Traditional Chinese medicine composition for blackening and developing hair as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention belongs to the technical field of cosmetics, and provides a traditional Chinese medicine composition for blackening and developing hair as well as a preparation method and application thereof, and the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 3-6 parts of polygonum multiflorum, 3-6 parts of angelica sinensis, 2-5 parts of oroxylum indicum and 3-5 parts of lycium ruthenicum. The composition disclosed by the invention has the effects of alleviating the growth environment state of hair follicles and promoting melanin cells to generate melanin, and is easier to absorb by scalp by adopting a nanotechnology. The preparation method of the traditional Chinese medicine composition is simple and convenient, and large-scale production and application are facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a traditional Chinese medicine composition for blackening and growing hair, and a preparation method and application thereof. Background Art

[0002] Gray hair, also known as grey hair or white hair, is a common physiological phenomenon that is usually caused by a decrease in melanin in the body. Melanin is a pigment produced in hair follicles that gives hair its natural color. As we age, the amount of melanin in the body gradually decreases, causing the hair to turn gray. In addition to age factors, genetics also affect the appearance of gray hair to a certain extent.

[0003] Although gray hair is somewhat inevitable, there are ways to slow its appearance or treat gray hair that has already appeared.

[0004] Chinese patent CN105853276A discloses a natural plant formula for black hair and delaying gray hair, which is made from 14 parts of processed Polygonum multiflorum, 2 parts of black sesame, 10 parts of mulberry, 8 parts of angelica, 5 parts of Platycladus orientalis, 7 parts of Ecliptae, 6 parts of Platycladus orientalis seeds, 4 parts of mint, 8 parts of Scutellaria baicalensis, 16 parts of Cuscuta australis, 2 parts of Angelica dahurica, 4 parts of Sargassum, 9 parts of Salvia miltiorrhiza, 9 parts of Ligustrum lucidum, 16 parts of stir-fried Vitex rotundifolia, 10 parts of Lycium barbarum, 5 parts of Astragalus, 3 parts of Smilax glabra, and 15 parts of Ginseng. This method has many raw materials, a complicated preparation process, and has a black hair effect but no hair growth effect.

[0005] Chinese patent CN106977997A discloses a disease prevention and health care functional nano coating, which uses a secret recipe for hair-blackening Chinese medicine nano powder, and the Chinese medicine ingredients: Morinda officinalis, Cistanche deserticola, Asparagus cochinchinensis, Schisandra chinensis, Platycladus orientalis, Achyranthes bidentata, Cuscuta australis, Polygala tenuifolia, Dendrobium candidum, Dioscorea opposita, Saposhnikovia divaricata, White Poria cocos, Ginseng, Rehmannia glutinosa, Rubus idaeus, Rhizoma Dioscoreae, Radix Dioscoreae, Acanthopanax acanthopanax, Tianxiong, Chuanduan, Photinia serrulata, Eucommia ulmoides, Cnidium monnieri, Aquilaria sinensis, etc. are nano-pulverized. This method has a complex preparation process, high raw material cost, and a general hair-blackening and hair-growing effect. Summary of the invention

[0006] Aiming at the technical problems of complicated preparation process and general hair blackening and growth effect in the prior art, the present invention provides a traditional Chinese medicine composition for hair blackening and growth, and a preparation method and application thereof.

[0007] The technical solution adopted by the present invention is:

[0008] In a first aspect, the present invention provides a traditional Chinese medicine composition for blackening and growing hair.

[0009] Specifically, a Chinese medicine composition for hair growth and development includes Polygonum multiflorum, Angelica sinensis, Oroxylum indica and Black Wolfberry;

[0010] Specifically, the Chinese medicine composition comprises the following raw materials in parts by weight: 3-6 parts of Polygonum multiflorum, 3-6 parts of Angelica sinensis, 2-5 parts of Oroxylum indica and 3-5 parts of Lycium barbarum;

[0011] Specifically, the Chinese medicine composition comprises one or more of the following raw materials by weight: 2-4 parts of Ligustrum lucidum fruit, 3-5 parts of ginseng, 3-5 parts of Platycladus orientalis leaves and 3-4 parts of mint;

[0012] The Chinese medicine composition for hair growth and development includes Polygonum multiflorum, Chinese angelica, Oroxylum indica, Lycium barbarum, Ligustrum lucidum, Ginseng root, Platycladus orientalis leaf and mint;

[0013] The Chinese medicine composition is composed of the following raw materials in weight fractions: including the following raw materials in weight fractions: 3-6 parts of Polygonum multiflorum, 3-6 parts of Angelica sinensis, 2-5 parts of Oroxylum indica, 3-5 parts of Lycium barbarum, 2-4 parts of Ligustrum lucidum, 3-5 parts of Ginseng, 3-5 parts of Platycladus orientalis leaves and 3-4 parts of Mint;

