Automatic batch embedding device and embedding method for biological tissue

Abstract

The present invention discloses an automatic batch embedding device and an embedding method for biological tissues, and relates to the field of embedding technology. The device comprises a loading device, a transporting and transferring device, an embedding device and a unloading device; the loading device comprises a first loading mechanism for loading an embedding box and a second loading mechanism for loading a metal mold; the transporting and transferring device comprises a clamping mechanism, a cover opening mechanism and a transport driving mechanism; the clamping mechanism comprises a first clamping mechanism for clamping a wax injection loading box and a mold base, a second clamping mechanism for clamping a sample and a third clamping mechanism for clamping a box body; the unloading device comprises a first unloading mechanism for unloading a cover body, a wax injection loading box and a loading support plate and a second unloading mechanism for unloading a metal mold and an embedding assembly. The present invention can automatically complete a large number of embedding tasks, save time and labor, and improve embedding efficiency.

Description

Technical Field

[0001] The invention relates to an embedding device and an embedding method, and in particular to an automatic batch embedding device and an embedding method for biological tissues. Background Art

[0002] Pathological diagnosis is a method of making a diagnosis by observing the tissue structure, cell morphology, color reaction, etc. of clinical samples. Pathological diagnosis is currently the most accurate diagnostic method and is usually used as the final diagnosis for most diseases, especially cancer. The preparation of pathological sections is an important part of pathological diagnosis. The preparation of pathological sections can be divided into several steps: sampling, fixation, dehydration, embedding, slicing, and staining. During the embedding process, a biological tissue embedding machine is required. The biological tissue embedding machine is a device that embeds tissue wax blocks after dehydration and wax immersion of human or animal and plant specimens for histological diagnosis or research.

[0003] Although the existing biological tissue embedding machine can embed the biological tissue after dehydration, the embedding process requires manual assistance. That is, during the embedding process, the biological tissue after dehydration is placed in the embedding box, and the operator first places the metal mold on the working table of the embedding machine, and then injects a layer of paraffin liquid into the metal mold through the embedding machine. Then the operator opens the embedding box and clamps the biological tissue into the metal mold with tweezers, so that the biological tissue is located above the paraffin liquid in the metal mold and in the middle of the metal mold; then the embedding machine injects the paraffin liquid into the metal mold; After the paraffin liquid reaches the predetermined liquid level line of the metal mold, the embedding machine stops discharging wax; the inspector puts the box body of the embedding box on top of the metal mold, and then the embedding machine injects paraffin liquid into the box body of the embedding box. Since the box body of the embedding box is a hollow structure, after the paraffin liquid in the metal mold solidifies, the box body will adhere to the wax block in the metal mold to facilitate subsequent demoulding; then the operator moves the metal mold together with the box body of the embedding box to the freezing table for rapid cooling, so that the paraffin liquid solidifies quickly; then demoulding is carried out, and the wax block after demoulding is connected to the box body of the embedding box to complete the embedding work.

[0004] However, the above-mentioned biological tissue embedding machine and the corresponding embedding method are to assist the operator to complete the embedding work of the biological tissue, that is, the operation of the operator is still the main one, and full automation cannot be achieved; therefore, although the above-mentioned biological tissue embedding machine can reduce the workload of the operator, when a large number of biological tissues need to be embedded, the workload of the operator is still huge, and under this huge workload, the operator is prone to physical and mental fatigue, which affects the work efficiency and may even cause work errors. Summary of the invention

[0005] The purpose of the present invention is to overcome the above-mentioned problems and provide an automatic batch embedding device for biological tissues, which can automatically complete a large number of embedding tasks, save time and labor, and help improve the embedding efficiency.

[0006] Another object of the present invention is to provide a method for automatic batch embedding of biological tissues.

[0007] The purpose of the present invention is achieved through the following technical solutions:

[0008] An automatic batch embedding device for biological tissues, comprising a loading device, a transporting and transferring device, an embedding device and a unloading device;

[0009] The feeding device comprises a first feeding mechanism for feeding the embedding box and a second feeding mechanism for feeding the metal mold; the first feeding mechanism comprises a bearing assembly for bearing the embedding box and a first feeding conveying mechanism for conveying the bearing assembly; the bearing assembly comprises a wax injection feeding box and a feeding supporting plate, wherein the wax injection feeding box is filled with liquid paraffin; a plurality of supporting avoidance holes are provided on the feeding supporting plate; the embedding box is placed on the feeding supporting plate, and the bottom of the box body of the embedding box passes downward through the supporting avoidance holes and contacts with the liquid paraffin in the wax injection feeding box; the second feeding mechanism comprises a mold base for bearing the metal mold and a second feeding conveying mechanism for conveying the mold base; a plurality of positioning and placing grooves are provided on the mold base;

[0010] The transport and transfer device comprises a clamping mechanism, a cover opening mechanism and a transport driving mechanism; the clamping mechanism comprises a first clamping mechanism for clamping the wax injection loading box and the mold base, a second clamping mechanism for clamping the sample in the box body and a third clamping mechanism for clamping the box body of the embedding box; the cover opening mechanism is used to open the cover body of the embedding box; the cover opening mechanism and the clamping mechanism are arranged on the transport driving mechanism;

[0011] The embedding device comprises a workbench, a freezing table and a wax supply mechanism, wherein the wax supply mechanism comprises a wax cylinder, a wax outlet pipe and a wax outlet nozzle; a plurality of wax outlet nozzles are provided and the layout is the same as that of the plurality of positioning and placing slots;

[0012] The unloading device comprises a first unloading mechanism for unloading the cover body of the embedding box, the wax injection loading box and the loading support plate, and a second unloading mechanism for unloading the metal mold and the embedding assembly.

