Temperature-sensitive sensor of gold nanorod doped liquid photonic crystal

Abstract

The invention belongs to the technical field of material science, and relates to a gold nanorod doped liquid photonic crystal temperature-sensitive sensor, which is prepared from the following components in percentage by volume: 20 to 40 percent of SiO2, 32 to 50 percent of auxiliary solvent, 20 to 38 percent of glycerol and 0.01 to 0.8 percent of gold nanorod based on the mass of a mixed solution of glycerol, silicon dioxide and auxiliary solvent, wherein the length-diameter ratio of the gold nanorod is 1: 4; the particle size of the SiO2 microspheres is 130 nm to 200 nm; by doping the gold nanorod with proper size and excellent photo-thermal performance in the multi-component mixed solvent and changing the doping mass of the gold nanorod, temperature devices in different thermal therapy temperature application stages can be prepared, the sensitivity is high, and the reflectivity can reach several times at room temperature; the method is simple in operation, easy to detect, and good in structural color mutation, stability and responsiveness when reaching a thermal therapy temperature range, and can quickly respond within 5 minutes.

Description

Technical Field

[0001] The invention belongs to the technical field of polymer materials, and in particular relates to a temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal. Background Art

[0002] In recent years, due to the challenges faced by tumor treatment, people are actively exploring a treatment method that is both highly effective and has fewer side effects. As an emerging clinical application technology, local hyperthermia treatment of tumors has reached a fairly mature level in both research and practical application. At present, a variety of methods for treating tumor hyperthermia include radio frequency, microwave, ultrasound, alternating magnetic field and other heating technologies. These technologies have been applied in clinical practice and are being further studied and explored. However, how to accurately determine the specific location of the tumor, and based on this, accurately measure the temperature of the superficial or deep tumor heating area, and accurately grasp the required heating dose by controlling the temperature increase range of the tumor heating area, is still the core problem faced by these thermal therapy technologies.

[0003] There are two main tasks in using local hyperthermia treatment technology for cancer to achieve biological barrier temperature detection and management: one is to correctly raise the temperature of cancer cells to a reasonable therapeutic temperature range and maintain this temperature for a period of time, thereby causing destructive damage to cancer cells and lesions; from another perspective, people must also ensure that human tissues are protected from the effects of overheating and try their best to reduce various injuries that patients cannot accept during the medical process and possible life-threatening complications.

[0004] In the field of deep body temperature detection in human tissues, the most commonly used body temperature verification technologies can be roughly divided into two categories: the first is invasive temperature measurement technology, also called lossy temperature measurement, and the second is non-destructive temperature measurement, so it is also called non-invasive temperature measurement. Invasive temperature measurement technology usually refers to the use of metal measurement probes, or the use of metal needles and other tools to insert it directly into the body. Commonly used probe types are mainly thermal resistance probes or medical thermocouples. However, there may be many difficulties when measuring temperature through these probes. For example, due to the influence of high-frequency electromagnetic waves, it may not calculate the temperature very accurately, and it may also cause difficulty in reading; from another perspective, the sensor wire may affect the layout of the magnetic field. The implantation of the probe may cause pain to the patient, and it is not recommended to stay in the tissue for a long time, which may have an adverse effect on the treatment effect of the tumor.

[0005] Non-destructive temperature detection of biological tissues during hyperthermia has always been a hot topic of concern to researchers, and is also a key step in the early practical application and transformation of tumor hyperthermia into medicine. At present, the mainstream temperature measurement methods include nuclear magnetic resonance temperature measurement, microwave temperature measurement, X-ray diffraction temperature measurement, electrical impedance tomography temperature measurement, infrared thermal imager temperature measurement, etc.

[0006] Magnetic resonance can detect the temperature distribution of tissues through protons. The main existing calorimeter is the proton resonance frequency calorimeter, which works on the principle that the resonance frequency of water protons is related to temperature. If you want to derive a thermal relationship, you generally use the difference between the images, and after subtraction, you get a phase difference map, whose pixel matrix value is proportional to the temperature change. However, this temperature measurement method is difficult to develop and is expensive, which is not conducive to practical application.

[0007] Microwave temperature measurement technology is a simulation algorithm involving many complex aspects, such as the electrical reactance of tissues, radiation heating coefficient, etc. It uses the attenuation coefficient of radiation heating characteristics to change with temperature, combined with the heat transfer equation. However, ultra-high frequency electromagnetic waves will inevitably produce strong electromagnetic fields, which will bring difficulties to temperature measurement. The induced current generated by the temperature probe will bring great errors to the temperature measurement or make it difficult to measure the temperature stably. At the same time, due to the low accuracy and low resolution of microwave temperature measurement, it is still questionable in practical applications.

