Preparation technology of hippocampus peptide beverage

Abstract

The invention relates to the technical field of beverage preparation, in particular to a hippocampal peptide beverage preparation technology which comprises the preparation steps of hippocampal peptide preparation, ginseng extracting solution preparation, raw material mixing and stirring, homogenizing, filtering and cooling. According to the present invention, the hippocampus peptide is adopted to prepare the beverage, such that the beverage is convenient to eat compared with the traditional eating mode of the hippocampus in the original form, the development space of the hippocampus industry can be easily expanded, and the hippocampus is pretreated by using the ultrasonic wave-low temperature vacuum cooking mode so as to improve the hydrolysis degree and the polypeptide recovery rate of the defatted hippocampus powder; the hippocampus peptide beverage has the advantages that the hippocampus medicinal material is fully utilized, the beta-cyclodextrine method is adopted to remove fishy smell, and the traditional Chinese medicine ginseng extract is added, so that the hippocampus peptide beverage is relatively comprehensive in nutrition and has the characteristics of low sugar, low fat, good taste and high nutritional value, and meanwhile, the hippocampus peptide beverage is relatively good in stability and does not have fishy smell returning and flocculation phenomena after being placed for a long time.

Description

Technical Field

[0001] The invention relates to the technical field of beverage preparation, in particular to a preparation process of a hippocampal peptide beverage. Background Art

[0002] Marine biological peptides can regulate the body's functions, repair damaged cells, and enhance the body's immunity. They have a series of effects such as anti-fatigue and anti-aging. They can also lower blood sugar, blood lipids, and blood pressure. They can also be anti-inflammatory, anti-fatigue, and enhance the body's immunity. They are also very beneficial for delaying aging. Among them, seahorse is a precious marine Chinese medicinal material with high medicinal and health value. Existing seahorse products are usually used in the form of original dried seahorses for cooking soups, etc., which are more troublesome to eat. In addition, marine peptide beverage products on the market usually have anti-fishy smell, flocculation and other phenomena, which have a relatively large impact on the flavor of the beverage. Summary of the invention

[0003] The object of the present invention is to provide a preparation process of a hippocampal peptide beverage to solve the problems raised in the above background technology.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] A preparation process of a hippocampal peptide beverage includes the following steps: preparing hippocampal peptide, preparing ginseng extract, mixing and stirring raw materials, homogenizing, filtering, and cooling. The specific steps are as follows:

[0006] Step (1) Preparation of hippocampal peptide: preparing hippocampal peptide powder using artificially cultured frozen American hippocampus linearis;

[0007] Step (ii) preparing ginseng extract: preparing ginseng extract powder using ginseng;

[0008] Step (iii) mixing and stirring the raw materials: according to the following ingredients per 100 grams of beverage: 1-3 g of hippocampal peptide powder, 0.7-1.5 g of ginseng extract powder, 0.3-0.5 g of functional trace elements (bio-selenium, zinc lactate, calcium lactate), 0.16-0.20 g of vitamin C, 1.2-1.7 g of flavored whey protein, 0.05 g of β-cyclodextrin, 0.05 g of sodium carboxymethyl cellulose, 0.01 g of pectin, and the rest being distilled water, after the raw materials are filled in order according to the proportion, they are put into a stirring structure for mixing and stirring;

[0009] Step (iv) homogenizing: homogenizing the mixed solution using a homogenizer;

[0010] Step (V) Filtration: Filter the mixed solution using a sterile filter;

[0011] Step (six) Cooling: Place the filtered mixed solution in a refrigerator and allow to cool to 4 degrees Celsius.

[0012] Furthermore, in step (iii), the flavored whey protein is selected from strawberry whey powder, blueberry whey powder, and oolong tea water-soluble powder to prepare different flavors.

[0013] Furthermore, the specific preparation steps of the hippocampal peptide in step (i) include preparation of defatted hippocampal powder - ultrasonic-low-temperature vacuum cooking treatment - enzymatic hydrolysis - centrifugal separation - decolorization - ultrafiltration - desalting - drying, and the specific steps are as follows:

[0014] Step (1) Preparation of defatted hippocampus powder: freeze-dry frozen American hippocampus at -40°C for 48 hours, crush it with a crusher, and then remove the fatty acids of the hippocampus with supercritical fluid extraction of carbon dioxide to obtain defatted hippocampus powder;

[0015] Step (2) Ultrasonic-low-temperature vacuum cooking treatment: dissolving the defatted seahorse powder obtained above in distilled water at a weight ratio of 1:15 between the material and the liquid, stirring magnetically for 30 minutes, and then performing ultrasonic treatment. The seahorse powder solution is sealed and subjected to low-temperature vacuum cooking;

[0016] Step (3) Enzymatic hydrolysis: add papain and alkaline protease in an amount of 5% of defatted seahorse powder to the above solution, respectively, at pH 9.0, at a hydrolysis temperature of 50° C., for 6 h, then inactivate the enzyme at 100° C. for 15 min, and then cool to room temperature after inactivation;

[0017] Step (4) centrifugation: centrifuging the above enzymatic hydrolysate at 9000 rpm for 20 min, and concentrating the obtained supernatant to 1 / 5 of the original volume to obtain a hippocampal peptide concentrate;

[0018] Step (5) decolorization: the hippocampal peptide concentrate obtained in step (4) is added into a constant temperature stirring device, and then activated carbon and diatomaceous earth are added, and the mixture is stirred at 70° C. for 20 minutes by the constant temperature stirring device for decolorization;

[0019] Step (6) ultrafiltration: ultrafiltration of the decolorized solution using a 20 kDa ultrafiltration membrane to obtain two hippocampal peptides with different molecular weight ranges: PH-I (molecular weight greater than 20 kDa) and PH-II (molecular weight less than 20 kDa);

[0020] Step (7) Desalination: Desalting PH-II using membrane separation technology;

[0021] Step (8) Drying: The desalted peptide solution is spray dried to obtain the target hippocampal peptide powder (molecular weight less than 20 kDa).

