 to move through the fixing ring 321, and the pneumatic cylinder 1 322 drives the sliding member 2 310 to move through the mounting frame 1 311. Since the sliding member 1 39 is arc-shaped, the sliding track of the sliding member 2 310 inside the sliding member 1 39 and the rotating track of the pipe 1 34 are two concentric circles, one large and one small, so the sliding member 2 310, the pipe 5 318 and the pipe 1 34 rotate synchronously;

[0048] Step 6: After the pipe 1 34, the sliding member 2 310, and the pipe 5 318 rotate to be perpendicular to the ground, the forward and reverse motor 1 31 stops working for the second time, and the rotation of the pipe 1 34, the sliding member 2 310, and the pipe 5 318 stops; the pipe 6 327 is provided through the two mounting frames 1 311, as shown in FIG. Fig.13 As shown, after the sliding member 2 310 stops moving and becomes perpendicular to the ground, the two pipe members 6 327 fixed by the two mounting frames 1 311 are aligned with the two circular holes 339 opened in the sliding member 1 39;

[0049] After the forward and reverse motor 1 31 stops working for the second time, the pneumatic cylinder 3 344 in the human-machine interactive device controls the hydraulic system 3 to work. Since the mounting frame 6 343 is fixedly connected between the two pipes 7 337, the pneumatic cylinder 3 344 is fixedly connected between the mounting frame 6 343 and the sliding member 1 39. Figure 7 As shown, after the air cylinder 344 is retracted, the mounting frame 6 343 drives the two pipe fittings 7 337 to move upward, and the top ends of the two pipe fittings 7 337 extend to the inside of the two circular holes 339 respectively. The inside of the pipe fitting 7 337 is fixedly connected with a rubber pad 2 338, as shown in FIG. Fig.11 As shown, after the pipe fitting seven 337 is moved up, the two pipe fittings seven 337 are sleeved onto the outside of the two pipe fittings six 327, and the elastically deformed rubber pad two 338 ensures the sealing between the pipe fitting seven 337 and the pipe fitting six 327, avoiding the residual diluent inside the pipe fitting one 34 from spilling during subsequent work, thereby reducing environmental pollution.

[0050] Step 8, the bottoms of the two pipe fittings 337 are fixedly connected to hoses 336, and the bottoms of the two hoses 336 are fixedly connected to water pumps 335. Fig. 9 As shown, a pipe fitting eight 345 is fixedly connected to one side of the water pump 335; among the two water pumps 335, the water inlet end of one water pump 335 is fixedly connected to the hose 336, and the pipe fitting eight 345 connected to the water outlet end of the water pump 335 is communicated with the wastewater collection point, and this water pump 335 is a wastewater pump 335; the water outlet end of the other water pump 335 is connected to the hose 336, and the pipe fitting eight 345 connected to the water inlet end of the water pump 335 is communicated with the cleaning liquid storage point, and this water pump 335 is a cleaning water pump 335; because the stirring member 317 rotatably connected inside the pipe fitting five 318 is fixedly connected to two solenoid valves two 347, the two solenoid valves two 347 are communicated with the guide box 324 fixedly connected to the stirring member 317, as shown in FIG. Fig.14 As shown, the rotating ring 325 rotatably connected inside the guide box 324 is fixedly connected with a telescopic tube 1 326, the telescopic tube 1 326 is fixedly connected with the pipe 6 327, and the waste water pump 335 and the cleaning water pump 335 are respectively connected with two solenoid valves 2 347;

