A method and device for cultivating integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge

Abstract

The present invention discloses a method and device for culturing integrated shortcut nitrification anaerobic ammonium oxidation granular sludge. The method includes: a biological selector, an aeration tank, and a sedimentation selector connected in series by overflow; inoculating aerobic granular sludge and integrated shortcut nitrification anaerobic ammonium oxidation sludge in the biological selector and the aeration tank, wherein the proportion of the integrated shortcut nitrification anaerobic ammonium oxidation sludge is 5%-10%. This method utilizes the fact that aerobic granular sludge and shortcut nitrification anaerobic ammonium oxidation granular sludge have similar microbial stratification structures, and promotes the rapid formation of integrated shortcut nitrification anaerobic ammonium oxidation granular sludge based on the transformation of microbial ecological niches through the ecological niche replacement of anaerobic ammonium oxidizing bacteria. Part of the effluent from the sedimentation selector is refluxed to increase the upward flow velocity of the liquid in the sedimentation selector to promote the elutriation of flocculent sludge and the enrichment of granular sludge. This method is convenient to operate and simple to control, can be applied to continuous flow processes, and helps the rapid startup and stable operation of the integrated shortcut anaerobic ammonium oxidation system.

Description

Technical Field

[0001] The present invention belongs to the technical field of integrated short-cut nitrification anaerobic ammonium oxidation granular sludge cultivation, and more specifically, relates to a method and device for cultivating integrated short-cut nitrification anaerobic ammonium oxidation granular sludge. Background Art

[0002] The integrated, short-cut anaerobic nitrification / anaerobic ammonium oxidation (PN / A) process combines anaerobic ammonium-oxidizing bacteria (AnAOB) and ammonia-oxidizing bacteria (AOB) in a single reactor. The AOB oxidizes a portion of the incoming ammonia nitrogen into nitrite nitrogen. Subsequently, the AnAOB directly converts the remaining ammonia nitrogen and nitrite nitrogen into nitrogen gas. The PN / A process is a promising alternative to conventional nitrification / denitrification methods because it requires no organic matter and saves aeration energy.

[0003] Despite the increasing importance of PN / A globally, its large-scale utilization remains difficult, with the enrichment of AnAOB remaining a major bottleneck due to its low growth rate (0.065-0.33 days). -1 ) and are significantly sensitive to various environments. People have adopted a variety of strategies to enrich slow-growing AnAOB and enhance biomass cultivation, among which sludge granulation is a feasible and economical method. In recent years, a large number of researchers have carried out research on the granulation of anaerobic ammonium oxidation sludge. For example, Lin Xiangyu et al. invented "A method for rapid in-situ expansion of granular sludge", which uses the crystallization / precipitation formed by metal cations and phosphates, and the different charges of metal cations and activated sludge as the crystal nuclei and internal driving force for the aggregation of granular sludge to form granular sludge; Zhang Jing et al. invented "A method for rapid formation of anaerobic ammonium oxidation granular sludge", which promotes the granulation of anaerobic ammonium oxidation sludge by adding 30C8-HSL quorum sensing signal molecules; Gong Hui et al. invented "A method for promoting rapid granulation of anaerobic ammonium oxidation bacteria using coagulants", which achieves rapid granulation by adding coagulants to control the protein concentration and Zata potential value of extracellular polymers of microbial aggregates at different stages of granulation. In addition, inert carriers such as activated carbon, zeolite, and bentonite can also be added, but the effect is greatly affected by the density of the carrier. Although the above methods promote the granulation of anaerobic ammonium oxidation sludge, they require the addition of different types of substances, which may cause secondary pollution, and the operation is complicated and costly. Summary of the Invention

[0004] The purpose of the present invention is to address the deficiencies in the prior art and provide a method and device for cultivating integrated short-range nitrification anaerobic ammonium oxidation granular sludge, so as to solve the problems that the existing anaerobic ammonium oxidation sludge granular cultivation requires additional addition of different types of substances, which may cause secondary pollution, and is complicated to operate and has high costs.

