Essential oil compound and application thereof in preparation of antibacterial deodorant

Abstract

The invention belongs to the technical field of antibiosis and deodorization, and particularly discloses an essential oil compound and application thereof in preparation of an antibacterial deodorant. According to the research, low-concentration lime essential oil and zinc sulfate have relatively weak antibacterial effects on standard strains and drug-resistant strains, and after the lime essential oil and the zinc sulfate are combined, an obvious synergistic antibacterial effect is achieved. The traditional Chinese medicine composition has a more efficient bacteriostatic effect and can be used instead of antibiotics. In addition, the deodorization effect also has a synergistic effect, and the two components are easy to obtain and safe under low-dosage use, and have a relatively good application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of antibacterial deodorant products, and in particular relates to an essential oil compound and application thereof in preparing an antibacterial deodorant. Background Art

[0002] Lime essential oil is distilled from lime cones. It has a strong smell, bitter and sweet, and is light yellow. The main components of lime essential oil are terpenes, coriander alcohol, terpineol, etc. It has anti-scorbutic, antibacterial, and antiviral effects.

[0003] Zinc ions can precipitate proteins and have astringent and antiseptic effects when applied externally, but they have no antibacterial activity. There are not many studies on the combined bactericidal effects of essential oils and compounds reported, so it is of research and practical significance to study compounds that can have a combined bactericidal effect with lime essential oil.

[0004] Existing chemical deodorants have high cost and large usage volume, and they also pose great environmental risks. Essential oils are also used in the field of deodorization. For example, animal husbandry, factories, sewage treatment, landfills, hospitals and other environments also face the need for deodorization. Therefore, the development of green, low-risk, and low-cost deodorants also has strong practical significance. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides an essential oil compound.

[0006] Another object of the present invention is to provide the use of the essential oil complex in the preparation of an antibacterial deodorant.

[0007] The purpose of this application is achieved through the following technical solutions:

[0008] An essential oil complex consisting of zinc sulfate and lime essential oil.

[0009] Preferably, in the essential oil complex, the concentration of lime essential oil is 1%-5%, and the concentration of zinc sulfate is 25-100 μg / mL.

[0010] An antibacterial and deodorizing product comprises the essential oil complex.

[0011] Preferably, the object of bacteriostasis is one or more of E. coli BW 25113, ATCC 29213 or drug-resistant E. coli strain NP109; and the object of deodorization is ammonia water.

[0012] Preferably, water is used as the solvent, and the mass concentration of the lime essential oil is 1% to 5%.

[0013] The present invention also provides a combined sterilization method, wherein lime essential oil and zinc sulfate are incubated with bacterial solution at the same time, the incubation temperature is 37°C, and the incubation time is 1 hour. Preferably, the bacterial solution concentration is 106 Preferably, the bacteriostatic object is one or more of E. coli BW 25113, ATCC 29213 or drug-resistant E. coli strain NP109.

[0014] The invention also provides a deodorizing method using lime essential oil in combination with zinc sulfate, wherein the lime essential oil and zinc sulfate are diluted and then directly sprayed for use.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention combines the provided natural product lime essential oil with zinc sulfate, and finds that the combination of lime essential oil and zinc sulfate has a significant bactericidal effect on Escherichia coli BW 25113. The synergistic effect of lime essential oil and zinc sulfate can reduce the use of high-concentration lime essential oil while ensuring the bactericidal effect. The above effect is also applicable to inhibiting Staphylococcus aureus and drug-resistant Escherichia coli. The deodorizing effect on ammonia water shows that the combination of lime essential oil and zinc sulfate significantly enhances the deodorizing effect. The two components are used in low amounts, are safe and effective, and the raw materials are easily available, and have good application prospects in preparing antibacterial and deodorizing products. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The statistical result diagram of the antibacterial effect in Example 2;

[0018] Figure 2 The statistical result diagram of the antibacterial effect in Example 3;

[0019] Figure 3 This is a statistical result diagram of the antibacterial effect in Example 4. DETAILED DESCRIPTION

[0020] The specific embodiments of the present invention below will provide a detailed and comprehensive description of the technical solutions of the present invention. It should be noted that the embodiments provided represent only a part of the present invention, not all of it. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods; the materials, reagents, etc. used are reagents and materials that can be obtained from commercial channels unless otherwise specified.

