Process for preparing lignocellulose feed

Abstract

The invention belongs to the technical field of biological organic feed preparation, and particularly relates to a process for preparing lignocellulose feed, which comprises the following steps: S1, preparing cellulose mixed liquid; s2, carrying out alkali treatment on the cellulose mixed solution; s3, preparing a modified lignocellulose solution; s4, carrying out nutrition treatment on the modified lignocellulose solution; and S5, mixing the nutritional modified lignocellulose with a basal feed, and adding 2-4 parts of a bean bag leavening agent for fermentation to finally obtain the lignocellulose feed. The lignocellulose is modified by the decomposition modification liquid, so that the lignocellulose can be decomposed into polysaccharide and organic feed, and the problems of feed shortage and waste of waste wood residue resources in animal husbandry production can be solved. The operation process is simple and convenient, the experimental conditions are simple, and the obtained lignocellulose feed is high in safety and nutritional value.

Description

Technical Field

[0001] The invention belongs to the technical field of biological organic feed preparation, and specifically relates to a process for preparing lignocellulose feed. Background Art

[0002] Wood is a traditional building material and is widely used in modern buildings. Therefore, a lot of waste wood residue is often generated in decoration waste. In addition, industrial production also produces a lot of wood residue waste such as scraps. Every year, a lot of waste wood residue is thrown away because it has no use value. The random disposal of waste wood residue will cause serious environmental problems. In addition, waste wood residue can be recycled, and random disposal will also cause a waste of resources. Therefore, it is urgent to explore the utilization value of wood residue, reuse waste wood residue, and solve the problem of waste wood residue accumulation and disposal.

[0003] At present, the proportion of feed grain in my country's total grain output has increased from 20% in the 1950s to 40%. It is predicted that by 2030, the demand for feed grain in my country will account for 56.5% of the total grain demand. Waste wood residue contains a high amount of cellulose. If the waste wood residue cellulose can be effectively degraded into glucose and easily digestible low-molecular carbohydrates, and then converted into organic feed, it will help solve the problems of feed shortage and waste of waste wood residue resources in animal husbandry production. In the prior art, the methods for processing waste wood residue into feed mainly include physical method, chemical method and microbial method, which have the following defects respectively: the physical method has high requirements for equipment and energy consumption; the treatment of chemical reagents in the chemical method will affect the palatability of wood residue, easily cause environmental pollution, and also pose a potential threat to animal health; the biological treatment method is a mild and environmentally friendly wood residue treatment method, but the fermentation time of the prior art is long and requires a fermentation site. Due to the high water content of the product and the strict requirements for anaerobic conditions, it is prone to secondary fermentation and mold contamination during storage, and the product quality is uncontrollable. It is more suitable for large-scale farms to produce it by themselves, and commercial production cannot be achieved; in the prior art, the action mechanism of the composite treatment method is relatively complicated and difficult to put into actual production.

[0004] Therefore, it is an urgent problem to propose a process for preparing lignocellulose feed to solve the above-mentioned defects. Summary of the invention

[0005] In order to overcome the deficiencies of the prior art, the present invention aims to provide a process for preparing lignocellulose feed.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A process for preparing lignocellulose feed comprises the following steps:

[0008] S1. Preparation of cellulose mixture: According to the mass fraction, 70-90 parts of wood cellulose powder are placed in a reaction vessel, 180-220 parts of deionized water are added, and the mixture is heated at 90-100 ° C and cooked for 20-25 min to obtain a cellulose mixture;

[0009] S2. Alkali treatment of the cellulose mixture: the cellulose mixture was cooked, 5-8 parts of sodium hydroxide was added while cooking, the cooking temperature was 90-100 ° C, the time was 30-50min, cooked until viscous, the cooking was stopped, and the cellulose mixture after alkali treatment was obtained;

[0010] S3. Preparation of modified lignocellulose solution:

[0011] S301. Adding a decomposition and modification liquid to the cellulose mixture after the alkali treatment and stirring, heating in a water bath at 70-80° C. for 1-3 h to obtain a preliminary modified lignocellulose solution;

[0012] S302. Add 11-15 parts of ethanol solution to the preliminary modified lignocellulose solution, and separate using a separatory funnel to obtain a modified lignocellulose solution;

[0013] S4. Nutrition of modified lignocellulose solution: adding a nutrient to the modified lignocellulose solution and stirring to obtain a nutrient-modified lignocellulose;

[0014] S5. Preparation of lignocellulose feed: The nutritious modified lignocellulose is mixed with a basic feed, and 2-4 portions of soybean bag fermentation agent are added for fermentation to finally obtain lignocellulose feed.

