A kind of high-efficiency liquid organic fertilizer and preparation method thereof

Abstract

The invention discloses a high-efficiency liquid organic fertilizer and a preparation method thereof, and belongs to the technical field of organic fertilizer. It is made of the following raw materials in parts by weight: 200-300 parts of livestock and aquaculture wastewater, 50-80 parts of plant straw, 10-20 parts of pretreatment agent, 10-20 parts of EM bacteria, 20-30 parts of composite microbial agent, 3-10 parts of promoter, 1-3 parts of potassium dihydrogen phosphate, and 0.05-0.2 parts of trace elements; the promoter is composed of sunflower disk extract and sodium lauryl alcohol polyether sulfate in a mass ratio of 3:1; the pretreatment agent is a biochar-TiO2 composite material. The present invention realizes the resource utilization of livestock and aquaculture wastewater, converts wastewater into liquid organic fertilizer rich in nutrient elements, provides high-quality fertilizer resources for agricultural production, effectively removes harmful substances in wastewater through the combination of biological treatment and physical and chemical treatment technology, ensures the safety and environmental protection of organic fertilizer, and the obtained organic liquid fertilizer has a significant effect on improving potato yield and quality, and has good application prospects.

Description

Technical Field

[0001] The invention belongs to the technical field of organic fertilizers, and in particular relates to a high-efficiency liquid organic fertilizer and a preparation method thereof. Background Art

[0002] As the scale of China's animal husbandry expands, the total amount of waste generated during the breeding process is increasing day by day. Among them, wastewater containing high concentrations of ammonia nitrogen, total nitrogen, and total phosphorus pollutants has a greater negative impact on the ecological environment. Animal husbandry wastewater mainly comes from urine, feces, feed residues, flushing water during flushing, etc. excreted by animals. According to the source and nature of wastewater, it can be divided into the following categories: 1. Fecal wastewater: produced by animal feces, containing a large amount of organic matter, nitrogen, phosphorus, potassium and other nutrients; 2. Production sewage: including wastewater generated during feed processing, animal washing, equipment washing, etc., containing certain organic matter and suspended matter; 3. Domestic sewage: wastewater generated by the daily life of farm staff; 4. Rainwater: Rainwater in the farm may be contaminated by pollutants such as feces and feed residues, and contains certain suspended matter and organic matter. Animal husbandry wastewater has typical "three high" characteristics, namely high organic matter concentration, high ammonia nitrogen content, and high suspended matter. In recent years, livestock breeding wastewater has become one of the main sources of water pollution emissions in my country, and livestock and poultry breeding waste management has also become a top priority in the development of the livestock and poultry industry.

[0003] Livestock wastewater contains a large amount of organic matter, nitrogen, phosphorus, potassium and other nutrients required for plant growth, but also contains harmful microorganisms, heavy metals and other potential pollutants. Traditional treatment methods often make it difficult to simultaneously achieve wastewater purification and effective resource utilization. Therefore, it is of great significance to develop a method that can convert livestock wastewater into safe and efficient liquid organic fertilizer. Summary of the invention

[0004] The object of the present invention is to provide a high-efficiency liquid organic fertilizer. The liquid organic fertilizer takes animal husbandry wastewater as a main raw material, removes some organic matter and heavy metal ions that are difficult to convert and utilize after pretreatment by adding a pretreatment agent, kills harmful bacteria, and makes the pretreated liquid provide more suitable conditions for subsequent microbial fermentation and growth, thereby preparing a high-efficiency liquid organic fertilizer.

[0005] In order to achieve the above technical objectives, the technical solution adopted by the present invention is:

[0006] A highly efficient liquid organic fertilizer is prepared from the following raw materials in parts by weight: 200-300 parts of livestock breeding wastewater, 50-80 parts of plant straw, 10-20 parts of pretreatment agent, 10-20 parts of EM bacteria, 20-30 parts of composite microbial agent, 3-10 parts of promoter, 1-3 parts of potassium dihydrogen phosphate, and 0.05-0.2 parts of trace elements; the promoter is composed of sunflower disc extract and sodium lauryl polyether sulfate in a mass ratio of 3:1; the pretreatment agent is a biochar-TiO2 composite material.

[0007] Preferably, the biochar-TiO2 composite material is prepared by the following method:

[0008] (1) Take 20 mL of anhydrous ethanol, add 10 mL of tetrabutyl titanate and 1.5 mL of glacial acetic acid in sequence under vigorous stirring, and stir for 30 min to obtain a mixed solution A;

[0009] (2) Take 20 mL of anhydrous ethanol, add 2.5 mL of deionized water to obtain an alcohol-water mixed solution, take 0.45 g of manganese acetate and add it to the above alcohol-water mixed solution, add 0.2 mL of nitric acid dropwise, and stir for 20 min to obtain a mixed solution B;

[0010] (3) Add mixed solution B drop by drop into mixed solution A and continue stirring for 10 min. After aging at room temperature for 6-8 h, a gel is obtained. The gel is placed in an oven, dried at 80 °C, ground into powder, and calcined at 500 °C for 2 h to obtain Mn-doped nano-TiO2 material.

[0011] (4) Take 5 g of rice husk biochar and soak it in 50 ml of 5 mol / L potassium hydroxide solution for 2 h, then take it out and dry it for later use;

[0012] (5) Take 1 g of the Mn-doped nano-TiO2 material obtained in step (3), add it to 100 ml of 2.5 mol / L NaOH solution, and sonicate for 10 min to obtain a nano-TiO2 dispersion;

[0013] (6) Take 5 g of the rice husk biochar treated with alkali in step (4), disperse it in 500 ml of deionized water, then add 20 ml of the nano-TiO2 dispersion obtained in step (5) thereto, ultrasonically treat for 10-20 min, and then react with magnetic stirring for 4 h. The reaction solution is centrifuged, filtered, washed, and dried to obtain a solid product. After vacuum drying at 75°C for 10 h, the solid product is transferred to a tubular furnace and heat treated at 400°C in a nitrogen atmosphere for 2 h to obtain a biochar-TiO2 composite material.

[0014] Preferably, the preparation method of the sunflower disc extract is as follows: take a sunflower disc, clean it and dry it in an oven at 80°C for 2 hours, crush it through an 80-mesh sieve to obtain sunflower disc powder, then add a complex enzyme solution for enzymatic hydrolysis for 8-10 hours, the enzymatic hydrolysis temperature is 45-50°C, inactivate it at high temperature, and then cool it to room temperature to obtain the sunflower disc extract.

[0015] Preferably, the material-liquid ratio of the sunflower disk powder to the complex enzyme solution is 1g:10ml.

[0016] Preferably, the complex enzyme solution contains 10-15 g / L of papain and 15-20 g / L of cellulase.

[0017] Preferably, the composite microbial agent is prepared by the following method: thawing and activating Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streaking the plates and culturing them in an incubator at 28°C for 48 hours, taking a loop of the cultured strains into LB liquid culture medium, placing it in a shaker at 28°C and 200 r / min for 24 hours to obtain seed liquid; inoculating the seed liquids of the four strains into NB liquid culture medium at an inoculation rate of 3%, bottling 200 mL, 30°C, and 200 r / min for 72 hours to obtain fermentation liquid; mixing the four fermentation liquids in a mass ratio of 1:1:1:1 to obtain a composite microbial agent.

