Visual motion response testing device for zebra fish behavior research

Abstract

The invention discloses a visual motion response testing device for zebra fish behavior research, which comprises a shading box body, shading cloth is laid on the inner wall of the shading box body, a circular cavity is formed in the top of the shading box body, and a cylindrical zebra fish container is arranged in the circular cavity; a step is arranged at the top end of the cylindrical zebra fish container, the step is connected with the top end of the shading box body, an opening of the cylindrical zebra fish container faces upwards, and the cylindrical zebra fish container is arranged in the center of the hollow cylindrical grating; according to the invention, the shading box is matched with the shading cloth, the hollow cylindrical grating, the illumination assembly and the acquisition assembly to detect and observe the behavioral change condition of the zebra fish under the condition of illumination or not during an experiment, and the zebra fish can be detected and observed under different illumination intensities and under the same illumination intensity and illumination time. Under the rotation speed and direction of the grating, the zebra fish behavioral change condition is experimented, the variable control principle is met, repeated experiments are avoided, the experiment period is shortened, and the experiment efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of visual-motor reaction devices, and in particular to a visual-motor reaction testing device used for zebrafish behavior research. Background Art

[0002] As a unique small model animal, zebrafish has many excellent and unique characteristics, such as small size, genetic composition similar to humans, optically transparent body of juvenile fish for easy observation, rapid growth and reproduction, and advantages such as eye movement response and optokinetic response. The optokinetic response refers to the behavior of zebrafish chasing the target when the target moves. This response can be used to detect vision-related gene mutations in juveniles and adult fish. By screening individuals who are slow to respond to changes in the direction of the grating, individuals with visual gene mutation defects can be screened.

[0003] However, the traditional visual-motor reaction test device has deficiencies in accuracy and flexibility. Its light source, zebrafish container and rotating grating size are fixed, making it difficult to modify or improve according to the actual needs of users, and unable to meet the high standards of modern scientific research. In addition, the traditional device only considers the test conditions of normal lighting, not low-light conditions, and lacks exploration of zebrafish visual-motor reactions in low-light environments. The camera used in the traditional visual-motor reaction test device performs poorly in low-light environments, which limits the accuracy and universality of the test results to a certain extent.

[0004] Therefore, it is urgent to develop a highly adjustable and versatile visual-motor response testing device, especially one that can maintain a high accuracy of zebrafish behavior analysis under low light conditions, which is of great significance for further in-depth and comprehensive understanding of the visual-motor response mechanism of zebrafish. Summary of the invention

[0005] The purpose of the present invention is to provide a visual-motor reaction testing device for zebrafish behavior research, so as to solve the problems that the existing visual-motor reaction testing devices have fixed light sources, zebrafish containers and rotating grating sizes, or lack exploration of zebrafish visual-motor reactions in weak light environments, are difficult to modify or improve according to actual user needs, and cannot meet the high standards of modern scientific research.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A visual-motor reaction test device for zebrafish behavior research, comprising a light-shielding box, the inner wall of which is covered with a light-shielding cloth, a circular hole is provided on the top of the light-shielding box, and a cylindrical zebrafish container is arranged in the circular hole;

[0008] A step is arranged at the top of the cylindrical zebrafish container, the step is connected to the top of the light shielding box, the cylindrical zebrafish container is arranged with its opening facing upward, and the cylindrical zebrafish container is placed in the center of the hollow cylindrical grating;

[0009] The hollow cylindrical grating is placed on a turntable, the bottom end of the turntable is connected to the output end of the motor, the motor is arranged on a turntable bracket, and the turntable bracket is arranged at the bottom end of the light shielding box;

[0010] A lighting component is arranged inside the light-shielding box, and the lighting component is arranged on the outer ring of the hollow cylindrical grating;

[0011] A collection component is arranged above the light-shielding box, and the collection component and the opening of the cylindrical zebrafish container are on the same center line.

[0012] Optionally, the lighting assembly includes an iron frame, which is arranged in the light-shielding box. A plurality of beaker clamps are arranged on the iron frame, and brightness-adjustable annular LED lamps are correspondingly arranged on the beaker clamps. The annular LED lamps are arranged around the outside of the hollow cylindrical grating.

[0013] Optionally, the acquisition component includes an image acquisition device bracket, which is arranged on the top of the light-shielding box, and a near-infrared camera is arranged on the image acquisition device bracket, and the near-infrared camera is arranged directly above the opening of the cylindrical zebrafish container.

[0014] Optionally, the number of the annular LED lamp tubes is at least two, and 6500K brightness-adjustable annular LED lamp tubes are used.

