Automatic cell slide preparation equipment

Abstract

The invention discloses automatic cell slide preparation equipment. The automatic cell slide preparation equipment comprises a shell provided with a slide preparation cavity; the environment adjusting mechanism is assembled on the shell and is used for adjusting the environment parameters of the flaking cavity; the consumable mechanism is arranged in the film making cavity and is used for accommodating film making consumables; the incubation mechanism is arranged in the slide preparation cavity and is used for bearing a slide; the dropping mechanism is used for moving between the consumable mechanism and the incubation mechanism, and the drying fan is connected with the dropping mechanism. In the application, the environmental parameters are adjusted through the environmental adjusting mechanism, so that the film preparation cavity has the environmental parameters required by the experiment process, and meanwhile, the film dropping mechanism is used for taking and placing consumables and dropwise adding a solution, so that the experiment dosage and operation are standardized, the consistency and repeatability are effectively kept, and the chemical or physical properties of a specimen are kept in a stable state; and air circulation is accelerated through the drying fan, the slide preparation efficiency is improved, and the stability of cell slide preparation is ensured, so that a clear and stable cell image is obtained.

Description

Technical Field

[0001] The present application relates to the technical field of specimen analysis, and in particular to an automatic cell preparation device. Background Art

[0002] Chromosome specimen preparation and karyotype analysis are currently the basic technical means for clinical genetic diagnosis. However, since the preparation of chromosome specimens is susceptible to multiple variables, how to stably obtain metaphase chromosomes with good morphology, clear structure, appropriate length, good dispersion, and sufficient quantity is still a problem often encountered by many basic genetics laboratories. In reality, the operation techniques of each experimental operator are not uniform, and the surrounding environmental parameters are constantly changing, making it difficult to ensure that the current environmental parameters are the environmental parameters required for the experimental process, which will greatly increase the difficulty of experimental repeatability and the failure rate of the experiment in the drop stage, seriously affecting the success rate and production efficiency of chromosome specimen preparation. Summary of the invention

[0003] In order to overcome at least one of the defects of the prior art described above, the present application provides an automatic cell preparation device, which can adjust the environmental parameters of the preparation chamber, standardize the experimental dosage and experimental operation, and effectively maintain the consistency and repeatability of the experimental process.

[0004] An automatic cell preparation device according to an embodiment of the present application includes: a shell body, which is provided with a preparation chamber; an environmental adjustment mechanism, which is assembled on the shell body and is used to adjust the environmental parameters of the preparation chamber; a consumable mechanism, which is arranged in the preparation chamber and is used to accommodate preparation consumables; an incubation mechanism, which is arranged in the preparation chamber and is used to carry glass slides; a drop film mechanism, which is used to move between the consumable mechanism and the incubation mechanism, and is capable of taking and placing consumables when the drop film mechanism moves to the consumable mechanism, and is capable of dropping solution onto the glass slide when the drop film mechanism moves to the incubation mechanism; and a drying fan, which is connected to the drop film mechanism and can move with the drop film mechanism, and is used to accelerate the air circulation at the glass slide after the solution is dropped.

[0005] In the automatic cell preparation equipment, the environmental parameters of the preparation chamber are adjusted by the environmental adjustment mechanism so that the preparation chamber has the environmental parameters required by the experimental process. At the same time, the dropper mechanism is used to take and place consumables and drop solutions, standardize experimental dosage and experimental operation, so that each batch can be carried out on the parameters required by the experimenter, effectively maintain the consistency and repeatability of the experimental process and the chemical or physical properties of the specimens in a stable state, greatly improve the success rate of chromosome specimen preparation, and the drying fan can accelerate the air circulation at the glass slide after the solution is added, promote rapid drying of the preparation, improve the preparation efficiency, ensure the quality and stability of cell preparation, thereby obtaining clearer and more stable cell images, and thus improve the accuracy of testing and analysis methods.

[0006] According to some embodiments of the present application, the film drop mechanism includes a film drop rack and a film drop assembly, the film drop rack is movably connected in the film making chamber, the film drop assembly is movably connected to the film drop rack, the drying fan is connected to the film drop assembly, and the drying fan can move along with the film drop assembly.

[0007] According to some embodiments of the present application, the drop film assembly includes an identification unit and a pipette gun, the pipette gun is movably connected to the drop film rack, and the identification unit and the drying fan are both connected to the pipette gun.

[0008] According to some embodiments of the present application, it also includes a guide cover and a heating assembly; the air inlet of the guide cover is connected to the air outlet of the drying fan, and the air outlet of the guide cover faces the glass slide; the heating assembly is assembled on the sample table, and the glass slide is placed on the heating assembly.

[0009] According to some embodiments of the present application, a cleaning mechanism is also included, which includes a liquid adding needle and a liquid outlet needle; the liquid outlet needle is assembled on the drop film rack to absorb the cleaning waste liquid; the liquid outlet needle is connected to the drop film assembly and can move with the drop film assembly to release the cleaning liquid.

[0010] According to some embodiments of the present application, the incubation mechanism includes an incubation bracket and a sample table; the incubation bracket is arranged in the preparation cavity, an incubation cabin is provided inside the incubation bracket, and a storage space is formed between the lower end of the incubation bracket and the inner wall of the preparation cavity, the consumable mechanism is slidably arranged in the storage space, and the consumable mechanism can extend out of or be accommodated in the storage space; the sample table is slidably arranged in the incubation cabin for carrying glass slides, and the sample table can carry glass slides and extend out of or be accommodated in the incubation cabin.