[0015] Preferably, in step S301, the preparation of the decomposition and modification liquid comprises the following steps:

[0016] S311. The brown rot fungus was inoculated onto the culture medium and cultured in a constant temperature incubator at 35-37°C for 10-12h. The brown rot fungus cultured on the slant was selected and inoculated into the sterilized MRS liquid culture medium and cultured in a constant temperature incubator at 35-37°C for 10-12h to obtain a seed solution of brown rot fungus;

[0017] S312. Add 2-5 parts of 35% calcium bromide solution and 1-3 parts of 40% dilute sulfuric acid solution to 15-18 parts of brown rot fungus seed liquid and stir to finally obtain a decomposition and modification liquid.

[0018] Preferably, in step S4, the mass ratio of the nutrient agent to the modified lignocellulose solution is 0.045-0.055:1.

[0019] Preferably, in step S4, the nutrient comprises the following components in parts by mass:

[0020] 1-3 parts of zinc sulfate, 2-3 parts of copper chloride, 2-4 parts of magnesium chloride, 0.5-2 parts of calcium hydrogen phosphate, 4-5 parts of vitamin A, 1-2 parts of vitamin D and 3-5 parts of flaxseed oil.

[0021] Preferably, in step S5, the basic feed is one or more combinations of chicken feed, rice husks, and corn.

[0022] Preferably, in step S5, the mixing ratio of the nutritious modified lignocellulose to the basic feed is 1:3-5.

[0023] Preferably, in step S4, the stirring speed is 350-450 r / min and the time is 30-40 min.

[0024] Preferably, in step S302, the concentration of the ethanol solution is 40%.

[0025] Preferably, in step S301, the stirring speed is 450-550 r / min.

[0026] Preferably, in step S312, the concentrations of the calcium bromide solution and the dilute sulfuric acid solution are 35% and 40%, respectively.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] 1. The present invention can decompose lignocellulose into polysaccharides and organic feed by modifying lignocellulose with decomposition modification liquid, which helps to solve the problems of feed shortage and waste of waste wood residue resources in animal husbandry production.

[0029] 2. The operation process of the present invention is relatively simple, and the experimental conditions are relatively simple, and the obtained lignocellulose feed has high safety and nutritional value. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 A diagram showing the steps of preparing lignocellulose feed according to the present invention;

[0031] Figure 2 The actual demonstration diagram of the preparation process of the lignocellulose feed of the present invention is as follows: Figure A is lignocellulose powder; Figure B is the cellulose mixture after the treatment in step S2; and Figure C is the lignocellulose feed fermented in step S5. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] See also Figure 1-2 , the present invention provides a technical solution:

[0034] Example 1

[0035] A process for preparing lignocellulosic feed:

[0036] S1. Preparation of cellulose mixture: According to the mass fraction, 70g of wood cellulose powder was placed in a reaction vessel, 180ml of deionized water was added, and the mixture was heated at 90°C and cooked for 20min to obtain a cellulose mixture;

[0037] S2. Alkali treatment of the cellulose mixture: The cellulose mixture was cooked, 5 g of sodium hydroxide was added while cooking, the cooking temperature was 90 ° C, the time was 30 min, cooked until viscous, the cooking was stopped, and the cellulose mixture after alkali treatment was obtained;

[0038] S3. Preparation of decomposition modification liquid:

[0039] S301. The brown rot fungus was inoculated onto the culture medium and cultured in a 35°C constant temperature incubator for 10 h. The brown rot fungus cultured on the slant was selected and inoculated into the sterilized MRS liquid culture medium and cultured in a 35°C constant temperature incubator for 10 h to obtain a brown rot fungus seed solution.

[0040] S302. Add 2 g of 35% calcium bromide solution and 1 g of 40% dilute sulfuric acid solution to 15 g of brown rot fungus seed solution and stir to obtain a decomposition-modified solution;

[0041] S4. Preparation of modified lignocellulose solution:

[0042] S401. Adding the decomposition and modification liquid to the cellulose mixture after the alkali treatment and stirring at a speed of 450 r / min, heating in a water bath at 70° C. for 1 h to obtain a preliminary modified lignocellulose solution;

[0043] S402. 11 g of 40% ethanol solution was added to the preliminarily modified lignocellulose solution, and the mixture was separated by a separatory funnel to obtain a modified lignocellulose solution;

[0044] S5. Nutrition of modified lignocellulose solution: 45g of nutrient was added to the modified lignocellulose solution and stirred at a stirring speed of 350r / min for 30min to obtain a nutrient-modified lignocellulose;

[0045] S6. Preparation of lignocellulose feed: The nutritious modified lignocellulose is mixed with a basic feed, and 2 g of a bean bag fermentation agent is added for fermentation to finally obtain a lignocellulose feed.

