Feed additive containing bacillus licheniformis and preparation method thereof

Abstract

The invention discloses a bacillus licheniformis-containing feed additive, and particularly relates to the field of feed additives, the bacillus licheniformis-containing feed additive comprises the following raw materials: sea-buckthorn, honeysuckle, sepiolite clay, chitosan, kelp powder, banana peel, compound trace elements, fish meal, a culture medium, amino acid, a mangosteen shell extract, bacillus licheniformis and the balance of corn flour. The nutritional broth culture medium is added, the taste of the feed can be improved by adding the nutritional broth culture medium, chitosan is added into the culture medium, when bacillus licheniformis is firstly mixed with the culture medium, the chitosan can enter the bacillus licheniformis, the activity of chitinase is stimulated, the activity of the bacillus licheniformis is better, and the taste of the feed is improved. And when the bacillus licheniformis is distributed in the feed additive, the bacillus licheniformis is coated with a culture medium containing chitosan, so that the growth environment of the bacillus licheniformis is better, and the growth promotion effect on animals is better.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, and more specifically to a feed additive containing Bacillus licheniformis and a preparation method thereof. Background Art

[0002] In the gastrointestinal tract, Bacillus maintains the ecological balance of the intestine mainly by biological oxygen deprivation. After short-term colonization in the intestine, it can consume a large amount of oxygen to maintain the anaerobic environment of the intestine and enhance the colonization of anaerobic bacteria. In addition, Bacillus can convert starch into monosaccharides in the intestine, and then other bacteria in the intestine convert these monosaccharides into lactic acid, which reduces the pH of the intestine and thus inhibits pathogens. Bacillus is significantly different from probiotic products made from other microorganisms. Bacillus can form spores under unfavorable conditions to protect itself and has a high resurrection rate. This type of product exists in the form of endospores and has many advantages: ① It is acid-resistant, salt-resistant, high-temperature-resistant (100°C) and extrusion-resistant, so it has high stability in the process of feed processing and pelleting, passing through the animal gastric acid environment, etc.; ② It has strong protease, lipase, and amylase activities, has a strong ability to degrade plant carbohydrates, can degrade certain complex compounds in plant feed, and produce polypeptide substances with the ability to antagonize intestinal pathogens. This type of bacteria has the ability to degrade many complex carbohydrates that are part of plants. The substances that can be degraded include triglycerides, proteins, starch, xylan, β-1,4-galactan, pectin, carboxymethyl cellulose, polygalacturonic acid, gum arabinan, glucose, p-nitrophenyl-pyranoglucoside, p-nitrophenyl-arabinofuranoside, lichenan and p-nitrophenyl-xylofuranose.

[0003] When used in feed, Bacillus licheniformis can induce the body to produce antibacterial active substances, inhibit the growth and reproduction of pathogenic bacteria, kill pathogens, so as to regulate the balance of intestinal flora, improve animal digestive function, enhance animal immunity, improve production performance and many other functions. It is suitable for intestinal flora imbalance caused by bacteria and poultry animals that need intestinal health care, such as chickens, ducks, geese, pigs and other animals.

[0004] However, the existing feed additives containing Bacillus licheniformis have poor activity and the growth-assisting effect on animals does not meet people's needs. Summary of the invention

[0005] The object of the present invention is to provide a feed additive containing Bacillus licheniformis and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0006] To achieve the above purpose, the present invention provides the following technical scheme: a feed additive containing Bacillus licheniformis, comprising the following raw materials in percentage by mass: 2-10% of seabuckthorn, 1-5% of honeysuckle, 2-8% of sepiolite clay, 2-6% of chitosan, 2-5% of kelp powder, 1-5% of banana peel, 0.5-1.5% of complex trace elements, 1-3% of fish meal, 2-5% of culture medium, 1-3% of amino acid, 1-5% of mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

[0007] In a preferred embodiment, the raw materials include the following mass percentages: 4-8% sea buckthorn, 2-4% honeysuckle, 4-6% sepiolite clay, 3-5% chitosan, 3-4% kelp powder, 2-4% banana peel, 0.8-1.2% complex trace elements, 1.5-2.5% fish meal, 3-4% culture medium, 1.5-2.5% amino acids, 2-4% mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