[0051] After the pneumatic cylinder three 344 works, a cleaning operation is performed. At this time, the cleaning water pump 335 and the solenoid valve two 347 connected to the cleaning water pump 335 work. The cleaning water pump 335 extracts the cleaning liquid and transports it to the inside of the pipe five 318 through the hose 336, the pipe seven 337, and the solenoid valve two 347. The cleaning liquid transported by the cleaning water pump 335 enters the cavity formed by the pipe one 34 and the pipe five 318; the forward and reverse motor two 315 is controlled to work, and the working forward and reverse motor two 315 drives the stirring member 317 fixedly connected to the output end to rotate The rotating stirring member 317 stirs the cleaning liquid inside the pipe 5 318 and the pipe 1 34 to clean the inside of the pipe 1 34. The rotating stirring member 317 makes the cleaning liquid move to ensure the quality of cleaning the inside of the pipe 1 34. After a period of time, the cleaning water pump 335 and the electromagnetic valve 2 347 connected to the cleaning water pump 335 stop working, and the waste water pump 335 and the electromagnetic valve 2 347 connected to the waste water pump 335 work to extract and discharge the liquid inside the pipe 5 318 and the pipe 1 34, completing a cleaning work.

[0052] After performing the cleaning work twice or three times according to the above steps, the human-machine interaction device controls the two solenoid valves 2 347 to stop working and close, controls the pneumatic cylinder 344 to work and pushes the pipe 7 337 to reset, so that the pipe 6 327 loses the limit, and the mounting frame 1 311 can resume the motion state, and then controls the forward and reverse motor 1 31 to reverse work, so that the pipe 1 34, the pipe 5 318 and the sliding member 2 310 rotate and reset. When the mounting frame 1 311 moves and aligns with the electromagnet 312, the forward and reverse motor 1 31 stops working, and the electromagnet 312 is fixed to the mounting frame 1 311 through magnetic force. The mounting frame 1 311 and the sliding member 2 310 fixedly connected to the mounting frame 1 311 are fixed, and then the pneumatic cylinder 1 322 contracts, and the pipe 5 318 is separated from the pipe 1 34. Then the forward and reverse motor 1 31 continues to work to reset the pipe 1 34 and prepare for the next work.

[0053] When the liquid path system 3 in the present application supplies diluent to the immunoanalyzer structure 1, it is ensured as much as possible that the diluent sucked and used by the immunoanalyzer structure 1 is clean each time, and the pipe 34 for temporarily storing the diluent is cleaned to ensure the accuracy of the fluorescence analysis of the blood sample, and a fixed amount of diluent is supplied multiple times according to the number of multiple blood samples to reduce the waste of diluent, avoid the situation where the diluent is used after being in contact with the air for a long time, and reduce the situation where the blood sample analysis is inaccurate due to the quality change of the diluent.

[0054] In addition, such as Fig.13 and Fig.14As shown, a sliding connection assembly is provided between the forward and reverse motor 2 315 and the pipe 313 to which the stirring member 317 is fixedly connected. Since the pipe 313 is fixedly connected to the sliding member 2 310 in the sliding connection assembly, the distance between the pipe 313 and the sliding member 1 39 remains unchanged. The pipe 4 316 provided inside the pipe 313 is fixedly sleeved on the outside of the forward and reverse motor 2 315. The output end of the forward and reverse motor 2 315 is fixedly connected to the stirring member 317. When the pipe 5 318 approaches or moves away from the pipe 1 34, the pipe 5 318 will drive the stirring member 317 to move synchronously, and the forward and reverse motor 2 315 will move synchronously. The telescopic pipe 1 326 that can be telescoped meets the synchronous movement requirements of the guide box 324 and the stirring member 317.

[0055] When the forward and reverse motor 2 315 drives the stirring member 317 to rotate, since the pipe 4 316 cannot rotate inside the pipe 3 313, the forward and reverse motor 2 315 itself cannot rotate, and the rotating stirring member 317 drives the guide box 324 to rotate, but the rotating ring 325 can rotate inside the guide box 324, so the telescopic tube 1 326 will not be affected by the rotation of the stirring member 317;

[0056] In addition, a telescopic rod 1 314 is fixedly connected between the forward and reverse motor 2 315 and the sliding member 2 310 , and the telescopic rod 1 314 can be telescoped to prevent the forward and reverse motor 2 315 from being disengaged, thereby ensuring the stable operation of the forward and reverse motor 2 315 .

