Two-photon laser-induced fluorescence collection system

Abstract

The invention belongs to the technical field of laser-induced fluorescence collection, and particularly discloses a two-photon laser-induced fluorescence collection system which comprises a laser, a photomultiplier, a refractor and a confocal lens, one end face of the refractor is a focusing face, and the other end face of the refractor is a turning face. The refractor is provided with a central through hole penetrating through the focusing surface and the turning surface, and the central through hole is horizontally arranged; laser emitted by the laser sequentially passes through the central through hole and the confocal lens and is focused by the confocal lens to generate fluorescent light, and the generated fluorescent light is received by the confocal lens, passes through the focusing surface and the refracting surface of the refractor and then is received by the photomultiplier. According to the invention, miniaturization of the two-photon laser-induced fluorescence system can be realized.

Description

Technical Field

[0001] The invention relates to the technical field of laser induced fluorescence collection, and in particular to a two-photon laser induced fluorescence collection system. Background Art

[0002] Two-photon laser-induced fluorescence diagnostic technology is usually used to measure the density, directional velocity and temperature of particles. This technology uses laser to excite ground state particles. The excited particles undergo a de-excitation process to a sub-energy level, and the sub-energy level transition produces fluorescence. The density, directional velocity and temperature parameters of the particles can be calculated by measuring and counting the wavelength of the emitted laser and the fluorescence intensity. Two-photon refers to the excitation of the same particle by two photons, which can increase the wavelength of the excitation laser and reduce the requirements for laser parameters. Two-photon laser-induced fluorescence diagnostic technology has important applications in the diagnosis of neutral particles in plasma, and can be used in the fields of controlled nuclear fusion, garbage disposal, chemistry and chemical engineering, chemistry and biology, etc. In order to adapt to the complex environmental conditions and space requirements around the controlled nuclear fusion device, the two-photon laser-induced fluorescence diagnostic system needs to be miniaturized to improve the system adaptability and operability. Summary of the invention

[0003] The invention provides a two-photon laser induced fluorescence collection system, aiming to realize the miniaturization of the two-photon laser induced fluorescence system or equipment.

[0004] The present invention is implemented by the following technical scheme: a two-photon laser induced fluorescence collection system, comprising a laser, a photomultiplier tube, a refractor and a confocal lens, wherein one end surface of the refractor is a focusing surface, and the other end surface of the refractor is a turning surface, and a central through hole penetrating the focusing surface and the turning surface is provided on the refractor, and the central through hole is arranged horizontally;

[0005] The laser emitted by the laser passes through the central through hole and the confocal lens in sequence, and generates fluorescence after being focused by the confocal lens. The generated fluorescence is received by the confocal lens, and after passing through the focusing surface and the refractive surface of the refractor, it is received by the photomultiplier tube.

[0006] Beneficial effects: In this scheme, the photomultiplier tube and the laser can be set on the same side, so that the laser can be incident and the fluorescence can be received from the same side. In this scheme, the fluorescence light path and the laser light path can be overlapped and merged through the refractor and the confocal lens, reducing the space occupied by the system, thereby effectively saving space, facilitating the reduction of the volume of the entire diagnostic system or diagnostic equipment, and realizing the miniaturization of the two-photon laser induced fluorescence system or equipment.

[0007] Furthermore, the refractor is in a cylindrical structure as a whole, the focusing surface of the refractor is a spherical structure, and the turning surface of the refractor is an oblique plane structure.

[0008] Beneficial effect: In this solution, the focusing surface of the refractor can gather fluorescence, and the turning surface of the refractor can turn and refract the fluorescence light path, so that the photomultiplier tube can receive it smoothly.

[0009] Furthermore, the refractor is entirely made of K9 optical glass.

[0010] Furthermore, the confocal lens includes an outer lens and an inner lens, both of which are convex lenses, and the inner lens is embedded inside the outer lens. The laser emitted by the laser can pass through the central through hole and the inner lens in sequence.

[0011] Beneficial effect: In this solution, the inner lens and the outer lens are integrated into one, which can reduce the occupied space and facilitate installation.

