Intelligent extraction and analysis method for anti-aging collagen extract

Abstract

The invention belongs to the technical field of extraction and analysis of anti-aging collagen extracts, and particularly discloses and provides an intelligent extraction and analysis method for an anti-aging collagen extract, which comprises the following steps: extracting physical property information, chemical property information and stability information of the anti-aging collagen extract; the physical property qualification index, the chemical property qualification index and the stability qualification index of the anti-aging collagen extract are correspondingly analyzed, and then the quality qualification index of the anti-aging collagen extract is analyzed, so that corresponding feedback is performed; according to the method, comprehensive quality qualification analysis is carried out on the physical property, the chemical property and the stability of the anti-aging collagen extract, limitation of single-dimensional analysis is avoided, and meanwhile, the anti-aging collagen extract is obtained by extracting the appearance information, the solubility information and the viscosity information of the anti-aging collagen extract. The physical property qualification index of the anti-aging collagen extract is analyzed, and the using effect of the anti-aging collagen extract is guaranteed.

Description

Technical Field

[0001] The invention belongs to the technical field of extraction and analysis of anti-aging collagen extracts, and relates to an intelligent extraction and analysis method for anti-aging collagen extracts. Background Art

[0002] Anti-aging collagen extract is a substance separated and purified from collagen-rich animal tissues through intelligent extraction technology. It has biological activities such as anti-oxidation, promoting collagen synthesis, inhibiting collagen degradation, and promoting cell proliferation and differentiation. It can be used in cosmetics, food, and medicine to help delay skin aging and maintain the health of body tissues. Therefore, it is necessary to extract and analyze anti-aging collagen extracts.

[0003] The current extraction and analysis of anti-aging collagen extracts mainly focuses on their chemical properties, and there are still the following deficiencies: 1. The current extraction and analysis of anti-aging collagen extracts mainly focuses on the chemical property dimension, that is, insufficient attention is paid to the physical properties and stability of anti-aging collagen extracts, and thus the comprehensiveness of the quality assessment of anti-aging collagen extracts cannot be guaranteed, and thus their performance in actual applications cannot be accurately reflected.

[0004] 2. Currently, when analyzing the physical properties of anti-aging collagen extracts, the main consideration is the appearance information of the anti-aging collagen extracts, that is, insufficient attention is paid to its solubility and viscosity, which makes it impossible to guarantee the effectiveness of the anti-aging collagen extracts. At the same time, potential problems of the anti-aging collagen extracts cannot be discovered in time, thus affecting the safety of the anti-aging collagen extracts. Summary of the invention

[0005] In view of this, in order to solve the problems raised in the above background technology, an intelligent extraction and analysis method for anti-aging collagen extract is proposed.

[0006] The purpose of the present invention can be achieved through the following technical solutions: The present invention provides an intelligent extraction and analysis method for anti-aging collagen extracts, the method comprising: step one, extract information collection: randomly extracting a set number of anti-aging collagen extracts as target extracts, and collecting parameter information of the target extracts.

[0007] Step 2: Extraction of physical properties of the extract: Extracting the physical property information of the target extract, which includes appearance information, solubility information and viscosity information.

[0008] Step 3: Analysis of physical properties of the extract: Analyze the physical property qualification index of the target extract based on the physical property information of the target extract.

[0009] Step 4: Extraction of chemical properties of the extract: Extract the chemical property information of the target extract, which includes protein information and amino acid information.

[0010] Step 5: Chemical property analysis of the extract: Analyze the chemical property qualification index of the target extract based on the chemical property information of the target extract.

[0011] Step 6. Extract stability extraction: Extract the stability information of the target extract, which includes temperature stability information and light stability information.

[0012] Step 7. Extract stability analysis: Analyze the stability qualification index of the target extract based on the stability information of the target extract.

[0013] Step 8. Extract quality qualification analysis: Analyze the quality qualification index of the target extract based on the physical property qualification index, chemical property qualification index and stability qualification index of the target extract.

[0014] Step 9. Feedback on extract quality: Provide corresponding feedback based on the quality qualification index of the target extract.

[0015] Preferably, the physical property qualification index of the target extract is analyzed, including: A1, extracting the viscosity of each extract from the viscosity information of the target extract, denoted as η i , i is the extract number, i=1,2,......,m.

[0016] A2. Extracting the ideal viscosity range of the target polymer from the parameter information of the target extract, and recording the middle value of the ideal viscosity range of the target polymer as η′.

[0017] A3. Calculate the viscosity qualification index β of each extract i , Δη is the viscosity difference of the set reference.

[0018] A4. i The mean value is calculated and the calculated result is used as the viscosity qualification index of the target extract, which is recorded as

[0019] A5. According to the appearance information of the target extract, calculate the appearance qualification index of the target extract

[0020] A6. According to the solubility information of the target extract, calculate the solubility qualification index of the target extract

[0021] A7. Statistical evaluation of the physical property qualification index l1 of the target extract. z1, z2 and z3 are the weights of the set viscosity qualified index, appearance qualified index and solubility qualified index respectively, z1+z2+z3=1, z1>z2>z3.