[0057] In addition, such as Figure 6 and Figure 8 As shown, the base 11 is fixedly connected to the mounting frame 7 346, the pipe fitting 8 345 passes through the mounting frame 7 346 and is fixedly connected to the mounting frame 7 346, and the mounting frame 7 346 supports the pipe fitting 8 345 to ensure the stable operation of the pipe fitting 8 345.

[0058] In addition, such as Fig.13 As shown, a telescopic rod 2 328 is fixedly connected between the mounting frame 1 311 and the rotating ring 325, and the telescopic rod 2 328 prevents the distortion force generated by the rotation of the guide box 324 from acting on the telescopic tube 1 326, thereby ensuring that the telescopic tube 1 326 can be stably telescoped, and a pipe fitting 6 327 passes through the mounting frame 1 311 and is fixedly connected to the mounting frame 1 311, so that the pipe fitting 6 327 and the mounting frame 1 311 are integrated, thereby ensuring that the relative position between the pipe fitting 6 327 and the mounting frame 1 311 remains unchanged.

[0059] In addition, such as Figure 6 and Fig.10As shown, a mounting frame 330 is fixedly connected to the bottom of the base 11, and multiple mounting frames 433 slidably connected to the mounting frame 330 are all fixedly connected to the sliding member 1 39, so the sliding member 1 39 can slide on one side of the mounting frame 330. Since the mounting frame 4 333 is set in an arc shape, the movement trajectory of the sliding member 1 39, the movement trajectory of the sliding member 2 310, and the movement trajectory of the pipe 1 34 are concentric circles of different sizes. One end of the operating bolt 331 threadedly connected to the mounting frame 330 is rotatably connected to the clamping plate 332, so the position of the clamping plate 332 can be controlled by rotating the operating bolt 331. The clamping plate 332 is slidably connected to the mounting frame 330, and the clamping plate 332 can only move horizontally. In a normal state, the clamping plate 332 and the multiple mounting frames 4 333 are tightly attached to limit their positions.

[0060] When necessary, the operating bolt 331 can be rotated to make the clamping plate 332 away from the mounting frame 4 333, so that the mounting frame 4 333 and the sliding member 1 39 lose their fixation. Since the end of the sliding member 1 39 away from the mounting frame 330 is fixedly connected to the guide rail 340, and one end of the guide ball 341 arranged inside the guide rail 340 is rotatably connected to the pneumatic cylinder 2 342, the pneumatic cylinder 2 342 can be controlled to extend and contract, so that the pneumatic cylinder 2 342 controls the position of the guide ball 341, so that the guide ball 341 drives the guide rail 340 and the sliding member 1 39 to move, and the sliding member 1 39 moves. 139 can reciprocate. When the electromagnet 312 is in working state, the mounting frame 1311, the sliding piece 2 310, the pipe piece 5 318, the pipe piece 313 and the sliding piece 139 move synchronously, and the pneumatic cylinder 2 342 can be controlled to work. When the robot arm works to clamp the Tip head to absorb the diluent from the inside of the pipe piece 134, the sliding piece 139 is controlled to slide away from the top of the base 11. When the robot arm leaves the vicinity of the liquid circuit system 3, the sliding piece 139 is reset when the pneumatic cylinder 2 342 works, so as to avoid the setting of the sliding piece 139 affecting the normal operation of the robot arm;

[0061] Both hoses 336 are provided with a certain reserved margin to meet the need for synchronous movement of pipe fitting seven 337 and sliding member one 39, avoid movement of the two water pumps 335 fixedly installed by mounting frame three 330 through mounting frame five 334, and ensure the safety of pipe fitting eight 345 fixedly installed by mounting frame seven 346.

[0062] In addition, such as Figure 6 and Fig.10As shown, the clamping frame 24 is fixedly connected to a three-way valve 28, and both sides of the air guide tube 27 fixedly connected to the three-way valve 28 are fixedly connected to multiple clamping members 26, and the multiple clamping members 26 are respectively arranged inside the multiple sample placement slots 25 opened in the clamping frame 24. When the blood sample container is placed, the control air pump 29 works to infuse air into the multiple clamping members 26 through the three-way valve 28 and the air guide tube 27, so that the clamping members 26 expand to clamp and fix the blood sample container. When the blood sample container needs to be replaced, the control air pump 29 stops working, and the normally closed end of the three-way valve 28 is controlled to open. At this time, the internal gas of the clamping member 26 and the air guide tube 27 is discharged through the three-way valve 28, and the blood sample container loses its fixation.

