Anti-heparin interference uric acid detection kit as well as preparation method and application thereof

Abstract

The invention relates to the technical field of detection reagents, in particular to an anti-heparin interference uric acid detection kit as well as a preparation method and application thereof. The uric acid detection kit provided by the invention consists of an independently packaged reagent R1 and an independently packaged reagent R2, the reagent R1 is prepared from a buffer solution, 2-hydroxy-3-m-toluidine sodium propanesulfonate, disodium ethylene diamine tetraacetate, a preservative, ascorbic acid oxidase, bovine serum albumin and streptomycin sulfate; the reagent R2 is prepared from a buffer solution, uricase, ethylenediamine tetraacetic acid disodium salt, a preservative, peroxidase, 4-aminoantipyrine and bovine serum albumin. According to the uric acid detection kit provided by the invention, streptomycin sulfate is added and combined with bovine serum albumin, so that interference of heparin is effectively removed, enzyme decomposition and non-specific adsorption are prevented through BSA to enhance the stability of the reagent without influencing the stability of the reagent, and the problems that a uric acid reagent in the industry does not resist heparin and the homologous sample detection difference is relatively large are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of detection reagents, and in particular to a uric acid detection kit resistant to heparin interference, and a preparation method and application thereof. Background Art

[0002] Uric acid (UA) is a white crystalline substance that is difficult to dissolve in water, acid, ether and ethanol. It is weakly alkaline and can form salts with strong acids. The uricase method is usually used to determine uric acid. The uric acid level is assessed by detecting the uric acid concentration in urine or blood. This is one of the most commonly used methods. To facilitate testing, many hospitals currently add anticoagulants or coagulants to sample types. Heparin is one of the commonly used anticoagulants in disposable human arterial blood sample collectors. Sodium and lithium salts are common in clinical blood tests. However, heparin may react with components in the uric acid reagent, resulting in higher test results and larger deviations in testing homologous samples. Summary of the invention

[0003] In order to solve the above problems, the present invention provides a uric acid detection kit resistant to heparin interference and its preparation method and application. The uric acid detection kit provided by the present invention solves the problem of heparin interference in uric acid detection.

[0004] In order to achieve the above object, the present invention provides the following technical solutions: The present invention provides a uric acid detection kit resistant to heparin interference, which is composed of an independently packaged reagent R1 and an independently packaged reagent R2; The reagent R1 is composed of the following components: buffer solution 50-500 mmol / L, sodium 2-hydroxy-3-m-toluidine propanesulfonate 1 g / L, disodium ethylenediaminetetraacetate 1.2-1.5 g / L, preservative 0.5-2 g / L, ascorbic acid oxidase 6 kU / L, bovine serum albumin 1-2 g / L and streptomycin sulfate 0.5-1 g / L; the pH value of the reagent R1 is 6.5-7.0; The reagent R2 is composed of the following components: buffer solution 50-500 mmol / L, uricase 1 kU / L, disodium ethylenediaminetetraacetate 0.8-1 g / L, preservative 0.5-2 g / L, peroxidase 10 kU / L, 4-aminoantipyrine 0.3-0.5 g / L and bovine serum albumin 1.5-2 g / L; the pH value of the reagent R2 is 7.5-7.8.

[0005] Preferably, the reagent R1 is composed of the following components: buffer 50-100 mmol / L, 2-hydroxy-3-m-toluidine propanesulfonic acid sodium 1 g / L, ethylenediaminetetraacetic acid disodium 1.2-1.5 g / L, preservative 0.5 g / L, ascorbic acid oxidase 6 kU / L, bovine serum albumin 1.5-2 g / L and streptomycin sulfate 0.5-1 g / L; The reagent R2 is composed of the following components: buffer 50-100 mmol / L, uricase 1 kU / L, disodium ethylenediaminetetraacetate 0.8-1 g / L, preservative 0.5 g / L, peroxidase 10 kU / L, 4-aminoantipyrine 0.3-0.5 g / L and bovine serum albumin 1.5-2 g / L.

