Biological selenium conversion method crop nutrient solution and preparation method of organic selenium biological extracting solution of component of biological selenium conversion method crop nutrient solution
The bioconversion of inorganic selenium into organic form using edible fungi enhances crop selenium absorption and utilization, improving crop yield and selenium content, with residual nutrients acting as a fertilizer.
Patent Information
- Application Number
- CN202411460045.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-18
- Publication Date
- 2024-12-27
AI Technical Summary
Existing inorganic and organic selenium fertilizers face challenges in achieving full absorption by crops due to various factors, leading to suboptimal selenium utilization in agricultural production.
A bioconversion process using edible fungi to convert inorganic selenium sources into organic selenium, combined with a nutrient solution base, enhancing crop absorption through a bio-selenium conversion crop nutrient solution.
The method improves selenium absorption and utilization in crops by converting inorganic selenium to organic form, increasing crop yield and selenium content, with residual culture medium serving as a root fertilizer for further benefits.
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Figure CN119191888A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of agriculture, and in particular to a method for preparing a bio-selenium-converted crop nutrient solution and an organic selenium biological extract solution as a component thereof. Background Art
[0002] Selenium is one of the essential trace elements in human life activities. It cannot be synthesized in the human body. The total selenium content of the human body is 6-20 mg. The highest concentration is in the kidney. It is an antioxidant in the human body and is very important for improving human immunity and preventing cancer. At the same time, selenium also has multiple biological functions. Urinary selenium is a good indicator for judging the surplus and deficiency of selenium in the human body. Since there is no organ in the human body that can store selenium for a long time, the selenium required by the body should be continuously obtained from the diet. The balance of selenium concentration has an important protective and promoting effect on the physiological functions of many organs and tissues.
[0003] The selenium content in most soils in my country is relatively low. In the agricultural production process, inorganic selenium fertilizers and organic selenium nutrient solutions are usually used to increase the selenium content of crops. However, the absorption of selenium in inorganic selenium fertilizers and organic selenium nutrient solutions by crops is affected by many factors. In most cases, the applied selenium cannot be fully absorbed. Therefore, it is urgent to develop a selenium nutrient solution containing inorganic selenium and organic selenium with better crop absorption effect to improve the absorption and utilization rate of selenium by crops. Summary of the invention
[0004] Based on the above technical background, the present invention provides a method for preparing a bio-selenium crop nutrient solution and its component organic selenium biological extract solution to solve the above problems.
[0005] The present invention is achieved through the following technical solutions: A method for preparing an organic selenium biological extract, the steps of preparing the organic selenium biological extract and a nutrient solution base solution by a bioconversion process include: A. Select sodium selenate, sodium selenite or a mixture thereof as an inorganic selenium source; B. Mix 70% of grains, 20% of pine needle powder, 6% of corn flour, 1% of superphosphate and 1% of sugar, add water and cook until done. After cooling, mix the inorganic selenium source with the mixture at a ratio of 5‰-8‰ to prepare a culture medium. C. Mixing the edible fungus strains with the culture medium to make fungus sticks, placing them in a fungus room, and cultivating them for 1-3 months until they grow into edible fungi with high selenium content and then collecting them; D. After the mature edible fungi are collected and cleaned, they are added into a crusher with a same weight of water and stirred thoroughly to completely break the cell walls into a paste, and the liquid in the paste of edible fungi is squeezed out by squeezing, and the squeezed liquid is filtered to obtain the organic selenium biological extract described in the present invention.
[0006] E. Mix the used mushroom stick culture medium and the squeezed edible mushroom residue, add 3-5 times of clean water, stir well for 1-2 hours in a constant temperature reactor at 60-70° C., and then filter to obtain the nutrient solution base liquid of the present invention.
[0007] A bio-selenium conversion crop nutrient solution, the components of which are measured by weight as follows: Organic selenium biological extract 100-150g, nutrient solution base 800-900g, hydrated citric acid 1-2g, sodium selenite 1-2g, organosilicon 1-2g, potassium chloride 2-5g, amino acid raw powder 2-5g, potassium dihydrogen phosphate 1-2g; During the preparation, the nutrient solution base liquid is first added into a stirring barrel, and then the organic selenium biological extract, hydrated citric acid, sodium selenite, organosilicon, potassium chloride and potassium dihydrogen phosphate are sequentially added into the stirring barrel and stirred evenly to obtain the bio-selenium conversion method crop nutrient solution described in the present invention.
[0008] Preferably, the edible fungi are white button mushrooms and straw mushrooms which have good selenium absorption and fast growth rate.