[0008] Infrared thermal imagers mainly determine the surface temperature of the object being measured based on the intensity of infrared radiation released by the object. It is a non-contact temperature detection, and due to this characteristic, it can not destroy the temperature of biological tissue. However, infrared temperature measurement is also affected by several key factors: the emissivity of the object, the aerosol and the distance of the light spot, and its application range is greatly limited. At the same time, because in the application of thermal therapy, the tumor is generally located deep in the tissue, the surface non-contact temperature measurement of the infrared thermometer is incapable of meeting the requirements.

[0009] Electrical impedance tomography temperature measurement technology is essentially based on modeling and calculation based on the electrical properties of different biological tissues under various temperature conditions. The algorithm has a high degree of complexity and low spatial resolution, which also leads to unsatisfactory temperature measurement accuracy and cannot meet the standards of clinical application. The X-ray diffraction method that has emerged in recent years is a temperature measurement method that determines the temperature field distribution by detecting the absorption characteristics of particles in the tissue temperature field. Although this temperature measurement method is simple and effective, it has a large accuracy error.

[0010] In the hyperthermia therapy of tumors, the determination of biological tissue temperature is an extremely difficult and challenging task, and it is also an important problem that needs to be solved urgently. Non-destructive temperature measurement technology is a research hotspot and a general trend in tumor hyperthermia measurement methods. Proton resonance calorimeter and electrical impedance temperature measurement both have a relatively complete theoretical basis in temperature measurement. However, in practical application conversion, there is still a lack of practice. Through further optimization and experimentation, these temperature measurement technologies are expected to become the mainstream means in clinical temperature measurement. In the field of microwave temperature measurement, only when we make significant optimizations in improving the accuracy and spatial resolution of temperature measurement, accelerating the speed of microwave CT imaging, and broadening the observation range, can we truly enter a new stage of practical application. Although the electrical impedance method has shown certain potential in temperature measurement, more in-depth theoretical research is still needed.

[0011] A novel temperature measurement method is proposed in the present invention, which can also be used as a verification method for conventional temperature measurement. That is, the drug loading concentration of gold nanorods is determined, and they are equivalently doped in the near-infrared responsive liquid photonic crystals of the present invention. Under the excitation of near-infrared lasers, the temperature range of the drug at the tumor site is equivalent to the temperature of the thermosensitive photonic crystal, and its temperature detection is relatively accurate, and the temperature can be characterized by the color mutation and reflectivity mutation of the photonic crystal. By changing the concentration or size of gold nanorods, thermal therapy at different temperature stages can be achieved, such as conventional thermal therapy: 40-45°C, thermal ablation: 60°C. The present invention innovatively dopes multi-component solvents and solutes without destroying the original properties of the photonic crystal, which is expected to bring a new research direction to the existing non-destructive temperature measurement technology. Summary of the invention

[0012] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a temperature-sensitive sensor of gold nanorods doped with liquid photonic crystals.

[0013] In order to achieve the purpose of the present invention, the present invention will be implemented by adopting the technical solution described below.

[0014] A temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal is composed of 20%-40% SiO2, 32%-50% auxiliary solvent and 20%-38% propylene glycol by volume, and 0.01‰-0.8‰ gold nanorods by mass of a mixed solution of propylene glycol, silicon dioxide and auxiliary solvent; wherein the aspect ratio of the gold nanorods is 1:4; and the particle size of the SiO2 microspheres is 130nm-200nm.

[0015] As a preferred embodiment of the present invention, the auxiliary solvent is an organic solvent with a high dielectric constant, including one or a mixture of two or a mixture of three of ethylene carbonate, propylene carbonate, and dimethyl carbonate.

[0016] As a preferred embodiment of the present invention, the optimized particle size of the SiO2 microspheres is 180 nm.