[0014] Preferably, the Chinese medicine composition is composed of the following raw materials by weight: 5 parts of Polygonum multiflorum, 5 parts of Angelica sinensis, 4 parts of Oroxylum indica, 5 parts of Lycium barbarum, 3 parts of Ligustrum lucidum, 4 parts of Ginseng, 5 parts of Platycladus orientalis leaves and 3 parts of Mint;

[0015] Specifically, the angelica root can be selected from angelica root and angelica leaf;

[0016] Preferably, the angelica is angelica root;

[0017] Specifically, the oroxylum butterfly can be selected from oroxylum butterfly flowers, oroxylum butterfly roots and oroxylum butterfly bark;

[0018] Preferably, the Oroxylum butterfly is an Oroxylum butterfly root;

[0019] Specifically, the raw materials of the Chinese medicine composition are in the form of Chinese herbal medicine plants and / or Chinese herbal medicine extracts.

[0020] The effects of the components of the Chinese medicine composition for blackening and growing hair of the present invention are as follows:

[0021] Polygonum multiflorum is rich in nutrients, such as amino acids, vitamins and minerals, which can promote the nutrition of hair follicles and enhance the growth ability of hair, thereby effectively promoting hair growth; it can nourish hair, supplement the nutrients needed for hair, improve hair dryness, frizziness and other problems, and make hair softer, smoother and more elastic; in addition, Polygonum multiflorum contains antioxidants, which can help protect hair from damage by free radicals, slow down the aging process of hair, and keep hair healthy, and it can also stimulate the production of melanin, help delay the process of hair graying, and maintain black hair color.

[0022] Angelica root has a certain effect on dark hair. It contains active ingredients that can promote blood circulation in the hair follicles in the scalp, increase the nutrient supply to the hair follicles, and help promote hair growth. In addition, it is rich in vitamins and minerals that can provide nutrition to the hair, enhance the health and elasticity of the hair, and improve the hair quality. In addition, angelica root has antioxidant and anti-inflammatory effects, which can reduce hair loss and keep hair healthy.

[0023] The main components of Oroxylum indica are flavonoids, which have certain effects in anti-inflammatory, anti-allergic and other aspects.

[0024] Black wolfberry has multiple benefits for dark hair. First, it is rich in nutrients that can promote the growth and development of hair follicles, making hair thicker. Second, black wolfberry has antioxidant, anti-inflammatory and anti-aging effects, which can help reduce hair loss.

[0025] Ligustrum lucidum is rich in vitamins C and E, which can promote hair growth and enhance hair health. Its active ingredients also have antioxidant and anti-inflammatory effects, which help reduce hair loss and keep hair healthy. In addition, Ligustrum lucidum can improve scalp conditions, reduce dandruff and scalp inflammation, and promote hair growth and dark hair.

[0026] Ginseng has a variety of benefits for hair health. It is rich in nutrients such as ginsenosides, ginsenosides and ginsenosides, which help nourish the hair and scalp, promote hair growth and strengthen hair. It also has antioxidant and anti-inflammatory properties, which help reduce hair damage and inflammation, protecting hair from external damage. In addition, ginseng can also promote blood circulation in the scalp and improve scalp health, which can help prevent hair loss problems.

[0027] Platycladus orientalis leaves have the effects of cooling blood and stopping bleeding, removing phlegm and relieving cough, and promoting hair growth and darkening hair. Studies have found that the resin components in Platycladus orientalis leaves have a significant activation effect on TYR, which is the effective ingredient for its hair darkening effect. In addition, the petroleum ether part of Platycladus orientalis leaves has the highest activation rate for TYR, and is related to its concentration. High concentration has the effect of upregulating TYR activity.

[0028] The main effects of mint include dispelling wind-heat, clearing the head and eyes, promoting qi circulation, soothing the liver, relieving sore throat, and clearing rashes. Its mechanism of action on black hair is mainly reflected in its ability to increase TYR activity, upregulate TYR mRNA levels, and promote melanocyte proliferation.

[0029] In a second aspect, the present invention provides a method for preparing a Chinese medicine composition for blackening and growing hair, the preparation method comprising the following steps:

[0030] (1) The raw material and water are mixed in a mass ratio of 1:3, heated at 90-110° C., and then laid and allowed to stand to obtain a sample solution;

[0031] (2) placing the sample solution obtained in step (1) in a vacuum microwave apparatus and heating it at 75-95° C. for 160-200 s to obtain a microwave pretreated sample;

[0032] (3) stirring and dissolving the microwave pretreated sample obtained in step (2) in 100 mL of ultrapure water to prepare a nano spray drying preparatory solution;

[0033] (4) Atomizing the nano spray drying preliminary solution obtained in step (3) by a nano spray dryer, and drying it in a spray drying cylinder at 60-80° C. to form nanoparticles.

[0034] Specifically, in step (1), when the raw material is in the form of a Chinese herbal medicine, it needs to be crushed;

[0035] The water in step (1) can be selected from distilled water and ultrapure water;

[0036] The vacuum microwave equipment in step (2) has a vacuum degree of 70 KPa.