[0006] Preferably, the reagent R1 is composed of the following components: buffer 50 mmol / L, 2-hydroxy-3-m-toluidine propanesulfonic acid sodium 1 g / L, ethylenediaminetetraacetic acid disodium 1.5 g / L, preservative 0.5 g / L, ascorbic acid oxidase 6 kU / L, bovine serum albumin 2 g / L and streptomycin sulfate 1 g / L; The reagent R2 is composed of the following components: buffer 50 mmol / L, uricase 1 kU / L, disodium ethylenediaminetetraacetate 0.8 g / L, preservative 0.5 g / L, peroxidase 10 kU / L, 4-aminoantipyrine 0.5 g / L and bovine serum albumin 2 g / L.

[0007] Preferably, the pH value of the reagent R1 is 6.5; the pH value of the reagent R2 is 7.8.

[0008] Preferably, the buffer comprises MES buffer, Good's buffer or phosphate buffer.

[0009] Preferably, the preservative comprises sodium azide and / or Proclin 300.

[0010] Preferably, the volume ratio of the reagent R1 to the reagent R2 is 4:1.

[0011] The present invention provides a method for preparing the uric acid detection kit described in the above technical solution, comprising the following steps: Mixing a buffer solution, sodium 2-hydroxy-3-m-toluidinepropanesulfonate, disodium ethylenediaminetetraacetate, a preservative, ascorbic acid oxidase, bovine serum albumin and streptomycin sulfate in proportion, and adjusting the pH value of the mixture to 6.5-7.0 to obtain the reagent R1; The buffer solution, uricase, disodium ethylenediaminetetraacetate, preservative, peroxidase, 4-aminoantipyrine and bovine serum albumin are mixed in proportion and the pH value of the mixture is adjusted to 7.5-7.8 to obtain the reagent R2.

[0012] The present invention provides the use of the uric acid detection kit described in the above technical scheme or the uric acid detection kit prepared by the above preparation method in detecting uric acid, wherein the direct purpose of the detection is non-diagnosis and non-treatment.

[0013] Preferably, the sample to be tested includes a sample containing heparin.

[0014] Beneficial effects: The invention provides a uric acid detection kit for resisting heparin interference, which is composed of an independently packaged reagent R1 and an independently packaged reagent R2; the reagent R1 is composed of the following components: 50-500 mmol / L of buffer, 1 g / L of 2-hydroxy-3-m-toluidine propanesulfonic acid sodium, 1.2-1.5 g / L of disodium ethylenediaminetetraacetic acid, 0.5-2 g / L of preservative, 6 kU / L of ascorbic acid oxidase, 1-2 g / L of bovine serum albumin and 0.5-2 g / L of streptomycin sulfate. .5~1g / L; the pH value of the reagent R1 is 6.5~7.0; the reagent R2 is composed of the following components: buffer 50~500mmol / L, uricase 1kU / L, disodium ethylenediaminetetraacetate 0.8~1g / L, preservative 0.5~2g / L, peroxidase 10kU / L, 4-aminoantipyrine 0.3~0.5g / L and bovine serum albumin 1.5~2g / L; the pH value of the reagent R2 is 7.5~7.8. The uric acid detection kit provided by the present invention adds streptomycin sulfate and combines bovine serum albumin, effectively removing the interference of heparin, and preventing enzyme decomposition and nonspecific adsorption by bovine serum albumin to enhance the stability of the reagent without affecting it, solving the problem that the uric acid reagent in the industry is not resistant to heparin and the detection results of homologous samples have large deviations. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0016] Figure 1 The correlation results of the uric acid detection kit prepared in Example 1; Figure 2 The correlation results of the uric acid detection kit prepared in Example 2; Figure 3 The correlation results of the uric acid detection kit prepared in Comparative Example 1; Figure 4 This is the correlation result of the uric acid detection kit prepared in Comparative Example 3. DETAILED DESCRIPTION

[0017] The present invention provides a uric acid detection kit resistant to heparin interference, which is composed of an independently packaged reagent R1 and an independently packaged reagent R2; The reagent R1 is composed of the following components: buffer solution 50-500 mmol / L, sodium 2-hydroxy-3-m-toluidine propanesulfonate 1 g / L, disodium ethylenediaminetetraacetate 1.2-1.5 g / L, preservative 0.5-2 g / L, ascorbic acid oxidase 6 kU / L, bovine serum albumin 1-2 g / L and streptomycin sulfate 0.5-1 g / L; the pH value of the reagent R1 is 6.5-7.0; The reagent R2 is composed of the following components: buffer solution 50-500 mmol / L, uricase 1 kU / L, disodium ethylenediaminetetraacetate 0.8-1 g / L, preservative 0.5-2 g / L, peroxidase 10 kU / L, 4-aminoantipyrine 0.3-0.5 g / L and bovine serum albumin 1.5-2 g / L; the pH value of the reagent R2 is 7.5-7.8.

