Oral care composition capable of resisting oral biofilm as well as preparation method and application of oral care composition

Abstract

The invention relates to the technical field of oral care products, in particular to an oral care composition capable of resisting an oral biofilm as well as a preparation method and application of the oral care composition. The oral care composition for resisting oral biofilm comprises domiphen and sophorolipid. The oral care composition can be applied to preparation of oral care products including toothpaste, mouthwash, tooth powder or tooth gel. When domiphen and sophorolipid are compounded and used according to a certain proportion, in the formula of the oral care product containing the anion surfactant, the bacteriostatic action of domiphen is ensured, meanwhile, the inhibition on an acidic oral biofilm is enhanced, and the bitter taste of domiphen is also reduced. According to the technical scheme, the technical problem that the antibacterial performance is limited when domiphen is applied to the oral care product can be solved, and the domiphen oral care product has good application prospects and market popularization value.

Description

Technical Field

[0001] The invention relates to the technical field of oral care products, and in particular to an oral care composition for resisting oral biofilm, and a preparation method and application thereof. Background Art

[0002] Dental caries is a common oral disease that causes progressive damage to the color, shape, and texture of teeth, seriously affecting oral functions such as pronunciation, chewing, and language. Whether infants, adolescents, or adults, everyone is at risk of developing dental caries. The World Health Organization has listed it as one of the three major preventable diseases for humans, along with tumors and cardiovascular diseases.

[0003] Biofilm is a membrane structure formed by the adhesion and concentration of most bacteria on solid surfaces. Biofilm has strong resistance to the host defense system, low sensitivity to antibiotics, and much higher pathogenicity to humans than planktonic bacteria. The oral cavity is one of the four major bacterial reservoirs in the human body. There are many types of bacteria in the oral cavity and the number is high. Most of these bacteria adhere to the surface or inside of teeth, gums, and oral mucosa in the form of biofilms, which were often called dental plaque in the past. Biofilm is a structural microbial community formed by microorganisms attached to the surface of solid carriers and their own secreted extracellular polymers (EPS) (Costerton et al., 1999). The formation of harmful biofilms can bring about many adverse effects. Oral Streptococcus, especially Streptococcus mutans, is the main caries-related bacteria, and dental plaque biofilm is the main form of bacteria in the oral cavity. It is now known that the formation of microbial film has an adverse antagonistic effect on the inhibition and elimination of microorganisms. Compared with the MIC (minimum inhibitory concentration) of antibacterial agents for free bacteria, the production of microbial film can increase the inhibitory concentration by hundreds or thousands of times. How to efficiently and effectively inhibit bacteria under microbial films is the subject of current technology improvement and research. Epidemiological studies have found that the detection rate of Streptococcus mutans in dental plaque biofilms is positively correlated with the occurrence of dental caries. The ability of Streptococcus mutans to colonize on the tooth surface and form plaque biofilms is closely related to the etiology and pathogenesis of dental caries. The most effective way to control dental caries is plaque control, including inhibiting the formation of plaque biofilms and regularly removing plaque biofilms on the tooth surface.

[0004] There are many kinds of antibacterial agents circulating in the market, but most of them contain toxic chemical reagents, such as hypochlorous acid, peracetic acid, glutaraldehyde, etc., which pose certain safety hazards when used. In this context, natural, safe and broad-spectrum antibacterial agents are urgently needed in the market. Each antibacterial agent has different antibacterial effects on different types of bacteria. For example, hydrophilic antibacterial agents have a higher antibacterial efficiency against Escherichia coli, and lipophilic antibacterial agents have a higher antibacterial rate against Staphylococcus aureus. Domiphene is a cationic surfactant broad-spectrum bactericide with significant antibacterial properties. It mainly produces antibacterial effects by adsorbing on the surface of bacteria, changing the permeability of bacterial cytoplasmic membranes, and disrupting their metabolism. In different pH environments, the antibacterial effect of domiphene is different. In the oral cavity, especially when acidic bacteria such as Streptococcus mutans and Lactobacillus are the dominant flora, the oral cavity is an acidic environment, which greatly reduces the antibacterial effect of domiphene. The bitter taste of domiphene is one of its significant characteristics. Domiphene is a white or slightly yellow flaky crystal, odorless or with a slight odor, bitter and with a certain soapy taste. The higher the dosage of domiphene, the stronger its bitterness. In order to meet its antibacterial performance, a higher dosage of antibacterial agent needs to be added, but the increase in bitterness becomes a contradiction or restriction. Therefore, solving the reduction of domiphene's antibacterial performance under acidic conditions and solving the bitterness are challenges for it in the oral cavity. Summary of the invention

