Fresh water ecdysis regulator for penaeus vannamei and preparation method and application of fresh water ecdysis regulator

Abstract

The invention discloses a fresh water ecdysis regulator for penaeus vannamei as well as a preparation method and application of the fresh water ecdysis regulator. The fresh water ecdysis regulator for the penaeus vannamei is prepared from the following raw materials in parts by weight: 2-5 parts of calcium lactate, 0.01-0.02 part of vitamin C, 0.01-0.02 part of vitamin E, 4-6 parts of magnesium sulfate, 0.2-0.4 part of arginine, 5-10 parts of cyanotis arachnoidea, 6-8 parts of hawthorn, 5-10 parts of yeast powder, 10-20 parts of brown sugar, 5-8 parts of gynostemma pentaphyllum and 40-60 parts of rare earth. The molting regulator for freshwater aquaculture of the penaeus vannamei can promote molting of the penaeus vannamei by selecting the raw materials and dosage, solves the problem that the penaeus vannamei grows slowly in a freshwater aquaculture environment, is beneficial to reducing the morbidity of the penaeus vannamei and improving the survival rate and the aquaculture yield of the penaeus vannamei, and has a wide application prospect. The method has important scientific significance and practical application value for freshwater aquaculture of the penaeus vannamei boone.

Description

Technical Field

[0001] The invention relates to the technical field of shrimp breeding, and in particular to a freshwater molting regulator for whiteleg shrimp (Penaeus vannamei), a preparation method and application thereof. Background Art

[0002] The white shrimp is delicious, nutritious, and can be used to make a variety of delicacies. It is rich in protein, magnesium, calcium, phosphorus, potassium, iodine, and vitamin A. It is a longevity food, beauty food, and a good product for nourishing qi and yang. Due to its strong disease resistance and fast growth rate, white shrimp has been widely cultivated in coastal areas. At the same time, with the gradual maturity of desalination and breeding technology, artificial freshwater aquaculture in inland areas is also increasing. Therefore, it is a very promising shrimp farming variety.

[0003] The reproduction of whiteleg shrimp in fresh water has not been successful so far. The supply of shrimp fry is provided by coastal high-salinity seawater breeding grounds. Since the shrimp fry enter the fresh water directly from the high-salinity seawater, the growth of the shrimp will be affected, and the breeding effect cannot be achieved.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide a freshwater molting regulator for Penaeus vannamei, a preparation method and an application thereof. The present invention can promote the molting of the shrimp by adding a molting regulator suitable for the growth of Penaeus vannamei during the breeding process, solves the problem of slow growth of Penaeus vannamei in a freshwater breeding environment, is beneficial to reducing the incidence of Penaeus vannamei, and improving the survival rate and breeding yield of the shrimp. The present invention has important scientific significance and practical application value for the freshwater breeding of Penaeus vannamei.

[0006] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0007] The first aspect of the present invention provides a freshwater molting regulator for Penaeus vannamei, wherein the freshwater molting regulator for Penaeus vannamei is composed of the following raw materials in parts by weight:

[0008] 2-5 parts of calcium lactate, 0.01-0.02 parts of vitamin C, 0.01-0.02 parts of vitamin E, 4-6 parts of magnesium sulfate, 0.2-0.4 parts of arginine, 5-10 parts of dew grass, 6-8 parts of hawthorn, 5-10 parts of yeast powder, 10-20 parts of brown sugar, 5-8 parts of Gynostemma pentaphyllum and 40-60 parts of rare earth.

[0009] Preferably, the white shrimp freshwater molting regulator is composed of the following raw materials in parts by weight:

[0010] 3 parts of calcium lactate, 0.01 parts of vitamin C, 0.02 parts of vitamin E, 5 parts of magnesium sulfate, 0.3 parts of arginine, 8 parts of dew grass, 7 parts of hawthorn, 7 parts of yeast powder, 15 parts of brown sugar, 6 parts of gynostemma pentaphyllum and 50 parts of rare earth.

[0011] Preferably, the rare earth is yttrium oxide.

[0012] The second aspect of the present invention provides a method for preparing the above-mentioned white shrimp freshwater molting regulator, the preparation method comprising the following steps:

[0013] Drying and crushing the dew grass, Gynostemma pentaphyllum and Crataegus pinnatifida respectively;

[0014] Weigh each raw material according to weight, and mix the raw materials evenly to obtain the white shrimp freshwater molting regulator.

