Oil control liquid composition with pore minimizing function and preparation method of oil control liquid composition

Abstract

The invention discloses an oil control liquid composition with a pore minimizing function and a preparation method of the oil control liquid composition, and belongs to the technical field of daily chemical products. The composition is prepared from the following components in parts by weight: 2 to 5 parts of mesenchymal stem cell extract, 10 to 15 parts of herba portulacae extract, 10 to 15 parts of rosemary extract, 5 to 10 parts of sodium hyaluronate, 5 to 10 parts of salicylic acid, 10 to 15 parts of tea tree essential oil, 10 to 15 parts of honey paste and 15 to 48 parts of sterile water. According to the invention, the biological extract is used as a main component, so that pores can be effectively astringed, the problem of excessive skin grease secretion is solved, the irritation to the skin is small, and the skin is not excessively dry or even sensitive or decrustation phenomenon is avoided; meanwhile, the mesenchymal stem cell extract in the formula contains rich growth factors and cell factors, can promote regeneration and repair of skin cells, can promote metabolism and regeneration of the skin cells, can accelerate apoptosis of coarse pores with problems, accelerates growth of new pores, and fundamentally solves the problem of coarse pores.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemicals, and particularly relates to an oil-control liquid composition with a pore-tightening function and a preparation method thereof. Background Art

[0002] Oil control products are an important category of daily chemical products. With the accelerated pace of life and increased work pressure, many people are facing skin problems caused by excessive oil secretion, such as oily skin, enlarged pores, acne, etc. Therefore, consumers are increasingly demanding products that can control oil secretion and improve skin quality. The daily chemical industry has made continuous technological innovations in the research and development of oil control products, including the use of new oil control ingredients, adjustment of formulas to improve oil control effects, and improvement of product experience to continuously launch new oil control product series to meet the needs of different consumer groups.

[0003] In order to reduce sebum secretion, many oil control products contain strong degreasing ingredients, which may cause excessive dryness of the skin, or even cause sensitivity or peeling. Many oil control products may only reduce oiliness within a short period of time after use, and have limited effect on long-term control of sebum secretion. The chemical ingredients in some oil control products may have a negative impact on the skin, such as clogging pores, causing allergies or irritation, especially for people with sensitive skin. At the same time, enlarged pores caused by excessive oil secretion are still a problem to be solved. With the advancement of science and technology, skin care brands continue to develop new ingredients that can promote skin cell regeneration and repair, thereby improving the problem of enlarged pores. However, effective improvement of enlarged pores usually requires time and continuous use. For example, in the Chinese patent with publication number CN108309843A, the astringent pore factor used in its components is too slow for the pore astringency effect, and it takes a lot of time to achieve the expected effect through continuous use. Some treatment methods may cause skin allergies or adverse reactions. For example, in the Chinese patent with publication number CN115607491A, the components contain ethanol and isopropanol, which are easy to cause allergic reactions, especially for people with sensitive skin.

[0004] In summary, although oil control products are effective in solving oily skin problems, there are still some limitations in use and potential skin problems. At the same time, the accompanying problem of enlarged pores is still a big challenge. With the advancement of technology and market competition, there is still a lot of room for development of such products.

[0005] In view of the above technical defects, a solution is now proposed. Summary of the invention

[0006] The present invention aims to provide an oil-control liquid composition with the function of tightening pores, which can effectively tighten pores and solve the problem of excessive oil secretion of the skin, and has less irritation to the skin and will not cause excessive drying of the skin, sensitivity and peeling.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] An oil-control composition with pore-tightening function is composed of the following components by weight: 2-5 parts of mesenchymal stem cell (MSC) extract, 10-15 parts of purslane extract, 10-15 parts of rosemary extract, 5-10 parts of sodium hyaluronate, 5-10 parts of salicylic acid, 10-15 parts of tea tree essential oil, 10-15 parts of honey mud and 15-48 parts of sterile water.

