Multi-station device for adding reagent into food detection sample

Abstract

The invention discloses a multi-station device for adding a reagent into a food detection sample, and particularly relates to the field of reagent adding, the device comprises a reagent cylinder, an opening and closing mechanism and an echelon liquid outlet control mechanism; the opening and closing mechanism comprises a liquid blocking block, a reset spring and a rotating seat; the rotating seat comprises a fixed rod, an arc-shaped transmission block, a rotating head and a connecting rod, one end of the fixed rod is fixed with the arc-shaped transmission block, the other end is fixed with the rotating head, the lower end of the reagent cylinder is rotatably connected with the rotating head, one end of the connecting rod is fixed with the rotating head, and the other end is fixed with the liquid blocking block; an arc-shaped chute matched with the movement path of the liquid blocking block is formed in the lower end of the reagent cylinder, one end of the reset spring is fixed with the chute wall of the arc-shaped chute, and the other end of the reset spring is fixed with the liquid blocking block; and the arc-shaped transmission block is in transmission connection with the echelon liquid outlet control mechanism. According to the device, different reagents can be periodically added into the sample liquid in sequence, so that the different reagents are mixed with the sample liquid in multiple batches, and the accuracy of the content of a target object to be detected is finally improved.

Description

Technical Field

[0001] The present application relates to the field of reagent addition, and in particular to a multi-functional device for adding reagents to food testing samples. Background Art

[0002] During the sample detection process, it is necessary to add different purification reagents, extraction reagents or derivatization reagents to the prepared samples. In the process of adding the purification reagents, for example, a pigment removal reagent, a lipid removal reagent or a reagent for removing a certain element is used. When the above different types of reagents are added to the sample in sequence, they are usually added to the sample liquid at one time on the premise of pre-preparing the volume. During the above operation, it is easy for the reagents and the sample liquid to be unevenly mixed, and part of the sample liquid is in a slurry state. Therefore, even if it is stirred immediately with a stirring rod, the various reagents cannot be dispersed into the sample liquid in the first time.

[0003] For example, Chinese invention patent CN2019112029179 discloses a test system and a method for quantitatively adding reagents, which realizes quantitative transfer of reagents by controlling a pipette gun with a robotic arm. However, in the process of preparing the analytical liquid on the machine, since there are many types of reagents to be used, but there is no strict requirement for the single liquid output, the above test system, although it realizes more intelligent liquid addition, is not suitable for the addition of multiple liquids. Summary of the invention

[0004] The present invention provides a device for adding reagents in a food test sample in a multi-station manner. The device can periodically add different reagents to the sample liquid in order, so that the reagents are mixed with the sample liquid in multiple batches, thereby ultimately improving the accuracy of the content of the target object to be tested.

[0005] The objective of the present invention is achieved through the following technical solutions: A multi-function device for adding reagents to food testing samples, comprising a reagent cartridge, an opening and closing mechanism, and a step-by-step liquid discharge control mechanism; The opening and closing mechanism includes a liquid blocking block, a return spring and a rotating seat; the rotating seat includes a fixed rod, an arc-shaped transmission block, a rotating head and a connecting rod, one end of the fixed rod is fixed to the arc-shaped transmission block, and the other end is fixed to the rotating head, the lower end of the reagent cylinder is rotatably connected to the rotating head, one end of the connecting rod is fixed to the rotating head, and the other end is fixed to the liquid blocking block; the lower end of the reagent cylinder is provided with an arc-shaped slide groove adapted to the movement path of the liquid blocking block, one end of the return spring is fixed to the groove wall of the arc-shaped slide groove, and the other end is fixed to the liquid blocking block; the arc-shaped transmission block is transmission-connected to the step-by-step liquid discharge control mechanism.

[0006] Among them, the step-by-step liquid discharge control mechanism controls the opening and closing mechanism to discharge liquid intermittently, thereby providing sufficient mixing time between different reagents and samples, which is conducive to the rapid diffusion of reagents into the sample liquid, thereby achieving the purpose of purifying or extracting the target object to be detected.

[0007] Preferably, the step-by-step liquid discharge control mechanism comprises a lower flip sheet, a transmission sheet, a vertical connecting rod, a transverse connecting rod, an upper flip sheet, a rod, a rotating shaft, a transmission plate and a periodic transmission assembly; The transmission plate is connected to the arc-shaped transmission block in a transmission manner, the transmission plate is fixed to the rotating shaft by a rod, the rotating shaft is fixed to the end of the upper flip plate; the lower flip plate and the upper flip plate are fixed at one end facing away from the rotating shaft; one end of the transmission plate rotates synchronously with the output end of the periodic transmission component, and the other end is rotationally connected to the vertical connecting rod, the end of the vertical connecting rod is fixed to the transverse connecting rod, and the end of the transverse connecting rod is rotationally connected to one end of the lower flip plate facing away from the upper flip plate.