[0008] In a preferred embodiment, the raw materials include the following mass percentages: 6% sea buckthorn, 3% honeysuckle, 5% sepiolite clay, 4% chitosan, 3.5% kelp powder, 3% banana peel, 1% complex trace elements, 2% fish meal, 3.5% culture medium, 2% amino acid, 3% mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

[0009] In a preferred embodiment, the amount of Bacillus licheniformis added is 1×10 9 CFU / kg-5×10 9 CFU / kg, the complex trace elements are one or more combinations of copper, iron, zinc, magnesium, manganese, cobalt, selenium and potassium.

[0010] The present invention also provides a method for preparing a feed additive containing Bacillus licheniformis, and the specific preparation steps are as follows:

[0011] Step 1: Preparation of mangosteen shell extract, selecting fresh mangosteen shell, washing, drying and crushing, then adding 95% ethanol to extract three times under the assistance of ultrasound, combining the three filtrates, filtering and reducing pressure to recover ethanol to obtain a pre-extract, drying, crushing and screening the pre-extract to obtain a mangosteen shell extract for use;

[0012] Step 2: Wash and dry the weighed seabuckthorn, honeysuckle and banana peel, and grind them for the first time with a grinder to mix the coarse powder sample, sieve through a 30-50 mesh screen after the first grinding, and then grind the mixed coarse powder with a planetary ball mill to obtain ultrafine mixed dry powder for standby use;

[0013] Step 3: Weigh some chitosan and add it to the culture medium. Stir it evenly and sterilize it at 115-125°C for 15-20 minutes. Then inoculate Bacillus licheniformis and culture it at 32-36°C for later use.

[0014] Step 4: drying and crushing the weighed sepiolite clay, and then adding the remaining chitosan, kelp powder, complex trace elements, fish meal, amino acids and corn flour, stirring and mixing to obtain a premix;

[0015] Step 5: Add the mangosteen shell extract in step 1, the mixed dry powder in step 2 and the culture medium in step 3 to the premix in step 4, and mix well to obtain a feed additive containing Bacillus licheniformis.

[0016] In a preferred embodiment, in the step 1, 6-8 times of ethanol is added during the first extraction, and the extraction is carried out at 30-35°C for 30-40 min, 5-7 times of ethanol is added during the second extraction, and the extraction is carried out at 32-36°C for 30-35 min, and 4-6 times of ethanol is added during the third extraction, and the extraction is carried out at 35-38°C for 30-35 min. The ultrasonic auxiliary power in the step 1 is 200-300W.

[0017] In a preferred embodiment, the concentration of chitosan added in step 3 in the culture medium is 0.6%-1.2%, and the culture medium is a nutrient broth culture medium, which comprises the following ingredients: 100 ml beef broth, 10 g peptone and 5 g sodium chloride.

[0018] In a preferred embodiment, the preparation method of the culture medium is as follows: 50g of defatted and de-tendoned fresh beef mince is added with 100ml of water, stirred well and placed in a refrigerator overnight, taken out and boiled for 30 minutes, filtered with gauze, and then supplemented with water to 100ml, that is, beef broth, 10g of peptone and 5g of sodium chloride are added to the beef broth, heated to dissolve, the pH value is adjusted to 7.6, boiled for 10 minutes, filtered, and sterilized under high pressure to obtain the culture medium.

[0019] In a preferred embodiment, the drying temperature of the sepiolite clay in step 4 is 55-62° C., and the sepiolite clay is dried and crushed and then sieved through a 50-80 mesh sieve.

[0020] In a preferred embodiment, the mixed dry powder obtained after ball milling in step 2 is sieved through a 800-120 mesh sieve.