[0013] A preferred embodiment of the present invention, wherein the first clamping mechanism comprises a first clamping mounting frame, a first clamping plate and a first clamping driving cylinder; two first clamping plates are provided and arranged horizontally opposite to each other; the first clamping driving cylinder is arranged on the first clamping mounting frame, and the first clamping driving cylinder is a double-rod cylinder and is respectively connected to the two first clamping plates.

[0014] A preferred embodiment of the present invention is that the second clamping mechanism is provided with multiple groups and the layout is the same as the layout of the multiple supporting avoidance holes, each group of the second clamping mechanism includes a second clamping plate and a second clamping drive cylinder; each group of the second clamping plates is provided with two and are arranged horizontally opposite to each other; the second clamping drive cylinder is arranged on the second clamping mounting frame, and the second clamping drive cylinder is a finger cylinder and is respectively connected to the two second clamping plates of the same group.

[0015] In a preferred embodiment of the present invention, the cover opening mechanism is provided with multiple groups and the layout is the same as the layout of the multiple supporting avoidance holes, and each group of cover opening mechanism includes a cover opening cover, a downward pressing unlocking component, a cover lifting component and a cover opening vacuum suction cup;

[0016] The cover opening cover is fixedly connected to the cover opening mounting frame;

[0017] The push-down unlocking assembly is provided with two groups and is relatively arranged on two sides of the cover opening cover, each group of push-down unlocking assembly includes a push-down plate, a push-down spring and a push-down guide rod; the push-down guide rod is vertically fixedly connected to the cover opening cover, and a push-down limit nut is provided at the bottom of the push-down guide rod; the push-down plate and the push-down spring are sleeved on the push-down guide rod, and the two ends of the push-down spring are respectively pressed against the push-down plate and the cover opening cover; the two sides of the box body are provided with a pressure-receiving part cooperating with the push-down plate;

[0018] The cover-lifting assembly is provided with two groups and is relatively arranged on the other two sides of the cover-lifting cover, each group of the cover-lifting assembly includes a cover-lifting mounting plate, a cover-lifting swinging member and a cover-lifting torsion spring, the cover-lifting mounting plate is connected to the cover-lifting cover, and the bottom of the cover-lifting mounting plate is provided with a cover-lifting mounting hole facing the embedding box; the cover-lifting swinging member is horizontally rotatably connected in the cover-lifting mounting hole, and the two ends of the cover-lifting swinging member are respectively a cover-lifting part and a transmission part with the rotation center as the boundary; the cover-lifting torsion spring is connected between the cover-lifting swinging member and the cover-lifting mounting plate;

[0019] The cover body and the box body are connected by a buckle structure, which includes a buckle hole and a hook, the hook is arranged on the cover body, and the bottom of the hook is provided with an inclined pressure-bearing surface; the buckle hole is arranged on the box body, and the hook passes through the buckle hole to fix the cover body and the box body together; the corresponding two side walls of the supporting avoidance hole of the loading support plate are provided with a pressing part for pressing the hook; when the embedding box is placed on the loading support plate, the hook is supported on the pressing part, and there is a downward space between the box body and the surface of the loading support plate; when the lower pressing plate presses the box body downward, the pressing part first acts on the hook to unlock, and then acts on the cover-lifting swinging piece to lift the cover, and then the cover body is adsorbed by the cover-opening vacuum suction cup.

[0020] Furthermore, a side surface of the cover body corresponding to the cover-lifting portion is provided with an anti-slip structure so that the cover-lifting portion of the cover-lifting swinging member can smoothly lift up the cover body.

[0021] Furthermore, the cover opening vacuum suction cup is slidably connected to the cover opening cover vertically via a mounting rod, and a buffer spring is sleeved on the mounting rod.

[0022] Furthermore, the third clamping mechanism includes multiple groups and the layout is the same as the layout of the multiple supporting avoidance holes, each group of the third clamping mechanism includes a third clamping plate and a clamping electromagnet; each group of the third clamping plates is provided with two and are horizontally arranged opposite to each other, and the third clamping plate is composed of the cover-lifting mounting plate; the cover-lifting mounting plate is connected to the cover-lifting cover through a horizontal sliding structure; each group of the clamping electromagnet is provided with two and are horizontally arranged opposite to each other on the cover-lifting cover, and the telescopic rod of the clamping electromagnet is connected to the corresponding third clamping plate. Through the above structure, during the cover-lifting work, the third clamping plate (cover-lifting mounting plate) remains stationary and plays a role of fixed installation; when the box body is clamped, the third clamping plate (cover-lifting mounting plate) is driven to move by the clamping electromagnet, thereby completing the transfer of the box body, which is very clever.

[0023] In a preferred embodiment of the present invention, the transport drive mechanism comprises a transverse transport drive mechanism and a vertical transport drive mechanism;

[0024] The transverse transport drive mechanism comprises a first transverse transport drive mechanism and a second transverse transport drive mechanism, wherein the driving direction of the first transverse transport drive mechanism is perpendicular to the driving direction of the second transverse transport drive mechanism;

[0025] The second transverse transport driving mechanism is arranged on the first transverse transport driving mechanism; and the vertical transport driving mechanism is arranged on the second transverse transport driving mechanism.

