Method for industrially cultivating bumblebees

Abstract

The invention provides a method for industrially cultivating bumblebees, and belongs to the technical field of insect breeding. The method comprises the six steps of resuscitation of hibernation queen bees, induction of the queen bees to lay eggs, cultivation of mature bee colonies, cultivation of queen bees and male bumblebees, mating of the queen bees and induction of hibernation of the queen bees. According to the method, the bumblebee colony can be cultivated indoors all the year round by utilizing an indoor means, so that the breeding efficiency and yield of the bumblebees are improved, the health of the bumblebees can be ensured, the factory-like cultivation of the bumblebee colony is realized, and stable and efficient pollination service is provided for agricultural production; especially, when bee eggs and accompanying bees are provided for new queen bees in the egg laying period at the same time, it is found that the egg laying rate of the new queen bees can be remarkably increased, and the egg laying rate can reach 89%. According to the method, the whole breeding period is three months when bumblebee swarms are bred, and according to the method, thirty thousand groups of bumblebees can be bred in a factory building of one thousand square meters every year.

Description

Technical Field

[0001] The invention belongs to the technical field of insect breeding, and in particular relates to a method for factory-based breeding of bumblebees. Background Art

[0002] Bumblebees are insects of the genus Bombus, family Apidae, order Hymenoptera. They are named for their large, hairy bodies and bear-like appearance. Bumblebees are distributed throughout the world and are most common in temperate zones. There are no less than 150 species of bumblebees in China. Bumblebees are extremely abundant in Xinjiang and Northeast China, but there are very few bumblebees on the plains in southern and southwestern China. Bumblebees are beneficial insects and have a certain effect on the pollination of agricultural and forestry crops, forage, Chinese herbal medicines and wild plants, especially for the pollination of forage. Using bumblebees to pollinate greenhouse vegetables has a high pollination rate and low cost, which can increase yields and improve the quality of fruits and vegetables. The fruits pollinated by bumblebees have good fruit shape and fewer deformed fruits. It can also solve the problem of hormone pollution caused by chemical pollination and has high economic value.

[0003] Bumblebees live in a social environment. The nest includes a large female bee (queen bee), male bees, and a large number of small worker bees. The female bees are responsible for laying eggs and secreting wax to build the nest, the male bees are responsible for mating, and the worker bees are responsible for collecting nectar, pollen, and taking care of the larvae. Bumblebees are a good indicator animal of the natural environment and are meaningful for the study of animals and physical geography. Bumblebees can now be raised artificially.

[0004] However, in the traditional artificial breeding method of bumblebees, the cultivation of bumblebees mainly depends on the outdoor natural environment. Its reproductive cycle and reproduction efficiency are closely related to the climate environment. Especially in cold or unfavorable seasons, the reproduction of bumblebees is severely restricted. In winter, bumblebees cannot reproduce effectively, and it is impossible to ensure that there are enough bumblebees to supply agricultural production all year round. In addition, for the cultivation of bumblebees, the traditional outdoor method relies on natural food and environment, which is greatly affected by the external environment and it is difficult to always maintain a stable population quantity and quality.

[0005] With the continuous development of modern agriculture, the demand for agricultural pollination is increasing, especially in protected cultivation, where bumblebees are increasingly used as efficient natural pollinators. In order to meet this demand, the use of factory-based and indoor methods to breed bumblebees has become an inevitable choice. Therefore, it is necessary to develop a method for breeding bumblebees in an indoor controlled environment all year round, which can not only improve the reproductive efficiency and yield of bumblebees, but also ensure the health of bumblebees, thereby providing stable and efficient pollination services for agricultural production. Summary of the invention

[0006] The present invention aims to provide a method for factory-based breeding of bumblebees. The method can breed bumblebee colonies indoors all year round by utilizing indoor means, which not only improves the reproductive efficiency and yield of bumblebees, but also ensures the health of bumblebees, thereby realizing the factory-based breeding of bumblebee colonies and providing stable and efficient pollination services for agricultural production.

[0007] In order to achieve the above object, the present invention adopts the following technical solution.