[0046] The nutrient comprises the following components by weight, and 45 g thereof is used in the above preparation process:

[0047] Components Mass(g) Zinc sulfate 10 Cupric chloride 20 Magnesium chloride 20 Calcium Hydrogen Phosphate 5 Vitamin A 40 Vitamin D 10 Flaxseed Oil 30

[0048] Example 2

[0049] A process for preparing lignocellulosic feed:

[0050] S1. Preparation of cellulose mixture: According to the mass fraction, 90g of wood cellulose powder was placed in a reaction vessel, 220ml of deionized water was added, and the mixture was heated at 100°C and cooked for 25min to obtain a cellulose mixture;

[0051] S2 Alkali treatment of the cellulose mixture: the cellulose mixture is boiled, and 8g of sodium hydroxide is added during the boiling process. The boiling temperature is 100°C for 50 minutes. The mixture is boiled until it becomes viscous, and the boiling is stopped to obtain an alkali-treated cellulose mixture.

[0052] S3. Preparation of decomposition modification liquid:

[0053] S301. The brown rot fungus was inoculated onto the culture medium and cultured in a 37°C constant temperature incubator for 12 h. The brown rot fungus cultured on the slant was selected and inoculated into the sterilized MRS liquid culture medium and cultured in a 37°C constant temperature incubator for 12 h to obtain a brown rot fungus seed solution.

[0054] S302. 5g35% calcium bromide solution and 3g40% dilute sulfuric acid solution were added to 18g of brown rot fungus seed solution and stirred to obtain a decomposed modified solution;

[0055] S4. Preparation of modified lignocellulose solution:

[0056] S401. Adding the decomposition and modification liquid to the cellulose mixture after the alkali treatment and stirring at a speed of 550 r / min, heating in a water bath at 80° C. for 3 h to obtain a preliminary modified lignocellulose solution;

[0057] S402. 15 g of 40% ethanol solution was added to the preliminary modified lignocellulose solution, and separated using a separatory funnel to obtain a modified lignocellulose solution;

[0058] S5. Nutrition of modified lignocellulose solution: 55g of nutrient was added to the modified lignocellulose solution and stirred at a stirring speed of 450r / min for 40min to obtain a nutrient-modified lignocellulose;

[0059] S6 Preparation of lignocellulose feed: The nutritious modified lignocellulose is mixed with the basic feed, and 4 g of bean bag fermentation agent is added for fermentation to finally obtain lignocellulose feed.

[0060] The nutrient comprises the following components by weight, and 55 g of them are used in the above preparation process:

[0061] Components Mass(g) Zinc sulfate 30 Cupric chloride 30 Magnesium chloride 40 Calcium Hydrogen Phosphate 20 Vitamin A 50 Vitamin D 20 Flaxseed Oil 50

[0062] Example 3

[0063] A process for preparing lignocellulosic feed:

[0064] S1. Preparation of cellulose mixture: According to the mass fraction, 80g of wood cellulose powder was placed in a reaction vessel, 200ml of deionized water was added, and the mixture was heated at 94°C and cooked for 22min to obtain a cellulose mixture;

[0065] S2. Alkali treatment of the cellulose mixture: The cellulose mixture was cooked, 6 g of sodium hydroxide was added while cooking, the cooking temperature was 95 ° C, the time was 40 min, cooked until viscous, the cooking was stopped, and the cellulose mixture after alkali treatment was obtained;

[0066] S3. Preparation of decomposition modification liquid:

[0067] S301. The brown rot fungus was inoculated onto the culture medium and cultured in a 36°C constant temperature incubator for 11 h. The brown rot fungus cultured on the slant was selected and inoculated into the sterilized MRS liquid culture medium and cultured in a 36°C constant temperature incubator for 11 h to obtain a brown rot fungus seed solution.