[0022] Furthermore, in step (2), the ultrasonic treatment time is 20 min, the ultrasonic power is 600 W, the ultrasonic temperature is 50° C., the cooking temperature is 55° C., the cooking time is 20 min, and the vacuum degree is 0.05 MPa.

[0023] Furthermore, the constant temperature stirring device in step (5) comprises:

[0024] A containing module, used for placing hippocampal peptide concentrate, the containing module comprising a mixing drum, a plurality of supporting columns are fixedly connected to the bottom surface of the mixing drum, and a material discharge mechanism is arranged on the top of the inner side surface of the mixing drum;

[0025] A stirring module is arranged inside a mixing drum, the stirring module comprising a central tube, the bottom of the central tube side surface is rotatably connected to the inner side surface of the mixing drum bottom, the top and bottom of the central tube side surface are connected to two connecting tubes, the corresponding connecting tubes on both sides of the central tube are provided with heat conducting tubes, the top and bottom of the inner side surface of the heat conducting tube are rotatably connected to the adjacent connecting tube side surface, the side surface of the heat conducting tube is rotatably connected to two connecting tubes, the bottom of the side surface of the connecting tube is provided with an open groove, and a guide plate is fixedly connected to the corresponding open groove on the side surface of the connecting tube.

[0026] Furthermore, the containing module further comprises:

[0027] A drive box is fixedly connected to the bottom surface of the mixing drum, the bottom of the side surface of the central tube is rotatably connected to the inner side surface of the drive box, the inner bottom surface of the drive box is fixedly connected to a drive motor, the output end of the drive motor is drivingly connected to a conveying shaft rotatably connected to the inner top surface of the drive box, two mutually meshing transmission gears are arranged inside the drive box, the side surface of the conveying shaft is fixedly sleeved with one transmission gear, and the bottom of the side surface of the central tube is fixedly sleeved with another transmission gear, and the bottom surface of the drive box is fixedly connected to a conveying pipe rotatably connected to the bottom end of the central tube;

[0028] The connecting pipe is rotatably connected to the top of the central pipe, the bottom end of the connecting pipe is fixedly connected to a fixing frame fixedly connected to the inner side of the mixing drum, and the inner side of the fixing frame is rotatably connected to the top of the central pipe.

[0029] Preferably, a conveying frame is provided at the top inner side of the connecting cylinder, a partition plate slidably connected to the inner side surface of the connecting cylinder is fixedly connected at the bottom side of the conveying frame side, a rotating cylinder slidably connected to the side surface of the heat conduction tube is rotatably connected to the inner side surface of the conveying frame, a plurality of toggle plates are fixedly connected to the side surface of the rotating cylinder, a plurality of connecting grooves are provided on the bottom surface of the conveying frame, a strip groove is provided on the side surface of the conveying frame away from the opening groove, a positioning cylinder is fixedly connected to the top inner side surface of the connecting cylinder, the top surface of the positioning cylinder located at the bottom is fixedly connected to the corresponding bottom surface of the connecting cylinder, a sliding cylinder is slidably connected to the bottom inner side surface of the positioning cylinder, and the bottom surface of the sliding cylinder is fixedly connected to the top surface of the conveying frame.

[0030] Furthermore, two round rods are fixedly connected to the top surface of the partition, the top ends of the two round rods at the bottom are fixedly connected to the corresponding bottom surfaces of the partition, a screw-on ring is provided on the top of the heat pipe, and the top of the side surface of the screw-on ring is rotatably connected to a positioning ring fixedly connected to the top ends of the two adjacent round rods, and the top of the side surface of the positioning tube at the top is provided with a thread screwed together with the inner side surface of the screw-on ring.

[0031] Preferably, a connecting gear is fixedly sleeved on the top of the side of the heat conducting pipe;

[0032] The containing module further comprises:

[0033] A connecting ring is arranged on the top of the inner side of the mixing drum, the inner side of the connecting ring is rotatably connected to the side surfaces of the two heat conducting tubes, the bottom surface of the connecting ring is fixedly connected to the top surfaces of the two adjacent positioning drums, the connecting gear is located inside the connecting ring, and the bottom surface of the connecting ring is fixedly connected to a pushing ring;

[0034] The fixing ring is rotatably connected to the inner side of the connecting ring. The fixing ring side is fixedly connected to a plurality of fixing rods fixedly connected to the inner side of the mixing drum. The inner side of the fixing ring is provided with a gear ring meshing with two connecting gears.

