Functional peptide screening method, device, electronic device and storage medium

Abstract

The present invention provides a method, device, electronic device and storage medium for screening functional peptides, and relates to the field of biochemical technology, wherein the method comprises: based on a first color set, marking the sequence of functional peptides in the amino acid sequence of a target protein to obtain a first marking sequence; based on a second color set, marking the sequence of an enzyme-cut peptide segment to obtain a second marking sequence; overlapping the first marking sequence and the second marking sequence to obtain an overlapping sequence, and identifying the overlapping colors in the overlapping sequence to determine target peptide segments with multiple functions in the target protein and the enzyme-cutting method of the target peptide segment. Based on determining the overlapping sequence and identifying the overlapping colors in the obtained overlapping sequence, it is possible to quickly locate the target peptide segments with multiple functions in the target protein and the enzyme-cutting method of the target peptide segment, thereby improving the screening efficiency of multiple functional peptide segments and realizing a rapid screening process for multiple functional peptide segments in the target protein.

Description

Technical Field

[0001] The present invention relates to the field of biochemical technology, and in particular to a method, device, electronic equipment and storage medium for screening functional peptides. Background Art

[0002] After determining the protein that needs to be studied, the protein will be hydrolyzed based on different enzymatic conditions to obtain different enzymatic peptides. The obtained enzymatic peptides will be compared with the functional peptides in the database one by one to analyze the enzymatic peptides one by one, determine the function of each enzymatic peptide, and thus screen out peptides with multiple functions.

[0003] The existing methods are based on comparing the functional peptides in the database one by one and determining the functions of the enzymatic peptides one by one. This method is inefficient and cannot quickly realize the rapid screening process of multiple functional peptides. Summary of the invention

[0004] The present invention provides a method, device, electronic equipment and storage medium for screening functional peptides, which are used to improve the screening efficiency of multiple functional peptide segments.

[0005] The present invention provides a method for screening functional peptides, comprising the following steps:

[0006] Determine the functional peptides in the target protein, and mark the sequence of the functional peptides in the amino acid sequence of the target protein based on the first color set to obtain a first marked sequence, wherein the color categories in the first color set correspond one-to-one to the functional categories of the functional peptides;

[0007] Determine the enzyme-cleaved peptides in the target protein, and mark the sequences of the enzyme-cleaved peptides in the amino acid sequence based on a second color set to obtain a second marked sequence, wherein the enzyme-cleaved peptides are peptides obtained by enzymatically hydrolyzing the target protein based on different enzyme cleavage methods, the color categories in the second color set correspond one-to-one to each enzyme cleavage method, and the colors in the second color set are different from the colors in the first color set;

[0008] The first marker sequence and the second marker sequence are overlapped to obtain an overlapped sequence, and based on the overlapped colors in the overlapped sequence, target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides are determined.

[0009] According to a method for screening functional peptides provided by the present invention, after determining the target peptide segments with multiple functions in the target protein, the method further comprises:

[0010] Based on analyzing the colors contained in the target peptide segment, the corresponding relationship between the enzyme cleavage mode in the target peptide segment and the functional peptide in the target peptide segment is determined.

[0011] According to a method for screening functional peptides provided by the present invention, the sequence of the enzyme-cleaved peptide segment in the amino acid sequence is marked based on the second color set, further comprising:

[0012] Based on the number of peptides obtained by different enzyme cleavage methods, determine the depth of the annotation color corresponding to different enzyme cleavage methods;

[0013] Based on the second color set and the depth of the marked colors corresponding to different enzyme cleavage methods, the sequence of the enzyme-cleaved peptide segment in the amino acid sequence is marked.

[0014] According to a method for screening functional peptides provided by the present invention, determining the enzymatic cleavage mode of the target peptide segment comprises:

[0015] The enzymatic cleavage mode of the target peptide segment is determined based on the depth of the color of the target peptide segment in the overlapping sequence.

[0016] According to a method for screening functional peptides provided by the present invention, the step of determining the functional peptides in the target protein comprises:

[0017] Based on the amino acid sequence of the target protein, the functional peptides in the target protein are matched with the functional peptide sequences in a pre-constructed functional peptide database to determine the functional peptides in the target protein, wherein the functional peptide database is constructed based on a variety of different functional peptide sequences.

[0018] According to a method for screening functional peptides provided by the present invention, after determining the target peptide segments with multiple functions in the target protein and the enzyme cleavage method of the target peptide segments, the method further comprises:

[0019] Based on identifying the positions of the overlapping colors in the overlapping sequence, the position information of the target peptide segment in the amino acid sequence of the target protein is determined.