[0008] The present invention provides a method for factory-based breeding of bumblebees, comprising:

[0009] (1) Resuscitation of hibernating queen bees

[0010] The queen bee which hibernates for 3-6 months is taken out from the hibernation chamber, placed in an environment with a room temperature of 14-18°C for 12-72 hours, and fed with pollen and 45-55wt% sucrose water;

[0011] (2) Inducing the queen bee to lay eggs

[0012] After the queen bee recovers, each queen bee is placed in an egg-laying box, and the humidity of the environment is controlled to be 30%-70% and the temperature is 23-28°C. At this time, the queen bee is fed with mixed pollen. If the queen bee does not lay eggs, a new pollen mass is replaced every 2-5 days, and 45-55wt% sucrose water is fed at the same time;

[0013] (3) Cultivation of mature bee colonies

[0014] When the bee colony develops into a colony strength of 20-30 worker bees, the entire bee colony is moved to a bee box for further breeding, and the humidity of the environment is controlled to be 30%-70% and the temperature is 22-26°C. At this time, the bee colony is fed with northern pollen, a new pollen ball is added every day, and 45-55wt% sucrose water is fed at the same time, and the bee colony is cultivated until the bee colony develops into a colony strength of 80-120 worker bees;

[0015] (4) Breeding of virgin queens and male bumblebees

[0016] Select a bee colony with a large number of worker bees and eggs from the mature bee colonies cultivated in step (3) and continue to cultivate it, maintain the same environmental temperature and feeding conditions, and cultivate it for 28-42 days. The bee colony will produce new virgin queens and male bumblebees;

[0017] (5) Mating of the Virgin Queen

[0018] Select 6-10 day old virgin queens and 3-7 day old male bumblebees and place them in mating cages, place 200-400 virgin queens and 400-800 male bumblebees in each mating cage, control the humidity of the mating room to 30%-70%, the temperature to 22-24° C., strong light, ventilation, place a number of pollen balls and a portion of 45-55wt% sucrose water in each mating cage, and keep the virgin queens and male bumblebees together in the mating cages for 4-8 days;

[0019] (6) Inducing Queen Bee to Hibernate

[0020] After mating is completed, place the mating cage in an environment of 14-18℃ and raise it for 12-72h. After 12-72h, pick out the queen bee and place it in the hibernation room. Control the humidity of the hibernation room to 80%-95% and the temperature to 1-6℃. When it is necessary to cultivate a new bee colony, revive the hibernating queen bee.

[0021] Furthermore, in step (2), the mixed pollen is a mixed powder prepared by mixing northern gentian pollen, rapeseed pollen and peach pollen in a weight ratio of (0.8-1.2):(0.8-1.2):(0.8-1.2).

[0022] Furthermore, in step (2), 2-3 bee eggs and 1-3 companion bees are added to each egg-laying box.

[0023] Furthermore, the length, width and height of the egg-laying box are 12cm×8cm×8cm.

[0024] Furthermore, the length, width and height of the adult bee box are 28cm×28cm×24cm.

[0025] Furthermore, the mating cage is 1m 3 Breathable mesh cage.

[0026] Compared with the prior art, the beneficial technical effects of the present invention are:

[0027] 1. The present invention can cultivate bumblebee colonies indoors throughout the year by utilizing indoor means, which not only improves the reproductive efficiency and yield of bumblebees, but also ensures the health of bumblebees, thereby realizing the factory-based cultivation of bumblebee colonies and providing stable and efficient pollination services for agricultural production.

[0028] 2. The inventors found that by providing bee eggs and companion bees to new queen bees in the egg-laying period, the egg-laying rate can be significantly improved, and the egg-laying rate can reach 89%.

[0029] 3. The entire breeding cycle of the bumblebee colony cultivated by the method of the present invention is three months. According to this method, 30,000 bumblebee colonies can be cultivated every year in a factory building of 1,000 square meters. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1This is a flow chart of factory-based breeding of bumblebees in Example 1 of the present invention;

[0031] Figure 2 The appearance diagram of the bumblebee queen and the egg-laying box raised in the egg-laying box in Example 1 of the present invention, wherein AC is the bumblebee in the egg-laying box, and D is the appearance diagram of the egg-laying box;

[0032] Figure 3 The following are the appearance diagrams of the bumblebee colonies and the adult bee boxes raised in Example 1 of the present invention, wherein AC is the colonies in the adult bee boxes, and D is the appearance diagram of the adult bee boxes;

[0033] Figure 4 The mating cage and the bumblebees raised in the mating cage in Example 1 of the present invention, wherein A is an appearance diagram of the mating cage, and B is a bumblebee raised in the mating cage;

[0034] Figure 5 This is the hibernation chamber and the queen bee hibernating in Example 1 of the present invention, wherein AB is the appearance of the hibernation chamber, C is the appearance of the hibernation box, and D is the queen bee hibernating in the hibernation box. DETAILED DESCRIPTION

[0035] The technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings and embodiments.