[0015] The beneficial effects of the present invention are as follows: the behavioral changes of zebrafish with or without light during the detection and observation experiment are realized by using a shading box in conjunction with a shading cloth, a hollow cylindrical grating, a lighting component, and a collection component; the behavioral changes of the experimental zebrafish are detected under different light intensities, and under the same light intensity and light time, the speed and direction of the grating rotation, and the principle of controlled variables is met, repeated experiments are avoided, the experimental cycle is shortened, and the experimental efficiency is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The present invention will now be described by way of example with reference to the accompanying drawings, in which:

[0017] Figure 1 It is a schematic diagram of the structure of the present invention;

[0018] Figure 2 It is a schematic diagram of the cylindrical zebrafish container, the light-shielding box, the brightness-adjustable annular LED lamp tube, the hollow cylindrical grating, and the turntable packaging structure of the present invention;

[0019] Figure 3It is a schematic diagram of the experimental effect of the present invention;

[0020] Figure 4 This is a collection diagram of the experimental detection process of the present invention in a weak light environment;

[0021] Figure 5 It is a diagram of the movement trajectory of zebrafish during the experiment in a weak light environment of the present invention.

[0022] In the figure: 1. shading box; 2. shading cloth; 3. cylindrical zebrafish container; 4. near-infrared camera; 5. image acquisition device bracket; 6. iron stand; 7. ring-shaped LED light tube; 8. motor; 9. turntable; 10. turntable bracket; 11. beaker clamp; 12. hollow cylindrical grating. DETAILED DESCRIPTION

[0023] Any feature disclosed in this specification, unless otherwise stated, can be replaced by other equivalent or alternative features with similar purposes. That is, unless otherwise stated, each feature is only an example of a series of equivalent or similar features.

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] In the description of the invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0026] In the description of the invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, welding, riveting, bonding, etc., or a detachable connection, threaded connection, key connection, pin connection, etc., or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0027] like Figure 1-5 The visual-motor reaction test device for zebrafish behavior research shown in the figure comprises a light-shielding box 1, the inner wall of the light-shielding box 1 is covered with a light-shielding cloth 2, a circular hole is opened on the top of the light-shielding box 1, and a cylindrical zebrafish container 3 is arranged in the circular hole;

[0028] A step is arranged at the top of the cylindrical zebrafish container 3, and the step is connected to the top of the light shielding box 1. The cylindrical zebrafish container 3 is arranged with its opening facing upward, and the cylindrical zebrafish container 3 is placed in the center of the hollow cylindrical grating 12;

[0029] The hollow cylindrical grating 12 is placed on the turntable 9, the bottom end of the turntable 9 is connected to the output end of the motor 8, the motor 8 is arranged on the turntable bracket 10, and the turntable bracket 10 is arranged at the bottom end of the light shielding box 1;

[0030] A lighting component is arranged in the light-shielding box 1, and the lighting component is arranged on the outer ring of the hollow cylindrical grating 12;

[0031] A collection component is arranged above the light-shielding box 1, and the collection component is on the same center line as the opening of the cylindrical zebrafish container 3. It should be noted that the cylindrical zebrafish container 3 can replace the experimental object or increase or decrease the water level at any time. The hollow cylindrical grating 12 is composed of alternating black and white vertical stripes and is placed in the center of the turntable 9. The turntable 9 drives the rotation of the hollow cylindrical grating 12, and the light passing through the hollow cylindrical grating 12 is constantly changing, and the zebrafish will make corresponding visual-motion reactions; the light-shielding cloth 2 can block external light, creating an environment without external light source interference for the entire experimental device;

[0032] The motor 8 drives the turntable 9 to run at different speeds and directions, thereby driving the hollow cylindrical grating 12 to rotate to achieve the desired light angle change.

[0033] Specifically, the lighting assembly includes an iron frame 6, which is arranged in the light-shielding box 1, and a plurality of beaker clamps 11 are arranged on the iron frame 6. The beaker clamps 11 are correspondingly provided with brightness-adjustable annular LED lamp tubes 7, and the annular LED lamp tubes 7 are arranged around the outside of the hollow cylindrical grating 12. It should be noted that each annular LED lamp tube 7 has a separate switch power line for separate control, and the beaker clamps 11 can be moved up and down to change the position of the annular LED lamp tube 7, thereby changing the lighting angle, and achieving different lighting intensities in the experimental environment by changing the number and brightness of the annular LED lamp tubes 7.

[0034] Specifically, the acquisition component includes an image acquisition bracket 5, which is arranged at the top of the light shielding box 1, and a near-infrared camera 4 is arranged on the image acquisition bracket 5, and the near-infrared camera 4 is arranged just above the opening of the cylindrical zebrafish container 3. It should be noted that the near-infrared camera 4 recognizes fishes by adding an infrared channel on the basis of the three channels of red, green and blue, and combines the computer vision deep learning algorithm to improve the clarity of the captured video; the acquisition component subsequently adopts one of the target tracking algorithms, namely TrackFormer, in terms of recognition processing; it is a target tracking algorithm based on Transformer, which aims to improve the accuracy and robustness of target tracking by using the self-attention mechanism of the Transformer architecture.