[0009] Furthermore, a method for preparing an oil-control liquid composition having the function of tightening pores is as follows:

[0010] A1: The preparation of mesenchymal stem cell extract includes three steps: obtaining mesenchymal stem cells, culturing mesenchymal stem cells, and collecting and freeze-drying them;

[0011] A2: Add tea tree essential oil, honey mud and salicylic acid into pure water and place in a magnetic stirrer, heat in a water bath, set the temperature to 65°C, set the speed to 1200 rpm, and stir for 30 minutes to obtain material A;

[0012] A3: Mix and dissolve the purslane extract, rosemary extract and MSC extract in purified water, add sodium hyaluronate, place the mixture on a magnetic stirrer, heat in a water bath, set the temperature to 40°C, set the speed to 600 rpm, and stir for 30 minutes to obtain material B;

[0013] A4: Cool material A and material B to room temperature, and after cooling to room temperature, mix material A and material B and place them in a magnetic therapy stirrer, set the speed to 600 rpm and stir for 2 hours to obtain an oil control liquid composition.

[0014] Furthermore, the molecular weight of the sodium hyaluronate is 6000-8000 Daltons; the purslane extract is a water-soluble purslane extract powder with a specification of 15:1; and the rosemary extract is a water-soluble rosemary extract powder with a specification of 15:1.

[0015] It is further obtained that the mesenchymal stem cell acquisition comprises the following steps:

[0016] B1: Mix ampicillin, kanamycin and streptomycin at a concentration of 1 / 1000 to prepare a triple antibody; soak healthy bovine bone marrow tissue in 75% disinfectant alcohol, wash for 15 minutes, then take out the bovine bone marrow tissue and transfer it to the triple antibody and soak for 30 minutes;

[0017] B2: After soaking, transfer the bovine bone marrow tissue to sterile water and soak and wash for 15 minutes;

[0018] B3: Place the processed bovine bone marrow tissue into 10% bovine serum DMEM culture medium, use a sterile glass slide to press the bovine bone marrow tissue to the bottom of the culture dish for normal culture, observe once a day, change the culture medium every two days, and wait for the MSC cells to crawl out from the bottom of the culture dish.

[0019] Furthermore, the mesenchymal stem cell culture comprises the following steps:

[0020] C1: Wait for a large number of MSC cells to form colonies at the bottom of the culture dish before removing the bovine bone marrow tissue;

[0021] C2: Use 9% trypsin to digest and passage MSC cells. When the MSC cells have completed passage and have grown all over the bottom of the culture dish, replace the DMEM medium with serum-free medium and culture for 24 hours.

[0022] Further, the collection and lyophilization comprises the following steps:

[0023] D1: MSC cells were digested normally and frozen in a liquid nitrogen tank;

[0024] D2: Collect serum-free medium after culture completion;

[0025] D3: Add 5% mannitol to the culture medium, the ratio of serum-free culture medium to 5% mannitol is 20:1, mix well and place in a freeze dryer for 48 hours, and collect the MSC extract freeze-dried powder.

[0026] In summary, due to the adoption of the above technical scheme, the beneficial effects of the present invention are as follows: the present invention uses biological extracts as the main ingredients to effectively tighten pores and solve the problem of excessive oil secretion of the skin, and has less irritation to the skin and will not cause excessive dryness of the skin, or even cause sensitivity or peeling; at the same time, the formula of the present invention contains rich growth factors and cytokines, which can promote the regeneration and repair of skin cells, and at the same time promote the metabolism and regeneration of skin cells, which can accelerate the apoptosis of enlarged pores with problems, accelerate the growth of new pores, and solve the problem of enlarged pores from the root. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The present invention is a flow chart of a method for preparing an oil-control liquid composition having the function of tightening pores. DETAILED DESCRIPTION

[0028] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments and diagrams.

[0029] The experimental methods in the following examples, unless otherwise specified, are conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials and reagents used in the following examples, unless otherwise specified, can be obtained from commercial channels.

[0030] Example 1

[0031] 1. Mix 2 ml of ampicillin, 2 ml of kanamycin and 2 ml of streptomycin at a concentration of 1 / 1000 to make a triple antibody and dissolve it in 30 ml of 75% disinfectant alcohol.

[0032] 2. Take healthy bovine bone marrow tissue and soak it in 50ml 75% disinfectant alcohol. After washing for 15 minutes, take out the bovine bone marrow tissue and transfer it to the third antibody and soak it for 30 minutes.

[0033] 3. After soaking, transfer the bovine bone marrow tissue to 50 ml of sterile water and soak and wash for 15 minutes.

[0034] 4. Place the processed bovine bone marrow tissue in a culture dish, add 15 ml of 10% fetal bovine serum DMEM culture medium, use a sterile glass slide to press the bovine bone marrow tissue to the bottom of the culture dish for normal culture, observe once a day, change the culture medium every two days, and wait for MSC to crawl out from the bottom of the culture dish.

