Method for cultivating bud seedlings from broccoli seeds containing high-content sulforaphane

Abstract

The invention relates to a method for cultivating buds and seedlings from broccoli seeds containing high-content sulforaphane, and belongs to the technical field of food. The method for cultivating bud seedlings from broccoli seeds containing high-content sulforaphane comprises the following steps: step 1, selecting seeds; step 2, live broadcast; step 3, irrigation; step 4, illumination; step 5, temperature control; 6, daily management of seedling raising; 7, disease and pest control; 8, the production environment is kept sanitary; step 9, harvesting. According to different illumination periods of plants, illumination starting time and corresponding temperature and humidity change setting are changed, the optimal environment is created according to plant growth requirements, the plants are completely in a natural growth state, and broccoli bud seedlings which are fresh and alive for 5-10 days are obtained.

Description

Technical Field

[0001] The invention relates to a method for cultivating sprouts from broccoli seeds containing high content of sulforaphane, and belongs to the technical field of food. Background Art

[0002] Broccoli, Latin name: Brassica oleracea L.var.italic P l anch., is a biennial herbaceous plant of the genus Brassica in the family Cruciferae. The nutrients in broccoli are not only high in content but also very comprehensive, mainly including protein, carbohydrates, fat, minerals, vitamin C and carotene.

[0003] Broccoli sprouts are rich in sulforaphane and are also known as super anti-cancer foods. Sulforaphane exists in high concentrations in the form of its precursor glucoraphanin in cruciferous vegetables, such as broccoli, cabbage, kale and Brussels sprouts. The change in the content of glucoraphanin in broccoli sprouts is affected by many factors, such as variety, germination conditions, external environment, etc., and their content directly affects the content of sulforaphane.

[0004] In the prior art, the cultivation of broccoli sprouts from broccoli seeds has the disadvantages of low sulforaphane content, complex process, high cost, etc. Therefore, it is necessary to study a new method for cultivating broccoli sprouts from broccoli seeds containing high sulforaphane content to increase the sulforaphane content in broccoli sprouts. Summary of the invention

[0005] The object of the present invention is to provide a method for cultivating sprouts from broccoli seeds containing high content of sulforaphane.

[0006] The technical solution of the present invention to solve the above technical problem is as follows: A method for cultivating sprouts from broccoli seeds containing high content of sulforaphane comprises the following steps:

[0007] Step 1: Seed Selection

[0008] Take broccoli seeds, first sterilize them with ultraviolet light, then spray them with disinfectant to obtain sterilized seeds;

[0009] Step 2: Live Broadcast

[0010] Spread sprout paper or sprout gauze on the seedling tray, and evenly sow the sterilized seeds obtained in step 1 on the seedling tray; then use a seeder to sow;

[0011] Step 3: Irrigation

[0012] On the first 1-2 days, spray irrigation is carried out; the humidity is controlled at 80%-90% during the day and 50%-60% at night; on the 3-10th day, alternate spray and tidal irrigation is used, the humidity is controlled at 60%-70% during the day and 50%-60% at night; pure water is used throughout the irrigation process;

[0013] Step 4: Lighting

[0014] Seed germination period: On the 1st to 2nd day, cultivate in a dark room without light;

[0015] Seedling growth period: on the 3rd to 4th day of seed germination, light intensity is 2000l ux-4000l ux, and light time is 12h-16h;

[0016] Growth period: On the 5th to 10th day of the broccoli sprouts, turn on the UV lamp independently every day. The power of the UV lamp is 20W-50W, and the irradiation time is 1min-9min per day.

[0017] Step 5: Temperature Control

[0018] Seed germination period: On the first 2 days, the daytime temperature is maintained at 25℃-35℃; the nighttime temperature is maintained at 15℃-17℃;

[0019] Seedling growth period: the daytime temperature is maintained at 20℃-28℃, and the nighttime temperature is maintained at 16℃-20℃;

[0020] Step 6: Daily management of seedlings

[0021] Check the growth of seedlings in the seedling tray, the temperature, humidity, light, alternating irrigation, fresh air, circulating air and automation control of the nursery room every day, and combine manual records with IoT monitoring logs;

[0022] Step 7: Pest and disease control;

[0023] Step 8: Closed management;

[0024] Step 9: Harvest.