[0011] According to some embodiments of the present application, the consumable mechanism includes a consumable workbench, a pipette rack, a sample tube placement rack, a waste liquid trough and a consumable disposal trough, the consumable workbench is slidably arranged in the storage space, and the pipette rack, the sample tube placement rack, the waste liquid trough and the consumable disposal trough are arranged on the consumable workbench.

[0012] According to some embodiments of the present application, the environmental conditioning mechanism includes at least one heat exchange component; the heat exchange component includes a heat exchange fan, a heat exchange plate and a heat exchange box, the air outlet of the heat exchange box is connected to the film making chamber, and the heat exchange fan is arranged between the heat exchange plate and the air outlet of the heat exchange box.

[0013] According to some embodiments of the present application, a humidification component is also included, which includes an atomization box, an air pump and an air outlet pipe. The air inlet of the air pump is connected to the film making chamber, the atomization box is connected to the air outlet of the air pump, and the air outlet pipe connects the atomization box with the heat exchange box.

[0014] According to some embodiments of the present application, an exhaust component is also included, which includes an exhaust pipe, a check box, a check cover and an exhaust fan. A valve plate is provided on the side of the check box close to the film-making chamber, and the valve plate is provided with an exhaust opening, and the exhaust opening connects the film-making chamber with the check box; the side of the check cover close to the film-making chamber abuts against the side of the valve plate away from the film-making chamber, and the upper end of the check cover is rotatably connected to the valve plate or the check box; the exhaust pipe is provided on the side of the check box away from the film-making chamber, and the exhaust fan connects the exhaust pipe with the check box.

[0015] In summary, the automatic cell preparation device provided by this application has the following technical effects: The environmental parameters of the slide preparation chamber are adjusted by the environmental adjustment mechanism so that the slide preparation chamber has the environmental parameters required by the experimental process. At the same time, the slide dropping mechanism is used to take and place consumables and drop solutions, standardize experimental dosage and experimental operation, so that each batch can be carried out on the parameters required by the experimenter, effectively maintain the consistency and repeatability of the experimental process and the chemical or physical properties of the specimens in a stable state, greatly improve the success rate of chromosome specimen preparation, and the drying fan can accelerate the air circulation at the glass slide after the solution is dropped, promote the rapid drying of the slide, improve the slide preparation efficiency, ensure the quality and stability of cell slides, thereby obtaining clearer and more stable cell images, and further improve the accuracy of testing and analysis methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of the automatic cell preparation device according to an embodiment of the present application; Figure 2 This is a schematic diagram of the structure of the incubation support according to an embodiment of the present application; Figure 3 This is a schematic structural diagram of a film drop mechanism according to an embodiment of the present application; Figure 4 A schematic diagram of the structure of the consumables mechanism of an embodiment of the present application; Figure 5 A schematic diagram of the structure of the environment adjustment mechanism of an embodiment of the present application; Figure 6 This is a schematic diagram of the structure of a humidification component according to an embodiment of the present application; Figure 7 This is a schematic diagram of the structure of the heat exchange component of an embodiment of the present application; Figure 8 This is a schematic diagram of the structure of the exhaust assembly of an embodiment of the present application.

[0017] The meanings of the reference numerals are as follows: 1. Shell; 11. Film making chamber; 2. Environmental adjustment mechanism; 21. Heat exchange assembly; 211. Heat exchange fan; 212. Heat exchange plate; 213. Heat exchange box; 22. Humidification assembly; 221. Atomization box; 222. Air pump; 223. Exhaust pipe; 23. Exhaust assembly; 231. Exhaust tube; 232. Check box; 233. Check cover; 234. Exhaust fan; 235. Valve plate; 236. Exhaust opening; 3. Consumable mechanism; 31 , consumables workbench; 32, pipette rack; 33, sample tube placement rack; 34, waste liquid tank; 35, consumables disposal tank; 4, incubation mechanism; 41, incubation bracket; 42, sample table; 43, incubation cabin; 44, storage space; 5, drop film mechanism; 51, drop film rack; 52, drop film assembly; 521, identification unit; 522, pipette gun; 6, cleaning mechanism; 61, liquid addition needle; 62, liquid discharge needle; 7; drying fan; 71, guide cover. DETAILED DESCRIPTION

[0018] For better understanding and implementation, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application.

[0019] In the description of the present application, it should be noted that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0021] See also Figure 1 and Figure 5 , the present application discloses an automatic cell preparation device. In some embodiments, the automatic cell preparation device includes a shell 1, an environmental adjustment mechanism 2, a consumable mechanism 3, an incubation mechanism 4, a film drop mechanism 5 and a drying fan 7; preferably, the shell 1 is provided with a film preparation chamber 11, and optionally, the film preparation chamber 11 is provided with a visual window, and the visual window is covered with a transparent panel, so as to close the film preparation chamber 11 to prevent the outside from interfering with the film preparation process, and the user can visually observe the film preparation process; the environmental adjustment mechanism 2 is assembled on the shell 1, and is used to adjust the environmental parameters of the film preparation chamber 11, and optionally, the environmental parameters include but are not limited to temperature and humidity, etc.; the consumable mechanism 3 is arranged in the film preparation chamber 11, and is used to accommodate film preparation consumables, and optionally, the film preparation consumables include Consumables to be used and discarded consumables, optionally, the consumables to be used include but are not limited to sample solutions or staining solutions, sample test tubes and pipette heads 522, etc., and discarded consumables include but are not limited to discarded pipette heads 522 and discarded solutions, etc.; the incubation mechanism 4 is arranged in the slide making chamber 11, for carrying glass slides; the drop film mechanism 5 is used to move between the consumable mechanism 3 and the incubation mechanism 4, when the drop film mechanism 5 moves to the consumable mechanism 3, it can take and place consumables, when the drop film mechanism 5 moves to the incubation mechanism 4, it can drip solution onto the glass slide; the drying fan 7 is connected to the drop film mechanism 5, and can move with the drop film mechanism 5, so as to accelerate the air circulation at the glass slide after dripping the solution. Preferably, the environmental parameters of the slide preparation chamber 11 are adjusted by the environmental adjustment mechanism 2 so that the slide preparation chamber 11 has the environmental parameters required for the experimental process. At the same time, the slide dropping mechanism 5 is used to take and place consumables and drop solutions, standardize experimental dosage and experimental operations, so that each batch can be carried out at the parameters required by the experimenter, effectively maintain the consistency and repeatability of the experimental process and the chemical or physical properties of the specimens in a stable state, greatly improve the success rate of chromosome specimen preparation, and the drying fan 7 can accelerate the air circulation at the glass slide after the solution is added, promote rapid drying of the slide, improve the slide preparation efficiency, ensure the quality and stability of cell slides, thereby obtaining clearer and more stable cell images, and thus improving the accuracy of testing and analysis methods.

