Preparation and use method of antibacterial foam-stabilizing difunctional modified biomass preparation for radioactive contamination decontamination agent

Abstract

The invention discloses a preparation and use method of an antibacterial and foam-stabilizing dual-function modified biomass preparation for a radioactive contamination decontamination agent, and the preparation method comprises the following steps: adding a biomass material and a modifier into a solvent, and stirring for reaction to obtain a biomass modified product; transferring the biomass modified product into a dialysis bag for dialysis, collecting the product, and freeze-drying to obtain the antibacterial foam-stabilizing dual-function modified biomass preparation. The use method comprises the following steps: uniformly mixing and stirring the antibacterial and foam-stabilizing dual-function modified biomass preparation, the surfactant and the deionized water to obtain the modified biomass-based radioactive contamination decontaminating agent. According to the modified biomass-based radioactive contamination decontamination agent and the preparation method thereof, a biomass material is modified, so that the modified biomass material has double functions of inhibiting bacteria and stabilizing foam, the modified biomass preparation is added into the decontamination agent, and the prepared modified biomass-based radioactive contamination decontamination agent has good antibacterial activity, foam stability and radioactive contamination decontamination performance; and the hydrogel has the advantages of no skin irritation, good biocompatibility, less secondary pollution, environmental friendliness and the like.

Description

Technical Field

[0001] The present invention belongs to the field of biomass material utilization, and more specifically, relates to a preparation and use method of a modified biomass preparation with dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant. Background Art

[0002] In the practical application of cleaning and decontamination technology, the storage stability and foam stability of the cleaning agent are crucial. The stability of the foam not only directly affects the effect of cleaning and decontamination, but is also closely related to the secondary pollution problem that may be caused by the foam bursting. The storage stability of the cleaning agent affects the storage time and service life of the cleaning agent. Therefore, in order to effectively increase the storage stability and foam stability of the cleaning agent, improve the cleaning and decontamination efficiency, and reduce the risk of secondary pollution to the environment after the foam bursts, researchers usually add foam stabilizers and antibacterial agents to the cleaning agent.

[0003] Among these foam stabilizers, natural polymer foam stabilizers have attracted widespread attention due to their unique environmental friendliness. This foam stabilizer is not only derived from nature and is renewable, but also contains some active functional groups in its molecular structure that can adsorb and enrich radioactive nuclides. This makes natural polymer foam stabilizers show significant advantages in removing radioactive contamination. However, the antibacterial ability of existing natural polymer foam stabilizers is relatively weak. Without the addition of antibacterial agents, the disinfectant is prone to breed bacteria during long-term storage or use, resulting in performance degradation and even deterioration. Due to the existence of these limiting factors, the scope of natural polymer foam stabilizers in practical applications is greatly limited. Therefore, it is of great significance to study a biomass preparation with dual functions of antibacterial and foam stabilization for the application of disinfectants. Summary of the invention

[0004] An object of the present invention is to solve at least the above problems and / or disadvantages and to provide at least the advantages which will be described hereinafter.

[0005] In order to achieve these purposes and other advantages of the present invention, a method for preparing a modified biomass preparation having dual functions of antibacterial and foam-stabilizing for use in a radioactive contamination disinfectant is provided, comprising the following steps:

[0006] Step 1: mixing the biomass material and the solvent, stirring and dissolving to obtain a biomass solution, adding the modifier to the biomass solution, stirring and reacting to obtain a biomass modified product;

[0007] Step 2: Transfer the biomass modified product to a dialysis bag, place the dialysis bag in deionized water, dialyze, and then collect the product in the dialysis bag, freeze-dry, and obtain a modified biomass preparation with dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant.

[0008] Preferably, in step 1, the biomass material is one or more of konjac glucomannan, gelatin, chitosan, xanthan gum, hyaluronic acid, bletilla striata polysaccharide, collagen, carrageenan, and cellulose; and the solvent is deionized water.

[0009] Preferably, in step 1, the modifier is one or more of 2,3-epoxypropyltrimethylammonium chloride, acryloyloxyethyltrimethylammonium chloride, glycidyltrimethylammonium chloride, L-arginine, N-hydroxysuccinimide, 1-ethyl-(3-dimethylaminopropyl)carbodiimide and p-biguanidinobenzoyl chloride.

