Traditional Chinese medicine film spraying agent for promoting healing of diabetic refractory wounds and preparation method of traditional Chinese medicine film spraying agent

Abstract

The invention discloses a traditional Chinese medicine spray film agent for promoting healing of diabetic refractory wounds and a preparation method, the traditional Chinese medicine spray film agent adopts traditional Chinese medicine extracts such as rheum officinale, rhizoma bletillae, pseudo-ginseng, liquorice and the like, through scientific proportioning, multiple synergistic effects of resisting inflammation, stopping bleeding, resisting oxidation and promoting blood circulation are achieved, and through combination of rhizoma bletillae polysaccharide and PVP-K30, the slow-release effect of the medicine is optimized, so that the effect of promoting healing of the diabetic refractory wounds is achieved. A protective film is formed to continuously release the pesticide effect. The film spraying agent can obviously improve the microcirculation condition of the wound of a diabetic patient, promote the formation of new tissues and reduce the infection risk. Compared with the prior art, the invention obviously accelerates the healing process of refractory wounds of diabetes mellitus, reduces side effects, and has good stability and safety.

Description

Technical Field

[0001] The invention relates to the technical field of diabetic wound healing treatment, in particular to a traditional Chinese medicine spray film for promoting the healing of diabetic refractory wounds and a preparation method thereof. Background Art

[0002] Due to long-term poor blood sugar control, diabetic patients often suffer from microcirculatory disorders and weakened immune function, which makes it difficult for skin wounds to heal, especially for difficult-to-heal wounds such as lower limb ulcers and diabetic foot. These wounds are prone to infection during the healing process and the healing speed is extremely slow, which seriously affects the quality of life of patients.

[0003] In the prior art, the treatment methods for diabetic refractory wounds mainly include: using antibiotics to prevent and treat infection, applying topical ointments or dressings to promote wound healing, and performing surgical wound debridement and vascular reconstruction. However, these methods have the following major problems:

[0004] 1. Antibiotic abuse and drug resistance: Although long-term use of antibiotics can control infection to a certain extent, it can easily lead to drug resistance, resulting in a gradual decline in treatment effectiveness and even the risk of secondary infection.

[0005] 2. Limitations of topical drugs and dressings: Although the topical drugs and dressings currently in widespread use can protect the wound surface and promote healing to a certain extent, most of them can only provide short-term drug release and local protection, and it is difficult to exert the drug effect continuously. At the same time, the transdermal absorption effect of these drugs and dressings is limited, and they cannot effectively penetrate into deep tissues, making it difficult to improve microcirculation and promote the formation of new tissues.

[0006] 3. High risk of surgical intervention: For severe wounds such as diabetic foot, surgical intervention is a common treatment method, but the operation itself has a high risk, and auxiliary treatments such as drugs and dressings are still required after surgery, which can easily lead to wound recurrence or postoperative infection.

[0007] Therefore, how to effectively accelerate the healing of refractory diabetic wounds and reduce the risk of infection during the treatment process has become a technical problem to be solved by the present invention. Summary of the invention

[0008] The technical problem solved by the present invention is to provide a Chinese medicine spray film for promoting the healing of refractory diabetic wounds and a preparation method thereof in view of the defects existing in the above-mentioned prior art, so as to solve the problems of limited efficacy, high drug resistance, and inability to maintain effective effects in the treatment of refractory diabetic wounds raised in the above-mentioned background technology.

[0009] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows:

[0010] A Chinese medicine spray film for promoting the healing of refractory diabetic wounds, characterized in that the Chinese medicine spray film comprises the following components in percentage by weight:

[0011] Rhubarb extract: 1-10%;

[0012] Bletilla striata extract: 0.5-5%;

[0013] Panax notoginseng extract: 0.5-5%;

[0014] Licorice extract: 0.5-5%;

[0015] Bletilla striata polysaccharide: 2-10%;

[0016] Polyvinylpyrrolidone: 2-10%;

[0017] Tween-80: 0.1-5%;

[0018] Water: 60-90%.

