Temperature difference variable micro-sampling device and method
Through the temperature difference-variable microsampling device, the volume changes of semiconductor storage tanks and thermal expansion medium are used to solve the problems of large structure and insufficient accuracy of microsampling devices in the prior art, and accurate microsampling and device volume reduction are achieved.
Patent Information
- Application Number
- CN202411343600.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-25
- Publication Date
- 2024-12-27
AI Technical Summary
The existing microsampling devices have huge structures, which are difficult to achieve accurate volume sampling control, and involve multiple mechanical power components.
The temperature difference-variable microsampling device is adopted to achieve volume changes through temperature control using semiconductor storage tanks and thermal expansion media, reducing mechanical power components, simplifying the structure and improving accuracy.
The microsampling accuracy is improved and the device volume is reduced, the device structure is simplified, and the sampling accuracy and efficiency are improved.
Smart Images
Figure CN119198201A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of sampling and detection, and in particular to a temperature difference-induced micro-sampling device and method. Background Art
[0002] At present, micro-sampling technology is widely used in the fields of medicine, life sciences, etc., and is one of the important processes for water sample testing. The existing micro-sampling technology mainly controls the displacement of the air cavity piston indirectly by controlling the compression of the spring, so as to suck the liquid into the air cavity generated by the movement of the piston to achieve sampling. However, the micro-sampling device using this method involves many parts, the overall volume of the device is relatively large, and it is difficult to achieve precise volume sampling control. Summary of the invention
[0003] In order to overcome the deficiencies of the prior art, the object of the present invention is to provide a temperature-induced micro-sampling device and method, which has the advantages of simplifying the device structure and improving the micro-sampling accuracy.
[0004] The purpose of the present invention is achieved by the following technical solutions: According to a first aspect of an embodiment of the present disclosure, a temperature-induced micro-sampling device is provided, comprising: A semiconductor storage tank for storing a heat expansion medium, wherein the semiconductor storage tank is capable of generating heat or absorbing heat to heat the heat expansion medium and is capable of forming an initial volume cavity just for storing the heat expansion medium; A sampling part, connected to the bottom of the semiconductor storage tank, used for feeding the sample liquid to be tested; A temperature detection module, used to detect the temperature of the thermal expansion medium; A control unit conductively connected to the semiconductor storage tank is used to control the semiconductor storage tank to heat up until the temperature of the thermal expansion medium reaches a predetermined temperature based on the thermal expansion coefficient of the thermal expansion medium according to the volume to be sampled, so that the thermal expansion medium produces a volume change corresponding to the volume to be sampled, and the volume change causes the corresponding volume of the sample liquid to be discharged from the sampling part to achieve micro sampling.
[0005] To implement the above technical solution, when sampling, the initial volume is first filled with a heat expansion medium. At this time, the temperature, thermal expansion coefficient and initial volume of the heat expansion medium are determined. The temperature of the heat expansion medium is collected by the temperature detection module. Since the volume change of the heat expansion medium changes linearly with the temperature, the predetermined heating temperature required to achieve the volume change of the volume to be sampled can be obtained according to the linear relationship. The semiconductor storage tank is powered on and heated by the control unit until the thermal expansion coefficient reaches the predetermined temperature and then stops. At this time, the heat expansion medium is restricted by the initial volume, and the volume change acts on the sampling part, and the corresponding volume of the sample liquid to be tested is discharged onto the test paper or the detection device, and the micro-sampling process can be completed. Since the sampling volume is controlled by the temperature difference variation method, the setting of the mechanical power components is reduced, the overall structure of the device is simplified, and the volume of the device can be reduced to a certain extent. Since the characteristics of the heat expansion medium are certain, the accuracy of the volume change is higher, thereby improving the micro-sampling accuracy.
[0006] In some exemplary embodiments, the semiconductor storage tank is made of a heat-conducting metal or a heat-conducting layer made of a heat-conducting metal is provided on the inner wall of the semiconductor storage tank to generate heat or absorb heat.
[0007] The implementation of the above technical solution enables the semiconductor storage tank to be heated directly or through the heat-conducting layer, and can also absorb external heat to heat the thermal expansion medium.
[0008] In some exemplary embodiments, a conductive sheet for electrically connecting to the control unit is disposed on the top of the semiconductor storage tank, and the control unit supplies electricity to the semiconductor storage tank or the heat-conducting layer through the conductive sheet to achieve heating.
[0009] The above technical solution is implemented to facilitate conductive connection with the control unit for heating control.