[0010] Preferably, in step 1, the specific conditions for stirring and dissolving are: temperature 20-80° C., using hydrochloric acid aqueous solution or sodium hydroxide aqueous solution to adjust pH=2-7, stirring speed 300-800 r / min, time 0.5-1 h.

[0011] Preferably, in step 1, the specific conditions for the stirring reaction are: temperature 20-110° C., using hydrochloric acid aqueous solution or sodium hydroxide aqueous solution to adjust pH=2-8.5, stirring speed 600-1500 r / min, time 5-48 h.

[0012] Preferably, in step 1, the concentration of the biomass solution is 0.5-3wt%; and the mass ratio of the biomass material to the modifier is 1:1-7.

[0013] Preferably, in step 2, the dialysis time is 72 h to 96 h; and the freeze-drying time is 24 h to 48 h.

[0014] A method for using the antibacterial and foam-stabilizing dual-functional modified biomass preparation for radioactive contamination disinfectant prepared by the preparation method as described above comprises: using the antibacterial and foam-stabilizing dual-functional modified biomass preparation to prepare a modified biomass-based radioactive contamination disinfectant.

[0015] Preferably, the method for preparing the modified biomass-based radioactive contamination cleaning agent is specifically as follows: mixing the antibacterial and foam-stabilizing dual-functional modified biomass preparation, a surfactant and deionized water, and stirring them evenly to obtain the modified biomass-based radioactive contamination cleaning agent.

[0016] Preferably, the surfactant is one or more of sodium N-lauroyl sarcosinate, cocamidopropyl betaine, tea saponin, alkyl glucoside, sodium α-olefin sulfonate, sodium dodecylbenzene sulfonate, hexadecyltrimethylammonium bromide, and tetradecyl sulfopropyl betaine.

[0017] Preferably, the mass ratio of the antibacterial and foam-stabilizing dual-function modified biomass preparation to the surfactant is 1 to 20:1; and in the modified biomass-based radioactive contamination disinfectant, the concentration of the surfactant is 0.005 to 2 wt%.

[0018] Preferably, before using the antibacterial and foam-stabilizing dual-functional modified biomass preparation, the antibacterial and foam-stabilizing dual-functional modified biomass preparation is pretreated, and the specific method of the pretreatment is: adding the antibacterial and foam-stabilizing dual-functional modified biomass preparation and bayberry tannin into deionized water, ultrasonically dispersing for 40 to 80 minutes at 40 to 60kHz and 300 to 500W, then adding an ethanol solution of dodecanol, stirring for 1 to 3 hours, adding the obtained mixed solution into a polyamide-polypropylene composite bag for vacuum packaging, controlling the vacuum degree to 0.1MPa, placing it in a high static pressure equipment, performing a cyclic high static pressure treatment, and then freeze-drying for 36 to 48 hours to obtain the pretreated antibacterial and foam-stabilizing dual-functional modified biomass preparation.

[0019] Preferably, the mass volume ratio of the antibacterial and foam-stabilizing dual-functional modified biomass preparation, bayberry tannin and deionized water is 5-15g:0.5-3g:100-300mL; the concentration of the ethanol solution of dodecanol is 0.05-0.2g / mL; the mass ratio of the antibacterial and foam-stabilizing dual-functional modified biomass preparation and dodecanol is 5-15:1-3.

[0020] Preferably, the specific conditions of the cyclic high static pressure treatment are: the treatment temperature is 20-30°C, the pressure is first increased to 400-600MPa, and the pressure is maintained for 4-6min, then the pressure is reduced to 100-200MPa, and the pressure is maintained for 4-6min, and finally the pressure is increased to 400-600MPa, and the pressure is maintained for 4-6min, and the pressure increase or reduction rate is 5-10MPa / min.

[0021] The present invention includes at least the following beneficial effects: the present invention uses resource-rich, renewable, degradable, and environmentally friendly biomass materials as raw materials, modifies them so that they have dual functions of antibacterial and foam stabilization, thereby solving the problem that the existing natural polymer foam stabilizers have relatively weak antibacterial ability. The modified biomass preparation is added to the disinfectant, and the prepared modified biomass-based radioactive contamination disinfectant has good antibacterial properties, foam stability and radioactive contamination disinfection performance, and also has the advantages of no skin irritation, good biocompatibility, less secondary pollution, and environmental friendliness; in addition, the preparation method of the present invention is simple to operate, environmentally friendly, and has good application prospects.