[0021] Technical effects and advantages of the present invention:

[0022] 1. A feed additive containing Bacillus licheniformis prepared by the raw material formula of the present invention, wherein a nutrient broth culture medium is added, and the culture medium in the feed additive can provide a better growth environment for Bacillus licheniformis, and the addition of the nutrient broth culture medium can improve the taste of the feed, and chitosan is added to the culture medium, and when Bacillus licheniformis is first mixed with the culture medium, the chitosan can enter the interior of the Bacillus licheniformis, stimulate the activity of chitinase, and make the activity of Bacillus licheniformis better, and when Bacillus licheniformis is distributed in the feed additive, the outside of the Bacillus licheniformis is coated with a culture medium containing chitosan, which can make the growth environment of Bacillus licheniformis better and have a better growth-promoting effect on animals, and by adding a mangosteen shell extract, the mangosteen shell extract contains more flavonoids and polyphenols, and can improve the antibacterial and antibacterial effects of animals;

[0023] 2. The present invention adds trace elements, seabuckthorn, honeysuckle, kelp powder, banana peel, fish meal, etc. The trace elements can increase the trace element content in the feed, making the nutrition of the animal more comprehensive, and the seabuckthorn fruit contains active substances such as multiple vitamins, fatty acids, trace elements, linoleic acid, seabuckthorn flavonoids, superoxide, and various amino acids required by the human body; the kelp powder is rich in iodine, minerals, vitamins, pigments, and active substances that can stimulate animal growth, and has a positive effect on the growth of animals, and the banana peel contains a large amount of dietary fiber such as pectin, oligosaccharides, cellulose, hemicellulose, lignin, etc. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Embodiment 1:

[0026] The invention provides a feed additive containing Bacillus licheniformis. The feed additive comprises the following raw materials in percentage by mass: 2% of seabuckthorn, 1% of honeysuckle, 2% of sepiolite clay, 2% of chitosan, 2% of kelp powder, 1% of banana peel, 0.5% of composite trace elements, 1% of fish meal, 2% of culture medium, 1% of amino acid, 1% of mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

[0027] In a preferred embodiment, the addition amount of Bacillus licheniformis is 3×10 9 CFU / kg, the complex trace elements are one or more combinations of copper, iron, zinc, magnesium, manganese, cobalt, selenium and potassium.

[0028] The present invention also provides a method for preparing a feed additive containing Bacillus licheniformis, and the specific preparation steps are as follows:

[0029] Step 1: Preparation of mangosteen shell extract, selecting fresh mangosteen shell, washing, drying and crushing, then adding 95% ethanol to extract three times under the assistance of ultrasound, combining the three filtrates, filtering and reducing pressure to recover ethanol to obtain a pre-extract, drying, crushing and screening the pre-extract to obtain a mangosteen shell extract for use;

[0030] Step 2: Wash and dry the weighed seabuckthorn, honeysuckle and banana peels, use a grinder to initially grind them to obtain a mixed coarse powder sample, sieve through a 40-mesh sieve after the initial grinding, and then use a planetary ball mill to grind the mixed coarse powder to obtain an ultrafine mixed dry powder for standby use;

[0031] Step 3: Weigh some chitosan and add it to the culture medium. Stir it evenly and sterilize it at 120°C for 18 minutes. Then inoculate Bacillus licheniformis and culture it at 35°C for later use.

[0032] Step 4: drying and crushing the weighed sepiolite clay, and then adding the remaining chitosan, kelp powder, complex trace elements, fish meal, amino acids and corn flour, stirring and mixing to obtain a premix;

[0033] Step 5: Add the mangosteen shell extract in step 1, the mixed dry powder in step 2 and the culture medium in step 3 to the premix in step 4, and mix well to obtain a feed additive containing Bacillus licheniformis.

[0034] In a preferred embodiment, in step one, 7 times of ethanol is added during the first extraction, and the extraction is carried out at 33°C for 35 minutes, 6 times of ethanol is added during the second extraction, and the extraction is carried out at 35°C for 33 minutes, and 5 times of ethanol is added during the third extraction, and the extraction is carried out at 37°C for 32 minutes. The ultrasonic auxiliary power in step one is 250W.

[0035] In a preferred embodiment, the concentration of chitosan added in step 3 in the culture medium is 1.0%, and the culture medium is a nutrient broth culture medium, and the culture medium comprises the following ingredients: 100 ml beef broth, 10 g peptone and 5 g sodium chloride.