[0020] The present invention also provides a functional peptide screening device, comprising the following modules:

[0021] A functional peptide labeling module, used to determine the functional peptides in the target protein, and label the sequence of the functional peptides in the amino acid sequence of the target protein based on a first color set to obtain a first labeling sequence, wherein the color categories in the first color set correspond one-to-one to the functional categories of the functional peptides;

[0022] an enzyme-cleaved peptide segment annotation module, for determining the enzyme-cleaved peptide segments in the target protein, and marking the sequences of the enzyme-cleaved peptide segments in the amino acid sequence based on a second color set to obtain a second labeled sequence, wherein the enzyme-cleaved peptide segments are peptide segments obtained by enzymatically hydrolyzing the target protein based on different enzyme-cleavage methods, the color categories in the second color set correspond one-to-one to each enzyme-cleavage method, and the colors in the second color set are different from the colors in the first color set;

[0023] The screening module is used to overlap the first marker sequence and the second marker sequence to obtain an overlapping sequence, and determine the target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides based on the overlapping colors in the overlapping sequence.

[0024] The present invention also provides an electronic device, comprising a memory, a processor, and a computer program stored in the memory and running on the processor, wherein when the processor executes the computer program, the screening method for functional peptides described above is implemented.

[0025] The present invention also provides a non-transitory computer-readable storage medium having a computer program stored thereon, and when the computer program is executed by a processor, the method for screening functional peptides as described in any one of the above is implemented.

[0026] The present invention also provides a computer program product, comprising a computer program, wherein when the computer program is executed by a processor, the method for screening any of the functional peptides described above is implemented.

[0027] The screening method, device, electronic device and storage medium of functional peptides provided by the present invention mark the functional peptides in the amino acid sequence of the target protein by a first color set to obtain a first marking sequence, and mark the sequence of the enzyme cleavage peptide segment corresponding to the enzyme cleavage method in the amino acid sequence of the target protein based on the second color set to obtain a second marking sequence. Based on the overlap of the first marking sequence and the second marking sequence, the overlapping colors can be intuitively viewed from the obtained overlapping sequence, and the overlapping colors in the obtained overlapping sequence can be identified, so that the target peptide segments with multiple functions in the target protein and the enzyme cleavage method of the target peptide segment can be quickly located, thereby improving the screening efficiency of multiple functional peptide segments and realizing a rapid screening process for multiple functional peptide segments in the target protein. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0029] Figure 1 It is a schematic diagram of the process of the screening method of functional peptides provided by the present invention.

[0030] Figure 2 This is a schematic diagram of the first marker sequence provided by the present invention.

[0031] Figure 3 It is a schematic diagram of the second marker sequence provided by the present invention.

[0032] Figure 4 It is a schematic diagram of the overlap sequence provided by the present invention.

[0033] Figure 5 It is a schematic diagram of the structure of the screening device for functional peptides provided by the present invention.

[0034] Figure 6 It is a structural schematic diagram of the electronic device provided by the present invention. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] Figure 1 is a schematic diagram of the process of screening the functional peptides provided by the present invention, such as Figure 1 As shown, the method includes the following:

[0037] Step 110, determining the functional peptides in the target protein, and marking the sequences of the functional peptides in the amino acid sequence of the target protein based on a first color set to obtain a first marked sequence, wherein the color categories in the first color set correspond one-to-one to the functional categories of the functional peptides;

[0038] Step 120, determining the enzyme-cleaved peptides in the target protein, and marking the sequences of the enzyme-cleaved peptides in the amino acid sequence based on a second color set to obtain a second marked sequence, wherein the enzyme-cleaved peptides are peptides obtained by enzymatically digesting the target protein based on different enzyme cleavage methods, and the color categories in the second color set correspond to the enzyme cleavage methods one by one, and the colors in the second color set are different from the colors in the first color set;

[0039] Step 130, overlapping the first marker sequence and the second marker sequence to obtain an overlapping sequence, and determining target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides based on the overlapping colors in the overlapping sequence.

[0040] The execution subject of the screening method for functional peptides provided by the present invention can be an electronic device, a component in an electronic device, an integrated circuit, or a chip. The electronic device can be a mobile electronic device or a non-mobile electronic device. Exemplarily, the mobile electronic device can be a mobile phone, a tablet computer, a laptop computer, a PDA, an ultra-mobile personal computer (UMPC), a netbook, or a personal digital assistant (PDA), etc., and the non-mobile electronic device can be a server, a network attached storage (NAS), or a personal computer (PC), etc., which is not specifically limited by the present invention.

[0041] The following takes the method for screening functional peptides provided by the present invention executed by a computer as an example to describe the technical solution of the present invention in detail.

[0042] In step 110, the functional peptides in the target protein are first determined.

[0043] Functional peptides are peptide substances that have important regulatory functions in organisms, also known as bioactive peptides.