[0022] Furthermore, it also includes electrical parts such as a main board, a driving board, and a driver, which are used to exchange information with the outside world and drive corresponding mechanisms to perform corresponding actions. Optionally, a touch plane and / or buttons can be provided to obtain user input instructions, or information can be exchanged with the outside world through communication cables and / or communication interfaces. A CPU and / or integrated circuit can be provided to process, store, send and receive data, and drive corresponding mechanisms to perform corresponding actions in combination with the steps required for the experimental process.

[0023] See also Figure 1 In some embodiments, the incubation mechanism 4 includes an incubation support 41 and a sample table 42. The incubation support 41 has an incubation chamber 43 and is arranged in the slide making chamber 11. The sample table 42 is slidably arranged in the incubation chamber 43 for carrying a glass slide, and the sample table 42 can carry the glass slide out of or accommodated in the incubation chamber 43. Optionally, the incubation support 41 is fixedly assembled in the slide making chamber 11. Optionally, a guide rail is arranged between the sample table 42 and the incubation support 41, so that the sample table 42 can move relative to the incubation support 41, so that the sample table 42 can carry the glass slide out of or accommodated in the incubation chamber 43. Optionally, one or more of the driving parts such as a mechanical arm, an electric screw rod, an electric guide rail, a motor, etc. are arranged between the sample table 42 and the incubation support 41, so that the incubation support 41 is driven by the driving part to move. Optionally, guide bars are provided on both sides of the sample stage 42, and the incubation support 41 is provided with guide rails that cooperate with the guide bars. The motor drives the guide bars to move along the guide rails, so that the guide bars drive the sample stage 42 to extend or be accommodated in the incubation chamber 43. In this embodiment, when conducting an experiment, the sample stage 42 is driven to move with the glass slide to make way for the drop film mechanism 5, avoid interference between the sample stage 42 and the drop film mechanism 5, and prevent contamination of the sample stage 42, thereby further ensuring the accuracy of the test and analysis method.

[0024] Furthermore, the lower end of the incubation support 41 is spaced apart from the inner wall of the film-making chamber 11 to form a storage space 44, and the consumable mechanism 3 is slidably disposed in the storage space 44, and the consumable mechanism 3 can extend out of or be accommodated in the storage space 44. In this way, when the film-dropping mechanism 5 takes and places consumables, the driving member drives the consumable mechanism 3 to extend out of the storage space 44, and after taking and placing, the consumable mechanism 3 retracts into the storage space 44, effectively preventing the contamination of the consumable mechanism 3 by the outside world, or the contamination of the film-making chamber 11 by the discarded consumables on the consumable mechanism 3. Optionally, the consumable mechanism 3 and the inner wall of the film making chamber 11 and / or the consumable mechanism 3 and the incubation bracket 41 are equipped with guide rails, so that the consumable mechanism 3 can be moved relative to the incubation bracket 41. Optionally, one or more driving components such as a robotic arm, an electric screw, an electric guide rail, a motor, etc. can be provided in the storage space 44, and the consumable mechanism 3 can be moved relative to the incubation bracket 41 by driving the driving component.

[0025] See also Figure 4In some embodiments, the consumables mechanism 3 includes a consumables workbench 31, a pipette rack 32, a specimen tube rack, a waste liquid tank 34, and a consumables discard tank 35. The consumables workbench 31 is slidably arranged in the storage space 44, and the pipette rack 32, the specimen tube rack, the waste liquid tank 34, and the consumables discard tank 35 are arranged on the consumables workbench 31. Optionally, a plurality of pipette guns 522 are placed on the pipette rack 32 for selection by the drop film mechanism 5. Optionally, a plurality of sample test tubes are placed on the sample tube rack 33, and the sample test tubes may store sample solutions or staining solutions, etc. Optionally, the waste liquid tank 34 is used to store discarded solutions. Optionally, the consumables discard tank 35 is used to store discarded pipette guns 522. Preferably, the consumables discarding groove 35 is located on the side of the pipette rack 32 close to the storage space 44, and the waste liquid groove 34 is located on the side of the sample tube placement rack 33 close to the storage space 44, that is, the consumables discarding groove 35 and the waste liquid groove 34 are both located on the side of the consumables workbench 31 close to the storage space 44, and the pipette rack 32 and the sample tube placement rack 33 are both located on the side of the consumables workbench 31 away from the storage space 44. In this way, when the drop film mechanism 5 needs to replace or discard consumables, the consumables workbench 31 drives the consumables discarding groove 35 and the waste liquid groove 34 to move to the desired location. The bottom of the film drop mechanism 5 is convenient for the film drop mechanism 5 to discard consumables, and then the consumables discarding tank 35 and the waste liquid tank 34 can be driven by the consumables workbench 31 to move to the storage space 44, effectively preventing the discarded consumables from splashing and contaminating the film making chamber 11, and avoiding the waste from interfering with the ongoing experiment. At the same time, it can drive the pipette rack 32 and the sample tube placement rack 33 to move to the bottom of the film drop mechanism 5, so that the film drop mechanism 5 can be equipped with a new pipette gun 522 head and / or absorb the solution, thereby ensuring that the film drop mechanism 5 can carry out the removal and placement of consumables in an orderly manner and optimizing the moving path of the film drop mechanism 5. In this embodiment, when the film drop mechanism 5 takes and places consumables, the driving member drives the consumable workbench 31 to extend out of the storage space 44, the film drop mechanism 5 discharges the discarded solution into the waste liquid tank 34, places the used pipette 522 head into the consumable discard tank 35, selects a new pipette 522 head from the pipette rack 32, and uses the new pipette 522 head to absorb the corresponding solution from the sample test tube on the sample tube placement rack 33. After taking and placing are completed, the consumable workbench 31 retracts into the storage space 44 again, effectively preventing external pollution of the consumable mechanism 3 and discarded consumables from contaminating the film making chamber 11.