[0026] Furthermore, the first transverse transport drive mechanism includes a first transverse moving plate, a first transverse transport drive motor and a first transverse transport transmission assembly, the first transverse transport transmission assembly includes a first transverse transport screw and a first transverse transport screw nut; the first transverse transport screw nut is connected to the second transverse transport drive mechanism through the first transverse moving plate.

[0027] Furthermore, the second transverse transport drive mechanism includes a second transverse moving plate, a second transverse transport drive motor and a second transverse transport transmission assembly, and the second transverse transport transmission assembly includes a second transverse transport screw and a second transverse transport screw nut; the second transverse transport screw nut is connected to the vertical transport drive mechanism through the second transverse moving plate.

[0028] A preferred embodiment of the present invention, wherein the vertical transport drive mechanism includes a first vertical transport drive cylinder and a second vertical transport drive cylinder, the telescopic rod of the first vertical transport drive cylinder is connected to the first clamping mechanism; the telescopic rod of the second vertical transport drive cylinder is connected to the second clamping mechanism.

[0029] A method for automatic batch embedding of biological tissues, comprising the following steps:

[0030] Injecting liquid paraffin into a wax injection loading box, and placing a plurality of embedding boxes containing samples on a loading support plate of the wax injection loading box one by one, so that the bottom of the embedding box body and the samples are immersed in the liquid paraffin in the wax injection loading box, and then placing the wax injection loading box on a first loading conveying mechanism, and the first loading conveying mechanism conveys the wax injection loading box toward the embedding station;

[0031] Placing a plurality of metal molds one by one on the positioning and placing grooves corresponding to the mold base, and then placing the mold base on the second loading and conveying mechanism, and the second loading and conveying mechanism conveys the mold base toward the working table of the embedding device;

[0032] The first clamping mechanism is driven by the transport driving mechanism to approach the mold base, and the mold base is clamped by the first clamping mechanism. The mold base is then driven by the transport driving mechanism to move to the workbench of the embedding device, and the first clamping mechanism releases the mold base. At this time, the metal mold is located directly below the corresponding wax outlet nozzle.

[0033] The liquid paraffin is output into the metal mold through the wax supply mechanism;

[0034] The cover opening mechanism is driven by the transport driving mechanism to approach the embedding box, the cover body of the embedding box is opened by the cover opening mechanism, and the cover body is placed on the first unloading mechanism; the sample in the box body is clamped by the second clamping mechanism, and the transport driving mechanism drives the second clamping mechanism to transfer the sample to the metal mold;

[0035] The transport driving mechanism drives the third clamping mechanism to approach the cassette body of the embedding cassette, and the third clamping mechanism clamps the cassette body of the embedding cassette. The transport driving mechanism then drives the third clamping mechanism to transfer the cassette body to the corresponding metal mold on the workbench. The third clamping mechanism releases the cassette body and slightly presses the cassette body downward, so that the bottom of the cassette body extends into the inner cavity of the metal mold in place.

[0036] A large amount of liquid paraffin is output into the inner cavity of the metal mold through the wax supply mechanism, and the bottom of the box body is immersed in the liquid paraffin;

[0037] The first clamping mechanism is driven by the transport driving mechanism to approach the mold base, the mold base is clamped by the first clamping mechanism, and then the first clamping mechanism is driven by the transport driving mechanism to transfer the mold base to the freezing table;

[0038] After freezing, the box body and the metal mold form an embedding assembly; the embedding assembly is then transferred to the second unloading mechanism by the transport drive mechanism and the first clamping mechanism, and transported to the next station by the second unloading mechanism; before or after this, the wax injection loading box is transferred to the first unloading mechanism by the transport drive mechanism and the first clamping mechanism, and transported to the next station by the second unloading mechanism;

[0039] This completes one round of embedding work.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] The embedding device of the present invention can automatically complete a large number of embedding tasks, saving time and effort and being conducive to improving the embedding efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 It is a schematic diagram of the three-dimensional structure of the automatic batch embedding device for biological tissues of the present invention.

[0043] Figure 2 It is a schematic exploded view of the three-dimensional structure of the carrier assembly and the embedding box of the present invention.

[0044] Figure 3 It is a schematic exploded view of the three-dimensional structure of the metal mold and the mold base of the present invention.

[0045] Figure 4 It is a side view of the first clamping mechanism, the third clamping mechanism and the cover opening mechanism of the present invention.

[0046] Figure 5 It is a three-dimensional structural schematic diagram of the first clamping mechanism, the third clamping mechanism and the cover opening mechanism of the present invention.

[0047] Figure 6It is a partial three-dimensional structural schematic diagram of the cover opening mechanism of the present invention in the cover opening state.

[0048] Figure 7 It is a partial cross-sectional view of the cover opening mechanism of the present invention in the cover opening state.

[0049] Figure 8-Figure 10 for Figure 7 Zoomed in images of several different states of X in .

[0050] Fig.11 It is a schematic diagram of the three-dimensional structure of the second clamping mechanism of the present invention.

[0051] Fig.12 It is a schematic diagram of the three-dimensional structure of the transport drive mechanism of the present invention.