[0022] Preferably, the statistically calculating the appearance qualification index of the target extract comprises: extracting the color of each extract from the appearance information of the target extract, and extracting the ideal color range of the target extract from the parameter information of the target extract.

[0023] The color of each extract is matched with the ideal color range of the target extract. If the color of an extract is within the ideal color range, the extract is recorded as a color-qualified extract. The number of color-qualified extracts is counted and recorded as D1.

[0024] The number of extracts is counted, recorded as D2, and the ratio of D1 to D2 is used as the color qualification index of the target extract, recorded as ε1.

[0025] The diameter of each powder in each extract was extracted from the appearance information of the target extract, and then the average diameter and standard diameter difference of each extract were calculated and recorded as γ i and λ i .

[0026] The coefficient of variation CV of each extract was calculated i ,

[0027] The coefficient of variation of each extract is compared with the coefficient of variation of the set reference. If the coefficient of variation of an extract is less than or equal to the coefficient of variation of the set reference, the extract is recorded as a qualified diameter extract. The number of qualified diameter extracts is counted, and the ratio of the qualified diameter extract to the number of extracts is used as the particle size qualification index of the target extract, recorded as ε2.

[0028] Statistical Appearance Qualification Index of Target Extract

[0029] Preferably, the statistical solubility qualification index of the target extract comprises: extracting the dissolution time and solubility of each extract after adding a set temperature and a set volume of solvent from the solubility information of the target extract, and recording them as T i and S i .

[0030] The ideal dissolution time and ideal solubility of the target extract are extracted from the parameter information of the target extract and are denoted as T′ and S′, respectively.

[0031] Statistical solubility qualification index of target extract ΔT and ΔS are the dissolution time difference and solubility difference of the set reference, respectively, and m is the number of extracts.

[0032] Preferably, the analysis of the chemical property qualification index of the target extract includes: extracting the protein content of each extract from the protein information of the target extract, and extracting the ideal protein content of the target extract from the parameter information of the target extract.

[0033] The protein content of each extract was compared with the ideal protein content. If the protein content of an extract was greater than or equal to the ideal protein content, the extract was recorded as an extract with qualified protein content, and the number of extracts with qualified protein content was counted.

[0034] The ratio of the number of extracts with qualified protein content to the number of extracts was taken as the qualified protein content index of the target polymer, denoted as

[0035] According to the amino acid information of the target extract, the amino acid qualification index of the target polymer is calculated

[0036] Statistical target extract chemical property qualified index l2,

[0037] Preferably, the statistical amino acid qualification index of the target polymer includes: extracting the content of each amino acid in each extract from the amino acid information of the target extract.

[0038] The ratio of the content of each amino acid to the total content of amino acids was taken as the amino acid composition ratio of each extract.

[0039] The amino acid composition ratio of each extract was compared with the corresponding amino acid composition ratio range of the reference.

[0040] If the amino acid composition ratios of an extract are all within the range of the corresponding reference amino acid composition ratios, the extract is recorded as an extract with qualified amino acid content, the number of extracts with qualified amino acid content is counted, and the ratio of the qualified amino acid content to the number of extracts is used as the qualified amino acid composition index of the target polymer, recorded as

[0041] Preferably, the analysis of the stability qualification index of the target extract includes: B1, calculating the temperature stability qualification index ψ1 of the target extract according to the temperature stability information of the target extract.

[0042] B2. According to the photostability information of the target extract, the photostability qualified index ψ2 of the target extract is calculated.

[0043] B3. Statistical stability qualification index l3 of target extract, ψ1′ and ψ2′ are the temperature stability qualified index and light stability qualified index of the set reference respectively, and e is a natural constant.

[0044] Preferably, the statistical temperature stability qualified index of the target extract includes: extracting the appearance information, solubility information and viscosity information of each extract at each set temperature from the temperature stability information of the target extract, and then analyzing in the same way as the analysis method of the physical property qualified index of the target extract to obtain the physical property qualified index of the target extract at each set temperature.

[0045] With the set temperature as the horizontal axis and the physical property qualified index as the vertical axis, a physical property qualified index change curve of the target extract is constructed.

[0046] A reference change curve of a qualified index of physical properties of the target extract is extracted from the parameter information of the target extract.

[0047] The length of the curve segment below the reference variation curve of the physical property qualified index of the target extract is extracted and recorded as L1, and the length of the variation curve of the physical property qualified index of the target extract is recorded as L2.

[0048] The temperature stability qualified index ψ1 of the target extract is calculated.