[0026] See also Figure 1 , Figure 2 and Figure 3In some embodiments, the film drop mechanism 5 includes a film drop rack 51 and a film drop assembly 52, the film drop rack 51 is movably connected to the film making chamber 11, the film drop assembly 52 is movably connected to the film drop rack 51, the drying fan 7 is connected to the film drop assembly 52, and the drying fan 7 can move with the film drop assembly 52. Optionally, the drop film rack 51 is arranged in a gantry structure and can be moved along the height direction of the incubation bracket 41, so as to drive the drop film assembly 52 and the drying fan 7 to move between the consumable mechanism 3 and the incubation mechanism 4. Optionally, the drop film assembly 52 can be moved along the length direction of the drop film rack 51, so that the drop film assembly 52 can drive the drying fan 7 to move between the pipette rack 32 and the sample tube placement rack 33, or move between glass slides at different positions. In this way, when moving between glass slides at different positions, the drying fan 7 can accelerate the air circulation at the glass slide after the solution is added, thereby promoting rapid drying of the slide. Optionally, the film drop rack 51 is movably connected to the inner wall of the film making chamber 11. Optionally, the film drop rack 51 is movably connected to the incubation support 41. Specifically, the film drop rack 51 is movably connected to the incubation support 41 or the inner wall of the film making chamber 11 through a vertical driving member. A horizontal driving member is connected between the film drop assembly 52 and the film drop rack 51. Optionally, the vertical driving member and the horizontal driving member can be one or more of the driving members such as a cylinder, an electric cylinder, an electric screw, an electric guide rail, a motor, etc. Optionally, the vertical driving member and the horizontal driving member can be replaced by a multi-axis robotic arm, and the multi-axis robotic arm drives the film drop assembly 52 and the drying fan 7 to move.Preferably, the sample table 42 and the consumable workbench 31 are arranged from high to low along the height direction of the incubation bracket 41. During the experiment, the sample table 42 is accommodated in the incubation chamber 43, and the consumable workbench 31 extends out of the storage space 44, driving the consumable workbench 31 to drive the consumable discarding tank 35 and the waste liquid tank 34 to move to the bottom of the drop film mechanism 5, and the drop film rack 51 drives the drop film assembly 52 and the drying fan 7 to descend. At this time, the drying fan 7 In the closed state, the dropper assembly 52 discharges the waste solution into the waste liquid tank 34, drives the dropper assembly 52 to move horizontally above the consumables discarding tank 35, places the pipette gun 522 head to be replaced into the consumables discarding tank 35, and then the consumables workbench 31 continues to move, drives the waste liquid tank 34 and the consumables discarding tank 35 to be stored in the storage space 44, prevents the waste consumables from splashing out and contaminating the film making chamber 11, and avoids the waste from interfering with the ongoing experiment. At the same time, the pipette rack 3 2 and the sample tube placement rack 33 move to the bottom of the dripping mechanism 5, the dripping assembly 52 selects a new pipette gun 522 head from the pipette rack 32, and then moves to the top of the sample tube placement rack 33 to absorb the corresponding solution from the sample test tube, and then the dripping rack 51 drives the dripping assembly 52 and the drying fan 7 to rise and reset, and at the same time the consumables workbench 31 retracts into the storage space 44, effectively preventing the consumables mechanism 3 from being polluted by the outside world, the sample table 42 extends out of the incubation cabin 43, the dripping assembly 52 drips the solution onto the glass slide on the sample table 42, and then the drying fan 7 is started, and the drying fan 7 accelerates the air circulation at the glass slide after the solution is dripped, promotes the rapid drying of the slide, and improves the efficiency of the slide. This cycle effectively maintains the consistency and repeatability of the experimental process, greatly improves the success rate and efficiency of chromosome specimen preparation, ensures the quality and stability of cell slides, thereby obtaining clearer and more stable cell images, and thus improves the accuracy of the test and analysis methods.

[0027] Optionally, the consumable workbench 31 may be provided with a positioning component, which can accurately reflect the position of the consumable workbench 31 so that the consumable workbench 31 and the film drop mechanism 5 can better cooperate to take and place consumables. Optionally, a light-shielding sheet is provided on the consumable workbench 31, and a photoelectric sensor is provided at the position corresponding to the taking and placing of consumables in the film making chamber 11 and / or the starting point of the consumable workbench 31 (completely accommodated in the storage space 44). When the light-shielding sheet passes by the photoelectric sensor, the current position of the consumable workbench 31 can be reflected through the photoelectric sensor.