[0025] The principle of the method for cultivating sprouts from broccoli seeds containing high content of sulforaphane of the present invention is:

[0026] The germination method of the prior art is to soak the seeds for several hours, then dry them, store them at natural room temperature or refrigerate them, and sprinkle them with water for several days. In step 2 of the present invention, the broccoli seeds are not soaked, and the seeds are directly and evenly sown on a standard seedling tray, and the seedling tray is paved with sprout paper or sprout gauze. Each seedling tray uses a sowing machine for automatic precision sowing. This direct sowing method using a sowing machine in conjunction with a seedling tray not only improves the speed of direct sowing, but also helps each seed to enter the hole and keep a certain amount of pure water to soak the seeds after watering, which has the effect of accurate wall breaking and germination.

[0027] In step 3 of the present invention, irrigation is performed by combining spray irrigation with tidal irrigation. From the third day, spray irrigation is performed once every 2 hours. As the seedlings gradually grow and become stronger, the interval time of spray irrigation is extended by 1 hour every day, which correspondingly reduces the amount of water attached to the seedlings and improves the effect of photosynthesis. The spray irrigation time is reduced from 15 seconds each time by 1 second every day throughout the 8-day growth process, thereby increasing the yield of purple orchid seedlings and increasing the sulforaphane content. Spray irrigation can be evenly sprayed on each seed, so that the seedlings can obtain an appropriate amount of water, while keeping the air of the overall environment moist, and controlling the amount of water sprayed. Tidal irrigation ensures that each seed can obtain appropriate water no matter where it is located in the seedling tray, controls the supply and discharge of water, ensures the respiration and oxygen permeability of the root of the seedlings, and avoids causing black rot of the seedlings. Scientifically proportion time, scientific regulation of day and night temperature and humidity, so as to achieve the peak period of optimal photosynthesis of plants and increase the content of sulforaphane, and increase the yield of seedlings.

[0028] In step 4 of the present invention, sufficient light is required for the growth of sprouts, but too strong light will reduce the glucoraphanin contained in the sprouts. It is necessary to control the light intensity and time during the production process to ensure that the broccoli seeds are carefully guarded from shelling, germination, and sprout growth. After 5-9 days of careful protection, the scientifically equipped supplementary light provides scientific and accurate light for the broccoli seeds, so as to achieve the effect of plant growth in natural conditions and ensure that the sulforaphane content reaches the optimal content. Specifically, the illumination time is gradually increased. During the seed germination period, weak light needs to be provided, and no additional supplementary light is required. During the seedling growth period, sufficient light needs to be provided. During the growth period, ultraviolet light can be used to disinfect, sterilize, stimulate plant growth, increase nutrients, and promote the quality of sprouts. Experimental data show that ultraviolet light increases the sulforaphane content during the growth of broccoli sprouts. A 20W lamp is added every two days. The light irradiation starts for 6 hours. For every 24 hours, the light irradiation is increased by 1 hour, and the final irradiation time per day is increased to 13 hours. On the first and a half days, turn on 1 light tube; on the second day, turn on 2 tubes, and add 1 light tube for every additional 24 hours.

[0029] In step 5 of the present invention, broccoli sprouts prefer a warm environment, and the suitable temperature is 20-28° C. Through system control, the required temperature during the seed germination period and the seedling growth period can be accurately controlled and maintained, which can promote the growth and development of the seedlings.

[0030] In step 7 of the present invention, the sprouts are susceptible to pests and diseases, and physical methods are used in the production process to control the occurrence of diseases by adjusting the temperature and humidity, water irrigation time, and appropriate ultraviolet irradiation disinfection methods in a closed and purified growth space. No disinfectant is used to ensure natural green.

[0031] In step 8 of the present invention, sprout production needs to maintain the hygiene of the production environment, regularly use ultraviolet disinfection, clean sprout production equipment and sites, avoid the growth of germs and bacteria, and the specific operation process, time period, and duration are performed in accordance with the following requirements: In the sprout culture workshop, a 300,000-level aseptic state and automated intelligent operation are achieved, and the implementation standards are: GB 14850-2017 "Food Production Workshop Hygiene Standards" and GB 2762-2017 "National Food Safety Standard Limits of Contaminants in Food".