[0049] Preferably, the statistical photostability qualified index of the target extract comprises: extracting the active ingredient content of each extract at each monitoring point under each set light intensity from the photostability information of the target extract, and then calculating the mean value thereof to obtain the average active ingredient content of each extract, which is recorded as ξ i , and at the same time extract the maximum active ingredient content and the minimum active ingredient content of each extract.

[0050] The maximum active ingredient content and the minimum active ingredient content of each extract were subtracted to obtain the fluctuation range of the active ingredient content of each extract, which was recorded as ξ i ′.

[0051] Statistical analysis of the qualified index σ of the active ingredient content of each extract i , ξ″ and ξ″′ are the active ingredient content and the fluctuation range of the active ingredient content of the set reference, respectively.

[0052] σ i The mean value is calculated and the calculated result is used as the qualified index of the change in the active ingredient content of the target extract, which is recorded as α1.

[0053] The color data of the target extract is extracted from the light stability information of the target extract, and then the color change qualified index α2 of the target extract is calculated.

[0054] The minimum value was selected from α1 and α2 as the photostability qualified index of the target extract and recorded as ψ2.

[0055] Preferably, the quality qualified index of the analyzed target extract includes: a qualified index l1 of the physical properties of the target extract, a qualified index l2 of the chemical properties of the target extract and a qualified index l3 of the stability of the target extract.

[0056] The quality qualified index θ of the target extract is calculated, θ=l1*q1+l2*q2+l3*q3, q1, q2 and q3 are the weights of the set physical property qualified index, chemical property qualified index and stability qualified index respectively, q1+q2+q3=1, q3>q2>q1.

[0057] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The present invention avoids the current problem of insufficient attention to the physical properties and stability of anti-aging collagen extracts by conducting a comprehensive quality assessment of the physical properties, chemical properties and stability of the anti-aging collagen extracts, while avoiding the limitations of single-dimensional analysis, thereby improving the comprehensiveness of the quality assessment of the anti-aging collagen extracts.

[0058] (2) The present invention extracts the appearance information, solubility information and viscosity information of the anti-aging collagen extract, analyzes the physical property qualification index of the anti-aging collagen extract, thereby breaking the current problem of insufficient attention to its solubility and viscosity, thereby ensuring the use effect of the anti-aging collagen extract, and at the same time improving the timeliness of discovering potential problems of the anti-aging collagen extract, thereby improving the safety of the anti-aging collagen extract.

[0059] (3) The present invention ensures the representativeness of the anti-aging collagen extract by randomly selecting a set number of anti-aging collagen extracts as target extracts, thereby improving the comprehensiveness of the reflection of the overall batch quality.

[0060] (4) When analyzing the qualified index of physical properties of anti-aging collagen extract, the present invention increases the importance of key properties by setting weights for the qualified index of viscosity, qualified index of appearance and qualified index of solubility, thereby improving the pertinence of the physical property analysis. At the same time, by setting weights, the accuracy of the physical property analysis results is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0061] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0062] Figure 1 It is a schematic diagram of the connection of each step of the method of the present invention.

[0063] Figure 2 It is a schematic diagram of the connection steps of the physical property qualification index analysis step of the present invention.

[0064] Figure 3 It is a schematic diagram of the connection steps of the stability qualification index analysis step of the present invention. DETAILED DESCRIPTION

[0065] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0066] See also Figure 1 As shown, the present invention provides an intelligent extraction and analysis method for anti-aging collagen extracts, the method comprising: step one, extract information collection: randomly extracting a set number of anti-aging collagen extracts as target extracts, and collecting parameter information of the target extracts.

[0067] It should be added that the parameter information of the target extract includes: the ideal viscosity range, ideal color range, ideal dissolution time, ideal solubility, ideal protein content and reference change curve of the qualified index of physical properties of the target polymer.

[0068] The embodiment of the present invention ensures the representativeness of the anti-aging collagen extract by randomly selecting a set number of anti-aging collagen extracts as target extracts, thereby improving the comprehensiveness of the reflection of the overall batch quality.

[0069] Step 2: Extraction of physical properties of the extract: Extracting the physical property information of the target extract, which includes appearance information, solubility information and viscosity information.

[0070] It should be supplemented that the appearance information of the target extract includes: the color of each extract and the diameter of each powder in each extract, the solubility information of the target extract includes: the dissolution time and solubility of each extract after adding a set temperature and a set volume of solvent, and the viscosity information of the target extract includes: the viscosity of each extract.

[0071] It should be added that the color of each extract is obtained by monitoring with a colorimeter, and the diameter of each powder in each extract is obtained in the following way: for approximately spherical particles, their diameter can be directly measured to indicate the particle size. For example, the diameter of a single spherical particle can be measured by combining a microscope with image analysis software. For non-spherical particles, it is difficult to directly use a diameter to accurately describe their size. In this case, an equivalent diameter can be used. For example, the equivalent volume diameter is the diameter of a spherical particle with the same volume as a non-spherical particle. The volume of the particle is measured and then the diameter of a spherical particle with the same volume is calculated as the equivalent diameter.