[0005] To achieve the above object, the present invention provides a method for culturing integrated shortcut nitrification and anaerobic ammonium oxidation granular sludge, comprising:

[0006] A biological selection tank, an aeration tank, and a sedimentation selection tank connected in overflow sequence;

[0007] S1. Inoculate aerobic granular sludge and integrated shortcut nitrification and anaerobic ammonium oxidation sludge with a total concentration of 5000-6000 mg / L in the biological selection tank and the aeration tank, wherein the proportion of integrated shortcut nitrification and anaerobic ammonium oxidation sludge is 5%-10%;

[0008] S2. Pump in wastewater with high ammonia nitrogen and low C / N, control the ammonia nitrogen concentration in the biological selection tank at 200-400 mg / L, and the ammonia nitrogen concentration in the aeration tank at 20-100 mg / L. Utilize the difference in pollutant concentration between the biological selection tank and the aeration tank to promote the secretion of microbial extracellular polymers, maintain the morphology of aerobic granular sludge, and efficiently retain anaerobic ammonium oxidation bacteria and rapidly enrich and grow them;

[0009] S3. Control the influent nitrogen load at 0.2 kgN / m 3 / d, the temperatures of the biological selection tank and the aeration tank at 30-35 °C, the pH at 7.5-8.0, and return the reflux sludge from the sedimentation selection tank to the biological selection tank;

[0010] S4. When the total nitrogen removal rate is not less than 80% for three consecutive days, gradually increase the influent volume until the influent nitrogen load reaches 1.0 kgN / m 3 / d. Partially return the effluent from the sedimentation selection tank to the sedimentation selection tank for washing of flocculent sludge to enrich granular sludge.

[0011] Optionally, aerobic bacteria are distributed on the outer layer of the aerobic granular sludge, and anaerobic bacteria are distributed on the inner layer.

[0012] Optionally, the particle size of the aerobic granular sludge is 100-500 μm.

[0013] Optionally, part of the effluent from the sedimentation selection tank is refluxed to achieve a liquid rising velocity of 1.0-10.0 m / h in the sedimentation selection tank, promote the washing of light flocculent sludge, and enrich granular sludge.

[0014] Optionally, it further includes: using a particle interception device to intercept the particles lost in the effluent and return them to the biological selection tank to reduce the loss of granular sludge.

[0015] The present invention also provides a device for culturing integrated shortcut nitrification and anaerobic ammonium oxidation granular sludge, comprising:

[0016] A biological selection tank, an aeration tank, and a sedimentation selection tank connected in overflow sequence;

[0017] The sludge outlet end of the sedimentation and selection tank is connected to the biological selection tank through a sludge reflux pump;

[0018] A particle interception device and a circulation pump are arranged at the water outlet end of the sedimentation and selection tank. Part of the effluent is refluxed to the water inlet end of the sedimentation and selection tank through the circulation pump, and the lost granular sludge is intercepted by the particle interception device.

[0019] Optionally, a raw water tank is arranged at the water inlet end of the biological selection tank, and aerobic granular sludge is directly added to the biological selection tank and the aeration tank.

[0020] Optionally, an aerator is arranged in the aeration tank, and the aerator is connected to a blower through a gas flow meter.

[0021] Optionally, the sedimentation and selection tank includes:

[0022] A pool body, a sludge hopper is arranged at the lower end of the pool body, and an overflow weir is arranged at the upper end of the pool body;

[0023] A central cylinder, the central cylinder is arranged in the middle of the pool body, a diffuser with a gradually expanding structure is arranged at the lower end, and the upper end is higher than the overflow weir and is connected to the water outlet end of the aeration tank.

[0024] Optionally, the particle interception device is connected to the biological selection tank.

