A method for debonding fertilized eggs of marine fish

Abstract

The present invention provides a method for debonding fertilized eggs of marine fish, comprising the following steps: S1. immersion and disinfection of fertilized eggs; S2. preparation of debonding liquid; S3. debonding of fertilized eggs; S4. separation and cleaning of fertilized eggs. Before debonding fertilized eggs of marine fish, the present invention first uses a composite Chinese herbal medicine preparation to soak the fertilized eggs of marine fish to kill bacteria and fungi in the fertilized eggs of marine fish. Avoid infection of fertilized eggs of marine fish during the debonding process, thereby improving the subsequent hatching rate of fertilized eggs of marine fish. The present invention uses a debonding agent composed of a specific proportion of disodium hydrogen phosphate, water, a composite enzyme solution, and cystine to debond fertilized eggs of marine fish. The debonding agent of the present invention is mild and non-irritating, and can effectively avoid damage to fertilized eggs of marine fish caused by mechanical vibration and friction in traditional debonding methods.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a method for debonding fertilized eggs of marine fish. Background Art

[0002] When the fertilized eggs of marine fish are just laid, their surface is usually covered with a layer of mucus. The presence of mucus will cause the fertilized eggs to stick together, resulting in the obstruction of oxygen and nutrient exchange, which in turn affects the normal development of the embryo. Secondly, during the incubation process, if the fertilized eggs are severely adhered, some fertilized eggs will die due to lack of sufficient oxygen, reducing the hatching rate. Therefore, when artificially incubating fertilized eggs of marine fish, debonding treatment is required. Debonding treatment can effectively remove the mucus on the surface of the fertilized eggs, keep the fertilized eggs in a dispersed state, and ensure that each fertilized egg can get sufficient oxygen and nutrient supply, which can significantly improve the hatching rate of the fertilized eggs. However, the traditional debonding method mainly uses mechanical vibration and friction to remove the sticky substances on the surface of the fertilized eggs, which can easily damage the fertilized eggs. After the fertilized eggs are damaged, they are prone to bacterial and fungal infections, resulting in a decrease in the hatching rate in the later stage. Summary of the invention

[0003] In view of this, the present invention proposes a method for debonding fertilized eggs of marine fish to solve the above-mentioned problem.

[0004] The technical solution of the present invention is achieved as follows: a method for debonding fertilized eggs of marine fish, comprising the following steps:

[0005] S1. Soaking and disinfecting fertilized eggs: Soaking the collected fertilized eggs of marine fish in a composite Chinese herbal medicine preparation, wherein the composite Chinese herbal medicine preparation includes the following ingredients: salvia miltiorrhiza, astragalus, honeysuckle, angelica, atractylodes, and forsythia;

[0006] S2. Preparation of demucin solution: disodium hydrogen phosphate, water, complex enzyme solution, and cystine are weighed in proportion, disodium hydrogen phosphate is added to water and stirred until dissolved, and then the complex enzyme solution and cystine are added and stirred evenly, and insoluble matter is filtered out with a 80-100 mesh sieve to obtain a demucin solution; wherein the complex enzyme solution is composed of a trypsin solution, a cellulase solution, and a pectinase solution;

[0007] S3. Fertilized egg debonding: Add the sterilized marine fish fertilized eggs into the debonding solution and stir gently with a feather until the marine fish fertilized eggs become shiny and transparent;

[0008] S4. Separation and cleaning of fertilized eggs: After debonding, the fertilized eggs of marine fish are transferred to a separation container, into which sodium chloride solution is added and allowed to stand; high-quality fertilized eggs float on the water surface, while low-quality fertilized eggs sink to the bottom of the separation container; the fertilized eggs of marine fish on the water surface are collected with a sieve, and cleaned with clean seawater to remove the sodium chloride solution on the surface of the fertilized eggs. The cleaned fertilized eggs of marine fish can be hatched.

[0009] Furthermore, the mass volume ratio of marine fish fertilized eggs to the composite Chinese herbal medicine preparation in S1 is 1.0:(1.5-2.0) in g / mL, and the soaking time is 20-40 min.