[0036] Example 1

[0037] Please see attached Figure 1 As shown, the method for factory-based breeding of bumblebees in this embodiment includes six steps: reviving hibernating queen bees, inducing queen bees to lay eggs, breeding of mature bee colonies, breeding of virgin queens and male bumblebees, mating of virgin queens, and inducing queen bees to hibernate. Through the cycle of these six steps, it is possible to achieve continuous indoor breeding of bumblebee colonies throughout the year, thereby realizing factory-based breeding of bumblebee colonies.

[0038] (1) Resuscitation of hibernating queen bees: Queen bees that have hibernated for 3 months are taken out of the hibernation chamber, placed in a room temperature of 16°C for 12 hours, and fed with pollen and 50 wt% sucrose water. Since queen bees that hibernate for less than three months have a low egg-laying rate, queen bees that hibernate for more than three months are selected.

[0039] (2) Inducing queen bee to lay eggs: Each queen bee after resuscitation is placed in an egg-laying box, and the humidity of the environment is controlled to be 30% and the temperature is controlled to be 23°C. At this time, the queen bee is fed with a mixed powder of northern pollen, rapeseed powder and peach pollen in a weight ratio of 0.8:1.2:1.2. If the queen bee does not lay eggs, a new pollen mass is replaced every 2 days, and 50wt% sucrose water is fed at the same time. In order to facilitate the queen bee to lay eggs, the length, width and height of the egg-laying box are 12cm×8cm×8cm. At the same time, 2 bee eggs and 1 companion bee are provided to the new queen bee in the egg-laying period, such as Figure 2 Shown are bumblebees housed in an egg-laying box.

[0040] (3) Cultivation of mature bee colonies: After 14 days of cultivation, when the bee colony has developed into a colony of 20 worker bees, the entire bee colony is moved to a bee box for continued breeding, and the humidity of the environment is controlled to be 30% and the temperature is 22°C. At this time, the bee colony is fed with northern pollen, and a new pollen ball is added every day. At the same time, it is fed with 50wt% sucrose water. The colony is cultivated for 28 days until the bee colony develops into a colony of 80 worker bees. At this time, the bee colony is suitable for pollination of greenhouse crops; the length, width and height of the bee box are 28cm×28cm×24cm, such as Figure 3 Shown is a colony of bees housed in an adult bee box.

[0041] (4) Cultivation of virgin queens and male bumblebees: In order to continuously cultivate bee colonies, it is necessary to further cultivate virgin queens and male bumblebees. Specifically, select the bee colonies with a large number of worker bees and eggs from several mature bee colonies for further cultivation, maintain the same environmental temperature and feeding conditions, and cultivate for 28 days. The bee colony will produce new virgin queens and male bumblebees.

[0042] (5) Mating of virgin queens: 6-day-old virgin queens and 3-day-old male bumblebees were selected and placed in mating cages. 300 virgin queens and 600 male bumblebees were placed in each mating cage. The humidity of the mating room was controlled to be 30%, the temperature was 22°C, the light was strong, and the ventilation was high. A number of pollen balls and a portion of 50 wt% sucrose water were placed in each mating cage. The virgin queens and male bumblebees were kept together in the mating cages for 4 days. The mating cages were 1 m 3 Breathable mesh cage, such as Figure 4 Shown are a mating cage and bumblebees kept in the mating cage.

[0043] (6) Inducing queen bee hibernation: After mating is completed, place the mating cage in an environment of 16°C for 12 hours. After 12 hours, pick out the queen bee and place it in the hibernation room. Control the humidity of the hibernation room to 80% and the temperature to 1°C. The queen bee can survive in the hibernation room for six months. When it is necessary to cultivate a new bee colony, the hibernating queen bee can be revived. Figure 5 Shown is the hibernation chamber and the hibernating queen bee.