[0036] In a preferred embodiment, the preparation method of the culture medium is as follows: 50g of defatted and de-tendoned fresh beef mince is added with 100ml of water, stirred well and placed in a refrigerator overnight, taken out and boiled for 30 minutes, filtered with gauze, and then supplemented with water to 100ml, that is, beef broth, 10g of peptone and 5g of sodium chloride are added to the beef broth, heated to dissolve, the pH value is adjusted to 7.6, boiled for 10 minutes, filtered, and sterilized under high pressure to obtain the culture medium.

[0037] In a preferred embodiment, the drying temperature of the sepiolite clay in step 4 is 58° C., and the sepiolite clay is dried, crushed, and then sieved through an 80-mesh sieve.

[0038] In a preferred embodiment, the mixed dry powder obtained after ball milling in step 2 is sieved through a 110-mesh sieve.

[0039] Embodiment 2:

[0040] Different from Example 1, a feed additive containing Bacillus licheniformis includes the following raw materials in percentage by mass: 6% sea buckthorn, 3% honeysuckle, 5% sepiolite clay, 4% chitosan, 3.5% kelp powder, 3% banana peel, 1% complex trace elements, 2% fish meal, 3.5% culture medium, 2% amino acid, 3% mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

[0041] Embodiment 3:

[0042] Different from Examples 1-2, a feed additive containing Bacillus licheniformis includes the following raw materials in percentage by mass: 10% sea buckthorn, 5% honeysuckle, 8% sepiolite clay, 6% chitosan, 5% kelp powder, 5% banana peel, 1.5% complex trace elements, 3% fish meal, 5% culture medium, 3% amino acid, 5% mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

[0043] Embodiment 4:

[0044] The invention provides a feed additive containing Bacillus licheniformis. The feed additive comprises the following raw materials in percentage by mass: 2% of seabuckthorn, 1% of honeysuckle, 2% of sepiolite clay, 2% of kelp powder, 1% of banana peel, 0.5% of composite trace elements, 1% of fish meal, 1% of amino acid, 1% of mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

[0045] In a preferred embodiment, the addition amount of Bacillus licheniformis is 3×10 9 CFU / kg, the complex trace elements are one or more combinations of copper, iron, zinc, magnesium, manganese, cobalt, selenium and potassium.

[0046] The present invention also provides a method for preparing a feed additive containing Bacillus licheniformis, and the specific preparation steps are as follows:

[0047] Step 1: Preparation of mangosteen shell extract, selecting fresh mangosteen shell, washing, drying and crushing, then adding 95% ethanol to extract three times under the assistance of ultrasound, combining the three filtrates, filtering and reducing pressure to recover ethanol to obtain a pre-extract, drying, crushing and screening the pre-extract to obtain a mangosteen shell extract for use;

[0048] Step 2: Wash and dry the weighed seabuckthorn, honeysuckle and banana peels, use a grinder to initially grind them to obtain a mixed coarse powder sample, sieve through a 40-mesh sieve after the initial grinding, and then use a planetary ball mill to grind the mixed coarse powder to obtain an ultrafine mixed dry powder for standby use;

[0049] Step 3: drying and crushing the weighed sepiolite clay, and then adding kelp powder, complex trace elements, fish meal, amino acids and corn flour, stirring and mixing to obtain a premix;

[0050] Step 4: Add the mangosteen shell extract in step 1, the mixed dry powder in step 2 and Bacillus licheniformis to the premix in step 4, and mix well to obtain a feed additive containing Bacillus licheniformis.

[0051] In a preferred embodiment, in step one, 7 times of ethanol is added during the first extraction, and the extraction is carried out at 33°C for 35 minutes, 6 times of ethanol is added during the second extraction, and the extraction is carried out at 35°C for 33 minutes, and 5 times of ethanol is added during the third extraction, and the extraction is carried out at 37°C for 32 minutes. The ultrasonic auxiliary power in step one is 250W.

[0052] In a preferred embodiment, the drying temperature of the sepiolite clay in step 3 is 58° C., and the sepiolite clay is dried, crushed, and then sieved through an 80-mesh sieve.