[0072] It should be added that the method for monitoring the dissolution time of each extract after adding a solvent of a set temperature and a set volume is as follows: after the extract is added to the solvent, a turbidity meter is used to monitor the turbidity changes of the solution in real time. As the extract dissolves, the turbidity of the solution will gradually decrease. When the turbidity reaches a stable low value, the extract can be considered to be completely dissolved. The time from the beginning to this state is recorded as the dissolution time. The solubility of each extract after adding a solvent of a set temperature and a set volume is obtained by monitoring and analyzing with a high performance liquid chromatograph.

[0073] It should also be added that the viscosity of each extract was monitored by a rotational viscometer method.

[0074] Step 3: Analysis of physical properties of the extract: Analyze the physical property qualification index of the target extract based on the physical property information of the target extract.

[0075] It should be added that the anti-aging collagen extract is in the form of powder, flake or solution. When performing extraction analysis in the present invention, the selected form is powder.

[0076] See also Figure 2 As shown, illustratively, the physical property qualification index of the target extract is analyzed, including: A1, extracting the viscosity of each extract from the viscosity information of the target extract, denoted as η i , i is the extract number, i=1,2,......,m.

[0077] A2. Extracting the ideal viscosity range of the target polymer from the parameter information of the target extract, and recording the middle value of the ideal viscosity range of the target polymer as η′.

[0078] A3. Calculate the viscosity qualification index β of each extract i , Δη is the viscosity difference of the set reference.

[0079] It should be added that Δη was extracted from the extraction and analysis technical specifications of the anti-aging collagen extract.

[0080] A4. i The mean value is calculated and the calculated result is used as the viscosity qualification index of the target extract, which is recorded as

[0081] A5. According to the appearance information of the target extract, calculate the appearance qualification index of the target extract

[0082] Furthermore, the statistical appearance qualification index of the target extract includes: A5-1, extracting the color of each extract from the appearance information of the target extract, and extracting the ideal color range of the target extract from the parameter information of the target extract.

[0083] A5-2. Match the color of each extract with the ideal color range of the target extract. If the color of an extract is within the ideal color range, the extract is recorded as a color-qualified extract. The number of color-qualified extracts is counted and recorded as D1.

[0084] A5-3. Count the number of extracts, record it as D2, and take the ratio of D1 to D2 as the color qualification index of the target extract, record it as ε1.

[0085] A5-4. Extract the diameter of each powder in each extract from the appearance information of the target extract, and then calculate the average diameter and standard diameter difference of each extract, and record them as γ i and λ i .

[0086] A5-5. Calculate the coefficient of variation (CV) of each extract i ,

[0087] A5-6. Compare the coefficient of variation of each extract with the coefficient of variation of the set reference. If the coefficient of variation of an extract is less than or equal to the coefficient of variation of the set reference, the extract is recorded as a diameter-qualified extract. The number of extracts with qualified diameters is counted, and the ratio of the coefficient of variation to the number of extracts is used as the particle size qualification index of the target extract, recorded as ε2.

[0088] A5-7. Calculate the appearance qualification index of the target extract

[0089] A6. According to the solubility information of the target extract, calculate the solubility qualification index of the target extract

[0090] Furthermore, the statistical solubility qualification index of the target extract includes: A6-1, extracting the dissolution time and solubility of each extract after adding a set temperature and a set volume of solvent from the solubility information of the target extract, respectively recorded as T iand S i .

[0091] A6-2. Extract the ideal dissolution time and ideal solubility of the target extract from the parameter information of the target extract, and record them as T′ and S′ respectively.

[0092] A6-3. Calculate the solubility index of the target extract ΔT and ΔS are the dissolution time difference and solubility difference of the set reference, respectively, and m is the number of extracts.

[0093] It should be added that ΔT and ΔS are extracted from the extraction and analysis technical specifications of anti-aging collagen extracts.

[0094] A7. Statistical evaluation of the physical property qualification index l1 of the target extract. z1, z2 and z3 are the weights of the set viscosity qualified index, appearance qualified index and solubility qualified index respectively, z1+z2+z3=1, z1>z2>z3.

[0095] The embodiments of the present invention extract the appearance information, solubility information and viscosity information of the anti-aging collagen extract, analyze the physical property qualification index of the anti-aging collagen extract, thereby breaking the current problem of insufficient attention to its solubility and viscosity, thereby ensuring the use effect of the anti-aging collagen extract, and at the same time improving the timeliness of the discovery of potential problems of the anti-aging collagen extract, thereby improving the safety of the anti-aging collagen extract.

