Fluorescence spectrometer equipment, fluorescence spectrometer system and fluorescence spectrum detection method

Abstract

The invention relates to a fluorescence spectrophotometer device, a system and a fluorescence spectrum detection method, by means of the fluorescence spectrophotometer device, the detection method comprises the following steps; firstly, laser is emitted and reflected to the sample detection system through the fluorescence excitation system; then, the sample detection system transmits the sample information spectrum to a fluorescence detection system; secondly, the fluorescence detection system converts an optical signal into an electric signal and transmits the electric signal to the data acquisition and analysis system; thirdly, the data acquisition and analysis system performs spectrum processing; the device is reasonable in design, compact in structure and convenient to use.

Description

Technical Field

[0001] The invention relates to fluorescence spectrometer equipment, a system and a fluorescence spectrum detection method. Background Art

[0002] FCS fluorescence correlation spectroscopy (Fluorescence Correlation Spectroscopy) and its derivative technologies have been widely used in the fields of quantitative detection of molecular interactions, research on cell signal transduction mechanisms, focusing research on biomolecular denaturation, protein phase transition research, single molecule research, antibody screening, pathogen detection, disease marker detection, enzyme activity detection, fluorescent probe characteristic analysis, nanoparticle characteristic analysis, polymer analysis, microfluidic technology development, etc. It has a wide range of applications in research fields such as biomedicine, chemistry, photophysics, micro-nano science, etc.

[0003] However, existing tests require human intervention, which poses a hazard to testers. At the same time, they require a strictly closed sterile environment, and there is a problem of sample contamination.

[0004] In addition, the spectrometer needs to process light multiple times, and there is energy loss and interference during the transmission of light, which leads to reduced detection accuracy and energy consumption.

[0005] In addition, the existing sample testing is to place the glass slide horizontally, so the operation is extremely strict and requires controlling the amount of dripping. It needs to be pressed or rubbed back and forth to achieve the bonding of the glass slide. However, due to the tension of the liquid, the droplets are aggregated, resulting in uneven detection results, and even glass slide separation due to improper handling of the glass slide. How to maximize the effective detection area has become a technical problem that urgently needs to be solved. Summary of the invention

[0006] The technical problem to be solved by the present invention is generally to provide a fluorescence spectrometer device, a system and a fluorescence spectrum detection method.

[0007] To solve the above problems, the technical solution adopted by the present invention is:

[0008] In order to realize spectrum detection of samples, a fluorescence spectrum detection method is provided, with the aid of a fluorescence spectrometer device, the detection method comprises the following steps;

[0009] First, the laser is emitted through the fluorescence excitation system and reflected to the sample detection system; then, the sample detection system transmits the sample information spectrum to the fluorescence detection system; secondly, the fluorescence detection system converts the optical signal into an electrical signal and sends it to the data acquisition and analysis system; thirdly, the data acquisition and analysis system performs spectral processing.

[0010] In order to realize vertical detection, a fluorescence spectrum detection method is provided, with the aid of a fluorescence spectrometer device, the fluorescence spectrometer device has an objective lens; the objective lens has a vertical orientation and a horizontal orientation;

[0011] First, the sample slide is placed horizontally and tested; then the sample slide is placed vertically, and after the sample stays in the pressed sample slide for a set time, the objective lens is adjusted to a horizontal direction and tested.

[0012] As a further improvement of the above technical solution:

[0013] In order to ensure that the optical paths of the two tests are consistent, the optical path inside the fluorescence spectrometer device remains unchanged when the objective lens is in a vertical orientation state and a horizontal orientation state.

[0014] The sample slide adjustment steps are provided; the adjustment steps are as follows;

[0015] In order to realize automated detection and more accurate detection, S1, first, start the middle lifting head, so that the auxiliary support plate seat and the lower shovel plate enter the radial guide groove downward; then, place the sample slide loaded with the sample into the groove of the support carrier, and the front end of the lower shovel plate is located below the support carrier;

[0016] S2, rotating the sample glass slide to the detection station, and the detection device automatically adjusts the position so that the downward objective lens detects and samples the sample glass slide;

[0017] S3, the objective lens rises and leaves and becomes horizontal through the pitching of the aircraft head;