[0015] A third aspect of the present invention provides a use of the above-mentioned Penaeus vannamei freshwater molting regulator in freshwater aquaculture of Penaeus vannamei.

[0016] A fourth aspect of the present invention provides a freshwater culture method for Penaeus vannamei, the freshwater culture method for Penaeus vannamei comprising the following steps:

[0017] The whiteleg shrimp fry are put into the water body for breeding, and mixed feed is fed during the breeding process, wherein the mixed feed consists of the whiteleg shrimp freshwater molting regulator and special feed for shrimp.

[0018] Preferably, during the breeding process, the water pH is controlled at 7.8-9.0, the dissolved oxygen is above 5 mg / L, and the temperature is controlled at 25-28°C.

[0019] Preferably, the shrimp-specific feed is fed 3 to 5 times a day during the breeding process, and the feeding amount each time is 80 to 120 g per 10,000 shrimps.

[0020] Preferably, after the whiteleg shrimp fry are released, the mixed feed is fed once every 3 to 4 days, and the feeding amount is 80 to 120 g / 10,000 shrimps; after 20 to 25 days of cultivation, the mixed feed is fed once every 6 to 8 days, and the feeding amount is 80 to 120 g / 10,000 shrimps.

[0021] Preferably, the mass ratio of the freshwater molting regulator for whiteleg shrimp to the special feed for shrimp in the mixed feed is 1:(90-110).

[0022] Compared with the prior art, the beneficial effects of the present invention include at least:

[0023] The whiteleg shrimp freshwater aquaculture molting regulator provided by the invention can promote the molting of the whiteleg shrimp by selecting various raw materials and dosages, solves the problem of slow growth of the whiteleg shrimp in a freshwater aquaculture environment, is beneficial to reducing the incidence of the whiteleg shrimp, and improves the survival rate and aquaculture yield of the shrimp, and has important scientific significance and practical application value for the freshwater aquaculture of the whiteleg shrimp.

[0024] The breeding method of the present invention is simple to operate, easy to implement, and has the characteristics of fast growth and high survival rate of shrimp breeding. It provides a low-cost method for promoting the growth of freshwater breeding of white shrimp in rice fields, ponds, reservoirs, lakes and other freshwater areas, solves the problem of slow growth of white shrimp in freshwater breeding environments, and provides technical support for freshwater breeding of white shrimp. DETAILED DESCRIPTION

[0025] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the embodiments. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore only used as examples, and cannot be used to limit the protection scope of the present invention.

[0026] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the invention belongs.

[0027] The embodiment of the present invention provides a freshwater molting regulator for Penaeus vannamei. The freshwater molting regulator for Penaeus vannamei is composed of the following raw materials in parts by weight.

[0028] 2-5 parts of calcium lactate, 0.01-0.02 parts of vitamin C, 0.01-0.02 parts of vitamin E, 4-6 parts of magnesium sulfate, 0.2-0.4 parts of arginine, 5-10 parts of dew grass, 6-8 parts of hawthorn, 5-10 parts of yeast powder, 10-20 parts of brown sugar, 5-8 parts of Gynostemma pentaphyllum and 40-60 parts of rare earth.

[0029] The whiteleg shrimp freshwater aquaculture molting regulator provided by the invention can promote the molting of the whiteleg shrimp by selecting various raw materials and dosages, solves the problem of slow growth of the whiteleg shrimp in a freshwater aquaculture environment, is beneficial to reducing the incidence of the whiteleg shrimp, and improves the survival rate and aquaculture yield of the shrimp, and has important scientific significance and practical application value for the freshwater aquaculture of the whiteleg shrimp.

[0030] In one embodiment, the freshwater molting regulator for Penaeus vannamei is composed of the following raw materials in parts by weight.

[0031] 3 parts of calcium lactate, 0.01 parts of vitamin C, 0.02 parts of vitamin E, 5 parts of magnesium sulfate, 0.3 parts of arginine, 8 parts of dew grass, 7 parts of hawthorn, 7 parts of yeast powder, 15 parts of brown sugar, 6 parts of gynostemma pentaphyllum and 50 parts of rare earth.

[0032] In one embodiment, the rare earth is yttrium oxide.