[0008] Among them, the advantage of the cascade liquid output control mechanism is that it can control the addition of different types of reagents to the sample in sequence, reducing the participation of staff in non-creative activities and helping staff to get rid of frequent repetitive experimental work tasks.

[0009] Preferably, the transmission plate and the arc-shaped transmission block are both provided with meshing teeth.

[0010] Preferably, the step-by-step liquid discharge control mechanism also includes a load-bearing rod, the end of which is provided with a rotating shaft, one end of which is fixed to the lower flip plate, and the other end is fixed to the upper flip plate, and the upper flip plate and the lower flip plate are arranged at 180°.

[0011] Preferably, the periodic transmission assembly comprises a rotating wheel and a transmission wheel; The side end of the transmission wheel is provided with an extension part, the extension part is provided with a push column, the rotating wheel is provided with a groove adapted for the side end of the transmission wheel to be embedded in the rotation and a push groove adapted to the movement path of the push column, and the rotating wheel and the transmission sheet are synchronously connected in transmission.

[0012] Preferably, the device further comprises a liquid mixing cylinder for containing the mixed liquid and a rotary drive motor, wherein the rotary output end of the rotary drive motor is synchronously driven with the liquid mixing cylinder, and the drive wheel is synchronously driven with the rotary output end.

[0013] Preferably, a plurality of stirring blades are arranged around the circumference of the output end side.

[0014] Preferably, the device further comprises a fixing frame, wherein the fixing frame is provided with a positioning hole adapted for the reagent cartridge to be snapped in and fixed.

[0015] Preferably, the periodic transmission assembly also includes an L-shaped connecting rod, a positioning collar, a long rod and a mounting rod, one end of the long rod is fixed to the load-bearing rod, and the other end is fixed to the mounting rod, the free end of the mounting rod is screwed to the fixing frame, the positioning collar is sleeved on the connecting shaft where the rotating wheel and the transmission plate intersect, one end of the L-shaped connecting rod is fixed to the side end of the positioning collar, and the other end is fixed to the long rod.

[0016] Preferably, a fixing block is provided at the side end of the liquid mixing cylinder, and the upper end of the fixing block and the fixing frame are detachably fixed.

[0017] Compared with the prior art, the advantages or beneficial effects of the technical solution of the present application include: 1. By controlling different reagent cartridges to add reagents to the sample in sequence through the step-by-step liquid discharge control mechanism, the experimental efficiency can be greatly improved without manual operation. At the same time, some reagents are toxic, such as some derivatization reagents, and reducing manual contact is more conducive to protecting the personal safety of experimental personnel.

[0018] 2. The step-by-step liquid discharge control mechanism not only realizes the sequential addition of reagents, but also realizes the addition of reagents to the sample liquid in multiple batches, which is more conducive to the full mixing of reagents and sample liquid. For the extraction of target analytes, the advantages of this design can greatly improve the precipitation of target analytes. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the three-dimensional structure of a multi-functional device for adding reagents to food testing samples; Figure 2 It is a structural schematic diagram of the opening and closing mechanism and the step-by-step liquid discharge control mechanism; Figure 3 It is a schematic diagram of the structure of the opening and closing mechanism and the step-by-step liquid discharge control mechanism (the stirring blade is not shown); Figure 4 for Figure 3 A magnified view of part A in FIG. Figure 5 It is a structural schematic diagram of the opening and closing mechanism and the step-by-step liquid discharge control mechanism (top view); Figure 6 for Figure 5 A magnified view of part B in FIG. Figure 7 It is a structural schematic diagram of the periodic transmission component.

[0020] In the figure: 2, reagent cylinder, 3, fixing frame, 4, return spring, 5, fixing rod, 6, arc-shaped transmission block, 7, transmission plate, 8, rotating shaft, 9, liquid blocking block, 10, connecting plate, 11, upper flip plate, 12, stirring blade, 13, lower flip plate, 14, long rod, 140, mounting rod, 15, horizontal connecting rod, 16, L-shaped connecting rod, 17, rotating output end, 18, rotating wheel, 19, positioning collar, 20, transmission plate, 21, vertical connecting rod, 22, load-bearing rod, 23, rod, 24, groove, 25, push groove, 26, transmission wheel, 27, extension part, 28, push column, 31, fixing block, 32, mixing cylinder. DETAILED DESCRIPTION

[0021] The following will describe the implementation methods of the present application in detail with reference to the accompanying drawings and embodiments, so that the implementation process of how the present application applies technical means to solve technical problems and achieve corresponding technical effects can be fully understood and implemented accordingly. The embodiments of the present application and the various features in the embodiments can be combined with each other without conflict, and the technical solutions formed are all within the protection scope of the present application.