[0010] In some exemplary embodiments, the predetermined temperature is calculated according to the following formula: Δ𝑉=𝑉0×𝛼×Δ𝑇; T = T0 + Δ𝑇; Among them, Δ𝑉 is the volume to be sampled, 𝑉0 is the initial volume of the thermal expansion medium, 𝛼 is the thermal expansion coefficient of the thermal expansion medium, Δ𝑇 is the temperature change required to achieve the volume change, T0 is the initial temperature, and T is the predetermined temperature.
[0011] In some exemplary embodiments, a first piston is sealingly and slidably disposed in the semiconductor storage tank, and the first piston can remain in a stationary state to form the initial volume cavity between the first piston and the bottom of the semiconductor storage tank.
[0012] In some exemplary embodiments, the sampling portion is a sampling head, and the semiconductor tank stores a sample liquid to be tested that directly serves as a thermal expansion medium. The volume change generated by heating the sample liquid to be tested in the semiconductor tank is directly discharged from the sampling head to achieve micro-sampling.
[0013] In some exemplary embodiments, the first piston is connected to a first servo drive mechanism, and the first servo drive mechanism is used to drive the first piston to rise to inhale the sample liquid, or drive the first piston to descend to discharge the sample liquid, or keep the first piston in a stationary state.
[0014] To implement the above technical solution, before sampling, the first piston is located at the lowest position and fits with the bottom wall of the semiconductor storage tank, and the first servo drive mechanism drives the first piston to move upward into the semiconductor storage tank to draw a predetermined volume of the sample liquid to be tested and form an initial cavity; during the sampling process, the first servo drive mechanism controls the first piston to remain stationary so that the initial cavity remains fixed. When the sample liquid to be tested is heated, since the sample liquid to be tested is directly used as the thermal expansion medium, the volume change of the thermal expansion medium at this time can be directly converted to the sampling part, and the corresponding volume of the sample liquid to be tested is discharged, thereby completing the micro-sampling process; after the sampling is completed, the first servo drive mechanism drives the first piston downward to discharge the remaining sample liquid to be tested in the semiconductor storage tank so as to carry out the next sampling.
[0015] In some exemplary embodiments, a second piston is sealingly and slidingly disposed in the semiconductor storage tank, and a sealing plate is provided on the upper portion of the semiconductor storage tank. The second piston can remain stationary to form the initial cavity between the second piston and the sealing plate. The sampling portion is used to store the sample liquid to be tested. The volume change generated by the semiconductor storage tank heating the thermal expansion medium drives the second piston to move downward to discharge the sample liquid to be tested from the sampling portion to achieve micro-sampling.
[0016] In some exemplary embodiments, the sampling portion comprises: A sampling cylinder, used for storing the sample liquid to be tested, and a sampling dripper is provided at the bottom of the sampling cylinder; A third piston is sealingly and slidably connected to the sampling tube and connected to the first piston via a connecting rod; When the second piston moves downward, the connecting rod drives the third piston to move downward synchronously, so as to discharge the sample liquid to be tested from the sampling dripper to realize micro sampling.
[0017] In some exemplary embodiments, an electrically controlled locking clamp is further provided at the bottom of the semiconductor storage tank, and the electrically controlled locking clamp is used to clamp, lock or unlock the connecting rod.
[0018] In some exemplary embodiments, the sampling cylinder is connected to a second servo drive mechanism, which is used to drive the sampling cylinder to rise to inhale the sample liquid, or drive the sampling cylinder to descend to discharge the sample liquid, or keep the sampling cylinder in a stationary state.
[0019] To implement the above technical solution, before sampling, a predetermined volume of heat expansion medium is stored in the semiconductor storage tank, and the second piston is used to seal the tank to form an initial cavity. At this time, the third piston is located at the lowest position and fits the bottom wall of the sampling tube. The connecting rod is clamped and fixed by the electric lock clamp, and the sampling tube is driven downward by the second servo drive mechanism to draw a predetermined volume of the sample liquid to be tested into the sampling tube. During the sampling process, the electric lock clamp is first controlled to release the connecting rod so that it can move freely, and the sampling tube is controlled by the second servo drive mechanism to keep the sampling tube stationary. When the heat expansion medium is heated, the volume change of the heat expansion medium can drive the second piston to move downward, and then the third piston is driven to move downward synchronously through the connecting rod, so that the corresponding volume of the sample liquid to be tested is discharged from the sampling dripper, and the micro-sampling process can be completed. After the sampling is completed, the connecting rod is clamped and fixed by the electric lock clamp, and the sampling tube is driven upward by the second servo drive mechanism to discharge the remaining sample liquid to be tested in the sampling tube for the next sampling.
[0020] In some exemplary embodiments, the thermal expansion medium is argon, helium, neon, carbon dioxide or nitrogen.