[0019] As a further solution of the present invention, the pH value of the traditional Chinese medicine spray film is between 4.0 and 7.5.

[0020] As a further embodiment of the present invention, the extracts of rhubarb, bletilla striata, notoginseng and liquorice are obtained by water extraction and alcohol precipitation.

[0021] As a further solution of the present invention, the method for using the spray film comprises: spraying the spray film uniformly on the surface of the diabetic refractory wound, applying 2 to 3 times a day until the wound is healed.

[0022] As a further solution of the present invention, the method comprises applying the traditional Chinese medicine spray film according to any one of claims 1 to 4 to the surface of a refractory wound of a diabetic patient, applying it 2 to 3 times a day, and continuing to use it until the wound heals.

[0023] As a further embodiment of the present invention, the method includes thoroughly cleaning and disinfecting the wound before treatment, ensuring that the spray film evenly covers the wound surface, and avoiding wound contamination during treatment.

[0024] A method for preparing a Chinese medicine spray film for promoting the healing of refractory diabetic wounds, the preparation method comprising the following steps:

[0025] Step 1, mixing raw materials of rhubarb, bletilla striata, notoginseng and liquorice in a weight ratio of 2:1:1:1, and soaking in water for 30 minutes;

[0026] Step 2, heating the soaked mixture to boiling, turning to low heat and continuing to boil for 30 minutes, filtering to obtain the first decoction, repeating the process for the second decoction, and boiling the second decoction for 20 minutes;

[0027] Step 3, combining the first decoction and the second decoction, and continuing to filter to obtain a clear liquid;

[0028] Step 4, adding Bletilla striata polysaccharide and polyvinyl pyrrolidone to part of the clear solution at 50°C, and stirring until completely dissolved;

[0029] Step 5, adding the crude powder of the combined drug to the solution obtained in step 4, continuing to stir evenly, cooling to room temperature, adding Tween-80, continuing to stir evenly, and diluting to the target volume with clear liquid to obtain a traditional Chinese medicine spray film.

[0030] As a further solution of the present invention, in step 4, a magnetic stirrer is used during stirring, heating at a temperature of 50-60° C., and stirring is continued for 15-30 minutes until complete dissolution.

[0031] As a further embodiment of the present invention, the total decoction time of the clear liquid is 50 minutes, and the volume of the clear liquid after filtration is 2.5-3 times the weight of the initial raw material.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] 1. Multiple mechanisms synergistically act to significantly accelerate wound healing: The Chinese medicine spray film of the present invention uses a scientific combination of Chinese medicine extracts such as rhubarb, white schizonepeta, Panax notoginseng and licorice to give full play to the synergistic effects of these ingredients in anti-inflammatory, hemostatic, anti-oxidation, and blood circulation promotion. Among them, rhubarb has a strong anti-inflammatory effect and can effectively reduce the inflammatory response at the wound site; white schizonepeta and Panax notoginseng mainly accelerate the healing of the wound surface by promoting blood circulation and hemostasis; and licorice plays an antioxidant and immunomodulatory role. Through the combined effects of these mechanisms, the Chinese medicine spray film of the present invention can effectively regulate the various stages of wound healing, thereby significantly accelerating the healing speed of refractory wounds in diabetic patients.

[0034] 2. Optimized drug release mechanism to improve therapeutic effect: The Bletilla striata polysaccharide and PVP-K30 components in the present invention not only improve the adhesion of the spray film, but also optimize the sustained release effect of the drug. These components form a protective film on the surface of the wound, which not only prevents external pollutants from entering the wound, but also can continuously release the active ingredients of traditional Chinese medicine to maintain the effective concentration of the drug in the local wound. This sustained release effect is particularly suitable for the wound care needs of diabetic patients, and can provide continuous therapeutic effects for a long time, significantly improving the overall efficacy.