[0018] The Bacillus licheniformis strain is numbered as CGMCC No.1.10257, purchased from China General Microorganism Culture Collection Administration Center, and the preservation date is November 4, 2009; the Bacillus subtilis strain is numbered as CGMCC No.1.7740, purchased from China General Microorganism Culture Collection Administration Center, and the preservation date is July 24, 2008; the Pseudomonas chlororaphis strain is numbered as CGMCC No.1.2785, and the preservation date is July 17, 2001; the Trichoderma viride strain is numbered as CGMCC No.3.7338, and the preservation date is July 8, 2005. The Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride used in the present invention can be purchased through the strain catalog of the collection center, and no repeated preservation is required.

[0019] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0020] Preferably, the number of effective live bacteria in the EM bacteria is ≥ 100 billion / g.

[0021] The trace elements are amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated manganese and amino acid chelated iron in a ratio of 1:0.02:0.05:0.5.

[0022] The present invention also provides a method for preparing the above-mentioned high-efficiency liquid organic fertilizer, which comprises the following steps:

[0023] S1: First, the livestock breeding wastewater and the pretreatment agent are mixed in proportion, and then air-dried under natural light for 10-12 hours, and then the solid-liquid separation is performed and the filtrate is collected;

[0024] S2: adding EM bacteria and plant straw to the obtained filtrate, adjusting the temperature of the mixture to 40-45°C, and fermenting for 36-48 hours, followed by solid-liquid separation to obtain a fermentation liquid;

[0025] S3: Thaw and activate Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streak the plates and culture them in a 28°C incubator for 48 hours, take a loop of the cultured strains in LB liquid culture medium, place it in a shaker at 28°C and 200 r / min for 24 hours to obtain seed liquid; inoculate the seed liquids of the four strains into NB liquid culture medium at an inoculation rate of 3%, bottle 200 mL, and culture at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mix the four fermentation liquids in a mass ratio of 1:1:1:1 to obtain a composite microbial agent;

[0026] S4: preparing a sunflower disk extract and combining it with sodium laureth sulfate in a mass ratio of 3:1 to form an accelerator;

[0027] S5: The obtained fermentation liquid, composite microbial agent, promoter, potassium dihydrogen phosphate and trace elements are uniformly mixed according to weight ratio, and then packaged to obtain the final product.

[0028] The high-efficiency liquid organic fertilizer of the present invention pre-treats livestock breeding wastewater through a specific pre-treating agent of the present invention. Since the biochar-TiO2 composite material used has a loose porous structure and a large specific surface area, it can have a good adsorption effect on various heavy metal ions and difficult-to-treat organic pollutants in the wastewater. At the same time, the Mn-doped nano-TiO2 material is also adsorbed in the pores, further increasing the specific surface area and active sites of the composite material, and the adsorption effect is further enhanced. At the same time, the pollutants in the wastewater are enriched on its surface through the adsorption effect, and then TiO2 degrades part of the organic pollutants under light conditions, and can also kill harmful pathogenic bacteria, thereby reducing the content of organic pollutants in the livestock breeding wastewater, purifying the wastewater, and making it more conducive to the growth and reproduction of beneficial microorganisms. EM bacteria and plant straw are added to the livestock breeding wastewater after pre-treatment for fermentation treatment. The fermented product is rich in nutrients such as amino acids, organic acids, polysaccharides, and multiple vitamins, which can provide good nutrients for potatoes, promote potato growth, and improve potato yield and quality.

[0029] The invention also adds a promoter of specific components, which can promote the absorption of nutrients by potatoes on the one hand, and improve the activity of beneficial microorganisms on the other hand; the sunflower disk extract in the promoter is a product obtained by composite enzyme hydrolysis treatment, and the sunflower disk itself includes a variety of active peptides, alkaloids, total flavonoids and polysaccharides and other active substances. After composite enzyme hydrolysis, the macromolecular substances contained therein are decomposed into small molecular components that are easily absorbed, thereby releasing more small molecular active components, which can improve the activity of microorganisms and promote the absorption of nutrients by potatoes; and the combination of sodium laureth sulfate and sunflower disk extract can be more conducive to the absorption and utilization of nutrients by potatoes, and the effect of increasing production and increasing income is more significant.

[0030] The composite microbial agent composed of four strains adopted in the present invention has significant synergistic effect, can increase the content of soil organic matter, improve soil structure, and make the soil more loose and fertile; can secrete active substances, stimulate the growth and development of potato roots, make the root system more developed, and have stronger ability to absorb water and nutrients; can inhibit the reproduction of soil-borne pathogens, reduce the occurrence of plant diseases, and has strong tolerance to the complex environment in the potato rhizosphere environment, good adaptability, and enhances the stress resistance of potatoes.

[0031] The beneficial effects of the present invention are as follows: the present invention realizes the resource utilization of animal husbandry wastewater, converts the wastewater into liquid organic fertilizer rich in nutrient elements, and provides high-quality fertilizer resources for agricultural production; through the combination of biological treatment and physical and chemical treatment technology, harmful substances in the wastewater are effectively removed, ensuring the safety and environmental protection of the organic fertilizer; the liquid organic fertilizer prepared by the present invention can improve the fertility of the soil, promote the growth and development of potatoes, and improve the yield and quality of potatoes, and at the same time can also reduce the use of chemical pesticides, reduce the cost of fertilization, and enhance the environmental adaptability of crops, thereby achieving the purpose of reducing costs and increasing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a test result diagram of dry matter accumulation in different treatment groups of the present invention;

[0033] Figure 2 The plant heights of different treatment groups in the tuber formation period, tuber growth period and starch accumulation period of the present invention;

[0034] Figure 3 It is the main stem diameter of different treatment groups in the tuber formation period, tuber growth period and starch accumulation period of the present invention. DETAILED DESCRIPTION

[0035] The technical solution of the present invention is further described below in conjunction with specific embodiments, but is not limited thereto.

[0036] Example 1

[0037] A highly efficient liquid organic fertilizer is prepared from the following raw materials in parts by weight: 200 kg of livestock breeding wastewater, 50 kg of plant straw, 10 kg of pretreatment agent, 10 kg of EM bacteria, 20 kg of composite microbial agent, 3 kg of promoter, 1 kg of potassium dihydrogen phosphate, and 0.05 kg of trace elements; the promoter is composed of sunflower disk extract and sodium lauryl polyether sulfate in a mass ratio of 3:1; the pretreatment agent is a biochar-TiO2 composite material.

[0038] The biochar-TiO2 composite material is prepared by the following method:

[0039] (1) Take 20 mL of anhydrous ethanol, add 10 mL of tetrabutyl titanate and 1.5 mL of glacial acetic acid in sequence under vigorous stirring, and stir for 30 min to obtain a mixed solution A;

[0040] (2) Take 20 mL of anhydrous ethanol, add 2.5 mL of deionized water to obtain an alcohol-water mixed solution, take 0.45 g of manganese acetate and add it to the above alcohol-water mixed solution, add 0.2 mL of nitric acid dropwise, and stir for 20 min to obtain a mixed solution B;

[0041] (3) Add mixed solution B drop by drop into mixed solution A and continue stirring for 10 min. After aging at room temperature for 6-8 h, a gel is obtained. The gel is placed in an oven, dried at 80 °C, ground into powder, and calcined at 500 °C for 2 h to obtain Mn-doped nano-TiO2 material.