[0022] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The infrared spectrum of the quaternized konjac glucomannan prepared in Example 1 of the present invention;

[0024] Figure 2 The SEM electron micrographs of Escherichia coli and Staphylococcus aureus before and after treatment with the quaternary ammonium konjac glucomannan prepared in Example 1 of the present invention;

[0025] Figure 3 This is a comparison chart of the antibacterial activity of quaternized konjac glucomannan (N-KGM) and konjac glucomannan (KGM) prepared in Example 1 of the present invention against Escherichia coli and Staphylococcus aureus;

[0026] Figure 4 A comparison chart of the drainage half-life of the modified biomass-based radioactive contamination decontamination agents prepared in Examples 1 to 3 of the present invention and the decontamination agent prepared in Comparative Example 1;

[0027] Figure 5 This is a comparison chart of the decontamination performance of the modified biomass-based radioactive contamination decontamination agent prepared in Example 1 of the present invention and the decontamination agent prepared in Comparative Example 1;

[0028] Figure 6 The infrared spectrum of the quaternized gelatin prepared in Example 4 of the present invention;

[0029] Figure 7 The infrared spectrum of the quaternized chitosan prepared in Example 5 of the present invention;

[0030] Figure 8 This is a comparison chart of the decontamination performance of the modified biomass-based radioactive contamination decontamination agents prepared in Examples 1, 9 to 11 of the present invention. DETAILED DESCRIPTION

[0031] The present invention is further described in detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0032] It should be understood that the terms such as “having”, “including” and “comprising” used herein do not exclude the existence or addition of one or more other elements or combinations thereof.

[0033] Example 1

[0034] A method for preparing a modified biomass preparation having dual functions of antibacterial and foam-stabilizing for use in a radioactive contamination cleaning agent comprises the following steps:

[0035] Step 1, dissolving 1g of konjac glucomannan in deionized water at a pH of 7, a temperature of 25°C, and a stirring speed of 600r / min to obtain a konjac glucomannan solution with a concentration of 1wt%, adding 3mL of an aqueous solution of 0.5g / mL acryloyloxyethyltrimethylammonium chloride to the konjac glucomannan solution, reacting for 15h at a temperature of 70°C, a pH of 7, and a stirring speed of 800r / min to obtain a modified konjac glucomannan product; wherein, a hydrochloric acid aqueous solution or a sodium hydroxide aqueous solution is used to adjust the pH;

[0036] Step 2, transferring the modified konjac glucomannan product to a dialysis bag, placing the dialysis bag in deionized water, and dialyzing for 72 hours. After the dialysis is completed, the product in the dialysis bag is collected and freeze-dried for 48 hours to obtain a bacteriostatic and foam-stabilizing dual-function modified biomass preparation for radioactive contamination disinfectant, i.e., quaternized konjac glucomannan;

[0037] Its usage includes:

[0038] The quaternary ammonium konjac glucomannan, alkyl glycoside and deionized water are uniformly mixed to obtain a modified biomass-based radioactive contamination cleaning agent, wherein the concentration of the alkyl glycoside is 1 wt % and the concentration of the quaternary ammonium konjac glucomannan is 1 wt %.

[0039] Example 2

[0040] In this embodiment, the concentration of quaternized konjac glucomannan in the modified biomass-based radioactive contamination disinfectant is 2 wt %, and the remaining steps are the same as those in Embodiment 1.

[0041] Example 3

[0042] In this embodiment, the concentration of quaternized konjac glucomannan in the modified biomass-based radioactive contamination disinfectant is 3 wt %, and the remaining steps are the same as those in Embodiment 1.

[0043] Comparative Example 1

[0044] The alkyl polyglycoside and deionized water are uniformly mixed to obtain a cleaning agent, wherein the concentration of the alkyl polyglycoside is 1 wt %, and no modified biomass preparation, namely quaternized konjac glucomannan, is added.