[0035] Furthermore, the blanking mechanism comprises:

[0036] Guide frame;

[0037] Two limit frames are respectively arranged at the inner side and the outer side of the guide frame;

[0038] Two fixing seats are fixedly connected to the bottom surfaces of the guide frame and the limit frame, and the side surfaces of the fixing seats are fixedly connected to the inner side surfaces of the mixing drum;

[0039] A push plate is arranged on the top surface of the guide frame, and the side surface of the push plate is fixedly connected to the side surface of the push ring;

[0040] The two inclined panels are both fixedly connected to the bottom surface of the push plate, and the inclined panels are located between the guide frame and the corresponding limit frame.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] 1. By using hippocampal peptides to prepare beverages, compared with the traditional way of eating seahorses in their original form, it is more convenient to eat, which is conducive to expanding the development space of the seahorse industry. The seahorses are pre-treated by ultrasonic-low-temperature vacuum cooking to increase the hydrolysis degree and peptide recovery rate of defatted seahorse powder, which is conducive to making full use of seahorse medicinal materials. The β-cyclodextrin method is used to remove the fishy smell, and the Chinese medicine ginseng extract is added. The nutrition is more comprehensive, with the characteristics of low sugar, low fat, good taste and high nutritional value. At the same time, the stability of the hippocampal peptide beverage is relatively good, and no anti-fishy smell or flocculation phenomenon has occurred in 60 days.

[0043] 2. A conveying frame is provided on the top of the inner side of the connecting cylinder, and the hippocampal peptide concentrate can be placed in the mixing cylinder. Then, activated carbon and diatomaceous earth powder can be added to the mixing cylinder. At this time, the heating medium can be conveyed to the center tube through the conveying pipe. The heating medium can flow through the connecting tube and the heat conducting tube. Then, the center tube can be rotated to stir the hippocampal peptide concentrate with the connecting cylinder. During the stirring process, the concentrated liquid can flow quickly in the connecting cylinder through the rotation of the rotating cylinder. In this way, the temperature of the heating medium can be set higher, thereby quickly heating the concentrated liquid, and the contact time between the concentrated liquid and the surface of the connecting cylinder is short, thereby avoiding excessive heating of the concentrated liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is a process flow chart of the preparation of a hippocampal peptide beverage of the present invention;

[0045] Figure 2 This is a process flow chart for preparing hippocampal peptide powder in the present invention;

[0046] Figure 3 It is a schematic diagram of the overall structure of the constant temperature stirring device in the present invention;

[0047] Figure 4 It is a schematic diagram of the internal structure of the mixing drum in the present invention;

[0048] Figure 5 It is a schematic diagram of the internal structure of the drive box in the present invention;

[0049] Figure 6 It is a schematic diagram of the connecting ring structure in the present invention;

[0050] Figure 7 It is a schematic diagram of the internal structure of the connecting ring in the present invention;

[0051] Figure 8 It is a schematic diagram of the structure of the material feeding mechanism in the present invention;

[0052] Fig. 9 It is a schematic diagram of the internal structure of the guide frame in the present invention;

[0053] Fig.10 It is a schematic diagram of the structure of the push plate in the present invention;

[0054] Fig.11 It is a schematic diagram of the structure of the stirring module in the present invention;

[0055] Fig.12 It is a schematic diagram of the internal structure of the connecting tube in the present invention;

[0056] Fig.13 It is a schematic diagram of the internal structure of the conveying frame in the present invention.

[0057] In the figure: 100, containing module; 110, mixing drum; 111, supporting column; 120, driving box; 121, conveying pipe; 122, driving motor; 123, conveying shaft; 124, transmission gear; 130, connecting pipe; 131, fixing frame; 140, connecting ring; 141, pushing ring; 150, fixing ring; 151, fixing rod; 152, gear ring; 160, unloading mechanism; 161, guiding frame; 162, limiting frame; 163, fixing seat; 164, pushing plate; 165. Inclined plate; 200. Stirring module; 210. Central tube; 211. Connecting tube; 220. Heat conducting tube; 221. Connecting gear; 230. Connecting cylinder; 231. Guide plate; 232. Opening groove; 240. Conveying frame; 241. Partition plate; 242. Round rod; 243. Rotating cylinder; 244. Toggle plate; 245. Connecting groove; 246. Strip groove; 250. Positioning cylinder; 251. Sliding cylinder; 252. Thread; 260. Screw-on ring; 261. Positioning ring. DETAILED DESCRIPTION

[0058] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0059] See also Figure 1 In an embodiment of the present invention, a preparation process of a hippocampal peptide beverage includes the steps of preparing hippocampal peptides, preparing ginseng extract, mixing and stirring raw materials, homogenizing, filtering, and cooling. The specific steps are as follows:

[0060] Step (1) Preparation of hippocampal peptide: preparing hippocampal peptide powder using artificially cultured frozen American hippocampus linearis;

[0061] Step (ii) preparing ginseng extract: preparing ginseng extract powder using ginseng;

[0062] Step (iii) mixing and stirring the raw materials: according to the following ingredients per 100 grams of beverage: 1-3 g of hippocampal peptide powder, 0.7-1.5 g of ginseng extract powder, 0.3-0.5 g of functional trace elements (bio-selenium, zinc lactate, calcium lactate), 0.16-0.20 g of vitamin C, 1.2-1.7 g of flavored whey protein, 0.05 g of β-cyclodextrin, 0.05 g of sodium carboxymethyl cellulose, 0.01 g of pectin, and the rest being distilled water, after the raw materials are filled in order according to the proportion, they are put into a stirring structure for mixing and stirring;

[0063] Among them, the flavored whey protein can be prepared into different flavors using strawberry whey powder, blueberry whey powder, oolong tea water-soluble powder, etc.