[0053] In a preferred embodiment, the mixed dry powder obtained after ball milling in step 2 is sieved through a 110-mesh sieve.

[0054] Embodiment 5:

[0055] The invention provides a feed additive containing Bacillus licheniformis. The feed additive comprises the following raw materials in percentage by mass: 2% of seabuckthorn, 1% of honeysuckle, 2% of sepiolite clay, 2% of chitosan, 0.5% of composite trace elements, 1% of fish meal, 2% of culture medium, 1% of amino acid, 1% of mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

[0056] In a preferred embodiment, the addition amount of Bacillus licheniformis is 3×10 9 CFU / kg, the complex trace elements are one or more combinations of copper, iron, zinc, magnesium, manganese, cobalt, selenium and potassium.

[0057] The present invention also provides a method for preparing a feed additive containing Bacillus licheniformis, and the specific preparation steps are as follows:

[0058] Step 1: Preparation of mangosteen shell extract, selecting fresh mangosteen shell, washing, drying and crushing, then adding 95% ethanol to extract three times under the assistance of ultrasound, combining the three filtrates, filtering and reducing pressure to recover ethanol to obtain a pre-extract, drying, crushing and screening the pre-extract to obtain a mangosteen shell extract for use;

[0059] Step 2: Wash and dry the weighed seabuckthorn and honeysuckle, use a grinder to initially grind them to obtain a mixed coarse powder sample, sieve through a 40-mesh sieve after the initial grinding, and then use a planetary ball mill to grind the mixed coarse powder to obtain an ultrafine mixed dry powder for standby use;

[0060] Step 3: Weigh some chitosan and add it to the culture medium. Stir it evenly and sterilize it at 120°C for 18 minutes. Then inoculate Bacillus licheniformis and culture it at 35°C for later use.

[0061] Step 4: drying and crushing the weighed sepiolite clay, and then adding the remaining chitosan, complex trace elements, fish meal, amino acids and corn flour, stirring and mixing to obtain a premix;

[0062] Step 5: Add the mangosteen shell extract in step 1, the mixed dry powder in step 2 and the culture medium in step 3 to the premix in step 4, and mix well to obtain a feed additive containing Bacillus licheniformis.

[0063] In a preferred embodiment, in step one, 7 times of ethanol is added during the first extraction, and the extraction is carried out at 33°C for 35 minutes, 6 times of ethanol is added during the second extraction, and the extraction is carried out at 35°C for 33 minutes, and 5 times of ethanol is added during the third extraction, and the extraction is carried out at 37°C for 32 minutes. The ultrasonic auxiliary power in step one is 250W.

[0064] In a preferred embodiment, the concentration of chitosan added in step 3 in the culture medium is 1.0%, and the culture medium is a nutrient broth culture medium, and the culture medium comprises the following ingredients: 100 ml beef broth, 10 g peptone and 5 g sodium chloride.

[0065] In a preferred embodiment, the preparation method of the culture medium is as follows: 50g of defatted and de-tendoned fresh beef mince is added with 100ml of water, stirred well and placed in a refrigerator overnight, taken out and boiled for 30 minutes, filtered with gauze, and then supplemented with water to 100ml, that is, beef broth, 10g of peptone and 5g of sodium chloride are added to the beef broth, heated to dissolve, the pH value is adjusted to 7.6, boiled for 10 minutes, filtered, and sterilized under high pressure to obtain the culture medium.

[0066] In a preferred embodiment, the drying temperature of the sepiolite clay in step 4 is 58° C., and the sepiolite clay is dried, crushed, and then sieved through an 80-mesh sieve.

[0067] In a preferred embodiment, the mixed dry powder obtained after ball milling in step 2 is sieved through a 110-mesh sieve.

[0068] Comparative Example:

[0069] The invention provides a feed additive, which comprises the following raw materials in percentage by mass: 1% of honeysuckle, 2% of sepiolite clay, 2% of chitosan, 0.5% of composite trace elements, 1% of fish meal, 1% of amino acid, and the balance of corn flour.