[0005] The present invention is intended to provide an oral care composition against oral biofilm, so as to solve the technical problem that the antibacterial performance of domiphene is limited when it is used in oral care products.

[0006] In order to achieve the above object, the present invention adopts the following technical scheme:

[0007] An oral care composition for resisting oral biofilm comprises domiphene and sophorolipids.

[0008] Furthermore, the mass ratio of domiphene to sophorolipid is 0.001-0.1:0.1-10.

[0009] Furthermore, the domiphene is provided in the form of a monomer, in the form of powder or powder crystals, wherein the active substance content is ≥ 97%.

[0010] Furthermore, the content of active substances in the sophorolipid is greater than 50%, and the ratio of the lactone type to the acidic type of sophorolipid is greater than 1.

[0011] The technical solution also provides an application of an oral care composition against oral biofilm in the preparation of oral care products.

[0012] Furthermore, the addition amounts of domiphene and sophorolipid in the oral care product are 0.001-0.1% and 0.1-10%, respectively.

[0013] Furthermore, the oral care product is toothpaste, mouthwash, tooth powder or tooth gel.

[0014] Furthermore, the oral care product is a toothpaste; the toothpaste comprises the following raw materials in parts by weight: 0.3-10 parts of an oral care composition for resisting oral biofilm, 10-70 parts of a moisturizer, 0-60 parts of an abrasive, 0.1-10 parts of a thickener, 0.1-20 parts of a surfactant, 0.1-2 parts of a sweetener, 0.1-5 parts of an essence, and the balance is water.

[0015] Furthermore, the moisturizing agent includes at least one of glycerin, sorbitol, polyethylene glycol, and propylene glycol.

[0016] The friction agent includes at least one of silicon dioxide, calcium bicarbonate, and calcium hydrogen phosphate dihydrate.

[0017] The thickener includes at least one of sodium carboxymethyl cellulose, hydroxyethyl cellulose, carbomer, carrageenan, guar gum and xanthan gum.

[0018] The surfactant includes at least one of sodium lauryl sulfate, ammonium cocoyl alanine triacetate, cocamidopropyl betaine, potassium cocoyl glycinate, sodium cocoyl glycinate, sodium lauroyl sarcosinate, alkyl glucoside, sodium laureth sulfate and triethanolamine cocoyl glutamate.

[0019] The sweetener includes at least one of saccharin sodium, aspartame, stevioside, sucralose, acesulfame potassium, sucrose and xylitol.

[0020] The flavor includes at least one of lemon flavor, tea flavor, spearmint flavor, wintergreen flavor, peppermint, and peppermint essential oil.

[0021] The present technical solution also provides a method for preparing an oral care product containing an oral care composition that resists oral biofilm, comprising the following steps performed in sequence:

[0022] S1: After mixing the oral care composition, ultrasonically disperse it for 10-15 minutes to obtain a mixture A;

[0023] S2: adding a binder to a humectant, stirring, adding a sweetener, a surfactant and water, and mixing to obtain a mixture B;

[0024] S3: Mixing mixture A and mixture B, adding a friction agent, stirring and vacuuming;

[0025] S4: adding essence, stirring and homogenizing, and degassing to obtain a toothpaste product.