[0035] 3. Improve microcirculation and promote the formation of new tissue: Diabetic patients often have microcirculatory disorders due to poor blood sugar control, which makes it difficult for wounds to heal. The Panax notoginseng extract in the present invention has a significant effect of promoting blood circulation, can improve the local microcirculation of the wound, increase blood oxygen supply, and promote the formation of new tissue. At the same time, rhubarb and licorice extracts can also promote the proliferation of fibroblasts, accelerate the generation of collagen fibers, and ultimately promote the complete healing of wounds.

[0036] 4. Dual effects of antibacterial and anti-inflammatory, reducing the risk of infection: The components in the traditional Chinese medicine spray film of the present invention not only have anti-inflammatory effects, but also have certain antibacterial effects, effectively reducing the risk of wound infection in diabetic patients. Diabetic patients have low immunity and their wounds are prone to infection. The spray film of the present invention provides a more ideal healing environment for wounds through its dual effects of antibacterial and anti-inflammatory, thereby reducing the occurrence of complications and ensuring the smooth progress of treatment.

[0037] 5. Based on the natural ingredients of traditional Chinese medicine, side effects and drug resistance are reduced: the traditional Chinese medicine ingredients used in the present invention are all derived from natural plants and have good biocompatibility and safety. Compared with many traditional chemical drug treatment methods, the traditional Chinese medicine spray film of the present invention not only effectively avoids the side effects that may be caused by chemical drugs, but also reduces the drug resistance problem that may be caused by long-term use, which is particularly important for diabetic patients who need long-term treatment.

[0038] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0040] Figure 1 The figure is a schematic diagram of the skin tissue layer structure and the free inflammatory scab of the present invention.

[0041] Figure 2 This is a schematic diagram of the basic healing of the epithelium in the damaged area and the new spinous layer tissue of the present invention.

[0042] Figure 3 It is a schematic diagram of the new epithelial spinous layer and dermal scar tissue under the inflammatory scab of the present invention.

[0043] Figure 4 This is a schematic diagram of the disappearance of epithelium and coverage of inflammatory scabs in the injured area of ​​the present invention.

[0044] Figure 5 This is a schematic diagram of the basic healing of the epithelium and the scar tissue of the dermis in the present invention.

[0045] Figure 6 It is a schematic diagram of the epithelial healing trend of the injured area and the granulation tissue of the dermis in the present invention.

[0046] Figure 7 This is a schematic diagram of the disappearance of most of the epithelium in the damaged area and the proliferation of new epithelium at the edge of the injury area according to the present invention.

[0047] Figure 8 It is a schematic diagram of the basic healing of the epithelium and the infiltration of neutrophils under the inflammatory scab of the present invention.

[0048] Fig. 9 This is a schematic diagram of the replacement of granulation tissue in the dermis of a skin injury area according to the present invention.

[0049] Fig.10 This is a schematic diagram of the inflammatory scab and deep scar tissue in the skin injury area of ​​a normal wound of the present invention.

[0050] Fig.11 This is another schematic diagram of inflammatory scab and deep scar tissue in the skin injury area of ​​a normal wound according to the present invention. DETAILED DESCRIPTION

[0051] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0052] See also Figure 1 —11. In an embodiment of the present invention, a Chinese medicine spray film for promoting the healing of refractory diabetic wounds is characterized in that the Chinese medicine spray film comprises the following ingredients in percentage by weight:

[0053] Rhubarb extract: 1-10%;

[0054] Bletilla striata extract: 0.5-5%;

[0055] Panax notoginseng extract: 0.5-5%;

[0056] Licorice extract: 0.5-5%;

[0057] Bletilla striata polysaccharide: 2-10%;

[0058] Polyvinylpyrrolidone: 2-10%;

[0059] Tween-80: 0.1-5%;

[0060] Water: 60-90%.

[0061] As a further solution of the present invention, the pH value of the traditional Chinese medicine spray film is between 4.0 and 7.5.

[0062] As a further embodiment of the present invention, the extracts of rhubarb, bletilla striata, notoginseng and liquorice are obtained by water extraction and alcohol precipitation.

[0063] As a further solution of the present invention, the method for applying the spray film comprises: spraying the spray film uniformly on the surface of the diabetic refractory wound, applying 2 to 3 times a day until the wound is healed.