[0096] It should be added that the viscosity of the anti-aging collagen extract will directly affect its use effect. For example, in cosmetics, appropriate viscosity can ensure that the product is easy to apply and spread, and evenly cover the skin surface, so as to better exert the anti-aging effect. Changes in appearance indirectly reflect the quality problems of the product. For example, darkening of color may mean that the extract has been oxidized, and precipitation may be due to unstable ingredients or contamination. Therefore, the weight of the appearance qualification index is less than that of the viscosity qualification index. At the same time, solubility may affect the ease of use and stability of the product, but its direct impact on the anti-aging effect of the product is small. Therefore, z1>z2>z3 is set. For the convenience of analysis, z1 can be specifically taken as 0.5, z2 can be specifically taken as 0.3, and z3 can be specifically taken as 0.2.

[0097] When analyzing the physical property qualification index of the anti-aging collagen extract, the embodiment of the present invention increases the importance of key properties by setting weights for the viscosity qualification index, the appearance qualification index and the solubility qualification index, thereby improving the pertinence of the physical property analysis. At the same time, by setting weights, the accuracy of the physical property analysis results is improved.

[0098] Step 4: Extraction of chemical properties of the extract: Extract the chemical property information of the target extract, which includes protein information and amino acid information.

[0099] It should be supplemented that the protein information of the target extract includes: the protein content of each extract, and the amino acid information of the target extract includes: the content of each amino acid in each extract.

[0100] It should be added that the specific process for obtaining the protein content of each extract is as follows: a certain amount of the extract is mixed with concentrated sulfuric acid and other reagents, heated for digestion, so that the protein in the extract is decomposed into ammonium salts, and the digested extract is distilled under alkaline conditions to convert the ammonium salts into ammonia gas and collected in a specific absorption liquid, and then the ammonia gas in the absorption liquid is titrated with a standard acid solution, and the nitrogen content in the extract is calculated based on the amount of acid consumed, and the protein content is calculated based on the nitrogen content and the protein conversion coefficient (generally 6.25).

[0101] It should also be added that the content of each amino acid in each extract is monitored by an amino acid analyzer.

[0102] Step 5: Chemical property analysis of the extract: Analyze the chemical property qualification index of the target extract based on the chemical property information of the target extract.

[0103] Exemplarily, the analysis of the chemical property qualification index of the target extract includes: extracting the protein content of each extract from the protein information of the target extract, and extracting the ideal protein content of the target extract from the parameter information of the target extract.

[0104] The protein content of each extract was compared with the ideal protein content. If the protein content of an extract was greater than or equal to the ideal protein content, the extract was recorded as an extract with qualified protein content, and the number of extracts with qualified protein content was counted.

[0105] The ratio of the number of extracts with qualified protein content to the number of extracts was taken as the qualified protein content index of the target polymer, denoted as

[0106] According to the amino acid information of the target extract, the amino acid qualification index of the target polymer is calculated

[0107] Furthermore, the statistical amino acid qualification index of the target polymer includes: extracting the content of each amino acid in each extract from the amino acid information of the target extract.

[0108] The ratio of the content of each amino acid to the total content of amino acids was taken as the amino acid composition ratio of each extract.

[0109] The amino acid composition ratio of each extract was compared with the corresponding amino acid composition ratio range of the reference.

[0110] If the amino acid composition ratios of an extract are all within the range of the corresponding reference amino acid composition ratios, the extract is recorded as an extract with qualified amino acid content, the number of extracts with qualified amino acid content is counted, and the ratio of the qualified amino acid content to the number of extracts is used as the qualified amino acid composition index of the target polymer, recorded as

[0111] Statistical target extract chemical property qualified index l2,

[0112] Step 6. Extract stability extraction: Extract the stability information of the target extract, which includes temperature stability information and light stability information.

[0113] It should be supplemented that the temperature stability information of the target extract includes: the appearance information, solubility information and viscosity information of each extract at each set temperature, and the light stability information of the target extract includes: the active ingredient content of each extract at each monitoring point under each set light intensity and the color data of the target extract.

[0114] It should also be added that the active ingredient content is monitored by high performance liquid chromatography.

[0115] Step 7. Extract stability analysis: Analyze the stability qualification index of the target extract based on the stability information of the target extract.

[0116] See also Figure 3 As shown, illustratively, the analysis of the stability qualification index of the target extract includes: B1, according to the temperature stability information of the target extract, calculating the temperature stability qualification index ψ1 of the target extract.

[0117] Furthermore, the statistical temperature stability qualified index of the target extract includes: B1-1, extracting the appearance information, solubility information and viscosity information of each extract at each set temperature from the temperature stability information of the target extract, and then analyzing in the same way as the analysis method of the physical property qualified index of the target extract to obtain the physical property qualified index of the target extract at each set temperature.

[0118] B1-2. With the set temperature as the horizontal axis and the physical property qualified index as the vertical axis, a physical property qualified index change curve of the target extract is constructed.