[0064] Step (iv) homogenizing: homogenizing the mixed solution using a homogenizer;

[0065] Step (V) Filtration: Filter the mixed solution using a 0.25 μm sterile filter;

[0066] Step (six) Cooling: Place the filtered mixed solution in a refrigerator and allow to cool to 4 degrees Celsius.

[0067] Specifically, β-cyclodextrin is used to remove the fishy smell of beverages. Single-factor experiments show that the fishy smell removal effect increases with the increase of its concentration, but the maximum limit of β-cyclodextrin in beverages is 0.5 mg / mL. Therefore, the optimal addition amount of β-cyclodextrin in the beverage in this formula is 0.5 mg / mL. Sodium carboxymethyl cellulose and pectin are used as stabilizers. Orthogonal experiments show that the composite stabilizer enhances the stability of the beverage, but too much addition will affect the color of the beverage. The optimal addition amounts of the composite stabilizer are finally selected as 0.05% sodium carboxymethyl cellulose and 0.01% pectin.

[0068] Compared with the traditional way of eating seahorses in their original form, beverages prepared with hippocampal peptides are more convenient to consume, which is conducive to expanding the development space of the seahorse industry. The β-cyclodextrin method is used to remove the fishy smell, and the Chinese medicine ginseng extract is added. The nutrition is more comprehensive, with the characteristics of low sugar, low fat, good taste, and high nutritional value. The hippocampal peptide beverage has a uniform texture, no precipitation and stratification, a light fishy smell and seafood flavor, and a delicate taste. At the same time, the hippocampal peptide beverage has relatively good stability, and no anti-fishy smell or flocculation occurs after being placed at 4°C for 60 days.

[0069] Embodiment 1

[0070] like Figure 2 As shown, in this embodiment, the specific preparation steps of hippocampal peptide in step (a) include preparation of defatted hippocampal powder - ultrasonic-low temperature vacuum cooking treatment - enzymatic hydrolysis - centrifugal separation - decolorization - ultrafiltration - desalting - drying, and the specific steps are as follows:

[0071] Step (1) Preparation of defatted seahorse powder: freeze-drying artificially cultured frozen American hippocampus at -40°C for 48 hours, crushing it with a crusher, and then removing the seahorse fatty acids with carbon dioxide supercritical fluid extraction technology to obtain defatted seahorse powder;

[0072] Step (2) Ultrasonic-low-temperature vacuum cooking treatment: dissolving the defatted seahorse powder obtained above in distilled water at a weight ratio of 1:15 between the material and the liquid, stirring magnetically for 30 minutes, and then performing ultrasonic treatment. The seahorse powder solution is sealed and subjected to low-temperature vacuum cooking;

[0073] Among them, the ultrasonic treatment time is 20min, the ultrasonic power is 600W, the ultrasonic temperature is 50℃, the cooking temperature is 55℃, the cooking time is 20min, and the vacuum degree is 0.05MPa;

[0074] Step (3) Enzymatic hydrolysis: add papain and alkaline protease in an amount of 5% of defatted seahorse powder to the above solution, respectively, at pH 9.0, at a hydrolysis temperature of 50° C., for 6 h, then inactivate the enzyme at 100° C. for 15 min, and then cool to room temperature after inactivation;

[0075] Step (4) centrifugation: centrifuging the above enzymatic hydrolysate at 9000 rpm for 20 min, and concentrating the obtained supernatant to 1 / 5 of the original volume to obtain a hippocampal peptide concentrate;

[0076] Step (5) decolorization: the hippocampal peptide concentrate obtained in step (4) is added into a constant temperature stirring device, and then activated carbon and diatomaceous earth are added, and the mixture is stirred at 70° C. for 20 minutes by the constant temperature stirring device for decolorization;

[0077] Step (6) ultrafiltration: ultrafiltration of the decolorized solution using a 20 kDa ultrafiltration membrane to obtain two hippocampal peptides with different molecular weight ranges: PH-I (molecular weight greater than 20 kDa) and PH-II (molecular weight less than 20 kDa);

[0078] Step (7) Desalination: Desalting PH-II using membrane separation technology;

[0079] Step (8) Drying: The desalted peptide solution is spray dried to obtain the target hippocampal peptide powder (molecular weight less than 20 kDa).

[0080] In the specific implementation, the amount of defatted seahorse powder in each group used in the experiment was 100g. The results showed that ultrasonic-low-temperature vacuum cooking treatment can significantly improve the hydrolysis degree of defatted seahorse powder (P<0.01), from 9.31% without treatment to 13.11%. The total amount of polypeptides recovered by PH-I and PH-II was 62.3±7.9g, close to the crude protein content of 68.2%, which is higher than the untreated group of 55.4±8.2g, which is conducive to the full utilization of seahorse medicinal materials.

[0081] like Figure 2-11 As shown, in this embodiment, the constant temperature stirring device in step (5) includes a containing module 100 and a stirring module 200;

[0082] The containing module 100 is used to place the hippocampal peptide concentrate. The containing module 100 includes a mixing drum 110. A heating layer can be provided on the side wall of the mixing drum 110. By providing a heating structure in the heating interlayer, the concentrated liquid in the mixing drum 110 is heated and kept warm. A plurality of supporting columns 111 are fixedly connected to the bottom surface of the mixing drum 110. The supporting columns 111 can support the mixing drum 110. A feeding mechanism 160 is provided on the top of the inner side of the mixing drum 110.