[0070] In a preferred embodiment, the addition amount of Bacillus licheniformis is 3×10 9 CFU / kg, the complex trace elements are one or more combinations of copper, iron, zinc, magnesium, manganese, cobalt, selenium and potassium.

[0071] The present invention also provides a method for preparing a feed additive containing Bacillus licheniformis, and the specific preparation steps are as follows:

[0072] Step 1: Wash and dry the weighed honeysuckle, use a grinder to initially grind it to mix the coarse powder sample, sieve it through a 40-mesh sieve after the initial grinding, and then use a planetary ball mill to ball-mill the mixed coarse powder to obtain ultrafine powder for standby use;

[0073] Step 2: drying and crushing the weighed sepiolite clay, then adding chitosan, complex trace elements, fish meal, amino acids and corn flour, stirring and mixing evenly to obtain a premix;

[0074] Step 3: adding the mixed dry powder to the premix in step 2, and mixing evenly to obtain a feed additive containing Bacillus licheniformis.

[0075] In a preferred embodiment, the drying temperature of the sepiolite clay in step 4 is 58° C., and the sepiolite clay is dried, crushed, and then sieved through an 80-mesh sieve.

[0076] In a preferred embodiment, the mixed dry powder obtained after ball milling in step 2 is sieved through a 110-mesh sieve.

[0077] The feed additives containing Bacillus licheniformis prepared in the above Examples 1-5 were respectively taken as experimental group 1, experimental group 2, experimental group 3, experimental group 4 and experimental group 5, and the feed additives produced in the comparative ratio were used as the control group for testing. The test animals were healthy, disease-free, and uniformly 30-day-old weaned piglets. Each piglet was weighed and recorded before morning feeding on the first and 30th days of the experiment. The average daily weight gain of the piglets was calculated, and after 12 hours of feeding suspension, 10 ml of blood was collected through the anterior vena cava using a blood collection tube containing sodium heparin, centrifuged, and the upper serum was separated and stored at -20°C. The ELISA kit was used to measure various immune indicators, and the test results are shown in Table 1:

[0078]

[0079] Table 1

[0080] As shown in Table 1, the feed additive containing Bacillus licheniformis produced by the present invention has a better effect on piglets, and the average daily weight gain and immune system are improved. Compared with the culture medium and chitosan in Example 1, the average daily weight gain and immune effect of Example 4 are reduced compared with Example 1, and compared with Example 1, the average daily weight gain and immune effect of Example 5 are reduced compared with Example 1. Therefore, by adding nutrient broth culture medium, the culture medium in the feed additive can provide a better growth environment for Bacillus licheniformis, and the addition of nutrient broth culture medium can To improve the taste of feed, chitosan is added to the culture medium. When Bacillus licheniformis is first mixed with the culture medium, chitosan can enter the interior of the Bacillus licheniformis and stimulate the activity of chitinase, making the activity of Bacillus licheniformis better. Moreover, when Bacillus licheniformis is distributed in the feed additive, the outside of the Bacillus licheniformis is coated with the culture medium containing chitosan, which can make the growth environment of Bacillus licheniformis better and promote the growth of animals better. Kelp powder is rich in iodine, minerals, vitamins, pigments and active substances that can stimulate animal growth, and has a positive effect on animal growth.

[0081] Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A feed additive containing Bacillus licheniformis, characterized in that: The invention comprises the following raw materials in percentage by mass: 2-10% of sea buckthorn, 1-5% of honeysuckle, 2-8% of sepiolite clay, 2-6% of chitosan, 2-5% of kelp powder, 1-5% of banana peel, 0.5-1.5% of complex trace elements, 1-3% of fish meal, 2-5% of culture medium, 1-3% of amino acid, 1-5% of mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

2. A feed additive containing Bacillus licheniformis according to claim 1, characterized in that: The invention comprises the following raw materials in percentage by mass: 4-8% of sea buckthorn, 2-4% of honeysuckle, 4-6% of sepiolite clay, 3-5% of chitosan, 3-4% of kelp powder, 2-4% of banana peel, 0.8-1.2% of complex trace elements, 1.5-2.5% of fish meal, 3-4% of culture medium, 1.5-2.5% of amino acid, 2-4% of mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

3. A feed additive containing Bacillus licheniformis according to claim 1, characterized in that: The invention comprises the following raw materials in percentage by weight: 6% of sea buckthorn, 3% of honeysuckle, 5% of sepiolite clay, 4% of chitosan, 3.5% of kelp powder, 3% of banana peel, 1% of complex trace elements, 2% of fish meal, 3.5% of culture medium, 2% of amino acid, 3% of mangosteen shell extract and Bacillus licheniformis, and the balance is corn flour.