[0045] Figure 1 The infrared spectra of quaternized konjac glucomannan (N-KGM) and konjac glucomannan (KGM) prepared in Example 1 are shown in Figure 3410 cm -1 , 2936cm -1 , 1643cm -1 The vibration peaks at the bottom are the -OH stretching vibration peak of konjac glucomannan, -CH 2The stretching vibration peak of - and the bending vibration absorption peak of hydroxyl group. Comparing with the infrared spectrum of quaternized konjac glucomannan (N-KGM), a new absorption peak appears at 1479 cm -1 . This is the C-N absorption peak on the quaternary ammonium salt, indicating that there are quaternary ammonium salt groups on the molecular chain of konjac glucomannan.

[0046] Using the quaternized konjac glucomannan prepared in Example 1 to treat Escherichia coli and Staphylococcus aureus: Inoculate the bacterial strains into NB medium and culture at 37 °C for 12 h. Adjust the concentration to 1×10 7 cfu / mL. Take 10 mL of the bacterial suspension and add quaternized konjac glucomannan at 1 MIC concentration. All samples are placed at 37 °C for 3 h. Take out the bacterial liquid and rinse it twice with phosphate buffer (PBS, pH 7.4). Add glutaraldehyde solution and fix at 4 °C for 4 h. Dehydrate with alcohol solutions of gradient concentrations (30%, 50%, 70%, 80%, 90%, 100%), replace alcohol with tert-butanol, coat the film and observe under SEM. The results are as Figure 2 shown. Figure 2 (a, b) are untreated Escherichia coli and Staphylococcus aureus bacterial cells. The surfaces of the untreated bacterial cells are smooth and complete, without obvious scratches and cracks; Figure 2 (c, d) are Escherichia coli and Staphylococcus aureus bacterial cells treated with quaternized konjac glucomannan at 1 MIC concentration. The surfaces of the bacterial cells show wrinkles, depressions, and even cell lysis. The antibacterial mechanism of quaternized konjac glucomannan is to damage the cell membrane, resulting in the leakage of intracellular substances, affecting the growth of bacteria, and thus achieving the antibacterial purpose.

[0047] Determination of the antibacterial zone by the disk diffusion method: Weigh quaternized konjac glucomannan and prepare a sample solution with a concentration of 2.00 g / mL with distilled water; Make round filter paper with a diameter of 6 mm, sterilize, dry, and reserve; Prepare a sterile LB medium plate with a diameter of 90 mm and reserve; Take 100 μL of the bacterial suspension with a concentration of 3×10 5 CFU / mL and apply it evenly on the LB medium plate with a spreading rod to make a bacteria-containing plate; Stick the sterilized round filter paper in a "product" shape on the bacteria-containing plate. Sterile distilled water is used as the blank control, konjac glucomannan is used as the negative control, and quaternized konjac glucomannan is used as the sample. Add 20 μL of the solution to each round filter paper. After treatment, place each plate in a constant temperature incubator and culture at 37 °C for 18 h, then observe whether there is an antibacterial zone, and measure the diameter size (mm) with a ruler. If there is no bacterial growth at the edge of the filter paper and a transparent antibacterial zone is formed, it is judged to have antibacterial activity. Repeat the parallel experiment three times. The results are as Figure 3As shown. It can be seen that on the Escherichia coli and Staphylococcus aureus plates, an antibacterial zone appeared around the filter paper with quaternary ammonium konjac glucomannan (N-KGM) added, indicating that quaternary ammonium konjac glucomannan has antibacterial activity against both Escherichia coli and Staphylococcus aureus.

[0048] The foam volume of the disinfectant was tested by the Waring Blender method: 50 mL of disinfectant was taken and foamed at 3000 r / min for 2 min, the foam was placed in a graduated cylinder, and the volume was read to evaluate the foaming ability. The time when the foam drainage volume in the graduated cylinder reached 25 mL was the drainage half-life. Comparison of drainage half-lives of the modified biomass-based radioactive contamination disinfectants prepared in Examples 1 to 3 and the disinfectant prepared in Comparative Example 1 Figure 4 Shown, it can be seen that the drainage half-life of Comparative Example 1 (not adding quaternized konjac glucomannan) is 300s, the drainage half-life of Example 1 (containing 1wt% quaternized konjac glucomannan) is 1910s, the drainage half-life of Example 2 (containing 2wt% quaternized konjac glucomannan) is 3450s, and the drainage half-life of Example 3 (containing 3wt% quaternized konjac glucomannan) is 4500s. After adding quaternized konjac glucomannan, the foam drainage half-life of the cleaning agent all significantly increases, indicating that this material has good foam stabilizing performance.