[0010] Furthermore, the weight ratio of Astragalus, Salvia miltiorrhiza, Honeysuckle, Angelica, Forsythia and Atractylodes in the composite Chinese herbal preparation in S1 is (4-5):(3-4):(2-3):(2-3):(2-3):(1-2).

[0011] Furthermore, the composite Chinese herbal preparation in S1 is prepared by the following method: Astragalus, Salvia miltiorrhiza, Honeysuckle, Angelica sinensis, Forsythia suspensa, and Atractylodes macrocephala are mixed in a weight ratio, and purified water with a mass volume ratio of g / mL (1.0-1.5): (3.0-5.0) is added for decoction, and after boiling, the mixture is kept in a slightly boiling state for 25-35 minutes, and the residue is filtered out with an 80-100 mesh sieve to obtain a composite Chinese herbal extract, and purified water 6-8 times its volume is added to the composite Chinese herbal extract, and the mixture is stirred evenly to obtain a composite Chinese herbal preparation.

[0012] Furthermore, in S2, the stirring speed is 200-300 r / min and the stirring time is 3-5 min.

[0013] Furthermore, the mass volume ratio of disodium hydrogen phosphate, water, complex enzyme solution and cysteine ​​in S2 is (3-5):(8-10):(2-3):(1-2), wherein the volume ratio of trypsin solution, cellulase solution and pectinase solution in the complex enzyme solution is (1-3):(1-3):(1-2).

[0014] Furthermore, the trypsin activity in the trypsin solution is 100-150 U / mL, the cellulase activity in the cellulase solution is 50-100 U / mL, and the pectinase activity in the pectinase solution is 30-80 U / mL.

[0015] Furthermore, in S3, the mass volume ratio of marine fish fertilized eggs to demucin g / mL is 1.0:(1.3-1.7), and the demucin treatment is performed for 20-40s.

[0016] Furthermore, the mass volume ratio of marine fish fertilized eggs to sodium chloride solution in S4 is 1:(4-6) g / mL, wherein the concentration of the sodium chloride solution is 3.0-3.6%wt, and the standing time is 10-20min.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: before debonding the fertilized eggs of marine fish, the present invention first uses a composite Chinese herbal preparation to soak the fertilized eggs of marine fish to kill bacteria and fungi in the fertilized eggs of marine fish. Avoid infection of the fertilized eggs of marine fish during the debonding process, thereby improving the subsequent hatching rate of the fertilized eggs of marine fish. The Chinese herbal preparation of the present invention can not only effectively resist bacteria and inflammation, but also promote the flow of body fluids in the cells of fertilized eggs of marine fish, thereby promoting the development of fertilized eggs of marine fish. At the same time, the Chinese herbal preparation contains rich antioxidants, which can resist the damage of oxidative stress to fertilized eggs. And the Chinese herbal preparation can also enhance the immunity of fertilized eggs of marine fish, which helps the fertilized eggs of marine fish resist the invasion of external pathogens.

[0018] The present invention uses a debonding agent composed of a specific proportion of disodium hydrogen phosphate, water, a composite enzyme solution, and cystine to debond marine fish fertilized eggs. The debonding agent of the present invention is mild and non-irritating, and can effectively avoid the damage to marine fish fertilized eggs caused by mechanical vibration and friction in traditional debonding methods. The composite enzyme solution in the debonding agent of the present invention can effectively decompose mucin and mucopolysaccharide in the mucus of marine fish fertilized eggs, and the disodium hydrogen phosphate therein provides a suitable environment for trypsin, cellulase, and pectinase in the composite enzyme solution, thereby improving the activity of the enzyme, thereby promoting the composite enzyme solution to decompose the mucus of marine fish fertilized eggs. The cystine in the debonding agent helps to reduce the surface tension of the solution, making it easier for the debonding agent to penetrate into the tiny gaps on the surface of marine fish fertilized eggs. In addition, the amino and carboxyl functional groups in cystine can form hydrogen bonds with the hydroxyl and carboxyl functional groups in the mucopolysaccharide molecules. These hydrogen bonds can weaken the interaction between mucopolysaccharide molecules, thereby destroying their viscous structure. Electrostatic interaction also occurs between cystine and mucopolysaccharide. Since mucopolysaccharides usually have a negative charge, and the amino group in cystine has a positive charge, an electrostatic attraction will be generated between them. This electrostatic attraction will interfere with the normal interaction between mucopolysaccharide molecules and further destroy their viscous structure. Therefore, the present invention combines disodium hydrogen phosphate, water, complex enzyme solution, and cystine in a specific ratio, and each component acts synergistically through different mechanisms to gently and efficiently debond fertilized marine fish eggs. DETAILED DESCRIPTION