[0044] Functional peptides may include: antioxidant peptides, immunomodulatory peptides, antimicrobial peptides, blood pressure lowering peptides, etc.

[0045] Among them, antioxidant peptides have antioxidant effects and can protect cells from oxidative stress damage. They play antioxidant roles by scavenging free radicals or inhibiting oxidative reactions; immunomodulatory peptides are used to play an important regulatory role in the body and regulate the function of the immune system; and antihypertensive peptides are used to regulate blood pressure.

[0046] The functional peptides of the target protein can be determined based on a pre-constructed functional peptide database. The functional peptide database is a database constructed by summarizing the functional peptides and sequences of the functional peptides in public texts.

[0047] By determining the amino acid sequence of the target protein and matching the determined amino acid sequence of the target protein with the sequences of each functional peptide contained in the functional peptide database, it is possible to determine which functional peptides are specifically contained in the target protein and the specific composition of the amino acid peptide segments of these functional peptides.

[0048] After the functional peptides in the target protein are determined, the sequence of the functional peptides in the amino acid sequence of the target protein is marked based on the first color set. The sequence of the functional peptide refers to the amino acid sequence of the functional peptide.

[0049] The color categories in the first color set correspond to the functional categories of the functional peptides one by one. Specifically, the first color set includes multiple color categories, such as red, yellow, and green. Alternatively, based on the RGB model, the color categories are digitally represented.

[0050] Each color category in the first color set corresponds to a functional peptide of a functional category. For example, red corresponds to antioxidant peptides, green corresponds to immunomodulatory peptides, and yellow corresponds to blood pressure lowering peptides.

[0051] The sequences of the functional peptides in the amino acid sequence of the target protein are marked. Specifically, the sequences corresponding to all antioxidant peptides in the amino acid sequence of the target protein can be marked in red, the sequences corresponding to all immunomodulatory peptides in the amino acid sequence of the target protein can be marked in green, and the sequences corresponding to all blood pressure lowering peptides in the amino acid sequence of the target protein can be marked in yellow. The marking process of the sequences of all functional peptides in the amino acid sequence of the target protein is completed to obtain a first marked sequence.

[0052] Taking the amino acid sequence of β-casein, which is composed of 224 amino acids, the specific antioxidant peptide sequences included are: "fpkypvepf", "swmhqphqplppt", the immunomodulatory peptide sequences included are: "yqepvlgpvr", "ypfpgpi", and the antihypertensive peptide sequences included are: "ypvepf", "ypfpgp". The sequences corresponding to all immunomodulatory peptides based on β-casein are marked in green, the sequences corresponding to all antihypertensive peptides are marked in yellow, and the sequences corresponding to all antioxidant peptides are marked in red. The first marker sequence can be obtained as follows Figure 2 The first marker sequence provided by the present invention is shown in a schematic diagram.

[0053] It is understandable that after obtaining the first marker sequence, the sequence containing multiple functional peptides can be quickly determined based on the identification of the overlapping colors in the first marker sequence. Figure 2 The sequence "ypfpgpi" overlapped in yellow and green, and the sequence "fpkypvepf" overlapped in red and yellow, both contain functional peptides with two functions.

[0054] Step 120, determining the enzyme-cleaved peptide segments in the target protein.

[0055] Enzyme-digested peptides are peptides obtained by enzymatic hydrolysis of the target protein based on different enzymatic hydrolysis methods. The enzymatic hydrolysis methods include different enzyme types and concentrations, different temperatures, different enzymatic hydrolysis times, etc.

[0056] It is understandable that different enzymes and different enzymatic hydrolysis conditions can be used to act on the target protein to obtain different enzyme-digested fragments.

[0057] After the target protein is enzymatically digested by different enzyme cleavage methods, the enzyme cleavage peptides corresponding to the different enzyme cleavage methods can be determined.

[0058] Based on the second color set, the sequence of the enzyme-cleaved peptide segment in the amino acid sequence is marked to obtain a second marked sequence.

[0059] Specifically, the color categories in the second color set correspond to the enzyme cutting methods one by one. The second color set includes multiple color categories that are different from the color categories in the first color set, such as brown, black, blue, etc. Alternatively, based on the RGB model, the color categories are digitally represented.

[0060] Each color category in the second color set corresponds to a peptide segment cleaved by a certain enzyme cleavage method. For example, brown corresponds to a peptide segment cleaved by protease A at 20 degrees Celsius for 3 hours, and blue corresponds to a peptide segment cleaved by protease B at 20 degrees Celsius for 4 hours.

