Preparation method of cotton straw fermented feed for cucurbita pepo seed production method

Abstract

The invention discloses a preparation method of a cotton straw fermented feed for a bottle gourd seed production method, which comprises the following steps: (1) expanding strains under a suitable condition without infectious microbes to prepare a high-density rejuvenation bacterium solution; (2) pulping and crushing seed summer squash pulp, adding high-density rejuvenation bacteria liquid and an enzyme preparation, uniformly mixing, and performing anaerobic fermentation to prepare seed summer squash pulp fermentation liquid; and (3) crushing cotton straws into powder, adding the seed cucurbita pepo pulp fermentation liquor and an enzyme preparation, uniformly mixing, and fermenting to obtain the cotton straw fermented feed prepared by the seed cucurbita pepo seed production method. The prepared cotton straw fermented feed prepared through the bottle gourd seed production method is rich in bioactive nutrients such as soluble fiber polysaccharide, soluble protein, active small peptide, organic acid, probiotics and biological enzyme, the palatability of straw feed is improved, and the fermented feed has the advantages of being high in food consumption, easy to digest and absorb, good in growth promoting performance and the like.

Description

Technical Field

[0001] The invention relates to the technical field of feed, and in particular to a method for preparing a cotton straw fermented feed in a gourd seed production method. Background Art

[0002] Seed zucchini is one of the main economic crops in Xinjiang, mainly used to produce zucchini seeds. The existing harvesting method is mainly to directly discard the skin and flesh after taking the seeds in the field and return them to the field as fertilizer. Since the discarded skin and flesh account for more than 90% of the weight of the whole fruit, it accumulates to millions of tons each year, which causes environmental pollution on the one hand and waste of resources on the other hand. A small amount can be directly fed to cattle and sheep by local residents in a short time or simply ensiled and fermented for feeding, but the above treatment methods cannot guarantee the long-term storage of the skin and flesh and the effective and large-scale utilization of the feed breeding industry. The fundamental reason for this phenomenon is that wild bacteria are naturally attached to the melon skin and the skin and flesh are rich in water, soluble sugars, proteins, fats, trace elements, etc., which are natural microbial culture media, so they are very easy to spoil. Secondly, since the water content of the melon flesh of the zucchini for western seeds reaches 90%, it is rich in nutrients and very suitable for microbial fermentation. However, the comprehensive cost of fermenting the melon flesh of the zucchini for western seeds is relatively high, and the comprehensive utilization is difficult.

[0003] In addition, as the most important cotton planting base in China, Xinjiang is rich in cotton straw resources. About 8-10 million tons of cotton straw are produced each year. Studies have shown that cotton straw can be processed and used as roughage for ruminants. Compared with wheat straw and corn straw, cotton straw has the highest crude protein and cellulose content. After processing, cotton straw is combined with other feeds as animal feed to ensure the supply of nutrition, enable animals to play a normal production performance, and reduce feed costs. At present, the main methods for processing crop straw are physical, chemical, and biological methods. Compared with physical and chemical methods, after cotton straw is treated and fermented by microorganisms, the product has a sour and fragrant smell, soft texture, high nutritional value, and high animal digestibility. During the fermentation process, in an anaerobic environment with suitable temperature, the microorganisms have strong reproduction and increased secretion enzyme production. The cellulose xylan chain and lignin polymer ester chain in the straw are degraded by enzymes, and the increased expansion of the straw allows rumen microorganisms to directly contact cellulose, which can improve the crude fiber digestibility of animals. However, cotton straw contains too much crude protein and cellulose, which makes it difficult to ferment alone. A large amount of nutrients needs to be added, which greatly increases the fermentation cost. The fermentation cost is even higher than its utilization value. Summary of the invention

[0004] The purpose of the present invention is to provide a method for preparing cotton straw fermented feed in a cucurbit seed production method, which can solve the problems of low inoculation amount of zucchini pulp fermentation, weak strain activity, mildew on the fermentation surface of zucchini pulp, and unstable biological nutritional indicators of zucchini pulp after fermentation.