[0019] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0020] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0021] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources. Example 1

[0022] A method for debonding fertilized eggs of marine fish comprises the following steps:

[0023] S1. Soaking and disinfection of fertilized eggs: The collected fertilized eggs of marine fish are placed in a composite Chinese herbal preparation for soaking, wherein the mass-to-volume ratio of the fertilized eggs of marine fish to the composite Chinese herbal preparation (g / mL) is 1.0:1.5, the soaking time is 20 min, and the composite Chinese herbal preparation is composed of astragalus, salvia miltiorrhiza, honeysuckle, angelica, forsythia and atractylodes in a weight ratio of 4:3:2:2:2:1; the composite Chinese herbal preparation is prepared by the following method: Astragalus, salvia miltiorrhiza, honeysuckle, angelica, forsythia and atractylodes are mixed according to a weight ratio, and pure water with a mass-to-volume ratio of g / mL1.0:3.0 is added for decoction, and after boiling, the mixture is kept in a slightly boiling state for 25 min, and the residue is filtered out with an 80-mesh sieve to obtain a composite Chinese herbal extract, and pure water 6 times its volume is added to the composite Chinese herbal extract, and the mixture is stirred evenly to obtain the composite Chinese herbal preparation.

[0024] S2. Preparation of demucining liquid: weigh disodium hydrogen phosphate, water, complex enzyme solution and cystine in proportion, wherein the mass volume ratio of disodium hydrogen phosphate, water, complex enzyme solution and cystine is 3:8:2:1 g / mL; add disodium hydrogen phosphate to water and stir until dissolved; then add complex enzyme solution and cystine and stir at 200 r / min for 5 min; filter out insoluble matter with an 80-mesh sieve to obtain demucining liquid; wherein the complex enzyme solution is composed of trypsin solution, cellulase solution and pectinase solution in a volume ratio of 1:1:1; and the trypsin activity in the trypsin solution is 100 U / mL, the cellulase activity in the cellulase solution is 50 U / mL, and the pectinase activity in the pectinase solution is 30 U / mL.

[0025] S3. Fertilized egg debonding: Add the disinfected marine fish fertilized eggs into the debonding solution, with the mass volume ratio of marine fish fertilized eggs to debonding solution being 1.0:1.3 g / mL, and stir gently with a feather. Debond for 40 seconds until the marine fish fertilized eggs become bright and transparent;

[0026] S4. Separation and cleaning of fertilized eggs: After debonding, the fertilized eggs of marine fish are transferred to a separation container, and a sodium chloride solution with a concentration of 3.0%wt is added to the separation container. The mass volume ratio of the fertilized eggs of marine fish to the sodium chloride solution (g / mL) is 1:4. The mixture is allowed to stand for 20 minutes. High-quality fertilized eggs float on the water surface, while low-quality fertilized eggs sink to the bottom of the separation container. The fertilized eggs of marine fish on the water surface are collected with a sieve and cleaned with clean seawater to remove the sodium chloride solution on the surface of the fertilized eggs. The cleaned fertilized eggs of marine fish can be hatched. Example 2

[0027] A method for debonding fertilized eggs of marine fish comprises the following steps:

[0028] S1. Soaking and disinfection of fertilized eggs: The collected fertilized eggs of marine fish are placed in a composite Chinese herbal preparation for soaking, wherein the mass volume ratio of the fertilized eggs of marine fish to the composite Chinese herbal preparation is 1.0:1.8 g / mL, and the soaking time is 30 min. The composite Chinese herbal preparation is composed of astragalus, salvia miltiorrhiza, honeysuckle, angelica, forsythia and atractylodes in a weight ratio of 4.5:3.5:2.5:2.5:2.5:1.5; the composite Chinese herbal preparation is prepared by the following method: Astragalus, salvia miltiorrhiza, honeysuckle, angelica, forsythia and atractylodes are mixed according to a weight ratio, and purified water with a mass volume ratio of 1.2:3.5 is added for decoction, and after boiling, the mixture is kept in a slightly boiling state for 30 min, and the residue is filtered out with a 90-mesh sieve to obtain a composite Chinese herbal extract, and purified water 7 times its volume is added to the composite Chinese herbal extract, and the mixture is stirred evenly to obtain the composite Chinese herbal preparation.

[0029] S2. Preparation of demucining liquid: weigh disodium hydrogen phosphate, water, complex enzyme solution and cystine in proportion, wherein the mass volume ratio of disodium hydrogen phosphate, water, complex enzyme solution and cystine is 4.0:9.0:2.5:1.5 g / mL; add disodium hydrogen phosphate to water and stir until dissolved; then add complex enzyme solution and cystine and stir at 250 r / min for 4 min; filter out insoluble matter with a 90-mesh sieve to obtain demucining liquid; wherein the complex enzyme solution is composed of trypsin solution, cellulase solution and pectinase solution in a volume ratio of 2:2:1.5; and the trypsin activity in the trypsin solution is 125 U / mL, the cellulase activity in the cellulase solution is 75 U / mL, and the pectinase activity in the pectinase solution is 55 U / mL.

[0030] S3. Fertilized egg debonding: Add the disinfected marine fish fertilized eggs into the debonding solution, with the mass volume ratio of marine fish fertilized eggs to debonding solution being 1.0:1.5 g / mL, stir gently with a feather, and debond for 30 seconds until the marine fish fertilized eggs become bright and transparent;

[0031] S4. Separation and cleaning of fertilized eggs: The debonded fertilized eggs of marine fish are transferred to a separation container, and a sodium chloride solution with a concentration of 3.3%wt is added to the separation container. The mass volume ratio of the fertilized eggs of marine fish to the sodium chloride solution (g / mL) is 1:5. The mixture is allowed to stand for 15 minutes. High-quality fertilized eggs float on the water surface, while low-quality fertilized eggs sink to the bottom of the separation container. The fertilized eggs of marine fish on the water surface are collected with a sieve and cleaned with clean seawater to remove the sodium chloride solution on the surface of the fertilized eggs. The cleaned fertilized eggs of marine fish can be hatched. Example 3

[0032] A method for debonding fertilized eggs of marine fish comprises the following steps:

[0033] S1. Soaking and disinfection of fertilized eggs: The collected fertilized eggs of marine fish are placed in a composite Chinese herbal preparation for soaking, wherein the mass-to-volume ratio of the fertilized eggs of marine fish to the composite Chinese herbal preparation g / mL is 1.0:2.0, the soaking time is 40 min, and the composite Chinese herbal preparation is composed of astragalus, salvia miltiorrhiza, honeysuckle, angelica, forsythia and atractylodes in a weight ratio of 5:4:3:3:3:2; the composite Chinese herbal preparation is prepared by the following method: astragalus, salvia miltiorrhiza, honeysuckle, angelica, forsythia and atractylodes are mixed according to a weight ratio, pure water with a mass-to-volume ratio of g / mL1.5:4.0 is added and boiled, and after boiling, the mixture is kept in a slightly boiling state and boiled for 35 min, and the residue is filtered out with a 100-mesh sieve to obtain a composite Chinese herbal extract, and pure water 8 times its volume is added to the composite Chinese herbal extract, and the mixture is stirred evenly to obtain the composite Chinese herbal preparation.

[0034] S2. Preparation of demucin solution: weigh disodium hydrogen phosphate, water, complex enzyme solution and cystine in proportion, wherein the mass volume ratio of disodium hydrogen phosphate, water, complex enzyme solution and cystine is 5:10:3:2 g / mL; add disodium hydrogen phosphate to water and stir until dissolved; then add complex enzyme solution and cystine and stir at 300 r / min for 3 min; filter out insoluble matter with a 100-mesh sieve to obtain a demucin solution; wherein the complex enzyme solution is composed of trypsin solution, cellulase solution and pectinase solution in a volume ratio of 3:3:2; and the trypsin activity in the trypsin solution is 150 U / mL, the cellulase activity in the cellulase solution is 100 U / mL, and the pectinase activity in the pectinase solution is 80 U / mL.