[0022] Lime essential oil (LM) is a product of Guangzhou Wuyuan Essential Oil Co., Ltd.; zinc sulfate (ZnSO4) is a Yongda Reagent brand, MW287.55.

[0023] Judgment principle: If the bacterial count in the experimental group is reduced by more than 0.5 logarithmic values ​​on the basis of the decrease in the bacterial count in the treatment with lime essential oil alone and zinc sulfate alone, it indicates that lime essential oil and zinc sulfate have a synergistic antibacterial effect.

[0024] Example 1 MIC determination of lime essential oil and zinc sulfate against Escherichia coli BW 25113, Staphylococcus aureus ATCC 29213 and multidrug-resistant strain NP109

[0025] 1. Experimental materials:

[0026] (1) Experiment: Cool the autoclaved MH broth for later use.

[0027] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.

[0028] Zinc sulfate: Yongda Reagent brand, MW287.55

[0029] Test strains: wild strain of Escherichia coli BW 25113; quality control strain of Staphylococcus aureus ATCC 29213; multidrug-resistant Escherichia coli NP109 (laboratory collection).

[0030] 2. Preparation before the test:

[0031] (1) The wild-type Escherichia coli strain BW 25113, the quality control strain of Staphylococcus aureus ATCC 29213, and the multidrug-resistant Escherichia coli NP109 were subcultured on agar medium and cultured to an appropriate size.

[0032] (2) Using a precision balance, weigh 0.0561 g of zinc sulfate powder in a centrifuge tube, add 10 mL of sterile water to dissolve it, vortex to mix the zinc sulfate concentration and filter it. Pass the filtrate through a 0.22 μm diameter filter membrane for physical sterilization, divide it into sterilized centrifuge tubes, and store it in a -20°C freezer.

[0033] 3. MIC determination experiment:

[0034] (1) Inoculate Escherichia coli BW 25113, Staphylococcus aureus ATCC 29213, and Escherichia coli multidrug-resistant strain NP109 in a centrifuge tube containing 4 mL of MH broth, place in a shaker at 37°C and shake at 180 rpm for 4 h, and then remove the centrifuge tube;

[0035] (2) Use MH broth to dilute the incubated bacteria to 100 times, about 10 6 CFU / mL, reserve;

[0036] (3) Take a sterile 96-well plate, add 180 μL of MH broth medium to well 1, and add 100 μL of MH broth medium to wells 2-12;

[0037] (4) Add 20 μL of lime essential oil or 20 μL of zinc sulfate (5120 μg / mL) to the first well, pipette and mix well, then pipette 100 μL into the second well, and so on, pipette 100 μL into the tenth well and discard;

[0038] (5) Add 100 μL of diluted bacterial solution to wells 1 to 11 and 200 μL of MH broth to well 12;

[0039] (6) The final concentrations (%) of lime essential oil in wells 1-10 of a 96-well plate were 5%, 2.5%, 1.25%, 0.625%, 0.3125%, 0.156%, 0.078%, 0.039%, 0.0195%, and 0.00975%, well 11 was a growth control, and well 12 was a blank control;

[0040] (7) The final concentrations of zinc sulfate (μg / mL) in wells 1-10 of a 96-well plate were 256, 128, 64, 32, 16, 8, 4, 2, 1, and 0.5, well 11 was a growth control, and well 12 was a blank control;

[0041] (8) Set up 3 replicates for each drug;

[0042] (9) Place the inoculated 96-well plate in a 37°C incubator and incubate for 16-18 hours, then read the results.

[0043] The results are shown in Table 1. The MIC value of BW 25113 lime essential oil is >5%, and the MIC value of zinc sulfate is >256 μg / mL; the MIC value of ATCC29213 lime essential oil is >5%, and the MIC value of zinc sulfate is 256 μg / mL; the MIC value of NP109 lime essential oil is >5%, and the MIC value of zinc sulfate is >256 μg / mL.

[0044] Table 1 MIC of lime essential oil and zinc sulfate against standard bacteria and resistant bacteria

[0045]

[0046] Example 2 Evaluation of the combined bactericidal effect of lime essential oil and zinc sulfate on Escherichia coli BW 25113

[0047] 1. Experimental materials:

[0048] (1) Experiment: Cool the autoclaved LB broth, PBS buffer, and ultrapure water for later use.