[0018] S4, first, drive the active push rod A, the sliding shoe A slides in the vertical slideway, so that the front hinge plate swings obliquely upward, and the lower shovel plate uses the inclination to swing the carrier upward in an oblique direction; then, the active push plate A pushes the slave baffle plate B, so that the guide rod B moves forward under the guidance of the guide sleeve B, thereby continuing to push the carrier to continue to swing upward around the hinge axis and contact with the front hinge plate and slide upward, and at the same time, after the slave baffle plate B moves forward to the set position, it drives the auxiliary support plate seat plate forward; secondly, under the action of the counter-support force provided by the active push plate A, drive the auxiliary push rod B, so that the slave rod B moves forward, so that the lower shovel plate enters the neutral position of the carrier, so that the front hinge plate is vertically attached to the back of the carrier, and the limit table supports the lower part of the carrier; thirdly, the sample slide is set to a stationary time, so that the sample flows down in the sample slide;

[0019] S5, manipulating the objective lens to perform multi-point sampling on the sample slide;

[0020] S6, reverse movement, the auxiliary push rod B drives the slave rod B to retract, the active push plate A retracts and moves a set distance and contacts the pull-back baffle, so that the guide rod B retreats under the guidance of the guide sleeve B, so that the carrier is tilted back to reset or tilt, waiting to be taken out.

[0021] The present invention realizes two molecular and nanoparticle analysis technologies, fluorescence autocorrelation and cross-correlation spectroscopy, which are suitable for industrial automated testing; the developed precision optical instrument realizes the alternating laser excitation (ALEX) single-molecule fluorescence resonance energy transfer analysis technology based on the built-in confocal optical path and the single-photon counting detector with nanosecond time resolution, and analyzes the dynamic structure and function mechanism of biological macromolecules with single-molecule resolution; the precision optical instrument developed by the present invention can realize the full-automatic and three-dimensional precise positioning of the confocal pinhole, and is equipped with a three-dimensional positioning electric sample stage, which can realize batch detection of samples. More accurate detection is achieved. No human intervention is required, which avoids harm to the detection personnel and avoids the problem of sample contamination. The energy loss of light in transmission is reduced, there is no interference, and the detection accuracy is reduced and the energy consumption is reduced. The uneven detection effect caused by liquid tension is avoided, multi-point sampling is realized, the detection is more accurate, and the glass slide separation due to improper glass slide handling will not occur, and the detection effective area is increased. The present invention has reasonable design, low cost, strong and durable, safe and reliable, simple operation, time-saving and labor-saving, money-saving, compact structure and convenient use. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the preferred structure of the fluorescence spectrometer device of the present invention.

[0023] Figure 2 It is a schematic diagram of the system structure of the present invention.

[0024] Figure 3 It is a schematic diagram of the frame base structure of the present invention.

[0025] Figure 4 It is a schematic diagram of the structure of the light transmitter of the present invention.

[0026] Figure 5 It is a schematic diagram of the structure of the supporting carrier of the present invention.

[0027] Figure 6 This is the schematic diagram of the spectrometer.

[0028] Among them: 1. Fluorescence excitation system; 2. Sample detection system; 3. Fluorescence detection system; 4. Data acquisition and analysis system; 5. Light emitter A; 6. Light emitter B; 7. Single-mode optical fiber; 8. Wavelength division multiplexer; 9. Beam collimator; 10. Sample slide; 11. Objective lens; 12. Excitation dichroic mirror; 13. Emission dichroic mirror; 14. Emission lens B; 15. Fiber optic adapter; 16. Emission light filter; 17. Emission lens A; 18. Multimode optical fiber; 19. Single-photon avalanche photodiode; 20. Fresnel lens; 21. Rotating disk assembly; 22. Detection device ; 23. Inspection station; 24. Rack base; 25. Pitch head; 26. Unloading platform; 27. Loading platform; 28. Support carrier; 29. ​​Limiting platform; 30. Radial guide groove; 31. Intermediate lifting head; 32. Active push rod A; 33. Active push plate A; 34. Guide sleeve B; 35. Auxiliary push rod B; 36. From baffle B; 37. From light rod B; 38. Auxiliary support plate seat; 39. Lower pad; 40. Front hinge plate; 41. Lower shovel plate; 42. Guide rod B; 43. Vertical slide; 44. Slide shoe A; 45. Slide shoe B; 46. Spacer pad; 47. Neutral gear. DETAILED DESCRIPTION

[0029] like Figure 1-6 As shown, the fluorescence spectrometer device of this embodiment, as a preference, comprises a fluorescence excitation system 1 for emitting light to a sample detection system 2;

[0030] The sample detection system 2 is used to detect the sample and collect information and feed it back to the fluorescence detection system 3;

[0031] Fluorescence detection system 3, collects feedback light information and converts it into electronic data;

[0032] The data acquisition and analysis system 4 processes the electronic data to complete the sample detection.