[0052] Fig.13 It is a schematic diagram of the three-dimensional structure of the embedding device of the present invention.

[0053] The numbers in the figure are:

[0054] 100-box body, 200-cover body, 300-metal mold, 1-first feeding mechanism, 2-second feeding mechanism, 3-wax injection feeding box, 4-feeding support plate, 4-1-support avoidance hole, 4-2-top pressure part, 5-mold base, 5-1-positioning placement groove, 6-first clamping mounting frame, 7-first clamping plate, 8-first clamping drive cylinder, 9-second clamping plate, 10-second clamping drive cylinder, 11-second clamping mounting frame, 12-opening cover, 13-opening vacuum suction cup, 14-opening mounting frame, 15-lower pressure plate, 16-lower pressure spring , 18-pressing limit nut, 19-cover mounting plate, 19-1-cover mounting hole, 20-cover swinging piece, 20-1-cover part, 20-2-transmission part, 21-clamping electromagnet, 22-first horizontal moving plate, 23-first horizontal transport driving motor, 24-second horizontal moving plate, 25-second horizontal transport driving motor, 26-first vertical transport driving cylinder, 27-second vertical transport driving cylinder, 28-workbench, 29-freezing table, 30-wax nozzle, 31-first unloading mechanism, 32-second unloading mechanism, 33-wax cylinder. DETAILED DESCRIPTION

[0055] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is further described below in conjunction with embodiments and drawings, but the embodiments of the present invention are not limited thereto.

[0056] See also Figure 1-Figure 3The biological tissue automatic batch embedding device of this embodiment includes a loading device, a handling and transfer device, an embedding device and a unloading device; the loading device includes a first loading mechanism 1 for loading the embedding box and a second loading mechanism 2 for loading the metal mold 300; the first loading mechanism 1 includes a bearing component for carrying the embedding box and a first loading and conveying mechanism for conveying the bearing component; the bearing component includes a wax injection loading box 3 and a loading support plate 4, and the wax injection loading box 3 is filled with liquid paraffin; a plurality of supporting avoidance holes 4-1 are opened on the loading support plate 4; the embedding box is placed on the loading support plate 4, and the bottom of the box body 100 of the embedding box passes downward through the supporting avoidance holes 4-1 and contacts with the liquid paraffin in the wax injection loading box 3; the second loading mechanism 2 includes a mold base 5 for carrying the metal mold 300 and a second loading and conveying mechanism for conveying the mold base 5; a plurality of positioning and placement grooves 5-1 are opened on the mold base 5.

[0057] Furthermore, the first feeding mechanism 1 and the second feeding mechanism 2 are both conveyor belt mechanisms, and the details can be referred to the prior art.

[0058] See also Figure 4-Figure 5 and Fig.11 The transport and transfer device includes a clamping mechanism, a cover opening mechanism and a transport driving mechanism; the clamping mechanism includes a first clamping mechanism for clamping the wax injection loading box 3 and the mold base 5, a second clamping mechanism for clamping the sample in the box body 100, and a third clamping mechanism for clamping the box body 100 of the embedding box; the cover opening mechanism is used to open the cover body 200 of the embedding box; the cover opening mechanism and the clamping mechanism are arranged on the transport driving mechanism.

[0059] See also Figure 4-Figure 5 The first clamping mechanism includes a first clamping mounting frame 6, a first clamping plate 7 and a first clamping driving cylinder 8; the first clamping plates 7 are provided with two and are arranged horizontally opposite to each other; the first clamping driving cylinder 8 is arranged on the first clamping mounting frame 6, and the first clamping driving cylinder 8 is a double-rod cylinder and is respectively connected to the two first clamping plates 7.

[0060] See also Fig.11 The second clamping mechanism is provided with multiple groups and the layout is the same as the layout of the multiple supporting avoidance holes 4-1, each group of the second clamping mechanism includes a second clamping plate 9 and a second clamping drive cylinder 10; each group of the second clamping plates 9 is provided with two and are horizontally arranged opposite to each other; the second clamping drive cylinder 10 is arranged on the second clamping mounting frame 11, and the second clamping drive cylinder 10 is a finger cylinder and is respectively connected to the two second clamping plates 9 of the same group.