[0042] (4) Take 5 g of rice husk biochar and soak it in 50 ml of 5 mol / L potassium hydroxide solution for 2 h, then take it out and dry it for later use;

[0043] (5) Take 1 g of the Mn-doped nano-TiO2 material obtained in step (3), add it to 100 ml of 2.5 mol / L NaOH solution, and sonicate for 10 min to obtain a nano-TiO2 dispersion;

[0044] (6) Take 5 g of the rice husk biochar treated with alkali in step (4), disperse it in 500 ml of deionized water, then add 20 ml of the nano-TiO2 dispersion obtained in step (5) thereto, ultrasonically treat for 10-20 min, and then react with magnetic stirring for 4 h. The reaction solution is centrifuged, filtered, washed, and dried to obtain a solid product. After vacuum drying at 75°C for 10 h, the solid product is transferred to a tubular furnace and heat treated at 400°C in a nitrogen atmosphere for 2 h to obtain a biochar-TiO2 composite material.

[0045] The preparation method of the sunflower disc extract is as follows: take a sunflower disc, clean it, dry it in an oven at 80°C for 2h, crush it through an 80-mesh sieve to obtain sunflower disc powder, then add a composite enzyme solution for enzymolysis for 8-10h, the enzymolysis temperature is 45-50°C, inactivate it at high temperature, and cool it to room temperature to obtain the sunflower disc extract; the solid-liquid ratio of the sunflower disc powder to the composite enzyme solution is 1g:10ml; the composite enzyme solution contains 10-15g / L papain and 15-20g / L cellulase.

[0046] The composite microbial agent is prepared by the following method: thawing and activating Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis and Trichoderma viride strains respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strains into an LB liquid culture medium, placing the culture medium in a shaking incubator at 28°C and 200 r / min for 24 hours to obtain a seed solution; inoculating the seed solutions of the four strains into NB liquid culture medium at an inoculation rate of 3% respectively, bottling 200 mL, 30°C, 200 r / min shaking culture for 72 hours to obtain a fermentation solution; and mixing the four fermentation solutions in a mass ratio of 1:1:1:1 to obtain a composite microbial agent.

[0047] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0048] The effective live bacteria count in the EM bacteria is ≥100 billion / g.

[0049] The trace elements are amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated manganese and amino acid chelated iron in a ratio of 1:0.02:0.05:0.5.

[0050] The preparation method of the above-mentioned high-efficiency liquid organic fertilizer comprises the following steps:

[0051] S1: First, the livestock breeding wastewater and the pretreatment agent are mixed in proportion, and then air-dried under natural light for 10-12 hours, and then the solid-liquid separation is performed and the filtrate is collected;

[0052] S2: adding EM bacteria and plant straw to the obtained filtrate, adjusting the temperature of the mixture to 40-45°C, and fermenting for 36-48 hours, followed by solid-liquid separation to obtain a fermentation liquid;

[0053] S3: Thaw and activate Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streak the plates and culture them in a 28°C incubator for 48 hours, take a loop of the cultured strains in LB liquid culture medium, place it in a shaker at 28°C and 200 r / min for 24 hours to obtain seed liquid; inoculate the seed liquids of the four strains into NB liquid culture medium at an inoculation rate of 3%, bottle 200 mL, and culture at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mix the four fermentation liquids in a mass ratio of 1:1:1:1 to obtain a composite microbial agent;

[0054] S4: preparing a sunflower disk extract and combining it with sodium laureth sulfate in a mass ratio of 3:1 to form an accelerator;

[0055] S5: The obtained fermentation liquid, composite microbial agent, promoter, potassium dihydrogen phosphate and trace elements are uniformly mixed according to weight ratio, and then packaged to obtain the final product.

[0056] Example 2

[0057] A highly efficient liquid organic fertilizer is made from the following raw materials in parts by weight: 300 kg of livestock breeding wastewater, 80 kg of plant straw, 20 kg of pretreatment agent, 20 kg of EM bacteria, 30 kg of composite microbial agent, 10 kg of promoter, 3 kg of potassium dihydrogen phosphate, and 0.2 kg of trace elements; the promoter is composed of sunflower disc extract and sodium lauryl polyether sulfate in a mass ratio of 3:1; the pretreatment agent is a biochar-TiO2 composite material.

[0058] The biochar-TiO2 composite material is prepared by the following method:

[0059] (1) Take 20 mL of anhydrous ethanol, add 10 mL of tetrabutyl titanate and 1.5 mL of glacial acetic acid in sequence under vigorous stirring, and stir for 30 min to obtain a mixed solution A;

[0060] (2) Take 20 mL of anhydrous ethanol, add 2.5 mL of deionized water to obtain an alcohol-water mixed solution, take 0.45 g of manganese acetate and add it to the above alcohol-water mixed solution, add 0.2 mL of nitric acid dropwise, and stir for 20 min to obtain a mixed solution B;

[0061] (3) Add mixed solution B drop by drop into mixed solution A and continue stirring for 10 min. After aging at room temperature for 6-8 h, a gel is obtained. The gel is placed in an oven, dried at 80 °C, ground into powder, and calcined at 500 °C for 2 h to obtain Mn-doped nano-TiO2 material.

[0062] (4) Take 5 g of rice husk biochar and soak it in 50 ml of 5 mol / L potassium hydroxide solution for 2 h, then take it out and dry it for later use;

[0063] (5) Take 1 g of the Mn-doped nano-TiO2 material obtained in step (3), add it to 100 ml of 2.5 mol / L NaOH solution, and sonicate for 10 min to obtain a nano-TiO2 dispersion;

[0064] (6) Take 5 g of the rice husk biochar treated with alkali in step (4), disperse it in 500 ml of deionized water, then add 20 ml of the nano-TiO2 dispersion obtained in step (5) thereto, ultrasonically treat for 10-20 min, and then react with magnetic stirring for 4 h. The reaction solution is centrifuged, filtered, washed, and dried to obtain a solid product. After vacuum drying at 75°C for 10 h, the solid product is transferred to a tubular furnace and heat treated at 400°C in a nitrogen atmosphere for 2 h to obtain a biochar-TiO2 composite material.

[0065] The preparation method of the sunflower disc extract is as follows: take a sunflower disc, clean it, dry it in an oven at 80°C for 2h, crush it through an 80-mesh sieve to obtain sunflower disc powder, then add a composite enzyme solution for enzymolysis for 8-10h, the enzymolysis temperature is 45-50°C, inactivate it at high temperature, and cool it to room temperature to obtain the sunflower disc extract; the solid-liquid ratio of the sunflower disc powder to the composite enzyme solution is 1g:10ml; the composite enzyme solution contains 10-15g / L papain and 15-20g / L cellulase.

[0066] The composite microbial agent is prepared by the following method: thawing and activating Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis and Trichoderma viride strains respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strains into an LB liquid culture medium, placing the culture medium in a shaking incubator at 28°C and 200 r / min for 24 hours to obtain a seed solution; inoculating the seed solutions of the four strains into NB liquid culture medium at an inoculation rate of 3% respectively, bottling 200 mL, 30°C, 200 r / min shaking culture for 72 hours to obtain a fermentation solution; and mixing the four fermentation solutions in a mass ratio of 1:1:1:1 to obtain a composite microbial agent.