[0005] In one aspect of the present invention, the present invention provides a method for preparing a cotton straw fermented feed in a gourd seed production method. According to an embodiment of the present invention, the method comprises the following steps:

[0006] (1) Propagate the strain under suitable conditions without foreign bacteria to obtain a high-density rejuvenated bacterial solution;

[0007] (2) After beating and crushing the zucchini pulp for seed, adding high-density rejuvenation bacterial liquid and enzyme preparation, mixing evenly, and performing anaerobic fermentation to obtain zucchini pulp fermentation liquid for seed;

[0008] (3) Grinding cotton straw into powder, adding seed zucchini pulp fermentation liquid and enzyme preparation to mix evenly, and fermenting to obtain cotton straw fermented feed for seed zucchini seed production method.

[0009] In addition, the method for preparing a cotton straw fermented feed for a gourd seed production method according to the above embodiment of the present invention may also have the following additional technical features:

[0010] In some embodiments of the present invention, the high-density rejuvenated bacterial liquid includes Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Enterococcus faecium, and Enterococcus faecalis, and the enzyme preparation includes cellulase, pectinase, xylanase, mannanase, and glucanase.

[0011] In some embodiments of the present invention, the volume ratio of the Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Enterococcus faecium, and Enterococcus faecalis is 30:5-30:3-20:3-20:3-20, and the weight ratio of the cellulase, pectinase, xylanase, mannanase, and glucanase is 10:0.2-100:0.2-100:0.2-100:0.2-100.

[0012] In some embodiments of the present invention, the preparation method of the high-density rejuvenation bacterial liquid culture is as follows: adding culture medium into a container and sterilizing it, and then inoculating and culturing it.

[0013] In some embodiments of the present invention, the culture medium includes the following components: 25-30 g / L glucose, 8-10 g / L corn steep liquor powder, 3-5 g / L beef extract, 5-10 g / L yeast extract, 3-5 g / L sodium chloride, 2-3 g / L potassium dihydrogen phosphate, 0.6-0.8 g / L magnesium sulfate, and 0.25-0.3 g / L manganese sulfate.

[0014] In some embodiments of the present invention, the culture is aerobic culture with ventilation, a container speed of 80-300 r / min, a ventilation volume of 0.2-2VVM, a tank pressure of 0.02-0.1 MPa, a temperature of 35-37° C., and a culture time of 16-2 h.

[0015] In some embodiments of the present invention, in step (2), the weight ratio of the seed zucchini pulp, the high-density rejuvenation bacterial solution and the enzyme preparation is 100:0.1-300:0-10.

[0016] In some embodiments of the present invention, in step (2), the temperature of anaerobic fermentation is 0 to 50° C., and the fermentation time is 1 to 300 days.

[0017] In some embodiments of the present invention, in step (3), the cotton stalks are crushed into 20-60 mesh powder, and the weight ratio of the cotton stalk powder, the zucchini pulp fermentation liquid for seeds and the enzyme preparation is 100:1-80:0-10.

[0018] In some embodiments of the present invention, in step (3), the fermentation temperature is 0 to 50° C., and the fermentation time is 3 to 10 days.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1) The cotton straw fermented feed for the gourd seed production method of the present invention is rich in soluble fiber polysaccharides, soluble proteins, active small peptides, organic acids, probiotics, biological enzymes and other biologically active nutrients, which improves the palatability of straw feed and has the characteristics of high feed intake, easy digestion and absorption, good growth-promoting performance and the like.

[0021] 2) The present invention shortens the fermentation period of the seed squash pulp, reduces the frequency of bacterial contamination during fermentation, and reduces the fluctuation range of biological nutritional indicators of the fermentation product; utilizes a large amount of live bacteria and biological enzymes produced in the fermentation liquid of the seed squash pulp to accelerate the decomposition rate of anti-nutritional factors such as cellulose and xylan chains in the cotton stalks, and increases the soluble content; not only the digestibility of crude fiber and crude protein in the cotton stalk is improved, but also the product has the characteristics of storage resistance and good animal palatability, thereby realizing the comprehensive utilization of the seed squash pulp and cotton stalks.