[0026] In summary, the principle of this technical solution is:

[0027] This technical solution uses the antibacterial substance domiphene. Domiphene has the best effect in neutral and weakly alkaline solutions. Its effectiveness decreases in the presence of acidic organic substances and pus and blood. It is generally incompatible with anionic surfactants due to charge neutralization. Domiphene has a certain inhibitory effect on free oral bacteria, but when used in oral care products such as toothpaste, the anionic surfactants commonly used in the formula reduce the antibacterial ability due to the potential neutralization effect; in addition, when the microorganisms in the mouth form a microbial film, the local acidity increases, which also causes the antibacterial effect of domiphene to decrease. These are technical difficulties that need to be solved to prevent the ingredient from taking effect. The inventor unexpectedly found in the study that when domiphene and sophorolipids are used in a certain proportion, the antibacterial effect of domiphene is not reduced in the formula of oral care products containing anionic surfactants, and the inhibitory effect of acidic oral biofilms is synergistic. When used in combination with other sugar ester surfactants, there is no above-mentioned synergistic effect, and the antibacterial ability decreases. Not only that, the inventors also found that further research revealed that the combination of domiphene and sophoroside esters also reduced the bitterness of domiphene, further optimizing the product.

[0028] In addition, the inventor prepared the above composition into an oral care product and further studied its preparation method. It was found that the preparation method has a significant impact on the stability of the product. The composition addition method using the process scheme of the present invention has a positive impact on the stability of the product, meets the requirements of the stability of the long-term storage of the product, and has important breakthrough significance. DETAILED DESCRIPTION

[0029] The present invention is further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the technical means used in the following examples and experimental examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used can be obtained from commercial sources.

[0030] Example

[0031] An oral care composition for resisting oral biofilm, comprising domiphene and sophorolipids in a mass ratio of 0.001-0.1:0.1-10 (preferably 0.001-0.1:0.3-10). Domiphene (CAS: 538-71-6) is provided in the form of a monomer, which may be a powder or a powdered crystal, wherein the active substance is greater than or equal to 97%. Sophorolipids are a natural biosurfactant produced by fermentation of non-pathogenic yeasts and have an excellent taste. The sophorolipids of the present invention can be obtained by fermentation, and may be a fermentation broth extract or concentrate, or may be a pure product obtained by separation and purification with an organic solvent, or may be in the form of a liquid, semi-solid, solid paste or powder. Sophorolipids are divided into lactone type and acid type. The present invention adopts a mixed type of lactone type and acid type, with an active substance content of >50%, and a ratio of lactone type to acid type >1.

[0032] The above-mentioned oral care composition against oral biofilm can be used in the preparation of oral care products, and the oral care products can be toothpaste, mouthwash, tooth powder or tooth gel. The addition amounts of domiphene and sophorolipid in the oral care products are 0.001-0.1% and 0.1-10% (preferably 0.3-10%) respectively.

[0033] Taking toothpaste as an example, its raw materials include, by weight: 0.301-10.1 parts of an oral care composition for resisting oral biofilm (including 0.001-0.1 parts of domiphene and 0.3-10 parts of sophorolipids), 10-70 parts of a moisturizer, 0-60 parts of an abrasive, 0.1-10 parts of a thickener, 0.1-20 parts of a surfactant, 0.1-2 parts of a sweetener, 0.1-5 parts of a flavor, 0.01-0.5 parts of a pH adjuster, and the remainder is water, a total of 100 parts.

[0034] The humectant includes at least one of glycerin, sorbitol, polyethylene glycol and propylene glycol.

[0035] The friction agent includes at least one of silicon dioxide, calcium bicarbonate, and calcium hydrogen phosphate dihydrate.

[0036] The thickener includes at least one of sodium carboxymethyl cellulose, hydroxyethyl cellulose, carbomer, carrageenan, guar gum and xanthan gum.