[0018] As one embodiment, the concentration of the buffer in the reagent R1 of the present invention is 50~100mmol / L; as another embodiment, the concentration of the buffer in the reagent R1 of the present invention is 50mmol / L; as one embodiment, the buffer in the reagent R1 includes MES buffer, Good's buffer or phosphate buffer; as another embodiment, the buffer in the reagent R1 is phosphate buffer.

[0019] As an embodiment, the concentration of 2-hydroxy-3-m-toluidine propanesulfonate sodium in the reagent R1 of the present invention is 1 g / L. The 2-hydroxy-3-m-toluidine propanesulfonate sodium of the present invention is used as a chromogenic substrate to produce a color change through an enzyme-catalyzed oxidative coupling reaction, thereby quantitatively detecting specific biochemical indicators.

[0020] As an embodiment, the concentration of disodium ethylenediaminetetraacetate in the reagent R1 of the present invention is 1.2-1.5 g / L; as another embodiment, the concentration of disodium ethylenediaminetetraacetate in the reagent R1 of the present invention is 1.5 g / L. The disodium ethylenediaminetetraacetate of the present invention is used as a stabilizer to maintain the stability of the product.

[0021] As an embodiment, the concentration of the preservative in the reagent R1 of the present invention is 0.5 g / L; as another embodiment, the preservative in the reagent R1 includes sodium azide and / or Proclin 300.

[0022] As an embodiment, the concentration of ascorbic acid oxidase in the reagent R1 of the present invention is 6 kU / L.

[0023] As an embodiment, the concentration of bovine serum albumin in the reagent R1 of the present invention is 1.5-2 g / L; as another embodiment, the concentration of bovine serum albumin in the reagent R1 of the present invention is 2 g / L. The bovine serum albumin of the present invention can prevent enzyme decomposition and nonspecific adsorption in the reagent R1, thereby enhancing the stability of the reagent R1 without affecting it.

[0024] As an embodiment, the concentration of streptomycin sulfate in the reagent R1 of the present invention is 1 g / L. The streptomycin sulfate of the present invention is an anti-interference agent, which can effectively remove the interference of heparin on the urea detection result.

[0025] As one embodiment, the concentration of the buffer in the reagent R2 of the present invention is 50~100mmol / L; as another embodiment, the concentration of the buffer in the reagent R2 of the present invention is 50mmol / L; as one embodiment, the buffer in the reagent R2 includes MES buffer, Good's buffer or phosphate buffer; as another embodiment, the buffer is phosphate buffer.

[0026] As an embodiment, the concentration of uricase in the reagent R2 of the present invention is 1 kU / L.

[0027] As an embodiment, the concentration of disodium ethylenediaminetetraacetate in the reagent R2 of the present invention is 0.8 g / L.

[0028] As an embodiment, the concentration of the preservative in the reagent R2 of the present invention is 0.5 g / L; as another embodiment, the preservative in the reagent R2 includes sodium azide and / or Proclin 300.

[0029] As an embodiment, the concentration of peroxidase in the reagent R2 of the present invention is 10 kU / L.

[0030] As an embodiment, the concentration of 4-aminoantipyrine in the reagent R2 of the present invention is 0.5 g / L. The 4-aminoantipyrine of the present invention is an important substance for the TOOS color development reaction.

[0031] As an embodiment, the concentration of serum albumin in the reagent R2 of the present invention is 2 g / L. The bovine serum albumin of the present invention can prevent enzyme decomposition and non-specific adsorption in the reagent R2, thereby enhancing the stability of the reagent R2 without affecting it.

[0032] As an embodiment, the pH value of the reagent R1 is 6.5; the pH value of the reagent R2 is 7.8.

[0033] As an embodiment, the volume ratio of the reagent R1 to the reagent R2 is 4:1.

[0034] The uric acid detection kit provided by the invention is an anti-heparin stable uric acid detection kit with stable substrate, accurate determination, convenient use and stable and reliable performance.