[0022] It should be clear that the embodiments described below are only part of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by technicians in the relevant field without making creative work are within the scope of protection of the present application. Example 1

[0023] A multi-function device for adding reagents to food testing samples comprises a reagent cartridge 2, an opening and closing mechanism and a step-by-step liquid discharge control mechanism.

[0024] The opening and closing mechanism includes a liquid blocking block 9, a reset spring 4 and a rotating seat; the rotating seat includes a fixed rod 5, an arcuate transmission block 6, a rotating head and a connecting rod, one end of the fixed rod 5 is fixed to the arcuate transmission block 6, and the other end is fixed to the rotating head, the lower end of the reagent cartridge 2 is rotatably connected to the rotating head, one end of the connecting rod is fixed to the rotating head, and the other end is fixed to the liquid blocking block 9; the lower end of the reagent cartridge 2 is provided with an arcuate chute adapted to the movement path of the liquid blocking block 9, one end of the reset spring 4 is fixed to the groove wall of the arcuate chute, and the other end is fixed to the liquid blocking block 9; the arcuate transmission block 6 is transmission-connected to the step-by-step liquid discharge control mechanism.

[0025] Connection relationship and working principle between opening and closing mechanism components: like Figure 2 As shown, when the rotating seat rotates counterclockwise (to the right), the blocking block 9 can slide down along the arc-shaped slide groove, and during the sliding process of the blocking block 9, the reset spring 4 is squeezed. At this time, the liquid outlet at the lower end of the reagent cartridge 2 is exposed, and the reagent in the reagent cartridge 2 will flow down along the liquid outlet.

[0026] When the rotating seat is not driven by the step-by-step liquid discharge control mechanism, the elastic recovery effect of the reset spring 4 causes the liquid blocking block 9 to return to its original position along the arc-shaped slide groove, and the liquid blocking block 9 will cover the liquid outlet again to stop the flow.

[0027] like Figure 2-Figure 4 As shown, the step-by-step liquid discharge control mechanism includes a lower flip plate 13, a transmission plate 20, a vertical connecting rod 21, a transverse connecting rod 15, an upper flip plate 11, a rod 23, a rotating shaft 8, a transmission plate 7, a load-bearing rod 22 and a periodic transmission assembly.

[0028] The transmission plate 7 is connected to the arc-shaped transmission block 6 by transmission, the transmission plate 7 is fixed to the rotating shaft by a rod 23, and the rotating shaft is fixed to the end of the upper flip sheet 11; the lower flip sheet 13 is fixed to one end of the upper flip sheet 11 facing away from the rotating shaft 8; one end of the transmission sheet 20 rotates synchronously with the output end of the periodic transmission component, and the other end is connected to the vertical connecting rod 21 by rotation, the end of the vertical connecting rod 21 is fixed to the transverse connecting rod 15, and the end of the transverse connecting rod 15 is connected to the end of the lower flip sheet 13 facing away from the upper flip sheet 11 by rotation. The transmission plate 7 and the arc-shaped transmission block 6 are both provided with meshing teeth. The end of the load-bearing rod 22 is provided with a rotating shaft, one end of the rotating shaft is fixed to the lower flip sheet 13, and the other end is fixed to the upper flip sheet 11, and the upper flip sheet 11 and the lower flip sheet 13 are arranged at 180°.

[0029] Among them, the periodic transmission component includes a rotating wheel 18 and a transmission wheel 26; an extension portion 27 is provided at the side end of the transmission wheel 26, and a push column 28 is provided at the extension portion 27. The rotating wheel 18 is provided with a groove 24 adapted to the side end of the transmission wheel 26 for embedding and rotating, and a push groove 25 adapted to the movement path of the push column 28. The rotating wheel 18 and the transmission plate 20 are synchronously transmitted and connected.

[0030] The working principle of the step-by-step liquid discharge control mechanism and the periodic transmission component is: like Figure 5-Figure 6 As shown in FIG. 1 , when the transmission wheel 26 is driven by the rotation drive motor, during the rotation process, when the arc-shaped portion of the transmission wheel 26 contacts the groove 24 of the rotating wheel 18, the rotating wheel 18 does not rotate, and when the push column 28 on the transmission wheel 26 slides in the push groove 25 of the rotating wheel 18, the rotating wheel 18 is driven to rotate. Figure 5 As shown, the number of the grooves 24 and the number of the push grooves 25 are both four, so the angle of each rotation of the rotating wheel 18 is 90°.