[0061] See also Figure 4-Figure 10The cover opening mechanism is provided with multiple groups and the layout is the same as the layout of the multiple supporting avoidance holes 4-1, each group of cover opening mechanism includes a cover opening cover 12, a downward pressing unlocking component, a cover lifting component and a cover opening vacuum suction cup 13; the cover opening cover 12 is fixedly connected to the cover opening mounting frame 14; the downward pressing unlocking component is provided with two groups and is relatively arranged on two sides of the cover opening cover 12, each group of downward pressing unlocking components includes a downward pressing plate 15, a downward pressing spring 16 and a downward pressing guide rod; the downward pressing guide rod is vertically fixedly connected to the cover opening cover 12, and a downward pressing limit nut 18 is provided at the bottom of the downward pressing guide rod; the downward pressing unlocking component is provided with a downward pressing limit nut 18; the downward pressing unlocking component is provided with a downward pressing plate 15, a downward pressing spring 16 and a downward pressing guide rod; the downward pressing guide rod is vertically fixedly connected to the cover opening cover 12, and a downward pressing limit nut 18 is provided at the bottom of the downward pressing guide rod; the downward pressing unlocking component is provided with a downward pressing limit nut 18, and the downward pressing limit nut 18 is provided at the bottom of the downward pressing guide rod; the downward pressing limit nut 18 is provided at the bottom of the downward pressing limit nut 18; the downward pressing limit nut 18 is provided with a ... The pressing plate 15 and the pressing spring 16 are sleeved on the pressing guide rod, and the two ends of the pressing spring 16 are respectively pressed against the pressing plate 15 and the cover 12; the two sides of the box body 100 are provided with a pressure receiving part cooperating with the pressing plate 15; the cover opening assembly is provided with two groups and is relatively arranged on the other two sides of the cover opening cover 12, each group of cover opening assemblies includes a cover opening mounting plate 19, a cover opening swinging member 20 and a cover opening torsion spring (not shown in the figure), the cover opening mounting plate 19 is connected to the cover opening cover 12, and the bottom of the cover opening mounting plate 19 is provided with a cover opening mounting hole 19-1 facing the embedding box; the cover opening assembly The cover swing member 20 is connected to the cover installation hole 19-1 by transverse rotation, and the two ends of the cover swing member 20 are the cover lifting part 20-1 and the transmission part 20-2 respectively, with the rotation center as the boundary; the cover lifting torsion spring is connected between the cover swing member 20 and the cover installation plate 19; the cover body 200 and the box body 100 are connected by a buckle structure, and the buckle structure includes a buckle hole and a buckle hook, and the buckle hook is arranged on the cover body 200, and the bottom of the buckle hook is provided with an inclined pressure surface; the buckle hole is arranged on the box body 100, and the buckle hook passes through the buckle hole to connect the cover body 200 and the box body 1 00 are fixedly connected together; the corresponding two side walls of the supporting avoidance hole 4-1 of the loading support plate 4 are provided with a pressing part 4-2 for pressing the hook; when the embedding box is placed on the loading support plate 4, the hook is supported on the pressing part 4-2, and there is a downward movement space between the box body 100 and the surface of the loading support plate 4; when the lower pressing plate 15 presses the box body 100 downward, the pressing part 4-2 first acts on the hook to unlock, and then acts on the cover lifting swing member 20 to lift the cover, and then the cover opening vacuum suction cup 13 adsorbs the cover body 200.

[0062] Through the above structure, when preparing to open the cover, the entire cover opening mechanism moves to the top of the corresponding wax injection and loading box 3, and then moves downward to the corresponding cover body 200; first, the lower pressure plate 15 is pressed on the corresponding pressure-bearing part of the box body 100, so that the entire embedding box moves downward, and the downward pressure spring 16 at this time deforms and stores energy; during this period, since the hook is supported on the top pressure part 4-2, the top pressure part 4-2 acts on the inclined pressure-bearing surface of the hook. As the cover body 200 moves downward, the top pressure part 4-2 first drives the hook horizontally, so that the hook returns to the extension direction of the card hole, thereby achieving unlocking; at the same time, the transmission part 20-2 of the cover-lifting swing member 20 also presses downward on the top pressure part 4-2, and the top pressure part 4-2 drives the cover-lifting swing member 20 to rotate, so that the cover-lifting part 20-1 of the cover-lifting swing member 20 approaches and presses on the side of the cover body 200, as shown in FIG. Figure 8-Figure 9 At this time, the cover opening torsion spring is deformed and stores energy, and drives the cover opening swing member 20 to reset after the cover opening operation is completed; as the cover opening cover 12 continues to move downward, supported by the top pressing portion 4-2, the cover opening swing member 20 continues to rotate upward, thereby pushing the cover body 200 upward through the cover opening portion 20-1 (slight plastic deformation may occur during this period), such as Fig.10 , and then the cover body 200 is adsorbed by the cover opening vacuum suction cup 13; the entire cover opening mechanism is driven to move upward by the transport drive mechanism, and the cover opening vacuum suction cup 13 moves upward with the cover body 200; in this process, since there is still some pulling force between the cover body 200 and the box body 100, in order to prevent the box body 100 from moving upward with the cover body 200, in the initial upward stroke, the downward pressure spring 16 gradually recovers its deformation and releases potential energy, thereby pressing the lower pressure plate 15 downward. At this time, the lower pressure plate 15 and the box body 100 remain stationary, and the cover body 200 is completely separated from the box body 100 upward, and finally the lower pressure plate 15 moves upward, thereby completing the cover opening operation.

[0063] Furthermore, a side surface of the cover body 200 corresponding to the cover-lifting portion 20 - 1 is provided with an anti-slip structure so that the cover-lifting portion 20 - 1 of the cover-lifting swinging member 20 can smoothly lift up the cover body 200 .

[0064] Furthermore, the cover opening vacuum suction cup 13 is slidably connected to the cover opening cover 12 vertically via a mounting rod, and a buffer spring is sleeved on the mounting rod.