[0033] like Figure 1 As a preferred structure, in the fluorescence excitation system 1, a light emitter A5 and a light emitter B6 are provided; laser emission is performed to realize multi-spectrum detection, and of course, multiple spectra can also be used.

[0034] The optical transmitter A5 and the optical transmitter B6 are connected to a wavelength division multiplexer 8 through independent single-mode optical fibers 7 to realize laser compounding and forwarding; the wavelength division multiplexer 8 is connected to a beam collimator 9 through corresponding single-mode optical fibers 7 to realize accurate emission;

[0035] The sample detection system 2 includes an excitation dichroic mirror 12 corresponding to the beam collimator 9; an objective lens 11 is arranged on one side of the excitation dichroic mirror 12; the objective lens 11 corresponds to the sample slide 10; thereby achieving accurate detection.

[0036] The fluorescence detection system 3 includes an emission dichroic mirror 13 corresponding to the excitation dichroic mirror 12; the emission dichroic mirror 13 corresponds to emission light filters 16 respectively; the two emission light filters 16 correspond to emission lens B14 and emission lens A17 respectively;

[0037] The emitting lens B14 and the emitting lens A17 correspond to the optical fiber adapter 15 respectively;

[0038] The optical fiber adapter 15 is connected to the single-photon avalanche photodiode 19 through the corresponding multimode optical fiber 18, thereby realizing spectrum collection and filtering, thereby converting the optical signal into an electrical signal.

[0039] The single-photon avalanche photodiode 19 is connected to the data acquisition and analysis system 4 to implement data processing.

[0040] As a theoretical improvement, in order to reduce the loss of light energy and ensure the light energy consumption caused by light reflection in the channel and the resulting interference, a Fresnel lens 20 is provided at the input end of the wavelength division multiplexer 8 and / or the input end of the beam collimator 9, thereby greatly reducing the calibration of the device's emission direction and meeting multi-path detection.

[0041] like Figure 2-5 The fluorescence spectrometer system of this embodiment, which is partially improved, includes a rotating disk assembly 21 with a detection station 23, which can be driven by gear rotation; a detection device 22 is provided at the detection station 23 for spectral detection; the detection device 22 includes a frame base 24; the frame base 24 can have multiple degrees of freedom such as lifting and lowering and translation.

[0042] A pitch head 25 is provided on the frame base 24 at a pitch of 90 degrees to realize downward and forward detection, thereby ensuring the consistency of the entire optical path and ensuring that the detection result is not disturbed by the device structure.

[0043] A fluorescence spectrometer device is provided on the pitch head 25, which may be the above-mentioned structure or a conventional spectrometer.

[0044] The rotating disk assembly 21 includes a downloading platform 26 and an loading platform 27 which rotate synchronously and are arranged up and down;

[0045] A support carrier 28 is hingedly connected to the outer periphery of the unloading platform 26 to realize multi-station detection;

[0046] A limiting platform 29 is provided outside the supporting carrier 28 to support the lower end of the supporting carrier 28 that is swung to a vertical state, so as to maintain the vertical state.

[0047] A flat support station for placing a support carrier 28 in a horizontal state is provided on the upper platform 27 for testing;

[0048] A plurality of radial guide grooves 30 passing through corresponding flat support stations are distributed on the upper platform 27 to realize a through-setting, which is convenient for driving the auxiliary side push robot to realize the swing of the carrier.

[0049] The fluorescence spectrometer device may be a fluorescence spectrometer device on the market, or the above-mentioned device may be used.

[0050] As one of the innovative points, an intermediate lifting head 31 rotates at the center of the unloading platform 26 and the loading platform 27; an auxiliary side-pushing manipulator is provided on the intermediate lifting head 31; it is raised upward from the center to the outside, thereby cooperating with the objective lens to swing forward, thereby reducing the lifting distance of the objective lens.