[0028] See also Figure 1 , Figure 2 and Figure 3In some embodiments, the film drop assembly 52 includes an identification unit 521 and a pipette gun 522 , the pipette gun 522 is movably connected to the film drop rack 51 , and the identification unit 521 and the drying fan 7 are both connected to the pipette gun 522 . Preferably, both the identification unit 521 and the drying fan 7 can move with the pipette gun 522; preferably, the identification unit 521 moves with the pipette gun 522, driving the identification unit 521 to identify glass slides, consumables, etc. Optionally, glass slides, consumables, etc. are provided with coded information, such as a QR code or a bar code, and the coded information is read by the identification unit 521 to record the coded information, thereby identifying the current glass slides, consumables, etc., to improve the accuracy of the experiment; in this way, during the experiment, the glass slide is identified by the identification unit 521, so that the pipette gun 522 can accurately add solution to the target glass slide, and then drive the drying fan 7 to accelerate the air circulation at the glass slide after the solution is added, thereby promoting rapid drying of the slide and improving the efficiency of the slide. Optionally, the sample table 42 carries a plurality of glass slides arranged from left to right, and the horizontal driving member drives the pipette gun 522 to drip solution onto the glass slide from left to right in sequence. The drying fan 7 is arranged on the left side of the pipette gun 522, and the identification unit 521 is arranged on the right side of the pipette gun 522. In this way, when the identification unit 521 and the drying fan 7 follow the pipette gun 522 to move from left to right, the identification unit 521 is driven to move to the top of the glass slide first, identify the coding information of the glass slide, confirm that it is correct, and then drive the pipette gun 522 to move to the top of the glass slide to drip the solution, and then drive the drying fan 7 to move to the top of the glass slide, start the drying fan 7, and accelerate the air circulation at the glass slide after the solution is dripped through the drying fan 7, promote the rapid drying of the preparation, improve the preparation efficiency, ensure the quality and stability of the cell preparation, so as to obtain a clearer and more stable cell image, thereby improving the accuracy of the test and analysis method.

[0029] Optionally, the identification unit 521 is a camera. Optionally, the pipette 522 can be automatically retracted to adjust the height, so that the pipette 522 can be extended downward to take and place consumables and adjust the distance between the pipette 522 head and the glass slide. Optionally, the pipette 522 is provided with structures such as a suction pump and a solenoid valve, which can cooperate with the pipette 522 head to complete the dripping of solution and the discharge of waste solution. Optionally, the pipette 522 can also be other types. The innovation of the present application does not lie in the specific formation method of the pipette 522, which will not be listed one by one here.

[0030] See also Figure 1 , Figure 2 and Figure 3In some embodiments, it further includes a guide cover 71 and a heating component; the air inlet of the guide cover 71 is connected to the air outlet of the drying fan 7, and the air outlet of the guide cover 71 faces the glass slide; the heating component is mounted on the sample table 42, and the glass slide is placed on the heating component. Optionally, the heating component can be a heating sheet, and the heating sheet is arranged one by one with the glass slide, and the heating sheet abuts against the bottom of the glass slide to improve the heat transfer and promote the drying of the glass slide; in this embodiment, a plurality of glass slides are arranged on the sample table 42 in sequence, and an air duct is formed between the drying fan 7 and the glass slide after the solution is added by the guide cover 71, guiding the wind blown by the drying fan 7 to blow to the glass slide after the solution is added, accurately promoting the air circulation at the glass slide after the solution is added, and the heating component abutting against the bottom of the glass slide heats the glass slide, further optimizing the drying effect and preparation efficiency of the glass slide, ensuring the quality and stability of cell preparation, and thus obtaining a clearer and more stable cell image.

[0031] See also Figure 1 , Figure 2 and Figure 3In some embodiments, a cleaning mechanism 6 is further included, and the cleaning mechanism 6 includes a liquid adding needle 61 and a liquid discharge needle 62; the liquid discharge needle 62 is mounted on the drop film rack 51, and is used to absorb the cleaning waste liquid; the liquid discharge needle 62 is connected to the drop film assembly 52, and can move with the drop film assembly 52 to release the cleaning liquid. Optionally, the cleaning liquid includes but is not limited to cleaning liquid and clean water. Optionally, a plurality of placement grooves for carrying glass slides are provided on the sample table 42, and a liquid receiving groove is provided on the surrounding side of the placement groove. When the liquid adding needle 61 cleans the glass slide, the cleaning liquid released will flow into the liquid receiving groove along the surface of the glass slide for storage. Optionally, when the drop film assembly 52 moves relative to the incubation bracket 41, the liquid adding needle 61 can move with the drop film assembly 52, so that the liquid adding needle 61 can clean the glass slide that needs to be cleaned in time, so as to ensure that the surface of the glass slide is clean and free of impurities when the drop film assembly 52 drips the solution on the glass slide. Yes, the liquid outlet needle 62 can move with the dropper rack 51. Preferably, each of the liquid receiving slots has at least one liquid suction needle, that is, each of the liquid receiving slots has at least one liquid suction needle to absorb and clean the cleaning liquid, so as to prevent the cleaning liquid splashed onto other placement slots from remaining on the placement slots or the glass slide, so as to avoid affecting subsequent processing and observation, and help ensure the accuracy and clarity of the film processing results. Optionally, the pipette gun 522 is connected to a liquid adding bracket, and a plurality of liquid adding assembly holes arranged along the length direction are provided between the liquid adding holes, and there is at least one liquid adding needle 61 in the liquid adding assembly hole. When there are multiple liquid adding needles 61 in the liquid adding assembly hole, the liquid outlet end of the liquid adding needle 61 is arranged in a divergent shape, which effectively increases the cleaning area, so that the cleaning area of ​​the liquid adding needle 61 can cover the surface of the glass slide, and fully remove any reagents or impurities that may remain on the glass slide. Optionally, the film dropper rack 51 is connected to a liquid outlet bracket, and a plurality of liquid outlet needles 62 are arranged on the liquid outlet bracket along the length direction. Optionally, the liquid aspiration bracket is provided with a plurality of buffer chambers, in which a buffer block is movably connected, the liquid aspiration needle is fixedly connected to the buffer block, and an elastic member is abutted between the upper end of the buffer block and the upper end of the buffer chamber. Preferably, when the film dropper rack 51 drives the liquid outlet needle 62 to extend into the liquid receiving tank, the liquid aspiration needle can drive the buffer block to move upward in the buffer chamber, so that the elastic member undergoes compression deformation, thereby driving the liquid aspiration needle to form elastic contact with the liquid receiving tank, thereby avoiding excessive force between the liquid aspiration needle and the liquid receiving tank, resulting in damage to the components.