[0061] The sequences of the enzymatic peptides in the amino acid sequence of the target protein are marked. Specifically, the sequences of all the enzymatic peptides obtained by enzymatic cleavage of protease A in the amino acid sequence of the target protein at 20 degrees Celsius for 3 hours can be marked as brown, and the sequences of all the enzymatic peptides obtained by enzymatic cleavage of protease B in the amino acid sequence of the target protein at 20 degrees Celsius for 4 hours can be marked as blue. The marking process of the corresponding sequences of the enzymatic peptides of all enzymatic cleavage methods in the amino acid sequence of the target protein is completed to obtain the second marked sequence.

[0062] Taking the amino acid sequence of β-casein, which consists of 224 amino acids, the enzymatic cleavage peptides obtained by protease A at 20 degrees Celsius for 3 hours are: "eesitr" and "ypfpgp". The enzymatic cleavage peptides obtained by this enzymatic cleavage method are marked in brown, and the obtained second sequence can be as follows Figure 3 The second marker sequence provided by the present invention is shown in a schematic diagram.

[0063] In step 130, after obtaining the first marking sequence and the second marking sequence, the first marking sequence and the second marking sequence are overlapped to obtain an overlapped sequence.

[0064] It can be understood that the first marker sequence and the second marker sequence are sequences obtained by marking the amino acid sequence of the target protein with different colors. The amino acid arrangement contained in the sequence is the same, but it contains different color marks, so the first marker sequence and the second marker sequence can be overlapped to obtain an overlapping sequence.

[0065] After obtaining the overlapping sequences, color analysis can be performed on the overlapping sequences to quickly locate target peptides with multiple functions in the target protein and the enzyme cleavage mode of the target peptides.

[0066] It can be understood that the new color obtained after the overlap of different colors is different from the colors in the first color set and the second color set. A new color can be obtained, which does not belong to the first color set and the second color set. It can be quickly identified from the overlapping sequence based on computer vision to achieve a rapid positioning process of the target peptide.

[0067] Based on the RGB model, the overlapping colors can be analyzed to extract multiple single colors before mixing, thereby analyzing the multiple functions contained in the target peptide and the enzyme cleavage method of the target peptide.

[0068] like Figure 4 The schematic diagram of the overlapping sequence provided by the present invention is shown. The polypeptide with the sequence "ypfpgpi" has three overlaps. Its "ypfpgp" sequence part is predicted to be a blood pressure lowering functional peptide (yellow background representation), its "ypfpgpi" sequence is an immunomodulatory functional peptide (green representation), and "ypfpgpi" is also a polypeptide obtained by enzyme cleavage with protease A (brown representation).

[0069] In summary, researchers can easily and quickly screen out the active peptide combination with "ypfpgpi" as the dominant product based on overlapping colors through enzymatic hydrolysis by protease A under specified conditions. This enzymatic hydrolysis product has a strong immunomodulatory function. At the same time, the dominant product "ypfpgpi" contains "ypfpgp" which is predicted to have antihypertensive activity. In future studies, we can focus on observing the content of this sequence polypeptide in the enzymatic hydrolysis product and whether the enzymatic hydrolysis product also has antihypertensive activity.

[0070] It can be understood that the specific advantages of the screening process based on coincident colors include:

[0071] First, it can realize the rapid screening of peptides with multiple functions. Functional peptides with different functions are represented by different colors. When the peptides with the same amino acid sequence have more than one function, the colors representing two or more functions overlap, and a new third color will appear. By identifying the third color, functional peptide sequences with multiple functions can be quickly screened out;

[0072] Second, rapid screening of enzyme preparations can be achieved. The cleavage fragments of different enzymes are also characterized by different colors. When the cleavage fragments of different enzymes overlap with the functional peptide sequence, another overlapping color is displayed. For example, the cleavage fragments of enzyme A overlap with the antioxidant active peptide 3 times, while the cleavage fragments of enzyme A overlap with the antihypertensive peptide 10 times. It can be judged that enzyme A can be preferentially selected to prepare enzymatic hydrolysates rich in antihypertensive peptides, which can help to preferentially select this enzyme as the main choice for preparing specific functional peptides.

[0073] Third, it helps determine the main function of the enzymatic product. After any enzyme hydrolyzes the target protein, the composition and relative abundance of active peptides in the hydrolysate can be known through peptidomics analysis. By overlapping with functional peptides, the overlapping colors can reflect the main function of the enzymatic product.

[0074] The screening method of functional peptides provided by the present invention marks the functional peptides in the amino acid sequence of the target protein by a first color set to obtain a first marking sequence, and marks the sequence of the enzyme cleavage peptide corresponding to the enzyme cleavage method in the amino acid sequence of the target protein based on the second color set to obtain a second marking sequence. Based on the overlap of the first marking sequence and the second marking sequence, the overlapping colors can be intuitively viewed from the obtained overlapping sequence, and the overlapping colors in the obtained overlapping sequence can be identified, so that the target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptide can be quickly located, thereby improving the screening efficiency of multiple functional peptides and realizing a rapid screening process for multiple functional peptides in the target protein.