[0032] In step 9 of the present invention, after harvesting, the yield of broccoli sprouts in each seedling tray is 250g-350g, and the content of sulforaphane is increased. In the prior art method, the yield of broccoli sprouts in each seedling tray is 200g. Therefore, by using the method of dynamic irrigation and dynamic lighting at different periods of the present invention, the yield of broccoli sprouts is increased. Correspondingly, the content of sulforaphane is also increased.

[0033] In summary, the present invention changes the light on time and the corresponding temperature and humidity setting according to the different light cycles of plants, creates the best environment according to the growth needs of plants, and makes the plants completely in a natural growth state, thereby obtaining 5-10 day fresh broccoli sprouts. The 5-10 day fresh broccoli sprouts have a relatively high sulforaphane content, and the sulforaphane content of a single sprout is increased by 5%-40%, which is more suitable for the subsequent preparation of natural active sulforaphane.

[0034] The beneficial effects of the method for cultivating sprouts from broccoli seeds containing high content of sulforaphane of the present invention are:

[0035] 1. The present invention changes the light on time and the corresponding temperature and humidity change settings according to the different light cycles of plants, creates the best environment according to the growth needs of plants, and makes the plants completely in a natural growth state, so as to obtain 5-10 days fresh broccoli sprouts. The weight of broccoli sprouts is 8-12 times the weight of seeds, and the 5-10 days fresh broccoli sprouts have a relatively high sulforaphane content. The sulforaphane content of a single sprout is increased by 5%-40%, which is more suitable for the subsequent preparation of natural active sulforaphane.

[0036] 2. The method of the present invention is simple, easy to operate, low-cost, has broad market prospects, and is suitable for large-scale promotion and application.

[0037] Based on the above technical solution, the present invention can also be improved as follows.

[0038] Furthermore, in step 1, the power of the ultraviolet disinfection is 20W-50W, and the time is 3h-6h.

[0039] The above further beneficial effect is: the present invention has found through research that ultraviolet disinfection can effectively remove pests and diseases on the seed surface, and can achieve the purpose of fully disinfecting the seed shell.

[0040] Furthermore, in step 1, the disinfectant spraying is specifically: using hydrogen peroxide silver ion disinfectant, spraying for 10min-15min.

[0041] A further beneficial effect of the above method is that after ultraviolet disinfection, spraying with hydrogen peroxide silver ion disinfectant can make the seeds swell, which is more conducive to subsequent direct seeding.

[0042] The above hydrogen peroxide silver ion disinfectant can be purchased commercially, such as from Huwa-San of ROAM Company in Belgium, with specification of TR50.

[0043] Further, in step 2, the size of the seedling tray is 27 cm long × 54 cm wide × 6 cm high.

[0044] Further, in step 2, the seeder sows 4500-5000 seeds each time.

[0045] The above-mentioned further beneficial effect is that each seedling tray uses a seeder to automatically and precisely sow the seeds. This direct seeding method using a seeder in conjunction with a seedling tray not only increases the speed of direct seeding, but also helps each seed enter the hole, and maintains a certain amount of pure water to soak the seeds after watering, thereby achieving the effect of accurately breaking the wall and accelerating germination.

[0046] Furthermore, in step 3, the atomizing sprinkler irrigation is specifically: once per hour, each time for 15 seconds, and the amount of water used in each seedling tray each time is 16 mL.

[0047] Furthermore, in step 3, the tidal irrigation is specifically as follows: the amount of water used for each seedling tray is 2.5 L each time.

[0048] Further, in step 4, the lighting uses an LED plant growth lamp with a power of 20W, and the luminous flux of each LED is ≥40μmo l / s; IP protection level: moisture-proof, IP65; lamp tube length: 1200mm; spectrum: full spectrum, in which blue light is included in white light, the red light peak is 660nm, the blue light peak is 450nm, the far-red light peak is 735nm, and the fluctuation range of the above wavelengths is ≤5%; cooling method: air heat dissipation.

[0049] The further beneficial effect of adopting the above is that the LED plant growth lamp, with a spectrum containing red / blue / white / far-red, can enable each stage of plant growth to receive the most suitable light for that stage, and can ensure high uniformity of light, so that no matter where the plant is in the culture rack, it can obtain the same level of light. This makes the plants grow at the same rate and in the same way. It is precisely because of the high quality assurance of LED plant growth lamps that the light output can be maintained at a very high level even after years of use. Furthermore, LED lighting consumes less power than traditional Philips fluorescent lamps (up to 70% of electricity can be saved) and produces almost no heat, so the lamp tube can be placed very close to the plant, which means that more planting layers can be set up in the same space. The service life of LED plant growth lamps is up to 25,000 hours, so there is no need to replace the lamp tube frequently, and there is no need to worry about the lamp tube being damaged. In addition, there is no need to add supporting facilities such as fans for passive heat dissipation.