[0064] As a further solution of the present invention, the method comprises applying the traditional Chinese medicine spray film according to any one of claims 1 to 4 to the surface of a refractory wound of a diabetic patient, applying it 2 to 3 times a day, and continuing to use it until the wound heals.

[0065] As a further embodiment of the present invention, the method includes thoroughly cleaning and disinfecting the wound before treatment, ensuring that the spray film evenly covers the wound surface, and avoiding wound contamination during treatment.

[0066] A method for preparing the traditional Chinese medicine film spray according to claim 1, the method comprising the following steps:

[0067] Step 1, mixing raw materials of rhubarb, bletilla striata, notoginseng and liquorice in a weight ratio of 2:1:1:1, and soaking in water for 30 minutes;

[0068] Step 2, heating the soaked mixture to boiling, turning to low heat and continuing to boil for 30 minutes, filtering to obtain the first decoction, repeating the process for the second decoction, and boiling the second decoction for 20 minutes;

[0069] Step 3, combining the first decoction and the second decoction, and continuing to filter to obtain a clear liquid;

[0070] Step 4, adding Bletilla striata polysaccharide and polyvinyl pyrrolidone to part of the clear solution at 50°C, and stirring until completely dissolved;

[0071] Step 5, adding the crude powder of the combined drug to the solution obtained in step 4, continuing to stir evenly, cooling to room temperature, adding Tween-80, continuing to stir evenly, and diluting to the target volume with clear liquid to obtain a traditional Chinese medicine spray film.

[0072] As a further solution of the present invention, in step 4, a magnetic stirrer is used during stirring, heating at a temperature of 50-60° C., and stirring is continued for 15-30 minutes until complete dissolution.

[0073] As a further embodiment of the present invention, the total decoction time of the clear liquid is 50 minutes, and the volume of the clear liquid after filtration is 2.5-3 times the weight of the initial raw material.

[0074] Embodiment 1:

[0075] In the treatment of diabetic foot ulcers, patients suffer from lower limb microcirculation disorders due to long-term poor blood sugar control, which is often accompanied by decreased immunity and difficulty in wound healing. Existing treatment methods mainly rely on antibiotics to prevent infection, topical ointments to promote healing, or surgical intervention. However, these methods usually face problems such as limited efficacy, increased antibiotic resistance, insufficient local drug release, and recurrence of postoperative infection, and the patient's recovery process is long and painful.

[0076] To solve the above problems, the present embodiment provides a Chinese medicine spray film for promoting the healing of refractory diabetic wounds, wherein the formula is 1-10% of rhubarb extract, 0.5-5% of bletilla striata extract, 0.5-5% of notoginseng extract, 0.5-5% of liquorice extract, 2-10% of bletilla striata polysaccharide, 2-10% of polyvinyl pyrrolidone (PVP-K30), 0.1-5% of Tween-80 and 60-90% of water. Through reasonable ingredient ratio and scientific preparation process, the spray film has multiple functions of anti-inflammatory, hemostasis, anti-oxidation and promoting blood circulation.

[0077] The specific application scenario is a patient with diabetic foot ulcer, who has an ulcer with a diameter of about 2 cm on his foot, with redness around the ulcer and mild infection. Traditional antibiotic treatment is not effective, and due to long-term use of antibiotics, the patient has developed partial drug resistance, and the wound healing process is slow.

[0078] In this case, the doctor decided to use the Chinese medicine spray film in this embodiment for treatment. First, the wound was cleaned and disinfected to ensure that the bacteria and dirt on the surface were removed. Then, the prepared Chinese medicine spray film was evenly sprayed on the ulcer surface and covered with a layer of sterile gauze to protect the wound. The spray film was replaced once in the morning and evening every day for two weeks.

[0079] During the treatment process, the rhubarb and licorice extracts in the spray film quickly exerted anti-inflammatory and antibacterial effects, inhibiting infection at the wound, while the white radix and panax notoginseng extracts promoted blood circulation and accelerated tissue repair and regeneration. Because the spray film has good adhesion and sustained release effects, the drug ingredients can act on the ulcer surface for a long time and continue to exert therapeutic effects, which not only reduces the frequency of wound treatment, but also improves the effectiveness of treatment.