[0021] According to a second aspect of an embodiment of the present disclosure, a temperature difference-induced micro-sampling method is provided, comprising: Obtaining the temperature and initial volume of the heat expansion medium currently stored in the initial volume of the semiconductor storage tank; According to the volume to be sampled, based on the thermal expansion coefficient of the thermal expansion medium, the semiconductor storage tank is controlled to heat up until the temperature of the thermal expansion medium reaches a predetermined temperature, so that the thermal expansion medium produces a volume change corresponding to the volume to be sampled, and the volume change causes the corresponding volume of the sample liquid to be discharged from the sampling part connected to the bottom of the semiconductor storage tank to achieve micro sampling.
[0022] To implement the above technical solution, the sampling volume is controlled by adopting a temperature difference-induced change method, which reduces the setting of mechanical power components and simplifies the overall structure of the device, thereby reducing the size of the device to a certain extent. Moreover, since the characteristics of the thermal expansion medium are constant, the volume change is more accurate, thereby improving the micro-sampling accuracy.
[0023] In some exemplary embodiments, the initial volumetric cavity stores the sample liquid directly serving as a thermal expansion medium, and the volume change generated by heating the sample liquid in the semiconductor storage tank is directly discharged from the sampling portion to achieve micro-sampling.
[0024] To implement the above technical solution, for the sample liquid that is not easy to volatilize or will not undergo other chemical reactions after heating, the method of directly heating the sample liquid to be tested is adopted to achieve sampling, so that the structure of the sampling device that realizes the corresponding function can be simpler.
[0025] In some exemplary embodiments, the sample liquid is stored in the sampling portion, and the volume change generated by the semiconductor storage tank heating the thermal expansion medium drives the sampling portion to expand and contract to discharge the sample liquid to achieve micro-sampling.
[0026] To implement the above technical solution, for the sample liquid to be tested that is volatile or prone to other chemical reactions after heating, the sampling part is used to convert the volume change to achieve the sampling process, thereby ensuring the stability of the sample liquid to be tested.
[0027] In summary, compared with the prior art, the present invention has the following beneficial effects: The embodiment of the present invention provides a temperature-dependent micro-sampling device and method. When sampling, a thermal expansion medium is first filled in an initial volume. At this time, the temperature, thermal expansion coefficient and initial volume of the thermal expansion medium are determined. The temperature of the thermal expansion medium is collected by a temperature detection module. Since the volume change of the thermal expansion medium changes linearly with the temperature, a predetermined heating temperature required to achieve the volume change of the volume to be sampled can be obtained according to the linear relationship. The semiconductor storage tank is powered on and heated by a control unit until the thermal expansion coefficient reaches the predetermined temperature and then stops. At this time, the thermal expansion medium is restricted by the initial volume, and the volume change acts on the sampling part, and the corresponding volume of the sample liquid to be tested is discharged onto the test paper or the detection device, and the micro-sampling process can be completed. Since the sampling volume is controlled by a temperature-dependent method, the setting of the mechanical power components is reduced, the overall structure of the device is simplified, and the volume of the device can be reduced to a certain extent. Since the characteristics of the thermal expansion medium are certain, the accuracy of the volume change is higher, thereby improving the micro-sampling accuracy. BRIEF DESCRIPTION OF THE DRAWINGS
[0028] Figure 1 It is a structural diagram of embodiment 1 of the present invention.
[0029] Figure 2 It is a structural diagram of embodiment 2 of the present invention.
[0030] Figure 3 This is a schematic diagram of the structure of the third embodiment of the present invention.
[0031] Figure 4 This is a structural diagram of embodiment 4 of the present invention.
[0032] The numbers and letters in the figure represent the corresponding component names: 10. Semiconductor storage tank; 11. Initial cavity; 12. Conductive sheet; 13. First piston; 14. Second piston; 15. Sealing plate; 20. Sampling part; 21. Sampling head; 22. Sampling tube; 23. Sampling dripper; 24. Third piston; 25. Connecting rod; 30. Temperature detection module; 40. Control unit; 50. First servo drive mechanism; 60. Electric lock clamp; 70. Second servo drive mechanism. DETAILED DESCRIPTION
[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0034] Embodiment 1 like Figure 1 As shown, an embodiment of the present invention provides a temperature-induced micro-sampling device, comprising: a semiconductor storage tank 10 for storing a thermal expansion medium, the semiconductor storage tank 10 can generate heat or absorb heat to heat the thermal expansion medium, and can form an initial volume 11 just for storing the thermal expansion medium; a sampling portion 20, connected to the bottom of the semiconductor storage tank 10, for feeding a sample liquid to be tested; a temperature detection module 30, for detecting the temperature of the thermal expansion medium; a control unit 40 conductively connected to the semiconductor storage tank 10, for controlling the semiconductor storage tank 10 to heat up to a predetermined temperature based on the thermal expansion coefficient of the thermal expansion medium according to the volume to be sampled, so that the thermal expansion medium generates a volume change corresponding to the volume to be sampled, and the volume change causes the corresponding volume of the sample liquid to be tested to be discharged from the sampling portion 20 to achieve micro-sampling.