[0035] The present invention provides a method for preparing the uric acid detection kit described in the above technical solution, comprising the following steps: Mixing a buffer solution, sodium 2-hydroxy-3-m-toluidinepropanesulfonate, disodium ethylenediaminetetraacetate, a preservative, ascorbic acid oxidase, bovine serum albumin and streptomycin sulfate in proportion, and adjusting the pH value of the mixture to 6.5-7.0 to obtain the reagent R1; The buffer solution, uricase, disodium ethylenediaminetetraacetate, preservative, peroxidase, 4-aminoantipyrine and bovine serum albumin are mixed in proportion and the pH value of the mixture is adjusted to 7.5-7.8 to obtain the reagent R2.

[0036] Based on the above advantages, the present invention provides the use of the uric acid detection kit described in the above technical solution or the uric acid detection kit prepared by the above preparation method in detecting uric acid, wherein the direct purpose of the detection is non-diagnosis and non-treatment, and is only to obtain a detection value of uric acid in the sample. As an embodiment, the sample detected includes a sample containing heparin.

[0037] In order to further illustrate the present invention, a heparin-interference-resistant uric acid detection kit provided by the present invention, and its preparation method and application are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0038] Example 1 A uric acid (UA) detection kit with good stability, comprising an independently packaged reagent R1 and an independently packaged reagent R2, wherein the volume ratio of the reagent R1 to the reagent R2 is 4:1; The reagent R1 consists of the following components: Phosphate buffer 50mmol / L, 2-hydroxy-3-toluidine propanesulfonate sodium (TOOS) 1g / L, ethylenediaminetetraacetic acid disodium 1.2g / L, sodium azide 0.5g / L, ascorbate oxidase 6kU / L, bovine serum albumin (BSA) 1.5g / L and streptomycin sulfate 0.5g / L; the streptomycin sulfate was purchased from Shanghai TiChemicals Industrial Development Co., Ltd., CAS No. 3810-74-0; the pH value of the reagent R1 is 7.0; The reagent R2 consists of the following components: Phosphate buffer 50mmol / L, uricase 1kU / L, disodium ethylenediaminetetraacetate 1g / L, sodium azide 0.5g / L, peroxidase 10kU / L, 4-aminoantipyrine (4-AAP) 0.3g / L and BSA 1.5g / L; the pH value of the reagent R2 is 7.5.

[0039] Example 2 A uric acid detection kit with good stability, comprising an independently packaged reagent R1 and an independently packaged reagent R2, wherein the volume ratio of the reagent R1 to the reagent R2 is 4:1; The reagent R1 consists of the following components: Phosphate buffer 50mmol / L, TOOS 1g / L, disodium ethylenediaminetetraacetate 1.5g / L, sodium azide 0.5g / L, ascorbate oxidase 6kU / L, BSA 2g / L and streptomycin sulfate 1g / L; the streptomycin sulfate was purchased from Shanghai TCI Chemical Industry Development Co., Ltd., CAS No. 3810-74-0; the pH value of the reagent R1 is 6.5; The reagent R2 consists of the following components: Phosphate buffer 50mmol / L, uricase 1kU / L, disodium ethylenediaminetetraacetate 0.8g / L, sodium azide 0.5g / L, peroxidase 10kU / L, 4-AAP 0.5g / L and BSA 2g / L; the pH value of the reagent R2 is 7.8.

[0040] Comparative Example 1 A uric acid detection kit similar to Example 2, except that streptomycin sulfate is not added.

[0041] Comparative Example 2 A uric acid detection kit similar to Example 2, except that BSA is not added.

[0042] Comparative Example 3 A uric acid detection kit similar to Example 2, except that the concentration of 4-AAP is 0.1 g / L.

[0043] Comparative Example 4 A uric acid detection kit similar to Example 2, except that the pH of reagent R1 is adjusted to 6.5, and the pH of reagent R2 is adjusted to 7.0.

[0044] Comparative Example 5 A uric acid detection kit similar to Example 2, except that the concentration of streptomycin sulfate is 1.2 g / L.

[0045] Comparative Example 6 A uric acid detection kit similar to Example 2, except that the concentration of streptomycin sulfate is 1.5 g / L.