[0035] S3. Fertilized egg debonding: Add the disinfected marine fish fertilized eggs into the debonding solution, with the mass volume ratio of marine fish fertilized eggs to debonding solution being 1.0:1.7 g / mL, and stir gently with a feather. Debond for 20 seconds until the marine fish fertilized eggs become bright and transparent;

[0036] S4. Separation and cleaning of fertilized eggs: The debonded fertilized eggs of marine fish are transferred to a separation container, and a sodium chloride solution with a concentration of 3.6%wt is added to the separation container. The mass volume ratio of the fertilized eggs of marine fish to the sodium chloride solution (g / mL) is 1:6. The mixture is allowed to stand for 10 minutes. High-quality fertilized eggs float on the water surface, while low-quality fertilized eggs sink to the bottom of the separation container. The fertilized eggs of marine fish on the water surface are collected with a sieve and cleaned with clean seawater to remove the sodium chloride solution on the surface of the fertilized eggs. The cleaned fertilized eggs of marine fish can be hatched.

[0037] Comparative Example 1

[0038] The difference between this comparative example and Example 2 is that the detackifying agent is composed of water and complex enzyme solution in a volume ratio of 9.0:2.5.

[0039] Comparative Example 2

[0040] The difference between this comparative example and Example 2 is that the mass volume ratio of disodium hydrogen phosphate, water, complex enzyme solution and cystine in the detackifying agent is 6:12:1:0.5 g / mL.

[0041] Comparative Example 3

[0042] The difference between this comparative example and Example 2 is that a single trypsin solution is used instead of an equal volume of a composite protease solution.

[0043] Comparative Example 4

[0044] The difference between this comparative example and Example 2 is that no Chinese herbal medicine preparation is used for soaking before debonding.

[0045] Comparative Example 5

[0046] Compared with Example 2, the difference between this comparative example and Example 2 is that the Chinese herbal medicine preparation consists of Astragalus, Honeysuckle and Forsythia in a weight ratio of 5:3:3.

[0047] In order to verify the effect of the debonding method of the fertilized eggs of marine fish of the present invention, the following experiment is conducted: the fertilized eggs of marine fish that have been debonded and cleaned according to the debonding methods of Examples 1-3 and Comparative Examples 1-5 are laid flat in the hatching frames, and 1000 fertilized eggs of marine fish are laid in each hatching frame. Three hatching frames are set in each embodiment and each comparative example. The hatching frame is made of a PVC pipe with a diameter of 25 mm and a 40-mesh screen silk net. The hatching frame floats in the hatching pool. The hatching pool adopts microporous aeration and the dissolved oxygen is 6.5 mg / L. The hatching pool is equipped with heating facilities to keep the hatching water temperature at about 26°C. After 24 hours, the necrotic eggs are identified by the whitish and opaque characteristics. The necrotic eggs are removed in time and the good egg rate is counted. After 65 hours, the number of fry in each group is counted and the hatching rate is counted. The good egg rate and hatching rate are taken as the average value of the three frames, and the statistical results are shown in Table 1. The calculation formula of the good egg rate and hatching rate is shown in the following formula:

[0048]

[0049]

[0050] Table 1

[0051] Good egg rate% Hatching rate% Example 1 93.7 87.6 Example 2 95.3 90.1 Example 3 93.2 88.5 Comparative Example 1 66.4 63.7 Comparative Example 2 63.9 61.5 Comparative Example 3 70.3 68.9 Comparative Example 4 68.3 65.4 Comparative Example 5 73.0 70.8

[0052] It can be seen from Table 1 that the debonding method of the present invention can safely and efficiently debond fertilized marine fish eggs during the debonding process, and through the disinfection of fertilized eggs and the promotion of fertilized eggs development, the good egg rate and hatching rate obtained by the debonding method are higher, especially Example 2 has a better effect.

