Method for grafting and seedling raising by reserving first true leaf of pumpkin rootstock

Abstract

The invention belongs to the technical field of grafting and seedling raising, and discloses a method for grafting and seedling raising by reserving a first true leaf of a pumpkin rootstock, which comprises the following steps: selecting the pumpkin rootstock; scion and stock seedling raising; reserving true leaves for grafting; grafted seedling healing period management; the specific operation of keeping the true leaves for attaching grafting is as follows: the true leaves and one of the cotyledons are separated to one side by the thumb and the index finger of the left hand, then a grafting blade disinfected by alcohol is used for beveling along the middle of the true leaves and a growing point, a notch is preferably used for simultaneously removing one of the cotyledons and the growing point, the other cotyledon is kept, and the notch is not too long and is about 1cm preferably; the scion is beveled by 30 degrees at the position 1-2 cm below the cotyledon base, it is guaranteed that the cut surface is smooth, and the length of the cut is about 1 cm. Grafted seedlings obtained through the method have higher root system related physiological indexes, grafting survival rate, SPAD value, plant height, stem diameter and leaf number, and healthy growth and strong seedling indexes of the seedlings are integrally improved.

Description

Technical Field

[0001] The invention belongs to the technical field of grafting seedling cultivation, and in particular relates to a method for grafting seedling cultivation by retaining the first true leaf of a pumpkin rootstock. Background Art

[0002] Since the 19th century, grafting, a traditional agronomic technique for improving crop resistance, has experienced rapid development. Among Cucurbitaceae crops, the number of grafted seedlings required in my country each year is calculated in units of 100 million. At present, when pumpkin is used as the rootstock for grafting, grafting, grafting, root cutting grafting and other grafting methods are generally used. When grafting with these grafting methods, the cotyledons and growth points of the rootstock are often discarded. In production, in order to reduce the occurrence of suckers, the growth point must be removed. Many companies choose to remove the first true leaf before grafting for the hypertrophy of the cotyledons, which undoubtedly increases the labor cost. Although removing the first true leaf improves the visual neatness and ventilation and light transmittance of the seedlings, their neatness for the scion does not depend on the presence or absence of the first true leaf. On the contrary, removing the true leaf adds another wound to the rootstock, resulting in an increase in wound fluid, which is not conducive to graft healing and the development of the root system during the healing period, especially for the seedlings grafted with root cutting.

[0003] The problems and defects of the prior art are: 1. Removing the first true leaf increases the labor input in the seedling production process; 2. Removing the first true leaf is not conducive to the root development of the rootstock; 3. The bleeding fluid of the rootstock is increased, which is not conducive to the healing of the grafted seedlings. Summary of the invention

[0004] In view of the problems existing in the prior art, the present invention provides a method for grafting and raising seedlings of pumpkin rootstocks by retaining the first true leaf.

[0005] The present invention is achieved by providing a method for grafting and raising seedlings of pumpkin rootstocks with the first true leaf retained, comprising the following steps:

[0006] S1, pumpkin rootstock selection;

[0007] S2, scion and rootstock seedling raising;

[0008] S3, grafting with retained true leaves;

[0009] S4, management of grafted seedlings during the healing period;

[0010] Furthermore, the rootstock selected in S1 is a pumpkin-type rootstock.

[0011] Furthermore, in S2, scion seeds and rootstock seeds with full grains are selected, and after being cleaned, they are soaked in warm water at about 55° C., cooled naturally to room temperature, and then soaked for about 8 hours.

[0012] Furthermore, in S2, the soaked seeds are drained of the soaking wastewater and transferred into a culture dish padded with moist gauze, and the culture dish is placed in an incubator at 28-30° C. for germination.

[0013] Furthermore, the seeds in the culture box in S2 are sown when they turn white, and a 32-hole plug tray is selected for mechanical sowing or manual sowing, and the scion can be sown using a tray.

[0014] Furthermore, in S3, after about 10 days, when the cotyledons of the scion are flattened, the rootstock has one leaf and one heart, and the first true leaf is just flattened, this first true leaf is retained for monocotyledon grafting, and water is applied the day before grafting to ensure the humidity of the substrate.