[0037] In a third aspect, the present invention provides a use of a traditional Chinese medicine composition for blackening and growing hair in preparing cosmetics for blackening and growing hair.

[0038] In a fourth aspect, the present invention provides a cosmetic for blackening and growing hair;

[0039] Specifically, the cosmetics include a Chinese medicine composition and excipients acceptable in cosmetics;

[0040] Specifically, the cosmetic is a product used on the hair and / or scalp;

[0041] The mass percentage of the Chinese medicine composition in the cosmetic is 0.01-10%;

[0042] Preferably, the mass percentage of the traditional Chinese medicine composition in the cosmetic is 0.01-2%;

[0043] The auxiliary materials include one or more of emulsifiers, thickeners, humectants, oils, chelating agents, and preservatives.

[0044] The emulsifier comprises one or more of organic olive oil emulsifying wax, self-emulsifying monoglyceride, methyl glucoside sesquistearate and glyceryl monostearate; and / or

[0045] The thickener comprises one or more of carbomer, polyacrylate cross-linked polymer and hydroxyethyl cellulose; and / or

[0046] The moisturizing agent comprises one or more of glycerin, propylene glycol and sodium hyaluronate; and / or

[0047] The oil comprises one or more of caprylic triglyceride, capric triglyceride, shea butter, isohexadecane, isopropyl palmitate, dimethicone and cyclopentasiloxane; and / or

[0048] The chelating agent comprises disodium edetate; and / or

[0049] The preservatives include one or more of phenoxyethanol, methylparaben, propylparaben and sodium benzoate.

[0050] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0051] (1) The hair darkening and growth composition provided by the present invention comprises a variety of plant ingredients, which are highly active, less irritating, and can effectively nourish hair follicles, activate tyrosinase, and promote the growth of melanocytes;

[0052] (2) The preparation method of the hair darkening and growth composition of the present invention is simple and convenient, and is conducive to large-scale production and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 The results are shown in Table 3. The results are shown in Table 3. The results are shown in Table 3. The results are shown in Table 3. The results are shown in Table 3. The results are shown in Table 3.

[0054] Figure 2 The effect of the composition of Example 3 on the melanin content of B16 melanoma cells, wherein ns means no statistical significance, * means p<0.05, there is a statistical difference; ** means p<0.01, there is a statistical difference; *** means p<0.001, there is a statistical difference; **** means p<0.0001, there is a statistical difference;

[0055] Figure 3 The effect of the composition of Example 3 on the tyrosinase activity of B16 melanoma cells, wherein ns means no statistical significance, * means p<0.05, there is a statistical difference; ** means p<0.01, there is a statistical difference; *** means p<0.001, there is a statistical difference; **** means p<0.0001, there is a statistical difference;

[0056] Figure 4 This is a photo of Volunteer 1's hair before using the composition;

[0057] Figure 5 This is a photo of Volunteer 1's hair after using the composition;

[0058] Figure 6The photo of volunteer 2's hair before using the composition;

[0059] Figure 7 Photograph of volunteer 2's hair after using the composition. DETAILED DESCRIPTION

[0060] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention. The materials, reagents, etc. used are reagents and materials available from commercial sources unless otherwise specified.

[0061] Source of raw materials: Guangzhou Baizhitang Nanobiotechnology Co., Ltd.

[0062] 1. Example, comparative example setting

[0063] Preparation method of Chinese medicinal composition for blackening and growing hair

[0064] The raw materials were crushed, mixed with ultrapure water at a material-liquid ratio of 1:3, and stirred at 100°C for 60 minutes, stirring while adding. The solution was allowed to stand for a period of time to allow the solution to fully penetrate the cell tissue; the fully infiltrated sample was placed in a vacuum microwave device with a vacuum degree of 70Kpa and heated at 85°C for 180s; the sample after microwave pretreatment was dissolved in 100mL of ultrapure water, stirred and dissolved at 60°C, fully mixed, and configured into a nano spray drying preparatory solution; then the preparatory solution was atomized by a nano spray dryer and dried into nanoparticles at 70°C in a spray drying cylinder.

[0065] Embodiment 1 A Chinese medicine composition for blackening and growing hair

[0066] 3 parts of Polygonum multiflorum, 3 parts of Angelica sinensis roots, 2 parts of Oroxylum indica roots and 3 parts of Lycium barbarum are prepared as described above.

[0067] Embodiment 2 A Chinese medicine composition for blackening and growing hair

[0068] 6 parts of Polygonum multiflorum, 6 parts of Angelica sinensis roots, 5 parts of Oroxylum indica roots and 5 parts of Lycium barbarum are prepared as described above.

[0069] Embodiment 3 A Chinese medicine composition for blackening and growing hair

[0070] 5 parts of Polygonum multiflorum, 5 parts of Angelica sinensis roots, 4 parts of Oroxylum indica roots, 5 parts of Lycium barbarum, 3 parts of Ligustrum lucidum fruits, 4 parts of Ginseng roots, 5 parts of Platycladus orientalis leaves, and 3 parts of Mint, and the preparation method is as above.