[0035] Specifically, the semiconductor storage tank 10 is made of a heat-conducting metal or a heat-conducting layer made of a heat-conducting metal is arranged on the inner wall of the semiconductor storage tank 10 to generate heat or absorb heat. In this embodiment, the semiconductor storage tank 10 is integrally formed with a heat-conducting metal. In other embodiments, a heat-conducting layer made of a metal material may be closely arranged on the semiconductor storage tank 10, or a number of heating rods or heating plates may be arranged around the inner wall of the semiconductor storage tank 10. This is not limited in this embodiment as long as the heat expansion medium therein can be uniformly heated after power is turned on; during use, other auxiliary heating devices may be arranged outside the semiconductor, and heat may be absorbed and conducted through the semiconductor storage tank 10 or the heat-conducting layer to heat the heat expansion medium.
[0036] A conductive sheet 12 for electrically connecting to the control unit 40 is provided on the top of the semiconductor storage tank 10. The conductive sheet 12 can be connected to the semiconductor storage tank 10 by soldering or by clamping. The conductive sheet 12 is suitable for electrically connecting to a conductive wiring harness, for example, it can be configured to be in the shape of a conductive terminal. The control unit 40 energizes the semiconductor storage tank 10 or the heat-conducting layer through the conductive sheet 12 to achieve heating. The control unit 40 is mainly used to process temperature data and control whether to energize the semiconductor storage tank 10 according to the temperature data.
[0037] The control unit 40 may include, for example, a microcontroller unit (MCU), a central processing unit (CPU), a microprocessor (microprocessor), a digital signal processor (DSP), a programmable controller, a programmable logic device (PLD) or other similar devices or a combination of these devices, and the present invention is not limited thereto. In addition, in some embodiments, the functions of the control unit 40 may be implemented by multiple coding programs, which are stored in a storage medium and executed by the control unit 40. Alternatively, in some embodiments, the functions of the control unit 40 may be implemented by one or more circuits, and the present invention is not limited to the implementation of the functions of the control unit 40 by software or hardware.
[0038] A first piston 13 is sealingly and slidingly disposed in the semiconductor storage tank 10. The first piston 13 can remain stationary to form an initial volume 11 between the first piston 13 and the bottom of the semiconductor storage tank 10. A driving rod is connected to the upper end of the first piston 13 for connecting to an external power device to realize the lifting and lowering drive of the first piston 13. Of course, in some embodiments, in order to ensure that the volume of the sample liquid to be tested is sucked into the semiconductor storage tank 10 more accurately, a limit stopper can be set on the inner wall of the semiconductor storage tank 10. When the first piston 13 moves to conflict with the limit stopper, the corresponding lifted sample liquid to be tested can be sucked.
[0039] In this embodiment, the sampling portion 20 is a sampling head 21, which adopts a commercially available dripper with self-sealing properties, that is, under normal conditions, no liquid will leak from the sampling head 21, and the semiconductor tank 10 stores the sample liquid to be tested directly as a thermal expansion medium. The volume change generated by the semiconductor tank 10 after heating the sample liquid to be tested is directly discharged from the sampling head 21 to achieve micro-sampling. Of course, when this method is adopted, the sample liquid to be tested is not easy to volatilize or other chemical reactions will not occur when heated.
[0040] The temperature detection module 30 includes one or more temperature measuring elements, which may be, for example, temperature measuring resistors or temperature sensors. The temperature measuring elements are bonded and fixed to the bottom wall of the semiconductor storage tank 10 so as not to affect the downward movement of the first piston 13. For example, a groove may be provided on the bottom of the semiconductor storage tank 10 for the temperature measuring element to be embedded. The temperature measuring element may be connected to the control unit 40 via an insulating wiring harness arranged in the semiconductor storage tank 10. Of course, it may also be electrically connected to the control unit 40 by providing a conductive sheet 12.
[0041] When performing sampling control, the predetermined temperature needs to be calculated in advance, and the sample liquid can be directly heated to the predetermined temperature to expand to the corresponding volume. The predetermined temperature is calculated according to the following formula: Δ𝑉=𝑉0×𝛼×Δ𝑇; T = T0 + Δ𝑇; Among them, Δ𝑉 is the volume to be sampled, 𝑉0 is the initial volume of the thermal expansion medium, 𝛼 is the thermal expansion coefficient of the thermal expansion medium, Δ𝑇 is the temperature change required to achieve the volume change, T0 is the initial temperature, and T is the predetermined temperature.