[0065] See also Figure 4-Figure 5The third clamping mechanism includes multiple groups and the layout is the same as the layout of the multiple supporting avoidance holes 4-1. Each group of the third clamping mechanism includes a third clamping plate and a clamping electromagnet 21; each group of the third clamping plates is provided with two and are horizontally arranged oppositely, and the third clamping plate is composed of the cover-lifting mounting plate 19; the cover-lifting mounting plate 19 is connected to the cover-lifting cover 12 through a horizontal sliding structure; each group of the clamping electromagnet 21 is provided with two and are horizontally arranged oppositely on the cover-lifting cover 12, and the telescopic rod of the clamping electromagnet 21 is connected to the corresponding third clamping plate. Through the above structure, during the cover-lifting work, the third clamping plate (cover-lifting mounting plate 19) remains stationary and plays a role of fixed installation; when the box body 100 is clamped, the third clamping plate (cover-lifting mounting plate 19) is driven to move by the clamping electromagnet 21, thereby completing the transfer of the box body 100, which is very clever.

[0066] The transport drive mechanism includes a transverse transport drive mechanism and a vertical transport drive mechanism; the transverse transport drive mechanism includes a first transverse transport drive mechanism and a second transverse transport drive mechanism, the driving direction of the first transverse transport drive mechanism is perpendicular to the driving direction of the second transverse transport drive mechanism; the second transverse transport drive mechanism is arranged on the first transverse transport drive mechanism; the vertical transport drive mechanism is arranged on the second transverse transport drive mechanism.

[0067] See also Fig.12 The first transverse transport driving mechanism includes a first transverse moving plate 22, a first transverse transport driving motor 23 and a first transverse transport transmission assembly, the first transverse transport transmission assembly includes a first transverse transport screw and a first transverse transport screw nut; the first transverse transport screw nut is connected to the second transverse transport driving mechanism through the first transverse moving plate 22.

[0068] See also Fig.12 The second transverse transport drive mechanism includes a second transverse moving plate 24, a second transverse transport drive motor 25 and a second transverse transport transmission assembly, and the second transverse transport transmission assembly includes a second transverse transport screw and a second transverse transport screw nut; the second transverse transport screw nut is connected to the vertical transport drive mechanism through the second transverse moving plate 24.

[0069] See also Fig.12 The vertical transport drive mechanism includes a first vertical transport drive cylinder 26 and a second vertical transport drive cylinder 27. The telescopic rod of the first vertical transport drive cylinder 26 is connected to the first clamping mechanism; the telescopic rod of the second vertical transport drive cylinder 27 is connected to the second clamping mechanism.

[0070] See also Figure 1 and Fig.13The embedding device includes a workbench 28, a freezing table 29 and a wax supply mechanism, and the wax supply mechanism includes a wax cylinder 33, a wax outlet pipe and a wax outlet nozzle 30; the wax outlet nozzle 30 is provided with multiple and the layout is the same as the layout of the multiple positioning placement slots 5-1; specifically, the embedding device of this embodiment can refer to the prior art.

[0071] The unloading device comprises a first unloading mechanism 31 for unloading the cover 200 of the embedding box, the wax injection loading box 3 and the loading support plate 4, and a second unloading mechanism 32 for unloading the metal mold 300 and the embedding assembly.

[0072] Furthermore, both the first unloading mechanism 31 and the second unloading mechanism 32 are conveyor belt mechanisms, and the details may refer to the prior art.

[0073] See also Figure 1-Figure 13 The biological tissue automatic batch embedding method of this embodiment comprises the following steps:

[0074] Liquid paraffin is injected into the wax injection loading box 3, and a plurality of embedding boxes containing samples are placed one by one on the loading support plate 4 of the wax injection loading box 3, so that the bottom of the box body 100 of the embedding box and the samples are immersed in the liquid paraffin in the wax injection loading box 3, and then the wax injection loading box 3 is placed on the first loading and conveying mechanism, and the wax injection loading box 3 is conveyed toward the embedding station by the first loading and conveying mechanism.

[0075] The plurality of metal molds 300 are placed one by one on the corresponding positioning grooves 5 - 1 of the mold base 5 , and then the mold base 5 is placed on the second loading and conveying mechanism, which conveys the mold base 5 toward the workbench 28 of the embedding device.

[0076] The first clamping mechanism is driven by the transport driving mechanism to approach the mold base 5, and the mold base 5 is clamped by the first clamping mechanism. The mold base 5 is then driven by the transport driving mechanism to move to the workbench 28 of the embedding device. The first clamping mechanism releases the mold base 5. At this time, the metal mold 300 is located directly below the corresponding wax outlet nozzle 30.

[0077] A small amount of liquid paraffin is output into the metal mold 300 by the wax supply mechanism.

[0078] The cover opening mechanism is driven by the transport driving mechanism to approach the embedding box, and the cover body 200 of the embedding box is opened by the cover opening mechanism, and the cover body 200 is placed on the first unloading mechanism 31; the sample in the box body 100 is clamped by the second clamping mechanism, and the transport driving mechanism drives the second clamping mechanism to transfer the sample to the metal mold 300.

[0079] The third clamping mechanism is driven by the transport driving mechanism to approach the box body 100 of the embedding box, and the box body 100 of the embedding box is clamped by the third clamping mechanism. Then, the third clamping mechanism is driven by the transport driving mechanism to transfer the box body 100 to the corresponding metal mold 300 on the workbench 28. The third clamping mechanism releases the box body 100 and slightly presses down the box body 100, so that the bottom of the box body 100 extends into the inner cavity of the metal mold 300 in place.

[0080] The liquid paraffin is output into the inner cavity of the metal mold 300 through the wax supply mechanism, and at this time, the bottom of the box body 100 is immersed in the liquid paraffin.