[0022] 3) The present invention utilizes a large amount of live bacteria and biological enzymes produced in the fermentation liquid of the seed squash pulp to solve the problems of slow decomposition, low decomposition rate and poor feed palatability of anti-nutritional factors such as cellulose and xylan chains in cotton straw; at the same time, it also solves the problem of high water content in the single fermentation of the seed squash pulp and large loss in direct feeding.

[0023] 4) The present invention ingeniously utilizes the gourd juice for anaerobic fermentation, utilizes the facultative anaerobic characteristics of brewer's yeast, rapidly consumes the residual oxygen in the material mixture, and ferments to produce ethanol and carbon dioxide, which covers the surface of the gourd fermentation material, and can effectively inhibit the growth of mold.

[0024] 5) The fermented feed of the present invention does not need to add other nutrients, and can be fermented only with zucchini pulp and cotton stalks as raw materials, which greatly reduces the cost and improves the utilization value of zucchini pulp and cotton stalks. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Example 1

[0027] A method for preparing cotton straw fermented feed in a gourd seed production method comprises the following steps:

[0028] (1) Preparation of high-density rejuvenating bacterial solution:

[0029] Add 3000g of glucose, 1000g of corn steep liquor powder, 500g of beef extract, 1000g of yeast extract, 500g of sodium chloride, 200g of potassium dihydrogen phosphate, 60g of magnesium sulfate, and 25g of manganese sulfate to the seed tank, add water to 140L, sterilize at 121°C for 30 minutes, cool to the culture temperature, adjust the pH to 7.0, inoculate the shake flask strains (50kg of saccharomyces cerevisiae, 30kg of Bacillus subtilis, 30kg of Bacillus licheniformis, 20kg of Enterococcus faecium, and 20kg of Enterococcus faecalis) for ventilation and aerobic culture, the seed tank speed is 200r / min, the ventilation volume is 0.8VVM, the tank pressure is 0.05Mpa, the temperature is 37°C, and the culture is carried out for 20 hours to obtain 150kg of high-density rejuvenation bacterial liquid. Among them, the condensed water produced after steam sterilization increases the weight by 10 kg.

[0030] (2) Preparation of seeds from zucchini pulp fermentation liquid:

[0031] After 1350 kg of seed zucchini pulp was pulped and crushed, 150 kg of high-density rejuvenating bacteria liquid, 300 g of cellulase, 300 g of pectinase, 150 g of xylanase, 150 g of mannanase, and 150 g of glucanase were added in sequence and mixed evenly, and then anaerobically fermented at 37°C for 3 days to obtain 1494.4 kg of seed zucchini pulp fermentation liquid, of which 6.6 kg was lost during fermentation.

[0032] (3) Preparation of cotton straw fermented feed by gourd seed production method:

[0033] 3300kg cotton straw was crushed into 20 mesh powder, 1494.4kg zucchini pulp fermentation liquid, 1500g cellulase, 500g pectinase, 1200g xylanase, 1200g mannanase, 1200g glucanase were added in sequence and mixed evenly, and then anaerobically fermented at 37℃ for 3 days to obtain 4724.7kg zucchini seed production cotton straw fermented feed, of which 75.3kg was lost in fermentation.

[0034] Example 2

[0035] A method for preparing cotton straw fermented feed in a gourd seed production method comprises the following steps:

[0036] (1) Preparation of high-density rejuvenating bacterial solution:

[0037] Add 2000g of glucose, 667g of corn steep powder, 375g of beef extract, 667g of yeast extract, 375g of sodium chloride, 13.3g of potassium dihydrogen phosphate, 40g of magnesium sulfate, and 16.7g of manganese sulfate to the seed tank, add water to 92L, sterilize at 121°C for 30 minutes, cool to the culture temperature, adjust the pH to 7.0, inoculate the shake flask strains (20kg of saccharomyces cerevisiae, 20kg of Bacillus subtilis, 30kg of Bacillus licheniformis, 20kg of Enterococcus faecium, and 20kg of Enterococcus faecalis) for ventilation and aerobic culture, the seed tank speed is 200r / min, the ventilation volume is 0.8VVM, the tank pressure is 0.05Mpa, the temperature is 37°C, and the culture is carried out for 20 hours to obtain 100kg of high-density rejuvenation bacterial liquid. Among them, the condensed water produced after steam sterilization increases the weight by 8 kg.