[0017] A method for preparing a temperature sensitive sensor comprises the following steps: S31, preparing a liquid photonic crystal mixed solution, comprising 20%-40% SiO2, 32%-50% auxiliary solvent, 20%-38% glycerol by volume, and 0.01‰-0.8‰ gold nanorods by mass of the mixed solution of glycerol, silicon dioxide and auxiliary solvent; wherein the aspect ratio of the gold nanorods is 1:4; and the particle size of the SiO2 microspheres is 130nm-200nm; S32, putting the mixed solution prepared in S31 into a 1 mL centrifuge tube and placing it in an oven, evaporating it to a supersaturated state, and keeping it warm until the mixed solution turns into a transparent colloidal state; wherein the evaporation temperature of the oven is 60°C-100°C, and the keeping time is 1h-12h; S33, sandwiching the transparent colloidal photonic crystal obtained in S32 between PMMA sheets and fixing them with UV curing adhesive to form a temperature sensitive sensor.

[0018] As a preferred embodiment of the present invention, the evaporation temperature is 90° C. and the insulation time is 2 hours.

[0019] As a preferred embodiment of the present invention, the thickness of the PMMA sheet is in the range of 50 μm to 300 μm.

[0020] A temperature-sensitive sensor is used in detecting the temperature of hyperthermia. The temperature-sensitive sensor is prepared by 20%-40% SiO2, 32%-50% auxiliary solvent and 20%-38% propylene glycol by volume, and 0.01‰-0.8‰ gold nanorods by mass of a mixed solution of propylene glycol, silica and auxiliary solvent. The temperature-sensitive sensor is irradiated by a near-infrared laser with a power of 0.5W-10W and a wavelength of 808 nm for 30s-10min. The structural color of the liquid photonic crystal tends to saturation and the brightness increases. The aspect ratio of the gold nanorod is 1:4. The particle size of the SiO2 microspheres is 130nm-200nm. The mutation temperature of the structural color of the liquid photonic crystal is between 25-65 degrees.

[0021] As a preferred embodiment of the present invention, when the temperature sensitive sensor detects the temperature of tumor hyperthermia, the near-infrared irradiation laser is irradiated with a near-infrared laser having a power of 2W and a wavelength of 808 nm, and the irradiation time is determined according to the inactivation state of the cancer cells.

[0022] Beneficial effects: The present invention uses bionic photonic crystals as the substrate, and utilizes supersaturated evaporation and a series of chemical reactions to achieve the preparation of temperature-sensitive photonic crystals, creating a fast, simple and effective method to monitor the temperature range in the field of tumor photothermal therapy, or to verify the temperature range. The prepared material completely retains the characteristics of solid-state photonic crystals: photon bandgap phenomenon, good photon transmission performance and periodic dielectric constant, which greatly improves the temperature-light response performance. By controlling the content of glycerol and gold nanorods, liquid photonic crystals with different temperature responses can be selectively prepared.

[0023] Traditional thermotherapy temperature detection technology generally uses a probe method to insert a sensor under the patient's skin tissue, and the accuracy of the temperature is also a potential problem. Compared with traditional technology, the present invention does not require additional equipment to analyze the data, and can directly obtain a more accurate temperature range by naked eye observation. At the same time, in vitro non-invasive monitoring and simple process flow also provide strong support for this monitoring method. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The schematic diagram of the liquid photonic crystal using multi-component glycerol-containing near-infrared laser temperature response; Figure 2 Figure (a) is a spectrum diagram, digital photo and thermal image diagram corresponding to 35°C in the tumor hyperthermia treatment of Example 3; Figure (b) is a spectrum diagram, digital photo and thermal image diagram corresponding to 40°C in the tumor hyperthermia treatment of Example 6; Figure (c) is a spectrum diagram, digital photo and thermal image diagram corresponding to 45°C in the tumor hyperthermia treatment of Example 9; Figure (d) is a spectrum diagram, digital photo and thermal image diagram corresponding to 60°C in the tumor hyperthermia treatment of Example 12; Figure 3 Thermal imaging and digital photos of the liquid photonic crystal for monitoring the temperature of thermal therapy prepared by changing the SiO2 particle size to 155 nm in Example 5. DETAILED DESCRIPTION

[0025] The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. The following embodiments are implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operation processes are given, but the protection scope of the present invention is not limited to the following embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] As embodiment 1 of the present invention, Figure 1 and Figure 2As shown in the figure, a temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal contains 30% SiO2 microspheres with a particle size of 188 nm, 26% propylene glycol, 44% propylene carbonate, 0.05‰ gold nanorods and an aspect ratio of 1: 4. According to the gradient experiment, when the system components contain 26% propylene glycol, the mutation temperature of the liquid photonic crystal structural color is between 32-35 degrees.