[0075] In one embodiment, after determining the target peptide segment with multiple functions in the target protein, the method further includes: determining the correspondence between the enzyme cleavage mode in the target peptide segment and the functional peptide in the target peptide segment based on analyzing the color contained in the target peptide segment.

[0076] Specifically, the new color obtained after the different colors overlap can be quickly identified from the overlapping sequence based on computer vision to identify the overlapping colors in the overlapping sequence.

[0077] After obtaining the overlapping colors, reverse analysis is performed on the overlapping colors to extract multiple single colors before mixing. This allows the analysis of multiple functions contained in the target peptide and the enzyme cleavage method of the target peptide. The functional peptides and enzyme cleavage methods corresponding to the target peptide are associated to obtain the corresponding relationship between the enzyme cleavage method in the target peptide and the functional peptide in the target peptide.

[0078] It is understandable that researchers can implement the analysis process of the target protein based on the determined correspondence, and can also implement the rapid acquisition process of the functional peptide corresponding to the target peptide based on the correspondence and the enzyme cleavage method in the correspondence.

[0079] In one embodiment, based on the second color set, the sequence of the enzyme-cut peptide segment in the amino acid sequence is marked, further comprising: determining the depth of the marking color corresponding to the different enzyme cutting methods based on the number of peptide segments obtained by different enzyme cutting methods; based on the second color set and the depth of the marking color corresponding to the different enzyme cutting methods, the sequence of the enzyme-cut peptide segment in the amino acid sequence is marked.

[0080] Based on the depth of the color of the target peptide in the overlapping sequence, the enzymatic cleavage mode of the target peptide can be determined.

[0081] Specifically, the color depth of each color category in the second color set can be graded. For example, brown in the second color set corresponds to all enzyme-digested peptides obtained by enzyme digestion of protease A at 20 degrees Celsius for 3 hours. The brown in the second color set is graded, and the levels can be set to light brown and dark brown.

[0082] In the process of marking based on the second color set, the marking process of different brown levels is performed according to the number of all cleavage peptides obtained by the enzymatic cleavage of protease A in an environment of 20 degrees Celsius for 3 hours. For example, when the number of all cleavage peptides obtained is greater than a preset number threshold, dark brown is used for marking, and when it is less than or equal to the preset number threshold, light brown is used for marking. The selection of a specific color level can be based on the number of one of the peptides to be studied among all the cleavage peptides obtained by the enzymatic cleavage of protease A in an environment of 20 degrees Celsius for 3 hours.

[0083] After color marking, the overlapped sequences obtained later will be different. The depth of the overlapped color that can be obtained can be used to determine the number of peptides obtained by enzyme cleavage.

[0084] According to the quantity requirement of the target peptides by the scientific research staff, the enzymatic cleavage method of the target peptides can be determined based on the depth of the color of the target peptides in the overlapping sequence, so as to achieve rapid acquisition of the target peptides of the quantity corresponding to the requirement.

[0085] For example, the peptides obtained by enzymatic digestion are represented by light brown to dark brown. When the peptides obtained by enzymatic digestion overlap with the functional peptides, the dark-colored peptide sequence is the functional peptide that can be obtained by this enzymatic digestion condition, and the number is large. In practice, it is assumed that the functional peptide is a functional peptide related to immune activity. In order to enhance the function, it is more likely to obtain an enzymatic digestion product with high yield of immune active peptides using this enzymatic digestion condition such as protease A. Researchers can prefer protease A to produce enzymatic digestion products with high immune activity. It is also possible to separate and extract active peptides with high immune activity at a higher concentration from this enzymatic digestion product.

[0086] In one embodiment, determining the functional peptides in the target protein includes: matching the amino acid sequence of the target protein with the functional peptide sequences in a pre-constructed functional peptide database to determine the functional peptides in the target protein, wherein the functional peptide database is constructed based on multiple different functional peptide sequences.

[0087] A functional peptide database is pre-built, which can integrate functional peptide sequences from multiple public databases. Functional peptides can be classified according to their biological functions, such as antimicrobial peptides, immunomodulatory peptides, blood pressure lowering peptides, antioxidant peptides, etc. The database can also provide functional descriptions and detailed information for different categories of peptides.

[0088] Optionally, the amino acid sequence of the target protein can be compared with the known functional peptide sequences in the functional peptide database using a sequence alignment algorithm (such as BLAST, Smith-Waterman, Needleman-Wunsch, etc.) to find similar peptides. If the peptide of the target protein has a high sequence similarity with a peptide in a functional peptide database, it can be determined that the peptide may have the same biological activity.

