Superlubricous hydrogel microspheres, methods of preparation and use in the treatment of osteoarthritis

Abstract

The application discloses a kind of superlubricity hydrogel microspheres, preparation method and application in the treatment of osteoarthritis, it is related to superlubricity hydrogel interface technical field, wherein, superlubricity hydrogel microspheres have core-shell structure, including core and shell;The core is sodium alginate hydrogel microsphere, and the shell is hydration layer.Sodium alginate hydrogel microsphere as core provides stable structural basis, and hydration layer as shell can keep moisture, thereby reducing friction during movement.The superlubricity hydrogel microspheres provided by the application have superlubricity, excellent biocompatibility and degradability, can provide buffer for interarticular cartilage friction, change sliding friction to rolling friction, reduce cartilage wear, and delay the progress of osteoarthritis.

Description

Technical Field

[0001] The present invention relates to the technical field of super-lubricating hydrogel interfaces, and in particular to super-lubricating hydrogel microspheres, a preparation method and application in the treatment of osteoarthritis. Background Art

[0002] Hydrogel microspheres (HMs) are micron-sized spherical hydrogels composed of three-dimensional cross-linked hydrophilic polymers with excellent injectability and biocompatibility. HMs can reduce friction at the sliding interface through a rolling mechanism like ball bearings, making them attractive candidates for biolubricants. However, continuous friction can cause wear or rupture on the surface of HMs, causing structural damage, which in turn affects their lubrication properties and stability. In addition, long-term friction can also cause the internal network structure of HMs to dissociate or deform, making the shape of the microspheres irregular, thereby affecting their overall performance. Moreover, conventional microfluidic technology requires repeated washing to remove grease from the surface of the microspheres during the preparation of hydrogel microspheres. The process is cumbersome and there is a potential risk of unclean washing, which is not conducive to in vivo application.

[0003] Effective biological lubrication between articular cartilage is crucial for maintaining the biological function of cartilage. When articular cartilage is damaged, its frictional properties decrease. Abnormal frictional mechanical stimulation activates TGF-β1 in the extracellular matrix of cartilage cells, initiating the fibrosis process of chondrocytes and ultimately leading to the continuous degeneration of articular cartilage. Summary of the Invention

[0004] The main purpose of the present invention is to propose a super-lubricating hydrogel microsphere, a preparation method and its application in the treatment of osteoarthritis, aiming to improve the abnormal friction force between cartilages and promote cartilage repair by exerting the synergistic effect of hydration lubrication and ball bearing lubrication.

[0005] To achieve the above objectives, the present invention proposes super-lubricating hydrogel microspheres, which have a core-shell structure, including a core and an outer shell; the core is a sodium alginate hydrogel microsphere, and the outer shell is a hydration layer.

[0006] In one embodiment, the hydration layer is a poly-(2-methacryloyloxyethyl phosphorylcholine) lubricious coating.

[0007] In one embodiment, the poly-(2-methacryloyloxyethyl phosphorylcholine) lubricating coating has a thickness of 3-5 μm.

[0008] The present invention also provides a method for preparing the super-lubricating hydrogel microspheres, comprising the following steps:

[0009] S10, providing a sodium alginate aqueous solution and a calcium chloride aqueous solution;

[0010] S20, spraying the sodium alginate aqueous solution onto the calcium chloride aqueous solution by electrostatic spraying technology to obtain hydrogel microspheres;

[0011] S30, dissolving the hydrogel microspheres in a photoinitiator solution, shaking, and reacting to obtain an intermediate product;

[0012] S40, dispersing the intermediate product in a 2-methacryloyloxyethyl phosphorylcholine (MPC) solution, shaking and irradiating with ultraviolet light, collecting and washing to obtain the super-lubricating hydrogel microspheres.

[0013] In one embodiment, the mass concentration of sodium alginate in the sodium alginate aqueous solution in step S10 is 1-3 wt %; and / or,

[0014] The mass concentration of calcium chloride in the calcium chloride aqueous solution is 3-6wt%.

[0015] In one embodiment, the parameters of the electrostatic spray technology used in step S20 are as follows: positive high voltage is +0.5 to +5 kV, negative high voltage is -5 to -15 kV, and the stainless steel needle used is of 21-30G.