[0032] See also Figure 5 and Figure 7In some embodiments, the environment adjustment mechanism 2 includes at least one heat exchange component 21; the heat exchange component 21 includes a heat exchange fan 211, a heat exchange plate 212 and a heat exchange box 213; the air outlet of the heat exchange box 213 is connected to the film-making chamber 11, and the heat exchange fan 211 is arranged between the heat exchange plate 212 and the air outlet of the heat exchange box 213. Optionally, the heat exchange plate 212 can be a heat sink or a heating plate; optionally, when the heat exchange plate 212 is a heat sink, the heat exchange plate 212 dissipates heat to the air in the heat exchange box 213, and the heat exchange fan 211 then delivers the heat-dissipated air to the film-making chamber 11, so as to reduce the temperature of the film-making chamber 11; optionally, when the heat exchange plate 212 is a heating plate, the heat exchange plate 212 heats the air in the heat exchange box 213, and the heat exchange fan 211 then delivers the heated air to the film-making chamber 11. To the film-making chamber 11, so as to increase the temperature of the film-making chamber 11. Optionally, each of the heat exchange components 21 can have a heat sink and a heating plate at the same time, and the heat sink or the heating plate is powered on and started according to the temperature of the film-making chamber 11. Optionally, the temperature of the film-making chamber 11 can be monitored by a temperature sensor arranged in the film-making chamber 11; optionally, the number of the heat exchange components 21 is two, and the heat exchange components 21 with heat sinks or heating plates are powered on and started according to the temperature of the film-making chamber 11.

[0033] See also Figure 5 and Figure 6In some embodiments, a humidifying component 22 is further included, and the humidifying component 22 includes an atomizing box 221, an air pump 222 and an air outlet pipe 223. The air inlet of the air pump 222 is connected to the film making chamber 11, the atomizing box 221 is connected to the air outlet of the air pump 222, and the air outlet pipe 223 connects the atomizing box 221 with the heat exchange box 213. Preferably, the upper end of the atomizing box 221 is also provided with a water inlet for adding water to the atomizing box 221, and the atomizing box 221 is provided with an atomizing head for atomizing the fluid in the atomizing box 221, and the atomizing head is close to the bottom of the atomizing box 221, driving the atomizing head to fully atomize the fluid stored in the atomizing box 221, avoiding the long-term residual fluid at the bottom of the atomizing box 221, effectively ensuring the hygiene of the fluid stored in the atomizing box 221, and preventing the atomized fluid from causing air pollution to the film making chamber 11. Optionally, the atomizing box 221 A water level sensor is also provided inside for monitoring the water level in the atomizing box 221; in the present embodiment, when the humidity of the film-making chamber 11 is sufficient, the air in the film-making chamber 11 can be pumped into the heat exchange box 213 through the atomizing box 221 and the air outlet pipe 223 by the vacuum pump 222, and the heat exchange plate 212 exchanges heat with the air, and then the heat exchange fan 211 transports the heat-exchanged air into the film-making chamber 11 to adjust the temperature of the film-making chamber 11. Optionally, the humidity of the film-making chamber 11 can be monitored by a humidity sensor provided in the film-making chamber 11. Optionally, when the humidity of the film-making chamber 11 is low, the atomizing head atomizes the water in the atomizing box 221, and at the same time the vacuum pump 222 transports the air in the film-making chamber 11 to the atomizing box 221, so that the air and the water mist are mixed to increase the humidity in the air. The humidified air is transported to the heat exchange box 213 through the outlet pipe 223, and then the heat exchange plate 212 exchanges heat with the air, and then the heat exchange fan 211 transports the heat-exchanged air to the film-making chamber 11, so as to achieve the adjustment of the temperature and humidity of the film-making chamber 11.