Claims

1. A fluid path system of a fluorescent immunoassay analyzer, comprising an immunoassay analyzer structure (1), characterized in that: The immunoassay analyzer structure (1) comprises a base (11) and a square groove (15); a fluid path system (3) and a sample support structure (2) are installed on the immunoassay analyzer structure (1); the square groove (15) is formed on the top of the base (11); a portion of the fluid path system (3) is arranged on the top of the base (11) through the square groove (15); and the sample support structure (2) is installed on the top of the base (11); The liquid circuit system (3) comprises a forward and reverse motor (31) and a sliding member (39); a rotating frame (32) is installed at the output end of the forward and reverse motor (31); a solenoid valve (33) is fixedly installed inside the rotating frame (32); an input end of the solenoid valve (33) is connected to a liquid storage tank (38); an output end of the solenoid valve (33) is fixedly connected to a pipe (34); an outer ring groove (35) is provided on the outer side of the pipe (34); The inner recess of the sliding member 1 (39) is slidably connected to the sliding member 2 (310), and the two sides of the sliding member 2 (310) are fixedly connected to the mounting frame 1 (311), and the mounting frame 1 (311) is provided with an electromagnet (312) on the side close to the sliding member 1 (39), and the electromagnet (312) is fixedly connected to the sliding member 1 (39), and the two mounting frames 1 (311) are fixedly connected to the pneumatic cylinder 1 (322), and a pipe fitting 5 (318) is fixedly installed between the two pneumatic cylinders 1 (322), and the pipe fitting 5 (318) is rotatably connected to the stirring rod. The stirring member (317) is fixedly connected to two electromagnetic valves (347), the stirring member (317) is fixedly connected to a guide box (324), a rotating ring (325) is rotatably connected inside the guide box (324), the rotating ring (325) is fixedly connected to a telescopic tube (326), and the telescopic tube (326) is fixedly connected to a pipe fitting (327); the stirring member (317) is fixedly connected to a forward and reverse motor (315), and a sliding connection assembly is provided between the forward and reverse motor (315) and the sliding member (310); The fluid circuit system (3) comprises two pipe fittings (7) (337), a mounting frame (6) (343) is fixedly connected between the two pipe fittings (7) (337), and a pneumatic cylinder (344) is fixedly connected between the mounting frame (6) (343) and a sliding member (39).

2. The liquid path system of a fluorescent immunoassay analyzer according to claim 1, characterized in that: The immunoassay analyzer structure (1) comprises a centrifugal module (12), a reagent chamber (13), a tip head assembly (14) and a housing (16); the housing (16) is mounted on one side of the top of the base (11) and is fixedly connected to a liquid storage tank (38); and the centrifugal module (12), the reagent chamber (13) and the tip head assembly (14) are all mounted on the top of the base (11).

3. The liquid path system of a fluorescent immunoassay analyzer according to claim 1, characterized in that: The sample support structure (2) comprises a U-shaped support frame (21), a pressure sensor (22) is arranged inside the U-shaped support frame (21), a plurality of elastic members (210) are fixedly connected inside the U-shaped support frame (21), and a pad (211) is fixedly connected to the bottom of the elastic member (210).

4. The liquid path system of a fluorescent immunoassay analyzer according to claim 3, characterized in that: The sample support structure (2) comprises a clamping frame (24), and a plurality of mounting bent rods (23) are fixedly connected between the base (11) and both sides of the clamping frame (24). A plurality of sample placement grooves (25) are provided on the top of the clamping frame (24) and the top of the U-shaped support frame (21), and the plurality of sample placement grooves (25) provided on the top of the U-shaped support frame (21) correspond one-to-one to the plurality of elastic members (210).