[0081] The first clamping mechanism is driven by the transport driving mechanism to approach the mold base 5 , and the mold base 5 is clamped by the first clamping mechanism. The first clamping mechanism is then driven by the transport driving mechanism to transfer the mold base 5 to the freezing table 29 .

[0082] After freezing, the box body 100 and the metal mold 300 form an embedded assembly; then the embedded assembly is transferred to the second unloading mechanism 32 through the transport drive mechanism and the first clamping mechanism, and is transported to the next station by the second unloading mechanism 32; before or after this, the wax injection loading box 3 is transferred to the first unloading mechanism 31 through the transport drive mechanism and the first clamping mechanism, and is transported to the next station by the second unloading mechanism 32.

[0083] This completes one round of embedding work.

[0084] The above is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited to the above content. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. An automatic batch embedding device for biological tissues, characterized in that: It includes a loading device, a handling and transferring device, an embedding device and a unloading device; The feeding device comprises a first feeding mechanism for feeding the embedding box and a second feeding mechanism for feeding the metal mold; the first feeding mechanism comprises a bearing assembly for bearing the embedding box and a first feeding conveying mechanism for conveying the bearing assembly; the bearing assembly comprises a wax injection feeding box and a feeding supporting plate, wherein the wax injection feeding box is filled with liquid paraffin; a plurality of supporting avoidance holes are provided on the feeding supporting plate; the embedding box is placed on the feeding supporting plate, and the bottom of the box body of the embedding box passes downward through the supporting avoidance holes and contacts with the liquid paraffin in the wax injection feeding box; the second feeding mechanism comprises a mold base for bearing the metal mold and a second feeding conveying mechanism for conveying the mold base; a plurality of positioning and placing grooves are provided on the mold base; The transport and transfer device comprises a clamping mechanism, a cover opening mechanism and a transport driving mechanism; the clamping mechanism comprises a first clamping mechanism for clamping the wax injection loading box and the mold base, a second clamping mechanism for clamping the sample in the box body and a third clamping mechanism for clamping the box body of the embedding box; the cover opening mechanism is used to open the cover body of the embedding box; the cover opening mechanism and the clamping mechanism are arranged on the transport driving mechanism; The embedding device comprises a workbench, a freezing table and a wax supply mechanism, wherein the wax supply mechanism comprises a wax cylinder, a wax outlet pipe and a wax outlet nozzle; a plurality of wax outlet nozzles are provided and the layout is the same as that of the plurality of positioning and placing slots; The unloading device comprises a first unloading mechanism for unloading the cover body of the embedding box, the wax injection loading box and the loading support plate, and a second unloading mechanism for unloading the metal mold and the embedding assembly.

2. The automatic batch embedding device for biological tissues according to claim 1, characterized in that: The first clamping mechanism includes a first clamping mounting frame, a first clamping plate and a first clamping drive cylinder; the first clamping plates are provided with two and are arranged horizontally opposite to each other; the first clamping drive cylinder is arranged on the first clamping mounting frame, and the first clamping drive cylinder is a double-rod cylinder and is respectively connected to the two first clamping plates.

3. The automatic batch embedding device for biological tissues according to claim 1, characterized in that: The second clamping mechanism is provided with multiple groups and the layout is the same as the layout of multiple supporting avoidance holes, each group of the second clamping mechanism includes a second clamping plate and a second clamping drive cylinder; each group of second clamping plates is provided with two and are arranged horizontally opposite to each other; the second clamping drive cylinder is arranged on the second clamping mounting frame, and the second clamping drive cylinder is a finger cylinder and is respectively connected to the two second clamping plates of the same group.

4. The automatic batch embedding device for biological tissues according to claim 1, characterized in that: The cover opening mechanism is provided with multiple groups and the layout is the same as the layout of the multiple supporting avoidance holes, and each group of cover opening mechanism includes a cover opening cover, a downward pressing unlocking component, a cover lifting component and a cover opening vacuum suction cup; The cover opening cover is fixedly connected to the cover opening mounting frame; The push-down unlocking assembly is provided with two groups and is relatively arranged on two sides of the cover opening cover, each group of push-down unlocking assembly includes a push-down plate, a push-down spring and a push-down guide rod; the push-down guide rod is vertically fixedly connected to the cover opening cover, and a push-down limit nut is provided at the bottom of the push-down guide rod; the push-down plate and the push-down spring are sleeved on the push-down guide rod, and the two ends of the push-down spring are respectively pressed against the push-down plate and the cover opening cover; the two sides of the box body are provided with a pressure-receiving part cooperating with the push-down plate; The cover-lifting assembly is provided with two groups and is relatively arranged on the other two sides of the cover-lifting cover, each group of the cover-lifting assembly includes a cover-lifting mounting plate, a cover-lifting swinging member and a cover-lifting torsion spring, the cover-lifting mounting plate is connected to the cover-lifting cover, and the bottom of the cover-lifting mounting plate is provided with a cover-lifting mounting hole facing the embedding box; the cover-lifting swinging member is horizontally rotatably connected in the cover-lifting mounting hole, and the two ends of the cover-lifting swinging member are respectively a cover-lifting part and a transmission part with the rotation center as the boundary; the cover-lifting torsion spring is connected between the cover-lifting swinging member and the cover-lifting mounting plate; The cover body and the box body are connected by a buckle structure, which includes a buckle hole and a hook, the hook is arranged on the cover body, and the bottom of the hook is provided with an inclined pressure-bearing surface; the buckle hole is arranged on the box body, and the hook passes through the buckle hole to fix the cover body and the box body together; the corresponding two side walls of the supporting avoidance hole of the loading support plate are provided with a pressing part for pressing the hook; when the embedding box is placed on the loading support plate, the hook is supported on the pressing part, and there is a downward space between the box body and the surface of the loading support plate; when the lower pressing plate presses the box body downward, the pressing part first acts on the hook to unlock, and then acts on the cover-lifting swinging piece to lift the cover, and then the cover body is adsorbed by the cover-opening vacuum suction cup.