[0049] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.

[0050] Zinc sulfate: Yongda Reagent brand, MW287.55

[0051] Test strain: Escherichia coli wild strain BW 25113 (laboratory collection).

[0052] 2. Preparation before the test:

[0053] (1) The wild-type Escherichia coli strain BW 25113 was subcultured on agar medium and cultured to an appropriate size.

[0054] (2) Use a precision balance to weigh 0.0561 g of zinc sulfate powder in a centrifuge tube, add 10 mL of sterile water to dissolve it, and the zinc sulfate concentration is 10 mg / mL. Vortex mix and filter. Pass the filtrate through a 0.22 μm diameter filter membrane for physical sterilization, divide it into sterilized centrifuge tubes, and store it in a -20°C freezer.

[0055] 3. Sterilization experiment:

[0056] (1) Inoculate a single colony of Escherichia coli BW 25113 in a centrifuge tube containing 20 mL of LB broth, place the tube in a shaker at 37°C and shake at 180 rpm for 3 h, then remove the tube.

[0057] (2) Centrifuge the cultured bacterial solution at 5500 rpm for 3 min, resuspend in an equal volume of PBS buffer and dilute 10-fold. 7 CFU / mL, for future use.

[0058] (3) This experiment uses a 1 mL system and adds the bacterial solution in (2):

[0059] A) Control group: 0.7 mL PBS + 0.2 mL PBS + 0.1 mL bacterial solution;

[0060] B) Single drug group: 0.7 mL PBS + 0.1 mL A / B drug + 0.1 mL PBS + 0.1 mL bacterial solution;

[0061] C) Combined group: 0.7 mL bacterial solution + 0.1 mL A drug + 0.1 mL B drug + 0.1 mL bacterial solution.

[0062] (4) This test set up 9 groups:

[0063] Escherichia coli BW25113: ①Control group; ②Lime essential oil group: 1MIC (5%) lime essential oil; ③Lime essential oil group: 1 / 4MIC (1.25%) lime essential oil; ④Zinc sulfate group: 25μg / mL zinc sulfate; ⑤Zinc sulfate group: 100μg / mL zinc sulfate; ⑥Combination group: 1MIC lime essential oil + 25μg / mL zinc sulfate; ⑦Combination group: 1MIC lime essential oil + 100μg / mL zinc sulfate; ⑧Combination group: 1 / 4MIC lime essential oil + 25μg / mL zinc sulfate; ⑨Combination group: 1 / 4MIC lime essential oil + 100μg / mL zinc sulfate;

[0064] (5) After the system is prepared, incubate the culture at 37°C with shaking at 180 rpm for 1 h.

[0065] (6) At 1 h, 100 μL of bacterial solution was aspirated from each tube and added to a 2 ml centrifuge tube containing 900 μL PBS buffer for ten-fold gradient dilution. After dilution, 25 μL was dropped on LB agar medium and incubated in a 37°C incubator for 16 to 18 h. The colonies were counted and the experimental results were statistically analyzed after three biological replicates.

[0066] The results are shown in Table 2 and Figure 1 As shown, the bacteria in the blank control group grew normally, indicating that Escherichia coli BW 25113 could grow normally under the experimental conditions; in the single-drug group, there was no significant decrease in Escherichia coli BW 25113 compared with the ZnSO4 group alone, indicating that the inhibitory effect of the ZnSO4 group alone on Escherichia coli BW 25113 was not obvious; the same results also showed that the use of high-concentration lime essential oil alone had a significant inhibitory effect on Escherichia coli BW 25113; the results of the combined group showed that the synergistic effect of lime essential oil and zinc sulfate had a significant bactericidal effect on Escherichia coli BW 25113.

[0067] Table 2 Bactericidal effect of lime essential oil and zinc sulfate on wild strain of Escherichia coli BW 25113

[0068]

[0069] Example 3 Evaluation of the combined bactericidal effect of lime essential oil and zinc sulfate on Staphylococcus aureus ATCC 29213

[0070] 1. Experimental materials:

[0071] (1) Experiment: Cool the autoclaved LB broth, PBS buffer, and ultrapure water for later use.