[0016] In one embodiment, the solute of the photoinitiator solution in step S30 includes one of 2-hydroxy-4-(2-hydroxyethoxy)-2-methylpropiophenone (I2959), lithium phenyl (2,4,6-trimethylbenzoyl) phosphate (LAP), acyl phosphate, alkyl aromatic ketone derivatives, thioxanthone derivatives and benzophenone; and / or,

[0017] The mass concentration of the solute is 1-3 wt%; and / or,

[0018] The shaking time is 0.5-2h.

[0019] In one embodiment, in step S40:

[0020] The mass concentration of the 2-methacryloyloxyethyl phosphorylcholine solution is 1-3wt%; and / or,

[0021] The shaking time is 0.5-2h.

[0022] In one embodiment, the power of the ultraviolet light irradiation in step S40 is 15-60W; and / or,

[0023] The wavelength of the ultraviolet light irradiation is 365-405 nm; and / or,

[0024] The duration of the ultraviolet light irradiation is 0.5-2h.

[0025] The present invention also provides a use of super-lubricating hydrogel microspheres in the treatment of osteoarthritis, including the super-lubricating hydrogel microspheres or the super-lubricating hydrogel microspheres prepared by the preparation method of the super-lubricating hydrogel microspheres.

[0026] The super-lubricating hydrogel microspheres provided by the present invention have a core-shell structure, including a core and an outer shell; the core is a sodium alginate hydrogel microsphere, and the outer shell is a hydration layer. The hydration layer formed by the interaction of water molecules with charged ions or zwitterions can greatly reduce friction. Adding a hydration layer to the surface of HMs can achieve a synergistic effect of hydration lubrication and ball bearing lubrication, expanding the application prospects of HMs in the field of biolubrication. Through injection therapy of super-lubricating hydrogel microspheres, super-lubricating hydrogel microspheres can maintain low friction mechanical stimulation between articular cartilage, physically shield the progression of chondrocyte fibrosis, and thus maintain the cartilage phenotype. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0028] Figure 1 A flow chart of the method for preparing super-lubricating hydrogel microspheres provided by the present invention;

[0029] Figure 2 This is a light microscopy image of the superlubricating hydrogel microspheres in Example 1 of the present invention;

[0030] Figure 3 Graph showing the change in coefficient of friction (COF) over time during the friction process of superlubricating hydrogel microspheres in Example 1 of the present invention;

[0031] Figure 4 This is a live-dead staining image of cells after co-incubation of super-lubricating hydrogel microspheres with cells in Example 1 of the present invention;

[0032] Figure 5 This is a visual representation of the superlubricating hydrogel microspheres in Example 1 of the present invention after degradation in pure water for 14 days;

[0033] Figure 6 This is a light microscopy image of the superlubricating hydrogel microspheres in Example 2 of the present invention;

[0034] Figure 7 This is a fluorescent staining image of the super-lubricating hydrogel microspheres in Example 2 of the present invention, wherein: Figure 7 (a) before dyeing, Figure 7 (b) after dyeing;

[0035] Figure 8 Fourier infrared spectra of the superlubricating hydrogel microspheres provided in Example 1 of the present invention and the hydrogel microspheres provided in Comparative Example 1;

[0036] Figure 9 This is a comparison chart of the coefficient of friction (COF) of the superlubricating hydrogel microspheres provided in Example 1 of the present invention and the hydrogel microspheres provided in Comparative Example 1.

[0037] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0039] It should be noted that if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited to "first" and "second" may explicitly or implicitly include at least one of such features. In addition, if "and / or" or "and / or" appears in the full text, its meaning includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme in which A and B are satisfied at the same time. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0040] Long-term friction can cause the internal network structure of HMs to dissociate or deform, making the microspheres irregular in shape and affecting their overall performance. The present invention uses electrostatic spraying technology to prepare hydrogel microspheres and perform surface modification to enhance their anti-friction properties and potential for application in tissue repair in vivo.

[0041] The present invention proposes super-lubricating hydrogel microspheres with a core-shell structure, comprising a core and an outer shell. The core is a sodium alginate hydrogel microsphere, and the outer shell is a hydration layer. The core provides a stable structural foundation, while the hydration layer acts as an outer shell, trapping water molecules in the surrounding environment and reducing friction during movement.

