Mouse hypoxia experiment device

Abstract

The invention discloses a mouse hypoxia experiment device in the technical field of medical experiment equipment, and the mouse hypoxia experiment device comprises a base, the base is fixedly provided with a box body, the base is further rotatably connected with a rotating disc for sealing the bottom of the box body, the box body comprises a feeding area and an object placing area, the feeding area is provided with a feeding opening, the object placing area is provided with an object placing opening, and the object placing area is provided with an opening. The rotating disc is provided with an object containing groove which is alternately communicated with the feeding opening and the object placing opening; an inner groove is formed in the rotating disc, an air bag is fixedly arranged in the inner groove, the air bag comprises an air inlet end and an air outlet end, the inner groove is further slidably connected with an extrusion plate used for extruding the air bag, and the extrusion plate is fixedly connected with a sliding rod extending out of the rotating disc; the base is fixedly provided with a ring body located below the rotating disc, the top of the ring body is fixedly provided with an arc-shaped extrusion part, and the extrusion part comprises a horizontal tangent plane and inclined planes connected with the two sides of the tangent plane. According to the invention, the accuracy of experimental results can be improved.

Description

Technical Field

[0001] The invention relates to the technical field of medical experimental equipment, and in particular to a mouse hypoxia experimental device. Background Art

[0002] Mouse hypoxia experiment is a commonly used biological and medical research method, which is used to replicate the hypoxia animal model and observe the body's response and changes during the hypoxia process. Hypoxia refers to a pathological process in which tissue metabolism, function and even morphological structure undergo abnormal changes when the tissue is not supplied with enough oxygen or cannot fully utilize oxygen. Depending on the cause of hypoxia, hypoxia can be divided into four types: hypotonic hypoxia, hemorrhagic hypoxia, circulatory hypoxia and tissue hypoxia. In the hypoxic hypoxia experiment, a mouse is placed in a sealed container containing soda lime to absorb the carbon dioxide in the bottle, thereby simulating a hypotonic hypoxic environment. The general condition of the mouse, the color of the lips and tail skin, the number and depth of respiration, etc. are observed and recorded.

[0003] As shown in the prior art CN216796089U, a hypoxia experimental device for mouse research, mice survive longer in a relatively low oxygen concentration environment during hypoxia experiments. If they are not supplied with food, the experiment will be greatly affected. Supplying food will inevitably cause a temporary change in the relatively low oxygen concentration environment, affecting the accuracy of the experiment. For this reason, the above-mentioned prior art delivers the required food into the interior of the box through a sealed feeding component. Specifically, the food is placed into the storage hopper, and the sealing cover is closed. The screw rod is driven to rotate by turning the rocker, so that the food can be transmitted to the interior of the box through the cylinder. However, the above method still has the disadvantage of bringing outside air into the box. For example, the screw rod has a certain depth of thread, and the air in the space between the threads, for example, when food is placed into the storage hopper, the outside air enters the storage hopper, thereby affecting the accuracy of the experimental results. Summary of the invention

[0004] The present invention is intended to provide a mouse hypoxia experimental device, so as to provide an experimental device which can reduce the accidental entry of air and improve the experimental accuracy.

[0005] In order to solve the above problems, the present invention provides the following technical solutions:

[0006] A mouse hypoxia experimental device comprises a base, a box is fixedly provided on the base, the base is also rotatably connected to a rotating disk that closes the bottom of the box, the box comprises a feeding area and a placing area, the feeding area is provided with a feeding port, the placing area is provided with a placing port, the rotating disk is provided with a loading groove that is alternately connected with the feeding port and the placing port; an inner groove is provided inside the rotating disk, an air bag is fixedly provided in the inner groove, the air bag comprises an air inlet end and an air outlet end, the air inlet end extends into the loading groove, the air outlet end extends out of the rotating disk, the air inlet end is provided with a first A one-way valve, the air outlet end is provided with a second one-way valve that is conductive to the outside of the airbag, the inner groove is also slidably connected with an extrusion plate for extruding the airbag, and the extrusion plate is fixedly connected with a sliding rod that extends out of the rotating disk; the base is fixedly provided with a ring body located below the rotating disk, and the top of the ring body is fixedly provided with an arc-shaped extrusion portion, and the extrusion portion includes a horizontal section and an inclined surface connected to both sides of the section, the sliding rod and the inclined surface and the section are alternately abutted, and the two sides of the section and the two sides of the discharge port have an angle in the circumferential direction; the base is also fixedly provided with a motor that drives the rotating disk to rotate.