[0050] The above-mentioned light source can be purchased commercially, such as from Philips Lighting, which is a high-illuminance plant light source.

[0051] Furthermore, in step 7, the specific method of pest control is: perform ultraviolet irradiation disinfection for 3min-5min every day, and the power of the ultraviolet lamp is 20W-50W; air shower disinfection, protective clothing and shoe covers are worn by employees before entering the workshop.

[0052] A further beneficial effect of adopting the above method is that pests and diseases can be effectively prevented and controlled by adopting the above method. DETAILED DESCRIPTION

[0053] The principles and features of the present invention are described below in conjunction with embodiments. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0054] Example 1

[0055] The method of cultivating sprouts from broccoli seeds containing high sulforaphane content in this embodiment comprises the following steps:

[0056] Step 1: Seed Selection

[0057] Take broccoli seeds, first sterilize them with ultraviolet light, the power of the ultraviolet light sterilization is 50W, the time is 3 hours, and then spray with disinfectant, the disinfectant spraying is specifically: use hydrogen peroxide silver ion disinfectant, spray for 10 minutes, and obtain disinfected seeds.

[0058] Step 2: Live Broadcast

[0059] Spread sprout paper or sprout gauze on the seedling tray, and evenly sow the sterilized seeds obtained in step 1 on the seedling tray, the size of which is 27 cm long × 54 cm wide × 6 cm high; then use a seeder, the seeder sowing 4500 seeds each time, for sowing.

[0060] Step 3: Irrigation

[0061] On the 1st to 2nd day, mist sprinkler irrigation was carried out once an hour for 15 seconds each time, and 16 mL of water was used for each seedling tray each time; the humidity was controlled at 80%-90% during the day and 50%-60% at night; on the 3rd to 10th day, mist and tidal irrigation were used alternately, and 2.5 L of water was used for each seedling tray each time; the humidity was controlled at 60%-70% during the day and 50%-60% at night; purified water was used throughout the irrigation process.

[0062] Step 4: Lighting

[0063] Seed germination period: On the 1st to 2nd day, cultivate in a dark room without light;

[0064] Seedling growth period: on the 3rd to 4th day of seed germination, light intensity is 2000 l ux, and light duration is 16 h;

[0065] Growth period: On the 5th to 10th day of broccoli sprouts, turn on the UV lamp independently every day. The power of the UV lamp is 20W and the irradiation time is 9 minutes per day.

[0066] The lighting adopts LED plant growth lamp with a power of 20W and a luminous flux of each LED ≥

[0067] 40μmo l / s; IP protection level: moisture-proof, IP65; lamp tube length: 1200mm; spectrum: full spectrum, in which blue light is included in white light, red light peak is 660nm, blue light peak is 450nm, far red light peak is 735nm, and the fluctuation range of the above wavelengths is ≤5%; cooling method: air heat dissipation.

[0068] Step 5: Temperature Control

[0069] Seed germination period: the daytime temperature is maintained at 25℃-35℃; the nighttime temperature is maintained at 15℃-17℃;

[0070] Seedling growth period: the daytime temperature is maintained at 20℃-28℃, and the nighttime temperature is maintained at 16℃-20℃.

[0071] Step 6: Daily management of seedlings

[0072] Seedling trays, nursery rooms, humidity and seed germination are checked daily, and manual records are combined with IoT monitoring logs.

[0073] Step 7: Pest and disease control. The specific methods of pest and disease control are: high-frequency fresh air and high ventilation, indoor air is updated every 5 minutes; 1 minute of ultraviolet irradiation disinfection is performed every day, and the power of the ultraviolet lamp is 50W; employees are disinfected by air showers, protective clothing and shoe covers before entering the workshop.

[0074] Step 8: Keep the production environment clean.

[0075] Step 9: Harvest.