[0038] (2) Preparation of seeds from zucchini pulp fermentation liquid:

[0039] After 900 kg of seed-use zucchini pulp was pulped and crushed, 100 kg of high-density rejuvenation bacteria liquid, 300 g of cellulase, 300 g of pectinase, 150 g of xylanase, 150 g of mannanase, and 150 g of glucanase were added in sequence and mixed evenly, and then anaerobically fermented at 37°C for 3 days to obtain 996.5 kg of seed-use zucchini pulp fermentation liquid, of which 3.5 kg was lost during fermentation.

[0040] (3) Preparation of cotton straw fermented feed by gourd seed production method:

[0041] 2500kg cotton straw was crushed into 20 mesh powder, 996.5kg zucchini pulp fermentation liquid, 1170g cellulase, 400g pectinase, 910g xylanase, 910g mannanase, 910g glucanase were added in sequence and mixed evenly, and then anaerobically fermented at 37℃ for 5 days to obtain 3450.3kg zucchini seed production cotton straw fermented feed, of which 50.7kg was lost due to fermentation.

[0042] Example 3

[0043] A method for preparing cotton straw fermented feed in a gourd seed production method comprises the following steps:

[0044] (1) Preparation of high-density rejuvenating bacterial solution:

[0045] Add 2000g of glucose, 667g of corn steep powder, 375g of beef extract, 667g of yeast extract, 375g of sodium chloride, 13.3g of potassium dihydrogen phosphate, 40g of magnesium sulfate, and 16.7g of manganese sulfate to the seed tank, add water to 92L, sterilize at 121°C for 30 minutes, cool to the culture temperature, adjust the pH to 7.0, inoculate the shake flask strains (50kg of saccharomyces cerevisiae, 15kg of Bacillus subtilis, 15kg of Bacillus licheniformis, 15kg of Enterococcus faecium, and 5kg of Enterococcus faecalis) for aeration and aerobic culture, the seed tank speed is 200r / min, the ventilation volume is 0.8VVM, the tank pressure is 0.05Mpa, the temperature is 37°C, and the culture is carried out for 20 hours to obtain 100kg of high-density rejuvenation bacterial liquid. Among them, the condensed water produced after steam sterilization increases the weight by 8 kg.

[0046] (2) Preparation of seeds from zucchini pulp fermentation liquid:

[0047] After 900 kg of seed-use zucchini pulp was pulped and crushed, 100 kg of high-density rejuvenating bacteria liquid, 300 g of cellulase, 300 g of pectinase, 150 g of xylanase, 150 g of mannanase, and 100 g of glucanase were added in sequence and mixed evenly, and then anaerobically fermented at 37°C for 3 days to obtain 993.2 kg of seed-use zucchini pulp fermentation liquid, of which 6.8 kg was lost during fermentation.

[0048] (3) Preparation of cotton straw fermented feed by gourd seed production method:

[0049] 2500kg cotton straw was crushed into 20 mesh powder, 993.2kg zucchini pulp fermentation liquid, 1200g cellulase, 800g pectinase, 1000g xylanase, 1000g mannanase, 1000g glucanase were added in sequence and mixed evenly, and then anaerobically fermented at 37℃ for 7 days to obtain 3407.2kg zucchini seed production cotton straw fermented feed, of which 91kg was lost during fermentation.

[0050] Comparative Example 1

[0051] A method for preparing cotton straw fermented feed in a gourd seed production method comprises the following steps:

[0052] 250 kg of cotton stalks were crushed into 20-mesh powder, 90 kg of zucchini pulp for seed, 0.6 kg of the same amount of enzyme preparation as in Example 3, and 10 kg of high-density rejuvenation bacteria liquid with the same proportion, and then uniformly mixed to obtain 350.6 kg of cotton stalk fermentation wet base.