[0027] As embodiment 2 of the present invention, Figure 1 As shown, a method for preparing a temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal comprises the following steps: Step 1: Centrifuge 50 μL of 200 mg / mL gold nanorod solution at 10,000 r / min for 10 min into a 2 mL glass centrifuge tube, discard the supernatant, add 50 μL of silica microspheres, and then add 1 mL of anhydrous ethanol solution, shake well and let stand for 2 min for use.

[0028] Step 2: Add 43 μL of glycerol and 73 μL of propylene carbonate to the centrifuge tube and centrifuge and shake until mixed evenly.

[0029] Step 3: Place the prepared mixed solution in a 90°C oven for 2 hours, take it out every 30 minutes and shake it to mix it evenly. After cooling to room temperature, a near-infrared responsive thermosensitive photonic crystal is obtained.

[0030] Step 4: Take 20 μL of the temperature-sensitive photonic crystal and place it between two clean PMMA acrylic plates using a 200 μm template for UV glue curing to obtain the temperature-sensitive sensor in Example 1.

[0031] As embodiment 3 of the present invention, Figure 1 and Figure 2 As shown in Figure (a), a temperature-sensitive sensor is used to detect the temperature of hyperthermia. After the temperature-sensitive sensor prepared by the preparation method described in Example 2 is irradiated with a near-infrared laser with a power of 2 W and a wavelength of 808 nm for 5 minutes, the temperature rises to 35.3°C and the reflectivity increases to 57.3%. At the same time, a sudden change and enhancement of the structural color occur.

[0032] As embodiment 4 of the present invention, Figure 1 and Figure 2 As shown in the figure, a temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal has SiO2 microspheres with a particle size of 188 nm, 30% SiO2 microspheres, 34% glycerol, 36% propylene carbonate, 0.1‰ gold nanorod mass and an aspect ratio of 1:4. According to the gradient experiment, when the system components contain 34% glycerol, the mutation temperature of the liquid photonic crystal structural color is between 38-40 degrees, which corresponds to the 40-degree treatment range in hyperthermia.

[0033] As embodiment 5 of the present invention, Figure 1 As shown, a method for preparing a temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal comprises the following steps: Step 1: Centrifuge 100 μL, 200 mg / mL gold nanorod solution at 10,000 r / min for 10 min into a 2 mL glass centrifuge tube, discard the supernatant, add 50 μL of silica microspheres, and then add 1 mL of anhydrous ethanol solution, shake well and let stand for 2 min for use; Step 2: Add 56 μL of glycerol and 60 μL of propylene carbonate to the centrifuge tube, and centrifuge and shake until the mixture is evenly mixed; Step 3: Place the prepared mixed solution in a 90°C oven for 2 hours, take it out every 30 minutes for shaking and mixing, and wait for it to cool to room temperature to obtain a near-infrared responsive thermosensitive photonic crystal; Step 4: Take 20 μL of the temperature-sensitive photonic crystal and place it between two clean PMMA acrylic plates using a 200 μm template for UV glue curing to obtain the temperature-sensitive sensor described in Example 4.

[0034] As embodiment 6 of the present invention, Figure 1 and Figure 2 As shown in Figure (b), a temperature-sensitive sensor is used to detect the temperature of hyperthermia. After the temperature-sensitive sensor prepared by the preparation method described in Example 5 is irradiated with a near-infrared laser with a power of 2 W and a wavelength of 808 nm for 5 minutes, the temperature rises to 40.4°C and the reflectivity increases to 68.3%. At the same time, a sudden change and enhancement of the structural color occur.

[0035] As embodiment 7 of the present invention, Figure 1 and Figure 2 As shown, a temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal has SiO2 microspheres with a particle size of 188 nm, 30% SiO2 microspheres, 36% glycerol, 34% propylene carbonate, 0.15‰ gold nanorod mass and an aspect ratio of 1: 4. According to the gradient experiment, when the system components contain 36% Gly, the structural color mutation of the liquid photonic crystal is approximately between 42-44 degrees, which corresponds to the 45-degree treatment range in hyperthermia.

[0036] As embodiment 8 of the present invention, Figure 1 As shown, a method for preparing a temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal comprises the following steps: Step 1: Centrifuge 150 μL, 200 mg / mL gold nanorod solution at 10,000 r / min for 10 min into a 2 mL glass centrifuge tube, discard the supernatant, add 50 μL of silica microspheres, and then add 1 mL of anhydrous ethanol solution, shake well and let stand for 2 min for use.