[0049] Decontamination experiment: Take 1 mL of 10 g / L uranyl nitrate solution and evenly apply it on a 10 cm*10 cm plate (glass plate, stainless steel plate, tile), then age it for 14 days to obtain a contaminated sample. Use a surface radioactive contamination meter (FJ-2207) to measure the radioactive counting rate at a constant distance of 0.5 cm. The radioactive counting rate before contamination is A 0 The radioactivity count rate after contamination is A 1 ; Foam the disinfectant at 3000r / min for 2min, spray it on the contaminated sample surface, let it stand for 45min, recover the foam with a foam recovery device, heat the decontaminated surface and dry it at 50℃, then cool it to room temperature to test the radioactivity count rate, which is recorded as A 2 The decontamination factor DE of uranyl ions is calculated according to the following formula:

[0050]

[0051] Figure 5The comparison chart of the decontamination factors of the modified biomass-based radioactive contamination decontamination agent prepared in Example 1 and the decontamination agent prepared in Comparative Example 1 shows that, compared with Comparative Example 1, the decontamination factors of Example 1 are respectively increased by 3.13, 1.86, and 1.95 times. This is because the quaternized konjac glucomannan increases the foam stability of the decontamination agent and increases the action time of the decontamination agent with pollutants, so that the decontamination agent can better wet and dissolve pollutants on the solid surface, and carry the pollutants through the foam to remove them from the surface.

[0052] Example 4

[0053] A method for preparing a modified biomass preparation having dual functions of antibacterial and foam-stabilizing for use in a radioactive contamination cleaning agent comprises the following steps:

[0054] Step 1: Dissolve 1 g of gelatin in deionized water at a pH of 7, a temperature of 50° C., and a stirring speed of 600 r / min to obtain a gelatin solution with a concentration of 1 wt%. Add 3 mL of a 0.5 g / mL aqueous solution of trimethylammonium glycidyl chloride to the gelatin solution, and react for 5 hours at a temperature of 60° C., a pH of 8.5, and a stirring speed of 800 r / min to obtain a modified gelatin product.

[0055] Step 2: Transfer the modified gelatin product to a dialysis bag, place the dialysis bag in deionized water, and dialyze for 72 hours. After the dialysis is completed, collect the product in the dialysis bag, freeze-dry for 36 hours, and obtain a modified biomass preparation with antibacterial and foam-stabilizing functions for radioactive contamination disinfectant, namely, quaternized gelatin;

[0056] Its usage includes:

[0057] The quaternary ammonium gelatin, alkyl glycoside and deionized water are uniformly mixed to obtain a modified biomass-based radioactive contamination cleaning agent, wherein the concentration of the alkyl glycoside is 1wt% and the concentration of the quaternary ammonium gelatin is 2wt%.

[0058] Figure 6 The infrared spectra of the quaternized gelatin and gelatin prepared in Example 4, c is gelatin, d is quaternized gelatin. -1 The absorption band corresponding to the deformation of the primary amine NH group on the gelatin chain almost disappeared, while the absorption band at 1478 cm -1 The absorption band of CH functional group of quaternary ammonium methyl substituent appeared at 1097cm -1 and 1053cm -1 The absorption band intensity at 240 nm increases, and these absorption bonds are attributed to the symmetric stretching of COC and the skeleton vibration of CO stretching, respectively.

[0059] Example 5

[0060] A method for preparing a modified biomass preparation having dual functions of antibacterial and foam-stabilizing for use in a radioactive contamination cleaning agent comprises the following steps:

[0061] Step 1: dissolving 1 g of chitosan in deionized water at a pH of 2, a temperature of 80° C., and a stirring speed of 800 r / min to obtain a chitosan solution having a concentration of 1 wt%, adding 3 mL of an aqueous solution of 0.5 g / mL trimethylammonium chloride glycidyl chloride to the chitosan solution, and reacting for 12 h at a temperature of 110° C., a pH of 2, and a stirring speed of 800 r / min to obtain a modified chitosan product;