[0025] The present invention provides a method for culturing integrated shortcut nitrification anaerobic ammonium oxidation granular sludge, and its beneficial effects are as follows:

[0026] This method is applicable to continuous flow processes. Utilizing the fact that aerobic granular sludge and shortcut nitrification anaerobic ammonium oxidation granular sludge have similar microbial stratified structures, with aerobic bacteria distributed in the outer layer and anaerobic bacteria distributed in the inner layer, and similar microbial ecological niches. Through the ecological niche replacement of anaerobic ammonium oxidizing bacteria, it can promote the granulation of integrated shortcut nitrification anaerobic ammonium oxidation granular sludge based on the transformation of microbial ecological niches, achieve the rapid cultivation of integrated shortcut nitrification anaerobic ammonium oxidation granular sludge, and at the same time, no additional materials need to be added and there is no secondary pollution.

[0027] Other features and advantages of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] By describing the exemplary embodiments of the present invention in more detail in conjunction with the drawings, the above and other objects, features, and advantages of the present invention will become more obvious. Among them, in the exemplary embodiments of the present invention, the same reference numerals generally represent the same components.

[0029] Figure 1Shows a schematic structural diagram of a device for culturing integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention.

[0030] Figure 2 Shows a schematic diagram of aerobic granular sludge in a method for culturing integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention.

[0031] Figure 3 Shows a schematic diagram of integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge cultured by a method for culturing integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention.

[0032] Figure 4 Shows a graph of the change in nitrogen removal load during the startup stage of a method for culturing integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention.

[0033] Figure 5 Shows a graph of the change in particle size of integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge in a method for culturing integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention.

[0034] Description of reference numerals:

[0035] 1.1, raw water tank; 2.1, biological selection tank; 2.2, aeration tank; 2.3, sludge reflux pump; 2.4, gas flowmeter; 2.5, blower; 2.6, aerator; 3.1, sedimentation selection tank; 3.2, circulation pump; 3.3, particle retention device. Detailed implementation manners

[0036] The preferred embodiments of the present invention will be described in more detail below. Although the preferred embodiments of the present invention are described below, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0037] As Figures 1-5 shown, a device for culturing integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge includes:

[0038] A biological selection tank 2.1, an aeration tank 2.2, and a sedimentation selection tank 3.1 that are connected in series and overflow;

[0039] S1. Inoculate aerobic granular sludge and integrated short-cut nitrification and anaerobic ammonium oxidation sludge with a total concentration of 5000 - 6000 mg / L in the biological selection tank 2.1 and the aeration tank 2.2, where the proportion of integrated short-cut nitrification and anaerobic ammonium oxidation sludge is 5% - 10%;

[0040] S2. Pump in wastewater with high ammonia nitrogen and low C / N, control the ammonia nitrogen concentration in the biological selection tank 3.1 at 200 - 400 mg / L, the ammonia nitrogen concentration in the aeration tank 2.2 at 20 - 100 mg / L, and the DO at 0.3 - 1.0 mg / L. Utilize the difference in pollutant concentrations between the biological selection tank and the aeration tank to promote the secretion of microbial extracellular polymers, maintain the morphology of aerobic granular sludge, and efficiently retain anaerobic ammonium-oxidizing bacteria and rapidly enrich and grow them.

[0041] S3. Control the influent nitrogen load at 0.2 kgN / m 3 / d, keep the temperature in the biological selection tank 2.1 and the aeration tank 2.2 at 30 - 35 °C, the pH at 7.5 - 8.0, and return the reflux sludge from the sedimentation selection tank 3.1 to the biological selection tank 2.1.

[0042] S4. When the total nitrogen removal rate is not less than 80% for at least three consecutive days, gradually increase the influent flow rate until the influent nitrogen load reaches 1.0 kgN / m 3 / d. Partially return the effluent from the sedimentation selection tank 3.1 to the sedimentation selection tank 3.1 for washing of flocculent sludge to enrich granular sludge.