[0033] Another embodiment of the present invention provides a method for preparing the above-mentioned white shrimp freshwater molting regulator, the preparation method comprising the following steps:

[0034] Drying and crushing the dew grass, Gynostemma pentaphyllum and Crataegus pinnatifida respectively;

[0035] Weigh each raw material according to weight, and mix the raw materials evenly to obtain the white shrimp freshwater molting regulator.

[0036] Yet another embodiment of the present invention provides a use of the above-mentioned Penaeus vannamei freshwater molting regulator in freshwater aquaculture of Penaeus vannamei.

[0037] Another embodiment of the present invention provides a freshwater culture method for Penaeus vannamei, the freshwater culture method for Penaeus vannamei comprising the following steps:

[0038] The whiteleg shrimp fry are put into the water body for breeding, and mixed feed is fed during the breeding process, wherein the mixed feed consists of the whiteleg shrimp freshwater molting regulator and special feed for shrimp.

[0039] In one embodiment, during the breeding process, the water pH is controlled to be 7.8-9.0, the dissolved oxygen is above 5 mg / L, and the temperature is 25-28°C.

[0040] In one embodiment, the shrimp-specific feed is fed 3 to 5 times a day during the breeding process, and the feeding amount each time is 80 to 120 g per 10,000 shrimps.

[0041] In one embodiment, after the white shrimp fry are released, the mixed feed is fed once every 3 to 4 days, and the feeding amount is 80 to 120 g / 10,000 shrimps; after 20 to 25 days of cultivation, the mixed feed is fed once every 6 to 8 days, and the feeding amount is 80 to 120 g / 10,000 shrimps.

[0042] In one embodiment, the mass ratio of the whiteleg shrimp freshwater molting regulator to the shrimp-specific feed in the mixed feed is 1:(90-110).

[0043] The technical solution of the present invention is further described in detail below through specific embodiments.

[0044] The shrimp feed used in the following embodiments is No. 0 feed for shrimp;

[0045] Example 1

[0046] This embodiment is a freshwater molting regulator for Penaeus vannamei, which is composed of the following raw materials in parts by weight.

[0047] 2 parts of calcium lactate, 0.02 parts of vitamin C, 0.01 parts of vitamin E, 6 parts of magnesium sulfate, 0.2 parts of arginine, 10 parts of dew grass, 6 parts of hawthorn, 10 parts of yeast powder, 10 parts of brown sugar, 8 parts of gynostemma pentaphyllum and 40 parts of yttrium oxide.

[0048] The preparation method of the above-mentioned white shrimp freshwater molting regulator comprises the following steps:

[0049] Drying and crushing the dew grass, Gynostemma pentaphyllum and Crataegus pinnatifida respectively;

[0050] Weigh each raw material according to weight, and mix the raw materials evenly to obtain the white shrimp freshwater molting regulator.

[0051] Example 2

[0052] This embodiment is a freshwater molting regulator for Penaeus vannamei, which is composed of the following raw materials in parts by weight.

[0053] 5 parts of calcium lactate, 0.01 parts of vitamin C, 0.02 parts of vitamin E, 4 parts of magnesium sulfate, 0.4 parts of arginine, 5 parts of dew grass, 8 parts of hawthorn, 5 parts of yeast powder, 20 parts of brown sugar, 5 parts of gynostemma pentaphyllum and 60 parts of yttrium oxide.

[0054] The preparation method of the above-mentioned white shrimp freshwater molting regulator comprises the following steps:

[0055] Drying and crushing the dew grass, Gynostemma pentaphyllum and Crataegus pinnatifida respectively;

[0056] Weigh each raw material according to weight, and mix the raw materials evenly to obtain the white shrimp freshwater molting regulator.

[0057] Example 3

[0058] This embodiment is a freshwater molting regulator for Penaeus vannamei, which is composed of the following raw materials in parts by weight.

[0059] 4 parts of calcium lactate, 0.02 parts of vitamin C, 0.02 parts of vitamin E, 5 parts of magnesium sulfate, 0.3 parts of arginine, 8 parts of dew grass, 7 parts of hawthorn, 8 parts of yeast powder, 15 parts of brown sugar, 7 parts of gynostemma pentaphyllum and 55 parts of yttrium oxide.

[0060] The preparation method of the above-mentioned white shrimp freshwater molting regulator comprises the following steps:

[0061] Drying and crushing the dew grass, Gynostemma pentaphyllum and Crataegus pinnatifida respectively;

[0062] Weigh each raw material according to weight, and mix the raw materials evenly to obtain the white shrimp freshwater molting regulator.