[0071] Embodiment 4 A Chinese medicine composition for blackening and growing hair

[0072] 6 parts of Polygonum multiflorum, 6 parts of Angelica sinensis roots, 4 parts of Ligustrum lucidum fruits, 5 parts of Oroxylum indica roots, 5 parts of Ginseng roots, 3 parts of Black Wolfberry, 4 parts of Platycladus orientalis leaves, and 4 parts of Mint, and the preparation method is as above.

[0073] Embodiment 5 A Chinese medicine composition for blackening and growing hair

[0074] 3 parts of Polygonum multiflorum, 3 parts of Angelica sinensis roots, 2 parts of Oroxylum indica roots, 3 parts of Lycium barbarum, 2 parts of Ligustrum lucidum fruits, 3 parts of Ginseng roots, 3 parts of Platycladus orientalis leaves, and 3 parts of Mint, and the preparation method is as above.

[0075] Comparative Example 1

[0076] A single component: Polygonum multiflorum is used, and the preparation method is as mentioned above.

[0077] Comparative Example 2

[0078] A single component: angelica root is used, and the preparation method is as described above.

[0079] Comparative Example 3

[0080] A single component is used: Oroxylum indica root, and the preparation method is as described above.

[0081] Comparative Example 4

[0082] A single component: black wolfberry is used, and the preparation method is as described above.

[0083] Comparative Example 5

[0084] The difference from Example 1 is that it does not contain Polygonum multiflorum, and includes the following raw materials: including the following raw materials: 3 parts of Angelica sinensis roots, 2 parts of Oroxylum indica roots and 3 parts of black wolfberries, and the preparation method is as described above.

[0085] Comparative Example 6

[0086] The difference from Example 1 is that no angelica root is contained, and the following raw materials are included: the following raw materials include: 3 parts of Polygonum multiflorum, 2 parts of Oroxylum indica roots and 3 parts of black wolfberry, and the preparation method is as described above.

[0087] Comparative Example 7

[0088] The difference from Example 1 is that the oroxylum indica root is not contained, and the following raw materials are included: 3 parts of Polygonum multiflorum, 3 parts of Angelica sinensis root and 3 parts of black wolfberry, and the preparation method is as described above.

[0089] Comparative Example 8

[0090] The difference from Example 1 is that black wolfberry is not contained, and the following raw materials are included: 3 parts of Polygonum multiflorum, 3 parts of Angelica sinensis roots and 2 parts of Oroxylum indica roots, and the preparation method is as described above.

[0091] Comparative Example 9

[0092] 2 parts of Polygonum multiflorum, 2 parts of Angelica sinensis root, 1 part of Oroxylum indica root and 2 parts of Lycium barbarum are prepared as described above.

[0093] Comparative Example 10

[0094] 7 parts of Polygonum multiflorum, 7 parts of Angelica sinensis roots, 6 parts of Oroxylum indica roots and 6 parts of Lycium barbarum are prepared as described above.

[0095] Comparative Example 11

[0096] 10 parts of Polygonum multiflorum, 7 parts of Angelica sinensis roots, 6 parts of Oroxylum indica roots, 2 parts of Lycium barbarum, 1 part of Ligustrum lucidum fruit, 2 parts of Ginseng roots, 2 parts of Platycladus orientalis leaves, and 2 parts of Mint, and the preparation method is as above.

[0097] Comparative Example 12

[0098] 2 parts of Polygonum multiflorum, 2 parts of Angelica sinensis roots, 1 part of Oroxylum indica roots, 6 parts of Lycium barbarum, 5 parts of Ligustrum lucidum fruits, 5 parts of Ginseng roots, 6 parts of Platycladus orientalis leaves, and 5 parts of Mint, and the preparation method is as above.

[0099] Comparative Example 13

[0100] Without using nanotechnology, a traditional preparation method is adopted, and the rest is the same as in Example 3.

[0101] Crush the herbs with a grinder, add 75% ethanol and place in an ultrasonic cleaner for 1 hour; use a Buchner funnel to vacuum filter the extract; repeat the operation once and mix the first and second vacuum filtration extracts; place the extract on a rotary evaporator and evaporate it until it becomes a thick paste; pour the concentrated extract into an evaporating dish and place it in an oven to dry into a solid.