[0053] Compared with Comparative Example 1, the disodium hydrogen phosphate in the debonding agent of Example 2 can adjust the pH of the solution. During the debonding process, the appropriate acid-base environment helps the complex enzyme solution to exert optimal activity, thereby improving the debonding efficiency. In addition, disodium hydrogen phosphate also has a buffering effect, which can stabilize the pH value of the solution and avoid adverse effects on the debonding process due to pH fluctuations. The cystine has a sulfhydryl group that reacts with the mucin in the mucus to reduce the viscosity of the mucus, thereby helping to debond; in addition, the amino and carboxyl functional groups in cystine can form hydrogen bonds with the hydroxyl and carboxyl functional groups in the mucopolysaccharide molecules. These hydrogen bonds can weaken the interaction between mucopolysaccharide molecules, thereby destroying their viscous structure. Electrostatic interactions also occur between cystine and mucopolysaccharides. Since mucopolysaccharides are usually negatively charged, and the amino groups in cystine are positively charged, electrostatic attraction will be generated between them. This electrostatic attraction will interfere with the normal interaction between mucopolysaccharide molecules and further destroy their viscous structure. Under the suitable acid-base environment provided by disodium hydrogen phosphate and the synergistic effect of cystine, the activity of the complex enzyme solution is fully exerted, and a synergistic effect is formed between various enzymes, which greatly improves the debonding effect and further improves the good egg rate and hatching rate.

[0054] Compared with Comparative Example 2, in order to achieve a better debonding effect, the ratio of disodium hydrogen phosphate, water, complex enzyme solution and cystine needs to be strictly controlled within a certain range. Beyond this ratio range, the balance between the various components will be broken, resulting in a significant reduction in the debonding effect.

[0055] Compared with Example 3, Example 2 shows that a single trypsin solution can only decompose mucin in the mucus, while the composite enzyme solution composed of a trypsin solution with specific activity, a cellulase solution, and a pectinase solution can not only effectively decompose mucin, but also effectively decompose the mucopolysaccharides in the mucus, and the decomposition of the mucus is more thorough, and the debonding effect is better. Therefore, the good egg rate and hatching rate are also higher.

[0056] Compared with Comparative Example 4, the bioactive ingredients such as chlorogenic acid, luteolin, forsythiaside, forsythiaphenol contained in the Chinese herbal preparation have a broad-spectrum antibacterial effect and have an inhibitory effect on a variety of bacteria and viruses. Soaking in a Chinese herbal preparation before debonding can effectively disinfect the surface of fertilized eggs of marine fish, reduce or kill the existing pathogenic microorganisms, thereby reducing the risk of embryonic death due to bacterial infection during subsequent hatching. The astragalus polysaccharide in the Chinese herbal preparation has good moisturizing properties, can effectively lock in moisture, and provide a moist microenvironment for the fertilized eggs. Astragaloside, volatile oil, organic acid and ferulic acid in the Chinese herbal preparation help regulate and improve microcirculation, provide a better nutrient supply environment for fertilized eggs, and its antioxidant effect also helps protect fertilized eggs from damage, all of which help to improve the survival rate and hatching rate of fertilized eggs of marine fish. The proteins, amino acids, vitamins astragaline, forsythine and other ingredients contained in Chinese herbal preparations are absorbed through the surface of fertilized eggs of marine fish, which have a positive effect on embryonic development, stimulate embryonic development, increase embryonic vitality, and thus improve the hatching rate.

[0057] Compared with Comparative Example 5, the combination of Astragalus and Angelica in the Chinese herbal medicine preparation of Example 2 can replenish qi and blood, and improve the overall condition of fertilized eggs of marine fish; the combination of Salvia miltiorrhiza and Honeysuckle can promote blood circulation and remove blood stasis, clear away heat and detoxify, and create a better internal environment for the embryonic development of fertilized eggs of marine fish. When these Chinese herbal medicines are used in combination, they can produce a synergistic effect and enhance each other's efficacy. Therefore, the Chinese herbal medicine preparation composed of Astragalus, Salvia miltiorrhiza, Honeysuckle, Angelica, Forsythia, and Atractylodes can improve the good egg rate and hatching rate through more comprehensive pharmacodynamic effects and synergistic effects of pharmacodynamics. The comprehensive conditioning effect of this preparation is more significant than that of the preparation composed only of Astragalus, Honeysuckle, and Forsythia.