[0035] After TrackFormer obtains the continuous image frame sequence of zebrafish, it preprocesses the original zebrafish images, including denoising, contrast enhancement and other operations, so as to better extract features. The input zebrafish image frame is divided into multiple small blocks using the Transformer architecture, and each small block is regarded as a feature vector. The degree of association between each small block and other small blocks is calculated to extract the global and local features of the image. In the initial frame, TrackFormer needs to detect the position and bounding box of the zebrafish. In subsequent frames, TrackFormer will match the features of the current frame with the features of the zebrafish tracked in the previous frame. By calculating the similarity between the features, it is determined which areas in the current frame are associated with the previous zebrafish target. This feature matching process can effectively cope with the influence of factors such as morphological changes and lighting changes of zebrafish. According to the results of feature matching, the tracking trajectory of the zebrafish is updated. At the same time, some filtering algorithms or prediction models are used in the post-processing stage to smooth and predict the tracking results to improve the accuracy and stability of tracking. Thus, the final recognition and detection results of the target zebrafish are obtained.

[0036] Specifically, at least two annular LED lamp tubes 7 are provided, and a 6500K brightness adjustable annular LED lamp tube is used. It should be noted that the two annular LED lamp tubes 7 are respectively controlled by separate switch lines to adjust the experimental light intensity. If other lighting conditions are required, different similar lamp tubes can be flexibly replaced to meet the light source requirements.

[0037] The working principle of this specific embodiment is as follows: put the cylindrical zebrafish container 3 into the light-shielding box 1, adjust the number and brightness of the annular LED lamp tube 7 and the fixed position of the beaker clamp 11 through the power switch line, so that the light intensity meets the experimental requirements, and inject a proper amount of water into the cylindrical zebrafish container 3, then place the zebrafish seedlings in the cylindrical zebrafish container 3, let it stand for ten minutes to adapt to the current environment, and after ten minutes, the motor 8 works, starts the turntable 9, drives the hollow cylindrical grating 12 to rotate for fifteen minutes and then stops;

[0038] The above process transmits the collected zebrafish image information to the computer for step-by-step analysis. Finally, the computer obtains a schematic diagram of the algorithm recognition results through algorithm analysis. The corresponding behavioral action response shown by the zebrafish's visual-motor response can detect the zebrafish's visual response, thereby screening out zebrafish with relatively slow responses in the group, and then selecting individuals with zebrafish visual defects, thereby improving the optimization of zebrafish varieties.

[0039] The present invention is not limited to the above-mentioned specific embodiments, but extends to any new features or any new combination disclosed in this specification, as well as any new method or process steps or any new combination disclosed.

Claims

1. A visual-motor reaction test device for zebrafish behavior research, characterized in that: It comprises a light-shielding box, the inner wall of which is covered with a light-shielding cloth, a circular hole is provided on the top of the light-shielding box, and a cylindrical zebrafish container is arranged in the circular hole; A step is arranged at the top of the cylindrical zebrafish container, the step is connected to the top of the light shielding box, the cylindrical zebrafish container is arranged with its opening facing upward, and the cylindrical zebrafish container is placed in the center of the hollow cylindrical grating; The hollow cylindrical grating is placed on a turntable, the bottom end of the turntable is connected to the output end of the motor, the motor is arranged on a turntable bracket, and the turntable bracket is arranged at the bottom end of the light shielding box; A lighting component is arranged inside the light-shielding box, and the lighting component is arranged on the outer ring of the hollow cylindrical grating; A collection component is arranged above the light-shielding box, and the collection component and the opening of the cylindrical zebrafish container are on the same center line.

2. The visual-motor reaction testing device for zebrafish behavior research according to claim 1, characterized in that: The lighting assembly includes an iron frame, which is arranged in the light-shielding box. A plurality of beaker clamps are arranged on the iron frame, and brightness-adjustable annular LED lamps are arranged on the beaker clamps accordingly. The annular LED lamps are arranged around the outside of the hollow cylindrical grating.

3. The visual-motor reaction testing device for zebrafish behavior research according to claim 1, characterized in that: The acquisition component comprises an image acquisition device bracket, which is arranged at the top of the light-shielding box body. A near-infrared camera is arranged on the image acquisition device bracket, and the near-infrared camera is arranged just above the opening of the cylindrical zebrafish container.

4. The visual-motor reaction testing device for zebrafish behavior research according to claim 2, characterized in that: The number of the annular LED lamp tubes is at least two, and 6500K brightness adjustable annular LED lamp tubes are used.

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