[0076] Example 2

[0077] The method of cultivating sprouts from broccoli seeds containing high sulforaphane content in this embodiment comprises the following steps:

[0078] Step 1: Seed Selection

[0079] Take broccoli seeds, first sterilize them with ultraviolet light, the power of the ultraviolet light sterilization is 35W, the time is 4.5 hours, and then spray with disinfectant, the disinfectant spraying is specifically: use hydrogen peroxide silver ion disinfectant, spray for 102 minutes, and obtain disinfected seeds.

[0080] Step 2: Live Broadcast

[0081] Spread sprout paper or sprout gauze on the seedling tray, and evenly sow the sterilized seeds obtained in step 1 on the seedling tray, the size of which is 27 cm long × 54 cm wide × 6 cm high; then use a seeder, the seeder sowing 4800 seeds each time, for sowing.

[0082] Step 3: Irrigation

[0083] On the 1st to 2nd day, mist sprinkler irrigation was carried out once an hour for 15 seconds each time, and 16 mL of water was used for each seedling tray each time; the humidity was controlled at 80%-90% during the day and 50%-60% at night; on the 3rd to 10th day, mist and tidal irrigation were used alternately, and 2.5 L of water was used for each seedling tray each time; the humidity was controlled at 60%-70% during the day and 50%-60% at night; purified water was used throughout the irrigation process.

[0084] Step 4: Lighting

[0085] Seed germination period: On the 1st to 2nd day, cultivate in a dark room without light;

[0086] Seedling growth period: on the 3rd to 4th day of seed germination, light intensity is 3000 l ux, and light duration is 14 h;

[0087] Growth period: On the 5th to 10th day of the broccoli sprouts, turn on the UV lamp independently every day. The power of the UV lamp is 35W and the irradiation time is 5 minutes per day.

[0088] The lighting adopts LED plant growth lamp with a power of 20W and a luminous flux of each LED ≥

[0089] 40μmo l / s; IP protection level: moisture-proof, IP65; lamp tube length: 1200mm; spectrum: full spectrum, in which blue light is included in white light, red light peak is 660nm, blue light peak is 450nm, far red light peak is 735nm, and the fluctuation range of the above wavelengths is ≤5%; cooling method: air heat dissipation.

[0090] Step 5: Temperature Control

[0091] Seed germination period: the daytime temperature is maintained at 25℃-35℃; the nighttime temperature is maintained at 15℃-17℃;

[0092] Seedling growth period: the daytime temperature is maintained at 20℃-28℃, and the nighttime temperature is maintained at 16℃-20℃.

[0093] Step 6: Daily management of seedlings

[0094] Seedling trays, nursery rooms, humidity and seed germination are checked daily, and manual records are combined with IoT monitoring logs.

[0095] Step 7: Pest and disease control. The specific methods of pest and disease control are: high-frequency fresh air and high ventilation, indoor air is renewed every 8 minutes; 3 minutes of ultraviolet irradiation disinfection is carried out every day, and the power of the ultraviolet lamp is 35W; employees are disinfected by air showers, protective clothing and shoe covers before entering the workshop.

[0096] Step 8: Keep the production environment clean.

[0097] Step 9: Harvest.

[0098] Example 3

[0099] The method of cultivating sprouts from broccoli seeds containing high sulforaphane content in this embodiment comprises the following steps:

[0100] Step 1: Seed Selection

[0101] Take broccoli seeds, first sterilize them with ultraviolet light, the power of the ultraviolet light sterilization is 20W, the time is 6 hours, and then spray with disinfectant, the disinfectant spraying is specifically: use hydrogen peroxide silver ion disinfectant, spray for 15 minutes, and obtain disinfected seeds.

[0102] Step 2: Live Broadcast

[0103] Spread sprout paper or sprout gauze on the seedling tray, and evenly sow the sterilized seeds obtained in step 1 on the seedling tray, the size of which is 27 cm long × 54 cm wide × 6 cm high; then use a seeder, the seeder sowing amount is 5000 seeds each time, for sowing.

[0104] Step 3: Irrigation

[0105] On the 1st to 2nd day, mist sprinkler irrigation was carried out once an hour for 15 seconds each time, and 16 mL of water was used for each seedling tray each time; the humidity was controlled at 80%-90% during the day and 50%-60% at night; on the 3rd to 10th day, mist and tidal irrigation were used alternately, and 2.5 L of water was used for each seedling tray each time; the humidity was controlled at 60%-70% during the day and 50%-60% at night; purified water was used throughout the irrigation process.

