Method for effectively inhibiting propagation of fruit fly larvae in nutrient soil during arabidopsis thaliana planting

Abstract

The invention discloses a method for effectively inhibiting propagation of fruit fly larvae in nutrient soil during arabidopsis thaliana planting, and relates to the related field of soil science. The method comprises the following steps: (1) planting arabidopsis thaliana seedlings; (2) preparing a non-absorbent material; (3) using a non-absorbent material; (4) watering in the tray. Compared with the prior art, the method has the following advantages and positive effects that the non-absorbent material is prepared to maintain the continuous dryness of the surface layer nutrient medium, and fruit flies can be effectively inhibited from breeding on the surface layer of the nutrient soil when the arabidopsis thaliana is planted, so that the arabidopsis thaliana seedlings are prevented from being gnawed by fruit fly larvae and even the whole plant is prevented from being gnawed to die; the outbreak of soil mould on the surface layer can be effectively inhibited, and scientific researchers taking arabidopsis thaliana as experimental materials can be assisted to efficiently cultivate plant materials capable of being used for measuring experimental data in a greenhouse in a labor-saving manner.

Description

Technical Field

[0001] The invention relates to soil science related fields, and in particular to a method for effectively inhibiting the reproduction of fruit fly larvae in nutrient soil when Arabidopsis thaliana is planted. Background Art

[0002] Arabidopsis thaliana is a model plant for genetic research. When Arabidopsis is grown in a greenhouse, it will produce various diseases and pests, among which fruit fly pests are the most common. How to effectively inhibit the nibbling of Arabidopsis by fruit fly larvae is an important problem that researchers who use Arabidopsis as experimental materials urgently need to solve. Adult fruit flies are small flying insects that lay eggs on the surface of the Arabidopsis substrate. After the eggs hatch, they become transparent white insects and mainly nibble on the Arabidopsis leaves close to the surface substrate. The humid environment on the surface of the substrate is conducive to the hatching of fruit fly eggs. Once it is not controlled, the fruit fly larvae will multiply in large numbers on the surface of the substrate, and the entire Arabidopsis seedling can be eaten up. At present, the methods for controlling the reproduction of fruit flies in the surface substrate of Arabidopsis seedlings mainly rely on sticky fly stickers to capture adults, adding pesticides to the substrate; sprinkling vermiculite on the surface of the substrate to control the moisture of the topsoil; shoveling away the topsoil of the Arabidopsis substrate with fruit fly larvae; reducing the amount of watering; spraying pesticides, etc. However, although these methods are effective, it is difficult to inhibit the reproduction of fruit flies in Arabidopsis nutrient soil, thus affecting the accurate collection of experimental data.

[0003] So far, there is no report in the prior art on a method for effectively inhibiting the reproduction of Drosophila in Arabidopsis thaliana without spraying pesticides. Summary of the invention

[0004] The purpose of the present invention is to overcome the problems and shortcomings of the prior art and provide a simple and effective method for inhibiting the reproduction of fruit fly larvae in the nutrient soil when Arabidopsis is planted, that is, inhibiting the reproduction of fruit fly larvae on the surface of the nutrient soil, avoiding the occurrence of Arabidopsis seedlings being eaten by fruit fly larvae or even the entire plant being eaten up, thereby contributing to scientific research with Arabidopsis as the research object.

[0005] The object of the present invention is achieved in that:

[0006] The method comprises the following steps:

[0007] ① Planting of Arabidopsis seedlings

[0008] Arabidopsis seedlings at the 2-leaf age were transplanted into fresh substrates that did not contain Drosophila eggs and grown in an Arabidopsis room for 7-10 days;

[0009] ②Preparation of non-absorbent materials

[0010] Pinstrup (PINDSTRUP) Danish Pinstrup's imported moss peat soil is one of the nutrient substrates commonly used in laboratories for cultivating Arabidopsis thaliana. Take an appropriate amount of this nutrient substrate, rub it into small particles by hand, and place it in an oven at 85°C to remove moisture from the substrate. The substrate becomes a non-absorbent material. Fill the substrate into a nutrient pot, put it in a tray, and pour water into the tray. The substrate in the nutrient pot cannot naturally absorb water from bottom to top.

[0011] ③Use of non-absorbent materials

[0012] The Arabidopsis thaliana was grown in the Arabidopsis room for 7-10 days. At this time, the Arabidopsis thaliana had been planted and had 4-6 leaves. The surface of the nutrient soil where the Arabidopsis thaliana was grown was covered with the prepared non-absorbent material to a thickness of about 0.5-1 cm. The Arabidopsis thaliana leaves covered with the non-absorbent material were released by gently shaking the tray or manually.

[0013] ④Watering in the tray

[0014] Place the small pots where Arabidopsis thaliana grows in a tray; later, water is poured into the tray and the nutrient soil absorbs the water naturally, which ensures that the non-absorbent material on the surface continues to dry, thereby maintaining a dry environment on the surface of the nutrient soil where Arabidopsis thaliana grows.

[0015] Working principle:

[0016] The seedlings transplanted into the nutrient soil and grown for 7-10 days have been established, the leaves have reached 4-6 pieces, and the whole plant has a certain height. The leaves can be covered with a non-absorbent material of about 0.5-1 cm below the leaves, and the covering will not affect the normal growth of the Arabidopsis seedlings; by laying a layer of non-absorbent material on the surface matrix of the nutrient soil for planting Arabidopsis, on the one hand, a small amount of fruit fly eggs that may be produced before the non-absorbent material is laid will die due to the dry environment during the hatching process of the larvae, and on the other hand, the eggs produced on the non-absorbent material in the subsequent growth process cannot hatch due to the lack of a moist environment, thereby inhibiting the hatching of fruit flies on the surface of the nutrient soil when Arabidopsis is planted, and effectively controlling the reproduction of fruit flies in the Arabidopsis room.