[0119] B1-3. Extract the reference change curve of the qualified index of the physical properties of the target extract from the parameter information of the target extract.

[0120] B1-4. Extract the length of the curve segment below the reference change curve of the physical property qualified index of the target extract, record it as L1, and record the length of the change curve of the physical property qualified index of the target extract as L2.

[0121] B1-5, statistical target extract temperature stability qualified index ψ1,

[0122] B2. According to the photostability information of the target extract, the photostability qualified index ψ2 of the target extract is calculated.

[0123] Furthermore, the statistical photostability qualified index of the target extract includes: B2-1, extracting the active ingredient content of each extract at each monitoring point under each set light intensity from the photostability information of the target extract, and then calculating the mean value to obtain the average active ingredient content of each extract, recorded as ξ i , and at the same time extract the maximum active ingredient content and the minimum active ingredient content of each extract.

[0124] B2-2. Difference between the maximum active ingredient content and the minimum active ingredient content of each extract is calculated to obtain the fluctuation range of the active ingredient content of each extract, which is recorded as ξ i ′.

[0125] B2-3. Statistical analysis of the qualified index σ of the active ingredient content of each extract i , ξ″ and ξ″′ are the active ingredient content and the fluctuation range of the active ingredient content of the set reference, respectively.

[0126] It should be added that ξ″ and ξ″′ are both extracted from the extraction and analysis technical specifications table of anti-aging collagen extracts.

[0127] B2-4, σ i The mean value is calculated and the calculated result is used as the qualified index of the change in the active ingredient content of the target extract, which is recorded as α1.

[0128] B2-5. Extract the color data of the target extract from the light stability information of the target extract, and then calculate the color change qualification index α2 of the target extract.

[0129] It should be added that the specific steps for calculating the color change qualified index of the target extract are as follows: extracting the brightness, red-green color and yellow-blue color of each monitoring point of each extract under a set light intensity from the color data of the target extract.

[0130] The initial lightness, initial red-greenness and initial yellow-blueness of the extract are denoted as w0, τ0 and b0 respectively. Meanwhile, the lightness, red-greenness and yellow-blueness of the extract at each monitoring point are denoted as w j, τ j and b j , j is the monitoring point number, j=1,2,.......n.

[0131] Statistical analysis of the total color difference ΔE of the extract at each monitoring point j ,

[0132] In a specific embodiment, if the color value of an extract at the initial stage is w0=80, τ0=-5, b0=10, and the color value at a certain monitoring point is w=78, τ=-4, b=11, the total color difference ΔE of the extract at the monitoring point is calculated,

[0133] If the total color difference of the extract at a certain monitoring point is less than the total color difference of the set reference, the monitoring point of the extract is recorded as a qualified monitoring point, the number of qualified monitoring points of the extract is counted, and the ratio of the qualified monitoring point to the number of monitoring points is used as the color qualified index of the extract, and then the color qualified index of each extract is obtained by statistics.

[0134] The color acceptance index of each extract is averaged and the calculated result is used as the color acceptance index of the target extract, which is recorded as α2.

[0135] B2-6. Filter out the minimum value from α1 and α2 as the photostability qualification index of the target extract and record it as ψ2.

[0136] B3. Statistical stability qualification index l3 of target extract, ψ1′ and ψ2′ are the temperature stability qualified index and light stability qualified index of the set reference respectively, and e is a natural constant.

[0137] It should be added that the ideal physical property qualified index of the target extract at each set temperature is extracted from the extraction and analysis technical specification table of the anti-aging collagen extract, and then the reference temperature stability qualified index ψ1′ is obtained by similar analysis according to the analysis method of ψ1, and the ideal active ingredient content and ideal color of each monitoring section under each set light intensity are extracted from the extraction and analysis technical specification table of the anti-aging collagen extract, and then the reference light stability qualified index ψ2′ is obtained by similar analysis according to the analysis method of ψ2.

[0138] Step 8. Extract quality qualification analysis: Analyze the quality qualification index of the target extract based on the physical property qualification index, chemical property qualification index and stability qualification index of the target extract.

[0139] Exemplarily, the quality qualified index of the analyzed target extract includes: a qualified index l1 of the physical properties of the target extract, a qualified index l2 of the chemical properties of the target extract, and a qualified index l3 of the stability of the target extract.

[0140] The quality qualified index θ of the target extract is calculated, θ=l1*q1+l2*q2+l3*q3, q1, q2 and q3 are the weights of the set physical property qualified index, chemical property qualified index and stability qualified index respectively, q1+q2+q3=1, q3>q2>q1.