[0106] Step 4: Lighting

[0107] Seed germination period: On the 1st to 2nd day, cultivate in a dark room without light;

[0108] Seedling growth period: on the 3rd to 4th day of seed germination, light intensity 4000 l ux, light duration 12 h;

[0109] Growth period: On the 5th to 10th day of the broccoli sprouts, turn on the UV lamp independently every day. The power of the UV lamp is 50W and the irradiation time is 1 minute per day.

[0110] The lighting adopts LED plant growth lamp with a power of 20W and a luminous flux of each LED ≥

[0111] 40μmo l / s; IP protection level: moisture-proof, IP65; lamp tube length: 1200mm; spectrum: full spectrum, in which blue light is included in white light, red light peak is 660nm, blue light peak is 450nm, far red light peak is 735nm, and the fluctuation range of the above wavelengths is ≤5%; cooling method: air heat dissipation.

[0112] Step 5: Temperature Control

[0113] Seed germination period: the daytime temperature is maintained at 25℃-35℃; the nighttime temperature is maintained at 15℃-17℃;

[0114] Seedling growth period: the daytime temperature is maintained at 20℃-28℃, and the nighttime temperature is maintained at 16℃-20℃.

[0115] Step 6: Daily management of seedlings

[0116] Seedling trays, nursery rooms, humidity and seed germination are checked daily, and manual records are combined with IoT monitoring logs.

[0117] Step 7: Pest and disease control. The specific methods of pest and disease control are: high-frequency fresh air and high ventilation, indoor air is updated every 10 minutes; 5 minutes of ultraviolet irradiation disinfection is carried out every day, and the power of the ultraviolet lamp is 20W; employees are disinfected by air showers, protective clothing and shoe covers before entering the workshop.

[0118] Step 8: Keep the production environment clean.

[0119] Step 9: Harvest.

[0120] Experimental Example 1

[0121] During the seedling growth period, the light duration was maintained at 12 h, and the daytime temperature was set at 20℃-25℃, 14℃-20℃ and 25℃-28℃, respectively. The yield of broccoli sprouts was measured, as shown in Table 1.

[0122] Table 1

[0123] temperature Yield date Light duration 20℃-25℃ 253.0g 2023.12.01 12h 14℃-20℃ 228.0g 2023.11.01 12h 25℃-28℃ 249.1g 2023.09.13 12h

[0124] As shown in Table 1, under the same lighting conditions, too high or too low daytime temperature will affect the yield. The daytime temperature is 14℃-20℃, or the daytime temperature is 20℃-25℃, compared with the daytime temperature of 14℃-20℃, the yield of broccoli sprouts is slightly lower. Therefore, the appropriate daytime temperature is 20℃-25℃.

[0125] Experimental Example 2

[0126] During the seedling growth period, the temperature was maintained at 14℃-26℃, and the light duration was set to 13.5h, 14℃-20℃ and 12h, respectively. The yield of broccoli sprouts was measured, as shown in Table 2.

[0127] Table 2

[0128] Light duration Yield date temperature 13.5h 242.8g 2023.09.26 14℃-26℃ 12h 249.6g 2023.09.13 14℃-26℃

[0129] As shown in Table 2, under the same temperature, the light duration of 13.5h, compared with 12h, the yield of broccoli sprouts is slightly lower. Therefore, the light duration should not be too long.

[0130] Experimental Example 3

[0131] During the seedling growth period, the temperature was maintained at 20℃-25℃, the light duration was 12h, and the irrigation methods used were irrigation, sprinkler irrigation and tidal irrigation. The yield of broccoli sprouts was measured respectively, as shown in Table 3.

[0132] Table 3

[0133]

[0134] As shown in Table 3, under the same temperature and light duration, the irrigation method of sprinkler irrigation plus tidal irrigation increased the yield of broccoli sprouts by 10.53-38.76% compared with the single sprinkler irrigation method. Therefore, the irrigation method of sprinkler irrigation plus tidal irrigation can increase the yield of broccoli sprouts.