[0012] Furthermore, the inner lens is arranged opposite to the central through hole.

[0013] Beneficial effect: Such an arrangement enables the laser to vertically pass through the central refractor and the confocal lens, so that the laser light path will not be disturbed and deflected, thereby ensuring the collimated injection of the laser.

[0014] Furthermore, a mounting hole is coaxially opened in the middle of the outer lens, and the inner lens is embedded in the mounting hole.

[0015] Beneficial effect: The arrangement of the mounting hole in this solution facilitates the embedding of the inner lens and provides an installation space for the installation of the inner lens.

[0016] Furthermore, the mounting hole is a stepped hole.

[0017] Beneficial effect: In this solution, the mounting hole is set as a stepped hole, so that when the inner lens is installed, the stepped hole can play a role in accurately positioning the inner lens, thereby improving the installation accuracy of the inner lens. At the same time, the stepped hole can provide a support for the inner lens, making the installation of the inner lens more secure.

[0018] Furthermore, the inner lens is made of magnesium fluoride and is used to focus the laser, and the outer lens is made of K9 optical glass and is used to collect fluorescence.

[0019] Beneficial effects: This solution discloses the materials of the inner lens and the outer lens. In this solution, the inner lens and the outer lens can effectively focus the laser and collect fluorescence respectively.

[0020] Furthermore, a reflector is included, and the reflector can reflect and adjust the laser light emitted by the laser so that the laser light passes through the central through hole.

[0021] Beneficial effect: The addition of a reflector in this solution is conducive to adjusting the laser optical path according to factors such as the installation position and size of the laser, thereby improving operability in practical applications.

[0022] Furthermore, it also includes an optical fiber, which is connected to the photomultiplier tube, and the receiving end of the optical fiber is located at the focusing point of the fluorescence focused by the refractor.

[0023] Beneficial effect: The optical fiber added in this solution can place the photomultiplier tube away from the focusing point, which is suitable for environments with strong interference and where electrical devices need to be placed away from the focusing position.

[0024] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0025] The present invention utilizes a refracting mirror and a confocal lens, and can install a laser and a photomultiplier tube on the same side, so that laser light can be incident and fluorescence can be collected on the same side. The present invention combines the laser pipeline and the fluorescence light path through the refracting mirror and the confocal lens, reduces the space occupation of the system, realizes the overall miniaturization of the system and the equipment, and thus improves the adaptability and operability of the system.

[0026] Among them, the laser in the present invention is generated by a laser, passes through the central through hole of the refractor and the inner lens of the confocal lens, excites the ground state particles to the excited state at the focusing point, and the excited state particles are de-excited to generate fluorescence. The generated fluorescence passes through the outer lens of the confocal lens, the fluorescence focusing surface and the fluorescence turning surface of the refractor, and is finally received by the photomultiplier tube.

[0027] The central through hole of the refractor in the present invention is used to pass ultraviolet laser, the fluorescence focusing surface is a spherical structure for focusing fluorescence, and the fluorescence deflection surface is an oblique plane structure for refracting fluorescence. The inner lens of the confocal lens is made of magnesium fluoride for focusing laser, and the outer lens of the confocal lens is made of K9 optical glass for collecting fluorescence. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The drawings described herein are used to provide a further understanding of the embodiments of the present invention, constitute a part of this application, and do not constitute a limitation of the embodiments of the present invention. In the drawings:

[0029] Figure 1 Schematic diagram of the fluorescence refraction light path in an embodiment of a two-photon laser induced fluorescence collection system of the present invention Figure 1 ;

[0030] Figure 2 A stereoscopic diagram of a refraction mirror in an embodiment of a two-photon laser induced fluorescence collection system of the present invention;

[0031] Figure 3It is a front view of a refractor in an embodiment of a two-photon laser induced fluorescence collection system of the present invention;

[0032] Figure 4 It is a front view of a confocal lens in an embodiment of a two-photon laser induced fluorescence collection system of the present invention;

[0033] Figure 5 Schematic diagram of the fluorescence refraction light path in an embodiment of a two-photon laser induced fluorescence collection system of the present invention Figure 2 ;

[0034] Figure 6 Schematic diagram of the fluorescence refraction light path in an embodiment of a two-photon laser induced fluorescence collection system of the present invention Figure 3 .