[0072] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.

[0073] Zinc sulfate: Yongda Reagent brand, MW287.55

[0074] Test strain: Staphylococcus aureus quality control strain ATCC 29213 (laboratory collection).

[0075] 2. Preparation before the test:

[0076] (1) Subculture the Staphylococcus aureus quality control strain ATCC 29213 on agar medium and grow to an appropriate size.

[0077] (2) Use a precision balance to weigh 0.0561 g of zinc sulfate powder in a centrifuge tube, add 10 mL of sterile water to dissolve it, and the zinc sulfate concentration is 10 mg / mL. Vortex mix and filter. Pass the filtrate through a 0.22 μm diameter filter membrane for physical sterilization, divide it into sterilized centrifuge tubes, and store it in a -20°C freezer.

[0078] 3. Sterilization experiment:

[0079] (1) Inoculate a single colony of Staphylococcus aureus ATCC 29213 in a centrifuge tube containing 20 mL of LB broth, place the tube in a shaker at 37°C and shake at 180 rpm for 3 h, then remove the tube.

[0080] (2) Centrifuge the cultured bacterial solution at 5500 rpm for 3 min, resuspend in an equal volume of PBS buffer and dilute 10-fold. 7 CFU / mL, for future use.

[0081] (3) This experiment uses a 1 mL system and adds the bacterial solution in (2):

[0082] A) Control group: 0.7 mL PBS + 0.2 mL PBS + 0.1 mL bacterial solution;

[0083] B) Single drug group: 0.7 mL PBS + 0.1 mL A / B drug + 0.1 mL PBS + 0.1 mL bacterial solution;

[0084] C) Combined group: 0.7 mL bacterial solution + 0.1 mL A drug + 0.1 mL B drug + 0.1 mL bacterial solution.

[0085] (4) This test set up 9 groups:

[0086] Staphylococcus aureus ATCC 29213: ① control group; ② lyme essential oil group: 1MIC (5%) lyme essential oil; ③ lyme essential oil group: 1 / 4MIC (1.25%) lyme essential oil; ④ zinc sulfate group: 25μg / mL zinc sulfate; ⑤ zinc sulfate group: 100μg / mL zinc sulfate; ⑥ combined medication group: 1MIC lyme essential oil + 25μg / mL zinc sulfate; ⑦ combined medication group: 1MIC lyme essential oil + 100μg / mL zinc sulfate; ⑧ combined medication group: 1 / 4MIC lyme essential oil + 25μg / mL zinc sulfate; ⑨ combined medication group: 1 / 4MIC lyme essential oil + 100μg / mL zinc sulfate;

[0087] (5) After the system is prepared, incubate the culture at 37°C with shaking at 180 rpm for 1 h.

[0088] (6) At 1 h, 100 μL of bacterial solution was aspirated from each tube and added to a 2 ml centrifuge tube containing 900 μL PBS buffer for ten-fold gradient dilution. After dilution, 25 μL was dropped on LB agar medium and incubated in a 37°C incubator for 16 to 18 h. The colonies were counted and the experimental results were statistically analyzed after three biological replicates.

[0089] The results are shown in Table 3 and Figure 2 As shown, the bacteria in the blank control group grew normally, indicating that Staphylococcus aureus ATCC 29213 could grow normally under the experimental conditions; in the single-drug group, there was no significant decrease in Staphylococcus aureus ATCC 29213 compared with the single ZnSO4 group, indicating that the inhibitory effect of the single ZnSO4 group on Staphylococcus aureus ATCC 29213 was not obvious; the same results also showed that the use of high-concentration lime essential oil alone had a significant inhibitory effect on Staphylococcus aureus ATCC 29213; the results of the combined group showed that the synergistic effect of lime essential oil and zinc sulfate had a significant bactericidal effect on Staphylococcus aureus ATCC 29213.

[0090] Table 3 Bactericidal effect of lime essential oil and zinc sulfate on Staphylococcus aureus quality control strain ATCC 29213

[0091]

[0092]

[0093] Example 4 Evaluation of the combined bactericidal effect of lime essential oil and zinc sulfate on multidrug-resistant Escherichia coli NP109

[0094] 1. Experimental materials:

[0095] (1) Experiment: Cool the autoclaved LB broth, PBS buffer, and ultrapure water for later use.