[0042] Taking water as an example, the density of water is maximum at 4°C, and the density gradually decreases with the increase of temperature. The linear thermal expansion coefficient of water is approximately α≈0.00021 / °C; for example, before sampling begins, the initial temperature T0 of the thermal expansion medium (the sample liquid to be tested in this embodiment) collected by the temperature detection module 30 is 20°C, and the initial volume 𝑉0 of the thermal expansion medium stored in the initial cavity 11 is 5mL. If the volume to be sampled Δ𝑉 is 10μL, the temperature change Δ𝑇=Δ𝑉 / (𝑉0×𝛼). Substituting the above data into the calculation, the temperature change Δ𝑇 is 9.52°C, and the predetermined temperature is T0+Δ𝑇=29.52°C. During sampling, the control unit 40 controls the application of voltage to the semiconductor storage tank 10, and the semiconductor storage liquid begins to heat the thermal expansion medium until the temperature detection module 30 detects that the temperature reaches 29.52°C and stops heating. At this time, the thermal expansion medium can expand by 10μL and be squeezed out from the sampling head 21, and the micro-sampling operation can be completed.
[0043] When sampling the above-mentioned temperature-dependent micro-sampling device, first fill the initial cavity 11 with a heat expansion medium. At this time, the temperature, thermal expansion coefficient and initial volume of the heat expansion medium are determined. The temperature of the heat expansion medium is collected by the temperature detection module 30. Since the volume change of the heat expansion medium changes linearly with the temperature, the predetermined temperature required for heating to achieve the volume change of the volume to be sampled can be obtained according to the linear relationship. The semiconductor storage tank 10 is powered on and heated by the control unit 40 until the thermal expansion coefficient reaches the predetermined temperature and then stops. At this time, the heat expansion medium is restricted by the initial cavity 11, and the volume change acts on the sampling part 20, and the corresponding volume of the sample liquid to be tested is discharged onto the test paper or the detection device, and the micro-sampling process can be completed. Since the sampling volume is controlled by the temperature-dependent method, the setting of the mechanical power components is reduced, the overall structure of the device is simplified, and the device volume can be reduced to a certain extent. Since the characteristics of the heat expansion medium are certain, the accuracy of the volume change is higher, thereby improving the micro-sampling accuracy.
[0044] Embodiment 2 The difference between this embodiment and the first embodiment is that: in this embodiment, Figure 2 As shown, the first piston 13 is connected to a first servo drive mechanism 50, and the first servo drive mechanism 50 is used to drive the first piston 13 to rise to inhale the sample liquid to be tested, or drive the first piston 13 to descend to discharge the sample liquid to be tested, or keep the first piston 13 in a stationary state. The first servo drive mechanism 50 can, for example, adopt a servo electric cylinder, a servo screw mechanism, etc., and can maintain a self-locking state when the action stops. When its motor rotates forward or reverse, it can drive the first piston 13 to descend or move upward; it can be understood that, usually the semiconductor storage tank 10 and the first servo drive mechanism 50 are fixed on a frame to keep the two in a relatively static state.
[0045] Based on the setting of the first servo drive mechanism 50, before sampling, the first piston 13 is located at the lowest position and fits with the bottom wall of the semiconductor storage tank 10. The first servo drive mechanism 50 drives the first piston 13 to move upward into the semiconductor storage tank 10 to draw a predetermined volume of the sample liquid to be tested and form an initial cavity 11; during the sampling process, the first servo drive mechanism 50 controls the first piston 13 to remain stationary, so that the initial cavity 11 remains fixed. When the sample liquid to be tested is heated, since the sample liquid to be tested is directly used as the thermal expansion medium, the volume change of the thermal expansion medium at this time can be directly converted to the sampling part 20, and the corresponding volume of the sample liquid to be tested is discharged, and the micro-sampling process can be completed; after the sampling is completed, the first servo drive mechanism 50 drives the first piston 13 to move downward again to discharge the remaining sample liquid to be tested in the semiconductor storage tank 10 for the next sampling.
[0046] Embodiment 3 The difference between this embodiment and the first embodiment is that: in this embodiment, Figure 3 As shown, a second piston 14 is sealingly and slidingly provided in the semiconductor storage tank 10, and a sealing plate 15 is provided on the upper part of the semiconductor storage tank 10. The second piston 14 can remain stationary to form an initial cavity 11 between the second piston 14 and the sealing plate 15. The sampling portion 20 is used to store the sample liquid to be tested. The volume change generated by the semiconductor storage tank 10 heating the thermal expansion medium drives the second piston 14 to move downward to discharge the sample liquid to be tested from the sampling portion 20 to achieve micro-sampling.