[0053] 350.6 kg of cotton stalks were fermented wet base at 37°C for 7 days to obtain 349.5 kg of cotton stalks fermented feed for seed gourd production method, of which 1.1 kg was lost during fermentation.

[0054] Comparative Example 2

[0055] A method for preparing cotton straw fermented feed in a gourd seed production method comprises the following steps:

[0056] 90 kg of seed zucchini pulp was pulped and crushed, and then 5 kg of saccharomyces cerevisiae liquid, 1.5 kg of Bacillus subtilis liquid, 1.5 kg of Bacillus licheniformis liquid, 1.5 kg of Enterococcus faecium liquid, 0.5 kg of Enterococcus faecalis liquid, 30 g of cellulase: 30 g of pectinase: 15 g of xylanase: 15 g of mannanase: 10 g of glucanase sold on the market were mixed evenly, and anaerobically fermented at 37°C for 3 days to obtain 99.8 kg of similar seed zucchini pulp fermentation liquid, of which 0.3 kg was lost during fermentation.

[0057] 250 kg of cotton straw was crushed into 20 mesh powder, 99.8 kg of zucchini pulp fermentation liquid for seed, 120 g of cellulase, 80 g of pectinase, 100 g of xylanase, 100 g of mannanase and 100 g of glucanase were added in sequence and mixed evenly, and then anaerobically fermented at 32-47 ° C for 7 days to obtain 346.6 kg of similar zucchini pulp seed production method cotton straw fermented feed, of which 3.7 kg was lost during fermentation.

[0058] The cotton straw fermented feed of the cucurbit seed production method of Example 3 was compared with the fermented products of Comparative Examples 1-2.

[0059] The contents of main active substances in the fermented feeds prepared in Example 3 and Comparative Examples 1-2 are shown in Table 1 below.

[0060] Table 1 Nutrient content of fermented feed prepared in Example 3 and Comparative Examples 1-2

[0061] project Comparative Example 1 Comparative Example 2 Example 3 Color and shape Yellow green solid Brown solid Brown yellow solid Material odor Wine aroma Wine aroma Wine aroma Moisture / % ≤41.8 ≤42 ≤44 Crude protein / % ≥5.6 ≥5.6 ≥5.6 Soluble protein / wt% ≥1.45 ≥1.87 ≥3.65 Soluble fiber / wt% ≥7.0 ≥7.2 ≥7.7 Lactic acid content / wt% ≥0.2 ≥1.0 ≥3.0 Organic acid content / wt% ≥0.5 ≥1.4 ≥3.5 Total calcium / wt% ≥0.65 ≥0.65 ≥0.65 Total phosphorus / wt% ≥0.2 ≥0.2 ≥0.2 Probiotic content / cfu / g <![CDATA[≥0.9×10 9 ]]> <![CDATA[≥2×10 9 ]]> <![CDATA[≥7×10 9 ]]>

[0062] Comparison of the feeding effects of the fermented feed prepared in Example 3 and Comparative Examples 1-2 on beef cattle:

[0063] Three groups of feeds were set up in the experiment: the feed of the control group 1 was the fermented feed added with comparative example 1, the feed of the control group 2 was the fermented feed added with comparative example 2, and the feed of the experimental group was the fermented feed added with example 3.

[0064] Experimental animals: 60 healthy Simmental beef cattle weighing 120 kg were selected as experimental animals. They were paired and divided into 3 groups according to the principle of similar age and weight, with 20 in each group.

[0065] Feeding method: The weight of fermented feed in the diet of the two groups of beef cattle fed with fermented feed during the pre-trial period and the formal period was calculated based on the feed with a water content of 13wt%. The concentrated feed and roughage fed were mixed evenly in a 1:1 ratio. During the trial, the beef cattle were free to eat and drink water throughout the day. The trial period was 90 days and the pre-feeding period was 3 days.