[0009] Preferably, the residue after preparing the nutrient solution base liquid can be used as root fertilizer for plants. Beneficial Effects
[0010] Through the bioconversion process, edible fungi are used to convert inorganic selenium sources into organic selenium that is more easily absorbed by crops, and the cell walls of edible fungi are broken to extract high-concentration organic selenium biological extracts; The used culture medium and the squeezed edible fungus residue are added with water for a second time, stirred and filtered to obtain a solution as a nutrient solution base liquid, and the inorganic selenium and organic selenium remaining in the culture medium and the squeezed edible fungus residue are fully beneficial, so that the prepared crop nutrient solution contains both inorganic selenium and organic selenium, which is beneficial to the absorption of selenium by crops, and a small amount of sodium selenite is added during the preparation to increase the content of inorganic selenium in the nutrient solution; The residue after preparing the nutrient solution base liquid can also be used as root fertilizer for plants. The residual selenium and organic matter are beneficial to the growth of crops and further improve the utilization rate of selenium. BRIEF DESCRIPTION OF THE DRAWINGS
[0011] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings: Figure 1 It is a comparison chart of various indicators of Example 1, Example 2, Example 3 and the control example of the present application. DETAILED DESCRIPTION
[0012] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with embodiments. The illustrative embodiments of the present invention and their description are only used to explain the present invention and are not intended to limit the present invention.
[0013] A wheat field was selected as the object of the embodiment for a control experiment. Four ridges in the same wheat field were selected as the test site. The same wheat seeds and fertilizers were used. The only difference between the corresponding embodiments 1, 2, 3 and the control example was the use of the bio-selenium crop nutrient solution. Example 1
[0014] A method for preparing an organic selenium biological extract, the steps of preparing the organic selenium biological extract and a nutrient solution base solution by a bioconversion process include: A. Selecting a mixture of sodium selenate and sodium selenite as an inorganic selenium source; B. Mix 70% of grains, 20% of pine needle powder, 6% of corn flour, 1% of superphosphate and 1% of sugar, add water and cook until done. After cooling, mix the inorganic selenium source with the mixture at a ratio of 8‰ to prepare a culture medium. C. Mixing the mushroom spawn with the culture medium to make mushroom sticks, placing them in a mushroom room, and culturing them for one month until they grow into edible mushrooms with high selenium content and then collecting them; D. After collecting and cleaning the mature small white mushrooms, adding the mixture with the same weight of water into a crusher and fully stirring to completely break the cell walls into a paste, squeezing out the liquid in the paste-like edible fungi, and filtering the squeezed liquid to obtain the organic selenium biological extract described in the present invention.
[0015] E. Mix the used mushroom culture medium and the squeezed white mushroom residue, add 3 times of clean water, stir well for 1-2 hours in a constant temperature reactor at 60-70° C., and filter to obtain the nutrient solution base liquid of the present invention.
[0016] A bio-selenium conversion crop nutrient solution, the components of which are measured by weight as follows: 150g organic selenium biological extract, 800g nutrient solution base, 2g hydrated citric acid, 2g sodium selenite, 2g organosilicon, 5g potassium chloride, 2g potassium dihydrogen phosphate; During the preparation, the nutrient solution base liquid is first added into a stirring barrel, and then the organic selenium biological extract, hydrated citric acid, sodium selenite, organosilicon, potassium chloride and potassium dihydrogen phosphate are sequentially added into the stirring barrel and stirred evenly to obtain the bio-selenium conversion method crop nutrient solution described in the present invention.
[0017] When in use, during the wheat sowing period, the residue after preparing the nutrient solution base liquid is used as wheat root fertilizer, and the bio-selenium crop nutrient solution is sprayed on the experimental wheat ridges three times during the wheat heading stage and grain filling stage, with a dosage of 250g / mu. Example 2
[0018] A method for preparing an organic selenium biological extract, the steps of preparing the organic selenium biological extract and a nutrient solution base solution by a bioconversion process include: A. Selecting a mixture of sodium selenate and sodium selenite as an inorganic selenium source; B. Mix 70% of grains, 20% of pine needle powder, 6% of corn flour, 1% of superphosphate and 1% of sugar, add water and cook until done. After cooling, mix the inorganic selenium source with the mixture at a ratio of 8‰ to prepare a culture medium. C. Mixing the mushroom spawn with the culture medium to make mushroom sticks, placing them in a mushroom room, and culturing them for one month until they grow into edible mushrooms with high selenium content and then collecting them; D. After collecting and cleaning the mature small white mushrooms, adding the mixture with the same weight of water into a crusher and fully stirring to completely break the cell walls into a paste, squeezing out the liquid in the paste-like edible fungi, and filtering the squeezed liquid to obtain the organic selenium biological extract described in the present invention.
[0019] E. Mix the used mushroom culture medium and the squeezed white mushroom residue, add 3 times of clean water, stir well for 1-2 hours in a constant temperature reactor at 60-70° C., and filter to obtain the nutrient solution base liquid of the present invention.
[0020] A bio-selenium conversion crop nutrient solution, the components of which are measured by weight as follows: 150g organic selenium biological extract, 800g nutrient solution base, 2g hydrated citric acid, 2g sodium selenite, 2g organosilicon, 5g potassium chloride, 2g potassium dihydrogen phosphate; During the preparation, the nutrient solution base liquid is first added into a stirring barrel, and then the organic selenium biological extract, hydrated citric acid, sodium selenite, organosilicon, potassium chloride and potassium dihydrogen phosphate are sequentially added into the stirring barrel and stirred evenly to obtain the bio-selenium conversion method crop nutrient solution described in the present invention.