[0051] In order to simplify the structure and achieve precise driving, the auxiliary side-pushing manipulator includes an active push rod A32 and a guide sleeve B34 horizontally arranged on the intermediate lifting head 31; the active push rod A32 and the guide sleeve B34 are oriented toward the detection device 22 at the detection station 23; compared with the shaft drive, it reduces the number of drives and the outer diameter of the turntable; compared with the swing push rod drive, it shortens the radial distance and reduces the dead point, so that the glass slide can be in a horizontal and vertical state, making full use of the intermediate space, and realizing multi-drive through a valve group with unloading to simplify the structure.

[0052] As a specific structural innovation, an active push plate A33 is provided on the active push rod A32 to realize linkage forward push and pull back;

[0053] A guide rod B42 is radially sleeved on the guide sleeve B34, preferably connected by a spring, so as to increase damping, so that the first stage drive swings in one direction as much as possible, avoiding linkage, so as to better pass the glass slide through 45 degrees;

[0054] The guide sleeve B34 and the active push rod A32 are arranged up and down, so that the top and the bottom are driven separately;

[0055] In order to increase the stroke, an auxiliary push rod B35 is provided on the guide rod B42, and a secondary baffle B36 is provided on the base of the auxiliary push rod B35; the auxiliary push rod B35 has a telescopic secondary light rod B37;

[0056] The active push plate A33 is used to push the rear end of the slave baffle B36 forward;

[0057] A gap 47 is provided at the hinge of the carrier 28 to pass through the lower shovel plate 41 to avoid damage; a chamfer is provided at the head of the carrier 28 to achieve better swing drive;

[0058] An L-shaped auxiliary support plate seat 38 is provided in the radial guide groove 30;

[0059] It realizes the support, reduces friction, and facilitates height adjustment. The vertical plate of the auxiliary support plate seat 38 has a perforation for passing the corresponding active push rod A32 and the slave rod B37 respectively;

[0060] In order to achieve flexible driving and avoid jamming, the active push rod A32 and the end of the slave rod B37 are respectively hinged with a sliding shoe A44 and a sliding shoe B45;

[0061] A front hinge plate 40 is provided on the front side of the vertical plate of the auxiliary support plate seat 38, on which a film can be added to reduce friction;

[0062] A vertical slideway 43 is provided on the back of the front hinge plate 40, and the slide shoe A44 and the slide shoe B45 slide up and down in the vertical slideway 43 respectively;

[0063] There are spacer pads 46 at the upper and lower ends of the vertical slide 43 to achieve position limiting and spacing adjustment. A lower pad 39 is provided at the turning point of the auxiliary support plate seat 38 to achieve distance adjustment. A lower shovel plate 41 is provided at the lower end of the front hinged plate 40.

[0064] A pull-back baffle corresponding to the active push plate A33 is provided on the guide rod B42 to realize the pull-back and provide the retraction support;

[0065] The lower shovel plate 41, the front hinge plate 40, the spacer pad 46, the lower pad 39, the vertical plate of the auxiliary support plate seat 38, the baffle plate B36, the active push plate A33, and the pull-back baffle are arranged in sequence from front to back.

[0066] In order to reduce the power, the two sides of the back of the supporting vehicle 28 are provided with counterweights, and the center of gravity of the supporting vehicle 28 is on the outside of the back of the supporting vehicle 28, so that the upright supporting vehicle 28 naturally leans back.

[0067] The fluorescence spectrum detection method of this embodiment, with the aid of a fluorescence spectrometer device, comprises the following steps:

[0068] First, the laser is emitted through the fluorescence excitation system 1 and reflected to the sample detection system 2; then, the sample detection system 2 transmits the sample information spectrum to the fluorescence detection system 3; secondly, the fluorescence detection system 3 converts the optical signal into an electrical signal and sends it to the data acquisition and analysis system 4; thirdly, the data acquisition and analysis system 4 performs spectral processing.

[0069] The spectral information is collected and reflected back to the fluorescence detection system 3.

[0070] When the laser is transmitted in the single-mode optical fiber 7 , the direction of the laser is adjusted by the Fresnel lens 20 and directed to the input end of the wavelength division multiplexer 8 and / or the input end of the beam collimator 9 .

[0071] The fluorescence spectrum detection method of this embodiment is based on a fluorescence spectrometer device, and the fluorescence spectrometer device has an objective lens 11; the objective lens 11 has a vertical orientation and a horizontal orientation;

[0072] First, the sample slide 10 is placed horizontally and tested; then the sample slide 10 is placed vertically, and after the sample stays in the pressed sample slide 10 for a set time, the objective lens 11 is adjusted to a horizontal direction and tested.