[0044] Example 2

[0045] The method for factory-based breeding of bumblebees in this embodiment comprises the following specific steps:

[0046] (1) Resuscitation of hibernating queen bees: The queen bees that hibernated for 3 months were taken out of the hibernation chamber, placed in a room temperature of 16°C and raised for 24 hours, and fed with pollen and 50 wt% sucrose water.

[0047] (2) Inducing queen bee to lay eggs: Each queen bee after resuscitation is placed in an egg-laying box separately, and the humidity of the environment is controlled to be 50% and the temperature is controlled to be 25°C. At this time, the queen bee is fed with a mixed powder of northern pollen, rapeseed powder and peach pollen in a weight ratio of 1.2:0.8:1.2. If the queen bee does not lay eggs, a new pollen mass is replaced every 3 days, and 50wt% sucrose water is fed at the same time. In order to facilitate the queen bee to lay eggs, the length, width and height of the egg-laying box are 12cm×8cm×8cm. At the same time, 2 bee eggs and 2 companion bees are provided to the new queen bee in the egg-laying period.

[0048] (3) Cultivation of mature bee colonies: After 21 days of cultivation, when the bee colony has developed into a colony of 25 worker bees, the entire bee colony is moved to a bee box for continued breeding, and the humidity of the environment is controlled to be 50% and the temperature is 24°C. At this time, the bee colony is fed with northern pollen, and a new pollen ball is added every day. At the same time, 50wt% sucrose water is fed. The colony is cultivated for 35 days until it develops into a colony of 100 worker bees. At this time, the bee colony is suitable for pollination of greenhouse crops; the length, width and height of the bee box are 28cm×28cm×24cm.

[0049] (4) Cultivation of virgin queens and male bumblebees: In order to continuously cultivate bee colonies, it is necessary to further cultivate virgin queens and male bumblebees. Specifically, select the bee colonies with a large number of worker bees and eggs from several mature bee colonies for further cultivation, maintain the same environmental temperature and feeding conditions, and cultivate for 35 days. The bee colony will produce new virgin queens and male bumblebees.

[0050] (5) Mating of virgin queens: 8-day-old virgin queens and 5-day-old male bumblebees were selected and placed in mating cages. 300 virgin queens and 600 male bumblebees were placed in each mating cage. The humidity of the mating room was controlled to be 50%, the temperature to be 23°C, the light intensity to be strong, and the ventilation to be strong. A number of pollen balls and a portion of 50 wt% sucrose water were placed in each mating cage. The virgin queens and male bumblebees were kept together in the mating cages for 6 days. The mating cages were 1 m 3 Breathable mesh cage.

[0051] (6) Inducing the queen bee to hibernate: After mating is completed, place the mating cage in an environment of 16°C for 24 hours. After 24 hours, pick out the queen bee and place it in the hibernation room. Control the humidity of the hibernation room to 90% and the temperature to 3°C. The queen bee can survive in the hibernation room for 6 months. When it is necessary to cultivate a new bee colony, the hibernating queen bee can be revived.

[0052] Example 3

[0053] The method for factory-based breeding of bumblebees in this embodiment comprises the following specific steps:

[0054] (1) Resuscitation of hibernating queen bees: The queen bees that hibernated for 3 months were taken out of the hibernation chamber, placed in a room temperature of 16°C and raised for 72 hours, and fed with pollen and 50 wt% sucrose water.

[0055] (2) Inducing queen bee to lay eggs: Each queen bee after resuscitation is placed separately in an egg-laying box, and the humidity of the environment is controlled to be 70% and the temperature is controlled to be 28°C. At this time, the queen bee is fed with a mixed powder of northern pollen, rapeseed powder and peach pollen in a weight ratio of 1.2:1.2:0.8. If the queen bee does not lay eggs, a new pollen mass is replaced every 5 days, and 50wt% sucrose water is fed at the same time. In order to facilitate the queen bee to lay eggs, the length, width and height of the egg-laying box are 12cm×8cm×8cm. At the same time, 3 bee eggs and 3 companion bees are provided to the new queen bee in the egg-laying period.