[0089] It can be understood that by matching the amino acid sequence of the target protein with the functional peptide database, the functional peptides contained in the target protein can be quickly identified.

[0090] In one embodiment, after determining the target peptides with multiple functions in the target protein and the enzymatic cleavage method of the target peptides, it also includes: based on identifying the positions of overlapping colors in the overlapping sequences, determining the position information of the target peptides in the amino acid sequence of the target protein.

[0091] The use of overlapping colors can not only achieve rapid identification of the target peptide, but also further locate the position of the target peptide.

[0092] By identifying the overlapping colors and combining them with the numbering of the amino acid sequence of the target protein, the site of the target peptide can be directly mapped. For example, if the overlapping colors in the overlapping sequence show that a functional peptide is highly matched with a certain region in the target protein (such as positions 20-40), then it can be determined that the peptide is located between positions 20-40 of the target protein.

[0093] It is understandable that after using the overlap color analysis, researchers can also analyze the distribution of functional peptides in the target protein. For example, whether the target peptides are clustered in a specific area, whether they are related to the active site, binding site, etc. of the protein. Such analysis helps to reveal the potential biological function or mechanism of action of the target protein.

[0094] By accurately locating target peptides, especially those with biological activity, important clues can be provided for drug design. For example, in the development of anticancer, antiviral, antibacterial and other functional drugs, the region where certain functional peptides are located can be identified and located, allowing researchers to design targeted drugs for these peptides or the domains where the peptides are located. By accurately locating the target peptides, researchers can ensure the target selectivity in the experiment.

[0095] The functional peptide screening device provided by the present invention is described below. The functional peptide screening device described below and the functional peptide screening method described above can be referenced to each other.

[0096] like Figure 5 As shown, the device comprises:

[0097] The functional peptide labeling module 510 is used to determine the functional peptides in the target protein, and label the sequence of the functional peptides in the amino acid sequence of the target protein based on the first color set to obtain a first labeling sequence, wherein the color categories in the first color set correspond one-to-one to the functional categories of the functional peptides;

[0098] The enzyme cleavage peptide segment labeling module 520 is used to determine the enzyme cleavage peptide segments in the target protein, and mark the sequences of the enzyme cleavage peptide segments in the amino acid sequence based on the second color set to obtain a second labeled sequence, wherein the enzyme cleavage peptide segments are peptide segments obtained by enzymatically hydrolyzing the target protein based on different enzyme cleavage methods, and the color categories in the second color set correspond to each enzyme cleavage method one by one, and the colors in the second color set are different from the colors in the first color set;

[0099] The screening module 530 is used to overlap the first marker sequence and the second marker sequence to obtain an overlapping sequence, and determine the target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides based on the overlapping colors in the overlapping sequence.

[0100] The screening device for functional peptides provided by the present invention marks the functional peptides in the amino acid sequence of the target protein by a first color set to obtain a first marking sequence, and marks the sequence of the enzyme cleavage peptide segment corresponding to the enzyme cleavage method in the amino acid sequence of the target protein based on the second color set to obtain a second marking sequence. Based on the overlap of the first marking sequence and the second marking sequence, the overlapping colors can be intuitively viewed from the obtained overlapping sequence, and the overlapping colors in the obtained overlapping sequence can be identified, so that the target peptide segments with multiple functions in the target protein and the enzyme cleavage method of the target peptide segment can be quickly located, thereby improving the screening efficiency of multiple functional peptide segments and realizing a rapid screening process for multiple functional peptide segments in the target protein.

[0101] In one embodiment, the screening module 530 is specifically used to:

[0102] After determining the target peptides with multiple functions in the target protein, the method further includes:

[0103] Based on analyzing the colors contained in the target peptide segment, the corresponding relationship between the enzyme cleavage mode in the target peptide segment and the functional peptide in the target peptide segment is determined.

[0104] In one embodiment, the enzyme-cleaved peptide segment annotation module 520 is specifically used for:

[0105] Based on the second color set, marking the sequence of the enzyme-cleaved peptide segment in the amino acid sequence further includes:

[0106] Based on the number of peptides obtained by different enzyme cleavage methods, determine the depth of the annotation color corresponding to different enzyme cleavage methods;

[0107] Based on the second color set and the depth of the marked colors corresponding to different enzyme cleavage methods, the sequence of the enzyme-cleaved peptide segment in the amino acid sequence is marked.

[0108] In one embodiment, the enzyme-cleaved peptide segment annotation module 520 is further specifically used for:

[0109] Determine the enzymatic cleavage mode of the target peptide, including:

[0110] The enzymatic cleavage mode of the target peptide segment is determined based on the depth of the color of the target peptide segment in the overlapping sequence.