[0083] The stirring module 200 is arranged inside the mixing drum 110. The stirring module 200 includes a central tube 210. The central tube 210 is solid in the middle, and the top and bottom are hollow tubular structures. The bottom of the side of the central tube 210 is rotatably connected to the inner side of the bottom of the mixing drum 110. The top and bottom of the side of the central tube 210 are connected to two connecting tubes 211. Heat conducting pipes 220 are arranged corresponding to the connecting tubes 211 on both sides of the central tube 210. The top and bottom of the inner side of the heat conducting pipe 220 are rotatably connected to the side of the adjacent connecting tube 211. The side of the heat conducting pipe 220 is rotatably connected to two connecting tubes 230. The bottom of the side of the connecting tube 230 is provided with an open groove 232. The side of the connecting tube 230 is fixedly connected with a guide plate 231 corresponding to the open groove 232.

[0084] In a specific implementation, after the concentrated liquid is placed in the mixing drum 110, the powdered activated carbon and diatomaceous earth can be added into the mixing drum 110 through the feeding mechanism 160. At this time, the concentrated liquid can be heated by the heating structure of the side wall of the mixing drum 110, and the heating medium can be transported to the center tube 210 at the same time. The heating medium will first flow into the bottom connecting tube 211, then enter the bottom of the heat conducting tube 220, and then flow to the top connecting tube 211 through the heat conducting tube 220, and finally flow from the top connecting tube 211 into the top of the center tube 210, and then the center tube 210 can be heated. The core tube 210 rotates, so that the concentrated liquid can be stirred through the connecting tube 211 and the connecting tube 230, thereby decolorizing the concentrated liquid. During stirring, the concentrated liquid can flow into the open groove 232 along the guide plate 231, so that the concentrated liquid can flow through the surface of the heat-conducting tube 220 located in the connecting tube 230, and the concentrated liquid is further heated by the heat-conducting tube 220, which is beneficial to improving the heating effect. Insulating materials or insulating coatings are arranged inside the core tube 210 and the connecting tube 211 to avoid heating the concentrated liquid through the core tube 210 and the connecting tube 211.

[0085] Embodiment 2

[0086] Based on the first embodiment, Figure 4-5 As shown, in this embodiment, the containing module 100 further includes a driving box 120 and a connecting pipe 130;

[0087] The drive box 120 is fixedly connected to the bottom surface of the mixing drum 110, and the bottom of the side surface of the central tube 210 is rotatably connected to the inner side surface of the drive box 120. The inner bottom surface of the drive box 120 is fixedly connected with a drive motor 122, and the output end of the drive motor 122 is transmission-connected with a conveying shaft 123 rotatably connected to the inner top surface of the drive box 120. Two mutually meshing transmission gears 124 are arranged inside the drive box 120, and the side surface of the conveying shaft 123 is fixedly sleeved with one transmission gear 124, and the bottom of the side surface of the central tube 210 is fixedly sleeved with the other transmission gear 124. The bottom surface of the drive box 120 is fixedly connected with a conveying pipe 121 rotatably connected to the bottom end of the central tube 210, and the connecting pipe 130 is rotatably connected to the top end of the central tube 210. The bottom end of the connecting pipe 130 is fixedly connected with a fixing frame 131 fixedly connected to the inner side surface of the mixing drum 110, and the fixing frame 131 can support the connecting pipe 130, and the inner side surface of the fixing frame 131 is rotatably connected to the top end of the central tube 210.

[0088] During specific implementation, the driving motor 122 can drive the conveying shaft 123 to rotate, and the conveying shaft 123 can drive the central tube 210 to rotate through the transmission gear 124, so that the connecting tube 211 and the connecting tube 230 stir the concentrated liquid, and the central tube 210 can rotate relative to the conveying tube 121 and the connecting tube 130, and the heating medium can be transported to the inside of the central tube 210 through the conveying tube 121, and the heating medium at the top of the central tube 210 can be discharged from the connecting tube 130.

[0089] like Figure 6-13 As shown, in this embodiment, a conveying frame 240 is provided at the top of the inner side of the connecting cylinder 230, a baffle 241 which is slidably connected to the inner side of the connecting cylinder 230 is fixedly connected at the bottom of the side of the conveying frame 240, a rotating cylinder 243 which is slidably connected to the side of the heat conducting pipe 220 is rotatably connected to the inner side of the conveying frame 240, a plurality of toggle plates 244 are fixedly connected to the side of the rotating cylinder 243, a plurality of connecting grooves 245 are provided on the bottom surface of the conveying frame 240, a strip groove 246 is provided on the side of the conveying frame 240 which is away from the opening groove 232, a positioning cylinder 250 is fixedly connected to the top of the inner side of the connecting cylinder 230, and the top of the positioning cylinder 250 which is located at the bottom The surface is fixedly connected with the bottom surface of the corresponding connecting cylinder 230, the bottom positioning cylinder 250 can connect the two connecting cylinders 230, and the top positioning cylinder 250 can connect the connecting cylinder 230 to the bottom surface of the connecting ring 140, so as to prevent the self-rotation of the connecting cylinder 230. The bottom of the inner side of the positioning cylinder 250 is slidably connected with a sliding cylinder 251, and the bottom surface of the sliding cylinder 251 is fixedly connected with the top surface of the conveying frame 240. The sliding cylinder 251 only slides up and down along the inside of the positioning cylinder 250, and the partition 241 only slides up and down along the inside of the connecting cylinder 230, so that the self-rotation of the conveying frame 240 can be limited. The top of the side of the heat conducting pipe 220 is fixedly sleeved with a connecting gear 221;