[0021] When in use, the bio-selenium crop nutrient solution was sprayed on the experimental wheat ridges three times during the wheat heading stage, grain filling stage and 10 days after the grain filling stage, with the dosage being 250g / mu. Example 3
[0022] A method for preparing an organic selenium biological extract, the steps of preparing the organic selenium biological extract and a nutrient solution base solution by a bioconversion process include: A. Selecting a mixture of sodium selenate and sodium selenite as an inorganic selenium source; B. Mix 70% of grains, 20% of pine needle powder, 6% of corn flour, 1% of superphosphate and 1% of sugar, add water and cook until done. After cooling, mix the inorganic selenium source with the mixture at a ratio of 5‰ to prepare a culture medium. C. Mixing the mushroom spawn with the culture medium to make mushroom sticks, placing them in a mushroom room, and culturing them for one month until they grow into edible mushrooms with high selenium content and then collecting them; D. After collecting and cleaning the mature small white mushrooms, adding the mixture with the same weight of water into a crusher and fully stirring to completely break the cell walls into a paste, squeezing out the liquid in the paste-like edible fungi, and filtering the squeezed liquid to obtain the organic selenium biological extract described in the present invention.
[0023] E. After mixing the used mushroom stick culture medium and the squeezed white mushroom residue, add 5 times of clean water, stir well for 1-2 hours in a constant temperature reactor at 60-70° C., and filter to obtain the nutrient solution base liquid of the present invention.
[0024] A bio-selenium conversion crop nutrient solution, the components of which are measured by weight as follows: 150g organic selenium biological extract, 800g nutrient solution base, 2g hydrated citric acid, 2g sodium selenite, 2g organosilicon, 5g potassium chloride, 2g potassium dihydrogen phosphate; During preparation, first add the nutrient solution base liquid into a stirring barrel, and then sequentially add the organic selenium biological extract, hydrated citric acid, sodium selenite, silicone, potassium chloride, and potassium dihydrogen phosphate into the stirring barrel and stir evenly to obtain the bio-selenium crop nutrient solution described in the present invention. The nutrient solution prepared in this embodiment has a lower concentration than that in Embodiment 1-2.
[0025] When in use, the bio-selenium crop nutrient solution was sprayed on the experimental wheat ridges three times during the wheat heading stage, grain filling stage and 10 days after the grain filling stage, with the dosage being 250g / mu.
[0026] According to comparative experiments, it was found that the concentration and dosage of the bio-selenium-converting crop nutrient solution in the present invention have a significant effect on improving the selenium content and yield of wheat; the use of the base liquid residue as a fertilizer also significantly improves the selenium content and yield of wheat.
[0027] The specific implementation methods described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific implementation method of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A method for preparing an organic selenium biological extract, characterized in that: The steps of preparing organic selenium biological extract and nutrient solution base solution by bioconversion process include: A. Select sodium selenate, sodium selenite or a mixture thereof as an inorganic selenium source; B. Mix 70% of grains, 20% of pine needle powder, 6% of corn flour, 1% of superphosphate and 1% of sugar, add water, cook and cool, and fully mix the inorganic selenium source with the mixture at a ratio of 5‰-8‰ to prepare a culture medium; C. Mixing the edible fungus strains with the culture medium to form fungus sticks, placing them in a fungus room, and cultivating them for 1-3 months until they grow into edible fungi with high selenium content and then collecting them; D. After collecting and cleaning the mature edible fungi, adding the mixture with water of the same weight into a crusher and stirring thoroughly to completely break the cell walls into a paste, squeezing out the liquid in the paste-like edible fungi, and filtering the squeezed liquid to obtain the organic selenium biological extract of the present invention; E. Mix the used mushroom stick culture medium and the squeezed edible mushroom residue, add 3-5 times of clean water, stir well for 1-2 hours in a constant temperature reactor at 60-70° C., and then filter to obtain the nutrient solution base liquid of the present invention.
2. A bio-selenium-converting crop nutrient solution, characterized in that: The components by weight are as follows: 100-150 g of the organic selenium biological extract prepared in claim 1, 800-900 g of the nutrient solution base prepared in claim 1, 1-2 g of hydrated citric acid, 1-2 g of sodium selenite, 1-2 g of organosilicon, 2-5 g of potassium chloride, 2-5 g of amino acid raw powder, and 1-2 g of potassium dihydrogen phosphate; During preparation, first add the nutrient solution base liquid into a mixing barrel, and then sequentially add the organic selenium biological extract, hydrated citric acid, sodium selenite, organosilicon, potassium chloride and potassium dihydrogen phosphate into the mixing barrel and stir evenly.
3. The method for preparing an organic selenium biological extract according to claim 1, characterized in that: The edible fungi used are white mushrooms and straw mushrooms which have good selenium absorption and fast growth rate.
4. The method for preparing an organic selenium biological extract according to claim 1, characterized in that: The residue after preparing the nutrient solution base liquid is used as root fertilizer for plants.