[0141] It should be added that stability is directly related to whether the extract can maintain its anti-aging effect during storage and use. If the extract is unstable, it may degrade, deteriorate or lose its activity during storage, transportation or use, thus affecting the anti-aging effect and safety of the product. Chemical properties include protein content and amino acid composition, etc. These factors directly determine the characteristics of the anti-aging collagen extract, which in turn indirectly affects the efficacy and safety of the product. Physical properties include viscosity, appearance, solubility, etc. These factors mainly affect the product's usage experience and appearance, but have relatively little impact on the product's efficacy and safety. Therefore, q3>q2>q1 is set. For the convenience of analysis, q1 can be specifically taken as 0.2, q2 can be specifically taken as 0.3, and q3 can be specifically taken as 0.5.

[0142] Step 9. Feedback on extract quality: Provide corresponding feedback based on the quality qualification index of the target extract.

[0143] The embodiments of the present invention circumvent the current problem of insufficient attention to the physical properties and stability of anti-aging collagen extracts by conducting a comprehensive quality analysis of the physical properties, chemical properties and stability of anti-aging collagen extracts, while avoiding the limitations of single-dimensional analysis, thereby improving the comprehensiveness of the quality assessment of anti-aging collagen extracts.

[0144] The above contents are merely examples and explanations of the concept of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the concept of the invention or exceed the scope defined by the present invention, they shall all fall within the protection scope of the present invention.

Claims

1. An intelligent extraction and analysis method for anti-aging collagen extract, characterized in that: The method includes: Step 1: Extract information collection: randomly select a set number of anti-aging collagen extracts as target extracts, and collect parameter information of the target extracts; Step 2: Extraction of physical properties of the extract: Extraction of physical property information of the target extract, including appearance information, solubility information and viscosity information; Step 3: Analysis of physical properties of the extract: Analyze the physical property qualification index of the target extract according to the physical property information of the target extract; Step 4: Extraction of chemical properties of the extract: Extraction of chemical property information of the target extract, including protein information and amino acid information; Step 5: Chemical property analysis of the extract: Analyze the chemical property qualification index of the target extract according to the chemical property information of the target extract; Step 6: Extract stability extraction: extract the stability information of the target extract, the stability information includes temperature stability information and light stability information; Step 7: Extract stability analysis: Analyze the stability qualification index of the target extract according to the stability information of the target extract; Step 8. Extract quality qualification analysis: Analyze the quality qualification index of the target extract according to the physical property qualification index, chemical property qualification index and stability qualification index of the target extract; Step 9. Feedback on extract quality: Provide corresponding feedback based on the quality qualification index of the target extract.

2. The intelligent extraction and analysis method for anti-aging collagen extract according to claim 1, characterized in that: The physical property qualification index of the analytical target extract includes: A1. Extract the viscosity of each extract from the viscosity information of the target extract, denoted as η i , i is the extract number, i=1,2,......,m; A2, extracting the ideal viscosity range of the target polymer from the parameter information of the target extract, and recording the middle value of the ideal viscosity range of the target polymer as η′; A3. Calculate the viscosity qualification index β of each extract i , Δη is the viscosity difference of the set reference; A4. Calculate the average of the viscosity qualification index of each extract, and use the calculated result as the viscosity qualification index of the target extract, denoted as A5. According to the appearance information of the target extract, calculate the appearance qualification index of the target extract A6. According to the solubility information of the target extract, calculate the solubility qualification index of the target extract A7. Statistical evaluation of the physical property qualification index l1 of the target extract. z1, z2 and z3 are the weights of the set viscosity qualified index, appearance qualified index and solubility qualified index respectively, z1+z2+z3=1, z1>z2>z3.

3. The intelligent extraction and analysis method for anti-aging collagen extract according to claim 2, characterized in that: The statistical target extract appearance qualification index includes: extracting the color of each extract from the appearance information of the target extract, and extracting the ideal color range of the target extract from the parameter information of the target extract; Match the color of each extract with the ideal color range of the target extract. If the color of an extract is within the ideal color range, the extract is recorded as a color-qualified extract. The number of color-qualified extracts is counted and recorded as D1. Count the number of extracts, record it as D2, and take the ratio of D1 to D2 as the color qualification index of the target extract, record it as ε1; The diameter of each powder in each extract was extracted from the appearance information of the target extract, and then the average diameter and standard diameter difference of each extract were calculated and recorded as γ i and λ i ; The coefficient of variation CV of each extract was calculated i , The coefficient of variation of each extract is compared with the coefficient of variation of the set reference. If the coefficient of variation of an extract is less than or equal to the coefficient of variation of the set reference, the extract is recorded as a qualified extract with a diameter. The number of qualified extracts with diameters is counted, and the ratio of the qualified extract to the number of extracts is used as the qualified particle size index of the target extract, which is recorded as ε2. Statistical Appearance Qualification Index of Target Extract 4. The intelligent extraction and analysis method for anti-aging collagen extract according to claim 2, characterized in that: The solubility qualification index of the statistical target extract includes: The dissolution time and solubility of each extract after adding the set temperature and set volume solvent are extracted from the solubility information of the target extract and recorded as T i and S i ; The ideal dissolution time and ideal solubility of the target extract are extracted from the parameter information of the target extract, which are denoted as T′ and S′, respectively; Statistical solubility qualification index of target extract ΔT and ΔS are the dissolution time difference and solubility difference of the set reference, respectively, and m is the number of extracts.