[0073] When the objective lens 11 is in a vertical orientation state and a horizontal orientation state, the internal optical path of the fluorescence spectrometer device remains unchanged.

[0074] The objective lens 11 is not limited to the above-mentioned spectrometer, but may also be an objective lens of other conventional spectrometers.

[0075] As an example, in Figure 6 In the figure, (A) a confocal microscope is used to separate a very small fluorescence excitation and detection volume in the solution sample using the optical path setting. (B) Schematic diagram of the fluorescence excitation and detection volume. (C) Autocorrelation analysis (bottom) is performed on the fluorescence signal F(t) (top in the figure) that fluctuates over time. (D) The autocorrelation amplitude G(τ) decays as the correlation time τ increases, resulting in the autocorrelation decay curve shown in the figure (black experimental data points). (E) Fluorescence correlation spectrometer is used to detect the concentration of the fluorescent molecule Rhodamine 6G in a 70% sucrose solution. The molecular concentrations corresponding to the green autocorrelation decay curves (from top to bottom) are: 0.62, 1.25, 2.5, 5, 10 and 20 nanomoles (nM). Under high-intensity laser irradiation (red autocorrelation decay curve; 20nM Rhodamine 6G solution), Rhodamine 6G molecules undergo an electronic orbital transition from the Singlet State (fluorescence) to the Triplet State (non-fluorescence); this photophysical process is reflected in the additional autocorrelation decay amplitude in a shorter τ time period (<0.1ms). The number of Rhodamine 6G molecules in the fluorescence detection volume V can be inferred by N=1 / G(0). The number of molecules obtained in this way has a perfect linear relationship with the corresponding molecular concentration (see the inset in the upper right corner). (F) Fluorescence correlation spectrometer is used to detect the size of nanoparticles. For gold particles (Colloid GoldParticles) and latex beads (Latex Beads), autocorrelation analysis is performed on the backscattering (Back Scattering) laser signal that fluctuates with time; for quantum dots (Quantums Dots) and fluorescent beads (Fluorescence Beads), autocorrelation analysis is performed on the fluorescence signal that fluctuates with time.

[0076] The sample slide 10 is provided with adjustment steps; the adjustment steps are as follows;

[0077] S1, first, start the intermediate lifting head 31, so that the auxiliary support plate seat 38 and the lower shovel plate 41 enter the radial guide groove 30 downward; then, place the sample slide 10 loaded with the sample into the groove of the support carrier 28, and the front end of the lower shovel plate 41 is located below the support carrier 28;

[0078] S2, rotating the sample slide 10 to the detection station 23, and the detection device 22 automatically adjusts the position so that the downward objective lens 11 detects and samples the sample slide 10;

[0079] S3, the objective lens 11 rises and leaves and becomes horizontal through the pitch head 25;

[0080] S4, first, drive the active push rod A32, the sliding shoe A44 slides in the vertical slide 43, so that the front hinge plate 40 swings upward obliquely, and the lower shovel plate 41 uses the slope to swing the carrier 28 upward obliquely; then, the active push plate A33 pushes the baffle plate B36, so that the guide rod B42 moves forward under the guidance of the guide sleeve B34, thereby continuing to push the carrier 28 to continue to swing upward around the hinge axis and contact with the front hinge plate 40 and slide upward, and at the same time, the baffle plate B36 moves forward. After reaching the set position, the auxiliary support plate seat 38 is driven to move forward; secondly, under the action of the counter-support force provided by the active push plate A33, the auxiliary push rod B35 is driven to move forward from the light rod B37, so that the lower shovel plate 41 enters the gap 47 of the support carrier 28, so that the front hinge plate 40 is vertically attached to the back of the support carrier 28, and the limit table 29 supports the lower part of the support carrier 28; thirdly, the sample slide 10 is stationary for a set time, so that the sample flows down in the sample slide 10;

[0081] S5, manipulating the objective lens 11 to perform multi-point sampling on the sample slide 10;

[0082] S6, reverse movement, the auxiliary push rod B35 drives the slave rod B37 to retract, the active push plate A33 retracts and moves a set distance and contacts the pull-back baffle, so that the guide rod B42 retreats under the guidance of the guide sleeve B34, so that the support carrier 28 leans back to reset or tilt, waiting to be taken out.