[0068] S302. 3g35% calcium bromide solution and 2g40% dilute sulfuric acid solution were added to 16g of brown rot fungus seed solution and stirred to obtain a decomposition modified solution;

[0069] S4. Preparation of modified lignocellulose solution:

[0070] S401. Add the decomposition and modification liquid to the cellulose mixture after the alkali treatment and stir at a speed of 500 r / min, and heat in a water bath at 75° C. for 2 h to obtain a preliminary modified lignocellulose solution;

[0071] S402. 12 g of 40% ethanol solution was added to the preliminary modified lignocellulose solution, and separated using a separatory funnel to obtain a modified lignocellulose solution;

[0072] S5. Nutrition of modified lignocellulose solution: 50 g of nutrient was added to the modified lignocellulose solution and stirred at a stirring speed of 400 r / min for 35 min to obtain a nutrient-modified lignocellulose;

[0073] S6. Preparation of lignocellulose feed: The nutritious modified lignocellulose is mixed with a basic feed, and 3 g of soybean bag fermentation agent is added for fermentation to finally obtain lignocellulose feed.

[0074] The nutrient comprises the following components by weight, and 50 g of them are used in the above preparation process:

[0075] Components Mass(g) Zinc sulfate 20 Cupric chloride 25 Magnesium chloride 30 Calcium Hydrogen Phosphate 10 Vitamin A 45 Vitamin D 15 Flaxseed Oil 40

[0076] Example 4

[0077] A process for preparing lignocellulosic feed:

[0078] S1. Preparation of cellulose mixed solution: According to the mass fraction, 85g of wood cellulose powder was placed in a reaction vessel, 210ml of deionized water was added, and the mixture was heated at 98°C and cooked for 24min to obtain a cellulose mixed solution;

[0079] S2. Alkali treatment of the cellulose mixture: The cellulose mixture was cooked, 7 g of sodium hydroxide was added while cooking, the cooking temperature was 97 ° C, the time was 45 min, cooked until viscous, the cooking was stopped, and the cellulose mixture after alkali treatment was obtained;

[0080] S3. Preparation of decomposition modification liquid:

[0081] S301. The brown rot fungus was inoculated onto the culture medium and cultured in a 36°C constant temperature incubator for 11.5 h. The brown rot fungus cultured on the slant was selected and inoculated into the sterilized MRS liquid culture medium and cultured in a 36°C constant temperature incubator for 11.5 h to obtain a brown rot fungus seed solution.

[0082] S302. 4g35% calcium bromide solution and 2.5g40% dilute sulfuric acid solution were added to 17g of brown rot fungus seed solution and stirred to obtain a decomposition modified solution;

[0083] S4. Preparation of modified lignocellulose solution:

[0084] S401. Adding the decomposition and modification liquid to the cellulose mixture after the alkali treatment and stirring at a speed of 530 r / min, heating in a water bath at 77° C. for 2.5 h to obtain a preliminary modified lignocellulose solution;

[0085] S402. 14 g of 40% ethanol solution was added to the preliminary modified lignocellulose solution, and separated by a separatory funnel to obtain a modified lignocellulose solution;

[0086] S5. Nutrition of modified lignocellulose solution: 52g of nutrient was added to the modified lignocellulose solution and stirred at a stirring speed of 430r / min for 38min to obtain a nutrient-modified lignocellulose;

[0087] S6. Preparation of lignocellulose feed: The nutritious modified lignocellulose is mixed with a basic feed, and 2.5 g of a bean bag fermentation agent is added for fermentation to finally obtain a lignocellulose feed.

[0088] The nutrient comprises the following components by weight, and 52 g is taken therefrom for the above preparation process:

[0089]

[0090]

[0091] Comparative Example 1

[0092] A process for preparing lignocellulosic feed:

[0093] S1. Preparation of cellulose mixture: According to the mass fraction, 70g of wood cellulose powder was placed in a reaction vessel, 180ml of deionized water was added, and the mixture was heated at 90°C and cooked for 20min to obtain a cellulose mixture;

[0094] S2. Alkali treatment of the cellulose mixture: The cellulose mixture was cooked, 5 g of sodium hydroxide was added while cooking, the cooking temperature was 90 ° C, the time was 30 min, cooked until viscous, the cooking was stopped, and the cellulose mixture after alkali treatment was obtained;

[0095] S3. Preparation of decomposition modification liquid:

[0096] S301. The brown rot fungus was inoculated onto the culture medium and cultured in a 35°C constant temperature incubator for 10 h. The brown rot fungus cultured on the slant was selected and inoculated into the sterilized MRS liquid culture medium and cultured in a 35°C constant temperature incubator for 10 h to obtain a brown rot fungus seed solution.