[0037] The surfactant includes at least one of sodium lauryl sulfate (sodium lauryl sulfate), cocoyl alanine triacetate ammonium, cocamidopropyl betaine, potassium cocoyl glycinate, sodium cocoyl glycinate, sodium lauroyl sarcosinate, alkyl glucoside, sodium laureth sulfate and triethanolamine cocoyl glutamate.

[0038] The sweetener includes at least one of saccharin sodium, aspartame, stevioside, sucralose, acesulfame potassium, sucrose and xylitol.

[0039] The flavor includes at least one of lemon flavor, tea flavor, spearmint flavor, wintergreen flavor, peppermint, and peppermint essential oil.

[0040] The preparation method of the toothpaste containing the oral care composition for resisting oral biofilm comprises the following steps:

[0041] Step 1: After mixing the oral care composition (domiphene and sophorolipid), disperse it by ultrasound (frequency 19-21 Khz, power 500-2000 W) for 10-15 minutes to obtain a mixture A.

[0042] Step 2: Add a thickener to the moisturizer, stir, add a sweetener, a surfactant and water, and mix to obtain a mixture B.

[0043] Step 3: Mix mixture A and mixture B, add abrasive, stir and vacuum.

[0044] Step 4: adding essence, stirring and homogenizing, and degassing to obtain a toothpaste product.

[0045] Experimental Example 1: Evaluation of biofilm removal effect

[0046] (1) Cultivation of biofilm model

[0047] The overnight resuscitation culture of Streptococcus mutans ATCC (ATCC 700610 third generation fresh culture) and Fusobacterium nucleatum (ATCC 25586 third generation fresh culture) were diluted to 10 with BHIS. 6 cfu / mL, and then added to a 24-well plate with sterile hydroxyapatite sheets (10×1.2 mm), 2 mL of bacterial solution per well, and cultured anaerobically at 37°C for 24 hours to form a biofilm.

[0048] (2) Long-term effects of simulated raw materials on biofilm

[0049] Twice a day after the biofilm is formed, remove the hydroxyapatite sheet under sterile operation and transfer it to a well plate containing 5 mL of the test solution. After 5 minutes at 37°C, transfer the hydroxyapatite sheet carrying the biofilm to a well plate containing fresh BHIS culture medium and continue to culture anaerobically at 37°C. The above operation is carried out for 5 consecutive days. After the treatment and culture on the last day, wash the biofilm on the hydroxyapatite sheet with PBS and measure the OD 550 The test solution is prepared by diluting the toothpaste sample with laboratory grade 1 water in a mass ratio of 3:1, centrifuging and filtering, and taking the supernatant.

[0050] For the biofilm removal effect of the test composition, the removal rate relative to the control group was calculated by the following formula, and the oral biofilm removal effect was determined according to the following criteria based on the removal rate. The larger the value, the better the removal effect. In addition, 5 ml of sterile distilled water was used instead of the above test solution, and the rest was treated in the same way, which was used as the control group.

[0051] Biofilm removal rate (%) = {(turbidity of control group - turbidity of test composition treatment) / turbidity of control group} × 100%

[0052] The specific composition of the solution to be tested and the biofilm removal rate are shown in Table 1. The preparation method of toothpaste is shown in the previous embodiment (wherein the frequency of ultrasonic dispersion is specifically: 20Khz, the power is specifically: 1000W, and the duration is specifically: 10 minutes). The sophorolipids in the present invention are purchased from Shaanxi Deguan Biotechnology Co., Ltd., and the content of active substances (internal fat type and acidic sophorolipids) is specifically about 85%, and the ratio of internal fat type and acidic sophorolipids is specifically about 70:30. Among them, rhamnolipids (cas: 869062-42-0) and sophorolipids are both natural surfactants of glycolipids and have certain antibacterial effects.