5. The intelligent extraction and analysis method for anti-aging collagen extract according to claim 1, characterized in that: The chemical property qualification index of the analytical target extract includes: extracting the protein content of each extract from the protein information of the target extract, and extracting the ideal protein content of the target extract from the parameter information of the target extract; The protein content of each extract is compared with the ideal protein content. If the protein content of an extract is greater than or equal to the ideal protein content, the extract is recorded as an extract with qualified protein content, and the number of extracts with qualified protein content is counted; The ratio of the number of extracts with qualified protein content to the number of extracts was taken as the qualified protein content index of the target polymer, denoted as According to the amino acid information of the target extract, the amino acid qualification index of the target polymer is calculated Statistical target extract chemical property qualified index l2, 6. The intelligent extraction and analysis method for anti-aging collagen extract according to claim 5, characterized in that: The amino acid qualification index of the statistical target polymer includes: Extracting the content of each amino acid in each extract from the amino acid information of the target extract; The ratio of the content of each amino acid to the total content of amino acids was taken as the composition ratio of each amino acid in each extract; Compare the amino acid composition ratio of each extract with the corresponding amino acid composition ratio range of the reference; If the amino acid composition ratios of an extract are all within the range of the corresponding reference amino acid composition ratios, the extract is recorded as an extract with qualified amino acid content, the number of extracts with qualified amino acid content is counted, and the ratio of the qualified amino acid content to the number of extracts is used as the qualified amino acid composition index of the target polymer, recorded as 7. The intelligent extraction and analysis method for anti-aging collagen extract according to claim 1, characterized in that: The stability qualification index of the analytical target extract includes: B1. According to the temperature stability information of the target extract, calculate the temperature stability qualified index ψ1 of the target extract; B2. Calculate the photostability qualified index ψ2 of the target extract according to the photostability information of the target extract; B3. Statistical stability qualification index l3 of target extract, ψ1′ and ψ2′ are the temperature stability qualified index and light stability qualified index of the set reference respectively, and e is a natural constant.

8. The intelligent extraction and analysis method for anti-aging collagen extract according to claim 7, characterized in that: The temperature stability qualified index of the statistical target extract includes: Extracting the appearance information, solubility information and viscosity information of each extract at each set temperature from the temperature stability information of the target extract, and then analyzing the physical property qualified index of the target extract at each set temperature in the same way as the analysis method of the physical property qualified index of the target extract; With the set temperature as the horizontal coordinate and the physical property qualified index as the vertical coordinate, a physical property qualified index change curve of the target extract is constructed; Extracting a reference change curve of a qualified index of a physical property of a target extract from parameter information of the target extract; Extract the length of the curve segment below the reference change curve of the qualified index of the physical property of the target extract, record it as L1, and record the length of the change curve of the qualified index of the physical property of the target extract as L2; The temperature stability qualified index ψ1 of the target extract is statistically calculated.

9. The intelligent extraction and analysis method for anti-aging collagen extract according to claim 7, characterized in that: The statistical target extract's photostability qualification index includes: The active ingredient content of each extract at each monitoring point under each set light intensity was extracted from the photostability information of the target extract, and then the average active ingredient content of each extract was calculated, which was recorded as ξ i , and simultaneously extracting the maximum active ingredient content and the minimum active ingredient content of each extract therefrom; The maximum active ingredient content and the minimum active ingredient content of each extract were subtracted to obtain the fluctuation range of the active ingredient content of each extract, which was recorded as ξ i ′; Statistical analysis of the qualified index σ of the active ingredient content of each extract i , ξ″ and ξ″′ are the active ingredient content and the fluctuation range of the active ingredient content set as the reference, respectively; σ i The mean value is calculated, and the calculated result is used as the qualified index of the active ingredient content change of the target extract, which is recorded as α1; Extracting the color data of the target extract from the light stability information of the target extract, and then calculating the color change qualified index α2 of the target extract; The minimum value was selected from α1 and α2 as the photostability qualified index of the target extract and recorded as ψ2.

10. The intelligent extraction and analysis method for anti-aging collagen extract according to claim 9, characterized in that: The quality index of the analytical target extract includes: Extraction target extract physical property qualified index l1, target extract chemical property qualified index l2 and target extract stability qualified index l3; The quality qualified index θ of the target extract is calculated, θ=l1*q1+l2*q2+l3*q3, q1, q2 and q3 are the weights of the set physical property qualified index, chemical property qualified index and stability qualified index respectively, q1+q2+q3=1, q3>q2>q1.

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