[0097] S302. 3 g of deionized water was added to 15 g of brown rot fungus seed solution and stirred to obtain a decomposed modified solution;

[0098] Preparation of S4 modified lignocellulose solution:

[0099] S401. Adding the decomposition and modification liquid to the cellulose mixture after the alkali treatment and stirring at a speed of 450 r / min, heating in a water bath at 70° C. for 1 h to obtain a preliminary modified lignocellulose solution;

[0100] S402. 11 g of 40% ethanol solution was added to the preliminarily modified lignocellulose solution, and the mixture was separated by a separatory funnel to obtain a modified lignocellulose solution;

[0101] S5. Nutrition of modified lignocellulose solution: 45g of nutrient was added to the modified lignocellulose solution and stirred at a stirring speed of 350r / min for 30min to obtain a nutrient-modified lignocellulose;

[0102] S6. Preparation of lignocellulose feed: The nutritious modified lignocellulose is mixed with a basic feed, and 2 g of a bean bag fermentation agent is added for fermentation to finally obtain a lignocellulose feed.

[0103] The nutrient comprises the following components by weight, and 45 g thereof is used in the above preparation process:

[0104] Components Mass(g) Zinc sulfate 10 Cupric chloride 20 Magnesium chloride 20 Calcium Hydrogen Phosphate 5 Vitamin A 40 Vitamin D 10 Flaxseed Oil 30

[0105] Comparative Example 2

[0106] A process for preparing lignocellulosic feed:

[0107] S1. Preparation of cellulose mixture: According to the mass fraction, 70g of wood cellulose powder was placed in a reaction vessel, 180ml of deionized water was added, and the mixture was heated at 90°C and cooked for 20min to obtain a cellulose mixture;

[0108] S2. Alkali treatment of the cellulose mixture: The cellulose mixture was cooked, 5 g of sodium hydroxide was added while cooking, the cooking temperature was 90 ° C, the time was 30 min, cooked until viscous, the cooking was stopped, and the cellulose mixture after alkali treatment was obtained;

[0109] S3. Preparation of decomposition modified liquid: 2g35% calcium bromide solution and 1g40% dilute sulfuric acid solution were added to 15g of deionized water and stirred to obtain a decomposition modified liquid;

[0110] S4. Preparation of modified lignocellulose solution:

[0111] S401. Adding the decomposition and modification liquid to the cellulose mixture after the alkali treatment and stirring at a speed of 450 r / min, heating in a water bath at 70° C. for 1 h to obtain a preliminary modified lignocellulose solution;

[0112] S402. 11 g of 40% ethanol solution was added to the preliminarily modified lignocellulose solution, and the mixture was separated by a separatory funnel to obtain a modified lignocellulose solution;

[0113] S5. Nutrition of modified lignocellulose solution: 45g of nutrient was added to the modified lignocellulose solution and stirred at a stirring speed of 350r / min for 30min to obtain a nutrient-modified lignocellulose;

[0114] S6. Preparation of lignocellulose feed: The nutritious modified lignocellulose is mixed with a basic feed, and 2 g of a bean bag fermentation agent is added for fermentation to finally obtain a lignocellulose feed.

[0115] The nutrient comprises the following components by weight, and 45 g thereof is used in the above preparation process:

[0116] Components Mass(g) Zinc sulfate 10 Cupric chloride 20 Magnesium chloride 20 Calcium Hydrogen Phosphate 5 Vitamin A 40 Vitamin D 10 Flaxseed Oil 30

[0117] Data Analysis:

[0118] According to the national standard GB / T 5917.1 "Standard for Fermented Biological Feed", the appearance, fermentation effect and decomposition degree of Examples 1-4 and Comparative Examples 1-2 were tested, and the test results are shown in Table 1 below:

[0119] Table 1

[0120]

[0121]

[0122] Feed product feeding test: 500 kg of the lignocellulose feed obtained in Example 1 was prepared and used to feed five selected adult black pigs in good physical condition and without disease. The lignocellulose feed obtained in Example 1 was used to feed the five adult black pigs for the first 30 days of feeding. The weight change data of the five adult black pigs are shown in Table 2 below:

[0123] Table 2

[0124]