[0043] Specifically, the aerobic granular sludge used in this method is simple to obtain, has a low start-up cost for granulation, does not require the addition of other substances, has no secondary pollution, and has a simple operation process.

[0044] Among them, the aerobic granular sludge has good sedimentation performance and a large biomass, and is an excellent biological carrier. In the case of insufficient integrated shortcut nitrification and anaerobic ammonium oxidation sludge, introducing aerobic granular sludge can strengthen the retention of anaerobic ammonium-oxidizing bacteria, rapidly enrich anaerobic ammonium-oxidizing bacteria to achieve the start-up of the reactor, and achieve the rapid cultivation of a large amount of integrated shortcut nitrification and anaerobic ammonium oxidation sludge by using a small amount of integrated shortcut nitrification and anaerobic ammonium oxidation sludge as seeds.

[0045] The pollutant concentrations in the biological selection tank and the aeration tank are different, forming a concentration difference environment. The continuous flow enables the granular sludge to cycle alternately in the feast / starvation environment, promoting the secretion of microbial extracellular polymers and maintaining the granular morphology of aerobic granular sludge.

[0046] The sedimentation selection tank adjusts the upward flow velocity in the sedimentation selection tank by adjusting the reflux flow rate to achieve the effect of washing light flocs and rapidly enriching and growing integrated shortcut nitrification and anaerobic ammonium oxidation granular sludge.

[0047] In this embodiment, aerobic bacteria are distributed on the outer layer of the aerobic granular sludge, and anaerobic bacteria are distributed on the inner layer.

[0048] Specifically, the inoculated sludge is mainly aerobic granular sludge. Taking advantage of the good settling property and large biomass of aerobic granular sludge, as a biological carrier, the internal anaerobic bacteria are replaced with AnAOB to achieve the efficient interception and enrichment of AnAOB. Generally, it takes at least 3 - 6 months for AnAOB to form granular sludge by secreting extracellular proteins on its own. In this solution, by introducing aerobic granular sludge, the transformation of aerobic granular sludge into visible red granules can be achieved in 1 month.

[0049] In this embodiment, the particle size of the aerobic granular sludge is 100 - 500 μm.

[0050] Specifically, selecting aerobic granular sludge with an appropriate particle size is conducive to the ecological position replacement of AnAOB, avoiding the inability to form an effective anaerobic layer after replacement due to too small a particle size, which affects the activity of AnAOB, or the increase in thickness due to too large a particle size, making internal replacement difficult.

[0051] In this embodiment, a part of the effluent from the circulating sedimentation selection tank 3.1 is refluxed to achieve an upward flow rate of 1.0 - 10.0 m / h in the sedimentation selection tank 3.1, promoting the elutriation of light flocculent sludge and enriching granular sludge.

[0052] In this embodiment, it further includes: using the particle interception device 3.3 to intercept the effluent - lost particles and return them to the biological selection tank 2.1 to reduce the loss of granular sludge.

[0053] The present invention also provides a device for culturing integrated short - cut nitrification anaerobic ammonium oxidation granular sludge, including:

[0054] A biological selection tank 2.1, an aeration tank 2.2, and a sedimentation selection tank 3.1 that are connected in series with overflow;

[0055] The sludge outlet end of the sedimentation selection tank 3.1 is connected to the biological selection tank 2.1 through a sludge reflux pump 2.3;

[0056] The effluent end of the sedimentation selection tank 3.1 is provided with a particle interception device 3.3 and a circulation pump 3.2. A part of the effluent is refluxed to the inlet end of the sedimentation selection tank 3.1 through the circulation pump 3.2, and the lost granular sludge is intercepted by the particle interception device 3.3.