[0007] Working principle and beneficial effects of the present invention:

[0008] In this scheme, mice are placed in the feeding area for a hypoxia experiment. The loading trough and the feeding port are staggered, so that the rotating disk closes the feeding port and the feeding area is in a closed state. The rotating disk is driven by a motor to rotate. When the sliding rod passes through a side slope, the slope squeezes the sliding rod upward, and the sliding rod squeezes the airbag through the squeezing plate to compress the airbag. The gas in the airbag is discharged outward through the second one-way valve and the air outlet. When the squeezing plate rotates to contact the cut surface, since the cut surface is horizontal, the cut surface keeps squeezing the sliding rod, so that the airbag remains in a compressed state. Then the loading trough on the rotating disk rotates to connect with the discharge port, and food or medicine is placed in the loading trough through the discharge port. The motor continues to drive the rotating disk to rotate. Since the two sides of the cut surface and the two sides of the discharge port have an angle in the circumferential direction, the airbag is always kept in a compressed state during the staggered process of the loading trough and the discharge port. Then the loading trough is connected to the discharge port. The groove rotates to abut against the bottom of the box body, and the bottom of the box body seals the loading groove. When the sliding rod passes from the cut surface to the inclined surface on the other side, the sliding rod descends from the inclined surface on the other side, thereby providing space for the air bag to swell. The setting of the conduction direction of the first one-way valve and the second one-way valve allows the air in the loading groove to be sucked into the air bag through the air inlet end when the air bag is swollen, thereby minimizing the air in the loading groove. When the loading groove rotates to be connected with the feeding port, since the air in the loading groove is almost sucked out by the air bag, it is avoided that the outside air is brought into the feeding area. The mouse can pick up the medicine or food in the loading groove through the feeding port to eat, and the rotating disk always keeps the feeding port closed during the whole process.

[0009] This solution uses the inclined surfaces and cut surfaces on both sides to rotate with the rotating disk to remove the air in the trough, thereby preventing the air in the trough from being brought into the feeding area, and keeping the feeding port closed during the entire process, thereby ensuring that the feeding area is in a relatively closed state, avoiding oxygen supplementation in the feeding area, and improving the accuracy of the experiment. Secondly, in the mouse hypoxia experiment, the food in the trough is constantly replaced to ensure that the mice always eat fresh and clean food to eliminate the impact of food on the mice. In addition, the prior art is to feed by extruding a spiral rod. First of all, this method must ensure that the food being fed is liquid food, and solid food will clog the spiral rod. If the spiral rod cannot crush the solid food, it will cause jamming. Solid food has a longer shelf life than liquid food. Therefore, by adopting this solution, the fixed food can be directly placed in the loading trough for direct feeding, thereby providing complete fixed food and reducing the number of times the food is changed, thereby ensuring that the feeding area is in a closed and static state as much as possible. In addition, some solid medicines will be added in the mouse hypoxia experiment to assist the experiment, and the prior art adopts a spiral rod method, which will also crush the solid medicines, which will change the medicinal properties of the solid medicines. Therefore, it is not advisable to adopt the prior art, and the present application can directly put the solid medicines into the loading trough for direct feeding, thereby not changing the medicinal properties of the solid medicines. In summary, the present application can improve the accuracy of the experimental results.

[0010] Optimally, the sliding rod and the rotating disk are vertically slidably connected. This optimization scheme can provide as much space as possible for the air bag to deform, thereby improving the air bag's ability to wash out the air in the loading tank.

[0011] Optimally, the number of the inner grooves is multiple, and medicines or multiple foods can be placed in the multiple inner grooves to ensure the growth needs of mice.

[0012] Preferably, the motor is a servo motor, which has the functions of precise steering and self-locking of the driving end.

[0013] Preferably, the number of the squeezing parts is multiple and they are evenly spaced along the circumferential direction of the ring body. When the rotating disk rotates, the multiple squeezing parts are used to repeatedly squeeze and release the airbag, so that the air in the loading tank is repeatedly sucked out during the swelling and compression of the airbag.

[0014] Optimally, a limit spring is fixedly arranged between the extrusion plate and the inner groove, and the airbag is fixedly arranged between the extrusion plate and the inner groove. When the extrusion plate squeezes the airbag for compression, the limit spring is compressed, and when the sliding rod on the extrusion plate is in an active state, the compressed spring quickly recovers, thereby pulling the airbag to quickly recover through the extrusion plate, thereby quickly improving the instantaneous suction force at the air inlet end.

[0015] Optimally, the loading trough is detachably provided with a loading box, through which food and medicine can be conveniently loaded.