[0015] Furthermore, the specific operation of retaining true leaf grafting in S3 is: use the thumb and index finger of the left hand to separate the true leaf and one of the cotyledons to one side, and then use an alcohol-sterilized grafting blade to make an oblique cut along the middle of the true leaf and the growth point. The incision should be made to ensure that one cotyledon and the growth point are removed at the same time, leaving the other cotyledon. The incision should not be too long, about 1 cm is appropriate. The scion should be obliquely cut at 30° 1-2 cm below the base of the cotyledon to ensure a smooth cut surface. The incision length is about 1 cm, which can increase the matching degree with the rootstock incision and is conducive to grafting healing.

[0016] Further, in S3, the cut scion is fitted to the cut end of the rootstock and then fixed with a grafting clamp.

[0017] Further, the grafted seedlings completed in S4 are subjected to graft healing culture, and a certain closed humidification treatment is carried out in a stuffy culture box or with plastic film, with a daily temperature cycle of 28°C during the day and not less than 14°C at night; the relative humidity is about 95% for 2-5 days; the relative humidity is about 85% for 6-5 days; after the 10th day, routine management is carried out, and the relative humidity is about 70%; the first day after grafting, dark conditions are maintained, and the seedlings are covered with a sunshade net or a black film for shading treatment; from the 4th day to the 6th day, constant weak light, about 80 μmol·m -2 ·s -1 ; From the 7th to the 9th day, the light intensity increased to 170 μmol·m -2 ·s -1 ; After the 10th day, the normal light condition is about 300 μmol·m -2 ·s -1 The photoperiod is 14h day / 10h night, and transplanting can be carried out in about 14 days.

[0018] Another object of the present invention is to provide a method for grafting pumpkin rootstocks with the first true leaf retained for seedling cultivation, and the applicable crops include pumpkin-type rootstocks but are not limited to other crops of the Cucurbitaceae family and rootstocks of other families that can meet the requirements for cutting true leaves and growth points during grafting.

[0019] Furthermore, the grafting method is not limited to monocotyledon grafting and root-cut monocotyledon grafting.

[0020] In combination with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions to be protected by the present invention are as follows:

[0021] First, the present invention provides a method for grafting pumpkin rootstocks with the first true leaf retained, the core of which is to graft monocots without removing the first true leaf. This method can achieve the following improvements by retaining the first true leaf:

[0022] 1. Reduce labor costs: There is no need to remove the first true leaf before grafting, which greatly reduces labor input.

[0023] 2. Optimize root development: Maintaining the first true leaf can promote the normal growth of the root system of the rootstock, improve the physiological indicators of the root system, and ensure the healthy development of the root system during the grafting healing period, especially for root-breaking grafting.

[0024] 3. Reduce the production of bleeding sap: Since the first true leaf is no longer removed, the damage to the rootstock is reduced, which helps to reduce the secretion of bleeding sap and improve the healing effect of the grafted seedlings.

[0025] 4. The incision is conducive to grafting: the cutting part is not easy to cut out a cavity compared to the conventional method of removing the first true leaf for monocotyledon grafting, thereby increasing the area of ​​​​stock and scion fitting, which is conducive to grafting healing.

[0026] 5. Improve grafting survival rate and seedling index: Experimental results show that the grafted seedlings using the method of the present invention have higher root-related physiological indicators, grafting survival rate, SPAD value, plant height, stem thickness and number of leaves before planting, which improves the healthy growth and seedling index of the seedlings as a whole.

[0027] Second, the expected benefits and commercial value after the technical solution of the present invention is transformed as follows: the grafting method of the present invention does not require manual removal of true leaves before grafting, thereby reducing the input of labor. In agricultural production, reducing labor costs means saving a lot of money in large-scale seedling production. By reducing labor input, the saved labor costs can be invested in other production links to improve overall production efficiency. Due to the improved survival rate of grafted seedlings and the improved seedling index, higher quality seedlings can be provided to growers. This helps to improve the market competitiveness of commercial seedlings and bring higher economic benefits. From a commercial point of view, if this innovative grafting method is promoted, related technical services and supporting products (such as various stock seeds, scions and other means of production suitable for the grafting method) will also have greater market demand, creating more business opportunities.