5. The automatic batch embedding device for biological tissues according to claim 4, characterized in that: The side surface of the cover body corresponding to the cover-lifting portion is provided with an anti-slip structure.

6. The automatic batch embedding device for biological tissues according to claim 4, characterized in that: The cover-opening vacuum suction cup is slidably connected to the cover-opening cover vertically through a mounting rod, and a buffer spring is sleeved on the mounting rod.

7. The automatic batch embedding device for biological tissues according to claim 4, characterized in that: The third clamping mechanism includes multiple groups and the layout is the same as the layout of multiple supporting avoidance holes, each group of third clamping mechanisms includes a third clamping plate and a clamping electromagnet; each group of third clamping plates is provided with two and are horizontally oppositely arranged, and the third clamping plate is composed of the cover-lifting mounting plate; the cover-lifting mounting plate is connected to the cover-opening cover through a horizontal sliding structure; each group of clamping electromagnets is provided with two and are horizontally oppositely arranged on the cover-opening cover, and the telescopic rod of the clamping electromagnet is connected to the corresponding third clamping plate.

8. The automatic batch embedding device for biological tissues according to claim 1, characterized in that: The transport drive mechanism includes a horizontal transport drive mechanism and a vertical transport drive mechanism; The transverse transport drive mechanism comprises a first transverse transport drive mechanism and a second transverse transport drive mechanism, wherein the driving direction of the first transverse transport drive mechanism is perpendicular to the driving direction of the second transverse transport drive mechanism; The second transverse transport driving mechanism is arranged on the first transverse transport driving mechanism; and the vertical transport driving mechanism is arranged on the second transverse transport driving mechanism.

9. The automatic batch embedding device for biological tissues according to claim 8, characterized in that: The vertical transport drive mechanism includes a first vertical transport drive cylinder and a second vertical transport drive cylinder, the telescopic rod of the first vertical transport drive cylinder is connected to the first clamping mechanism; the telescopic rod of the second vertical transport drive cylinder is connected to the second clamping mechanism.

10. An embedding method applied to the biological tissue automatic batch embedding device according to any one of claims 1 to 9, characterized in that: The following steps are involved: Injecting liquid paraffin into a wax injection loading box, and placing a plurality of embedding boxes containing samples on a loading support plate of the wax injection loading box one by one, so that the bottom of the embedding box body and the samples are immersed in the liquid paraffin in the wax injection loading box, and then placing the wax injection loading box on a first loading conveying mechanism, and the first loading conveying mechanism conveys the wax injection loading box toward the embedding station; Placing a plurality of metal molds one by one on the positioning and placing grooves corresponding to the mold base, and then placing the mold base on the second loading and conveying mechanism, and the second loading and conveying mechanism conveys the mold base toward the working table of the embedding device; The first clamping mechanism is driven by the transport driving mechanism to approach the mold base, and the mold base is clamped by the first clamping mechanism. The mold base is then driven by the transport driving mechanism to move to the workbench of the embedding device, and the first clamping mechanism releases the mold base. At this time, the metal mold is located directly below the corresponding wax outlet nozzle. The liquid paraffin is output into the metal mold through the wax supply mechanism; The cover opening mechanism is driven by the transport driving mechanism to approach the embedding box, the cover body of the embedding box is opened by the cover opening mechanism, and the cover body is placed on the first unloading mechanism; the sample in the box body is clamped by the second clamping mechanism, and the transport driving mechanism drives the second clamping mechanism to transfer the sample to the metal mold; The transport driving mechanism drives the third clamping mechanism to approach the cassette body of the embedding cassette, and the third clamping mechanism clamps the cassette body of the embedding cassette. The transport driving mechanism then drives the third clamping mechanism to transfer the cassette body to the corresponding metal mold on the workbench. The third clamping mechanism releases the cassette body and slightly presses the cassette body downward, so that the bottom of the cassette body extends into the inner cavity of the metal mold in place. Liquid paraffin is output into the inner cavity of the metal mold through the wax supply mechanism, and the bottom of the box body is immersed in the liquid paraffin; The first clamping mechanism is driven by the transport driving mechanism to approach the mold base, the mold base is clamped by the first clamping mechanism, and then the first clamping mechanism is driven by the transport driving mechanism to transfer the mold base to the freezing table; After freezing, the box body and the metal mold form an embedding assembly; the embedding assembly is then transferred to the second unloading mechanism by the transport drive mechanism and the first clamping mechanism, and transported to the next station by the second unloading mechanism; before or after this, the wax injection loading box is transferred to the first unloading mechanism by the transport drive mechanism and the first clamping mechanism, and transported to the next station by the second unloading mechanism; This completes one round of embedding work.

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