[0083] In S1 , the sample slide 10 is sucked in the groove of the carrier 28 under negative pressure.

[0084] The present invention is fully described for a clearer disclosure, and the prior art is not listed one by one.

[0085] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some of the technical features therein with equivalents; it is obvious for those skilled in the art to combine multiple technical solutions of the present invention. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention. The technical contents not described in detail in the present invention are all known technologies.

Claims

1. A fluorescence spectrum detection method, characterized in that: With the aid of a fluorescence spectrometer device, the detection method comprises the following steps; First, the laser is emitted through the fluorescence excitation system (1) and reflected to the sample detection system (2); then, the sample detection system (2) transmits the sample information spectrum to the fluorescence detection system (3); secondly, the fluorescence detection system (3) converts the optical signal into an electrical signal and transmits it to the data acquisition and analysis system (4); thirdly, the data acquisition and analysis system (4) performs spectral processing. The spectral information is collected and reflected back to the fluorescence detection system (3).

2. The fluorescence spectrum detection method according to claim 1, characterized in that: When the laser is transmitted in the single-mode optical fiber (7), the direction of the laser is adjusted by the Fresnel lens (20) and directed to the input end of the wavelength division multiplexer (8) and / or the input end of the beam collimator (9).

3. A fluorescence spectrum detection method, characterized in that: By means of a fluorescence spectrometer device, the fluorescence spectrometer device has an objective lens (11); the objective lens (11) has a vertical orientation and a horizontal orientation; First, the sample glass slide (10) is placed horizontally and tested; then, the sample glass slide (10) is placed vertically, and after the sample stays in the pressed sample glass slide (10) for a set time, the objective lens (11) is adjusted to a horizontal orientation and tested.

4. The fluorescence spectrum detection method according to claim 3, characterized in that: When the objective lens (11) is in a vertical orientation state and a horizontal orientation state, the internal optical path of the fluorescence spectrometer device remains unchanged.

5. The fluorescence spectrum detection method according to claim 3, characterized in that: The sample slide (10) is provided with adjustment steps; the adjustment steps are as follows; S1, first, start the intermediate lifting head (31), so that the auxiliary support plate seat (38) and the lower shovel plate (41) enter the radial guide groove (30) downward; then, place the sample glass slide (10) loaded with the sample into the groove of the support carrier (28), and the front end of the lower shovel plate (41) is located below the support carrier (28); S2, rotating the sample glass slide (10) to the detection station (23), and the detection device (22) automatically adjusts the position so that the downward-facing objective lens (11) detects and samples the sample glass slide (10); S3, the objective lens (11) rises and leaves and becomes horizontal through the pitching head (25); S4, first, drive the active push rod A (32), the sliding shoe A (44) slides in the vertical slideway (43), so that the front hinge plate (40) swings upward obliquely, and the lower shovel plate (41) uses the inclination to swing the carrier (28) upward obliquely; then, the active push plate A (33) pushes the baffle plate B (36), so that the guide rod B (42) moves forward under the guidance of the guide sleeve B (34), thereby continuing to push the carrier (28) to continue to swing upward around the hinge axis and contact with the front hinge plate (40) and slide upward, and at the same time, the baffle plate B (36) moves forward. After reaching the set position, the auxiliary support plate seat (38) is driven to move forward; secondly, under the action of the counter-support force provided by the active push plate A (33), the auxiliary push rod B (35) is driven to move forward from the light rod B (37), so that the lower shovel plate (41) enters the neutral position (47) of the support carrier (28), so that the front hinge plate (40) is vertically attached to the back of the support carrier (28), and the limit table (29) supports the lower part of the support carrier (28); thirdly, the stationary sample slide (10) is set for a time, so that the sample flows down in the sample slide (10); S5, manipulating the objective lens (11) to perform multi-point sampling on the sample slide (10); S6, reverse movement, the auxiliary push rod B (35) drives the slave rod B (37) to retract, the active push plate A (33) retracts and moves a set distance and contacts the pull-back baffle, so that the guide rod B (42) is guided backward by the guide sleeve B (34), so that the support carrier (28) is tilted back to reset or tilt, waiting to be taken out.

6. The fluorescence spectrum detection method according to claim 3, characterized in that: In S1, the sample slide (10) is adsorbed under negative pressure in the groove of the carrier (28).

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