[0053] Table 1: Specific composition of the tested solution and biofilm removal rate (the total amount of the formula is 100, and the following experimental results are the average of three repeated experiments)

[0054]

[0055] From the above experimental results, it can be seen that in Tests 1-4, the combination of domiphene and sophorolipids has a relatively ideal biofilm removal efficiency, and the biofilm removal efficiency increases with the increase of concentration.

[0056] In comparison test 1 with test 4, only an equal amount of domiphene was added without adding sophorolipids, resulting in a significant decrease in the biofilm removal rate, which dropped sharply from 85.08% to 18.64%.

[0057] Comparative Test 2 Compared with Test 4, the same amount of sophorolipids was replaced with rhamnolipids. Although both sophorolipids and rhamnolipids have antibacterial effects and are natural surfactants of glycolipids, the combined use of rhamnolipids and domiphene is not very ideal in inhibiting biofilm. Compared with domiphene alone (Comparative Test 1), the introduction of rhamnolipids did not effectively improve the biofilm removal rate of oral care products. In Test 4, the combined use of domiphene and sophorolipids produced a great gain effect on the biofilm removal rate. This shows that there is a unique synergistic effect between sophorolipids and domiphene, and sophorolipids cannot be replaced by other similar substances.

[0058] In comparative test 3, sophorolipids were added, but domiphene was not added. Since the amount of sophorolipids added was not large, no obvious antibacterial effect was shown, and compared with comparative test 6 in which no sophorolipids and domiphene were added, no significant improvement was shown. It can be seen that in the absence of domiphene, sophorolipids are difficult to effectively inhibit biofilms.

[0059] Comparing the data of comparative test 1 (0.1 part of domiphene), comparative test 7 (10 parts of sophorolipids), and test 4 (0.1 part of domiphene + 10 parts of sophorolipids), the biofilm removal rates of each group were 18.64%, 13.58%, and 85.08%, respectively. Test 4 combines domiphene and sophorolipids, and its biofilm removal rate is higher than the sum of the biofilm removal rates of comparative test 1 and comparative test 7, indicating that the combined use of domiphene and sophorolipids has a synergistic effect in improving the biofilm removal rate. Comparing the data of comparative test 5 (0.001 part of domiphene), comparative test 8 (0.3 part of sophorolipids), and test 1 (0.001 part of domiphene + 0.3 part of sophorolipids), the biofilm removal rates of each group were 11.34%, 5.64%, and 35.78%, respectively. It can be seen that at this concentration, the combined use of domiphene and sophorolipids has a synergistic effect.

[0060] Experimental Example 2: Bitterness Sensory Test

[0061] The quantitative sensory description method was used to divide the intensity of bitterness into 10 levels from strong (very bitter, unbearable) to weak (no bitterness at all), and the bitterness intensity decreased from high to low (9 points-0 points). 15 sensory evaluators participated in the scoring of the test samples. The test samples were brushed for 1 minute and then kept in the mouth for 30 seconds. The test samples were evaluated and scored based on their feelings. In order to avoid mutual interference between test samples, the test time interval must be more than 2 hours. The bitterness of the test sample took the average score of the sensory evaluators as the final score. The test results are shown in Table 2.

[0062] Table 2: Bitterness sensory test evaluation results

[0063] Test sample (composition see Experimental Example 1) Bitterness sensory score Test 1 0.07 Test 3 1.33 Test 4 4.44 Comparison test 1 7.86 Comparison test 5 3.13 Comparison test 3 0.07

[0064] From the experimental test results in Table 2, it can be seen that the bitterness of domiphene added to oral products can be clearly felt at low levels of addition. In the high-level addition of comparison test 1, the bitterness sensory score reached 7.96, and the low-level addition also reached 3.13 (comparison test 5). This bitter feeling is often not expected or accepted in oral daily products, so the expectation for its improvement is very urgent. In test 1, test 3, and test 4, we saw a surprising improvement effect. After adding sophorolipids, it showed 4.44 in test 4 with high-level addition, 1.33 in test 3 with low level, and 0.07 in test 1 with low level, reaching the level where bitterness is imperceptible or basically imperceptible. This improvement effect is huge and expected. Comparing the addition of domiphene at the same level, such as: comparing test 1 with test 4, the bitterness test score dropped from 7.86 to 4.44, and the degree of decline was significant and effective.