[0067] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0068] The effective live bacteria count in the EM bacteria is ≥100 billion / g.

[0069] The trace elements are amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated manganese and amino acid chelated iron in a ratio of 1:0.02:0.05:0.5.

[0070] The preparation method of the above-mentioned high-efficiency liquid organic fertilizer comprises the following steps:

[0071] S1: First, the livestock breeding wastewater and the pretreatment agent are mixed in proportion, and then air-dried under natural light for 10-12 hours, and then the solid-liquid separation is performed and the filtrate is collected;

[0072] S2: adding EM bacteria and plant straw to the obtained filtrate, adjusting the temperature of the mixture to 40-45°C, and fermenting for 36-48 hours, followed by solid-liquid separation to obtain a fermentation liquid;

[0073] S3: Thaw and activate Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streak the plates and culture them in a 28°C incubator for 48 hours, take a loop of the cultured strains in LB liquid culture medium, place it in a shaker at 28°C and 200 r / min for 24 hours to obtain seed liquid; inoculate the seed liquids of the four strains into NB liquid culture medium at an inoculation rate of 3%, bottle 200 mL, and culture at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mix the four fermentation liquids in a mass ratio of 1:1:1:1 to obtain a composite microbial agent;

[0074] S4: preparing a sunflower disk extract and combining it with sodium laureth sulfate in a mass ratio of 3:1 to form an accelerator;

[0075] S5: The obtained fermentation liquid, composite microbial agent, promoter, potassium dihydrogen phosphate and trace elements are uniformly mixed according to weight ratio, and then packaged to obtain the final product.

[0076] Example 3

[0077] A highly efficient liquid organic fertilizer is made from the following raw materials in parts by weight: 260kg of livestock breeding wastewater, 70kg of plant straw, 15kg of pretreatment agent, 15kg of EM bacteria, 25kg of composite microbial agent, 6kg of promoter, 2kg of potassium dihydrogen phosphate, and 0.1kg of trace elements; the promoter is composed of sunflower disc extract and sodium lauryl polyether sulfate in a mass ratio of 3:1; the pretreatment agent is a biochar-TiO2 composite material.

[0078] The biochar-TiO2 composite material is prepared by the following method:

[0079] (1) Take 20 mL of anhydrous ethanol, add 10 mL of tetrabutyl titanate and 1.5 mL of glacial acetic acid in sequence under vigorous stirring, and stir for 30 min to obtain a mixed solution A;

[0080] (2) Take 20 mL of anhydrous ethanol, add 2.5 mL of deionized water to obtain an alcohol-water mixed solution, take 0.45 g of manganese acetate and add it to the above alcohol-water mixed solution, add 0.2 mL of nitric acid dropwise, and stir for 20 min to obtain a mixed solution B;

[0081] (3) Add mixed solution B drop by drop into mixed solution A and continue stirring for 10 min. After aging at room temperature for 6-8 h, a gel is obtained. The gel is placed in an oven, dried at 80 °C, ground into powder, and calcined at 500 °C for 2 h to obtain Mn-doped nano-TiO2 material.

[0082] (4) Take 5 g of rice husk biochar and soak it in 50 ml of 5 mol / L potassium hydroxide solution for 2 h, then take it out and dry it for later use;

[0083] (5) Take 1 g of the Mn-doped nano-TiO2 material obtained in step (3), add it to 100 ml of 2.5 mol / L NaOH solution, and sonicate for 10 min to obtain a nano-TiO2 dispersion;

[0084] (6) Take 5 g of the rice husk biochar treated with alkali in step (4), disperse it in 500 ml of deionized water, then add 20 ml of the nano-TiO2 dispersion obtained in step (5) thereto, ultrasonically treat for 10-20 min, and then react with magnetic stirring for 4 h. The reaction solution is centrifuged, filtered, washed, and dried to obtain a solid product. After vacuum drying at 75°C for 10 h, the solid product is transferred to a tubular furnace and heat treated at 400°C in a nitrogen atmosphere for 2 h to obtain a biochar-TiO2 composite material.

[0085] The preparation method of the sunflower disc extract is as follows: take a sunflower disc, clean it, dry it in an oven at 80°C for 2h, crush it through an 80-mesh sieve to obtain sunflower disc powder, then add a composite enzyme solution for enzymolysis for 8-10h, the enzymolysis temperature is 45-50°C, inactivate it at high temperature, and cool it to room temperature to obtain the sunflower disc extract; the solid-liquid ratio of the sunflower disc powder to the composite enzyme solution is 1g:10ml; the composite enzyme solution contains 10-15g / L papain and 15-20g / L cellulase.

[0086] The composite microbial agent is prepared by the following method: thawing and activating Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis and Trichoderma viride strains respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strains into an LB liquid culture medium, placing the culture medium in a shaking incubator at 28°C and 200 r / min for 24 hours to obtain a seed solution; inoculating the seed solutions of the four strains into NB liquid culture medium at an inoculation rate of 3% respectively, bottling 200 mL, 30°C, 200 r / min shaking culture for 72 hours to obtain a fermentation solution; and mixing the four fermentation solutions in a mass ratio of 1:1:1:1 to obtain a composite microbial agent.

[0087] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0088] The effective live bacteria count in the EM bacteria is ≥100 billion / g.

[0089] The trace elements are amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated manganese and amino acid chelated iron in a ratio of 1:0.02:0.05:0.5.

[0090] The preparation method of the above-mentioned high-efficiency liquid organic fertilizer comprises the following steps:

[0091] S1: First, the livestock breeding wastewater and the pretreatment agent are mixed in proportion, and then air-dried under natural light for 10-12 hours, and then the solid-liquid separation is performed and the filtrate is collected;

[0092] S2: adding EM bacteria and plant straw to the obtained filtrate, adjusting the temperature of the mixture to 40-45°C, and fermenting for 36-48 hours, followed by solid-liquid separation to obtain a fermentation liquid;

[0093] S3: Thaw and activate Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streak the plates and culture them in a 28°C incubator for 48 hours, take a loop of the cultured strains in LB liquid culture medium, place it in a shaker at 28°C and 200 r / min for 24 hours to obtain seed liquid; inoculate the seed liquids of the four strains into NB liquid culture medium at an inoculation rate of 3%, bottle 200 mL, and culture at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mix the four fermentation liquids in a mass ratio of 1:1:1:1 to obtain a composite microbial agent;

[0094] S4: preparing a sunflower disk extract and combining it with sodium laureth sulfate in a mass ratio of 3:1 to form an accelerator;

[0095] S5: The obtained fermentation liquid, composite microbial agent, promoter, potassium dihydrogen phosphate and trace elements are uniformly mixed according to weight ratio, and then packaged to obtain the final product.

[0096] Comparative Example 1

[0097] A highly efficient liquid organic fertilizer is prepared from the following raw materials in parts by weight: 260 kg of livestock breeding wastewater, 70 kg of plant straw, 15 kg of EM bacteria, 25 kg of composite microbial agent, 6 kg of promoter, 2 kg of potassium dihydrogen phosphate, and 0.1 kg of trace elements; the promoter is composed of sunflower disk extract and sodium lauryl polyether sulfate in a mass ratio of 3:1.