[0125] The experimental data of the appearance and fermentation effect of the above-mentioned Examples 1-4 and Comparative Examples 1-2 show that the present invention successfully decomposes and ferments lignocellulose, thereby providing a new feed based on lignocellulose. Compared with Example 1, Comparative Example 1 omits the addition of calcium bromide solution and dilute sulfuric acid solution in the decomposition and modification liquid, and Comparative Example 2 omits the addition of brown rot fungus seed liquid compared with Example 1. Through data analysis, it can be seen that the product effect obtained by Comparative Examples 1-2 does not reach the effect of Examples 1-4, so the interaction of each solution in the decomposition and modification liquid can achieve the optimal effect of the experiment, so that the present invention helps to solve the problems of feed shortage and waste of waste wood residue resources in animal husbandry production, and the present invention successfully applies the lignocellulose feed prepared in Example 1 to five adult black pigs, and the weight of the black pigs increases within 3-30 days of feeding, and the health of the body is good, which better illustrates the practicality of the present invention, so that the present invention has a wider application prospect and economic value.

[0126] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A process for preparing lignocellulosic feed, characterized in that: The following steps are involved: S1. Preparation of cellulose mixture: According to the mass fraction, 70-90 parts of wood cellulose powder are placed in a reaction vessel, 180-220 parts of deionized water are added, and the mixture is heated at 90-100 ° C and cooked for 20-25 min to obtain a cellulose mixture; S2. Alkali treatment of the cellulose mixture: the cellulose mixture was cooked, 5-8 parts of sodium hydroxide was added while cooking, the cooking temperature was 90-100 ° C, the time was 30-50min, cooked until viscous, the cooking was stopped, and the cellulose mixture after alkali treatment was obtained; S3. Preparation of modified lignocellulose solution: S301. Adding the decomposition and modification liquid to the cellulose mixture after the alkali treatment and stirring, heating in a water bath at 70-80° C. for 1-3 h to obtain a preliminary modified lignocellulose solution; S302. Add 11-15 parts of ethanol solution to the preliminary modified lignocellulose solution, and separate using a separatory funnel to obtain a modified lignocellulose solution; S4. Nutrition of modified lignocellulose solution: adding a nutrient to the modified lignocellulose solution and stirring to obtain a nutrient-modified lignocellulose; S5. Preparation of lignocellulose feed: The nutritious modified lignocellulose is mixed with a basic feed, and 2-4 portions of bean bag fermentation agent are added for fermentation to finally obtain lignocellulose feed.

2. A process for preparing lignocellulose feed according to claim 1, characterized in that: In step S301, the preparation of the decomposition and modification liquid includes the following steps: S311. The brown rot fungus was inoculated onto the culture medium and cultured in a constant temperature incubator at 35-37°C for 10-12h. The brown rot fungus cultured on the slant was selected and inoculated into the sterilized MRS liquid culture medium and cultured in a constant temperature incubator at 35-37°C for 10-12h to obtain a seed solution of brown rot fungus; S312. Add 2-5 parts of 35% calcium bromide solution and 1-3 parts of 40% dilute sulfuric acid solution to 15-18 parts of brown rot fungus seed liquid and stir to finally obtain a decomposition and modification liquid.

3. A process for preparing lignocellulose feed according to claim 1, characterized in that: In step S4, the mass ratio of the nutrient agent to the modified lignocellulose solution is 0.045-0.055:

1.

4. A process for preparing lignocellulose feed according to claim 1, characterized in that: In step S4, the nutrient comprises the following components in parts by mass: 1-3 parts of zinc sulfate, 2-3 parts of copper chloride, 2-4 parts of magnesium chloride, 0.5-2 parts of calcium hydrogen phosphate, 4-5 parts of vitamin A, 1-2 parts of vitamin D and 3-5 parts of flaxseed oil.

5. A process for preparing lignocellulose feed according to claim 1, characterized in that: In step S5, the basic feed is one or more combinations of chicken feed, rice husks, and corn.

6. A process for preparing lignocellulose feed according to claim 1, characterized in that: In step S5, the mixing ratio of the nutritious modified lignocellulose and the basic feed is 1:3-5.

7. A process for preparing lignocellulose feed according to claim 1, characterized in that: In step S4, the stirring speed is 350-450 r / min and the stirring time is 30-40 min.

8. A process for preparing lignocellulose feed according to claim 1, characterized in that: In step S302, the concentration of the ethanol solution is 40%.

9. A process for preparing lignocellulose feed according to claim 1, characterized in that: In step S301, the stirring speed is 450-550 r / min.

10. A process for preparing lignocellulose feed according to claim 2, characterized in that: In step S312, the concentrations of the calcium bromide solution and the dilute sulfuric acid solution are 35% and 40%, respectively.

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