[0035] Marks and corresponding parts names in the attached drawings:

[0036] Refraction mirror 1, focusing surface 1a, turning surface 1b, central through hole 1c, laser path 2, fluorescence path 3, laser focusing point 4, confocal lens 5, inner lens 5a, outer lens 5b, photomultiplier tube 6, laser 7, laser reflection mirror 8, optical fiber 9. DETAILED DESCRIPTION

[0037] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with embodiments and drawings. The exemplary embodiments of the present invention and their description are only used to explain the present invention and are not intended to limit the present invention.

[0038] like Figure 1 As shown, this embodiment provides a two-photon laser induced fluorescence collection system, including a laser 7, a photomultiplier tube 6, a refractor 1 and a confocal lens 5. In this embodiment, a two-photon laser induced fluorescence collection system is installed in a two-photon laser induced fluorescence diagnostic system or device.

[0039] Combination Figure 2 and Figure 3 As shown, one end face of the refractor 1 is a focusing surface 1a, and the other end face of the refractor 1 is a turning surface 1b. A central through hole 1c penetrating the focusing surface 1a and the turning surface 1b is provided on the refractor 1. The central through hole 1c is arranged horizontally, and in this embodiment, the refractor 1 is an overall cylindrical structure. The central through hole 1c is located at the center of the refractor 1 and is arranged coaxially with the refractor 1. The focusing surface 1a of the refractor 1 is a spherical structure, and the turning surface 1b of the refractor 1 is an oblique plane structure, that is, a spherical focusing surface 1a and an oblique turning surface 1b are respectively arranged at both ends of a cylinder, and the spherical focusing surface 1a of the refractor 1 forms a convex lens structure, which can focus the light path.

[0040] In this embodiment, the central through hole 1c is used to pass the ultraviolet laser, the fluorescence focusing surface 1a is a spherical structure for focusing the fluorescence, the fluorescence turning surface 1b is a beveled plane structure for refracting the fluorescence, and the refractor 1 is made of K9 optical glass. The focusing surface 1a is used to gather the fluorescence, but cannot refract the light path or make the light path turn, while the turning surface 1b can make the light path turn so that the fluorescence can be received by the photomultiplier tube 6.

[0041] The laser emitted by the laser 7 passes through the central through hole 1c and the confocal lens 5 in sequence, and generates fluorescence after being focused by the confocal lens 5. The generated fluorescence is received by the confocal lens 5, and after passing through the focusing surface 1a and the refractive surface of the refractor 1, it is received by the photomultiplier tube 6.

[0042] In this embodiment, Figure 4 As shown, the confocal lens 5 includes an outer lens 5b and an inner lens 5a. Both the outer lens 5b and the inner lens 5a are convex lenses. The inner lens 5a is embedded in the outer lens 5b. The laser emitted by the laser 7 can pass through the central through hole 1c and the inner lens 5a in sequence. In this embodiment, the inner lens 5a is arranged opposite to the central through hole 1c.

[0043] In this embodiment, a mounting hole is coaxially opened in the middle of the outer lens 5b. In this embodiment, the mounting hole is a stepped hole. The inner lens 5a is embedded in the mounting hole, and the mounting holes of the inner lens 5a and the outer lens 5b are bonded and reinforced, so that the combination of the inner lens 5a and the outer lens 5b is more stable and reliable. In this embodiment, the mounting hole is set as a stepped hole, which is more convenient for the installation and positioning of the inner lens 5a.

[0044] The inner lens 5a of the confocal lens is made of magnesium fluoride and is used to focus the laser, and the outer lens 5b of the confocal lens is made of K9 optical glass and is used to collect fluorescence.