[0056] (3) Cultivation of mature bee colonies: After 28 days of cultivation, when the bee colony has developed into a colony of 30 worker bees, the entire bee colony is moved to a bee box for continued breeding, and the humidity of the environment is controlled to be 70% and the temperature is 26°C. At this time, the bee colony is fed with northern pollen, and a new pollen ball is added every day. At the same time, it is fed with 50wt% sucrose water. The colony is cultivated for 42 days until it develops into a colony of 120 worker bees. At this time, the bee colony is suitable for pollination of greenhouse crops; the length, width and height of the bee box are 28cm×28cm×24cm.

[0057] (4) Cultivation of virgin queens and male bumblebees: In order to continuously cultivate bee colonies, it is necessary to further cultivate virgin queens and male bumblebees. Specifically, select the bee colonies with a large number of worker bees and eggs from several mature bee colonies for further cultivation, maintain the same environmental temperature and feeding conditions, and cultivate for 42 days. The bee colony will produce new virgin queens and male bumblebees.

[0058] (5) Mating of virgin queens: 10-day-old virgin queens and 7-day-old male bumblebees were selected and placed in mating cages. 300 virgin queens and 600 male bumblebees were placed in each mating cage. The humidity of the mating room was controlled to 70%, the temperature to 24°C, strong light, and ventilation. Several pollen balls and a portion of 50 wt% sucrose water were placed in each mating cage. The virgin queens and male bumblebees were kept together in the mating cages for 8 days. The mating cages were 1 m 3 Breathable mesh cage.

[0059] (6) Inducing the queen bee to hibernate: After mating is completed, place the mating cage in an environment of 16°C for 72 hours. After 72 hours, pick out the queen bee and place it in the hibernation room. Control the humidity of the hibernation room to 95% and the temperature to 6°C. The queen bee can survive in the hibernation room for 6 months. When it is necessary to cultivate a new bee colony, the hibernating queen bee can be revived.

[0060] Comparative Example

[0061] The bumblebee breeding method of the comparative example comprises six steps: reviving a hibernating queen bee, inducing the queen bee to lay eggs, breeding a mature bee colony, breeding a virgin queen and male bumblebees, mating of the virgin queen, and inducing the queen bee to hibernate.

[0062] (1) Resuscitation of hibernating queen bees: The queen bees that hibernated for 3 months were taken out of the hibernation chamber, placed in a room temperature of 16°C and raised for 24 hours, and fed with pollen and 50 wt% sucrose water.

[0063] (2) Inducing queen bees to lay eggs: Each queen bee after resuscitation is placed in an egg-laying box, and the humidity of the environment is controlled to be 50% and the temperature is controlled to be 25°C. At this time, the queen bee is fed with northern pollen. If the queen bee does not lay eggs, a new pollen mass is replaced every 3 days, and 50wt% sucrose water is fed at the same time. In order to facilitate the queen bee to lay eggs, the length, width and height of the egg-laying box are 12cm×8cm×8cm.

[0064] (3) Cultivation of mature bee colonies: After 21 days of cultivation, when the bee colony has developed into a colony of 20 worker bees, the entire bee colony is moved to a bee box for continued breeding, and the humidity of the environment is controlled to be 50% and the temperature is 24°C. At this time, the bee colony is fed with northern pollen, and a new pollen ball is added every day. At the same time, it is fed with 50wt% sucrose water. The colony is cultivated for 35 days until it develops into a colony of 80 worker bees. At this time, the bee colony is suitable for pollination of greenhouse crops; the length, width and height of the bee box are 28cm×28cm×24cm.

[0065] (4) Cultivation of virgin queens and male bumblebees: In order to continuously cultivate bee colonies, it is necessary to further cultivate virgin queens and male bumblebees. Specifically, select the bee colonies with a large number of worker bees and eggs from several mature bee colonies for further cultivation, maintain the same environmental temperature and feeding conditions, and cultivate for 35 days. The bee colony will produce new virgin queens and male bumblebees.