[0047] Among them, in this embodiment, the temperature detection module 30 needs to be set at the sealing plate 15 so as not to affect the upward movement of the second piston 14. For example, a groove can be set on the sealing plate 15 for the temperature detection module 30 to be embedded; and in this embodiment, the thermal expansion medium preferably adopts a gas medium, such as a mixture of one or more of argon, helium, neon, carbon dioxide or nitrogen.
[0048] At the same time, a heat dissipation tube can be arranged outside the semiconductor storage tank 10, and a spiral heat dissipation channel is arranged between the heat dissipation tube and the semiconductor storage tank 10. The heat dissipation channel is connected to the coolant circulation device, and the heat expansion medium can be quickly cooled by the circulation of the coolant.
[0049] The sampling part 20 includes: a sampling cylinder 22, which is used to store the sample liquid to be tested, and a sampling dripper 23 is provided at the bottom of the sampling cylinder 22, and the sampling dripper 23 can also adopt an existing self-sealing dripper; a third piston 24 is sealingly and slidably connected to the sampling cylinder 22, and is connected to the first piston 13 through a connecting rod 25; when the second piston 14 moves downward, the third piston 24 is driven to move downward synchronously through the connecting rod 25 to discharge the sample liquid to be tested from the sampling dripper 23 to realize micro sampling.
[0050] In this embodiment, the semiconductor storage tank 10 and the sampling tube 22 are both connected to a frame. Before sampling, the heat expansion medium is kept in a high temperature state, and the third piston 24 is located at the bottom of the sampling tube 22. Then, the coolant circulation device is started to quickly cool the heat expansion medium. At this time, the heat expansion medium contracts. Under the action of external air pressure, the second piston 14 drives the third piston 24 to move upward, and the sample liquid to be tested can be sucked into the sampling tube 22 from the sampling dripper 23. When sampling, the semiconductor storage tank 10 is controlled again to heat the heat expansion medium. The heat expansion medium expands due to heat and changes in volume, thereby causing the second piston 14 and the third piston 24 to move downward, thereby squeezing out the sample liquid to be tested. When heated to a predetermined temperature, the corresponding volume of the sample liquid to be tested can be discharged, completing the micro-sampling process.
[0051] Embodiment 4 The difference between this embodiment and the third embodiment is that: in this embodiment, Figure 4As shown, an electric-controlled locking clamp 60 is also provided at the bottom of the semiconductor storage tank 10. The electric-controlled locking clamp 60 is used to clamp, lock or unlock the connecting rod 25. The electric-controlled locking clamp 60 can adopt an existing clamping device structure as long as it can clamp or release the connecting rod 25.
[0052] The sampling tube 22 is also connected to a second servo drive mechanism 70, which is used to drive the sampling tube 22 to rise to inhale the sample liquid to be tested, or drive the sampling tube 22 to descend to discharge the sample liquid to be tested, or keep the sampling tube 22 in a static state; the second servo drive mechanism 70 can also adopt a servo electric cylinder, a servo screw mechanism, etc., which can maintain a self-locking state when the action stops, and when its motor rotates forward or reverse, it can drive the sampling tube 22 to descend or move up; it can be understood that usually the semiconductor storage tank 10, the electric control locking clamp 60 and the second servo drive mechanism 70 are all fixed on a frame to maintain a relatively static state.
[0053] Based on the arrangement of the electric control lock clamp 60 and the second servo drive mechanism 70, before sampling, a predetermined volume of thermal expansion medium is stored in the semiconductor storage tank 10, and the second piston 14 is used to seal to form an initial volumetric cavity 11. At this time, the third piston 24 is located at the lowest position and fits the bottom wall of the sampling tube 22. The connecting rod 25 is clamped and fixed by the electric lock clamp, and the sampling tube 22 is driven downward by the second servo drive mechanism 70 to draw a predetermined volume of the sample liquid to be tested into the sampling tube 22. During the sampling process, the electric lock clamp is first controlled to loosen the connecting rod 25 so that it can move freely. The sampling tube 22 is controlled by the second servo drive mechanism 70 to remain stationary. When the heat expansion medium is heated, the volume change of the heat expansion medium can drive the second piston 14 to move downward, and then drive the third piston 24 to move downward synchronously through the connecting rod 25, so that the corresponding volume of the sample liquid to be tested is discharged from the sampling dripper 23, and the micro-sampling process can be completed; after the sampling is completed, the connecting rod 25 is clamped and fixed by the electric locking clamp, and the sampling tube 22 is driven by the second servo drive mechanism 70 to move upward, so that the remaining sample liquid to be tested in the sampling tube 22 can be discharged for the next sampling.