[0057] Specifically, aerobic granular sludge and shortcut nitrification and anaerobic ammonium oxidation (Anammox) granular sludge have similar microbial stratification structures. Aerobic bacteria are distributed in the outer layer and anaerobic bacteria are distributed in the inner layer. Their microbial niches are similar. Through the niche replacement of AnAOB, the granulation of integrated shortcut nitrification and Anammox granular sludge based on the transformation of microbial niches can be promoted, and the preferred particle size is 100-500 μm. During use, aerobic granular sludge and a small amount of integrated shortcut nitrification and Anammox sludge are inoculated in the biological selector 2.1. By utilizing the similar microbial niches of aerobic granular sludge and shortcut nitrification and Anammox granular sludge, as well as the characteristics of good sedimentation performance and large biomass of aerobic granular sludge, it can be used as a biological carrier to rapidly enrich AnAOB to form integrated shortcut nitrification and Anammox granular sludge. The rising flow velocity of the water flow in the sedimentation selector 3.2 is increased by circulating the effluent to realize the elutriation of light flocculent sludge and promote the enrichment of granular sludge. The granular sludge intercepted from the effluent of the sedimentation selector 3.1 is returned to the biological selector 2.1 for further cultivation of granular sludge.

[0058] Furthermore, the volume ratio of the biological selector 2.1 to the aeration tank 2.2 is 0.1-0.6; the water is evenly distributed at the bottom of the biological selector 2.1, and the height-diameter ratio is 4-6. When the actual height-diameter ratio requirement cannot be met, multiple groups of parallel biological selectors 2.1 can be set; the sludge reflux pump 2.3 adopts a positive displacement pump such as a screw centrifugal pump or a diaphragm pump with little damage to the particles.

[0059] In this embodiment, a raw water tank 1.1 is arranged at the water inlet end of the biological selector 2.1, and aerobic granular sludge is directly added to the biological selector 2.1 and the aeration tank 2.2.

[0060] Specifically, the water quality of the influent is pre-adjusted through the raw water tank 1.1.

[0061] In this embodiment, an aerator 2.6 is arranged in the aeration tank 2.2, and the aerator 2.6 is connected to a blower 2.5 through a gas flow meter 2.4.

[0062] Specifically, the air flow rate is accurately controlled through the gas flow meter 2.4.

[0063] In this embodiment, the sedimentation selector 3.1 includes:

[0064] A pool body, a sludge hopper is arranged at the lower end of the pool body, and an overflow weir is arranged at the upper end of the pool body;

[0065] A central cylinder, the central cylinder is arranged in the middle of the pool body, a diffuser with a gradually expanding structure is arranged at the lower end, and the upper end is higher than the overflow weir and is connected to the water outlet end of the aeration tank 2.2.

[0066] Specifically, the bottom of the central cylinder of the sedimentation selector 3.1 is 0.3-0.5 m away from the bottom of the pool to impact the sludge deposited at the bottom and enhance the sludge selection effect.

[0067] In this embodiment, the particle interception device 3.3 is connected to the biological selection tank 2.1.

[0068] Specifically, the granular sludge is circulated through the particle interception device to prevent the loss of granular sludge.

[0069] Embodiment

[0070] The device for rapidly culturing integrated shortcut nitrification anaerobic ammonium oxidation granular sludge includes: a raw water tank 1.1, a biological selection tank 2.1, an aeration tank 2.2, and a sedimentation selection tank 3.1; the raw water tank 1.1 is connected to the biological selection tank 2.1 through a feed water pump 1.2, and the muddy water mixture flowing out from the upper part of the biological selection tank 2.1 enters the aeration tank 2.2. The aeration tank 2.2 includes a blower 2.5, an aerator 2.6, and a gas flow meter 2.4. Aeration is carried out in the aeration tank 2.2 through the blower 2.5. The aerator 2.6 is installed at the bottom of the tank and is connected to the blower 2.5 through an air pipeline, and the gas flow rate is adjusted by the gas flow meter 2.4; the water discharged from the aeration tank 2.2 enters the central cylinder of the sedimentation selection tank 3.1. The sedimentation selection tank 3.1 is connected to the biological selection tank 2.1 through a sludge return pump 2.3. A part of the discharged water is connected to the outlet pipe of the aeration tank 2.2 through an outlet water circulation pump 3.2 and enters the central cylinder of the sedimentation selection tank 3.1 to increase the rising flow rate of the sedimentation selection tank; the remaining part of the discharged water enters the particle interception device 3.2, the intercepted particles are returned to the aeration tank 2.2, and the discharged water is discharged.