[0111] In one embodiment, the functional peptide annotation module 510 is specifically used for:

[0112] The method of determining the functional peptides in the target protein comprises:

[0113] Based on the amino acid sequence of the target protein, the functional peptides in the target protein are matched with the functional peptide sequences in a pre-constructed functional peptide database to determine the functional peptides in the target protein, wherein the functional peptide database is constructed based on a variety of different functional peptide sequences.

[0114] In one embodiment, the screening module 530 is further specifically configured to:

[0115] After determining the target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides, the method further includes:

[0116] Based on identifying the positions of the overlapping colors in the overlapping sequence, the position information of the target peptide segment in the amino acid sequence of the target protein is determined.

[0117] Figure 6 An example of a physical structure diagram of an electronic device is shown in FIG. Figure 6 As shown, the electronic device may include: a processor 610, a communications interface 620, a memory 630 and a communications bus 640, wherein the processor 610, the communications interface 620 and the memory 630 communicate with each other through the communications bus 640. The processor 610 may call the logic instructions in the memory 630 to execute a method for screening functional peptides, the method comprising: determining a functional peptide in a target protein, and marking a sequence of the functional peptide in the amino acid sequence of the target protein based on a first color set to obtain a first marked sequence, wherein the color categories in the first color set correspond one-to-one to the functional categories of the functional peptides;

[0118] Determine the enzyme-cleaved peptides in the target protein, and mark the sequences of the enzyme-cleaved peptides in the amino acid sequence based on a second color set to obtain a second marked sequence, wherein the enzyme-cleaved peptides are peptides obtained by enzymatically hydrolyzing the target protein based on different enzyme cleavage methods, the color categories in the second color set correspond one-to-one to each enzyme cleavage method, and the colors in the second color set are different from the colors in the first color set;

[0119] The first marker sequence and the second marker sequence are overlapped to obtain an overlapped sequence, and based on the overlapped colors in the overlapped sequence, target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides are determined.

[0120] In addition, the logic instructions in the above-mentioned memory 630 can be implemented in the form of a software functional unit and can be stored in a computer-readable storage medium when it is sold or used as an independent product. Based on such an understanding, the technical solution of the present invention, in essence, or the part that contributes to the prior art or the part of the technical solution, can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including a number of instructions for a computer device (which can be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the method described in each embodiment of the present invention. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), disk or optical disk, etc. Various media that can store program codes.

[0121] On the other hand, the present invention also provides a computer program product, the computer program product includes a computer program, the computer program can be stored in a non-transitory computer-readable storage medium, when the computer program is executed by a processor, the computer can execute the functional peptide screening method provided by the above methods, the method comprising: determining the functional peptide in the target protein, and based on a first color set, marking the sequence of the functional peptide in the amino acid sequence of the target protein to obtain a first marked sequence, the color categories in the first color set corresponding to the functional categories of the functional peptides one by one;

[0122] Determine the enzyme-cleaved peptides in the target protein, and mark the sequences of the enzyme-cleaved peptides in the amino acid sequence based on a second color set to obtain a second marked sequence, wherein the enzyme-cleaved peptides are peptides obtained by enzymatically hydrolyzing the target protein based on different enzyme cleavage methods, the color categories in the second color set correspond one-to-one to each enzyme cleavage method, and the colors in the second color set are different from the colors in the first color set;

[0123] The first marker sequence and the second marker sequence are overlapped to obtain an overlapped sequence, and based on the overlapped colors in the overlapped sequence, target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides are determined.

[0124] In another aspect, the present invention further provides a non-transitory computer-readable storage medium having a computer program stored thereon, which is implemented when the computer program is executed by a processor to perform the screening method for functional peptides provided by the above methods, the method comprising: determining a functional peptide in a target protein, and marking a sequence of the functional peptide in the amino acid sequence of the target protein based on a first color set to obtain a first marked sequence, wherein the color categories in the first color set correspond one-to-one to the functional categories of the functional peptides;

[0125] Determine the enzyme-cleaved peptides in the target protein, and mark the sequences of the enzyme-cleaved peptides in the amino acid sequence based on a second color set to obtain a second marked sequence, wherein the enzyme-cleaved peptides are peptides obtained by enzymatically hydrolyzing the target protein based on different enzyme cleavage methods, the color categories in the second color set correspond one-to-one to each enzyme cleavage method, and the colors in the second color set are different from the colors in the first color set;

[0126] The first marker sequence and the second marker sequence are overlapped to obtain an overlapped sequence, and based on the overlapped colors in the overlapped sequence, target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides are determined.

[0127] The device embodiments described above are merely illustrative, wherein the units described as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units, that is, they may be located in one place, or they may be distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the scheme of this embodiment. Ordinary technicians in this field can understand and implement it without paying creative labor.