[0090] The containing module 100 further includes a connecting ring 140 and a fixing ring 150;

[0091] The connecting ring 140 is arranged on the top of the inner side of the mixing drum 110, the inner side surface of the connecting ring 140 is rotatably connected to the side surfaces of the two heat-conducting tubes 220, the bottom surface of the connecting ring 140 is fixedly connected to the top surfaces of the two adjacent positioning tubes 250, the connecting gear 221 is located inside the connecting ring 140, the bottom surface of the connecting ring 140 is fixedly connected to the pushing ring 141, the fixing ring 150 is rotatably connected to the inner side surface of the connecting ring 140, the side surface of the fixing ring 150 is fixedly connected to a plurality of fixing rods 151 fixedly connected to the inner side surface of the mixing drum 110, and the inner side surface of the fixing ring 150 is provided with a gear ring 152 meshing with the two connecting gears 221.

[0092] In a specific implementation, the rotating cylinder 243 only slides up and down along the heat conducting tube 220, so that when the heat conducting tube 220 rotates, the heat conducting tube 220 can drive the rotating cylinder 243 to rotate, the fixing rod 151 can support the fixing ring 150, and the fixing ring 150 can support the connecting ring 140. When the central tube 210 rotates, the connecting ring 140 can rotate in the fixing ring 150 through the connecting tube 211 and the heat conducting tube 220, so that the connecting gear 221 can roll in the gear ring 152, so that the connecting gear 221 can rotate, and the connecting gear 221 can drive the heat conducting tube 220 to rotate. When the heat conducting tube 220 rotates, the rotating cylinder 243 can be driven to rotate, and the rotating cylinder 243 can drive the toggle plate 244 to rotate in the conveying frame 240, and the toggle plate 2 When the conveying frame 240 is rotated, the concentrated liquid in the conveying frame 240 can be pushed out of the conveying frame 240 from the strip groove 246, and the concentrated liquid will flow between the connecting cylinder 230 and the conveying frame 240, and then flow out from the top of the connecting cylinder 230. At the same time, the concentrated liquid at the bottom of the connecting cylinder 230 can be sucked into the conveying frame 240 from the connecting groove 245, so that the concentrated liquid can quickly flow through the surface of the heat-conducting pipe 220. Since the positioning cylinder 250 and the sliding cylinder 251 can cover the heat-conducting pipe 220, the concentrated liquid can be heated only by the surface of the heat-conducting pipe 220 in the connecting cylinder 230, and the time for the concentrated liquid to flow through the surface of the heat-conducting pipe 220 is short, so that the temperature of the heating medium can be set higher, thereby quickly heating the concentrated liquid and avoiding excessive heating of the concentrated liquid.

[0093] like Fig.12 As shown, in this embodiment, two round rods 242 are fixedly connected to the top surface of the partition 241, the top ends of the two round rods 242 at the bottom are fixedly connected to the bottom surfaces of the corresponding partitions 241, the two round rods 242 at the bottom can connect the two partitions 241, and the two round rods 242 at the top can connect the positioning ring 261 to the top partition 241, and a screw-on ring 260 is arranged on the top of the heat pipe 220, and the top of the side of the screw-on ring 260 is rotatably connected to the positioning ring 261 fixedly connected to the top ends of the two adjacent round rods 242, and the top of the side of the positioning tube 250 at the top is provided with a thread 252 screwed together with the inner side of the screw-on ring 260.

[0094] During specific implementation, the top positioning cylinder 250 can limit the position of the screw-in ring 260 through the thread 252, and the screw-in ring 260 can limit the position of the top partition 241 through the positioning ring 261 and the round rod 242, thereby limiting the position of the two partitions 241, and further limiting the height of the conveying frame 240. The screw-in ring 260 can be rotated as needed to adjust the height of the screw-in ring 260. The screw-in ring 260 can drive the round rod 242 to move through the positioning ring 261, thereby moving the partition 241 and the conveying frame 240, and further adjusting the height of the conveying frame 240, so that the sliding cylinder 251 can be retracted into or extended from the positioning cylinder 250. In this way, the length of the area where the heat pipe 220 contacts the concentrated liquid can be adjusted as needed.

[0095] like Figure 8-10 As shown, in this embodiment, the unloading mechanism 160 includes a guide frame 161, two limit frames 162, two fixed seats 163, a push plate 164, and two inclined panels 165;

[0096] The two limit frames 162 are respectively arranged on the inner and outer sides of the guide frame 161, the two fixed seats 163 are fixedly connected to the bottom surfaces of the guide frame 161 and the limit frame 162, the side surfaces of the fixed seats 163 are fixedly connected to the inner side surfaces of the mixing drum 110, the fixed seats 163 can support and position the guide frame 161 and the limit frame 162, the push plate 164 is arranged on the top surface of the guide frame 161, the side surfaces of the push plate 164 are fixedly connected to the side surfaces of the push ring 141, the two inclined panels 165 are fixedly connected to the bottom surfaces of the push plate 164, and the inclined panels 165 are located between the guide frame 161 and the corresponding limit frames 162.