[0071] The biological selection tank 2.1 is evenly distributed with water at the bottom, and the height-diameter ratio is 5. There is an annular outlet weir at the upper part of the biological selection tank 2.1, and the muddy water mixture in the tank enters the aeration tank 2.2 through the outlet weir. The volume ratio of the biological selection tank 2.1 to the aeration tank 2.2 is 0.3. The bottom of the central cylinder of the sedimentation selection tank 3.1 is 0.3 m away from the bottom of the tank to impact the sedimented sludge at the bottom and enhance the sludge selection effect; the sludge return pump 2.3 adopts a screw centrifugal pump with little damage to the particles. Online instruments and automatic control components can be configured to realize the automatic control of the system.

[0072] The operation method of the device for rapidly culturing integrated shortcut nitrification anaerobic ammonium oxidation granular sludge of the present invention includes the following steps:

[0073] (1) Inoculating sludge: 90% aerobic granular sludge and 10% integrated anaerobic ammonium oxidation sludge are inoculated in the biological selection tank 2.1 and the aeration tank 2.2, and the total concentration reaches 6000 mg / L. The particle size of the aerobic granular sludge is 300 μm.

[0074] (2) Startup stage: The raw water tank 1.1 contains high-ammonia-nitrogen and low C / N wastewater configured by adding ammonium bicarbonate and sodium bicarbonate to the effluent of the primary sedimentation tank of urban sewage. The ammonia-nitrogen concentration is 300 - 600 mg / L, and the C / N is less than 1.5. The wastewater is continuously pumped into the biological selection tank 2.1 by the inlet pump 1.2, and the ammonia-nitrogen concentration in the biological selection tank is controlled at 200 - 400 mg / L; the effluent of the biological selection tank 2.1 enters the aeration tank 2.2, and the ammonia-nitrogen concentration in the aeration tank 2.2 is controlled at 20 - 100 mg / L. By using the difference in pollutant concentrations between the biological selection tank 2.1 and the aeration tank 2.2, the secretion of microbial extracellular polymers is promoted, and the granular morphology of aerobic granular sludge is maintained. The blower 2.5 of the aeration tank 2.2 is turned on to supply air to the aeration tank 2.2 through the aerator 2.6, and the air volume of the aeration tank is controlled by the gas flow meter 2.4 to keep the DO at 0.3 - 1.0 mg / L; the temperatures of the biological selection tank 2.1 and the aeration tank 2.2 are controlled at 30 - 35 °C, the pH is at 7.5 - 8.0, the influent nitrogen load is at 0.2 kgN / m 3 / d, the sludge return pump 2.3 is turned on, and the sludge return ratio is controlled at 0.3 - 1.0. The effluent of the sedimentation selection tank 3.1 enters the particle interception device 3.3; the effluent water quality is measured. When the total nitrogen removal rate reaches 80% for three consecutive days, the reactor startup is completed.

