Red blood cell simulant as well as preparation method and application thereof

By pretreatment, homogenization, repair and fixation of red blood cells in urine, red blood cell mimetics with good uniformity and stability were prepared, which solved the problems of uneven morphology and poor stability of existing quality control substances, and improved the Accuracy and reliability of red blood cell detection in urine.

CN119193481APending Publication Date: 2024-12-27SICHUAN MACCURA BIOTECH CO LTD
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Patent Information

Application Number
CN202411143524.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-08-20
Publication Date
2024-12-27

AI Technical Summary

Technical Problem

The existing quality control substances used for erythrocyte analysis in urine have problems such as uneven morphology, poor stability, large batch differences, and high production costs, which limit the accuracy and reliability of erythrocyte detection in urine.

Method used

By pretreatment, homogenization, cell repair and fixation of mammalian whole blood, red blood cell mimics were prepared, and high osmotic reagents containing surfactant and specific components of cell repair solution and fixative are improved. Uniformity and stability of red blood cells.

Benefits of technology

The prepared red blood cell mimetic has good uniformity and stability, and is suitable for different types of urine analyzers, meeting the accuracy and reliability requirements of red blood cell detection in urine and reducing production costs.

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Abstract

The invention provides a red blood cell simulant as well as a preparation method and application thereof, and the preparation method comprises the following steps: (1) pretreatment: obtaining mammal whole blood and purifying to obtain red blood cells; (2) homogenization treatment: carrying out homogenization treatment on the red blood cells obtained in the step (1) by using a homogenization reagent; (3) cell repair treatment: carrying out cell repair treatment on the red blood cells obtained in the step (2) by using cell repair liquid; and (4) fixing treatment: fixing the red blood cells obtained in the step (3) by using a fixing agent. The preparation method disclosed by the invention is simple to operate and beneficial to large-scale production of products; the red blood cells in the prepared red blood cell simulant are good in homogeneity and stable in performance, the red blood cells in the red blood cell simulant are similar to red blood cells in human urine in shape, and the red blood cell simulant is used for preparing quality control substances for urine visible component analysis and can meet the requirements for accuracy and reliability of detection of the red blood cells in the urine.
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Citation Information

Cited By

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