[0031] When the rotating wheel 18 rotates 90°, it will drive the transmission plate 20 fixed thereto to rotate 90° as well, and the transmission plate 20 will drive the vertical connecting rod 21 to rotate (the vertical position of the vertical connecting rod 21 changes). Figure 4-Figure 5When the transmission sheet 20 in the middle rotates, it will drive the transmission sheet 20 to rotate 90°, so that the lower end of the lower flip sheet 13 will rotate to the left or right (depending on the rotation direction of the transmission sheet 20), and because the upper end of the lower flip sheet 13 and the upper flip sheet 11 are fixed, the upper flip sheet 11 rotates 90° at the fixed point between it and the lower flip sheet 13, so the rotating shaft 8 fixed to the upper end of the upper flip sheet 11 will drive the transmission plate 7 to rotate. Figure 6 That is, the rotation of the transmission plate 7 drives the arc-shaped transmission block 6 to rotate, thereby controlling the liquid blocking block 9 to slide in the arc-shaped chute. When the transmission plate 7 slides to the next arc-shaped transmission block 6 and contacts it, it can also drive the liquid blocking block to slide in the arc-shaped chute. Figure 6 The figure shows three liquid discharge locations. The transmission plate 7 can be driven by the rotating wheel 18 to rotate clockwise and drive each arc-shaped transmission block on the motion path in turn.

[0032] In the above process, if Figure 2 In the embodiment, the design of the connecting plate 10 allows the upper flip pieces 11 on both sides to move synchronously. Since the rotating shaft 8 and the connecting plate 10 are rotatably connected, the upper flip piece 11 rotates. At the same time, a through slot is provided on the connecting plate 10, and the function of the through slot is to leave space for the transmission plate 7 to move. Figure 7 The middle is a schematic diagram showing the image of the step-by-step liquid discharge control mechanism (the through groove is not drawn on the connecting plate 10).

[0033] like Figure 1 As shown, this embodiment also includes a liquid mixing cylinder 32 for accommodating the mixed liquid and a rotation drive motor, the rotation output end 17 of the rotation drive motor is synchronously driven with the liquid mixing cylinder 32, and the transmission wheel 26 is synchronously driven with the rotation output end 17. A plurality of stirring blades 12 are arranged around the side end of the output end 17.

[0034] The rotating output end 17 can not only drive the transmission wheel 26 to rotate, but also drive the stirring blade 12 to mix the sample liquid and the reagent, thereby maximizing the homogenization efficiency.

[0035] This embodiment further comprises a fixing frame 3, and the fixing frame 3 is provided with a positioning hole adapted for the reagent cartridge 2 to be snapped in and fixed.

[0036] In this embodiment, the periodic transmission assembly also includes an L-shaped connecting rod 16, a positioning collar 19, a long rod 14 and a mounting rod 140. One end of the long rod 14 is fixed to the load-bearing rod 22, and the other end is fixed to the mounting rod 140. The free end of the mounting rod 140 is screwed to the fixing frame 3. The positioning collar 19 is sleeved on the connecting shaft where the rotating wheel 18 and the transmission plate 20 intersect. One end of the L-shaped connecting rod 16 is fixed to the side end of the positioning collar 19, and the other end is fixed to the long rod 14.

[0037] In this embodiment, a fixing block 31 is provided at the side end of the liquid mixing cylinder 32 , and the upper end of the fixing block 31 and the fixing frame 3 are detachably fixed. Example 2

[0038] In this embodiment, if Figure 1 As shown, four liquid adding sites are designed and arranged circumferentially on the liquid mixing cylinder 32. The purpose is to allow each reagent to be mixed with the sample liquid at the first time, rather than mixing the reagents with each other, so as to avoid chemical reactions between some reagents, thereby causing the effect on the sample to fail.

[0039] Figure 1 Although only four liquid addition sites are shown, when applied, the heating sites can be increased or decreased according to the actual type of reagent, so as to customize the liquid addition sites for a specific experiment, which is more conducive to high accuracy of the test results.