[0080] After two weeks, the patient's ulcer was significantly reduced, the surrounding skin returned to normal color, and the inflammation subsided. Compared with traditional treatment methods, the spray film showed a faster healing speed and lower infection recurrence rate, while avoiding the drug resistance problem caused by antibiotics. Through the application of this embodiment, the patient's wound healing time was shortened by about 30%, and the quality of life was significantly improved.

[0081] This embodiment fully demonstrates the unique advantages of the Chinese medicine spray film of the present invention in the treatment of refractory diabetic wounds, effectively solves the problems of insufficient efficacy and drug resistance in the prior art, achieves a significant improvement in the treatment effect, and provides a safer and more efficient treatment plan for diabetic patients.

[0082] Embodiment 2:

[0083] In the early stage of the treatment of diabetic refractory wounds, animal experiments are the key link to verify the efficacy of drugs. This example aims to verify the actual effect of the Chinese medicine spray film of the present invention in accelerating wound healing through diabetic rat model experiments.

[0084] First, male rats weighing 22 to 25 g were selected for adaptive feeding for 7 days, and then a diluted streptozotocin (STZ) solution was injected intraperitoneally to successfully induce the diabetic state. The standard for successful modeling was that the fasting blood glucose value of the rats was greater than 11.8 mmol / L. Finally, 16 rats met the standard and were included in the subsequent experiments.

[0085] After successful modeling, isoflurane was used for respiratory anesthesia, and a full-thickness skin defect with a diameter of 1 cm was prepared on the back of the rat to simulate a diabetic refractory wound. The rats were randomly divided into three groups, 8 in each group: normal rats as a blank group, diabetic rats treated with saline as a control group, and diabetic rats treated with the Chinese medicine spray film of the present invention as a treatment group. The spray film was evenly applied to the wound twice a day in the treatment group, and the control group was only wiped with saline.

[0086] On days 0, 2, 4, and 6 after treatment, the wound area of ​​the rats was photographed and measured, and the wound area was analyzed using professional software PS and ImageJ to calculate the wound healing rate. The calculation formula for the wound healing rate is: (initial wound area - area at measurement) / initial wound area × 100%.

[0087] The experimental results showed that the wound healing speed of the rats in the treatment group was significantly faster than that in the control group. On the 6th day of treatment, the wound healing rate of the treatment group was significantly higher than that of the control group, and the wound surface was almost healed. Through histological analysis, the wounds of the rats in the treatment group showed more complete epithelial healing, a large amount of new granulation tissue appeared in the dermis, and the inflammatory infiltration was significantly reduced, which shows that the Chinese medicine spray film of the present invention can effectively promote the healing of refractory wounds in diabetic rats.

[0088] This example fully demonstrates the excellent effect of the Chinese medicine spray film of the present invention in the treatment of diabetic refractory wounds. Compared with simple saline treatment or traditional treatment methods, the spray film can significantly accelerate wound healing, reduce infection risks, and improve the formation of new tissues through its multiple synergistic effects. This result provides a strong experimental basis for the potential of the present invention in clinical applications, and further highlights the innovation and practicality of the present invention.

[0089] Embodiment 3:

[0090] This example further verifies the effectiveness of the Chinese medicine spray film of the present invention in treating diabetic refractory wounds through histopathological analysis. The experiment uses a diabetic rat model, and simulates diabetic refractory wounds by preparing a full-thickness skin defect on the back. The treatment group is treated with the Chinese medicine spray film of the present invention.

[0091] During the experiment, the rats were divided into different experimental groups and treated separately, including the normal wound group (normal rats without modeling), the diabetic wound group (modeling but not treated) and the treatment group (using Chinese medicine spray after modeling). On the 6th day after treatment, the wounds of the rats in each group were sampled and HE stained to observe the healing and tissue repair of the wounds in each group.