[0054] Embodiment 5 The embodiment of the present invention provides a temperature-induced micro-sampling method, which is implemented based on the temperature-induced micro-sampling device of the first or second embodiment, and includes: S100 , obtaining the temperature and initial volume of the heat expansion medium currently stored in the initial volume 11 of the semiconductor storage tank 10 .
[0055] Specifically, the volume of the thermal expansion medium is determined by the volume of the initial volume chamber 11, so the initial volume can be controlled by setting the initial volume chamber 11 of a certain volume. The temperature of the thermal expansion medium is measured by the temperature detection module 30 and transmitted to the control unit 40 for storage and calculation.
[0056] S200, controlling the semiconductor storage tank 10 to heat up until the temperature of the thermal expansion medium reaches a predetermined temperature based on the thermal expansion coefficient of the thermal expansion medium according to the volume to be sampled, so that the thermal expansion medium produces a volume change corresponding to the volume to be sampled, and the volume change causes the corresponding volume of the sample liquid to be tested to be discharged from the sampling part 20 connected to the bottom of the semiconductor storage tank 10 to achieve micro-sampling.
[0057] Specifically, when performing sampling control, the predetermined temperature needs to be calculated in advance, and the sample liquid can be directly heated to the predetermined temperature to expand to the corresponding volume. The predetermined temperature is calculated according to the following formula: Δ𝑉=𝑉0×𝛼×Δ𝑇; T = T0 + Δ𝑇; Among them, Δ𝑉 is the volume to be sampled, 𝑉0 is the initial volume of the thermal expansion medium, 𝛼 is the thermal expansion coefficient of the thermal expansion medium, Δ𝑇 is the temperature change required to achieve the volume change, T0 is the initial temperature, and T is the predetermined temperature.
[0058] By adopting the temperature difference-induced change method to control the sampling volume, the setting of mechanical power components is reduced, and the overall structure of the device is simplified, so that the volume of the device can be reduced to a certain extent. Moreover, since the characteristics of the thermal expansion medium are certain, the volume change is more accurate, thereby improving the micro-sampling accuracy.
[0059] In the present embodiment, for the test liquid that is not easy to volatilize or will not produce other chemical reactions after heating, the initial cavity 11 stores the test liquid that directly serves as the thermal expansion medium, and the semiconductor storage tank 10 directly discharges the volume change generated after the test liquid is heated from the sampling part 20 to achieve micro-sampling; the method of directly heating the test liquid to achieve sampling makes the structure of the sampling device that realizes the corresponding function simpler.
[0060] Embodiment 6 The difference between this embodiment and the fifth embodiment is that in this embodiment, for the sample liquid to be tested that is volatile or prone to other chemical reactions after heating, the sample liquid to be tested is stored in the sampling part 20, and the volume change generated by the semiconductor storage tank 10 heating the thermal expansion medium drives the sampling part 20 to produce a telescopic change to discharge the sample liquid to be tested to achieve micro-sampling; the sampling part 20 is used to convert the volume change to achieve the sampling process, thereby ensuring the stability of the sample liquid to be tested.
[0061] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which are equivalent modifications and improvements made to the above embodiments based on the essential technology of the present invention, and all of them belong to the protection scope of the present invention.
Claims
1. A temperature-variable micro-sampling device, characterized in that: include: A semiconductor storage tank for storing a heat expansion medium, wherein the semiconductor storage tank is capable of generating heat or absorbing heat to heat the heat expansion medium and is capable of forming an initial volume cavity just for storing the heat expansion medium; A sampling part, connected to the bottom of the semiconductor storage tank, used for feeding the sample liquid to be tested; A temperature detection module, used to detect the temperature of the thermal expansion medium; A control unit conductively connected to the semiconductor storage tank is used to control the semiconductor storage tank to heat up until the temperature of the thermal expansion medium reaches a predetermined temperature based on the thermal expansion coefficient of the thermal expansion medium according to the volume to be sampled, so that the thermal expansion medium produces a volume change corresponding to the volume to be sampled, and the volume change causes the corresponding volume of the sample liquid to be discharged from the sampling part to achieve micro sampling.
2. The temperature-variable micro-sampling device according to claim 1, characterized in that: The semiconductor storage tank is made of a heat-conducting metal or a heat-conducting layer made of a heat-conducting metal is arranged on the inner wall of the semiconductor storage tank to generate heat or absorb heat.
