Ultrahigh-speed centrifugal tube automatic puncher based on soil genome DNA (Deoxyribose Nucleic Acid) sample

Abstract

The invention belongs to the technical field of DNA sample detection, and particularly relates to an ultra-high-speed centrifugal tube automatic puncher based on a soil genome DNA sample. The puncher comprises a base supporting mechanism, a lifting driving mechanism and a punching mechanism, the lifting driving mechanism is arranged on the base supporting mechanism, the punching mechanism is connected with the lifting driving mechanism, a centrifugal tube to be punched is arranged at the top of the base supporting mechanism, and the lifting driving mechanism is used for driving the punching mechanism to ascend and descend. The punching mechanism is used for punching the bottom of the centrifugal tube to be punched. The centrifugal tube bottom punching device is simple and exquisite in structure, low in manufacturing cost and easy to popularize, solves the problem of vibration-free bottom punching of the centrifugal tube, and effectively avoids the problem that the bottom of the centrifugal tube is blocked and liquid drops cannot be discharged due to manual punching.

Description

Technical Field

[0001] The present invention belongs to the technical field of DNA sample detection, and in particular relates to an automatic ultra-high-speed centrifuge tube puncher based on soil genome DNA samples. Background Art

[0002] DNA-S IP is a technology that uses stable isotope labeling to reveal the functional microorganisms involved in the metabolic process of labeled substrates in complex environmental microbial communities. It uses stable isotopes such as 13C to label the substrate culture environment. The environmental microbial cells that use the labeled substrates continuously divide, grow, reproduce and synthesize 13C-DNA. The total DNA of the environmental microbial genome is extracted and the 13C-DNA is separated from the 12C-DNA by ultra-high-speed density gradient centrifugation. The 13C-DNA is further analyzed using molecular biology techniques, which will reveal the functional microorganisms that have assimilated the labeled substrates in complex environmental samples.

[0003] In the existing DNA-S IP process, after centrifugation in an ultra-high speed centrifuge, the labeled and unlabeled DNA in the sealed centrifuge tube will stay in different liquid layers respectively, and the DNA sample in the centrifuge tube has been stratified. In the current related articles and authorized patents, some multifunctional stratification instruments lack corresponding bottom punching instruments and can only be punched manually. The vibration generated can easily destroy the stratification of the sample in the tube, and the centrifuge tube fragments generated by manual punching can block the bottom of the centrifuge tube, which is easy to make mistakes and lead to experimental failure. Summary of the invention

[0004] In view of the above problems, the present invention aims to provide an automatic ultra-high speed centrifuge tube puncher based on soil genomic DNA samples to solve the problem of difficulty in punching holes at the bottom of DNA density gradient centrifuge tubes in current DNA-SIP experiments.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] The present invention provides an automatic ultra-high-speed centrifuge tube puncher based on soil genomic DNA samples, comprising a base support mechanism, a lifting drive mechanism and a punching mechanism, wherein the lifting drive mechanism is arranged on the base support mechanism, the punching mechanism is connected to the lifting drive mechanism, the centrifuge tube to be punched is arranged on the top of the base support mechanism, the lifting drive mechanism is used to drive the punching mechanism to lift and lower, and the punching mechanism is used to punch holes at the bottom of the centrifuge tube to be punched.

[0007] In a possible implementation, the base support mechanism includes a base, a column and a top plate, wherein the column is arranged on the base along a vertical direction, and the top plate is arranged on the top of the column.

[0008] In one possible implementation, the lifting drive mechanism includes a lead screw, a slide and a stepper motor, wherein the lead screw is rotatably installed between the top plate and the base and is parallel to the column, the slide is threadedly connected to the lead screw to form a threaded pair; the stepper motor is arranged on the top plate, and the output end is connected to the lead screw; the slide is slidably matched with the column; the punching mechanism is installed on the slide.

[0009] In one possible implementation, the punching mechanism includes an electric drill, a drill bit and a needle, wherein the electric drill is arranged on the slide along a vertical direction, and the needle is installed on the drill bit of the electric drill.