[0102] Comparative Example 14

[0103] According to the technical scheme disclosed in Chinese patent CN105853276A, 14 parts of processed Polygonum multiflorum, 2 parts of black sesame, 10 parts of mulberry, 8 parts of angelica, 5 parts of Platycladus orientalis, 7 parts of Ecliptae, 6 parts of Platycladus orientalis seeds, 4 parts of mint, 8 parts of Scutellaria baicalensis, 16 parts of Cuscuta australis, 2 parts of Angelica dahurica, 4 parts of Sargassum, 9 parts of Salvia miltiorrhiza, 9 parts of Ligustrum lucidum, 16 parts of stir-fried Vitex rotundus, 10 parts of Lycium barbarum, 5 parts of Astragalus, 3 parts of Smilax glabra, and 15 parts of Ginseng were taken and ground into powder by a grinder. Crush; weigh 10g of the crushed medicinal materials and put them into a conical flask; add 100ml of 75% ethanol; put it into an ultrasonic cleaner for 1 hour; use a Buchner funnel to vacuum filter the extract; add 100ml of 75% ethanol; put it into an ultrasonic cleaner for 1 hour; use a Buchner funnel to vacuum filter the extract and mix it with the first extract; use a rotary evaporator to evaporate the extract until it becomes a thick paste; pour the concentrated extract into an evaporating dish and put it into an oven to dry into a solid.

[0104] Composition effect test

[0105] Part I: Experimental proof of effect

[0106] Performance test 1: DPPH free radical scavenging rate test

[0107] Free radicals can damage hair follicle cells, affect the normal growth of hair, and cause hair loss and gray hair. Therefore, the free radical scavenging ability of Examples 1-5 and Comparative Examples 1-12 was tested as follows:

[0108] The Chinese medicinal composition for blackening and growing hair prepared in the comparative example of the embodiment was diluted with deionized water to a concentration of 0.25 mg / mL; 100 μL of the sample solution was added to a 96-well plate, and 100 μL of a 0.2 mmol / L DPPH anhydrous ethanol solution was added. After being shaken evenly, it was placed in a dark place at 25° C. to avoid light for reaction for 30 minutes, and then the absorbance A1 of the mixed solution was measured at a wavelength of 517 nm; 100 μL of anhydrous ethanol was taken instead of the DPPH anhydrous ethanol solution to perform the same operation; 100 μL of anhydrous ethanol was taken instead of the sample to perform the same operation; forskolin solution was used as a positive control, 3 parallel experiments were set up for each group to calculate the average value, and the free radical scavenging ability of the Chinese medicinal composition for blackening and growing hair of different groups was calculated according to the DPPH scavenging rate formula.

[0109] DPPH clearance rate = [1-(A1-A2) / A3] × 100%

[0110] Where:

[0111] A1 is the absorbance value of 100μL DPPH working solution + 100μL sample A2 is the absorbance value of 100μL anhydrous ethanol + 100μL sample

[0112] A3 is the absorbance value of 100μL DPPH working solution + 100μL anhydrous ethanol. The test results can be found in Table 1.

[0113] Table 1 Test results of free radical scavenging ability of Examples 1-5 and Comparative Examples 1-14

[0114]

[0115]

[0116] As can be seen from Table 1, the compositions obtained in Examples 1-5 all have good DPPH free radical scavenging ability, with the highest scavenging rate reaching 96.99%, which is much better than that of Comparative Example 14 of the prior art.

[0117] Comparative Examples 1-4 are the effects of a single component, and Comparative Examples 5-8 are the effects of a combination of removing one of Polygonum multiflorum, Angelica sinensis root, Oroxylum indica root, and Black Wolfberry. The free radical scavenging effect of the composition obtained in Examples 1-5 is better than that of Comparative Examples 1-8, indicating that the components have a synergistic effect and are indispensable.

[0118] The free radical scavenging effect of the compositions obtained in Examples 1-5 is better than that of Comparative Examples 9-12. The specific composition ratio of the Chinese medicine composition of the present invention enables Examples 1-5 to have better DPPH free radical scavenging ability.

[0119] Compared with Comparative Example 13 which does not use nanotechnology, Examples 1-5 have excellent DPPH free radical scavenging capabilities.

[0120] Performance Test 2: Tyrosinase Activation Rate

[0121] Prepare 0.5 mg / mL L-tyrosine solution (solvent is phosphate buffer), prepare 35 mg / mL mushroom tyrosinase solution (solvent is phosphate buffer), and use propylene glycol as solvent to prepare 1 mg / mL test sample solution.

[0122] Prepare 4 groups of solutions according to the following proportions:

[0123] (1) 0.7 mL of L-tyrosine solution + 0.7 mL of pure propylene glycol + 0.6 mL of phosphate buffer;

[0124] (2) 0.7 mL pure propylene glycol + 1.3 mL phosphate buffer;

[0125] (3) 0.7 mL of L-tyrosine solution + 0.7 mL of propylene glycol solution of the sample to be tested + 0.6 mL of phosphate buffer;

[0126] (4) 0.7 mL of propylene glycol solution of the sample to be tested + 0.6 mL of phosphate buffer;

[0127] After the above 4 groups of solutions were kept in a 37°C water bath for 10 minutes, 0.3 mL of mushroom tyrosinase solution was added to each, and after reacting for 5 minutes, the solution was quickly transferred to a cuvette, and the absorbance (OD value) was measured at 475 nm. The OD value was recorded and calculated according to the formula. Among them, (OD1-OD2) represents the change in OD value of the reaction solution without an activator, and (OD3-OD4) represents the change in OD value of the reaction solution with an activator (Example 1-5, Comparative Example 1-14 substances). The calculation formula for the tyrosinase activation rate is:

[0128] Tyrosinase activation rate = 100% × [(OD3-OD4)-(OD1-OD2)] / (OD1-OD2)

[0129] The test results can be found in Table 2

[0130] Table 2 Test results of tyrosinase activation rates of Examples 1-5 and Comparative Examples 1-14

[0131] example Tyrosinase activation rate / % Example 1 18.85 Example 2 19.78 Example 3 25.33 Example 4 23.21 Example 5 20.88 Comparative Example 1 12.55 Comparative Example 2 11.33 Comparative Example 3 15.76 Comparative Example 4 10.22 Comparative Example 5 14.33 Comparative Example 6 16.23 Comparative Example 7 15.25 Comparative Example 8 16.21 Comparative Example 9 16.56 Comparative Example 10 17.88 Comparative Example 11 18.23 Comparative Example 12 15.33 Comparative Example 13 16.25 Comparative Example 14 15.23

[0132] As can be seen from Table 2, the compositions obtained in Examples 1-5 all have good tyrosinase activation ability, with the highest activation rate reaching 25%. The tyrosinase activation effect of the compositions obtained in Examples 1-5 is improved compared with the effect of using a single component alone in Comparative Examples 1-4, and is also much improved compared with the effect of removing any component from the compositions in Comparative Examples 5-8. The components have a good synergistic effect, and if any component is missing, the effect is greatly reduced.

[0133] The compositions obtained in Examples 1-5 are superior to those in Example 13 which does not use nanotechnology; the compositions obtained in Examples 1-5 are superior to those in Example 14 of the prior art; the compositions obtained in Examples 1-5 are superior to those obtained in other proportions, and the Chinese medicine compositions of the present invention have better tyrosinase activation ability.

[0134] Part II Cell Viability Test

[0135] The composition of Example 3 was subjected to a cell activity evaluation experiment.

[0136] Example 3 Determination of the activity of the composition on B16 mouse melanoma cells.

[0137] The operation was divided into a blank group, a negative control group and a sample group, with 6 parallel wells in each group, and the process was repeated three times. The blank group was given normal culture medium without cells, the negative control group was given cells and normal culture medium, and the sample group was given cells and the composition of Example 1 at different concentration gradients. The density of B16 cells in good growth state was adjusted to 10 5 / mL, add 100μL / well to a 96-well plate, and culture in a 37℃, 5% CO2 constant temperature incubator for 24h. After the cell monolayer adheres to the wall, prepare the sample solutions of various concentrations, add 100μL / well to a 96-well plate, and culture in a 37℃, 5% CO2 constant temperature incubator for another 24h. Discard the supernatant, add 100μL MTT (0.5mg / mL) to each well, add 100μL DMSO after 4h, and shake on a 37℃ shaker for 10min to dissolve the crystals, check the activity of the cells at 492nm, and express it as cell survival rate. The results are as follows Figure 1 The calculation formula is as follows:

[0138] Cell survival rate = (A1-A2) / (A3-A2)×100%

[0139] Where:

[0140] A1: absorbance value of sample group;

[0141] A2: absorbance value of blank group;

[0142] A3: absorbance value of negative control group.

[0143] By Figure 1 As shown, compared with the blank group, the sample with a concentration of 2.5 mg / mL has a certain toxicity to the cells, and the cell survival rate at a concentration of 1.25 mg / mL and below reaches more than 90%, wherein the cell survival rate at a concentration of 625.00 μg / mL is almost the same as that of the control, and the survival rate is 104.32%, with no significant difference. And as the concentration decreases, the cell survival rate gradually increases, reaching the highest at a concentration of 156.25 μg / mL, with a survival rate of 122.03%, after which the concentration remains stable, indicating that the sample with a concentration of 625.00 μg / mL and below is non-toxic to the cells, and has a certain cell proliferation effect. And when observing the cell morphology, the sample group has no obvious change in cell morphology compared with the control group, and the cells show normal appearance and morphological characteristics. It shows that the composition of the present invention is non-toxic to the scalp and hair follicles at a certain concentration, and also has a certain hair growth effect.

[0144] Example 3 Determination of the effect of the composition on the melanin content of B16 mouse melanoma cells.

[0145] The tyrosinase activity in B16 mouse melanoma cells was measured by NaOH lysis method. The density of B16 melanoma cell suspension was adjusted to 1.8×10 6 Each well has 1 mL of complete medium and is inoculated into a 6-well plate with 1 mL of cell solution per well to make the cell density 3×10 5 / well, placed in an incubator (37℃, 5% carbon dioxide) and cultured for 24h. After the cells adhere, add 100μL of test solution of different concentrations to each well as the sample group (according to the above experimental results, the concentration below 625.00μg / mL is used for operation), and add 1mL of culture medium without the sample to be tested to the blank control group and the negative control group. Continue to culture in the incubator for 48, then discard the culture medium and wash twice with 1mL PBS. Add 100μL of 10% DMSO and 1mol / L NaOH solution (accurately weigh 2g NaOH and dissolve it in 50mL deionized water, take 36mL and add 4mL DMSO), place in an 80℃ water bath for 45min to completely dissolve the cell clumps. After appropriate shaking, transfer the cell extracts of each group in the 6-well plate to a 96-well plate, and measure the OD at a wavelength of 405nm with an enzyme reader. The results are as follows Figure 2 shown.