[0037] Step 2: Add 60 μL of glycerol and 56 μL of propylene carbonate to the centrifuge tube and centrifuge and shake until mixed evenly.

[0038] Step 3: Place the prepared mixed solution in a 90°C oven for 2 hours, take it out every 30 minutes and shake it to mix it evenly. After cooling to room temperature, a near-infrared responsive thermosensitive photonic crystal is obtained.

[0039] Step 4: Take 20 μL of the temperature-sensitive photonic crystal and place it between two clean PMMA acrylic plates using a 200 μm template for UV glue curing to obtain the temperature-sensitive sensor described in Example 7.

[0040] As embodiment 9 of the present invention, Figure 1 and Figure 2 As shown in Figure (c), a temperature-sensitive sensor is used to detect the temperature of thermal therapy. After the temperature-sensitive sensor prepared by the preparation method described in Example 8 is irradiated with a near-infrared laser with a power of 2 W and a wavelength of 808 nm for 5 minutes, the temperature is increased to 45.4°C and the reflectivity is increased to 74.9%. At the same time, a mutation and enhancement of the structural color occur.

[0041] As embodiment 10 of the present invention, Figure 1 and Figure 2 As shown, a temperature-sensitive sensor of gold nanorods doped with liquid photonic crystals contains 30% SiO2 microspheres with a particle size of 188 nm, 24% propylene glycol, 46% propylene carbonate, a mass fraction of gold nanorods of 0.5‰ and an aspect ratio of 1:4.

[0042] As Example 11 of the present invention, Figure 1 As shown, a method for preparing a temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal comprises the following steps: Step 1: Centrifuge 500 μL of 200 mg / mL gold nanorod solution at 10,000 r / min for 10 min into a 2 mL glass centrifuge tube, discard the supernatant, add 50 μL of silica microspheres, and then add 1 mL of anhydrous ethanol solution, shake well and let stand for 2 min for use.

[0043] Step 2: Add 40 μL of glycerol and 76 μL of propylene carbonate to the centrifuge tube and centrifuge and shake until mixed evenly.

[0044] Step 3: Place the prepared mixed solution in a 90°C oven for 2 hours, take it out every 30 minutes and shake it to mix it evenly. After cooling to room temperature, a near-infrared responsive thermosensitive photonic crystal is obtained.

[0045] Step 4: Take 20 μL of the temperature-sensitive photonic crystal and place it between two clean PMMA acrylic plates using a 200 μm template for UV glue curing to obtain the temperature-sensitive sensor described in Example 10.

[0046] As Example 12 of the present invention, Figure 1 and Figure 2 As shown in Figure (d), a temperature-sensitive sensor is used to detect the temperature of hyperthermia. After the temperature-sensitive sensor prepared by the preparation method described in Example 11 is irradiated with a near-infrared laser with a power of 2 W and a wavelength of 808 nm for 5 minutes, the temperature rises to 60.6°C and the reflectivity increases to 78.2%. At the same time, a sudden change and enhancement of the structural color occurs.

[0047] Depend on Figure 2 It can be seen that increasing the concentration of the gold nanorod solution with an aspect ratio of 1:4 can improve its photothermal conversion performance. However, if it is added in excess, it will affect the structural color formation of the liquid photonic crystal. Generally speaking, the mass of the gold nanorods does not exceed 1‰ of the system. When the near-infrared irradiation and the assembly between the SiO2 microspheres reach equilibrium, continued irradiation generally has no effect on the system. However, when the system temperature exceeds 70°C, due to the overheated evaporation and edge diffusion effects and the uneven thickness of the photonic crystal layer, a slight wavelength blue shift will occur. However, considering that in actual applications, the maximum temperature of thermal therapy is about 60°C, this situation is not considered.

[0048] As Example 13 of the present invention, Figure 1 and Figure 2 As shown, a temperature-sensitive sensor of gold nanorods doped with liquid photonic crystals contains 30% SiO2 microspheres with a particle size of 150 nm, 36% glycerol, 34% propylene carbonate, a mass fraction of gold nanorods of 0.15‰ and an aspect ratio of 1:4.

[0049] As Embodiment 14 of the present invention, Figure 1 As shown, a method for preparing a temperature-sensitive sensor of a gold nanorod-doped liquid photonic crystal comprises the following steps: Step 1: Centrifuge 150 μL of 200 mg / mL gold nanorod solution at 10,000 r / min for 10 min into a 2 mL glass centrifuge tube, discard the supernatant, add 50 μL of silica microspheres, and then add 1 mL of anhydrous ethanol solution, shake well and let stand for 2 min for use.