[0066] Experimental management: Before the preliminary test, the cowshed was repaired and disinfected to reduce the impact of environmental hygiene and parasites on the nutritional metabolism of cattle. After random grouping, the cattle were numbered uniformly and raised in pens according to the groups. They were allowed to eat freely and provided with sufficient clean drinking water in the cowshed. The sanitation and epidemic prevention conditions and environmental conditions of the pens were also ensured to be good.

[0067] Experimental results and analysis: The growth performance of the three groups of Simmental beef cattle is shown in Table 2.

[0068] Table 2 Comparison of growth performance of three groups of Simmental beef cattle

[0069] Experimental Project Control group 1 Control group 2 Experimental Group Number of test heads 50 50 50 Number of days for formal test / d 30 30 30 Initial total weight / kg 5905.6 5904.9 5905.7 Final weight of experiment / kg 8836.5 9111 9252 Total weight gain / kg 2930.9 3206.1 3346.3 Total material consumption / kg 13920 14250 14700 Average daily weight gain per beef cattle / kg 1.63 1.78 1.86 Average feed consumption per beef cattle per day / kg 7.73 7.9 8.17 Diarrhea rate / % 12 4 2 Feed to meat ratio 4.75:1 4.44:1 4.39:1

[0070] As can be seen from Table 2, the daily weight gain of the Simmental beef cattle group in the test group was increased by 14.17% and 4.37% respectively compared with the Simmental beef cattle groups in the control group 1 and the control group 2; the feed intake was increased by 5.60% and 3.16% respectively, the diarrhea rate was reduced by 83.33% and 50% respectively; the feed-to-meat ratio was reduced by 1.16% and 7.5% respectively. The results show that the fermented feed prepared in Example 3 can significantly improve the production performance of Simmental beef cattle.

[0071] Comparative Example 3

[0072] A method for preparing a single fermented gourd feed comprises the following steps:

[0073] 340 kg of zucchini pulp for seed use, 0.6 kg of the enzyme preparation of step 3 in Example 3 in the same proportion, and 10 kg of high-density rejuvenating bacterial liquid in the same proportion were uniformly mixed, and anaerobically fermented at 37° C. for 3 days to obtain 346 kg of zucchini pulp fermentation liquid for seed use, wherein 4.6 kg was lost during fermentation.

[0074] Comparative Example 4

[0075] The method for preparing cotton straw fermented feed comprises the following steps:

[0076] 240 kg of cotton straw was crushed into 20 mesh powder, 25 kg of beet molasses, 1 kg of yeast extract, 0.35 kg of magnesium sulfate, 0.7 kg of potassium dihydrogen phosphate, 0.35 kg of manganese sulfate, and 63 kg of clean water were added, and the mixture was uniformly mixed to form a wet base. Then, 0.6 kg of the enzyme preparation with the same proportion in step (3) of Example 3 and 10 kg of the high-density rejuvenation bacteria liquid with the same proportion were added and uniformly mixed. The mixture was anaerobically fermented at 37° C. for 7 days to obtain 348 kg of cotton straw fermented feed. Among them, 2.7 kg was lost during fermentation.

[0077] Table 3 Comparison of fermentation costs between Example 3 and Comparative Examples 3-4

[0078] Experimental Project Example 3 Comparative Example 3 Comparative Example 4 Fermentation material wet basis moisture 44％ 92.20％ 43.50％ Fermentation material wet basis to dry basis weight / ton 0.56 0.078 0.565 Cost of wet-based enzyme preparation for fermentation material / yuan / ton 36.5 54.2 53.9 Fermentation material wet base bacteria + auxiliary materials cost / yuan / ton 31.7 28.9 307.4 Fermentation material wet-base packaging bag quantity / piece / ton 40 40 40 Packaging bag price / yuan / piece 2.50 2.50 2.50 Packaging cost: Yuan / ton 100.0 100.0 100.0 Transportation cost of wet-based fermentation material: Yuan / ton 100.0 100.0 100.0 Total wet-base cost of fermentation material: Yuan / ton 268.2 283.1 561.3

[0079] It can be concluded from the above table that the processing cost of the fermented feed produced in Comparative Examples 3 and 4, which is mixed with the gourd and cotton stalks in the same proportion as in Example 3 to prepare the gourd and cotton stalk fermented mixed feed wet base, is: (900×283.1+2500×561.3)÷3400=487.6 yuan / ton, which is 81.8% higher than the gourd and cotton stalk fermented feed wet base produced in Example 3, i.e., 219.4 yuan / ton. Taking the processing of 1 million tons of gourd and cotton stalk fermented feed wet base as an example, the cost saving can be 1 million tons×219.4 yuan / ton=219.4 million yuan, with significant economic benefits.