5. The liquid path system of a fluorescent immunoassay analyzer according to claim 4, characterized in that: The clamping frame (24) is fixedly connected to a three-way valve (28), the three-way valve (28) is fixedly connected to an air guide tube (27), both sides of the air guide tube (27) are fixedly connected to a plurality of clamping members (26), and the normally open end of the three-way valve (28) is fixedly connected to an air pump (29).

6. The liquid path system of a fluorescent immunoassay analyzer according to claim 1, characterized in that: The forward and reverse motor 1 (31) is fixedly connected to the base (11); a semicircular plate (329) is fixedly connected to the outer side of the pipe fitting 5 (318); a fixing ring (321) is fixedly connected to the outer top of the pipe fitting 5 (318); the outer wall of the pneumatic cylinder 1 (322) passes through the fixing ring (321); the piston end of the pneumatic cylinder 1 (322) is fixedly connected to a mounting frame 2 (323); the mounting frame 2 (323) is fixedly connected to the pipe fitting 5 (318); the input end of the solenoid valve 1 (33) is rotatably connected to the pipe fitting 2 (36); one end of the pipe fitting 2 (36) is fixedly connected to a flow controller (37); the flow controller (37) is fixedly connected to a liquid storage tank (38).

7. The liquid path system of a fluorescent immunoassay analyzer according to claim 1, characterized in that: The sliding connection assembly includes a pipe fitting three (313), the pipe fitting three (313) is fixedly connected to the sliding member two (310), a pipe fitting four (316) is arranged inside the pipe fitting three (313), the pipe fitting four (316) is fixedly sleeved on the outside of the forward and reverse motor two (315), a telescopic rod one (314) is fixedly connected between the forward and reverse motor two (315) and the sliding member two (310), and the telescopic rod one (314) is arranged inside the pipe fitting four (316).

8. The liquid path system of a fluorescent immunoassay analyzer according to claim 1, characterized in that: The electromagnet (312) is fixedly connected to the sliding member 1 (39); a telescopic rod 2 (328) is fixedly connected between the mounting frame 1 (311) and the rotating ring (325); the pipe member 6 (327) passes through the mounting frame 1 (311) and is fixedly connected to the mounting frame 1 (311); the solenoid valve 2 (347) is communicated with the inside of the guide box (324); an inner ring groove (319) is provided inside the pipe member 5 (318); a rubber pad 1 (320) is provided inside the inner ring groove (319); and the rubber pad 1 (320) is fixedly connected to the pipe member 5 (318).

9. The liquid path system of a fluorescent immunoassay analyzer according to claim 1, characterized in that: The sliding member 1 (39) has two circular holes (339) at the top, the tops of the two pipe fittings 7 (337) extend into the two circular holes (339) respectively, the pipe fitting 7 (337) is fixedly connected with a rubber pad 2 (338) inside, the bottoms of the two pipe fittings 7 (337) are fixedly connected with a hose (336), the bottoms of the two hoses (336) are fixedly connected with a water pump (335), one side of the water pump (335) is fixedly connected with a pipe fitting 8 (345), the base (11) is fixedly connected with a mounting frame 7 (346), and the pipe fitting 8 (345) passes through the mounting frame 7 (346) and is fixedly connected to the mounting frame 7 (346).

10. The liquid path system of a fluorescent immunoassay analyzer according to claim 9, characterized in that: The bottom of the base (11) is fixedly connected with a mounting frame three (330), and the mounting frame three (330) is threadedly connected with an operating bolt (331), and one end of the operating bolt (331) is rotatably connected with a clamping plate (332). The mounting frame three (330) is slidably connected with multiple mounting frames four (333), and the multiple mounting frames four (333) are all fixedly connected with a sliding member one (39). A mounting frame five (334) is fixedly connected between the mounting frame three (330) and two water pumps (335). The sliding member one (39) is fixedly connected with a guide rail (340) at one end away from the mounting frame three (330), and a guide ball (341) is arranged inside the guide rail (340). One end of the guide ball (341) is rotatably connected with a pneumatic cylinder two (342), and the pneumatic cylinder two (342) is fixedly connected with the mounting frame seven (346).

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