[0097] In a specific implementation, the powdered activated carbon and diatomaceous earth can be transported to the guide frame 161 and the limit frame 162, and the powdered activated carbon and diatomaceous earth can slide down the inclined surface of the guide frame 161 and the limit frame 162 to between the guide frame 161 and the limit frame 162. When the central tube 210 drives the connecting ring 140 to rotate, the connecting ring 140 can drive the push plate 164 and the inclined plate 165 to rotate through the push ring 141, and the push plate 164 can push the powdered activated carbon and diatomaceous earth flat, and the inclined plate 165 can move between the guide frame 161 and the limit frame 162. The powdered activated carbon and diatomaceous earth are pushed out from between the guide frame 161 and the limit frame 162 through the inclined surface of the inclined panel 165, and the bottoms of the guide frame 161 and the limit frame 162 can contact the concentrated liquid. In this way, during the falling process of the powdered activated carbon and diatomaceous earth, the powdered activated carbon and diatomaceous earth can be always blocked by the guide frame 161 and the limit frame 162 to avoid the generation of dust, and the gap between the guide frame 161 and the limit frame 162 is relatively small, so as to avoid agglomeration caused by too much falling at one time, which is conducive to the rapid dissolution of the powdered activated carbon and diatomaceous earth in the concentrated liquid.

[0098] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0099] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A preparation process of a hippocampal peptide beverage, characterized in that: The method comprises the following steps: preparing hippocampal peptide, preparing ginseng extract, mixing and stirring raw materials, homogenizing, filtering and cooling. The specific steps are as follows: Step (i) Preparation of hippocampal peptide: preparing hippocampal peptide powder using artificially cultured frozen American hippocampus linearis; Step (ii) preparing ginseng extract: preparing ginseng extract powder using ginseng; Step (iii) mixing and stirring the raw materials: according to the following ingredients per 100 grams of beverage: 1-3 g of hippocampal peptide powder, 0.7-1.5 g of ginseng extract powder, 0.3-0.5 g of functional trace elements (bio-selenium, zinc lactate, calcium lactate), 0.16-0.20 g of vitamin C, 1.2-1.7 g of flavored whey protein, 0.05 g of β-cyclodextrin, 0.05 g of sodium carboxymethyl cellulose, 0.01 g of pectin, and the rest being distilled water, after the raw materials are filled in order according to the proportion, they are put into a stirring structure for mixing and stirring; Step (iv) homogenization: homogenizing the mixed solution using a homogenizer; Step (V) Filtration: Filter the mixed solution using a sterile filter; Step (six) Cooling: Place the filtered mixed solution in a refrigerator and allow to cool to 4 degrees Celsius.

2. The preparation process of the hippocampal peptide beverage according to claim 1, characterized in that: In step (iii), the flavored whey protein is selected from strawberry whey powder, blueberry whey powder, and oolong tea water-soluble powder to prepare different flavors.

3. The preparation process of the hippocampal peptide beverage according to claim 1, characterized in that: The specific preparation steps of hippocampal peptide in step (a) include preparation of defatted hippocampal powder - ultrasonic-low temperature vacuum cooking treatment - enzymatic hydrolysis - centrifugal separation - decolorization - ultrafiltration - desalting - drying, and the specific steps are as follows: Step (1) Preparation of defatted hippocampus powder: freeze-dry frozen American hippocampus at -40°C for 48 hours, crush it with a crusher, and then remove the fatty acids of the hippocampus with supercritical fluid extraction of carbon dioxide to obtain defatted hippocampus powder; Step (2) Ultrasonic-low-temperature vacuum cooking treatment: dissolving the defatted seahorse powder obtained above in distilled water at a weight ratio of 1:15 between the material and the liquid, stirring magnetically for 30 minutes, and then performing ultrasonic treatment. The seahorse powder solution is sealed and subjected to low-temperature vacuum cooking; Step (3) Enzymatic hydrolysis: add papain and alkaline protease in an amount of 5% of defatted seahorse powder to the above solution, respectively, at pH 9.0, at a hydrolysis temperature of 50° C., for 6 h, then inactivate the enzyme at 100° C. for 15 min, and then cool to room temperature after inactivation; Step (4) centrifugation: centrifuging the above enzymatic hydrolysate at 9000 rpm for 20 min, and concentrating the obtained supernatant to 1 / 5 of the original volume to obtain a hippocampal peptide concentrate; Step (5) decolorization: the hippocampal peptide concentrate obtained in step (4) is added into a constant temperature stirring device, and then activated carbon and diatomaceous earth are added, and the mixture is stirred at 70° C. for 20 minutes by the constant temperature stirring device for decolorization; Step (6) ultrafiltration: ultrafiltration of the decolorized solution using a 20 kDa ultrafiltration membrane to obtain two hippocampal peptides with different molecular weight ranges: PH-I (molecular weight greater than 20 kDa) and PH-II (molecular weight less than 20 kDa); Step (7) Desalination: Desalting PH-II using membrane separation technology; Step (8) Drying: The desalted peptide solution is spray dried to obtain the target hippocampal peptide powder (molecular weight less than 20 kDa).

4. The preparation process of the hippocampal peptide beverage according to claim 3, characterized in that: The ultrasonic treatment time in step (2) is 20 min, the ultrasonic power is 600 W, the ultrasonic temperature is 50° C., the cooking temperature is 55° C., the cooking time is 20 min, and the vacuum degree is 0.05 MPa.