[0045] In one embodiment, if Figure 1 As shown, the laser is generated by the laser 7, the laser path 2 passes through the central through hole 1c of the refractor 1, is focused by the inner lens 5a of the confocal lens 5, and excites the neutral particles at the laser focal point 4. The neutral particles transition to generate fluorescence, and the fluorescence path 3 of the generated fluorescence passes through the outer lens 5b of the confocal lens 5 and becomes parallel light. The parallel light passes through the refractor 1 and is focused on the photosensitive surface of the photomultiplier tube 6. The fluorescence intensity data is collected and recorded by the data sampling system. At this point, the two-photon laser induced fluorescence diagnostic system has completed the parameter measurement of neutral particles at the focal point.

[0046] Further, in another embodiment, Figure 5 As shown, a two-photon laser induced fluorescence collection system in this embodiment also includes a reflector, which can reflect and adjust the laser emitted by the laser 7 so that the laser passes through the central through hole 1c.

[0047] In the present invention, since it is necessary to satisfy that the laser emitted by the laser 7 can be perpendicular to the refractor 1, so that the laser pipeline can pass through the central through hole 1c horizontally, and when the size of the laser 7 is relatively large and it is inconvenient to adjust the position and angle of the laser 7, in this embodiment, the direction of the laser emitted by the laser 7 can be adjusted to the required position by adding a reflector. In the actual installation process, the number of reflectors can be adaptively selected according to factors such as the installation position and size of the laser 7. For example, in this embodiment, Figure 5 As shown, two sets of reflecting mirrors are used to adjust the laser light emitted by the laser 7 to be horizontally incident on the central through hole 1c.

[0048] In this embodiment, the laser is generated by the laser 7, reflected by two sets of reflectors, and then focused by the inner lens 5a of the confocal lens 5 through the central through hole 1c, and excited at the laser focal point 4. The neutral particles generate fluorescence through transition, and the fluorescence is converted into parallel light through the outer lens 5b of the confocal lens 5. The parallel light passes through the fluorescence refractor 1 and is focused on the photosensitive surface of the photomultiplier tube 6. The fluorescence intensity data is collected and recorded by the data sampling system. At this point, the two-photon laser induced fluorescence diagnostic system has completed a parameter measurement of neutral particles at the laser focal point 4. Compared with the above embodiment, this embodiment adds a set of reflectors for laser reflection, which is conducive to the adjustment of the laser optical path and can effectively improve the operability in practical applications.

[0049] Further, in another embodiment, Figure 6 As shown, a two-photon laser induced fluorescence collection system also includes an optical fiber 9, which is connected to a photomultiplier tube 6, and the receiving end of the optical fiber 9 is located at the focal point of the fluorescence focused by the refractor 1. In this embodiment, the laser is generated by a laser 7, passes through a central through hole 1c, and is focused by an inner lens 5a of a confocal lens 5, and excites neutral particles at the laser focal point 4. The neutral particles transition to generate fluorescence, and the fluorescence passes through an outer lens 5b of the confocal lens 5 and becomes parallel light. The parallel light passes through the fluorescence refractor 1 and is focused to the receiving end of the optical fiber 9. After passing through the optical fiber 9, the fluorescence reaches the photosensitive surface of the photomultiplier tube 6 from the outlet of the optical fiber 9, and the fluorescence intensity data is collected and recorded by the data sampling system. At this point, the two-photon laser induced fluorescence diagnostic system has completed a parameter measurement of neutral particles at the laser focal point 4. Compared with the above embodiment, this embodiment extends the fluorescence light path by adding an optical fiber 9, so that the photomultiplier tube 6 can be placed away from the focal point, which is suitable for environments with strong interference and where electrical devices need to be placed away from the focal position.

[0050] It should be noted that the above description of the disclosed embodiments enables professionals and technicians in the field to implement or use the present application. Various modifications to these embodiments will be apparent to professionals and technicians in the field, and the general principles defined herein can be implemented in other embodiments without departing from the scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but should conform to the widest scope consistent with the principles and novel features disclosed herein.

[0051] In the description of the present invention, it should be noted that the terms "first", "second", "third", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0052] In the description of this document, terms such as "up", "down", "left", "right", "front", "back", "top", "bottom", "inside", "outside", "middle", "vertical", "horizontal", "lateral", and "longitudinal" indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only used to illustrate the relative positional relationships between the various components or components, and do not particularly limit the specific installation orientations of the various components or components.