[0063] Example 4

[0064] This embodiment is a freshwater culture method for Penaeus vannamei, which comprises the following steps:

[0065] (a) The experiment was conducted at the Jinou Shrimp Industry Base, with a temperature between 26 and 30°C. The freshwater shrimp fry of white shrimp was provided by the Changsha College Industry-University-Research Base, and the salinity of the water used for transporting the shrimp fry was 0°C.

[0066] (b) Calcium hydroxide is used to adjust the pH value of the water environment to 8.5, an aerator is used to add oxygen, the dissolved oxygen in the water is above 5 mg / L, and a thermostat is used to adjust the water temperature to 28°C;

[0067] (c) 50,000 shrimp fry were placed in the pond for breeding, and No. 0 feed dedicated to shrimp was fed during the breeding period, 4 times a day, and 100 g / 10,000 shrimp fry were fed each time; after the white shrimp fry were placed, a mixed feed was fed once every 4 days, and the feeding amount was 100 g / 10,000 shrimp; after 20 days of breeding, a mixed feed was fed once every 8 days, and the feeding amount was 100 g / 10,000 shrimp, and the mixed feed consisted of the white shrimp freshwater molting regulator of Example 3 and No. 0 feed dedicated to shrimp in a mass ratio of 1:100.

[0068] After 3 months of breeding, the adult shrimps were weighed and 923 kilograms of whiteleg shrimp were obtained.

[0069] Comparative Example 1

[0070] This reference example is a freshwater culture method for Penaeus vannamei, which comprises the following steps:

[0071] (a) The culture was carried out at the Jinou Shrimp Base, with a temperature between 26 and 30°C, a pond area of ​​1 mu, and freshwater shrimp fry from the Changsha College Industry-University-Research Base. The salinity of the water used to transport the shrimp fry was 0 degrees.

[0072] (b) Calcium hydroxide is used to adjust the pH value of the water environment to 8.5, an aerator is used to add oxygen, the dissolved oxygen in the water is above 5 mg / L, and a thermostat is used to adjust the water temperature to 28°C;

[0073] (c) 50,000 shrimp fry were placed in the pond for breeding. During the breeding period, the shrimps were fed with feed No. 0, four times a day, with each feeding amount of 100 g per 10,000 shrimps. After the shrimp fry were placed, the shrimps were fed with feed No. 0 every four days, with a feeding amount of 100 g per 10,000 shrimps. After 20 days of breeding, the shrimps were fed with feed No. 0 every eight days, with a feeding amount of 100 g per 10,000 shrimps.

[0074] After 3 months of breeding, the adult shrimps were weighed and 686 catties of whiteleg shrimp were obtained.

[0075] Experimental example

[0076] This experimental example is a study on the effect of the freshwater molting regulator of Penaeus vannamei on shortening the molting cycle of Penaeus vannamei and improving the survival rate:

[0077] (1) The breeding experiment was divided into a control group and an experimental group. It was conducted at the Changsha College Industry-University-Research Base, with a temperature between 26 and 30 degrees Celsius and a breeding tank of approximately 0.3 m 3 The freshwater shrimp fry of South American white shrimp is provided by Changde Jiahua Aquatic Products, and the salinity of the water used for shrimp fry transportation is 0 degrees;

[0078] (2) Calcium hydroxide was used to adjust the pH value of the water environment to 8.5, an aerator was used to add oxygen, the dissolved oxygen in the water was above 5 mg / L, and a thermostat was used to adjust the water temperature to 28°C;

[0079] (c) 500 shrimp fry were raised in a culture tank and fed with No. 0 feed for shrimps during the culture period, four times a day, with 100 g / 10,000 shrimps each time;

[0080] Experimental group: After the release of white shrimp fry, the mixed feed was fed once every 4 days, and the feeding amount was 100g / 10,000 tails; after 20 days of cultivation, the mixed feed was fed once every 8 days, and the feeding amount was 100g / 10,000 tails, and the mixed feed consisted of the white shrimp freshwater molting regulator of Example 3 and shrimp-specific feed No. 0 in a mass ratio of 1:100;

[0081] Control group: After the release of white shrimp fry, feed No. 0 feed specially for shrimp once every 4 days, with a feeding amount of 100g / 10,000 shrimps; after 20 days of cultivation, feed No. 0 feed specially for shrimp once every 8 days, with a feeding amount of 100g / 10,000 shrimps.