[0128] Through the description of the above implementation methods, those skilled in the art can clearly understand that each implementation method can be implemented by means of software plus a necessary general hardware platform, and of course, can also be implemented by hardware. Based on this understanding, the above technical solution is essentially or the part that contributes to the prior art can be embodied in the form of a software product, and the computer software product can be stored in a computer-readable storage medium, such as ROM / RAM, a disk, an optical disk, etc., including a number of instructions for a computer device (which can be a personal computer, a server, or a network device, etc.) to execute the methods described in each embodiment or some parts of the embodiments.

[0129] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for screening functional peptides, characterized in that: include: Determine the functional peptides in the target protein, and mark the sequence of the functional peptides in the amino acid sequence of the target protein based on the first color set to obtain a first marked sequence, wherein the color categories in the first color set correspond one-to-one to the functional categories of the functional peptides; Determine the enzyme-cleaved peptides in the target protein, and mark the sequences of the enzyme-cleaved peptides in the amino acid sequence based on a second color set to obtain a second marked sequence, wherein the enzyme-cleaved peptides are peptides obtained by enzymatically hydrolyzing the target protein based on different enzyme cleavage methods, the color categories in the second color set correspond one-to-one to each enzyme cleavage method, and the colors in the second color set are different from the colors in the first color set; The first marker sequence and the second marker sequence are overlapped to obtain an overlapped sequence, and based on the overlapped colors in the overlapped sequence, target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides are determined.

2. The method for screening functional peptides according to claim 1, characterized in that: After determining the target peptide segments with multiple functions in the target protein, the method further includes: Based on analyzing the colors contained in the target peptide segment, the corresponding relationship between the enzyme cleavage mode in the target peptide segment and the functional peptide in the target peptide segment is determined.

3. The method for screening functional peptides according to claim 1, characterized in that: The step of marking the sequence of the enzyme-cleaved peptide segment in the amino acid sequence based on the second color set further comprises: Based on the number of peptides obtained by different enzyme cleavage methods, determine the depth of the annotation color corresponding to different enzyme cleavage methods; Based on the second color set and the depth of the marked colors corresponding to different enzyme cleavage methods, the sequence of the enzyme-cleaved peptide segment in the amino acid sequence is marked.

4. The method for screening functional peptides according to claim 3, characterized in that: Determine the enzymatic cleavage mode of the target peptide, including: The enzymatic cleavage mode of the target peptide segment is determined based on the depth of the color of the target peptide segment in the overlapping sequence.

5. The method for screening functional peptides according to claim 1, characterized in that: The method of determining the functional peptides in the target protein comprises: Based on the amino acid sequence of the target protein, the functional peptides in the target protein are matched with the functional peptide sequences in a pre-constructed functional peptide database to determine the functional peptides in the target protein, wherein the functional peptide database is constructed based on a variety of different functional peptide sequences.

6. The method for screening functional peptides according to claim 1, characterized in that: After determining the target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides, the method further includes: Based on identifying the positions of the overlapping colors in the overlapping sequence, the position information of the target peptide segment in the amino acid sequence of the target protein is determined.

7. A functional peptide screening device, characterized in that: include: A functional peptide labeling module, used to determine the functional peptides in the target protein, and label the sequence of the functional peptides in the amino acid sequence of the target protein based on a first color set to obtain a first labeling sequence, wherein the color categories in the first color set correspond one-to-one to the functional categories of the functional peptides; an enzyme-cleaved peptide segment annotation module, for determining the enzyme-cleaved peptide segments in the target protein, and marking the sequences of the enzyme-cleaved peptide segments in the amino acid sequence based on a second color set to obtain a second labeled sequence, wherein the enzyme-cleaved peptide segments are peptide segments obtained by enzymatically hydrolyzing the target protein based on different enzyme-cleavage methods, the color categories in the second color set correspond one-to-one to each enzyme-cleavage method, and the colors in the second color set are different from the colors in the first color set; The screening module is used to overlap the first marker sequence and the second marker sequence to obtain an overlapping sequence, and determine the target peptides with multiple functions in the target protein and the enzyme cleavage method of the target peptides based on the overlapping colors in the overlapping sequence.

8. An electronic device comprising a memory, a processor, and a computer program stored in the memory and running on the processor, characterized in that: When the processor executes the computer program, the screening method for the functional peptide according to any one of claims 1 to 6 is implemented.

9. A non-transitory computer-readable storage medium having a computer program stored thereon, characterized in that: When the computer program is executed by a processor, the method for screening functional peptides according to any one of claims 1 to 6 is implemented.

10. A computer program product, comprising a computer program, characterized in that When the computer program is executed by a processor, the method for screening functional peptides according to any one of claims 1 to 6 is implemented.

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