Claims

1. A multi-functional device for adding reagents to food test samples, characterized in that: It comprises a reagent cartridge (2), an opening and closing mechanism, and a step-by-step liquid discharge control mechanism; The opening and closing mechanism comprises a liquid blocking block (9), a return spring (4) and a rotating seat; The rotating seat comprises a fixed rod (5), an arc-shaped transmission block (6), a rotating head and a connecting rod, one end of the fixed rod (5) is fixed to the arc-shaped transmission block (6), and the other end is fixed to the rotating head, the lower end of the reagent cartridge (2) is rotatably connected to the rotating head, one end of the connecting rod is fixed to the rotating head, and the other end is fixed to the liquid blocking block (9); The lower end of the reagent cartridge (2) is provided with an arc-shaped slide groove adapted to the movement path of the liquid blocking block (9); one end of the return spring (4) is fixed to the groove wall of the arc-shaped slide groove, and the other end is fixed to the liquid blocking block (9); The arc-shaped transmission block (6) is in transmission connection with the step-by-step liquid discharge control mechanism.

2. A multi-station device for adding reagents to food test samples according to claim 1, characterized in that: The step-by-step liquid discharge control mechanism comprises a lower flip plate (13), a transmission plate (20), a vertical connecting rod (21), a transverse connecting rod (15), an upper flip plate (11), a rod (23), a rotating shaft (8), a transmission plate (7), and a periodic transmission assembly; The transmission plate (7) is transmission-connected to the arc-shaped transmission block (6); the transmission plate (7) and the rotating shaft are fixed via a rod (23); and the rotating shaft is fixed to the end of the upper flip plate (11); The lower flip plate (13) and the upper flip plate (11) are fixed at one end facing away from the rotating shaft (8); One end of the transmission plate (20) rotates synchronously with the output end of the periodic transmission component, and the other end is rotationally connected to the vertical connecting rod (21). The end of the vertical connecting rod (21) is fixed to the transverse connecting rod (15), and the end of the transverse connecting rod (15) is rotationally connected to the lower flip plate (13) with its back facing the upper flip plate (11).

3. A multi-station device for adding reagents to food test samples according to claim 2, characterized in that: The transmission plate (7) and the arc-shaped transmission block (6) are both provided with meshing teeth correspondingly.

4. The multi-station device for adding reagents to food test samples according to claim 2, characterized in that: The step-by-step liquid discharge control mechanism further comprises a load-bearing rod (22), the end of the load-bearing rod (22) being provided with a rotating shaft, one end of the rotating shaft being fixed to the lower flip sheet (13), and the other end of the rotating shaft being fixed to the upper flip sheet (11), the upper flip sheet (11) and the lower flip sheet (13) being arranged at 180 degrees.

5. The multi-station device for adding reagents to food testing samples according to claim 2, characterized in that: The periodic transmission assembly comprises a rotating wheel (18) and a transmission wheel (26); The side end of the transmission wheel (26) is provided with an extension portion (27), the extension portion (27) is provided with a push column (28), the rotating wheel (18) is provided with a groove (24) adapted to fit the side end of the transmission wheel (26) to be embedded and rotated, and a push groove (25) adapted to the movement path of the push column (28), the rotating wheel (18) and the transmission plate (20) are synchronously connected in transmission.

6. The multi-functional device for adding reagents to food testing samples according to claim 5, characterized in that: It also includes a liquid mixing cylinder (32) for containing the mixed liquid and a rotary drive motor, wherein the rotary output end (17) of the rotary drive motor is synchronously driven with the liquid mixing cylinder (32), and the drive wheel (26) is synchronously driven with the rotary output end (17).

7. The multi-station device for adding reagents to food testing samples according to claim 6, characterized in that: A plurality of stirring blades (12) are arranged around the circumference of the side end of the output end (17).

8. The multi-functional device for adding reagents to food test samples according to claim 6, characterized in that: It also comprises a fixing frame (3), wherein the fixing frame (3) is provided with a positioning hole adapted for the reagent cartridge (2) to be snapped in and fixed.

9. The multi-station device for adding reagents to food testing samples according to claim 8, characterized in that: The periodic transmission assembly further comprises an L-shaped connecting rod (16), a positioning collar (19), a long rod (14) and a mounting rod (140); one end of the long rod (14) is fixed to the load-bearing rod (22), and the other end is fixed to the mounting rod (140); the free end of the mounting rod (140) is screwed to the fixing frame (3); the positioning collar (19) is sleeved on a connecting shaft where the rotating wheel (18) and the transmission plate (20) intersect; one end of the L-shaped connecting rod (16) is fixed to the side end of the positioning collar (19), and the other end is fixed to the long rod (14).

10. The multi-station device for adding reagents to food testing samples according to claim 6, characterized in that: A fixing block (31) is provided at the side end of the liquid mixing cylinder (32), and the upper end of the fixing block (31) and the fixing frame (3) are detachably fixed.

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