[0035] 5. On the 16th day, when a large number of MSC clones were present at the bottom of the culture dish, the bovine bone marrow tissue was removed and the MSCs were passaged using 9% Tris-EDTA trypsin. When the passaged MSCs covered the bottom of the culture dish, the DMEM medium was replaced with serum-free medium in equal proportion and cultured for 24 hours.

[0036] 6. The MSCs were digested and frozen normally in a liquid nitrogen tank, and the serum-free culture medium after culture was collected. 5% mannitol was added to the culture medium, mixed well, and freeze-dried in a freeze dryer for 48 hours.

[0037] The ratio of serum-free medium to 5% mannitol in the freeze-drying step is 20:1.

[0038] Finally, after 48 hours of freeze-drying, a total of 30 g of MSC extract was obtained.

[0039] Table 1, reagent parameters used in Example 1

[0040] Drug Name source model parameter Ampicillin BBI A430258 200mg Kanamycin BBI A430277 200mg Streptomycin BBI A430977 500mg Fetal bovine serum BBI E510002 500ml DMEM BBI E600008 500ml Trypsin Diamond A100458 50g Mannitol BBI A430632 100mg

[0041] Example 2

[0042] 1. Add 15 ml of tea tree essential oil, 15 g of honey mud and 10 g of salicylic acid to 15 ml of sterile water and place it on a magnetic stirrer. Heat it in a water bath, set the temperature to 65°C, set the speed to 1200 rpm, and stir for 30 minutes to obtain material A.

[0043] 2.15g of purslane extract, 15g of rosemary extract and 5g of MSC extract were mixed and dissolved in pure water, 10g of sodium hyaluronate was added, the mixture was placed on a magnetic stirrer, heated in a water bath, the temperature was set to 40°C, the speed was set to 600rpm, and stirred for 30min to obtain material B.

[0044] 3. Cool material A and material B to room temperature. After cooling to room temperature, mix material A and material B and place them in a magnetic therapy mixer. Set the speed to 600 rpm and stir for 2 hours.

[0045] 4. After stirring, 100 ml of an oil-control liquid composition having the function of tightening pores is obtained.

[0046] Table 2, reagent parameters used in Example 2

[0047] Drug Name source model parameter Tea Tree Oil Guangzhou Jiaqi Biotechnology Co., Ltd. HC011 1kg Honey Mud Mengcheng County Beekeeping Professional Cooperative YHM-01 75kg Salicylic acid Guangzhou Qiyuan Biotechnology Co., Ltd. BIOGENIC 100g Purslane Extract Xi'an Jinheng Chemical Co., Ltd. JH01 10:1 Rosemary Extract Xi'an Dongchi Biotechnology Co., Ltd. DC21022403 10:1 Sodium Hyaluronate Xi'an Zhiyuan Biotechnology Co., Ltd. 9067-32-7 1kg

[0048] Example 3

[0049] 1. Add 10 ml of tea tree essential oil, 10 g of honey mud and 5 g of salicylic acid to 48 ml of sterile water and place it on a magnetic stirrer. Heat it in a water bath, set the temperature to 65°C, set the speed to 1200 rpm, and stir for 30 min to obtain material A.

[0050] 2.10g of purslane extract, 10g of rosemary extract and 2g of MSC extract were mixed and dissolved in pure water, 5g of sodium hyaluronate was added, and the mixture was placed on a magnetic stirrer, heated in a water bath, the temperature was set to 40°C, the speed was set to 600rpm, and stirred for 30min to obtain material B.

[0051] 3. Cool material A and material B to room temperature. After cooling to room temperature, mix material A and material B and place them in a magnetic therapy mixer. Set the speed to 600 rpm and stir for 2 hours.

[0052] 4. After stirring, 100 ml of an oil-control liquid composition having the function of tightening pores is obtained.

[0053] Table 3, reagent parameters used in Example 3

[0054] Drug Name source model parameter Tea Tree Oil Guangzhou Jiaqi Biotechnology Co., Ltd. HC011 1kg Honey Mud Mengcheng County Beekeeping Professional Cooperative YHM-01 75kg Salicylic acid Guangzhou Qiyuan Biotechnology Co., Ltd. BIOGENIC 100g Purslane Extract Xi'an Jinheng Chemical Co., Ltd. JH01 10:1 Rosemary Extract Xi'an Dongchi Biotechnology Co., Ltd. DC21022403 10:1 Sodium Hyaluronate Xi'an Zhiyuan Biotechnology Co., Ltd. 9067-32-7 1kg

[0055] Comparative Example 1

[0056] Example 1 of Chinese patent publication number CN108309843A was used as comparative example 1.