[0016] Optimally, the feeding area is provided with a feeding box. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is an internal structure diagram of a mouse hypoxia experimental device of the present invention;

[0018] Figure 2 for Figure 1 A schematic diagram of the structure when the middle loading slot and the loading port are aligned;

[0019] Figure 3 for Figure 1 Top view of the middle ring body;

[0020] Figure 4 for Figure 1 Top view of the rotating disk. DETAILED DESCRIPTION

[0021] The following is further described in detail through specific implementation methods:

[0022] The figure marks in the drawings of the specification include: base 1, placement port 2, box body 3, sealing cover 4, feeding port 5, loading trough 6, air inlet end 7, air bag 8, extrusion plate 9, extrusion rod 10, motor 11, ring body 12, section 13, rotating disk 14, air outlet end 15, first inclined surface 16, feeding area 17, placement area 18, second inclined surface 19.

[0023] In the following statements, directional words such as "left", "right", "up", and "down" are all based on the directions shown in the diagram. In practice, if the corresponding structures are changed in the same direction based on the directions while keeping the relative positions unchanged, it will not affect the implementation of the plan.

[0024] Embodiment: A mouse hypoxia experimental device, such as Figure 1 and Figure 4 As shown, the base 1 includes a box body 3 fixedly mounted on the base 1. The base 1 is also rotatably connected to a rotating disk 14 that seals the bottom of the box body 3. The box body 3 includes a feeding area 17 and a storage area 18. The feeding area 17 is equipped with a sealing cover 4.

[0025] The feeding area 17 is provided with a feeding port 5, the placing area 18 is provided with a placing port 2, and the rotating disk 14 is provided with a loading trough 6 alternately connected with the feeding port 5 and the placing port 2;

[0026] An inner groove is provided inside the rotating disk 14, and an air bag 8 is fixedly provided in the inner groove. The air bag 8 includes an air inlet end 7 and an air outlet end 15. The air inlet end 7 extends into the loading groove 6, and the air outlet end 15 extends out of the rotating disk 14. The air inlet end 7 is provided with a first one-way valve that is connected toward the air bag 8, and the air outlet end 15 is provided with a second one-way valve that is connected toward the outside of the air bag 8. The inner groove is also vertically slidably connected to an extrusion plate 9 for extruding the air bag 8, and the extrusion plate 9 is fixedly connected to a sliding rod that extends out of the rotating disk 14.

[0027] like Figure 3 As shown, the base 1 is fixedly provided with a ring body 12 located below the rotating disk 14, and the top of the ring body 12 is fixedly provided with an arc-shaped extrusion portion, which includes a horizontal section 13 and inclined surfaces connected to both sides of the section 13, and the inclined surfaces are respectively a first inclined surface 16 and a second inclined surface 19, and the sliding rod is alternately abutted against the first inclined surface 16, the second inclined surface 19, and the section 13, and the two sides of the section 13 and the two sides of the discharge port 2 have an angle in the circumferential direction; the base 1 is also fixedly provided with a motor 11 for driving the rotating disk 14 to rotate.

[0028] In this scheme, mice are placed in the feeding area 17, and the sealing cover 4 is installed to carry out the hypoxia experiment. Figure 1As shown, at this time, the loading trough 6 and the feeding port 5 are opposite, and the mice can pick up the food in the loading trough 6. After a period of time, the feces and the like contaminate the food, and the food needs to be replaced. The rotating disk 14 is driven by the motor 11 to rotate counterclockwise. When the sliding rod passes through the first inclined surface 16, the first inclined surface 16 presses the sliding rod upward, and the sliding rod compresses the airbag 8 through the squeezing plate 9. The gas in the airbag 8 is discharged outward through the second one-way valve and the air outlet 15. When the squeezing plate 9 rotates to contact the cut surface 13, since the cut surface 13 is horizontal, the cut surface 13 keeps squeezing the sliding rod, so that the airbag 8 remains in a compressed state. Then the loading trough 6 on the rotating disk 14 rotates to communicate with the discharge port 2, and fresh food or medicine is placed in the loading trough 6 through the discharge port 2. The motor 11 continues to drive the counterclockwise rotation. Since the two sides of the cut surface 13 and the two sides of the discharge port 2 have an angle in the circumferential direction, the airbag 8 is always kept in a compressed state during the staggered process of the loading trough 6 and the discharge port 2. Then, the loading trough 6 rotates to abut against the bottom of the box body 3, and the bottom of the box body 3 seals the loading trough 6. When the sliding rod passes from the cut surface 13 to the second inclined surface 19, the sliding rod descends from the second inclined surface 19, thereby providing space for the airbag 8 to swell, and the elastic airbag 8 swells. The setting of the conduction direction of the first one-way valve and the second one-way valve allows the air in the loading trough 6 to be sucked into the airbag 8 through the air inlet end 7 when the airbag 8 swells, thereby minimizing the air in the loading trough 6. When the loading trough 6 rotates to be connected to the feeding port 5, since the air in the loading trough 6 is almost sucked out by the airbag 8, it is avoided that the outside air is brought into the feeding area 17. The mouse can pick up the medicine or food in the loading trough 6 through the feeding port 5 to eat, and the rotating disk 14 always keeps the feeding port 5 closed during the whole process.