[0135] In summary, the present invention changes the light on time and the corresponding temperature and humidity change settings according to the different light cycles of plants, creates the best environment according to the growth needs of plants, and makes the plants completely in a natural growth state, so as to obtain 5-10 days fresh broccoli sprouts. The weight of broccoli sprouts is 8-12 times the weight of seeds, and the 5-10 days fresh broccoli sprouts have a relatively high sulforaphane content. The sulforaphane content of a single sprout is increased by 5%-40%, which is more suitable for the subsequent preparation of natural active sulforaphane.

[0136] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for growing sprouts from broccoli seeds containing high sulforaphane content, characterized in that: The steps include: Step 1: Seed Selection Take broccoli seeds, first sterilize them with ultraviolet light, then spray them with disinfectant to obtain sterilized seeds; Step 2: Live Broadcast Spread sprout paper or sprout gauze on the seedling tray, and evenly sow the sterilized seeds obtained in step 1 on the seedling tray; then use a seeder to sow; Step 3: Irrigation On the first 1-2 days, mist irrigation was carried out; the humidity was controlled at 80%-90% during the day and 50%-60% at night; On the 3rd to 10th day, alternate irrigation with mist and tidal irrigation was used, with humidity controlled at 60%-70% during the day and 50%-60% at night; purified water was used throughout the irrigation process; Step 4: Lighting Seed germination period: On the 1st to 2nd day, cultivate in a dark room without light; Seedling growth period: on the 3rd to 4th day of seed germination, light intensity is 2000lux-4000lux, and light time is 12h-16h; Growth period: On the 5th to 10th day of the broccoli sprouts, turn on the UV lamp independently every day. The power of the UV lamp is 20W-50W, and the irradiation time is 1min-9min per day. Step 5: Temperature Control Seed germination period: On the first 2 days, the daytime temperature is maintained at 25℃-35℃; the nighttime temperature is maintained at 15℃-17℃; Seedling growth period: the daytime temperature is maintained at 20℃-28℃, and the nighttime temperature is maintained at 16℃-20℃; Step 6: Daily management of seedlings Check the growth of seedlings in the seedling tray, the temperature, humidity, light, alternating irrigation, fresh air, circulating air and automation control of the nursery room every day, and combine manual records with IoT monitoring logs; Step 7: Pest and disease control; Step 8: Closed management; Step 9: Harvest.

2. The method for growing sprouts from broccoli seeds containing high sulforaphane content according to claim 1, characterized in that: In step 1, the power of the ultraviolet disinfection is 20W-50W, and the time is 3h-6h.

3. The method for growing sprouts of broccoli seeds containing high sulforaphane content according to claim 1, characterized in that: In step 1, the disinfectant spraying specifically includes: using hydrogen peroxide silver ion disinfectant, spraying for 10min-15min.

4. The method for growing sprouts from broccoli seeds containing high sulforaphane content according to claim 1, characterized in that: In step 2, the size of the seedling tray is 27 cm long × 54 cm wide × 6 cm high.

5. The method for growing sprouts of broccoli seeds containing high sulforaphane content according to claim 1, characterized in that: In step 2, the seeder sows 4500-5000 seeds each time.

6. The method for growing sprouts from broccoli seeds containing high sulforaphane content according to claim 1, characterized in that: In step 3, the atomization sprinkler irrigation is specifically: once per hour, each time for 15 seconds, and the water consumption of each seedling tray each time is 16 mL.

7. The method for growing sprouts of broccoli seeds containing high sulforaphane content according to claim 1, characterized in that: In step 3, the tidal irrigation is specifically as follows: the amount of water used for each seedling tray is 2.5L each time.

8. The method for growing sprouts from broccoli seeds containing high sulforaphane content according to claim 1, characterized in that: In step 4, the lighting adopts LED plant growth lamp with a power of 20W, and the luminous flux of each LED is ≥40μmol / s; IP protection level: moisture-proof, IP65; lamp tube length: 1200mm; spectrum: full spectrum, in which blue light is included in white light, red light peak is 660nm, blue light peak is 450nm, far red light peak is 735nm, and the fluctuation range of the above wavelengths is ≤5%; cooling method: air heat dissipation.

9. The method for growing sprouts from broccoli seeds containing high sulforaphane content according to claim 1, characterized in that: In step 7, the specific method of pest control is: perform ultraviolet irradiation disinfection for 3min-5min every day, and the power of the ultraviolet lamp is 20W-50W; air shower disinfection, protective clothing and shoe covers are worn by employees before entering the workshop.

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