[0065] Experimental Example 3: Different production steps of toothpaste and paste stability test

[0066] In this experimental example, the following comparative test contents are added as some attempts before the final process of this scheme is determined.

[0067] Comparative Test 9: The toothpaste of Test 2 was prepared as follows:

[0068] Step 1: After adding the oral care composition (domiphene and sophorolipids), mix for 15 minutes under anchor stirring at 25 rpm to obtain a mixture A.

[0069] Step 2: Add a thickener to the moisturizer, stir, add a sweetener, a surfactant and water, and mix to obtain a mixture B.

[0070] Step 3: Mix mixture A and mixture B, add abrasive, stir and vacuum.

[0071] Step 4: adding essence, stirring and homogenizing, and degassing to obtain a toothpaste product.

[0072] Comparative Test 10: The toothpaste of Test 2 was prepared as follows:

[0073] Step 1: After adding the oral care composition (domiphene and sophorolipid), ultrasonic dispersion and mixing were performed at 20Khz and 1000W for 5 minutes to obtain a mixture A.

[0074] Step 2: Add a thickener to the moisturizer, stir, add a sweetener, a surfactant and water, and mix to obtain a mixture B.

[0075] Step 3: Mix mixture A and mixture B, add abrasive, stir and vacuum.

[0076] Step 4: adding essence, stirring and homogenizing, and degassing to obtain a toothpaste product.

[0077] Comparative Test 11: The toothpaste of Test 2 was prepared as follows:

[0078] Step 1: After adding the oral care composition (domiphene and sophorolipid), ultrasonic dispersion and mixing were performed at 20Khz and 1000W for 35 minutes to obtain a mixture A.

[0079] Step 2: Add a thickener to the moisturizer, stir, add a sweetener, a surfactant and water, and mix to obtain a mixture B.

[0080] Step 3: Mix mixture A and mixture B, add abrasive, stir and vacuum.

[0081] Step 4: adding essence, stirring and homogenizing, and degassing to obtain a toothpaste product.

[0082] At room temperature and 45°C, the toothpastes obtained from Tests 1-4 and Comparative Tests 1-11 were subjected to a 90-day stability observation. The experimental results are shown in Table 3.

[0083] In the observation of paste stability, normal means: the paste is uniform without discoloration, phase separation, coarsening, etc.; slight shelling means: the paste is slightly uneven, and liquid separates from the paste, the degree is relatively light; shelling means that the paste is uneven, and liquid separates from the paste, the degree is moderate or severe. In Table 3, the " / " before the test indicates the result at room temperature, and the " / " after the test indicates the test result at 45℃; if there is no " / ", it means that the test result at room temperature is consistent with the test result at 45℃.

[0084] Table 3: Paste appearance stability test results

[0085]

[0086]

[0087] The toothpaste paste prepared in test 1-4 and comparative test 1-3, 5 has good stability. According to the observation results of comparative test 4, after the use of high concentration of domiphene in the toothpaste formula, the quality stability of the toothpaste formula is adversely affected, and the shelling phenomenon gradually occurs. The possible reason is that domiphene is a quaternary ammonium salt cationic surfactant, which contacts with the anionic surfactant sodium dodecyl sulfate in the formula, and slow potential neutralization occurs. When the dosage is too large, the surface charge of the solid and liquid is unbalanced, causing the formula to produce solid-liquid separation, liquid precipitation and other undesirable phenomena. However, the technical solution unexpectedly overcomes this adverse effect through the combination technology of domiphene and sophorolipids, and meets the requirements of commercial long-term storage stability. In addition, the inventors found that the realization of the technical combination requires process measures under certain conditions. Under the treatment of anchor stirring, which is a common process in the technical field (Comparative Test 9), the product eventually showed coarsening and slight water separation, and when the ultrasonic process time was insufficient, coarsening and slight water separation also appeared (Comparative Test 10), indicating that the process conditions cannot achieve a stable composition, and the charge imbalance flocculation phenomenon is more obvious in the long-term aging experiment. Under the long ultrasonic emulsification process conditions (Comparative Test 11), excessive stirring occurred, which caused the material to overheat and slightly carbonize, and the normal production of the product could not be achieved.