[0017] The purpose of this method:

[0018] The method can be used to prevent and inhibit the reproduction of fruit flies in Arabidopsis chambers.

[0019] The present invention has the following advantages and positive effects:

[0020] The method prepares a non-absorbent material and maintains the continuous drying of the surface nutrient matrix, which can effectively inhibit the reproduction of fruit flies on the surface of the nutrient soil when Arabidopsis is planted, thereby preventing the Arabidopsis seedlings from being eaten by fruit fly larvae or even the entire plant from being eaten and killed, and can also effectively inhibit the outbreak of mold on the surface soil.

[0021] It helps researchers who use Arabidopsis as experimental material to efficiently and labor-savingly cultivate plant materials that can be used to measure experimental data in the greenhouse. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a flow chart of inhibiting the reproduction of fruit flies in nutrient soil when Arabidopsis is planted. In the figure:

[0023] 1—Transplant the seedlings into fresh substrate;

[0024] 2—Preparation of non-absorbent materials;

[0025] 3—Cover the seedlings with non-absorbent material 7-10 days after transplanting;

[0026] 4—Water is poured into the tray for natural water absorption.

[0027] Figure 2 This is the growth state of Arabidopsis thaliana covered and not covered with non-absorbent materials in the topsoil. In the figure:

[0028] a—Arabidopsis thaliana planted in a Drosophila infested Arabidopsis thaliana room, with the top two pots covered with non-absorbent material, and the bottom two pots not covered with non-absorbent material;

[0029] b—is an enlarged view of the surface layer that is not covered with non-absorbent material. Many white transparent fruit fly larvae can be seen, and the light green residues are the stem segments left after the fruit fly larvae have eaten the Arabidopsis seedlings. DETAILED DESCRIPTION

[0030] The following is a detailed description with reference to the accompanying drawings and embodiments:

[0031] ① Planting of Arabidopsis seedlings

[0032] Arabidopsis seedlings grown on MS plates for about 4 days were transplanted to fresh substrates without Drosophila eggs, watered with sufficient water, and grown in Arabidopsis chambers for 7-10 days ( Figure 1 ).

[0033] ②Preparation of non-absorbent materials

[0034] Take an appropriate amount of Danish Pin's imported moss peat soil, rub it into small pieces by hand, and bake it in an oven at 85℃ for at least 3 hours to remove the moisture in the substrate. When the substrate is completely dry and the temperature drops to room temperature, put it into a clean plastic sealed bag for later use ( Figure 1 ).

[0035] ③Use of non-absorbent materials

[0036] The Arabidopsis thaliana grown in the Arabidopsis room for 7-10 days has been established and has 4-6 leaves. The entire surface of the nutrient soil for growing Arabidopsis thaliana is covered with the prepared non-absorbent material to a thickness of about 0.5-1 cm. The Arabidopsis thaliana leaves covered with the non-absorbent material are released by gently shaking the tray or manually. Figure 1 ); If the Arabidopsis room with a fruit fly outbreak is not controlled, such as Figure 2 The transplanted Arabidopsis seedlings were severely eaten until the entire plant was eaten up.

[0037] ④Watering in the tray

[0038] Pour an appropriate amount of water into the tray containing the small pots of Arabidopsis thaliana. The dry substrate on the surface does not actively absorb water, and the dry environment on the surface is maintained continuously ( Figure 1 ); in the later stage, the tray is continuously filled with water, and the nutrient soil under the non-absorbent material absorbs water naturally, which can keep the non-absorbent material on the surface of Arabidopsis thaliana continuously dry throughout the growth period, thereby maintaining a dry environment on the surface of the Arabidopsis growth matrix, and effectively inhibiting the reproduction of fruit flies in the Arabidopsis room.

Claims

1. A method for inhibiting the reproduction of fruit fly larvae in nutrient soil when Arabidopsis thaliana is planted, characterized in that The following steps are involved: ① Planting of Arabidopsis seedlings Arabidopsis seedlings at the 2-leaf age were transplanted into fresh substrate that did not contain Drosophila eggs and grown in an Arabidopsis room for 7-10 days; ②Preparation of non-absorbent materials Pin's Matrix Denmark Pin's imported moss peat soil is one of the nutrient matrices commonly used in laboratories to cultivate Arabidopsis; take an appropriate amount of this nutrient matrix, rub it into small particles by hand, put it in an oven at 85°C to remove moisture from the matrix, and the matrix becomes a non-absorbent material. Fill the matrix into a nutrient pot, put it in a tray, and pour water into the tray. The matrix in the nutrient pot cannot naturally absorb water from bottom to top. ③Use of non-absorbent materials The Arabidopsis thaliana was grown in the Arabidopsis room for 7-10 days. At this time, the Arabidopsis thaliana had been planted and had 4-6 leaves. The surface of the nutrient soil where the Arabidopsis thaliana was grown was covered with the prepared non-absorbent material to a thickness of about 0.5-1 cm. The Arabidopsis thaliana leaves covered with the non-absorbent material were released by gently shaking the tray or manually. ④Watering in the tray Place the small pots where Arabidopsis thaliana grows in a tray; later, water is poured into the tray and the substrate absorbs the water naturally, which ensures that the non-absorbent material on the surface continues to dry, thereby maintaining a dry environment on the surface of the Arabidopsis thaliana growth substrate.

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