[0098] The preparation method of the sunflower disc extract is as follows: take a sunflower disc, clean it, dry it in an oven at 80°C for 2h, crush it through an 80-mesh sieve to obtain sunflower disc powder, then add a composite enzyme solution for enzymolysis for 8-10h, the enzymolysis temperature is 45-50°C, inactivate it at high temperature, and cool it to room temperature to obtain the sunflower disc extract; the solid-liquid ratio of the sunflower disc powder to the composite enzyme solution is 1g:10ml; the composite enzyme solution contains 10-15g / L papain and 15-20g / L cellulase.

[0099] The composite microbial agent is prepared by the following method: thawing and activating Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis and Trichoderma viride strains respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strains into an LB liquid culture medium, placing the culture medium in a shaking incubator at 28°C and 200 r / min for 24 hours to obtain a seed solution; inoculating the seed solutions of the four strains into NB liquid culture medium at an inoculation rate of 3% respectively, bottling 200 mL, 30°C, 200 r / min shaking culture for 72 hours to obtain a fermentation solution; and mixing the four fermentation solutions in a mass ratio of 1:1:1:1 to obtain a composite microbial agent.

[0100] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0101] The effective live bacteria count in the EM bacteria is ≥100 billion / g.

[0102] The trace elements are amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated manganese and amino acid chelated iron in a ratio of 1:0.02:0.05:0.5.

[0103] The preparation method of the above-mentioned high-efficiency liquid organic fertilizer comprises the following steps:

[0104] S1: First, the livestock breeding wastewater is dried under natural light for 10-12 hours, then the solid-liquid separation is performed and the filtrate is collected;

[0105] S2: adding EM bacteria and plant straw to the obtained filtrate, adjusting the temperature of the mixture to 40-45°C, and fermenting for 36-48 hours, followed by solid-liquid separation to obtain a fermentation liquid;

[0106] S3: Thaw and activate Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streak the plates and culture them in a 28°C incubator for 48 hours, take a loop of the cultured strains in LB liquid culture medium, place it in a shaker at 28°C and 200 r / min for 24 hours to obtain seed liquid; inoculate the seed liquids of the four strains into NB liquid culture medium at an inoculation rate of 3%, bottle 200 mL, and culture at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mix the four fermentation liquids in a mass ratio of 1:1:1:1 to obtain a composite microbial agent;

[0107] S4: preparing a sunflower disk extract and combining it with sodium laureth sulfate in a mass ratio of 3:1 to form an accelerator;

[0108] S5: The obtained fermentation liquid, composite microbial agent, promoter, potassium dihydrogen phosphate and trace elements are uniformly mixed according to weight ratio, and then packaged to obtain the final product.

[0109] This comparative example is basically the same as Example 3, the only difference being that it does not contain a pretreatment agent and the step of pretreating the livestock breeding wastewater with the pretreatment agent.

[0110] Comparative Example 2

[0111] A highly efficient liquid organic fertilizer is made from the following raw materials in parts by weight: 260 kg of livestock breeding wastewater, 70 kg of plant straw, 15 kg of pretreatment agent, 15 kg of EM bacteria, 25 kg of composite microbial agent, 2 kg of potassium dihydrogen phosphate, and 0.1 kg of trace elements; the pretreatment agent is a biochar-TiO2 composite material.

[0112] The biochar-TiO2 composite material is prepared by the following method:

[0113] (1) Take 20 mL of anhydrous ethanol, add 10 mL of tetrabutyl titanate and 1.5 mL of glacial acetic acid in sequence under vigorous stirring, and stir for 30 min to obtain a mixed solution A;

[0114] (2) Take 20 mL of anhydrous ethanol, add 2.5 mL of deionized water to obtain an alcohol-water mixed solution, take 0.45 g of manganese acetate and add it to the above alcohol-water mixed solution, add 0.2 mL of nitric acid dropwise, and stir for 20 min to obtain a mixed solution B;

[0115] (3) Add mixed solution B drop by drop into mixed solution A and continue stirring for 10 min. After aging at room temperature for 6-8 h, a gel is obtained. The gel is placed in an oven, dried at 80 °C, ground into powder, and calcined at 500 °C for 2 h to obtain Mn-doped nano-TiO2 material.

[0116] (4) Take 5 g of rice husk biochar and soak it in 50 ml of 5 mol / L potassium hydroxide solution for 2 h, then take it out and dry it for later use;

[0117] (5) Take 1 g of the Mn-doped nano-TiO2 material obtained in step (3), add it to 100 ml of 2.5 mol / L NaOH solution, and sonicate for 10 min to obtain a nano-TiO2 dispersion;

[0118] (6) Take 5 g of the rice husk biochar treated with alkali in step (4), disperse it in 500 ml of deionized water, then add 20 ml of the nano-TiO2 dispersion obtained in step (5) thereto, ultrasonically treat for 10-20 min, and then react with magnetic stirring for 4 h. The reaction solution is centrifuged, filtered, washed, and dried to obtain a solid product. After vacuum drying at 75°C for 10 h, the solid product is transferred to a tubular furnace and heat treated at 400°C in a nitrogen atmosphere for 2 h to obtain a biochar-TiO2 composite material.

[0119] The composite microbial agent is prepared by the following method: thawing and activating Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis and Trichoderma viride strains respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strains into an LB liquid culture medium, placing the culture medium in a shaking incubator at 28°C and 200 r / min for 24 hours to obtain a seed solution; inoculating the seed solutions of the four strains into NB liquid culture medium at an inoculation rate of 3% respectively, bottling 200 mL, 30°C, 200 r / min shaking culture for 72 hours to obtain a fermentation solution; and mixing the four fermentation solutions in a mass ratio of 1:1:1:1 to obtain a composite microbial agent.

[0120] The Bacillus licheniformis strain is numbered as CGMCC No.1.10257, purchased from China General Microorganism Culture Collection Administration Center, and the preservation date is November 4, 2009; the Bacillus subtilis strain is numbered as CGMCC No.1.7740, purchased from China General Microorganism Culture Collection Administration Center, and the preservation date is July 24, 2008; the Pseudomonas chlororaphis strain is numbered as CGMCC No.1.2785, and the preservation date is July 17, 2001; the Trichoderma viride strain is numbered as CGMCC No.3.7338, and the preservation date is July 8, 2005. The Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride used in the present invention can be purchased through the strain catalog of the collection center, and no repeated preservation is required.

[0121] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0122] The effective live bacteria count in the EM bacteria is ≥100 billion / g.

[0123] The trace elements are amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated manganese and amino acid chelated iron in a ratio of 1:0.02:0.05:0.5.