[0066] (5) Mating of virgin queens: 8-day-old virgin queens and 5-day-old male bumblebees were selected and placed in mating cages. 300 virgin queens and 600 male bumblebees were placed in each mating cage. The humidity of the mating room was controlled to be 50%, the temperature to be 23°C, the light intensity to be strong, and the ventilation to be strong. A number of pollen balls and a portion of 50 wt% sucrose water were placed in each mating cage. The virgin queens and male bumblebees were kept together in the mating cages for 6 days. The mating cages were 1 m 3 Breathable mesh cage.

[0067] (6) Inducing the queen bee to hibernate: After mating is completed, place the mating cage in an environment of 16°C for 24 hours. After 24 hours, pick out the queen bee and place it in the hibernation room. Control the humidity of the hibernation room to 90% and the temperature to 3°C. The queen bee can survive in the hibernation room for six months. When it is necessary to cultivate a new bee colony, the hibernating queen bee can be revived.

[0068] Compared with the comparative example, when the method of Examples 1-3 is used to breed bumblebees, there are significant advantages in egg-laying rate, swarming rate and swarming time. The specific data are shown in the following table.

[0069]

[0070] Test Example 1 Egg laying rate test

[0071] 467 queen bees raised in egg-laying boxes were randomly divided into four groups. The first group of 120 queen bees was used as a blank control, that is, no companion bees or bee eggs were added to each egg-laying box; the second group of 122 queen bees had 1-3 companion bees added to each egg-laying box; the third group of 125 queen bees had 2-3 bee eggs added to each egg-laying box; the fourth group of 110 queen bees had 2-3 bee eggs and 1-3 companion bees added to each egg-laying box. While maintaining the same feeding environment and conditions for the four groups of queen bees, the egg-laying rate of the queen bees in the egg-laying box was tested, and the results are shown in the following table.

[0072]

[0073] The main difficulty of traditional bumblebee queen breeding methods is the low egg-laying rate of the queen. We found that providing the new queen with eggs and companion bees at the same time can significantly increase the egg-laying rate. Compared with the other three groups, when both eggs and companion bees were added, the egg-laying rate of the queen increased to nearly 90%, which was significantly higher than the other three groups (Chi-square test, P values ​​were all less than 0.05).

[0074] Test Example 2: Grouping Rate Test

[0075] In order to test the swarming rate of queen bees fed with different pollens after laying eggs, this test randomly divided 387 queen bees that had already laid eggs into four groups. The first group of 92 queen bees, as a single pollen control, were fed only with northern hundred pollen; the second group of 87 queen bees, as a single pollen control, were fed only with rapeseed powder; the third group of 89 queen bees, as a single pollen control, were fed only with peach pollen; the fourth group of 119 queen bees, were fed with mixed pollen, northern hundred pollen, rapeseed powder and peach pollen mixed in a weight ratio of 1:1:1; the fifth group of 80 queen bees, as other pollen controls, were fed only with camellia pollen. While keeping other conditions the same, the late swarming rate of queen bees that had already laid eggs was tested, and the results are shown in the following table.

[0076]

[0077] Another major difficulty in the traditional bumblebee queen breeding method is the low swarming rate of queens that have laid eggs. We found that feeding queens with a mixture of pollen from northern 100-flower pollen, rapeseed powder and peach pollen significantly increased the swarming rate, because each pollen has different nutrient contents. When the three pollens are mixed, the nutrient contents are more balanced and comprehensive. Compared with the first three groups of single pollen, the swarming rate of queens that have laid eggs increased to nearly 60% when fed with a mixture of the three pollens, which was significantly higher than the other three groups (Chi-square test, P values ​​were all less than 0.05). Compared with other types of single pollen (Camellia pollen), the mixed pollen we used also had a higher egg-laying rate (Chi-square test, P value less than 0.05). Compared with other types of mixed pollen, the combination of (northern 100-flower pollen + rapeseed powder + peach pollen) was also better than the combination of (Camellia pollen + rapeseed powder) or (Camellia pollen + rapeseed powder + peach pollen) in terms of swarming rate. The above tests show that the combination of (northern hundred pollen + rapeseed pollen + peach pollen) is the most suitable pollen combination for feeding bumblebees.