[0062] Step 2: Transfer the modified chitosan product to a dialysis bag, place the dialysis bag in deionized water, and dialyze for 72 hours. After the dialysis is completed, collect the product in the dialysis bag, freeze-dry for 24 hours, and obtain a modified biomass preparation with antibacterial and foam-stabilizing functions for radioactive contamination disinfectant, namely, quaternized chitosan;

[0063] Its usage includes:

[0064] The quaternary ammonium chitosan, alkyl glycoside and deionized water are uniformly mixed to obtain a modified biomass-based radioactive contamination cleaning agent, wherein the concentration of the alkyl glycoside is 0.5wt% and the concentration of the quaternary ammonium chitosan is 1wt%.

[0065] Figure 7 The infrared spectra of the quaternized chitosan and chitosan prepared in Example 5, a is chitosan, b is quaternized chitosan. -1 The absorption band corresponding to the deformation of the primary NH group on the chitosan chain almost disappeared, while the absorption band at 1479 cm -1 The absorption band of CH functional group of quaternary ammonium methyl substituent appeared at the position of the chitosan nanostructured carbonyl. The results showed that quaternary ammonium substituent was successfully introduced into chitosan.

[0066] Example 6

[0067] A method for preparing a modified biomass preparation having dual functions of antibacterial and foam-stabilizing for use in a radioactive contamination cleaning agent comprises the following steps:

[0068] Step 1, dissolving 1g of xanthan gum in deionized water at a pH of 7, a temperature of 25°C, and a stirring speed of 600r / min to obtain a xanthan gum solution with a concentration of 1wt%, adding 3mL of an aqueous solution of 0.5g / mL acryloyloxyethyltrimethylammonium chloride to the xanthan gum solution, reacting for 5h at a temperature of 70°C, a pH of 8.5, and a stirring speed of 800r / min to obtain a modified xanthan gum product;

[0069] Step 2: Transfer the modified xanthan gum product to a dialysis bag, place the dialysis bag in deionized water, and dialyze for 72 hours. After the dialysis is completed, collect the product in the dialysis bag, freeze-dry for 48 hours, and obtain a modified biomass preparation with antibacterial and foam-stabilizing functions for radioactive contamination disinfectant, namely, quaternized xanthan gum;

[0070] Its usage includes:

[0071] The quaternary ammonium xanthan gum, hexadecyltrimethylammonium bromide and deionized water are uniformly mixed to obtain a modified biomass-based radioactive contamination cleaning agent, wherein the concentration of the alkyl glycoside is 0.05wt%, and the concentration of the quaternary ammonium xanthan gum is 0.25wt%.

[0072] Example 7

[0073] A method for preparing a modified biomass preparation having dual functions of antibacterial and foam-stabilizing for use in a radioactive contamination cleaning agent comprises the following steps:

[0074] Step 1, dissolving 1g of collagen in deionized water at a pH of 7, a temperature of 20°C, and a stirring speed of 800r / min to obtain a collagen solution with a concentration of 1wt%, adding 2g of L-arginine, 2g of N-hydroxysuccinimide and 3g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide to the collagen solution, reacting for 5h at a temperature of 20°C, a pH of 6.5, and a stirring speed of 800r / min to obtain a modified collagen product;

[0075] Step 2: Transfer the modified collagen product to a dialysis bag, place the dialysis bag in deionized water, and dialyze for 72 hours. After the dialysis is completed, collect the product in the dialysis bag, freeze-dry for 48 hours, and obtain a bacteriostatic and foam-stabilizing dual-functional modified biomass preparation for radioactive contamination disinfectant, namely, amidated collagen;

[0076] Its usage includes:

[0077] The amidated collagen, hexadecyltrimethylammonium bromide and deionized water are uniformly mixed to obtain a modified biomass-based radioactive contamination cleaning agent, wherein the concentration of the alkyl glycoside is 0.05wt%, and the concentration of the amidated collagen is 0.15wt%.