3. The temperature-variable micro-sampling device according to claim 2, characterized in that: A conductive sheet for electrically connecting to the control unit is disposed on the top of the semiconductor storage tank, and the control unit supplies electricity to the semiconductor storage tank or the heat-conducting layer through the conductive sheet to achieve heating.
4. The temperature-variable micro-sampling device according to claim 1, 2 or 3, characterized in that: The predetermined temperature is calculated according to the following formula: Δ𝑉=𝑉0×𝛼×Δ𝑇; T = T0 + Δ𝑇; Among them, Δ𝑉 is the volume to be sampled, 𝑉0 is the initial volume of the thermal expansion medium, 𝛼 is the thermal expansion coefficient of the thermal expansion medium, Δ𝑇 is the temperature change required to achieve the volume change, T0 is the initial temperature, and T is the predetermined temperature.
5. The temperature-variable micro-sampling device according to claim 4, characterized in that: A first piston is sealingly and slidably disposed in the semiconductor storage tank, and the first piston can remain in a stationary state to form the initial volume cavity between the first piston and the bottom of the semiconductor storage tank.
6. The temperature-variable micro-sampling device according to claim 5, characterized in that: The sampling part is a sampling head, and the semiconductor tank stores the sample liquid to be tested directly as a thermal expansion medium. The volume change generated by the semiconductor tank heating the sample liquid to be tested is directly discharged from the sampling head to achieve micro sampling.
7. The temperature-variable micro-sampling device according to claim 6, characterized in that: The first piston is connected to a first servo drive mechanism, and the first servo drive mechanism is used to drive the first piston to rise to inhale the sample liquid to be tested, or drive the first piston to descend to discharge the sample liquid to be tested, or keep the first piston in a stationary state.
8. The temperature-variable micro-sampling device according to claim 4, characterized in that: A second piston is sealingly and slidingly arranged in the semiconductor storage tank, and a sealing plate is arranged on the upper part of the semiconductor storage tank. The second piston can remain in a stationary state to form the initial cavity between the second piston and the sealing plate. The sampling part is used to store the sample liquid to be tested. The volume change generated by the semiconductor storage tank heating the thermal expansion medium drives the second piston to move downward to discharge the sample liquid to be tested from the sampling part to realize micro sampling.
9. The temperature-variable micro-sampling device according to claim 8, characterized in that: The sampling unit comprises: A sampling cylinder, used for storing the sample liquid to be tested, and a sampling dripper is provided at the bottom of the sampling cylinder; A third piston is sealingly and slidably connected to the sampling tube and connected to the first piston via a connecting rod; When the second piston moves downward, the connecting rod drives the third piston to move downward synchronously, so as to discharge the sample liquid to be tested from the sampling dripper to realize micro sampling.
10. The temperature-variable micro-sampling device according to claim 9, characterized in that: An electric-controlled locking clamp is also provided at the bottom of the semiconductor storage tank, and the electric-controlled locking clamp is used to clamp, lock or unlock the connecting rod.
11. The temperature-variable micro-sampling device according to claim 10, characterized in that: The sampling cylinder is connected to a second servo drive mechanism, and the second servo drive mechanism is used to drive the sampling cylinder to rise to inhale the sample liquid to be tested, or to drive the sampling cylinder to descend to discharge the sample liquid to be tested, or to keep the sampling cylinder in a stationary state.
12. The temperature-variable micro-sampling device according to claim 8, characterized in that: The thermal expansion medium is argon, helium, neon, carbon dioxide or nitrogen.
13. A temperature-induced micro-sampling method, characterized in that: include: Obtaining the temperature and initial volume of the heat expansion medium currently stored in the initial volume of the semiconductor storage tank; According to the volume to be sampled, based on the thermal expansion coefficient of the thermal expansion medium, the semiconductor storage tank is controlled to heat up until the temperature of the thermal expansion medium reaches a predetermined temperature, so that the thermal expansion medium produces a volume change corresponding to the volume to be sampled, and the volume change causes the corresponding volume of the sample liquid to be discharged from the sampling part connected to the bottom of the semiconductor storage tank to achieve micro sampling.
14. The temperature-induced micro-sampling method according to claim 13, characterized in that: The initial volume chamber stores the sample liquid to be tested directly as a thermal expansion medium, and the volume change generated by the semiconductor storage tank after heating the sample liquid to be tested is directly discharged from the sampling part to achieve micro sampling.
15. The temperature-induced micro-sampling method according to claim 13, characterized in that: The sample liquid to be tested is stored in the sampling part. The volume change generated by the semiconductor storage tank heating the thermal expansion medium drives the sampling part to produce expansion and contraction changes to discharge the sample liquid to be tested to achieve micro sampling.