[0092] HE staining results showed that the degree of epithelial healing in the wound area of ​​the normal wound group and the treatment group was significantly better than that of the diabetic wound group. In the treatment group, the epithelium in the injured area was basically healed, the new epithelial stratum spinosum thickened, the basal layer cells proliferated actively, and a large amount of granulation tissue composed of fibroblasts, new microvessels and red blood cells was formed in the upper dermis. In contrast, the epithelium in the injured area of ​​the diabetic wound group mostly disappeared, and the dermis was replaced by a large amount of inflammatory scabs and scar tissue, showing a lower degree of healing.

[0093] In addition, more collagen fibers and new microvessels were formed in the dermis of the treatment group, and inflammatory infiltration was significantly reduced, indicating that the Chinese medicine spray can not only accelerate epithelial healing, but also promote the repair and regeneration of the dermis. Compared with untreated diabetic wounds, the wounds in the treatment group showed a more complete and healthy healing state in terms of tissue structure.

[0094] The histological analysis of this example further confirmed the significant efficacy of the Chinese medicine spray film of the present invention in promoting the healing of diabetic refractory wounds, especially in epithelial repair, granulation tissue formation and inflammation control. These results show that the Chinese medicine spray film of the present invention can effectively overcome the healing barriers of diabetic refractory wounds and significantly improve the repair quality of wounds. The specific analysis is as follows:

[0095] See also Figure 1The structures of all layers of skin tissue are visible, and the inflammatory scabs are free. The epithelium is completely healed, a large number of skin appendages can be seen in the dermis, scar repair tissue can be seen in the upper dermis, and mixed inflammatory cell infiltration and muscle fiber necrosis can be seen locally in the subcutaneous muscle layer.

[0096] See also Figure 2 The epithelium in the injured area is basically healed, and the new epithelial stratum spinosum is thickened and the basal layer cells are actively proliferating. Granulation tissue composed of fibroblasts, red blood cells and new microvascular tissue can be seen in the upper dermis, and the skin appendages disappear. Scar tissue is gradually formed in the lower dermis, and the subcutaneous muscle layer disappears.

[0097] See also Figure 3 The epithelium in the injured area is basically healed, and the new epithelial acanthosis under the inflammatory scab can be seen, and the basal layer cells are actively proliferating. Granulation tissue composed of fibroblasts, red blood cells and new microvascular tissue can be seen in the upper dermis. Scar tissue can be seen gradually forming in the lower dermis, and the skin appendages and subcutaneous muscle layer disappear.

[0098] See also Figure 4 The epithelium in the damaged area disappears and is replaced by inflammatory scabs. Granulation tissue composed of fibroblasts, red blood cells and new microvessels can be seen in the upper dermis. Collagen fibers, new microvessels and a large number of mixed inflammatory cells can be seen in the lower dermis. The skin appendages disappear, and the muscle layer disappears.

[0099] See also Figure 5 The epithelium in the injured area is basically healed, the spinous layer is thickened, the basal layer cells proliferate actively, and free inflammatory scabs can be seen. Granulation tissue composed of a large number of red blood cells, fibroblasts and new microvessels can be seen in the papillary dermis. Scar tissue composed of a large number of collagen fibers and mixed inflammatory cells can be seen in the subdermis, a small amount of skin appendages can be seen at the edge, and the muscle layer disappears.

[0100] See also Figure 6 The epithelial tissue in the injured area showed a healing trend, and the new epithelium gradually increased in value. The dermis was replaced by granulation tissue, the skin appendages disappeared, and the subcutaneous muscle layer disappeared.

[0101] See also Figure 7 Most of the epithelium in the injured area disappears, and the newly formed epithelium at the edge is in a proliferative state. Inflammatory scabs can be seen covering the injured area. The dermis is replaced by granulation tissue, the skin appendages disappear, and the subcutaneous muscle layer disappears.

[0102] See also Figure 8 The epithelium in the injured area is basically healed, the inflammatory scab is free, and a large number of neutrophil infiltration and cystic lesions can be seen in the epithelium. The dermis is replaced by new granulation tissue, which contains a large number of red blood cells and fibroblasts, collagen fibers, and mixed inflammatory cells. The skin appendages disappear, and the muscle layer disappears.