[0010] In a possible implementation, a centrifuge tube rack is provided on the top plate, and the centrifuge tube to be punched is installed on the centrifuge tube rack.

[0011] In a possible implementation, the automatic ultra-high-speed centrifuge tube puncher based on soil genomic DNA samples also includes a control system, which is used to control the lifting drive mechanism and the punching mechanism to perform the punching action.

[0012] The advantages and beneficial technical effects of the present invention are as follows: the present invention provides an automatic ultra-high-speed centrifuge tube puncher based on soil genome DNA samples, which has a simple and delicate structure, low manufacturing cost, and is easy to promote and popularize; it solves the problem of punching holes at the bottom of a centrifuge tube without vibration, and uses the automatic ultra-high-speed centrifuge tube puncher to punch holes at the bottom of a DNA centrifuge tube, which has the characteristics of simple operation, good repeatability and other advantages; and because the needle rotates at high speed to penetrate the bottom of the centrifuge tube, the debris particles generated by the drilling can be ground, effectively avoiding the problem of the bottom of the centrifuge tube being blocked and unable to produce droplets due to manual drilling.

[0013] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures particularly pointed out in the written description and the accompanying drawings.

[0014] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0016] Figure 1 This is a front view of an automatic ultra-high speed centrifuge tube puncher based on soil genomic DNA samples according to the present invention;

[0017] Figure 2It is a left view of an automatic ultra-high speed centrifuge tube puncher based on soil genomic DNA samples according to the present invention.

[0018] In the figure: 1. control system; 2. centrifuge tube to be punched; 3. needle; 4. base; 5. lead screw; 6. column; 7. slide; 8. stepper motor; 9. centrifuge tube rack; 10. electric drill; 11. drill bit. DETAILED DESCRIPTION

[0019] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0020] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0021] like Figure 1 , Figure 2 As shown, the present invention provides an automatic ultra-high-speed centrifuge tube puncher based on soil genomic DNA samples, comprising a base support mechanism, a lifting drive mechanism and a punching mechanism, wherein the lifting drive mechanism is arranged on the base support mechanism, the punching mechanism is connected to the lifting drive mechanism, the centrifuge tube 2 to be punched is arranged on the top of the base support mechanism, the lifting drive mechanism is used to drive the punching mechanism to lift and lower, and the punching mechanism is used to punch holes at the bottom of the centrifuge tube 2 to be punched.

[0022] Furthermore, the present invention provides an automatic ultra-high speed centrifuge tube puncher based on soil genomic DNA samples, which also includes a control system 1, and the control system 1 is used to control the lifting drive mechanism and the punching mechanism to perform the punching action.

[0023] In the embodiment of the present invention, the base support mechanism includes a base 4, a column 6 and a top plate, wherein the column 6 is arranged on the base 4 in a vertical direction, and the top plate is arranged on the top of the column 6. The lifting drive mechanism includes a lead screw 5, a slide 7 and a stepper motor 8, wherein the lead screw 5 is rotatably installed between the top plate and the base 4 and is parallel to the column 6, and the slide 7 is threadedly connected with the lead screw 5 to form a thread pair; the stepper motor 8 is arranged on the top plate, and the output end is connected with the lead screw 5; the slide 7 is slidably matched with the column 6; and the punching mechanism is installed on the slide 7. The stepper motor 8 is connected to the control system 1, and the stepper motor 8 drives the lead screw 5 to rotate, thereby driving the slide 7 and the punching mechanism thereon to lift and lower.

[0024] Furthermore, a centrifuge tube rack 9 is provided on the top plate, and the centrifuge tube 2 to be punched is installed on the centrifuge tube rack 9. The centrifuge tube rack 9 has a lock, which can stably fix the centrifuge tube 2 to be punched on the centrifuge tube rack 9.

[0025] In the embodiment of the present invention, the drilling mechanism includes an electric drill 10, a drill bit 11 and a needle 3, wherein the electric drill 10 is arranged on the slide 7 along the vertical direction, and the needle 3 is installed on the drill bit 11 of the electric drill 10. The electric drill 10 is connected to the control system 1, and the electric drill 10 drives the drill bit 11 and the needle 3 to rotate at a high speed.