[0082] The survival, growth and molting of shrimp were observed and recorded every day.

[0083] The results after 50 days of cultivation are as follows:

[0084] Table 1

[0085] project Experimental Group Control group <![CDATA[Cultivation volume (cm 3 )]]> 0.3 0.3 Stocking quantity (tails) 500 500 Survival rate% 92 90 Starting weight (g) 0.2±0.002 0.2±0.002 End weight (g) 8.3±0.5 6.1±0.5

[0086] Table 2

[0087] Molting cycle Experimental Group Control group Shrimp fry (less than 1g) 20～30h 30～40h Young shrimp (1-5g) 3-4 days 4-6 days Young shrimp (5-10g) 4-5 days 6-7 days Adult shrimp (10-15g) 7-8 days 10-12 days

[0088] From Table 1 and Table 2, we can see that:

[0089] The freshwater molting regulator for whiteleg shrimp of the present invention can improve the survival rate of the shrimp and shorten the molting period of the whiteleg shrimp. Within the same breeding time, the weight of a single shrimp is also improved.

[0090] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. A freshwater molting regulator for Penaeus vannamei, characterized in that The white shrimp freshwater molting regulator is composed of the following raw materials in parts by weight: 2-5 parts of calcium lactate, 0.01-0.02 parts of vitamin C, 0.01-0.02 parts of vitamin E, 4-6 parts of magnesium sulfate, 0.2-0.4 parts of arginine, 5-10 parts of dew grass, 6-8 parts of hawthorn, 5-10 parts of yeast powder, 10-20 parts of brown sugar, 5-8 parts of Gynostemma pentaphyllum and 40-60 parts of rare earth.

2. The freshwater molting regulator for Penaeus vannamei according to claim 1, characterized in that The white shrimp freshwater molting regulator is composed of the following raw materials in parts by weight: 4 parts of calcium lactate, 0.02 parts of vitamin C, 0.02 parts of vitamin E, 5 parts of magnesium sulfate, 0.3 parts of arginine, 8 parts of dew grass, 7 parts of hawthorn, 8 parts of yeast powder, 15 parts of brown sugar, 7 parts of gynostemma pentaphyllum and 55 parts of yttrium oxide.

3. The freshwater molting regulator for Penaeus vannamei according to claim 1, characterized in that The rare earth is yttrium oxide.

4. The method for preparing the freshwater molting regulator of Penaeus vannamei according to any one of claims 1 to 3, characterized in that: The steps include: Drying and crushing the dew grass, Gynostemma pentaphyllum and Crataegus pinnatifida respectively; Weigh each raw material according to weight, and mix the raw materials evenly to obtain the white shrimp freshwater molting regulator.

5. Use of the freshwater molting regulator of Penaeus vannamei according to any one of claims 1 to 3 in freshwater aquaculture of Penaeus vannamei.

6. A freshwater culture method for Penaeus vannamei, characterized in that: The steps include: The whiteleg shrimp fry are put into a water body for breeding, and mixed feed is fed during the breeding process, wherein the mixed feed is composed of the whiteleg shrimp freshwater molting regulator according to any one of claims 1 to 3 and special feed for shrimp.

7. The freshwater culture method of Penaeus vannamei according to claim 6, characterized in that: During the breeding process, the pH value of the water body is controlled to be 7.8-9.0, the dissolved oxygen is above 5 mg / L, and the temperature is 25-28°C.

8. The freshwater culture method of Penaeus vannamei according to claim 6, characterized in that: During the breeding process, the shrimp-specific feed is fed 3 to 5 times a day, and the feeding amount each time is 80 to 120 g per 10,000 shrimps.

9. The freshwater culture method of Penaeus vannamei according to claim 6, characterized in that: After the white shrimp fry are released, the mixed feed is fed once every 3 to 4 days, and the feeding amount is 80 to 120 g / 10,000 shrimps; after 20 to 25 days of cultivation, the mixed feed is fed once every 6 to 8 days, and the feeding amount is 80 to 120 g / 10,000 shrimps.

10. The freshwater culture method of Penaeus vannamei according to claim 1, characterized in that: The mass ratio of the whiteleg shrimp freshwater molting regulator to the shrimp-specific feed in the mixed feed is 1:(90-110).