[0053] In the description of this document, some terms may be used to express other meanings in addition to indicating orientation or positional relationship. For example, the term "on" may also be used to express a certain dependency or connection relationship in some cases. For those of ordinary skill in the art, the specific meanings of these terms in this application can be understood according to the specific circumstances.

[0054] In the description of this document, the terms "installed", "set", "provided with", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection, or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be an internal connection between two devices, elements, or components. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0055] The structures, proportions, sizes, etc. drawn in the drawings in this application are only used to match the contents disclosed in this technical briefing book for technical personnel in this field to understand and read, and are not used to limit the restrictive conditions for the implementation of this application. Therefore, they have no substantive technical significance. Any structural modification, change in proportional relationship or adjustment of size, without affecting the efficacy and purpose that can be achieved by this application, should still fall within the scope of the technical content disclosed in this application.

[0056] The terms used in this document are those common terms currently widely used in the art in consideration of the functions of the present disclosure, but these terms may vary according to the intention of a person of ordinary skill in the art, precedents, or new technologies in the art. In addition, specific terms may be selected by the applicant, and in this case, their detailed meanings will be described in the detailed description of the present disclosure. Therefore, the terms used in the document should not be understood as simple names, but rather as an overall description based on the meaning of the terms and the present disclosure.

[0057] Flowcharts or text are used in this document to illustrate the operating steps performed according to the embodiments of the present application. It should be understood that the operating steps in the embodiments of the present application are not necessarily performed accurately in the order of recording. On the contrary, various steps can be processed in reverse order or simultaneously as needed. At the same time, other operations can also be added to these processes, or a certain step or several steps can be removed from these processes.

[0058] The above are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A two-photon laser induced fluorescence collection system, characterized in that: The invention comprises a laser, a photomultiplier tube, a refractor and a confocal lens, wherein one end surface of the refractor is a focusing surface, the other end surface of the refractor is a turning surface, a central through hole penetrating the focusing surface and the turning surface is provided on the refractor, and the central through hole is arranged horizontally; The laser emitted by the laser passes through the central through hole and the confocal lens in sequence, and generates fluorescence after being focused by the confocal lens. The generated fluorescence is received by the confocal lens, and after passing through the focusing surface and the refractive surface of the refractor, it is received by the photomultiplier tube.

2. A two-photon laser induced fluorescence collection system according to claim 1, characterized in that: The refractor is in a cylindrical structure as a whole, the focusing surface of the refractor is a spherical structure, and the turning surface of the refractor is an oblique plane structure.

3. A two-photon laser induced fluorescence collection system according to claim 1, characterized in that: The refractor is made entirely of K9 optical glass.

4. A two-photon laser induced fluorescence collection system according to claim 1, characterized in that: The confocal lens comprises an outer lens and an inner lens, both of which are convex lenses, and the inner lens is embedded in the outer lens. The laser light emitted by the laser can pass through the central through hole and the inner lens in sequence.

5. A two-photon laser induced fluorescence collection system according to claim 4, characterized in that: The inner lens is arranged opposite to the central through hole.

6. A two-photon laser induced fluorescence collection system according to claim 4, characterized in that: A mounting hole is coaxially opened in the middle of the outer lens, and the inner lens is embedded in the mounting hole.

7. A two-photon laser induced fluorescence collection system according to claim 6, characterized in that: The mounting hole is a stepped hole.

8. A two-photon laser induced fluorescence collection system according to any one of claims 1 to 7, characterized in that: The inner lens is made of magnesium fluoride and is used to focus the laser, and the outer lens is made of K9 optical glass and is used to collect fluorescence.

9. A two-photon laser induced fluorescence collection system according to any one of claims 1 to 7, characterized in that: It also includes a reflector, which can reflect and adjust the laser light emitted by the laser so that the laser light passes through the central through hole.

10. A two-photon laser induced fluorescence collection system according to any one of claims 1 to 7, characterized in that: It also includes an optical fiber, which is connected to the photomultiplier tube, and the receiving end of the optical fiber is located at the focusing point of the fluorescence focused by the refractor.

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