[0028] The technical solution of the present invention fills the technical gap in the industry at home and abroad: the first true leaf is usually removed during the grafting of traditional pumpkin rootstocks, while the present invention proposes a method of retaining the first true leaf for grafting, which is a brand-new idea. In the existing research and practice of pumpkin rootstock grafting technology, no similar complete technical solution for grafting seedlings with retaining the first true leaf as the core has been found. This method adds new content to the field of pumpkin rootstock grafting technology.

[0029] The technical solution of the present invention solves the technical problems that people have been eager to solve but have never been successful: people have always faced the problems of high labor input, affected root system development, increased bleeding fluid that is not conducive to the healing of grafted seedlings, and easy appearance of cavities when cutting the stock during pumpkin rootstock grafting. Previous attempts may have been limited by technical ideas, and the expectations for grafted seedlings were not ideal. The present invention, starting from the perspective of retaining the first true leaf, solves these technical problems that have troubled people for a long time to a certain extent.

[0030] The technical solution of the present invention overcomes technical prejudice: in traditional concepts, people may think that removing the first true leaf is a necessary step in the pumpkin rootstock grafting process. This concept has existed for a long time and has affected people's research and practice directions, and is also a convention in the industry. The present invention breaks through this technical prejudice and proves that retaining the first true leaf will not only not affect the grafting effect, but can bring many benefits, such as improving the grafting survival rate and seedling index, etc., opening up a new direction for the development of pumpkin rootstock grafting technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic diagram of true leaf retention grafting provided by an embodiment of the present invention;

[0032] Figure 2 It is a physical flow chart of retaining true leaf grafting provided by an embodiment of the present invention;

[0033] Figure 3 Schematic diagram of stock cutting provided by an embodiment of the present invention; A is a stock cutting operation diagram; B is a stock cutting effect diagram;

[0034] Figure 4 It is a comparative diagram of the root system development of pumpkin with true leaves retained and root grafted and with true leaves removed provided by an embodiment of the present invention;

[0035] Figure 5 14 days after grafting; A, root system development diagram with the first true leaf retained; B, root system development diagram with the first true leaf removed; C, root system scan with the first true leaf removed; D, root system scan with the first true leaf retained. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0037] The selection of pumpkin rootstock is the first key step to the success of grafting seedlings. Usually, pumpkin varieties that are strong, disease-free, resistant to adversity and have good growth potential are selected as rootstocks. This not only provides sufficient nutrients for the scion, but also improves the disease resistance of the grafted seedlings. When selecting the rootstock, it is also necessary to ensure that the seedling age is moderate. Too young or too old rootstocks will affect the grafting survival rate.

[0038] During the seedling raising stage, the scion and rootstock must be grown synchronously to ensure that their growth and development are coordinated. Before sowing, the soaking treatment of the rootstock and scion seeds, temperature and humidity control, and lighting conditions should be strictly controlled to ensure that the seedlings are uniform and strong. In addition, the rootstock and scion should maintain a moderate water supply during the seedling raising process to avoid excessive dryness or wetness, so as to facilitate the healthy development of the seedlings.

[0039] Step S3 is the "retain true leaves" method. First, use the thumb and index finger of your left hand to gently separate the true leaf and one of the cotyledons to avoid hurting the plant. Next, use an alcohol-sterilized grafting blade to make an oblique cut between the true leaf and the growth point. The length of the incision should be kept at about 1 cm, ensuring that one cotyledon and growth point are removed while retaining the other cotyledon. This operation not only helps to reduce the wound area, but also reduces water evaporation and improves the grafting survival rate.

[0040] Cutting the scion also requires skill. Usually, the scion needs to be cut at a 30° angle 1-2 cm below the base of the cotyledons. This can provide a smoother contact surface, making the contact between the scion and the rootstock closer, thus ensuring good healing ability. It is best to control the incision length to about 1 cm, which can ensure sufficient contact surface without damaging other parts of the scion.

[0041] After the incision is completed, grafting is an important step. By accurately matching the cut surfaces of the rootstock and scion, it can ensure that their cambium layers can be well integrated. Grafting should be done gently and quickly to prevent the plant from being exposed to the air for too long and losing water. At the same time, the grafted seedlings should be fixed with grafting clips to ensure that the scion and rootstock are stable and not loose, so as to facilitate later healing and growth.