[0046] Application Example 1 Sample correlation: 50 fresh heparin serum (heparin tube collection) were collected from the hospital, numbered 1 to 50, and the uric acid detection kits of Example 1, Example 2, Comparative Example 1 and Comparative Example 3 were compared with the uric acid detection kits circulating on the market (as a control kit, purchased from Ningbo Meikang Biotechnology Co., Ltd., batch number: 231225101) to detect the uric acid content in samples 1 to 50. The instrument used was a fully automatic biochemical analyzer TBA-2000FR (manufacturer: Toshiba, Japan), and the detection parameters were set as follows: main wavelength 548nm, sub-wavelength 700nm, sample volume 4μl, reagent R1 volume 200μl, reagent R2 volume 50μl. The results are shown in Tables 1 and Figure 1~Figure 4 .

[0047] Table 1 Sample correlation test results (unit: mmol / L)

[0048] From the above results, it can be seen that Example 2 has a higher correlation with the control kit sample. From Example 1, Example 2 and Comparative Example 1, it can be seen that the addition of streptomycin sulfate effectively removes the interference of heparin, and the detection value is more accurate; from Example 1, Example 2 and Comparative Example 3, it can be seen that the detection value of 4-AAP is more accurate at an appropriate concentration.

[0049] Application Example 2 Precision experiment: Take a heparin serum sample of a high-uric acid inpatient from the hospital (recorded as a high-value sample), and a heparin serum sample of a low-uric acid person for daily physical examination (recorded as a low-value sample), and compare the uric acid test kits of Example 1, Example 2, Comparative Example 1, Comparative Example 2 and Comparative Example 4 with the uric acid test kits circulating on the market (as a control kit, purchased from Ningbo Meikang Biotechnology Co., Ltd.), test the high-value sample and the low-value sample respectively, and repeat the measurement 10 times, and obtain 10 measurement values ​​respectively, and calculate the mean, standard deviation and coefficient of variation of the measurement results. The instrument used is a fully automatic biochemical analyzer TBA-2000FR (manufacturer: Toshiba, Japan), and the detection parameters are set as follows: main wavelength 548nm, secondary wavelength 700nm, sample volume 4μl, reagent R1 volume 200μl, reagent R2 volume 50μl. The results are shown in Table 2.

[0050] Table 2 Sample measurement results (unit: mmol / L)

[0051] As shown in Table 2, the results of the kit of the present invention and the control kit can meet the industry standard coefficient of variation of <10%, and the addition of BSA and pH have an effect on the precision of the reagent. The kit prepared in Example 2 performs best.

[0052] Application Example 3 Heparin sample detection: In order to detect the uric acid detection kit of Example 2 of the present invention and the uric acid kit currently in circulation on the market (as a control kit, purchased from Ningbo Meikang Biotechnology Co., Ltd.) for the detection of heparin samples, the present invention collected 20 heparin serum samples (collected in heparin tubes, numbered 1 to 20 respectively), 20 common serum samples (without heparin, numbered 21 to 40 respectively) and 8 groups of heparin homologous samples from the hospital; the heparin homologous samples consisted of 8 groups of homologous serum samples and homologous heparin samples (collected in heparin tubes); the homologous serum samples were numbered as homologous serum 1 to homologous serum 8, and the homologous heparin samples were numbered as homologous heparin 1 to homologous heparin 8. Different samples were detected using Example 2, Comparative Example 1, Comparative Example 5, Comparative Example 6 and the control kit, respectively. The instrument used was a fully automatic biochemical analyzer TBA-2000FR (manufacturer: Toshiba, Japan), and the detection parameters were set as follows: main wavelength 548nm, sub-wavelength 700nm, sample volume 4μl, reagent R1 volume 200μl, reagent R2 volume 50μl. The results are shown in Table 3.

[0053] Table 3 Sample measurement results (unit: mmol / L)

[0054] As shown in Table 3, the kit of the present invention has basically no deviation in value when detecting heparin anticoagulant plasma, and has almost no effect on the result, which is close to the measured value of the Meikang kit. In contrast, when the kit of Comparative Example 1 treats heparin samples, the test results are increased to varying degrees and the measured values ​​of homologous samples are seriously high. It can be seen that the addition of streptomycin sulfate solves the problem that the uric acid reagent is not anti-heparin and the measured value deviation of homologous samples is large. Comparative Examples 5 and 6 are verified by concentration gradient relative to Example 2. From the analysis of the results, the increase in the concentration of streptomycin sulfate will have a counterproductive effect on the sample measured value. The result of Example 2 with a concentration of 1g / L is the best, which is closest to the result of the control kit.