[0124] The preparation method of the above-mentioned high-efficiency liquid organic fertilizer comprises the following steps:

[0125] S1: First, the livestock breeding wastewater and the pretreatment agent are mixed in proportion, and then air-dried under natural light for 10-12 hours, and then the solid-liquid separation is performed and the filtrate is collected;

[0126] S2: adding EM bacteria and plant straw to the obtained filtrate, adjusting the temperature of the mixture to 40-45°C, and fermenting for 36-48 hours, followed by solid-liquid separation to obtain a fermentation liquid;

[0127] S3: Thaw and activate Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streak the plates and culture them in a 28°C incubator for 48 hours, take a loop of the cultured strains in LB liquid culture medium, place it in a shaker at 28°C and 200 r / min for 24 hours to obtain seed liquid; inoculate the seed liquids of the four strains into NB liquid culture medium at an inoculation rate of 3%, bottle 200 mL, and culture at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mix the four fermentation liquids in a mass ratio of 1:1:1:1 to obtain a composite microbial agent;

[0128] S4: The obtained fermentation liquid, composite microbial agent, potassium dihydrogen phosphate and trace elements are uniformly mixed according to weight proportions and packaged to obtain the final product.

[0129] This comparative example is basically the same as Example 3, except that it does not contain an accelerator component.

[0130] Comparative Example 3

[0131] A highly efficient liquid organic fertilizer is made from the following raw materials in parts by weight: 260 kg of livestock breeding wastewater, 70 kg of plant straw, 15 kg of pretreatment agent, 15 kg of EM bacteria, 25 kg of composite microbial agent, 6 kg of promoter, 2 kg of potassium dihydrogen phosphate, and 0.1 kg of trace elements; the promoter is a sunflower disc extract; and the pretreatment agent is a biochar-TiO2 composite material.

[0132] The biochar-TiO2 composite material is prepared by the following method:

[0133] (1) Take 20 mL of anhydrous ethanol, add 10 mL of tetrabutyl titanate and 1.5 mL of glacial acetic acid in sequence under vigorous stirring, and stir for 30 min to obtain a mixed solution A;

[0134] (2) Take 20 mL of anhydrous ethanol, add 2.5 mL of deionized water to obtain an alcohol-water mixed solution, take 0.45 g of manganese acetate and add it to the above alcohol-water mixed solution, add 0.2 mL of nitric acid dropwise, and stir for 20 min to obtain a mixed solution B;

[0135] (3) Add mixed solution B drop by drop into mixed solution A and continue stirring for 10 min. After aging at room temperature for 6-8 h, a gel is obtained. The gel is placed in an oven, dried at 80 °C, ground into powder, and calcined at 500 °C for 2 h to obtain Mn-doped nano-TiO2 material.

[0136] (4) Take 5 g of rice husk biochar and soak it in 50 ml of 5 mol / L potassium hydroxide solution for 2 h, then take it out and dry it for later use;

[0137] (5) Take 1 g of the Mn-doped nano-TiO2 material obtained in step (3), add it to 100 ml of 2.5 mol / L NaOH solution, and sonicate for 10 min to obtain a nano-TiO2 dispersion;

[0138] (6) Take 5 g of the rice husk biochar treated with alkali in step (4), disperse it in 500 ml of deionized water, then add 20 ml of the nano-TiO2 dispersion obtained in step (5) thereto, ultrasonically treat for 10-20 min, and then react with magnetic stirring for 4 h. The reaction solution is centrifuged, filtered, washed, and dried to obtain a solid product. After vacuum drying at 75°C for 10 h, the solid product is transferred to a tubular furnace and heat treated at 400°C in a nitrogen atmosphere for 2 h to obtain a biochar-TiO2 composite material.

[0139] The preparation method of the sunflower disc extract is as follows: take a sunflower disc, clean it, dry it in an oven at 80°C for 2h, crush it through an 80-mesh sieve to obtain sunflower disc powder, then add a composite enzyme solution for enzymolysis for 8-10h, the enzymolysis temperature is 45-50°C, inactivate it at high temperature, and cool it to room temperature to obtain the sunflower disc extract; the solid-liquid ratio of the sunflower disc powder to the composite enzyme solution is 1g:10ml; the composite enzyme solution contains 10-15g / L papain and 15-20g / L cellulase.

[0140] The composite microbial agent is prepared by the following method: thawing and activating Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis and Trichoderma viride strains respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strains into an LB liquid culture medium, placing the culture medium in a shaking incubator at 28°C and 200 r / min for 24 hours to obtain a seed solution; inoculating the seed solutions of the four strains into NB liquid culture medium at an inoculation rate of 3% respectively, bottling 200 mL, 30°C, 200 r / min shaking culture for 72 hours to obtain a fermentation solution; and mixing the four fermentation solutions in a mass ratio of 1:1:1:1 to obtain a composite microbial agent.

[0141] The Bacillus licheniformis strain is numbered as CGMCC No.1.10257, purchased from China General Microorganism Culture Collection Administration Center, and the preservation date is November 4, 2009; the Bacillus subtilis strain is numbered as CGMCC No.1.7740, purchased from China General Microorganism Culture Collection Administration Center, and the preservation date is July 24, 2008; the Pseudomonas chlororaphis strain is numbered as CGMCC No.1.2785, and the preservation date is July 17, 2001; the Trichoderma viride strain is numbered as CGMCC No.3.7338, and the preservation date is July 8, 2005. The Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride used in the present invention can be purchased through the strain catalog of the collection center, and no repeated preservation is required.

[0142] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0143] The effective live bacteria count in the EM bacteria is ≥100 billion / g.

[0144] The trace elements are amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated manganese and amino acid chelated iron in a ratio of 1:0.02:0.05:0.5.

[0145] The preparation method of the above-mentioned high-efficiency liquid organic fertilizer comprises the following steps:

[0146] S1: First, the livestock breeding wastewater and the pretreatment agent are mixed in proportion, and then air-dried under natural light for 10-12 hours, and then the solid-liquid separation is performed and the filtrate is collected;

[0147] S2: adding EM bacteria and plant straw to the obtained filtrate, adjusting the temperature of the mixture to 40-45°C, and fermenting for 36-48 hours, followed by solid-liquid separation to obtain a fermentation liquid;

[0148] S3: Thaw and activate Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streak the plates and culture them in a 28°C incubator for 48 hours, take a loop of the cultured strains in LB liquid culture medium, place it in a shaker at 28°C and 200 r / min for 24 hours to obtain seed liquid; inoculate the seed liquids of the four strains into NB liquid culture medium at an inoculation rate of 3%, bottle 200 mL, and culture at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mix the four fermentation liquids in a mass ratio of 1:1:1:1 to obtain a composite microbial agent;

[0149] S4: preparing the accelerator sunflower disc extract;

[0150] S5: The obtained fermentation liquid, composite microbial agent, promoter, potassium dihydrogen phosphate and trace elements are uniformly mixed according to weight ratio, and then packaged to obtain the final product.

[0151] This comparative example is basically the same as Example 3, the only difference being that the promoter is only sunflower disk extract.

[0152] Comparative Example 4

[0153] A highly efficient liquid organic fertilizer is made from the following raw materials in parts by weight: 260 kg of livestock breeding wastewater, 70 kg of plant straw, 15 kg of pretreatment agent, 15 kg of EM bacteria, 25 kg of composite microbial agent, 6 kg of promoter, 2 kg of potassium dihydrogen phosphate, and 0.1 kg of trace elements; the promoter is sodium lauryl polyether sulfate; and the pretreatment agent is a biochar-TiO2 composite material.