[0042] After the grafting is completed, the management of the healing period is particularly important. During this stage, appropriate temperature, humidity and light conditions should be provided. Usually, maintaining a temperature of around 25°C and high humidity will help promote healing. In the early stages of healing, direct exposure to strong light should be avoided, and the graft should be gradually transitioned to normal light conditions. The first three days after grafting are the critical period for healing, and proper environmental control can effectively improve the success rate of grafting.

[0043] After successful healing, the grafted seedlings gradually resume growth and enter the normal management stage. At this time, it is necessary to strengthen water management and keep the soil moist but not waterlogged. In addition, the grafted seedlings need to be applied with an appropriate amount of nitrogen fertilizer or other nutrients to promote the healthy growth of the seedlings. Check the growth of the plants regularly and remove yellow or dead leaves in time to ensure that the grafted seedlings remain healthy.

[0044] The grafting seedling raising method significantly improves the stress resistance and survival rate of the grafted seedlings. The method has broad application prospects in agricultural production, especially in the grafting seedling raising process of vegetables such as pumpkin and cucumber. Reasonable grafting operation can not only improve production efficiency, but also effectively reduce the occurrence of diseases, increase crop yield and quality, and play a positive role in promoting modern agriculture.

[0045] Embodiment 1:

[0046] In this embodiment, the selected experimental material rootstock Z5 is Cucurbita moschata, a crop of the Cucurbitaceae family, which is bred by the research group, and the scion is muskmelon "k8-45" provided by the Hami Melon Research Center of Xinjiang Academy of Agricultural Sciences. The selected rootstock is a pumpkin-type rootstock. The growth point of this type of rootstock is just at the base of the cotyledon during grafting. Therefore, when grafting, only one cut is needed to remove a cotyledon and the growth point, which is convenient for grafting and cutting without increasing the incision area ( Figure 3 ).

[0047] The embodiment of the present invention provides a method for grafting pumpkin rootstock with the first true leaf retained, comprising the following steps:

[0048] (1) Selection of pumpkin rootstock and scion: Choose pumpkin seeds Z5 and melon seeds k8-45 with full grains.

[0049] (2) Raising scion and rootstock seedlings: Wash the scion seeds and rootstock seeds with clean water, soak them in warm water at about 55°C after washing, so as to disinfect pathogens and improve the germination rate and growth quality of seeds. Naturally cool to room temperature and soak for about 8 hours. Drain the soaked seeds and transfer them to a culture dish lined with moist gauze. Put the culture dish into an incubator at 28-30°C for germination. When the seeds in the incubator turn white, sow them. Select a 32-hole tray for mechanical sowing or manual sowing. The scion can be sown using a tray.

[0050] (3) Grafting with retained true leaves: After sowing, wait for about 10 days until the cotyledons of the scion are flattened and the stock has one leaf and one heart. When the first true leaf is just flattened, retain this first true leaf for monocot grafting. Water the substrate one day before grafting to ensure the humidity of the substrate. The specific operation of retaining true leaves is: use the thumb and index finger of the left hand to move the true leaf and one of the cotyledons to one side, and then use a grafting blade disinfected with alcohol to make an oblique cut along the middle of the true leaf and the growth point. The incision should ensure that one cotyledon and the growth point are removed at the same time, leaving the other cotyledon. The incision should not be too long, about 1 cm is appropriate. The scion should be obliquely cut at 30° 1-2 cm below the base of the cotyledon to ensure a smooth cut surface. The incision length is about 1 cm, which can increase the matching degree with the stock incision and facilitate grafting healing. Then fit the cut scion to the stock incision and fix it with a grafting clip. Figure 1 ).

[0051] (4) Management of grafted seedlings during the healing period: The completed grafted seedlings are cultured for grafting healing, and a sealed humidification treatment is performed using a stuffy box or plastic film. The daily temperature cycle is 28°C during the day and not less than 14°C at night; the relative humidity is about 95% for 2-5 days; the relative humidity is about 85% for 6-5 days; after the 10th day, routine management is carried out, and the relative humidity is about 70%; the first day after grafting, the dark conditions are maintained, and the seedlings are covered with a sunshade net or black film for shading treatment; from the second day to the sixth day, the light is kept constant, about 80 μmol·m -2 ·s -1 ; From the 7th to the 9th day, the light intensity increased to 170 μmol·m -2 ·s -1 ; After the 10th day, the normal light condition is about 300 μmol·m -2 ·s -1 The photoperiod is 14h day / 10h night, and transplanting can be carried out in about 14 days.