[0080] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the structure of the present invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A method for preparing a cotton straw fermented feed by a gourd seed production method, characterized in that: The following steps are involved: (1) Propagate the strain under suitable conditions without foreign bacteria to obtain a high-density rejuvenated bacterial solution; (2) After beating and crushing the zucchini pulp for seed, adding high-density rejuvenation bacterial liquid and enzyme preparation, mixing evenly, and performing anaerobic fermentation to obtain zucchini pulp fermentation liquid for seed; (3) Grinding cotton straw into powder, adding seed zucchini pulp fermentation liquid and enzyme preparation to mix evenly, and fermenting to obtain cotton straw fermented feed for seed zucchini seed production method.

2. The method for preparing a cotton straw fermented feed by a gourd seed production method according to claim 1, characterized in that: The high-density rejuvenated bacterial liquid comprises saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, enterococcus faecium and enterococcus faecalis, and the enzyme preparation comprises cellulase, pectinase, xylanase, mannanase and glucanase.

3. The method for preparing a cotton straw fermented feed for gourd seed production method according to claim 2, characterized in that: The volume ratio of the saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, enterococcus faecium and enterococcus faecalis is 30:5-30:3-20:3-20:3-20, and the weight ratio of the cellulase, pectinase, xylanase, mannanase and glucanase is 10:0.2-100:0.2-100:0.2-100:0.2-100.

4. The method for preparing a cotton straw fermented feed by a gourd seed production method according to claim 1 or 2, characterized in that: The preparation method of the high-density rejuvenation bacterial liquid culture is as follows: adding a culture medium into a container, sterilizing it, and then inoculating and culturing it.

5. The method for preparing a cotton straw fermented feed for gourd seed production method according to claim 3, characterized in that: The culture medium comprises the following components: 25-30 g / L glucose, 8-10 g / L corn steep liquor powder, 3-5 g / L beef extract, 5-10 g / L yeast extract, 3-5 g / L sodium chloride, 2-3 g / L potassium dihydrogen phosphate, 0.6-0.8 g / L magnesium sulfate and 0.25-0.3 g / L manganese sulfate.

6. The method for preparing a cotton straw fermented feed in a gourd seed production method according to claim 3, characterized in that: The culture is aerobic culture with ventilation, a container rotation speed of 80-300 r / min, a ventilation volume of 0.2-2 VVM, a tank pressure of 0.02-0.1 MPa, a temperature of 35-37° C., and a culture time of 16-2 h.

7. The method for preparing a cotton straw fermented feed for gourd seed production method according to claim 1, characterized in that: In the step (2), the weight ratio of the seed zucchini pulp, the high-density rejuvenation bacterial solution and the enzyme preparation is 100:0.1-300:0-10.

8. The method for preparing a cotton straw fermented feed in a gourd seed production method according to claim 1, characterized in that: In the step (2), the temperature of the anaerobic fermentation is 0 to 50° C., and the fermentation time is 1 to 300 days.

9. The method for preparing a cotton straw fermented feed in a gourd seed production method according to claim 1, characterized in that: In the step (3), the cotton stalks are crushed into 20-60 mesh powder, and the weight ratio of the cotton stalk powder, the zucchini pulp fermentation liquid for seeds and the enzyme preparation is 100:1-80:0-10.

10. The method for preparing a cotton straw fermented feed in a gourd seed production method according to claim 1, characterized in that: In the step (3), the fermentation temperature is 0 to 50° C. and the fermentation time is 3 to 10 days.