5. The preparation process of the hippocampal peptide beverage according to claim 3, characterized in that: The constant temperature stirring device in step (5) comprises: A containing module (100) is used to place the hippocampal peptide concentrate, the containing module (100) comprises a stirring drum (110), a plurality of supporting columns (111) are fixedly connected to the bottom surface of the stirring drum (110), and a material discharge mechanism (160) is arranged on the top of the inner side surface of the stirring drum (110); The stirring module (200) is arranged inside the stirring drum (110). The stirring module (200) comprises a central tube (210). The bottom of the side of the central tube (210) is rotatably connected to the inner side of the bottom of the stirring drum (110). The top and bottom of the side of the central tube (210) are both connected to two connecting tubes (211). Heat conducting tubes (220) are arranged on both sides of the central tube (210) corresponding to the connecting tubes (211). The top and bottom of the inner side of the heat conducting tube (220) are both rotatably connected to the side of the adjacent connecting tube (211). The side of the heat conducting tube (220) is rotatably connected to two connecting tubes (230). The bottom of the side of the connecting tube (230) is provided with an open groove (232). The side of the connecting tube (230) corresponding to the open groove (232) is fixedly connected to a guide plate (231).

6. The preparation process of the hippocampal peptide beverage according to claim 5, characterized in that: The containing module (100) further comprises: A drive box (120) is fixedly connected to the bottom surface of the mixing drum (110); the bottom of the side surface of the central tube (210) is rotatably connected to the inner side surface of the drive box (120); a drive motor (122) is fixedly connected to the inner bottom surface of the drive box (120); the output end of the drive motor (122) is drivingly connected to a conveying shaft (123) rotatably connected to the inner top surface of the drive box (120); two mutually meshing transmission gears (124) are arranged inside the drive box (120); the side surface of the conveying shaft (123) is fixedly sleeved with one transmission gear (124); and the bottom of the side surface of the central tube (210) is fixedly sleeved with the other transmission gear (124); and the bottom surface of the drive box (120) is fixedly connected to a conveying pipe (121) rotatably connected to the bottom end of the central tube (210); The connecting pipe (130) is rotatably connected to the top of the central pipe (210), and the bottom end of the connecting pipe (130) is fixedly connected to a fixing frame (131) fixedly connected to the inner side surface of the mixing drum (110), and the inner side surface of the fixing frame (131) is rotatably connected to the top of the central pipe (210).

7. The preparation process of the hippocampal peptide beverage according to claim 5, characterized in that: A conveying frame (240) is arranged at the top of the inner side of the connecting cylinder (230); a partition (241) slidably connected to the inner side of the connecting cylinder (230) is fixedly connected to the bottom of the side of the conveying frame (240); a rotating cylinder (243) slidably connected to the side of the heat conducting pipe (220) is rotatably connected to the inner side of the conveying frame (240); a plurality of toggle plates (244) are fixedly connected to the side of the rotating cylinder (243); a plurality of connecting grooves (245) are provided on the bottom surface of the conveying frame (240); a strip groove (246) is provided on the side of the conveying frame (240) away from the opening groove (232); a positioning cylinder (250) is fixedly connected to the top of the inner side of the connecting cylinder (230); the top surface of the positioning cylinder (250) located at the bottom is fixedly connected to the bottom surface of the corresponding connecting cylinder (230); a sliding cylinder (251) is slidably connected to the bottom of the inner side of the positioning cylinder (250); the bottom surface of the sliding cylinder (251) is fixedly connected to the top surface of the conveying frame (240).

8. The preparation process of the hippocampal peptide beverage according to claim 7, characterized in that: Two round rods (242) are fixedly connected to the top surface of the partition (241); the top ends of the two round rods (242) located at the bottom are fixedly connected to the bottom surface of the corresponding partition (241); a screw-on ring (260) is provided at the top of the heat conducting pipe (220); a positioning ring (261) fixedly connected to the top ends of the two adjacent round rods (242) is rotatably connected to the top of the side of the screw-on ring (260); and a thread (252) screwed to the inner side of the screw-on ring (260) is provided at the top of the side of the positioning cylinder (250) located at the top.

9. The preparation process of the hippocampal peptide beverage according to claim 7, characterized in that: A connecting gear (221) is fixedly sleeved on the top of the side of the heat conducting pipe (220); The containing module (100) further comprises: A connecting ring (140) is arranged at the top of the inner side of the mixing drum (110); the inner side surface of the connecting ring (140) is rotatably connected to the side surfaces of the two heat conducting tubes (220); the bottom surface of the connecting ring (140) is fixedly connected to the top surfaces of two adjacent positioning drums (250); the connecting gear (221) is located inside the connecting ring (140); and the bottom surface of the connecting ring (140) is fixedly connected to a pushing ring (141); The fixing ring (150) is rotatably connected to the inner side surface of the connecting ring (140); a plurality of fixing rods (151) fixedly connected to the inner side surface of the mixing drum (110) are fixedly connected to the side surface of the fixing ring (150); and a gear ring (152) meshing with two connecting gears (221) is provided on the inner side surface of the fixing ring (150).

10. The preparation process of the hippocampal peptide beverage according to claim 9, characterized in that: The material unloading mechanism (160) comprises: Guide frame (161); Two limit frames (162), respectively arranged on the inner side and the outer side of the guide frame (161); Two fixed seats (163) are fixedly connected to the bottom surfaces of the guide frame (161) and the limit frame (162), and the side surfaces of the fixed seats (163) are fixedly connected to the inner side surfaces of the mixing drum (110); A push plate (164) is arranged on the top surface of the guide frame (161), and a side surface of the push plate (164) is fixedly connected to a side surface of the push ring (141); The two inclined panels (165) are both fixedly connected to the bottom surface of the push plate (164), and the inclined panels (165) are located between the guide frame (161) and the corresponding limit frame (162).

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