[0042] In one embodiment of the present invention, the hydration layer is a poly-(2-methacryloyloxyethyl phosphorylcholine) lubricating coating. This polymer chain contains a large number of hydrophilic groups that can tightly bind to water molecules, forming a thin hydration layer. The water molecules in the hydration layer can move relatively freely, giving the hydration layer a certain degree of fluidity and adaptability. This dynamic structure helps to continuously replenish the lubricating layer during friction, maintaining its lubrication properties.

[0043] In one embodiment of the present invention, the thickness of the poly (2-methacryloyloxyethyl phosphorylcholine) lubricating coating is 3-5 μm. The lubricating coating of this thickness can fully exert the hydration lubricating effect of PMPC, and a slight increase in size will not cause changes in the structure and performance of the hydrogel microspheres themselves.

[0044] The present invention also proposes a method for preparing the super-lubricating hydrogel microspheres. Since the preparation method adopts all the technical solutions of all the above embodiments, it has at least all the beneficial effects brought by the technical solutions of the above embodiments, which will not be described in detail here. Figure 1 As shown, the preparation method comprises the following steps:

[0045] S10, providing a sodium alginate aqueous solution and a calcium chloride aqueous solution;

[0046] S20, spraying the sodium alginate aqueous solution onto the calcium chloride aqueous solution by electrostatic spraying technology to obtain hydrogel microspheres;

[0047] S30, dissolving the hydrogel microspheres in a photoinitiator solution, shaking, and reacting to obtain an intermediate product;

[0048] S40, dispersing the intermediate product in a 2-methacryloyloxyethyl phosphorylcholine solution, shaking and irradiating with ultraviolet light, collecting and washing to obtain the super-lubricating hydrogel microspheres.

[0049] The sodium alginate aqueous solution serves as the hydrogel precursor solution, while the calcium chloride aqueous solution serves as the crosslinker solution. The calcium ions in the calcium chloride aqueous solution react with the carboxylic acid groups in the sodium alginate to form a gel. Electrostatic spraying technology efficiently produces uniform hydrogel microspheres. By oscillating the reaction in a photoinitiator solution, specific chemical molecules and functional groups can be introduced, enhancing the physical and chemical properties of the hydrogel microspheres.

[0050] The introduction of 2-methacryloyloxyethyl phosphorylcholine increases the hydrophilicity of the intermediate product's surface, enabling it to better interact with water molecules during movement. This enhanced hydrophilicity allows water molecules to form a hydration layer on the hydrogel surface, reducing direct contact between the superlubricating hydrogel microspheres and thus lowering the coefficient of friction. Ultraviolet light can excite the chemical bonds in the 2-methacryloyloxyethyl phosphorylcholine molecules, making them more active and prone to reacting with the intermediate product.

[0051] In one embodiment of the present invention, the mass concentration of sodium alginate in the sodium alginate aqueous solution in step S10 is 1-3 wt %. Within this concentration range, sodium alginate can provide a suitable viscosity and stability.

[0052] The calcium chloride solution has a calcium chloride concentration of 3-6 wt%. A higher calcium chloride concentration can accelerate the crosslinking rate, but too high a concentration may lead to excessive crosslinking, affecting the flexibility and mechanical properties of the superlubricating hydrogel microspheres. Within this range, the flexibility and mechanical properties of the superlubricating hydrogel microspheres can be improved while maintaining the crosslinking rate.

[0053] In one embodiment of the present invention, the electrostatic spraying technique used in step S20 has the following parameters: a positive high voltage of +0.5 to +5 kV, a negative high voltage of -5 to -15 kV, and a 21-30 gauge stainless steel needle. Electrostatic spraying is equivalent to applying an electric charge to the sodium alginate. Under the influence of the high-voltage electric field, the sprayed droplets repel each other, helping to form smaller microspheres. The high applied voltage difference promotes a stable spray effect, and the small needle diameter produces fine droplets.