[0057] Comparative Example 2

[0058] As Comparative Example 2, Example 1 of Chinese Patent Publication No. CN115607491A was used.

[0059] Cytotoxicity assay:

[0060] Example 2, Example 3, Comparative Example 1 and Comparative Example 2 were diluted to 1% and 10% respectively using sterile water to prepare concentration gradients for cytotoxicity testing.

[0061] 1. A cytotoxicity experiment was performed using 293T cells and an MTT kit with a catalog number of M1020 produced by Solarbio to detect the toxic effects of the oil control liquid composition prepared in Examples 2 and 3 on cells.

[0062] 2. The MTT kit used contains MTT solution and Formazan solution.

[0063] 3. Resuscitate and culture 293T cells. Collect logarithmic phase 293T cells one day later, adjust the concentration of cell suspension, and distribute them in 96-well plates, 180ul per well, 3000-10000 cells / well.

[0064] 4. Place the 96-well plate in a constant temperature incubator at 37°C and 5% CO2 for 6 hours.

[0065] 5. Add 10ul of the test sample to each well and set up 3 groups for each gradient.

[0066] 6. After aspirating the supernatant, add 90ul of fresh culture medium, then add 10ul of MTT solution and continue culturing for 4h.

[0067] 7. Then aspirate the supernatant, add 110ul Formazan solution to each well, place on a shaker and shake for 10 minutes to fully dissolve the crystals. After shaking, transfer the 96-well plate to an ELISA reader and measure the absorbance of each well at 490nm.

[0068] 8. Set up blank wells (culture medium, MTT solution, Formazan solution) and control wells (293T cells, sterile water, culture medium, MTT solution, Formazan solution) at the same time, with 3 replicates per well.

[0069] Table 4, MTT cytotoxicity test results

[0070] Grouping Cytotoxicity Cell viability 1% Example 2 Non-toxic / 1% Example 3 Non-toxic / 1%Comparative Example 1 Non-toxic / 1%Comparative Example 2 Non-toxic / 10% Example 2 0.47％ 99.53％ 10% Example 3 0.17％ 99.83％ 10% Comparative Example 1 1.36％ 98.63％ 10% Comparative Example 2 2.11％ 97.89％ 100% Example 2 17.23％ 82.77％ 100% Example 3 13.15％ 86.85％ 100% Comparative Example 1 27.27％ 73.73％ 100% Comparative Example 2 21.45％ 78.55％

[0071] It can be seen from Table 1 that the cytotoxicity of Example 2 and Example 3 is basically negligible when the dilution ratio is 10%, and there is no cytotoxicity in Example 2 and Example 3 when the dilution ratio is 1%.

[0072] Efficacy evaluation:

[0073] Experimental samples: Examples 2 and 3 and Comparative Examples 1 and 2 were added into the skin care lotion external base to prepare skin care lotion, wherein the oil control liquid composition with pore tightening function was added in an amount of 1 wt %. The control group used the skin care lotion external base.

[0074] 50 subjects aged 22-45 were selected, all of whom had oily and mixed skin without allergy history, and were divided into 5 groups, 5 people in each group. The subjects used the test sample once in the morning and evening for 8 weeks. The forehead was tested 3 times with a skin oil test probe (Sebumeter, German CK) before use and after 8 weeks of use, and the average value was taken to evaluate the oil control effect of the composition of the present invention. The skin pore diameter was tested using a skin image analyzer, and the nose was selected as the test site, and the measurement was performed before use and after 8 weeks of use. The pores at the same position were selected for video recording, and the pore diameter size was analyzed to evaluate the effect of the composition of the present invention with the effect of purifying pores on shrinking pores.