4. A feed additive containing Bacillus licheniformis according to claim 1, characterized in that: The addition amount of Bacillus licheniformis is 1×10 9 CFU / kg-5×10 9 CFU / kg, the complex trace elements are one or more combinations of copper, iron, zinc, magnesium, manganese, cobalt, selenium and potassium.

5. A method for preparing a feed additive containing Bacillus licheniformis, characterized in that: The specific preparation steps are as follows: Step 1: Preparation of mangosteen shell extract, selecting fresh mangosteen shell, washing, drying and crushing, then adding 95% ethanol to extract three times under the assistance of ultrasound, combining the three filtrates, filtering and reducing pressure to recover ethanol to obtain a pre-extract, drying, crushing and screening the pre-extract to obtain a mangosteen shell extract for use; Step 2: Wash and dry the weighed seabuckthorn, honeysuckle and banana peel, and grind them for the first time with a grinder to mix the coarse powder sample, sieve through a 30-50 mesh screen after the first grinding, and then grind the mixed coarse powder with a planetary ball mill to obtain ultrafine mixed dry powder for standby use; Step 3: Weigh some chitosan and add it to the culture medium. Stir it evenly and sterilize it at 115-125°C for 15-20 minutes. Then inoculate Bacillus licheniformis and culture it at 32-36°C for later use. Step 4: drying and crushing the weighed sepiolite clay, and then adding the remaining chitosan, kelp powder, complex trace elements, fish meal, amino acids and corn flour, stirring and mixing to obtain a premix; Step 5: Add the mangosteen shell extract in step 1, the mixed dry powder in step 2 and the culture medium in step 3 to the premix in step 4, and mix well to obtain a feed additive containing Bacillus licheniformis.

6. The method for preparing a feed additive containing Bacillus licheniformis according to claim 5, characterized in that: In the step 1, 6-8 times of ethanol is added during the first extraction, and the extraction is carried out at 30-35°C for 30-40 minutes. In the second extraction, 5-7 times of ethanol is added, and the extraction is carried out at 32-36°C for 30-35 minutes. In the third extraction, 4-6 times of ethanol is added, and the extraction is carried out at 35-38°C for 30-35 minutes. The ultrasonic auxiliary power in the step 1 is 200-300W.

7. The method for preparing a feed additive containing Bacillus licheniformis according to claim 5, characterized in that: The concentration of chitosan added in the step 3 in the culture medium is 0.6%-1.2%. The culture medium is a nutrient broth culture medium, and the culture medium includes the following ingredients: 100 ml beef broth, 10 g peptone and 5 g sodium chloride.

8. The method for preparing a feed additive containing Bacillus licheniformis according to claim 7, characterized in that: The preparation method of the culture medium is as follows: 50g of defatted and de-tendoned fresh beef mince is added with 100ml of water, stirred evenly and placed in a refrigerator overnight, taken out and boiled for 30 minutes, filtered with gauze, and then supplemented with water to 100ml to obtain beef broth, 10g of peptone and 5g of sodium chloride are added to the beef broth, heated to dissolve, the pH value is adjusted to 7.6, boiled for 10 minutes, filtered, and sterilized under high pressure to obtain the culture medium.

9. The method for preparing a feed additive containing Bacillus licheniformis according to claim 5, characterized in that: The drying temperature of the sepiolite clay in step 4 is 55-62° C., and the sepiolite clay is dried, crushed, and then sieved through a 50-80 mesh sieve.

10. The method for preparing a feed additive containing Bacillus licheniformis according to claim 5, characterized in that: The mixed dry powder obtained after ball milling in the step 2 is sieved through a 800-120 mesh sieve.

Images