[0054] In one embodiment of the present invention, the solute of the photoinitiator solution described in step S30 includes one of 2-hydroxy-4-(2-hydroxyethoxy)-2-methylpropiophenone, phenyl (2,4,6-trimethylbenzoyl) phosphate lithium salt, acyl phosphate, alkyl aromatic ketone derivative, thioxanthone derivative and benzophenone. The photoinitiator can generate free radicals under the irradiation of light of a specific wavelength, and these free radicals can trigger the cross-linking reaction of 2-methacryloyloxyethyl phosphorylcholine (MPC) monomer on the surface of the hydrogel microspheres. By controlling the intensity and time of the light irradiation, the degree and speed of the cross-linking can be precisely controlled. 2-hydroxy-4-(2-hydroxyethoxy)-2-methylpropiophenone has good solubility and high photosensitivity, and can effectively generate free radicals under ultraviolet irradiation, thereby initiating the polymerization of MPC monomers to form PMPC modified on the surface of the hydrogel microspheres. Phenyl (2,4,6-trimethylbenzoyl) phosphate lithium salt is suitable for monomer molecules requiring higher cross-linking strength and heat resistance as well as polymerization reactions for materials that need to be used for in vivo applications due to its excellent thermal stability, photosensitivity and low cytotoxicity.

[0055] In one embodiment of the present invention, the mass concentration of the solute in step S30 is 1-3 wt %. Within this concentration range, the photoinitiator can enter the hydrogel microspheres and accumulate on the surface of the microspheres by virtue of the hydrogel microspheres' swelling properties.

[0056] In one embodiment of the present invention, the shaking time in step S30 is 0.5-2 hours. Shaking ensures uniform contact between the hydrogel microspheres and the photoinitiator, thereby promoting the chemical reaction and generating the target intermediate product. Within this shaking time range, the photoinitiator is evenly mixed into the generated intermediate product.

[0057] In one embodiment of the present invention, the mass concentration of the 2-methacryloyloxyethyl phosphorylcholine solution in step S40 is 1-3 wt %. Within this range, the hydrophilicity of the intermediate product surface is enhanced, enabling the intermediate product to better interact with water molecules during movement. The water molecules can form a hydration layer on the hydrogel surface, reducing direct contact between the superlubricating hydrogel microspheres and thereby reducing the friction coefficient.

[0058] In one embodiment of the present invention, the shaking time in step S40 is 0.5-2 hours. Within this time range, the intermediate product is evenly dispersed in the 2-methacryloyloxyethyl phosphorylcholine solution.

[0059] In one embodiment of the present invention, the power of the ultraviolet light irradiation in step S40 is 15-60 W. Under this power, the hydrogel microspheres are not affected by high temperature and can maintain a good morphology.

[0060] In one embodiment of the present invention, the wavelength of the ultraviolet light irradiation in step S40 is 365-405 nm, which belongs to UVA (long-wave ultraviolet light) and can polymerize MPC monomers to form PMPC super-lubricating coatings.

[0061] In one embodiment of the present invention, the duration of the ultraviolet irradiation in step S40 is 0.5-2 hours. After 0.5-2 hours of ultraviolet irradiation, MPC polymerizes under the action of the photoinitiator and ultraviolet irradiation and forms a PMPC super-lubricating coating on the surface of the hydrogel microspheres.

[0062] The present invention also provides a use of superlubricating hydrogel microspheres for the treatment of osteoarthritis, including the superlubricating hydrogel microspheres described above or the superlubricating hydrogel microspheres prepared by the method for preparing superlubricating hydrogel microspheres described above. Because this application utilizes all of the technical solutions of all of the aforementioned embodiments, it at least possesses all of the beneficial effects brought about by the technical solutions of the aforementioned embodiments, and no further details will be given here.

[0063] The formed PMPC superlubricating coating (i.e., hydration layer) can greatly reduce friction. Therefore, adding a hydration layer to HMs can achieve the synergistic effect of hydration lubrication and ball bearing lubrication, expanding the application of HMs in the field of osteoarthritis treatment.