[0055] In summary, the kit of the present invention solves the problem that the uric acid kit is not resistant to heparin and the measurement deviation of homologous samples is large to a large extent, provides convenience for clinical detection, and is worthy of further promotion.

[0056] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A uric acid detection kit resistant to heparin interference, characterized in that: It consists of an independently packaged reagent R1 and an independently packaged reagent R2; The reagent R1 is composed of the following components: buffer solution 50-500 mmol / L, sodium 2-hydroxy-3-m-toluidine propanesulfonate 1 g / L, disodium ethylenediaminetetraacetate 1.2-1.5 g / L, preservative 0.5-2 g / L, ascorbic acid oxidase 6 kU / L, bovine serum albumin 1-2 g / L and streptomycin sulfate 0.5-1 g / L; the pH value of the reagent R1 is 6.5-7.0; The reagent R2 is composed of the following components: buffer solution 50-500 mmol / L, uricase 1 kU / L, disodium ethylenediaminetetraacetate 0.8-1 g / L, preservative 0.5-2 g / L, peroxidase 10 kU / L, 4-aminoantipyrine 0.3-0.5 g / L and bovine serum albumin 1.5-2 g / L; the pH value of the reagent R2 is 7.5-7.

8.

2. The uric acid detection kit according to claim 1, characterized in that The reagent R1 is composed of the following components: buffer 50-100 mmol / L, 2-hydroxy-3-m-toluidine propanesulfonic acid sodium 1 g / L, ethylenediaminetetraacetic acid disodium 1.2-1.5 g / L, preservative 0.5 g / L, ascorbic acid oxidase 6 kU / L, bovine serum albumin 1.5-2 g / L and streptomycin sulfate 0.5-1 g / L; The reagent R2 is composed of the following components: buffer 50-100 mmol / L, uricase 1 kU / L, disodium ethylenediaminetetraacetate 0.8-1 g / L, preservative 0.5 g / L, peroxidase 10 kU / L, 4-aminoantipyrine 0.3-0.5 g / L and bovine serum albumin 1.5-2 g / L.

3. The uric acid detection kit according to claim 1, characterized in that: The reagent R1 consists of the following components Composition: buffer 50mmol / L, sodium 2-hydroxy-3-m-toluidine propanesulfonate 1g / L, disodium ethylenediaminetetraacetate 1.5g / L, preservative 0.5g / L, ascorbate oxidase 6kU / L, bovine serum albumin 2g / L and streptomycin sulfate 1g / L; The reagent R2 consists of the following components Composition: buffer 50mmol / L, uricase 1kU / L, disodium ethylenediaminetetraacetic acid 0.8g / L, preservative 0.5g / L, peroxidase 10kU / L, 4-aminoantipyrine 0.5g / L and bovine serum albumin 2g / L.

4. The uric acid detection kit according to any one of claims 1 to 3, characterized in that The pH value of the reagent R1 is 6.5; the pH value of the reagent R2 is 7.

8.

5. The uric acid detection kit according to any one of claims 1 to 3, characterized in that The buffer includes MES buffer, Good's buffer or phosphate buffer.

6. The uric acid detection kit according to any one of claims 1 to 3, characterized in that The preservatives include sodium azide and / or Proclin 300.

7. The uric acid detection kit according to any one of claims 1 to 3, characterized in that The volume ratio of the reagent R1 to the reagent R2 is 4:

1.

8. The method for preparing the uric acid detection kit according to any one of claims 1 to 7, characterized in that: The steps include: Mixing a buffer solution, sodium 2-hydroxy-3-m-toluidinepropanesulfonate, disodium ethylenediaminetetraacetate, a preservative, ascorbic acid oxidase, bovine serum albumin and streptomycin sulfate in proportion, and adjusting the pH value of the mixture to 6.5-7.0 to obtain the reagent R1; The buffer solution, uricase, disodium ethylenediaminetetraacetate, preservative, peroxidase, 4-aminoantipyrine and bovine serum albumin are mixed in proportion and the pH value of the mixture is adjusted to 7.5-7.8 to obtain the reagent R2.

9. Use of the uric acid detection kit according to any one of claims 1 to 7 or the uric acid detection kit prepared by the preparation method according to claim 8 in detecting uric acid, wherein the direct purpose of the detection is non-diagnosis and non-treatment.

10. The use according to claim 9, characterized in that: The sample to be tested includes a sample containing heparin.

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