[0034] See also Figure 5 and Figure 8In some embodiments, an exhaust component 23 is further included, and the exhaust component 23 includes an exhaust pipe 231, a check box 232, a check cover 233 and an exhaust fan 234. A valve plate 235 is provided on the side of the check box 232 close to the film-making chamber 11, and the valve plate 235 is provided with an exhaust opening 236, and the exhaust opening 236 connects the film-making chamber 11 with the check box 232; the side of the check cover 233 close to the film-making chamber 11 abuts against the side of the valve plate 235 away from the film-making chamber 11, and the upper end of the check cover 233 is rotatably connected to the valve plate 235 or the check box 232; the exhaust pipe 231 is provided on the side of the check box 232 away from the film-making chamber 11, and the exhaust fan 234 connects the exhaust pipe 231 with the check box 232. Optionally, the upper end of the check cover 233 is equipped with a rotating shaft, which is rotatably connected to the rotating shaft seat, and the rotating shaft seat is fixed on the valve plate 235 or the check box 232; preferably, the exhaust fan 234 draws the air in the check box 232 to the exhaust pipe 231, and discharges it to the outside through the exhaust pipe 231. When the exhaust fan 234 sucks the check box 232, a negative pressure is formed in the check box 232. Under the action of the air pressure difference, the check cover 233 rotates around the rotating shaft, thereby opening the exhaust opening 236, driving the air in the film-making chamber 11 to flow into the check box 232, so as to exhaust the film-making chamber 11. When the exhaust fan 234 stops working, the air pressure in the check box 232 returns to normal, and the check cover 233 is closed under the influence of its own gravity. , rotates in the opposite direction around the rotating shaft so that the side of the check cover 233 close to the film-making chamber 11 abuts against the side of the valve plate 235 away from the film-making chamber 11, thereby closing the exhaust opening 236 and preventing external gas from passing through the exhaust opening 236 to prevent the film-making chamber 11 from being polluted by external air; optionally, a silicone pad is provided between the check cover 233 and the valve plate 235 to improve the sealing effect between the check cover 233 and the valve plate 235, optionally, the side of the valve plate 235 away from the film-making chamber 11 extends in the direction away from the film-making chamber 11 to form an abutting slope, and the side of the check cover 233 close to the film-making chamber 11 abuts against the abutting slope to ensure that the check cover 233 can abut against the valve plate 235 to close the exhaust opening 236.

[0035] See also Figure 1-Figure 8In some embodiments, the vacuum pump 222 pumps the air in the preparation chamber 11 into the atomizing box 221, and the atomizing head in the atomizing box 221 atomizes the water in the atomizing box 221, drives the atomized water to mix with the air, increases the humidity in the air, and then transports the humidified air to the heat exchange box 213 through the outlet pipe 223, and the heat exchange plate 212 exchanges heat with the humidified air to adjust the temperature of the humidified air, and then the heat exchange fan 211 transports the heat-exchanged air to the preparation chamber 11, so as to adjust the temperature and humidity of the preparation chamber 11 in an internal circulation manner, effectively ensure the efficiency of the internal circulation of the air, and optimize the effect of adjusting the temperature and humidity of the preparation chamber 11, so that the preparation chamber 11 has the environmental parameters required for the experimental process; optionally, the air in the check box 232 can be pumped to the preparation chamber 11 by the exhaust fan 234. The exhaust fan 234 is turned off, and the air pressure in the check box 232 returns to normal. The check cover 233 rotates in the opposite direction under the influence of its own gravity, so that the side of the check cover 233 close to the making chamber 11 abuts against the side of the valve plate 235 away from the making chamber 11, thereby closing the exhaust opening 236 and preventing the external airflow from flowing back into the making chamber 11 from the exhaust opening 236, thereby preventing the making chamber 11 from being polluted by the external air.In the cleaning step, the slide dropper rack 51 drives the liquid adding needle 61 and the liquid outlet needle 62 to move downward, so that the liquid adding needle 61 is close to the glass slide, and the liquid outlet needle 62 extends into the liquid receiving tank. The cleaning liquid released by the liquid adding needle 61 cleans the glass slide, and then the cleaning liquid is sucked away and cleaned by the liquid outlet needle 62 to ensure that the surface of the glass slide is clean and free of impurities. Furthermore, the drying fan 7 can also accelerate the air circulation at the cleaned glass slide, accelerate the drying of the glass slide, and improve the production efficiency. Optionally, when the drying fan 7 accelerates the air flow, the glass slide is heated by the heating component abutting against the bottom of the glass slide, which can further optimize the drying effect and production efficiency of the glass slide. In the step of adding solution, the sample table 42 is accommodated in the incubation chamber 43, and the consumable workbench 31 extends out of the storage space 44, driving the consumable workbench 31 to drive the consumable discarding tank 35 and the waste liquid tank 34 to move to the bottom of the drop film mechanism 5, and the drop film rack 51 drives the pipette gun 522 and the drying fan 7 to descend. At this time, the drying fan 7 is in a closed state, and the pipette gun 522 discharges the waste solution into the waste liquid tank 34, driving the pipette gun 522 to move horizontally to the top of the consumable discarding tank 35, and placing the replaced pipette gun 522 head in the consumable discarding tank 35, and then the consumable workbench 31 continues to move, The waste liquid tank 34 and the consumables discard tank 35 are driven to be stored in the storage space 44 to prevent the waste consumables from splashing out and contaminating the film making chamber 11, and to avoid the waste from interfering with the ongoing experiment. At the same time, the pipette rack 32 and the sample tube placement rack 33 are moved to the bottom of the drop film mechanism 5, and the pipette gun 522 selects a new pipette gun 522 head from the pipette rack 32, and the pipette gun 522 is moved to the top of the sample tube placement rack 33, and the new pipette gun 522 head is used to absorb the corresponding solution from the sample test tube, and then the drop film rack 51 drives the pipette gun 522 and the drying fan 7 to rise and reset, and at the same time the consumables workbench 31 is retracted. The storage space 44 effectively prevents external pollution of the consumables mechanism 3. The sample table 42 extends out of the incubation chamber 43, and then moves to drip the solution onto the glass slide on the sample table 42, and then drives the drying fan 7 to move above the glass slide, and starts the drying fan 7. The drying fan 7 accelerates the air circulation at the glass slide after dripping the solution, promotes the rapid drying of the slide, and improves the production efficiency. Such a cycle effectively maintains the consistency and repeatability of the experimental process, greatly improves the success rate and production efficiency of chromosome specimen preparation, ensures the quality and stability of cell preparation, thereby obtaining clearer and more stable cell images, and thus improves the accuracy of testing and analysis methods.