[0075] (3) Rapid cultivation of integrated anaerobic ammonium oxidation granular sludge: Aerobic granular sludge and shortcut nitrification anaerobic ammonium oxidation granular sludge have similar microbial stratification structures, with aerobic bacteria distributed in the outer layer and anaerobic bacteria distributed in the inner layer. Their microbial ecological niches are similar, and the cultivation of integrated anaerobic ammonium oxidation granular sludge can be accelerated through the ecological niche replacement of AnAOB. By using the different pollutant concentrations in the biological selection tank 2.1 and the aeration tank 2.2, a feast / famine environment is maintained to promote the transformation of microbial ecological niches and form integrated anaerobic ammonium oxidation granular sludge. The difference in pollutant concentrations between the biological selection tank 2.1 and the aeration tank 2.2 is maintained, and the influent flow rate is adjusted to gradually increase the influent nitrogen load to 1.0 kgN / m 3 / d. At the same time, the effluent circulation pump 3.2 of the sedimentation selection tank 3.1 is turned on. According to the growth of the sludge concentration in the aeration tank 2.2, the flow rate of the effluent circulation pump 3.2 and the upward flow rate of the sedimentation selection tank 3.1 are gradually increased for the elutriation of flocculent sludge and the enrichment cultivation of granular sludge. The adjustment range of the upward flow rate is 1.0 - 10.0 m / h, and the effluent enters the particle interception device 3.3 to achieve the rapid enrichment and growth of granular sludge.

[0076] Under the above conditions, it was found after 30 days of reactor operation that the total nitrogen removal load increased from 0.2 kgN / m 3 / d to 0.6 kgN / m 3 / d, the color of the aerobic granular sludge changed to an obvious red, the particle size increased from 250 μm to 310 μm, and visible red particles were formed.

[0077] The embodiments of the present invention have been described above. The above description is exemplary and not exhaustive, and is also not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments.

Claims

1. A method for culturing integrated short-cut nitrification anaerobic ammonium oxidation granular sludge, characterized in that, Comprising: A biological selection tank, an aeration tank, and a sedimentation selection tank that are connected in series by overflow; S1. Inoculate aerobic granular sludge and integrated shortcut nitrification anaerobic ammonium oxidation sludge with a total concentration of 5000 - 6000 mg / L in the biological selection tank and the aeration tank, where the proportion of the integrated shortcut nitrification anaerobic ammonium oxidation sludge is 5% - 10%; S2. Pump in wastewater with high ammonia nitrogen and low C / N, control the ammonia nitrogen concentration in the biological selection tank at 200 - 400 mg / L, and the ammonia nitrogen concentration in the aeration tank at 20 - 100 mg / L. Utilize the difference in pollutant concentrations between the biological selection tank and the aeration tank to promote the secretion of microbial extracellular polymers, maintain the morphology of aerobic granular sludge, and efficiently retain anaerobic ammonium oxidizing bacteria and rapidly enrich and grow them; S3. Control the influent nitrogen load at 0.2 kgN / m 3 / d, keep the temperatures of the biological selector and the aeration tank at 30 - 35 °C, the pH at 7.5 - 8.0, and return the sludge from the sedimentation selector to the biological selector; S4. The total nitrogen removal rate is not less than 80% for three consecutive days, and the influent flow rate is gradually increased until the influent nitrogen load reaches 1.0 kgN / m 3 / d. A part of the effluent from the sedimentation and selection tank is refluxed to the sedimentation and selection tank for scouring of flocculent sludge to enrich granular sludge; Aerobic bacteria are distributed on the outer layer of the aerobic granular sludge, and anaerobic bacteria are distributed on the inner layer; Through the niche replacement of anaerobic ammonium oxidizing bacteria, promote the granulation of integrated shortcut nitrification anaerobic ammonium oxidation granular sludge based on the transformation of microbial niches.

2. The method for culturing integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge according to claim 1, characterized in that, The particle size of the aerobic granular sludge is 100 - 500 μm.

3. The method for culturing integrated short-cut nitrification anaerobic ammonium oxidation granular sludge according to claim 1, characterized in that, A part of the effluent from the sedimentation selection tank is recycled to achieve a liquid upward flow rate of 1.0 - 10.0 m / h in the sedimentation selection tank, promote the elutriation of light flocculent sludge, and enrich granular sludge.

4. A method for culturing integrated short-cut nitrification anaerobic ammonium oxidation granular sludge according to claim 1, characterized in that, Also comprising: Use a particle interception device to intercept the particles lost in the effluent and return them to the biological selection tank to reduce the loss of granular sludge.

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