[0050] Step 2: Add 60 μL of glycerol and 56 μL of propylene carbonate to the centrifuge tube and centrifuge and shake until the mixture is evenly mixed.

[0051] Step 3: Place the prepared mixed solution in a 90°C oven for 2 hours, take it out every 30 minutes and shake it to mix it evenly. After cooling to room temperature, a near-infrared responsive thermosensitive photonic crystal is obtained.

[0052] Step 4: Take 20 μL of the temperature-sensitive photonic crystal and place it between two clean PMMA acrylic plates using a 200 μm template for UV glue curing to obtain the temperature-sensitive sensor described in Example 13.

[0053] As Example 15 of the present invention, Figure 1 and Figure 3 As shown, a temperature-sensitive sensor is used to detect the temperature of hyperthermia. After the temperature-sensitive sensor prepared by the preparation method described in Example 14 is irradiated with a near-infrared laser with a power of 2 W and a wavelength of 808 nm for 5 minutes, the temperature rises to 44.8°C, and a sudden change and enhancement of the structural color occurs.

[0054] The preferred embodiments of the present invention are described above with reference to the accompanying drawings, but the scope of the rights of the present invention is not limited thereto. Any modification, equivalent substitution and improvement made by a person skilled in the art without departing from the scope and essence of the present invention should be within the scope of the rights of the present invention.

Claims

1. A temperature-sensitive sensor of gold nanorods doped with liquid photonic crystals, characterized in that: The temperature sensitive sensor is composed of 20%-40% SiO2, 32%-50% auxiliary solvent and 20%-38% propylene glycol by volume, and 0.01‰-0.8‰ gold nanorods by mass of a mixed solution of propylene glycol, silica and auxiliary solvent; wherein the aspect ratio of the gold nanorods is 1:4; and the particle size of the SiO2 microspheres is 130nm-200nm.

2. The temperature sensitive sensor of the gold nanorod-doped liquid photonic crystal according to claim 1, characterized in that: The auxiliary solvent is an organic solvent with a high dielectric constant, including one of ethylene carbonate, propylene carbonate, and dimethyl carbonate, or a mixture of two of them or a mixture of three of them.

3. The temperature sensitive sensor of the gold nanorod-doped liquid photonic crystal according to claim 1, characterized in that: The particle size of the SiO2 microspheres is 180 nm.

4. A method for preparing a temperature sensitive sensor, characterized in that: The following steps are involved: S31, preparing a liquid photonic crystal mixed solution, comprising 20%-40% SiO2, 32%-50% auxiliary solvent, and 20%-38% glycerol by volume, and 0.01‰-0.8‰ gold nanorods by mass of the mixed solution of glycerol, silicon dioxide, and auxiliary solvent; S32, putting the mixed solution prepared in S31 into a 1 mL centrifuge tube and placing it in an oven, evaporating it to a supersaturated state, and keeping it warm until the mixed solution turns into a transparent colloidal state; wherein the evaporation temperature of the oven is 60° C.-100° C., and the keeping time is 1 h-12 h; S33, sandwiching the transparent colloidal photonic crystal obtained in S32 between PMMA sheets and fixing them with UV curing adhesive to form a temperature sensitive sensor.

5. The method for preparing a temperature sensitive sensor according to claim 4, characterized in that: The thickness of the PMMA sheet ranges from 50 μm to 300 μm.

6. An application of a temperature sensitive sensor in detecting hyperthermia temperature, characterized in that: A temperature-sensitive sensor made of 20%-40% SiO2, 32%-50% auxiliary solvent and 20%-38% glycerol by volume and 0.01‰-0.8‰ gold nanorods by mass of a mixed solution of glycerol, silica and auxiliary solvent is irradiated under a near-infrared laser with a power of 0.5W-10W and a wavelength of 808nm for 30s-10min. It can be observed that the liquid photonic crystal structural color tends to saturation and the brightness increases. The aspect ratio of the gold nanorods is 1:

4. The particle size of the SiO2 microspheres is 130nm-200nm. The mutation temperature of the liquid photonic crystal structural color is between 25-65℃.

7. The use according to claim 6, characterized in that When the temperature-sensitive sensor detects the temperature of tumor hyperthermia, the near-infrared laser is irradiated with a near-infrared laser having a power of 2W and a wavelength of 808 nm, and the irradiation time is 5-20 minutes, which is determined by the inactivation state of the cancer cells.

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