[0036] The technical means disclosed in the present application are not limited to the technical means disclosed in the above-mentioned implementation methods, but also include technical solutions composed of any combination of the above technical features. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of this application, and these improvements and modifications are also regarded as the protection scope of this application.

Claims

1. An automatic cell preparation device, characterized in that: include: The housing (1) is provided with a film-making chamber (11); An environmental adjustment mechanism (2), the environmental adjustment mechanism (2) being mounted on the housing (1) and used for adjusting environmental parameters of the film-making chamber (11); A consumables mechanism (3), arranged in the film making chamber (11), and used for accommodating film making consumables; An incubation mechanism (4) is arranged in the slide preparation chamber (11) and is used to carry the slide; A film dropping mechanism (5) is used to move between the consumables mechanism (3) and the incubation mechanism (4); when the film dropping mechanism (5) moves to the consumables mechanism (3), it can take in and put in consumables; when the film dropping mechanism (5) moves to the incubation mechanism (4), it can drop a solution onto the glass slide; A drying fan (7), the drying fan (7) is connected to the slide dropping mechanism (5), can move along with the slide dropping mechanism (5), and is used to accelerate the air circulation at the slide after the solution is dropped.

2. The automatic cell preparation device according to claim 1, characterized in that: The film dropping mechanism (5) comprises a film dropping frame (51) and a film dropping assembly (52), wherein the film dropping frame (51) is movably connected to the film making chamber (11), and the film dropping assembly (52) is movably connected to the film dropping frame (51), and the drying fan (7) is connected to the film dropping assembly (52), and the drying fan (7) can move along with the film dropping assembly (52).

3. The automatic cell preparation device according to claim 2, characterized in that: The film drop assembly (52) comprises an identification unit (521) and a pipette gun (522), wherein the pipette gun (522) is movably connected to the film drop rack (51), and the identification unit (521) and the drying fan (7) are both connected to the pipette gun (522).

4. The automatic cell preparation device according to claim 1, characterized in that: Also includes a guide cover (71) and a heating assembly; The air inlet of the guide cover (71) is connected to the air outlet of the drying fan (7), and the air outlet of the guide cover (71) faces the glass slide; The heating component is assembled on the incubation mechanism (4), and the glass slide is placed on the heating component.

5. The automatic cell preparation device according to claim 2, characterized in that: It also includes a cleaning mechanism (6), wherein the cleaning mechanism (6) includes a liquid adding needle (61) and a liquid discharging needle (62); The liquid outlet needle (62) is mounted on the film dropper frame (51) and is used to absorb the cleaning waste liquid; The liquid outlet needle (62) is connected to the drop sheet assembly (52) and can move along with the drop sheet assembly (52) to release the cleaning liquid.

6. The automatic cell preparation device according to claim 1, characterized in that: The incubation mechanism (4) comprises an incubation support (41) and a sample table (42); The incubation support (41) is arranged in the film-making chamber (11), the incubation support (41) has an incubation chamber (43) inside, and the lower end of the incubation support (41) is spaced apart from the inner wall of the film-making chamber (11) to form a storage space (44), the consumable mechanism (3) is slidably arranged in the storage space (44), and the consumable mechanism (3) can extend out of or be accommodated in the storage space (44); The sample stage (42) is slidably mounted on the incubation chamber (43) and is used to carry a glass slide. The sample stage (42) can carry the glass slide out of or be accommodated in the incubation chamber (43).

7. The automatic cell preparation device according to claim 6, characterized in that: The consumables mechanism (3) comprises a consumables workbench (31), a pipette rack (32), a sample tube placement rack (33), a waste liquid tank (34) and a consumables discard tank (35); the consumables workbench (31) is slidably arranged in the storage space (44); the pipette rack (32), the sample tube placement rack (33), the waste liquid tank (34) and the consumables discard tank (35) are arranged on the consumables workbench (31).

8. The automatic cell preparation device according to claim 1, characterized in that: The environment adjustment mechanism (2) comprises at least one heat exchange component (21); The heat exchange component (21) comprises a heat exchange fan (211), a heat exchange plate (212) and a heat exchange box (213); an air outlet of the heat exchange box (213) is connected to the film making chamber (11); and the heat exchange fan (211) is arranged between the heat exchange plate (212) and the air outlet of the heat exchange box (213).

9. The automatic cell preparation device according to claim 8, characterized in that: The film further comprises a humidifying component (22), wherein the humidifying component (22) comprises an atomizing box (221), an air pump (222) and an air outlet pipe (223), wherein the air inlet of the air pump (222) is connected to the film making chamber (11), the atomizing box (221) is connected to the air outlet of the air pump (222), and the air outlet pipe (223) connects the atomizing box (221) to the heat exchange box (213).

10. The automatic cell preparation device according to claim 1, characterized in that: The invention also comprises an exhaust assembly (23), wherein the exhaust assembly (23) comprises an exhaust cylinder (231), a check box (232), a check cover (233) and an exhaust fan (234). A valve plate (235) is provided on one side of the check box (232) close to the film-making chamber (11), and the valve plate (235) is provided with an exhaust opening (236), and the exhaust opening (236) connects the film-making chamber (11) with the check box (232); The side of the check cover (233) close to the film-making chamber (11) abuts against the side of the valve plate (235) away from the film-making chamber (11), and the upper end of the check cover (233) is rotatably connected to the valve plate (235) or the check box (232); The exhaust pipe (231) is arranged on a side of the check box (232) away from the film making chamber (11), and the exhaust fan (234) connects the exhaust pipe (231) and the check box (232).

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