[0096] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.

[0097] Zinc sulfate: McLean brand, MW169.87

[0098] Test strain: multidrug-resistant Escherichia coli NP109 (laboratory collection).

[0099] 2. Preparation before the test:

[0100] (1) The multidrug-resistant Escherichia coli NP109 was subcultured on agar medium and cultured to an appropriate size.

[0101] (2) Use a precision balance to weigh 0.0561 g of zinc sulfate powder in a centrifuge tube, add 10 mL of sterile water to dissolve it, and the zinc sulfate concentration is 10 mg / mL. Vortex mix and filter. Pass the filtrate through a 0.22 μm diameter filter membrane for physical sterilization, divide it into sterilized centrifuge tubes, and store it in a -20°C freezer.

[0102] 3. Sterilization experiment:

[0103] (1) Inoculate a single colony of multidrug-resistant Escherichia coli NP109 in a centrifuge tube containing 20 mL of LB broth, place the tube in a 37°C shaker at 180 rpm for 3 h, and then remove the tube.

[0104] (2) Centrifuge the cultured bacterial solution at 5500 rpm for 3 min, resuspend in an equal volume of PBS buffer and dilute 10-fold. 7 CFU / mL, for future use.

[0105] (3) This experiment uses a 1 mL system and adds the bacterial solution in (2):

[0106] A) Control group: 0.7 mL PBS + 0.2 mL PBS + 0.1 mL bacterial solution;

[0107] B) Single drug group: 0.7 mL PBS + 0.1 mL A / B drug + 0.1 mL PBS + 0.1 mL bacterial solution;

[0108] C) Combined group: 0.7 mL bacterial solution + 0.1 mL A drug + 0.1 mL B drug + 0.1 mL bacterial solution.

[0109] (4) This test set up 9 groups:

[0110] Escherichia coli resistant bacteria NP109: ① control group; ② Lyme essential oil group alone: ​​1MIC (5%) Lyme essential oil; ③ Lyme essential oil group alone: ​​1 / 2MIC (2.5%) Lyme essential oil; ④ Zinc sulfate group alone: ​​25μg / mL zinc sulfate; ⑤ Zinc sulfate group alone: ​​100μg / mL zinc sulfate; ⑥ Combination group: 1MIC Lyme essential oil + 25μg / mL zinc sulfate; ⑦ Combination group: 1MIC Lyme essential oil + 100μg / mL zinc sulfate; ⑧ Combination group: 1 / 2MIC Lyme essential oil + 25μg / mL zinc sulfate; ⑨ Combination group: 1 / 2MIC Lyme essential oil + 100μg / mL zinc sulfate;

[0111] (5) After the system is prepared, incubate the culture at 37°C with shaking at 180 rpm for 1 h.

[0112] (6) At 1 h, 100 μL of bacterial solution was aspirated from each tube and added to a 2 ml centrifuge tube containing 900 μL PBS buffer for ten-fold gradient dilution. After dilution, 25 μL was dropped on LB agar medium and incubated in a 37°C incubator for 16 to 18 h. The colonies were counted and the experimental results were statistically analyzed after three biological replicates.

[0113] The results are shown in Table 4 and Figure 3 As shown, bacteria in the blank control group grew normally, indicating that the multidrug-resistant Escherichia coli NP109 could grow normally under the experimental conditions; in the single-drug group, there was no significant decrease in the multidrug-resistant Escherichia coli NP109 compared with the single ZnSO4 group, indicating that the inhibitory effect of the single ZnSO4 group on the multidrug-resistant Escherichia coli NP109 was not obvious; the same results also showed that the use of lime essential oil alone had a significant inhibitory effect on the multidrug-resistant Escherichia coli NP109; the results of the combined group showed that the synergistic effect of lime essential oil and zinc sulfate had a significant bactericidal effect on the multidrug-resistant Escherichia coli NP109, and the bactericidal rate of the combined group was at least doubled compared with the single-drug group.

[0114] Table 4 Bactericidal effect of lime essential oil and zinc sulfate on multidrug-resistant Escherichia coli NP109

[0115]

[0116]

[0117] Example 5 Evaluation of the deodorizing effect of lime essential oil combined with zinc sulfate

[0118] 1. Experimental materials:

[0119] (1) Test: Prepare a portable gas detector and a sealed aluminum box for use.