[0103] See also Fig. 9The epithelium in the damaged skin area disappears, the dermis is replaced by granulation tissue, the skin appendages disappear, and the muscle layer disappears.

[0104] See also Fig.10 In the case of normal wounds, the epithelial structure of the skin injury area disappears and is replaced by an inflammatory scab. A large amount of granulation tissue can be seen under the scab, and mature granulation tissue, namely scar tissue, can be seen in the deep layer. The skin appendages disappear and the muscle layer disappears.

[0105] See also Fig.11 In the case of another normal wound, the epithelial structure of the skin injury area disappears and is replaced by an inflammatory scab. A large amount of granulation tissue can be seen under the scab, and mature granulation tissue, namely scar tissue, can be seen in the deep layer. The skin appendages disappear and the muscle layer disappears.

[0106] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

Claims

1. A Chinese medicine spray film for promoting the healing of refractory diabetic wounds, characterized in that: The Chinese medicine spray film comprises the following ingredients in percentage by weight: Rhubarb extract: 1-10%; Bletilla striata extract: 0.5-5%; Panax notoginseng extract: 0.5-5%; Licorice extract: 0.5-5%; Bletilla striata polysaccharide: 2-10%; Polyvinylpyrrolidone: 2-10%; Tween-80: 0.1-5%; Water: 60-90%.

2. The Chinese medicine film spray according to claim 1, characterized in that: The pH value of the traditional Chinese medicine spray film is between 4.0 and 7.

5.

3. The Chinese medicine film spray according to claim 1, characterized in that: The extracts of rhubarb, bletilla striata, notoginseng and liquorice are obtained by water extraction and alcohol precipitation method.

4. The Chinese medicine film spray according to claim 1, characterized in that: The method for using the spray film comprises: spraying the spray film uniformly on the surface of the diabetic refractory wound, applying 2 to 3 times a day until the wound is healed.

5. A method for treating diabetic refractory wounds, characterized in that: The method comprises applying the traditional Chinese medicine spray film according to any one of claims 1 to 4 to the surface of a refractory wound of a diabetic patient, applying it 2 to 3 times a day, and continuously using it until the wound is healed.

6. The method for treating diabetic refractory wounds according to claim 5, characterized in that: The method comprises the steps of thoroughly cleaning and disinfecting the wound before treatment, ensuring that the spray film evenly covers the wound surface, and avoiding wound contamination during the treatment process.

7. A method for preparing the Chinese medicine film spray according to claim 1, characterized in that: The preparation method comprises the following steps: Step 1, mixing raw materials of rhubarb, bletilla striata, notoginseng and liquorice in a weight ratio of 2:1:1:1, and soaking in water for 30 minutes; Step 2, heating the soaked mixture to boiling, turning to low heat and continuing to boil for 30 minutes, filtering to obtain the first decoction, repeating the process for the second decoction, and boiling the second decoction for 20 minutes; Step 3, combining the first decoction and the second decoction, and continuing to filter to obtain a clear liquid; Step 4, adding Bletilla striata polysaccharide and polyvinyl pyrrolidone to part of the clear solution at 50° C., and stirring until completely dissolved; Step 5, adding the crude powder of the combined drug to the solution obtained in step 4, continuing to stir evenly, cooling to room temperature, adding Tween-80, continuing to stir evenly, and diluting to the target volume with clear liquid to obtain a traditional Chinese medicine spray film.

8. The method for preparing the Chinese medicine film spray according to claim 7, characterized in that: In step 4, a magnetic stirrer is used for stirring, the mixture is heated at 50-60° C., and stirring is continued for 15-30 minutes until the mixture is completely dissolved.

9. The method for preparing the Chinese medicine film spray according to claim 7 or 8, characterized in that: The total decoction time of the clear liquid is 50 minutes, and the volume of the clear liquid after filtration is 2.5-3 times the weight of the initial raw material.

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