[0154] The biochar-TiO2 composite material is prepared by the following method:

[0155] (1) Take 20 mL of anhydrous ethanol, add 10 mL of tetrabutyl titanate and 1.5 mL of glacial acetic acid in sequence under vigorous stirring, and stir for 30 min to obtain a mixed solution A;

[0156] (2) Take 20 mL of anhydrous ethanol, add 2.5 mL of deionized water to obtain an alcohol-water mixed solution, take 0.45 g of manganese acetate and add it to the above alcohol-water mixed solution, add 0.2 mL of nitric acid dropwise, and stir for 20 min to obtain a mixed solution B;

[0157] (3) Add mixed solution B drop by drop into mixed solution A and continue stirring for 10 min. After aging at room temperature for 6-8 h, a gel is obtained. The gel is placed in an oven, dried at 80 °C, ground into powder, and calcined at 500 °C for 2 h to obtain Mn-doped nano-TiO2 material.

[0158] (4) Take 5 g of rice husk biochar and soak it in 50 ml of 5 mol / L potassium hydroxide solution for 2 h, then take it out and dry it for later use;

[0159] (5) Take 1 g of the Mn-doped nano-TiO2 material obtained in step (3), add it to 100 ml of 2.5 mol / L NaOH solution, and sonicate for 10 min to obtain a nano-TiO2 dispersion;

[0160] (6) Take 5 g of the rice husk biochar treated with alkali in step (4), disperse it in 500 ml of deionized water, then add 20 ml of the nano-TiO2 dispersion obtained in step (5) thereto, ultrasonically treat for 10-20 min, and then react with magnetic stirring for 4 h. The reaction solution is centrifuged, filtered, washed, and dried to obtain a solid product. After vacuum drying at 75°C for 10 h, the solid product is transferred to a tubular furnace and heat treated at 400°C in a nitrogen atmosphere for 2 h to obtain a biochar-TiO2 composite material.

[0161] The composite microbial agent is prepared by the following method: thawing and activating Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis and Trichoderma viride strains respectively, culturing in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strains into an LB liquid culture medium, placing the culture medium in a shaking incubator at 28°C and 200 r / min for 24 hours to obtain a seed solution; inoculating the seed solutions of the four strains into NB liquid culture medium at an inoculation rate of 3% respectively, bottling 200 mL, 30°C, 200 r / min shaking culture for 72 hours to obtain a fermentation solution; and mixing the four fermentation solutions in a mass ratio of 1:1:1:1 to obtain a composite microbial agent.

[0162] The LB liquid culture medium is composed of: 5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 1000 mL distilled water, pH adjusted to 7.0; and sterilized at 121° C. for 20 min.

[0163] The effective live bacteria count in the EM bacteria is ≥100 billion / g.

[0164] The trace elements are amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated manganese and amino acid chelated iron in a ratio of 1:0.02:0.05:0.5.

[0165] The preparation method of the above-mentioned high-efficiency liquid organic fertilizer comprises the following steps:

[0166] S1: First, the livestock breeding wastewater and the pretreatment agent are mixed in proportion, and then air-dried under natural light for 10-12 hours, and then the solid-liquid separation is performed and the filtrate is collected;

[0167] S2: adding EM bacteria and plant straw to the obtained filtrate, adjusting the temperature of the mixture to 40-45°C, and fermenting for 36-48 hours, followed by solid-liquid separation to obtain a fermentation liquid;

[0168] S3: Thaw and activate Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streak the plates and culture them in a 28°C incubator for 48 hours, take a loop of the cultured strains in LB liquid culture medium, place it in a shaker at 28°C and 200 r / min for 24 hours to obtain seed liquid; inoculate the seed liquids of the four strains into NB liquid culture medium at an inoculation rate of 3%, bottle 200 mL, and culture at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; mix the four fermentation liquids in a mass ratio of 1:1:1:1 to obtain a composite microbial agent;

[0169] S4: The obtained fermentation liquid, composite microbial agent, sodium lauryl polyether sulfate, potassium dihydrogen phosphate and trace elements are uniformly mixed according to weight proportions, and then packaged to obtain the final product.

[0170] This comparative example is basically the same as Example 3, the only difference being that the accelerator is only sodium lauryl polyether sulfate.

[0171] Fertilizer efficiency verification test

[0172] The high-efficiency liquid organic fertilizer prepared in Examples 1-3 and Comparative Examples 1-4 was used for field potato planting test. A potato planting base in Qitai Town, Shangdu County, Ulanbucha City, Inner Mongolia Autonomous Region was selected for the test. The potato variety was Huasong No. 7. A total of 8 treatments were set up in the test, namely, the treatment groups of Examples 1-3, Comparative Examples 1-4 and the control treatment group of commercially available water-soluble fertilizer (N:P:K=13:6:40), and the area of ​​each plot was 1 mu. The large ridges were planted in 2 rows, the ridge width was 40 cm, the ridge height was 25 cm, and the planting density was 55,000 plants / hm 2 The application rate of each liquid organic fertilizer was 5kg / mu, and the fertilizer was applied with water after potato planting. The control group was applied with commercially available ordinary water-soluble fertilizer (N:P:K=13:6:40), and other conventional field management was the same. The basic physical and chemical properties of the experimental field soil are shown in Table 1:

[0173] Table 1 Physical and chemical properties of soil

[0174]

[0175] The yield was measured when potatoes were harvested. The crude protein, reducing sugar, Vc content, starch content, and nitrate content were measured within 14 days after harvest. The specific test results are shown in Table 2.

[0176] Table 2 Effects of different treatments on potato yield and quality

[0177]

[0178] As can be seen from the contents of Table 2 above, the high-efficiency liquid organic fertilizer prepared by Examples 1-3 of the present invention has a significant effect on increasing potato production, with an increase of more than 11.1% compared with the commercial control group of conventional fertilizers; the crude protein content, reducing sugar content, Vc content and starch content of potatoes after using the liquid organic fertilizer of the present invention are significantly improved, the nitrate content is significantly reduced, and the quality of potatoes is effectively improved. This is because the pretreatment agent prepared by the present invention can have good adsorption and degradation effects on various heavy metal ions and difficult-to-treat organic pollutants in wastewater, and can also kill various pathogenic bacteria, so that the pretreated livestock breeding wastewater no longer has the traditional "three high" characteristics, which is more conducive to the reproduction and growth of microorganisms. The promoter contains small molecule active ingredients that are easier for microorganisms and plants to absorb, which can improve the activity of microorganisms and promote the absorption of nutrients by potatoes; the two complement each other and are indispensable.

[0179] From the seedling stage to the mature stage of potato, three plants with uniform growth were randomly selected from Example 3, Comparative Examples 1-4 and the commercially available control treatment group, and the samples were bagged after measuring the fresh weight. They were taken back to the laboratory and dried at 105°C for 30 min, and then the temperature was adjusted to 80°C and dried to constant weight for measuring dry matter. The results of dry matter accumulation in different treatment groups are shown in Figure 2. Figure 1 As shown. Figure 1 As can be seen from the data in, the potato dry matter accumulation generally shows a variation pattern of slow growth-rapid growth-slow growth, wherein the growth rate is the fastest during the potato tuber expansion period and starch accumulation period, and reaches a peak value during the maturity period. In the entire growth period of potatoes, the dry matter accumulation growth rate of Example 3 is the fastest, and is significantly better than other treatment groups, which is consistent with the potato yield structure composition. This is because the synergistic effect of each component of the high-efficiency liquid organic fertilizer of the present invention can promote the absorption and utilization of nutrients by potatoes, thereby improving yield and quality.