[0120] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.

[0121] Zinc sulfate: Yongda Reagent brand, MW287.55

[0122] The test simulates the odor of a farm: ammonia.

[0123] 2. Preparation before the test:

[0124] (1) Debug the portable gas detector and complete the self-test.

[0125] 3. Deodorization experiment:

[0126] (1) Turn on the gas detector, put it into the detection box after debugging, and wait for 3 minutes to ensure that the instrument is stable.

[0127] (2) In this experiment, the dosage of ammonia water is 10 μL, and the liquid is dripped on the fixed position of the detection box:

[0128] A) Control group: 10 μL ammonia water;

[0129] B) Single drug group: 10 μL ammonia water + 1 μL diluted single drug;

[0130] C) Combination group: 10 μL ammonia water + 1 μL diluted drug A + 1 μL diluted drug B.

[0131] (3) This experiment set up 9 groups:

[0132] Ammonia: ①Control group; ②Lime essential oil group: 1MIC (5%) lime essential oil; ③Lime essential oil group: 1 / 4MIC (1.25%) lime essential oil; ④Zinc sulfate group: 25μg / mL zinc sulfate; ⑤Zinc sulfate group: 100μg / mL zinc sulfate; ⑥Combination group: 1MIC lime essential oil + 25μg / mL zinc sulfate; ⑦Combination group: 1MIC lime essential oil + 100μg / mL zinc sulfate; ⑧Combination group: 1 / 4MIC lime essential oil + 25μg / mL zinc sulfate; ⑨Combination group: 1 / 4MIC lime essential oil + 100μg / mL zinc sulfate;

[0133] (4) After the liquid is added, start the test and record the test value every 3 minutes;

[0134] (5) Result judgment: 0-19 means below the warning line, no alarm; 20-49 means low-level alarm; 50-100 means high-level alarm (this value directly represents the high and low concentration data values ​​of ammonia degradation).

[0135] The results are shown in Table 5. The detection value in the blank control group was 100, indicating that the odor detection was stable under the experimental conditions. The results of the single-drug group and the combined group showed that 30 minutes after the use of lime essential oil alone, the detection value of the high-concentration group was below the warning line, and the other single-drug groups were all in the low-level alarm range. The combined effect of lime essential oil and zinc sulfate on ammonia water was significant. After 27 minutes, all the combined treatment groups were below the warning line. The high-concentration lime essential oil combined group reached below the warning line in 21 minutes, indicating that the combined use of lime essential oil and zinc sulfate had a significant synergistic deodorizing effect.

[0136] Table 5 Deodorization effect of lime essential oil and zinc sulfate on ammonia water

[0137]

[0138] Obviously, the above embodiments of the present invention are only examples for clearly illustrating the technical solution of the present invention, and are not intended to limit the specific implementation methods of the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the claims of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. An essential oil compound, characterized in that: Composed of zinc sulfate and lime essential oil.

2. The essential oil compound according to claim 1, characterized in that In the essential oil complex, the concentration of lime essential oil is 1%-5%, and the concentration of zinc sulfate is 25-100 μg / mL.

3. An antibacterial and deodorizing product, characterized in that: The invention comprises the essential oil compound according to claim 1 or 2.

4. The antibacterial and deodorizing product according to claim 3, characterized in that: The object of inhibition is one or more of E. coli BW 25113, ATCC 29213 or drug-resistant E. coli strain NP109.

5. The antibacterial and deodorizing product according to claim 3, characterized in that: The object of deodorization is ammonia water.

6. The antibacterial and deodorizing product according to claim 3, characterized in that: Water is used as the solvent, and the mass concentration of the lime essential oil is 1% to 5%.

7. A method for sterilizing with lime essential oil and zinc sulfate, characterized in that: Lime essential oil and zinc sulfate were incubated with the bacterial solution at the same time, the incubation temperature was 37°C, and the incubation time was 1 hour.

8. The method according to claim 7, characterized in that The bacterial solution concentration is 10 6 CFU / mL.

9. A method for deodorizing by using lime essential oil in combination with zinc sulfate, characterized in that: Dilute lime essential oil and zinc sulfate and spray directly on the skin.

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