[0064] Example 1

[0065] This embodiment provides a method for preparing super-lubricating hydrogel microspheres, comprising the following steps:

[0066] S10, dissolving 200 mg of sodium alginate in 10 mL of deionized water to prepare a 2 wt % sodium alginate aqueous solution, and dissolving 3 g of calcium chloride in 100 mL of deionized water to prepare a 3 wt % calcium chloride aqueous solution;

[0067] S20, draw 10 mL of the sodium alginate aqueous solution with a 10 mL syringe and place it on the pusher, use a 30G (inner diameter 150 μm) stainless steel needle as a nozzle; connect the positive and negative poles of the power supply, and set the voltage to +2 kV for positive high voltage and -10 kV for negative high voltage; use a stainless steel container as a receiver, and add 100 mL of the calcium chloride solution thereto; then use electrostatic spray technology to cross-link the sodium alginate aqueous solution in the calcium chloride solution to prepare hydrogel microspheres, and set the distance between the nozzle and the receiver to 15 cm;

[0068] S30, dissolving 2 g of photoinitiator I2959 in 100 mL of deionized water to prepare a 2 wt % photoinitiator solution, dispersing the hydrogel microspheres into the photoinitiator solution, shaking, and reacting to obtain an intermediate product;

[0069] S40, dissolving 1g of MPC in 100mL of deionized water to prepare a 1% MPC solution, dispersing the intermediate product in the MPC solution, shaking for 2h and irradiating with ultraviolet light, collecting and washing to obtain the super-lubricating hydrogel microspheres. The ultraviolet light has a power of 15W, a wavelength of 365nm, and an irradiation time of 2h. Under the action of photoinitiator I2959 and ultraviolet light irradiation, MPC polymerizes and forms a PMPC super-lubricating coating on the surface of the intermediate product. The microspheres are collected and washed with pure water to obtain super-lubricating hydrogel microspheres.

[0070] The super-lubricating hydrogel microspheres obtained in this embodiment are regular spherical in shape and have a diameter of 150-200 μm (e.g. Figure 2 As shown). Figure 3 As shown in the figure, the superlubricating hydrogel microspheres have excellent lubricating properties. The friction coefficient of the superlubricating hydrogel microspheres is stable and low during long-term friction in the friction and wear tester. Figure 4As shown in the results, the superlubricating hydrogel microspheres have excellent biocompatibility and can support cell adhesion and proliferation. At the same time, the superlubricating hydrogel microspheres have suitable degradability and can be completely degraded within 14 days in a pure water environment ( Figure 5 ).

[0071] Example 2

[0072] Compared with Example 1, the difference is that in step S30, 1 g of photoinitiator LAP is dissolved in 100 mL of deionized water to prepare a 1 wt% photoinitiator solution.

[0073] The super-lubricating hydrogel microspheres obtained in this embodiment are regular spherical in shape and have a diameter of 150-200 μm (e.g. Figure 6 shown).

[0074] from Figure 7 (a) and from Figure 7 As can be seen in (b), after the super-lubricating hydrogel microspheres are stained with DiI, they can be efficiently combined with the poly-(2-methacryloyloxyethyl phosphorylcholine) lubricating coating PMPC to emit red fluorescence. It can be observed that the thickness of the poly-(2-methacryloyloxyethyl phosphorylcholine) lubricating coating on the surface of the super-lubricating hydrogel microspheres is about 3-5 μm.

[0075] Comparative Example 1

[0076] This comparative example provides a method for preparing hydrogel microspheres, comprising the following steps:

[0077] 200mg of sodium alginate was dissolved in 10mL of deionized water to prepare a 2wt% sodium alginate aqueous solution, and 3g of calcium chloride was dissolved in 100mL of deionized water to prepare a 3wt% calcium chloride aqueous solution. 10mL of the sodium alginate aqueous solution was drawn up with a 10mL syringe and placed on a push syringe. A 30G stainless steel needle (150μm inner diameter) was used as the nozzle. The positive and negative poles of the power supply were connected, and the voltage was set to +2kV for positive and -10kV for negative. A stainless steel container was used as a receiver, and 100mL of calcium chloride solution was added thereto. The sodium alginate aqueous solution was then cross-linked in the calcium chloride aqueous solution using electrostatic spraying technology to prepare hydrogel microspheres. The distance between the nozzle and the receiver was set to 15cm.

[0078] pass Figure 8 The results of Fourier transform infrared spectroscopy show that a specific absorption peak of MPC (1120 cm -1 , 1500cm -1 , 1700cm -1), indicating that MPC polymerized on the surface of the hydrogel microspheres to form a PMPC lubricating coating; while no specific absorption peak of MPC was detected on the surface of the hydrogel microspheres obtained in Comparative Example 1.