[0078] Example 8

[0079] A method for preparing a modified biomass preparation having dual functions of antibacterial and foam-stabilizing for use in a radioactive contamination cleaning agent comprises the following steps:

[0080] Step 1, dissolving 1g of Bletilla striata polysaccharide in deionized water at a pH of 7, a temperature of 25°C, and a stirring speed of 600r / min to obtain a Bletilla striata polysaccharide solution with a concentration of 1wt%, adding 2g of L-arginine, 2g of N-hydroxysuccinimide and 3g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide to the Bletilla striata polysaccharide solution, reacting for 5h at a temperature of 20°C, a pH of 6.5, and a stirring speed of 800r / min to obtain a modified Bletilla striata polysaccharide product;

[0081] Step 2: Transfer the modified Bletilla striata polysaccharide product to a dialysis bag, place the dialysis bag in deionized water, and dialyze for 72 hours. After the dialysis is completed, collect the product in the dialysis bag, freeze-dry for 48 hours, and obtain an antibacterial and foam-stabilizing dual-functional modified biomass preparation for radioactive contamination disinfectant, namely, amidated Bletilla striata polysaccharide;

[0082] Its usage includes:

[0083] The amidated Bletilla striata polysaccharide, alkyl glycoside and deionized water are uniformly mixed to obtain a modified biomass-based radioactive contamination cleaning agent, wherein the concentration of the alkyl glycoside is 0.5wt%, and the concentration of the amidated Bletilla striata polysaccharide is 1wt%.

[0084] Example 9

[0085] In the present embodiment, before using quaternized konjac glucomannan, it is pretreated, and the remaining steps are the same as in Example 1; the specific method of the pretreatment of the quaternized konjac glucomannan is: 10g quaternized konjac glucomannan and 1g bayberry tannin are added to 200mL deionized water, ultrasonically dispersed at 55kHz, 400W for 60min, then 25mL 0.1g / mL ethanol solution of dodecanol is added, stirred for 2h, the mixed solution obtained is added to a polyamide-polypropylene composite bag and vacuum packaged, the vacuum degree is controlled to be 0.1MPa, placed in a high static pressure device, circulated high static pressure treatment, then freeze-dried for 48h, to obtain pretreated quaternized konjac glucomannan; the specific conditions of the cyclic high static pressure treatment are: the treatment temperature is 25°C, first boosted to 500MPa, pressure-maintained for 5min, then reduced to 100MPa, pressure-maintained for 5min, finally boosted to 500MPa, pressure-maintained for 5min, and the boost or depressurization speed is 8MP a / min.

[0086] In this embodiment, dodecanol and bayberry tannin are used to pretreat quaternary ammonium konjac glucomannan, the active sites are increased, the adsorption and binding capacity with heavy metals is enhanced, and the modified biomass-based radioactive contamination disinfectant obtained has better stability. The mixed liquid is subjected to cyclic high static pressure treatment using high static pressure equipment, and a large number of active groups are generated under the action of high-low-high pressure, which effectively improves the reaction activity, thereby further improving the disinfection and decontamination performance of the disinfectant.

[0087] Example 10

[0088] In the present embodiment, before using the quaternized konjac glucomannan, it is pretreated, and the remaining steps are the same as in Example 1; the specific method of the quaternized konjac glucomannan pretreatment is: 10g of quaternized konjac glucomannan and 1g of bayberry tannin are added to 200mL of deionized water, ultrasonically dispersed at 55kHz, 400W for 60min, then 25mL of 0.1g / mL ethanol solution of dodecanol is added, stirred for 2h, the obtained mixed solution is added to a polyamide-polypropylene composite bag for vacuum packaging, the vacuum degree is controlled to 0.1MPa, placed in a high static pressure device, high static pressure treatment is carried out, and then freeze-dried for 48h to obtain the pretreated quaternized konjac glucomannan; the specific conditions of the high static pressure treatment are: the treatment temperature is 25°C, the pressure is increased to 500MPa, the pressure is maintained for 15min, and the pressure increase rate is 8MPa / min.

[0089] Embodiment 11

[0090] In this embodiment, the quaternized konjac glucomannan is pretreated before use, and the remaining steps are the same as those in Example 1; the specific method for the pretreatment of the quaternized konjac glucomannan is as follows: 10 g of quaternized konjac glucomannan and 1 g of bayberry tannin are added to 200 mL of deionized water, ultrasonically dispersed at 55 kHz and 400 W for 60 min, then 25 mL of 0.1 g / mL dodecanol ethanol solution is added, stirred for 2 h, and freeze-dried for 48 h to obtain the pretreated quaternized konjac glucomannan.