[0088] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An oral care composition for resisting oral biofilm, characterized in that: Includes domiphene and sophorolipids.

2. An oral care composition for resisting oral biofilm according to claim 1, characterized in that: The mass ratio of domiphene to sophorolipid is 0.001-0.1:0.1-10.

3. An oral care composition for resisting oral biofilm according to claim 2, characterized in that: The domiphene is provided in the form of a monomer, in the form of powder or powder crystals, wherein the active substance content is ≥ 97%.

4. The oral care composition for resisting oral biofilm according to claim 2, characterized in that: The active substance content in the sophorolipid is greater than 50%, and the ratio of the lactone type to the acidic sophorolipid is greater than 1.

5. Use of an anti-oral biofilm oral care composition according to any one of claims 1 to 4 in the preparation of oral care products.

6. Use of an anti-oral biofilm oral care composition according to claim 5 in the preparation of oral care products, characterized in that: The addition amounts of domiphene and sophorolipid in oral care products are 0.001-0.1% and 0.1-10% respectively.

7. Use of an anti-oral biofilm oral care composition according to claim 6 in the preparation of oral care products, characterized in that: The oral care product is toothpaste, mouthwash, tooth powder or tooth gel.

8. Use of an anti-oral biofilm oral care composition according to claim 7 in the preparation of oral care products, characterized in that: The oral care product is toothpaste; the toothpaste comprises the following raw materials in parts by weight: 0.3-10 parts of an oral care composition for resisting oral biofilm, 10-70 parts of a moisturizer, 0-60 parts of an abrasive, 0.1-10 parts of a thickener, 0.1-20 parts of a surfactant, 0.1-2 parts of a sweetener, 0.1-5 parts of an essence, and the balance is water.

9. Use of an anti-oral biofilm oral care composition according to claim 8 in the preparation of oral care products, characterized in that: The humectant includes at least one of glycerin, sorbitol, polyethylene glycol and propylene glycol. The friction agent includes at least one of silicon dioxide, calcium bicarbonate, and calcium hydrogen phosphate dihydrate. The thickener includes at least one of sodium carboxymethyl cellulose, hydroxyethyl cellulose, carbomer, carrageenan, guar gum and xanthan gum. The surfactant includes at least one of sodium lauryl sulfate, ammonium cocoyl alanine triacetate, cocamidopropyl betaine, potassium cocoyl glycinate, sodium cocoyl glycinate, sodium lauroyl sarcosinate, alkyl glucoside, sodium laureth sulfate and triethanolamine cocoyl glutamate. The sweetener includes at least one of saccharin sodium, aspartame, stevioside, sucralose, acesulfame potassium, sucrose and xylitol. The flavor includes at least one of lemon flavor, tea flavor, spearmint flavor, wintergreen flavor, peppermint, and peppermint essential oil.

10. A method for preparing an oral care product containing an oral care composition that resists oral biofilm, characterized in that: The method includes the following steps: S1: After mixing the oral care composition, ultrasonically disperse it for 10-15 minutes to obtain a mixture A; S2: adding a binder to a humectant, stirring, adding a sweetener, a surfactant and water, and mixing to obtain a mixture B; S3: Mixing mixture A and mixture B, adding a friction agent, stirring and vacuuming; S4: adding essence, stirring and homogenizing, and degassing to obtain a toothpaste product.

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