[0079] like Figure 9 As shown, the surface friction coefficient of the superlubricating hydrogel microspheres prepared in Example 1 and the surface friction coefficient of the hydrogel microspheres prepared in Comparative Example 1 were tested by a friction and wear testing machine. It can be seen that the surface friction coefficient of the hydrogel microspheres prepared in Comparative Example 1 is significantly higher than the surface friction coefficient of the superlubricating hydrogel microspheres prepared in Example 1.

[0080] In Examples 1 and 2, the hydrogel microspheres were further modified through photopolymerization to prepare superlubricating hydrogel microspheres for the treatment of osteoarthritis. While the hydrogel microspheres in Comparative Example 1 can also be used for osteoarthritis treatment, they only function to convert sliding friction into rolling friction and lack the inherent superlubricating coating of the hydrogel microspheres. Therefore, friction with cartilage can easily damage the microspheres, leading to structural breakdown and loss of their original functionality.

[0081] The above description is merely an exemplary embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformation made by utilizing the contents of the present invention's description and drawings under the technical concept of the present invention, or directly / indirectly applied in other related technical fields, is included in the patent protection scope of the present invention.

Claims

1. A super lubricating hydrogel microsphere, characterized in that: The super-lubricating hydrogel microspheres have a core-shell structure, including a core and an outer shell; the core is a sodium alginate hydrogel microsphere, and the outer shell is a hydration layer; The method for preparing the super-lubricating hydrogel microspheres is characterized by comprising the following steps: S10, providing a sodium alginate aqueous solution and a calcium chloride aqueous solution; S20, spraying the sodium alginate aqueous solution onto the calcium chloride aqueous solution by electrostatic spraying technology to obtain hydrogel microspheres; S30, dispersing the hydrogel microspheres in a photoinitiator solution, shaking, and reacting to obtain an intermediate product; S40, dispersing the intermediate product in a 2-methacryloyloxyethyl phosphorylcholine solution, shaking and irradiating with ultraviolet light, collecting and washing to obtain the super-lubricating hydrogel microspheres; The solute of the photoinitiator solution in step S30 is selected from one of 2-hydroxy-4-(2-hydroxyethoxy)-2-methylpropiophenone, phenyl (2,4,6-trimethylbenzoyl) phosphate lithium salt, thioxanthone derivatives and benzophenone; The hydration layer is a poly-(2-methacryloyloxyethyl phosphorylcholine) lubricating coating.

2. The super lubricating hydrogel microspheres according to claim 1, wherein The thickness of the poly-(2-methacryloyloxyethyl phosphorylcholine) lubricating coating is 3-5 μm.

3. The super lubricating hydrogel microspheres according to claim 1, wherein The mass concentration of sodium alginate in the sodium alginate aqueous solution in step S10 is 1-3 wt %; and / or, The mass concentration of calcium chloride in the calcium chloride aqueous solution is 3-6 wt%.

4. The super lubricating hydrogel microspheres according to claim 1, wherein The parameters of the electrostatic spray technology used in step S20 are as follows: positive high voltage is +0.5 to +5 kV, negative high voltage is -5 to -15 kV, and the stainless steel needle used is of 21-30 G.

5. The super lubricating hydrogel microspheres according to claim 1, wherein The mass concentration of the solute in step S30 is 1-3 wt%; and / or, The shaking time is 0.5-2 h.

6. The super lubricating hydrogel microspheres according to claim 1, wherein In step S40: The mass concentration of the 2-methacryloyloxyethyl phosphorylcholine solution is 1-3 wt%; and / or, The shaking time is 0.5-2 h.

7. The super lubricating hydrogel microspheres according to claim 1, wherein In step S40: The power of the ultraviolet light irradiation is 15-60 W; and / or, The wavelength of the ultraviolet light irradiation is 365-405 nm; and / or, The duration of the ultraviolet light irradiation is 0.5-2 h.

8. Use of the super-lubricating hydrogel microspheres according to any one of claims 1 to 7 in the preparation of a medicament for treating osteoarthritis.

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