[0026] The control system 1 contains a power supply and a controller. The stepper motor 8 and the electric drill 10 are connected to the control system 1 via cables, respectively, and the operating parameters of the stepper motor 8 and the electric drill 10 can be set.

[0027] The present invention provides an automatic ultra-high speed centrifuge tube puncher based on soil genomic DNA samples, and its working principle is:

[0028] The control system 1 is responsible for controlling the parameter setting and operation of the puncher. The centrifuge tube 2 to be punched of the DNA layered liquid sample after ultra-high-speed centrifugation is placed in the centrifuge tube rack 9 with a lock, and after being fixed, it is started by the control system 1. The stepper motor 8 drives the slide 7 through the screw 5 to drive the electric drill 10 to slide upward, and at the same time, the electric drill 10 drives the drill bit 11 to rotate at high speed, and the needle 3 on the top of the drill bit 11 pierces the centrifuge tube 2 to be punched directly below, realizing vibration-free automatic punching. After the current centrifuge tube 2 to be punched is completed, the punched centrifuge tube is removed, and the next centrifuge tube 2 to be punched can be replaced for the punching operation.

[0029] The present invention provides an automatic ultra-high-speed centrifuge tube puncher based on soil genomic DNA samples, which can automatically complete the vibration-free punching of the bottom of the sealed centrifuge tube, ensure the vibration-free punching process, and improve the accuracy and quality of the punching at the bottom of the centrifuge tube. The present invention has reasonable design, simple operation, low manufacturing cost, and is easy to promote and popularize. It can automatically punch holes at the bottom of the ultra-high-speed density gradient centrifuge DNA layered sample centrifuge tube without vibration, solving the problem of blockage of the bottom of the centrifuge tube and the lack of droplets due to manual punching.

[0030] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. An automatic ultra-high speed centrifuge tube puncher based on soil genomic DNA samples, characterized in that: It comprises a base support mechanism, a lifting drive mechanism and a punching mechanism, wherein the lifting drive mechanism is arranged on the base support mechanism, the punching mechanism is connected to the lifting drive mechanism, the centrifuge tube (2) to be punched is arranged on the top of the base support mechanism, the lifting drive mechanism is used to drive the punching mechanism to move up and down, and the punching mechanism is used to punch holes at the bottom of the centrifuge tube (2) to be punched.

2. The automatic ultracentrifuge tube puncher based on soil genomic DNA samples according to claim 1, characterized in that: The base support mechanism comprises a base (4), a column (6) and a top plate, wherein the column (6) is arranged on the base (4) in a vertical direction, and the top plate is arranged on the top of the column (6).

3. The automatic ultra-high speed centrifuge tube puncher based on soil genomic DNA samples according to claim 2, characterized in that: The lifting drive mechanism comprises a lead screw (5), a slide (7) and a stepper motor (8), wherein the lead screw (5) is rotatably mounted between the top plate and the base (4) and is parallel to the column (6), the slide (7) is threadedly connected to the lead screw (5) to form a thread pair; the stepper motor (8) is arranged on the top plate, and the output end is connected to the lead screw (5); the slide (7) is slidably matched with the column (6); and the punching mechanism is mounted on the slide (7).

4. The automatic ultracentrifuge tube puncher based on soil genomic DNA samples according to claim 3, characterized in that: The drilling mechanism comprises an electric drill (10), a drill bit (11) and a needle (3), wherein the electric drill (10) is arranged on the slide table (7) in a vertical direction, and the needle (3) is installed on the drill bit (11) of the electric drill (10).

5. The automatic ultra-high speed centrifuge tube puncher based on soil genomic DNA samples according to claim 2, characterized in that: A centrifuge tube rack (9) is provided on the top plate, and the centrifuge tube (2) to be punched is mounted on the centrifuge tube rack (9).

6. The automatic ultra-high speed centrifuge tube puncher based on soil genomic DNA samples according to claim 1, characterized in that: It also includes a control system (1), which is used to control the lifting drive mechanism and the punching mechanism to perform a punching action.

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