[0052] (5) Experimental results: The results showed that pumpkin rootstocks could significantly improve the survival rate, SPAD value, plant height, stem thickness, fresh weight, leaf area and seedling index of grafted seedlings while retaining the first true leaf for monocotyledon grafting, which was beneficial to the cultivation of strong seedlings (Table 1).

[0053] Table 1 Comparison of grafting with retained true leaves and grafting with removed true leaves

[0054]

[0055]

[0056] (Note: Seedling index = (stem diameter of scion / plant height) * root surface area; *, **, **, ** represent significant differences at P < 0.03, 0.002, 0.0002, 0.0001 levels. Same below)

[0057] Embodiment 2:

[0058] In this embodiment, the selected experimental material stock is pumpkin stock Z16, which is a high-generation inbred line bred by this research group, and the scion is thick-skinned melon "k8-45", which is the same as in Example 1.

[0059] (1) The specific implementation steps of pumpkin rootstock and scion selection, scion and rootstock seedling cultivation are the same as those in Example 1.

[0060] (2) Grafting with retained true leaves: The first true leaf is retained and the monocotyledon is grafted. The cutting of the stock and scion and the grafting method are the same as in Example 1. The only difference is that after the grafting is completed, the plant needs to be cut obliquely from the surface of the substrate to form a section of about 30°, and then the cut end is inserted into a hole tray with moist substrate in advance, and the cutting depth is about 2 cm.

[0061] (3) After the grafting is completed, the management of the healing period is the same as in Example 1.

[0062] (4) Experimental results: The results showed that the survival rate, SPAD value, plant height, stem diameter, fresh weight, leaf area and seedling index of pumpkin rootstocks can be significantly improved under the premise of retaining the first true leaf for monocotyledonous root grafting, which is conducive to the cultivation of strong seedlings (Table 2). Figure 5 (A and D are grafted with the first true leaf retained; B and C are controls with the first true leaf removed) are photos taken 14 days after grafting.

[0063] Table 2 Comparison of grafting with true leaves and removing true leaves

[0064]

[0065] The invention is applied to the field of vegetable grafting seedling cultivation, especially melon seedling cultivation industry. In the field of seedling production, it can be applied to factory seedling cultivation enterprises and seedling cultivation experiments of scientific research institutions. The former can reduce costs and improve the competitiveness of seedling quality, while the latter is helpful for scientific research.

[0066] The test results are shown in Table 1 and Table 2: The data shown in both are collected at the same time point on the 14th day after grafting. Except for the overall statistics of survival rate, each indicator sample was repeated for no less than 30 biological replicates. The significance analysis was performed using the t-test. If there is no "*", it means there is no significant difference. First of all, the survival rate. The two embodiments use different grafting methods, and both show the unique advantage of retaining the first true leaf for the successful healing of the grafted seedlings, especially for root-cut grafting, as shown in Example 2, the survival rate of the grafted seedlings and the uniformity of the grafted seedlings are improved. Figure 4As shown. In the two embodiments, the survival rate, SPAD value, plant height, stem thickness, fresh weight, and leaf area of ​​the grafting method with the first true leaf retained are superior, and the strong seedling index is significantly improved compared with the grafting method with the first true leaf removed, which is conducive to the cultivation of strong seedlings. The effect of the method of the present invention is more obvious when the first true leaf is retained and the root grafting is performed. Figure 5 -A, the development of the root system and the compactness of the root soil are significantly better than those of the control 5-B. Similarly, the results of 5-C and 5-D scanned by the root scanner after root washing also reflect the importance of retaining the first true leaf graft for root regeneration.

[0067] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with the technical field within the technical scope disclosed by the present invention and within the spirit and principle of the present invention should be covered by the protection scope of the present invention.