[0146] The formula for calculating melanin content is as follows:

[0147] Melanin content = (OD1-OD3) / (OD2-OD3)×100%

[0148] Where:

[0149] OD1: absorbance value of the sample group;

[0150] OD2: absorbance value of negative control group;

[0151] OD3: absorbance value of the blank group.

[0152] By Figure 2 It can be seen that compared with the blank group, the sample with a concentration of 1.25 mg / mL is lower than the control, which may be because at this concentration, the sample has a certain degree of damage to the cells, so the melanin content is low; at a sample concentration of 156.25 μg / mL, the various plant extract compositions provided in Example 3 of the present invention can increase the melanin content in B16 melanoma cells to 113.20%, which is statistically significant (P<0.0001). The results suggest the potential role of the composition of Example 3 in promoting melanin production. Melanin production is directly related to hair color, so this finding indicates that the composition of this ratio helps to increase hair pigmentation, make hair darker or improve the uniformity of hair color. This effect is beneficial for improving gray hair, slowing down the graying process of hair, and enhancing the overall appearance of hair.

[0153] Example 3 Determination of the effect of the composition on the tyrosinase production rate of B16 mouse melanoma cells.

[0154] Tyrosinase activity in B16 mouse melanoma cells was measured by L-DOPA oxidation. The density of B16 cells in good growth condition was adjusted to 10 5 / mL, added to 96-well plate at 100μL / well, and cultured in a constant temperature incubator at 37℃ and 5% CO2 for 24h. B16 mouse melanoma cells were divided into negative control group, sample group and blank group, with 3 parallel wells in each group, and the experiment was repeated 3 times. After the cells adhered to the wall, 100μL of normal culture medium was added to the negative control group, and 100μL of culture medium containing samples of different concentrations of the composition of Example 1 was added to the sample group. After incubation for 48h, the supernatant was discarded, PBS was rinsed once, and 50μL of 1% Triton-X100 aqueous solution was added to each well. Quickly put it in a -80℃ refrigerator for 30min, take it out and thaw it at 37℃ (about 20min) to completely rupture the cells. Then add 100μL / well of 0.1% L-DOPA solution (0.1% L-DOPA solution: 0.01g of L-DOPA powder dissolved in 10mL PBS), and react at 37℃ for 2h. The OD value was measured at 475 nm using an enzyme marker. Figure 3 The formula for calculating tyrosinase activity in B16 melanoma cells is as follows:

[0155] Tyrosinase activity (%) = (OD1-OD3) / (OD2-OD3) × 100%

[0156] Where:

[0157] OD1: absorbance value of the sample group;

[0158] OD2: absorbance value of negative control group;

[0159] OD3: absorbance value of the blank group.

[0160] Depend on Figure 3 The results show that compared with the blank group, the tyrosinase activation rates at different concentrations were 106.40%, 118.53%, 109.43%, 104.22%, and 96.67%, respectively. Except for the latter two concentrations, the other concentrations were statistically different (P<0.0001). Compared with the above-mentioned melanin experiment, it was found that the trend of change in melanin content was consistent with the trend of change in tyrosinase activity. At a concentration of 156.25 μg / mL, the tyrosinase activity was the highest. At 1.25 mg / mL, due to certain cytotoxicity, the tyrosinase activity was the lowest. Therefore, the black hair efficacy mechanism of the composition of the present invention is to promote tyrosinase activity, increase the content of melanin production, promote melanin production in hair follicles, and pigmentate the hair color to make it appear black.

[0161] Part III Application Formula Safety and Efficacy Testing

[0162] Example 3: Human hair darkening test of the composition.

[0163] 20 volunteers aged 35-50 with gray hair were selected to apply the composition of Example 3 above, 3 mL per night, rubbing for 3 minutes, and observed and scored after continuous use for 4 months, and the average score was calculated. The evaluation criteria for observation and scoring are as follows:

[0164] The highest score is 5 points and the lowest score is 1 point.

[0165] 5 points: All new hair turns black;

[0166] 4 points: The roots of the new hair turn black and the part above the roots is black;

[0167] 3 points: The roots of the new hair are black, and the part above the roots is light black;

[0168] 2 points: The roots of the new hair are light black, and the part above the roots is basically white;

[0169] 1 point: The root of the new hair and the parts above it do not show black; the above score can be a non-integer score according to the actual situation. The results are shown in Table 3.