[0078] Test Example 3: Breeding cycle test

[0079] In order to test the effect of different environmental temperatures on the breeding cycle of bee colonies, 312 queen bees that have laid eggs were randomly divided into three groups. The first group had 99 queens, and the culture temperature was 18°C; the second group had 102 queens, and the culture temperature was 22°C; the third group had 111 queens, and the culture temperature was 26°C. While keeping other conditions the same, the breeding cycle of the bee colony was tested, and the results are shown in the following table.

[0080]

[0081] Another major difficulty of the traditional bumblebee queen breeding method is the long breeding cycle. When the ambient temperature is raised to 26°C, the breeding cycle will be significantly shortened. Compared with the other two groups, when the ambient temperature is 26°C, the breeding cycle is shortened by 8 days and 21 days respectively, and the breeding cycle is significantly shorter (independent sample t test, P values ​​are all less than 0.05).

[0082] The above description is a further detailed description of the present invention in combination with a specific preferred embodiment, and it cannot be determined that the specific implementation of the present invention is limited to the above description. For ordinary technicians in the technical field of the present invention, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should all fall within the scope of protection of the present invention.

Claims

1. A method for factory-based breeding of bumblebees, characterized in that: include: (1) Resuscitation of hibernating queen bees The queen bee which hibernates for 3-6 months is taken out from the hibernation chamber, placed in an environment with a room temperature of 14-18°C for 12-72 hours, and fed with pollen and 45-55wt% sucrose water; (2) Inducing the queen bee to lay eggs After the queen bee recovers, each queen bee is placed in an egg-laying box, and the humidity of the environment is controlled to be 30%-70% and the temperature is 23-28°C. At this time, the queen bee is fed with mixed pollen. If the queen bee does not lay eggs, a new pollen mass is replaced every 2-5 days, and 45-55wt% sucrose water is fed at the same time; (3) Cultivation of mature bee colonies When the bee colony develops into a colony strength of 20-30 worker bees, the entire bee colony is moved to a bee box for further breeding, and the humidity of the environment is controlled to be 30%-70% and the temperature is 22-26°C. At this time, the bee colony is fed with northern pollen, a new pollen ball is added every day, and 45-55wt% sucrose water is fed at the same time, and the bee colony is cultivated until the bee colony develops into a colony strength of 80-120 worker bees; (4) Breeding of virgin queens and male bumblebees Select a bee colony with a large number of worker bees and eggs from the mature bee colonies cultivated in step (3) and continue to cultivate it, maintain the same environmental temperature and feeding conditions, and cultivate it for 28-42 days. The bee colony will produce new virgin queens and male bumblebees; (5) Mating of the Virgin Queen Select 6-10 day old virgin queens and 3-7 day old male bumblebees and place them in mating cages, place 200-400 virgin queens and 400-800 male bumblebees in each mating cage, control the humidity of the mating room to 30%-70%, the temperature to 22-24° C., strong light, ventilation, place a number of pollen balls and a portion of 45-55wt% sucrose water in each mating cage, and keep the virgin queens and male bumblebees together in the mating cages for 4-8 days; (6) Inducing Queen Bee to Hibernate After mating is completed, place the mating cage in an environment of 14-18℃ and raise it for 12-72h. After 12-72h, pick out the queen bee and place it in the hibernation room. Control the humidity of the hibernation room to 80%-95% and the temperature to 1-6℃. When it is necessary to cultivate a new bee colony, revive the hibernating queen bee.

2. The method for industrial breeding of bumblebees as claimed in claim 1, characterized in that: In step (2), the mixed pollen is a mixed powder prepared by mixing northern gentian pollen, rapeseed pollen and peach pollen in a weight ratio of (0.8-1.2):(0.8-1.2):(0.8-1.2).

3. The method for industrialized breeding of bumblebees as claimed in claim 1, characterized in that: In step (2), 2-3 bee eggs and 1-3 companion bees are added to each egg-laying box.

4. The method for industrial breeding of bumblebees as claimed in claim 1, characterized in that: The length, width and height of the egg-laying box are 12cm×8cm×8cm.

5. The method for industrial breeding of bumblebees as claimed in claim 1, characterized in that: The length, width and height of the adult bee box are 28cm×28cm×24cm.

6. The method for industrial breeding of bumblebees as claimed in claim 1, characterized in that: The mating cage is 1m 3 Breathable mesh cage.

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