[0180] Randomly select 3 plants with the same growth from Example 3, Comparative Examples 1-4 and the commercially available control treatment group, measure the plant height and main stem diameter during the tuber formation period, tuber growth period and starch accumulation period, and take the average value. Figure 2 , Figure 3 As shown. Figure 2 and Figure 3 It can be seen that after using the high-efficiency liquid organic fertilizer prepared by the present invention, the average plant height and main stem thickness of potatoes are significantly higher than those of comparative examples 1-4 and the commercially available treatment control group, which indicates that the high-efficiency liquid organic fertilizer of the present invention has a significant effect on promoting potato growth and has good application prospects in potato planting.

[0181] After the potato harvest, the surface soil (0-30 cm) samples were collected. The soil samples were collected from each treatment group according to the S-shaped sampling method. When collecting soil, the soil drill was drilled to a depth of 30 cm at one time. The samples were collected three times for each treatment group, and the three replicates of the same treatment group were mixed into one sample. The visible debris such as gravel and plant roots were removed and brought back to the laboratory for determination of basic physical and chemical properties of the soil. The results are shown in Table 3 below.

[0182] Table 3 Soil test results of different treatment groups after potato planting

[0183]

[0184] It should be noted that the above embodiments are only partial embodiments of the preferred methods of implementing the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A high-efficiency liquid organic fertilizer, characterized in that: It is made of the following raw materials in parts by weight: 200-300 parts of livestock breeding wastewater, 50-80 parts of plant straw, 10-20 parts of pretreatment agent, 10-20 parts of EM bacteria, 20-30 parts of composite microbial agent, 3-10 parts of promoter, 1-3 parts of potassium dihydrogen phosphate, and 0.05-0.2 parts of trace elements; the promoter is composed of sunflower disk extract and sodium lauryl polyether sulfate in a mass ratio of 3:1; the pretreatment agent is a biochar-TiO2 composite material; The biochar-TiO2 composite material is prepared by the following method: (1) Take 20 mL of anhydrous ethanol, add 10 mL of tetrabutyl titanate and 1.5 mL of glacial acetic acid in sequence under vigorous stirring, and stir for 30 minutes to obtain a mixed solution A; (2) Take 20 mL of anhydrous ethanol, add 2.5 mL of deionized water to obtain an alcohol-water mixed solution, take 0.45 g of manganese acetate and add it to the above alcohol-water mixed solution, add 0.2 mL of nitric acid dropwise, and stir for 20 min to obtain a mixed solution B; (3) Add mixed solution B drop by drop into mixed solution A and continue stirring for 10 min. After aging at room temperature for 6-8 h, a gel is obtained. The gel is placed in an oven, dried at 80 °C, ground into powder, and calcined at 500 °C for 2 h to obtain Mn-doped nano-TiO2 material. (4) Take 5 g of rice husk biochar and soak it in 50 ml of 5 mol / L potassium hydroxide solution for 2 h, then take it out and dry it for later use; (5) Take 1 g of the Mn-doped nano-TiO2 material obtained in step (3), add it to 100 ml of 2.5 mol / L NaOH solution, and sonicate for 10 min to obtain a nano-TiO2 dispersion; (6) 5 g of the rice husk biochar treated with alkali in step (4) was dispersed in 500 ml of deionized water, and then 20 ml of the nano-TiO2 dispersion obtained in step (5) was added thereto. The mixture was ultrasonically treated for 10-20 min, and then subjected to magnetic stirring for 4 h. The reaction solution was centrifuged, filtered, washed, and dried to obtain a solid product. The solid product was vacuum dried at 75° C. for 10 h, and then transferred to a tubular furnace and heat treated at 400° C. in a nitrogen atmosphere for 2 h to obtain a biochar-TiO2 composite material. The preparation method of the sunflower disc extract is as follows: take a sunflower disc, clean it, dry it in an oven at 80°C for 2h, crush it through an 80-mesh sieve to obtain sunflower disc powder, then add a composite enzyme solution for enzymolysis for 8-10h, the enzymolysis temperature is 45-50°C, inactivate it at high temperature, and cool it to room temperature to obtain the sunflower disc extract.

2. High-efficiency liquid organic fertilizer according to claim 1, is characterized in that, The material-liquid ratio of the sunflower disk powder to the complex enzyme solution is 1g:10ml.

3. High-efficiency liquid organic fertilizer according to claim 1, is characterized in that, The complex enzyme solution contains 10-15 g / L of papain and 15-20 g / L of cellulase.

4. High-efficiency liquid organic fertilizer according to claim 1, is characterized in that, The composite microbial agent is prepared by the following method: thawing and activating Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis and Trichoderma viride strains respectively, culturing them in a 28°C incubator for 48 hours after streaking on a plate, taking a loop of the cultured strains in LB liquid culture medium, placing it in a shaking incubator at 28°C and 200 r / min for 24 hours to obtain a seed solution; The seed liquid of the four strains was inoculated into NB liquid culture medium at an inoculation rate of 3%, the bottling volume was 200 mL, and the culture was shaken at 30°C and 200 r / min for 72 h to obtain fermentation liquid; the four fermentation liquids were mixed in a mass ratio of 1:1:1:1 to obtain a composite microbial agent.

5. High-efficiency liquid organic fertilizer according to claim 1, characterized in that, The effective live bacteria count in the EM bacteria is ≥100 billion / g.

6. High-efficiency liquid organic fertilizer according to claim 1, characterized in that, The trace elements are amino acid chelated zinc, amino acid chelated magnesium, amino acid chelated manganese and amino acid chelated iron in a ratio of 1:0.02:0.05:0.

5.

7. A method for preparing a high-efficiency liquid organic fertilizer according to any one of claims 1 to 6, characterized in that: It includes the following steps: S1: First, the livestock breeding wastewater and the pretreatment agent are mixed in proportion, and then air-dried under natural light for 10-12 hours, and then the solid-liquid separation is performed and the filtrate is collected; S2: adding EM bacteria and plant straw to the obtained filtrate, adjusting the temperature of the mixture to 40-45°C, and fermenting for 36-48 hours, followed by solid-liquid separation to obtain a fermentation liquid; S3: Thaw and activate Bacillus licheniformis, Bacillus subtilis, Pseudomonas chlororaphis, and Trichoderma viride strains respectively, streak the plates and culture them in a 28°C incubator for 48 h, take a loop of the cultured strains and place them in LB liquid culture medium, place them in a shaker at 28°C and 200 r / min for 24 h, and obtain seed solution; The seed liquid of the four strains was inoculated into NB liquid culture medium at an inoculation rate of 3%, the bottle volume was 200 mL, and the culture was shaken at 30°C and 200 r / min for 72 hours to obtain fermentation liquid; the four fermentation liquids were mixed at a mass ratio of 1:1:1:1 to obtain a composite microbial agent; S4: preparing a sunflower disk extract and combining it with sodium laureth sulfate in a mass ratio of 3:1 to form an accelerator; S5: The obtained fermentation liquid, composite microbial agent, promoter, potassium dihydrogen phosphate and trace elements are uniformly mixed according to weight ratio, and then packaged to obtain the final product.

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