[0029] In this solution, the first inclined surface 16, the second inclined surface 19 and the cut surface 13 cooperate with the rotating disk 14 to rotate, so as to remove the air in the loading trough 6, thereby preventing the air in the loading trough 6 from being brought into the feeding area 17, and keeping the feeding port 5 closed during the whole process, thereby ensuring that the feeding area 17 is in a relatively closed state, avoiding oxygen supplementation in the feeding area 17, and improving the accuracy of the experiment. Secondly, in the mouse hypoxia experiment, by constantly changing the food in the loading trough 6, it is ensured that the mice always eat fresh and clean food to eliminate the influence of food on the mice. In addition, the prior art is to feed by screw extrusion. First of all, this method must ensure that the food being fed is liquid food, and solid food will clog the screw rod. When the screw rod cannot crush the solid food, it will cause jamming. Compared with liquid food, solid food has a longer shelf life. Therefore, by adopting this solution, the fixed food can be directly placed in the loading trough 6 for direct feeding, thereby providing complete fixed food and reducing the number of times the food is changed, thereby ensuring that the feeding area 17 is in a closed and static state as much as possible. In addition, some solid medicines will be added in the mouse hypoxia experiment to assist the experiment. The prior art uses a screw rod, which will also crush the solid medicines, which will change the medicinal properties of the solid medicines. Therefore, it is not advisable to adopt the prior art, and the present application can directly put the solid medicines into the loading trough 6 for direct feeding, thereby not changing the medicinal properties of the solid medicines. In summary, the present application can improve the accuracy of the experimental results.

[0030] Preferably, in order to improve the sealing effect, a layer of rubber pad is provided on the rotating disk 14, and the sealing performance is improved by squeezing the rubber pad to produce deformation.

[0031] In addition, a limit spring is fixedly provided between the squeezing plate 9 and the inner groove, and the airbag 8 is fixedly provided between the squeezing plate 9 and the inner groove. When the squeezing plate 9 squeezes the airbag 8 to compress it, the limit spring is compressed, and when the sliding rod on the squeezing plate 9 is in an active state, the compressed spring quickly recovers, thereby pulling the airbag 8 to quickly recover through the squeezing plate 9, thereby quickly improving the instantaneous suction force of the air inlet end 7.

Claims

1. A mouse hypoxia experimental device, comprising a base, wherein the base is fixed with a box, characterized in that: The base is also rotatably connected to a rotating disk at the bottom of a closed box body, the box body includes a feeding area and a placing area, the feeding area is provided with a feeding port, the placing area is provided with a placing port, the rotating disk is provided with a loading groove alternately connected with the feeding port and the placing port; an inner groove is provided inside the rotating disk, an air bag is fixedly provided in the inner groove, the air bag includes an air inlet end and an air outlet end, the air inlet end extends into the loading groove, the air outlet end extends out of the rotating disk, the air inlet end is provided with a first one-way valve conducting in the direction of the air bag, and the air outlet end is provided with a first one-way valve conducting in the direction of the air bag, and the air outlet end is provided with a first one-way valve conducting in the direction of the air bag. A second one-way valve is conducted outside the airbag, and the inner groove is also slidably connected with an extrusion plate for extruding the airbag, and the extrusion plate is fixedly connected with a sliding rod extending out of the rotating disk; the base is fixedly provided with a ring body located below the rotating disk, and the top of the ring body is fixedly provided with an arc-shaped extrusion portion, and the extrusion portion includes a horizontal section and inclined surfaces connected to both sides of the section, the sliding rod and the inclined surfaces and the section are alternately abutted, and the two sides of the section and the two sides of the discharge port have an angle in the circumferential direction; the base is also fixedly provided with a motor for driving the rotating disk to rotate.

2. The mouse hypoxia experimental device according to claim 1, characterized in that: The sliding rod and the rotating disk are vertically slidably connected.

3. The mouse hypoxia experimental device according to claim 2, characterized in that: The number of the inner grooves is multiple.

4. The mouse hypoxia experimental device according to claim 3, characterized in that: The motor is specifically a servo motor.

5. The mouse hypoxia experimental device according to any one of claims 1 to 4, characterized in that: There are multiple extrusion parts, which are evenly spaced along the circumferential direction of the ring body.

6. The mouse hypoxia experimental device according to claim 5, characterized in that: A limit spring is fixedly arranged between the extrusion plate and the inner groove.

7. The mouse hypoxia experimental device according to claim 6, characterized in that: The charging trough is detachably provided with a charging box.

8. The mouse hypoxia experimental device according to claim 7, characterized in that: The feeding area is provided with a charging box.

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