[0170] Table 3 Volunteers' ratings of usage

[0171] volunteer Fraction volunteer Fraction 1 3.7 11 3.4 2 3.6 12 3.8 3 4.0 13 3.5 4 3.8 14 4.0 5 4.1 15 3.9 6 3.5 16 4.1 7 3.6 17 4.3 8 3.8 18 3.1 9 3.5 19 3.8 10 3.1 20 3.6

[0172] From Table 3 and Figure 4-Figure 7 As a result, the composition of Example 3 had an average score of 3.71. After continuous use, the composition of the present invention showed a good hair darkening effect, and the roots of white hair and the hair above the roots became black in 4 months.

[0173] The above results show that the blackening and hair growth composition of the present invention has multiple effects on hair follicles and scalp, which can not only promote hair growth and nourish hair, but also promote the production of melanin in hair follicles, darken the hair color, and make the hair look healthier and more lustrous. The various natural plant compositions of the present invention are mild and non-irritating, refreshing on the skin, and highly safe for long-term use. In addition, the present invention adopts nanotechnology, and the product has more active ingredient content, better delivery effect, and is more conducive to the absorption of hair follicles, which maximizes the effect of promoting blackening and blackening hair. In addition, the preparation method of the cosmetics for blackening and hair growth provided by the present invention is simple and convenient, which is conducive to large-scale production and application.

[0174] The above description is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several modifications or equivalent substitutions can be made to the technical solution without departing from the principle of the present invention. These modifications or equivalent substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A Chinese medicine composition for blackening and growing hair, characterized in that: The invention comprises the following raw materials in parts by weight: 3-6 parts of Polygonum multiflorum, 3-6 parts of Angelica sinensis, 2-5 parts of Oroxylum indica and 3-5 parts of Black Wolfberry.

2. The Chinese medicine composition according to claim 1, characterized in that: It also includes one or more of the following raw materials by weight: 2-4 parts of Ligustrum lucidum fruit, 3-5 parts of ginseng, 3-5 parts of Platycladus orientalis leaves and 3-4 parts of mint.

3. The Chinese medicine composition according to claim 2, characterized in that: The invention comprises the following raw materials in parts by weight: 3-6 parts of Polygonum multiflorum, 3-6 parts of Angelica sinensis, 2-5 parts of Oroxylum indica, 3-5 parts of Lycium barbarum, 2-4 parts of Ligustrum lucidum, 3-5 parts of Ginseng, 3-5 parts of Platycladus orientalis leaves and 3-4 parts of Mint.

4. The Chinese medicine composition according to claim 3, characterized in that: The invention comprises the following raw materials in parts by weight: 5 parts of Polygonum multiflorum, 5 parts of Angelica sinensis, 4 parts of Oroxylum indica, 5 parts of Lycium barbarum, 3 parts of Ligustrum lucidum, 4 parts of Ginseng, 5 parts of Platycladus orientalis leaves and 3 parts of Mint.

5. A method for preparing the Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: (1) mixing the raw materials with water, heating them, laying them down and letting them stand to obtain a sample solution; (2) heating the sample solution obtained in step (1) with microwaves to obtain a microwave pretreated sample; (3) stirring and dissolving the microwave pretreated sample obtained in step (2) in ultrapure water to prepare a nano spray drying preparatory solution; (4) Atomizing and drying the nano spray drying preliminary solution obtained in step (3) to obtain nanoparticles, that is, obtaining the traditional Chinese medicine composition.

6. Use of the traditional Chinese medicine composition according to any one of claims 1 to 4 or the traditional Chinese medicine composition prepared by the preparation method according to claim 5 in the preparation of cosmetics.

7. A cosmetic for blackening and growing hair, characterized in that: The invention comprises the Chinese medicine composition according to any one of claims 1 to 4 or the Chinese medicine composition prepared by the preparation method according to claim 5 and an auxiliary material acceptable in cosmetics.

8. The cosmetic according to claim 7, characterized in that: The mass percentage of the traditional Chinese medicine composition in the cosmetics is 0.01-10%.

9. The cosmetic according to claim 7, characterized in that: The auxiliary materials include one or more of emulsifiers, thickeners, humectants, oils, chelating agents and preservatives.

10. The cosmetic according to claim 9, characterized in that: The emulsifier comprises one or more of organic olive oil emulsifying wax, self-emulsifying monoglyceride, methyl glucoside sesquistearate and glyceryl monostearate; and / or The thickener comprises one or more of carbomer, polyacrylate cross-linked polymer and hydroxyethyl cellulose; and / or The moisturizing agent comprises one or more of glycerin, propylene glycol and sodium hyaluronate; and / or The oil comprises one or more of caprylic triglyceride, capric triglyceride, shea butter, isohexadecane, isopropyl palmitate, dimethicone and cyclopentasiloxane; and / or The chelating agent comprises disodium edetate; and / or The preservatives include one or more of phenoxyethanol, methylparaben, propylparaben and sodium benzoate.

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