[0091] Figure 8 The comparison chart of the decontamination factors of the modified biomass-based radioactive contamination decontamination agents prepared in Examples 1 and 9 to 11 shows that, compared with Example 1, the decontamination performance of the modified biomass-based radioactive contamination decontamination agents of Examples 9 to 11 are all improved, among which Example 9 has the highest performance.

[0092] Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and the implementation modes, and they can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the present invention is not limited to the specific details and the illustrations shown and described herein.

Claims

1. A method for preparing a modified biomass preparation having dual functions of antibacterial and foam-stabilizing for use as a radioactive contamination disinfectant, characterized in that: The following steps are involved: Step 1: mixing the biomass material and the solvent, stirring and dissolving to obtain a biomass solution, adding the modifier to the biomass solution, stirring and reacting to obtain a biomass modified product; Step 2: Transfer the biomass modified product to a dialysis bag, place the dialysis bag in deionized water, dialyze, and then collect the product in the dialysis bag, freeze-dry, and obtain a modified biomass preparation with dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant.

2. The method for preparing a modified biomass preparation with dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant according to claim 1, characterized in that: In the step 1, the biomass material is one or more of konjac glucomannan, gelatin, chitosan, xanthan gum, hyaluronic acid, bletilla striata polysaccharide, collagen, carrageenan, and cellulose; and the solvent is deionized water.

3. The method for preparing a modified biomass preparation with dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant according to claim 1, characterized in that: In the step 1, the modifier is one or more of 2,3-epoxypropyltrimethylammonium chloride, acryloyloxyethyltrimethylammonium chloride, glycidyltrimethylammonium chloride, L-arginine, N-hydroxysuccinimide, 1-ethyl-(3-dimethylaminopropyl)carbodiimide and p-biguanidinobenzoyl chloride.

4. The method for preparing a modified biomass preparation having dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant according to claim 1, characterized in that: In the step 1, the specific conditions for stirring and dissolving are: temperature 20-80°C, pH=2-7, stirring speed 300-800r / min, and time 0.5-1h; the specific conditions for stirring reaction are: temperature 20-110°C, pH=2-8.5, stirring speed 600-1500r / min, and time 5-48h.

5. The method for preparing a modified biomass preparation with dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant according to claim 1, characterized in that: In the step 1, the concentration of the biomass solution is 0.5-3wt%; the mass ratio of the biomass material to the modifier is 1:1-7.

6. The method for preparing a modified biomass preparation with dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant according to claim 1, characterized in that: In the step 2, the dialysis time is 72h to 96h; and the freeze-drying time is 24h to 48h.

7. A method for using the antibacterial and foam-stabilizing dual-function modified biomass preparation for radioactive contamination disinfectant prepared by the preparation method according to any one of claims 1 to 6, characterized in that: include: The modified biomass-based radioactive contamination disinfectant is prepared by using the antibacterial and foam-stabilizing dual-functional modified biomass preparation.

8. The method for using the modified biomass preparation with dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant according to claim 7, characterized in that: The preparation method of the modified biomass-based radioactive contamination cleaning agent specifically comprises: mixing the antibacterial and foam-stabilizing dual-functional modified biomass preparation, a surfactant and deionized water, and stirring them evenly to obtain the modified biomass-based radioactive contamination cleaning agent.

9. The method for using the modified biomass preparation with dual functions of antibacterial and foam-stabilizing for radioactive contamination disinfectant according to claim 8, characterized in that: The surfactant is one or more of sodium N-lauroyl sarcosinate, cocamidopropyl betaine, tea saponin, alkyl glucoside, sodium α-olefin sulfonate, sodium dodecylbenzene sulfonate, hexadecyltrimethylammonium bromide, and tetradecyl sulfopropyl betaine.

10. The method for using the modified biomass preparation with dual functions of antibacterial and foam stabilizing for radioactive contamination disinfectant according to claim 8, characterized in that: The mass ratio of the modified biomass preparation with dual functions of antibacterial and foam-stabilizing to the surfactant is 1 to 20:1; in the modified biomass-based radioactive contamination disinfectant, the concentration of the surfactant is 0.005 to 2 wt%.

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