Claims

1. A method for grafting pumpkin rootstock with the first true leaf retained, characterized in that: By selecting strong pumpkin rootstocks and scions, the seedlings are raised simultaneously; during the grafting process, one true leaf of the pumpkin rootstock is retained and the growth point and the other cotyledon are removed; the oblique surfaces of the scion and the rootstock are tightly attached and fixed with grafting clips; and suitable temperature, humidity and light conditions are provided during the healing period to ensure that the grafted seedlings heal quickly and grow normally.

2. The method for grafting and raising seedlings of pumpkin stock with the first true leaf retained as claimed in claim 1, characterized in that: The following steps are involved: S1, pumpkin rootstock selection; S2, scion and rootstock seedling raising; S3, grafting with retained true leaves; S4, management of grafted seedlings during the healing period; The specific operation of retaining true leaf grafting in S3 is: use the thumb and index finger of the left hand to separate the true leaf and one of the cotyledons to one side, and then use a grafting blade disinfected with alcohol to make an oblique cut along the middle of the true leaf and the growing point. The incision should be sufficient to ensure that one cotyledon and the growing point are removed at the same time, leaving the other cotyledon. The incision should not be too long, about 1 cm is appropriate. The scion should be obliquely cut at 30° 1-2 cm below the base of the cotyledon to ensure a smooth cut surface. The incision length is about 1 cm.

3. The method for grafting and raising seedlings of pumpkin stock with the first true leaf retained as claimed in claim 1, characterized in that: The rootstock used in S1 is a pumpkin-type rootstock; in S2, scion seeds and rootstock seeds with full grains are selected, and after being cleaned, they are soaked in warm water at about 55°C, naturally cooled to room temperature, and then soaked for about 8 hours.

4. The method for grafting and raising seedlings of pumpkin stock with the first true leaf retained as claimed in claim 1, characterized in that: In S2, the soaked seeds are drained of the soaking wastewater and transferred into a culture dish lined with moist gauze, and the culture dish is placed in an incubator at 28-30° C. for germination.

5. The method for grafting and raising seedlings of pumpkin stock with the first true leaf retained as claimed in claim 1, characterized in that: The seeds in the incubator in S2 are sown when they turn white. A 32-hole plug tray is selected for mechanical sowing or manual sowing. The scion can be sown using a tray.

6. The method for grafting and raising seedlings of pumpkin stock with the first true leaf retained as claimed in claim 1, characterized in that: In S3, wait for about 10 days until the cotyledons of the scion are flattened, the rootstock has one leaf and one heart, and the first true leaf is just flattened. Keep this first true leaf for monocot grafting. Water the graft one day before grafting to ensure the humidity of the substrate.

7. The method for grafting and raising seedlings of pumpkin stock with the first true leaf retained as claimed in claim 1, characterized in that: In S3, the cut scion is fitted onto the cut end of the rootstock and then fixed with a grafting clamp.

8. The method for grafting and raising seedlings of pumpkin stock with the first true leaf retained as claimed in claim 1, characterized in that: The grafted seedlings completed in S4 are cultured for graft healing, and a sealed humidification treatment is performed in a stuffy box or with plastic film. The daily temperature cycle is 28°C during the day and not less than 14°C at night; the relative humidity is about 95% for 2-5 days; the relative humidity is about 85% for 6-5 days; after the 10th day, routine management is carried out, and the relative humidity is about 70%; the first day after grafting, dark conditions are maintained, and covered with a sunshade net or black film for shading treatment; from the 4th to the 6th day, constant weak light, about 80μmol·m -2 ·s -1 ; From the 7th to the 9th day, the light intensity increased to 170 μmol·m -2 ·s -1 ; After the 10th day, the normal light condition is about 300 μmol·m -2 ·s -1 The photoperiod is 14h day / 10h night, and transplanting can be carried out in about 14 days.

9. An application of the method for grafting and raising seedlings by retaining the first true leaf of pumpkin rootstock as claimed in any one of claims 1 to 8 in grafting and raising seedlings, characterized in that: Applicable crops include pumpkin-type rootstocks but are not limited to other crops of the Cucurbitaceae family and rootstocks of other families that can meet the cutting requirements of true leaves and growing points during grafting.

10. Application of the method for grafting and raising seedlings by retaining the first true leaf of pumpkin rootstock as claimed in claim 9 in grafting and raising seedlings, characterized in that: Grafting methods are not limited to monocotyledon grafting and root-cut monocotyledon grafting.

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