[0075] Table 5, Evaluation of oil control efficacy

[0076] Grouping Before use After 8 weeks of use Grease change rate Example 2 Group 112.12±29.07 78.57±22.15 -29.92％ Example 3 Group 118.34±30.17 75.43±19.16 -36.26％ Comparative Example 1 116.47±31.22 86.76±23.17 -25.51％ Comparative Example 2 114.36±29.43 92.38±21.17 -19.22％ Control group 119.66±30.85 117.48±29.65 -1.82％

[0077] Table 6, Changes in skin pore diameter before and after use

[0078]

[0079]

[0080] From the analysis of Table 5 and Table 6, it can be seen that the oil-control liquid composition with pore-tightening function prepared by the present invention has obvious oil-control and pore-tightening effects when added to skin care lotion.

[0081] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. An oil-control liquid composition having the function of tightening pores, characterized in that: The invention is composed of the following components by weight: 10-15 parts of purslane extract, 10-15 parts of rosemary extract, 5-10 parts of sodium hyaluronate, 5-10 parts of salicylic acid, 10-15 parts of tea tree essential oil, 10-15 parts of honey mud, 15-48 parts of sterile water and 2-5 parts of mesenchymal stem cell extract; The mesenchymal stem cell extract is obtained by collecting and freeze-drying mesenchymal stem cells obtained from bovine bone marrow tissue through serum-free culture.

2. The method for preparing the oil-control liquid composition having the function of tightening pores according to claim 1, characterized in that: The following steps are involved: A1: The preparation of mesenchymal stem cell extract includes three steps: obtaining mesenchymal stem cells, culturing mesenchymal stem cells, and collecting and freeze-drying them; A2: Add tea tree essential oil, honey mud and salicylic acid into pure water and place in a magnetic stirrer, heat in a water bath, set the temperature to 65°C, set the speed to 1200 rpm, and stir for 30 minutes to obtain material A; A3: Mix and dissolve the purslane extract, rosemary extract and MSC extract in purified water, add sodium hyaluronate, place the mixture on a magnetic stirrer, heat in a water bath, set the temperature to 40°C, set the speed to 600 rpm, and stir for 30 minutes to obtain material B; A4: Cool material A and material B to room temperature, and after cooling to room temperature, mix material A and material B and place them in a magnetic therapy stirrer, set the speed to 600 rpm and stir for 2 hours to obtain an oil control liquid composition.

3. The method for preparing the oil-control liquid composition having the function of tightening pores according to claim 2, characterized in that: The obtaining of mesenchymal stem cells comprises the following steps: B1: Mix ampicillin, kanamycin and streptomycin at a concentration of 1 / 1000 to prepare a triple antibody; soak healthy bovine bone marrow tissue in 75% disinfectant alcohol, wash for 15 minutes, then take out the bovine bone marrow tissue and transfer it to the triple antibody and soak for 30 minutes; B2: After soaking, transfer the bovine bone marrow tissue to sterile water and soak and wash for 15 minutes; B3: Place the processed bovine bone marrow tissue into 10% bovine serum DMEM culture medium, use a sterile glass slide to press the bovine bone marrow tissue to the bottom of the culture dish for normal culture, observe once a day, change the culture medium every two days, and wait for the MSC cells to crawl out from the bottom of the culture dish.

4. The method for preparing the oil-control liquid composition having the function of tightening pores according to claim 2, characterized in that: The mesenchymal stem cell culture comprises the following steps: C1: Wait for a large number of MSC cells to form colonies at the bottom of the culture dish before removing the bovine bone marrow tissue; C2: Use 9% trypsin to digest and passage MSC cells. When the MSC cells have completed passage and have grown all over the bottom of the culture dish, replace the DMEM medium with serum-free medium and culture for 24 hours.

5. The method for preparing the oil-control liquid composition having the function of tightening pores according to claim 2, characterized in that: The collection and lyophilization comprises the following steps: D1: MSC cells were digested normally and frozen in a liquid nitrogen tank; D2: Collect serum-free medium after culture completion; D3: Add 5% mannitol to the culture medium, the ratio of serum-free culture medium to 5% mannitol is 20:1, mix well and place in a freeze dryer for 48 hours, and collect the MSC extract freeze-dried powder.

6. The oil-control liquid composition with pore-tightening function according to claim 1, characterized in that: The molecular weight of the sodium hyaluronate is 6000-8000 Daltons.

7. The oil-control liquid composition with pore-tightening function according to claim 1, characterized in that: The purslane extract is water-soluble purslane extract powder with a specification of 15:

1.

8. The oil-control liquid composition with pore-tightening function according to claim 1, characterized in that: The rosemary extract is water-soluble rosemary extract powder with a specification of 15:1.

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