[0058] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for debonding fertilized eggs of marine fish, characterized in that: The following steps are involved: S1. Soaking and disinfecting fertilized eggs: Soak the collected marine fish fertilized eggs in a composite Chinese herbal medicine preparation, wherein the mass volume ratio of the marine fish fertilized eggs to the composite Chinese herbal medicine preparation is 1.0:(1.5-2.0) g / mL, and the soaking time is 20-40 min. The composite Chinese herbal medicine preparation comprises the following ingredients: salvia miltiorrhiza, astragalus, honeysuckle, angelica, atractylodes, and forsythia, and the weight ratio of astragalus, salvia miltiorrhiza, honeysuckle, angelica, forsythia, and atractylodes is (4-5):(3-4):(2-3):(2-3):(2-3):(1-2); S2. Preparation of demucin solution: Weigh disodium hydrogen phosphate, water, complex enzyme solution, and cystine in proportion, wherein the mass volume ratio of disodium hydrogen phosphate, water, complex enzyme solution, and cystine is (3-5):(8-10):(2-3):(1-2) g / mL, add disodium hydrogen phosphate to water and stir until dissolved, then add complex enzyme solution and cystine and stir evenly, filter out insoluble matter with a 80-100 mesh sieve to obtain demucin solution; The composite enzyme solution is composed of a trypsin solution, a cellulase solution, and a pectinase solution, and the volume ratio of the trypsin solution, the cellulase solution, and the pectinase solution is (1-3):(1-3):(1-2), the trypsin activity in the trypsin solution is 100-150 U / mL, the cellulase activity in the cellulase solution is 50-100 U / mL, and the pectinase activity in the pectinase solution is 30-80 U / mL; S3. Debonding of fertilized eggs: Add the disinfected marine fish fertilized eggs to the debonding solution and stir gently with a feather until the marine fish fertilized eggs become bright and transparent. The debonding is stopped when the mass volume ratio of the marine fish fertilized eggs to the debonding solution is 1.0:(1.3-1.7) g / mL. The debonding treatment is performed for 20-40s. S4. Separation and cleaning of fertilized eggs: After debonding, the fertilized eggs of marine fish are transferred to a separation container, into which sodium chloride solution is added and allowed to stand; high-quality fertilized eggs float on the water surface, while low-quality fertilized eggs sink to the bottom of the separation container; the fertilized eggs of marine fish on the water surface are collected with a sieve, and cleaned with clean seawater to remove the sodium chloride solution on the surface of the fertilized eggs. The cleaned fertilized eggs of marine fish can be hatched.

2. A method for debonding fertilized marine fish eggs as claimed in claim 1, characterized in that: The composite Chinese herbal preparation in S1 is prepared by the following method: Astragalus, Salvia miltiorrhiza, Honeysuckle, Angelica sinensis, Forsythia suspensa, and Atractylodes macrocephala are mixed in a weight ratio, and purified water with a mass-to-volume ratio of g / mL (1.0-1.5): (3.0-5.0) is added for decoction, and after boiling, the mixture is kept in a slightly boiling state for 25-35 minutes, and the residue is filtered out with an 80-100 mesh sieve to obtain a composite Chinese herbal extract, and purified water 6-8 times the volume of the composite Chinese herbal extract is added to the composite Chinese herbal extract, and the mixture is stirred evenly to obtain the composite Chinese herbal preparation.

3. A method for debonding fertilized marine fish eggs as claimed in claim 1, characterized in that: In the S2, the stirring speed is 200-300 r / min and the stirring time is 3-5 min.

4. A method for debonding fertilized marine fish eggs as claimed in claim 1, characterized in that: The mass volume ratio of the fertilized marine fish eggs to the sodium chloride solution in S4 is 1:(4-6) g / mL, the concentration of the sodium chloride solution is 3.0-3.6%wt, and the standing time is 10-20min.