SNP (Single Nucleotide Polymorphism) molecular marker related to barley salt tolerance character and application

Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the salt-tolerant character of barley, a primer or a probe, a KASP primer of the SNP molecular marker related to the salt-tolerant character of barley, a barley SNP chip, a reagent or a kit, a barley haplotype and application. The SNP molecular marker and the KASP primer provided by the invention have the advantages that the identification efficiency and accuracy of the barley salt-tolerant variety can be improved, the application range is wide, and the SNP molecular marker and the KASP primer are not influenced by the environment.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to SNP molecular markers related to salt tolerance traits of barley and applications thereof. Background Art

[0002] Soil salinization severely restricts crop growth and poses a huge threat to global food production and the ecological environment. There are large areas of saline-alkali land in many regions of my country, and the land is expanding under the background of global climate change, which further exacerbates the contradiction between a large population and limited land in my country. The idea of ​​saline-alkali land management and utilization in my country has gradually changed from "land modification for planting" to "plant modification for land", and further breakthroughs have been sought in the selection and breeding of salt-tolerant grain, oil, and economic crops. Barley (Hordeum vulgare L.) is an annual or perennial herb of the genus Hordeum in the Poaceae family. It is one of the earliest grains planted by humans. It has the characteristics of early maturity, drought tolerance, salt tolerance, low temperature and cold tolerance, and barrenness tolerance. It is a typical model of salt-tolerant crops. At present, the detailed map of barley whole genome sequencing has been completed. Therefore, screening barley salt-tolerant varieties, exploring new barley salt-tolerant genes, developing molecular markers closely related to salt-tolerant traits, and creating new crop germplasms with high salt tolerance will help increase the increase in cultivated land, ensure food security, and produce good economic and ecological benefits.

[0003] The identification methods of barley salt tolerance mainly include direct identification method, which uses salt solutions of different concentrations to treat plants at the bud and seedling stages in the laboratory, and analyzes morphological data to evaluate their salt tolerance. Physiological and biochemical identification method, the content of various substances in plants changes under salt stress, and the salt tolerance of crops is identified by measuring their changes.

[0004] More specifically, the existing direct identification method usually uses salt solutions of different concentrations to treat plants with salt stress in the laboratory. For example, during the bud stage, the germination potential, germination rate, germination index, germination vitality index and relative growth rate of seedlings are investigated; during the seedling stage, growth conditions such as plant height, root length, fresh weight, dry weight, etc. are investigated, and salt tolerance is assessed based on morphological performance. The disadvantages of the direct identification method include: (1) the samples need to be treated with salt stress, and controls need to be set up, resulting in a long test cycle; the phenotypic investigation of the samples is easily affected by the external environment, such as temperature, humidity, and insect pests, which can affect the growth of the plants, resulting in inaccurate salt tolerance phenotype investigation data, affecting the evaluation of salt tolerance of barley germplasm, and the efficiency of this technology is low for a wide variety of barley varieties. (2) Physiological and biochemical identification method: the operation is cumbersome, which is not conducive to the screening of large quantities of barley varieties and is not efficient. (3) Molecular marker identification: there are too few single SNP markers, and the accuracy rate will be reduced when screening large samples for salt-tolerant germplasm.

[0005] The existing physiological and biochemical identification method is to identify the salt tolerance of crops by measuring the changes of some physiological and biochemical factors in plants under stress conditions, such as betaine and proline, soluble sugar and soluble protein, mineral elements, plasma membrane permeability and malondialdehyde, chlorophyll content, superoxide dismutase activity and malondialdehyde content. The disadvantages of the physiological and biochemical identification method include the need to subject samples to salt stress, the need to set up controls, and a long detection cycle; the operation is cumbersome, requires skilled technical experience, is time-consuming and labor-intensive, is not conducive to the screening of large quantities of barley varieties, and is inefficient.

[0006] Existing methods for identifying salt tolerance in barley also include the use of "genetic factor detection method" to detect control genes related to the genetic mechanism of plant salt tolerance. The DH population constructed by Steptoe / Morex and the DH population constructed by Harrington / TR306 were used to locate QTLs for salt tolerance in barley at the germination stage and seedling stage, respectively. The QTLs related to salt tolerance at the bud stage were detected to be located on chromosomes 4, 6, 7 and chromosomes 5, 7, respectively. The DH population obtained with the salt-tolerant variety CM72 and the salt-sensitive variety Gardner as parents was used as material to locate QTLs for some salt-tolerant related traits using SSR markers, etc. The disadvantages of the above methods include that the existing genetic factor detection technology is mostly based on the parental population constructed from specific salt-tolerant germplasm to find a small number of QTL sites and design markers for tracking and selection. Due to the limitations of genetic selection, it can only be used for individual QTL detection of specific salt-tolerant resources, and it is difficult to be used on a large scale for the screening of massive resources in germplasm resource banks.

[0007] Salt tolerance is a quantitative genetic trait regulated by multiple genes. Positioning and cloning salt tolerance genes is the basis for studying the genetic mechanism of salt tolerance. Traditionally, linkage analysis is used to analyze and map these QTLs and provide useful information on genetic loci. Genome-wide association analysis is a new strategy that uses millions of single nucleotide polymorphisms (SNPs) in the genome as molecular genetic markers to conduct control analysis or correlation analysis at the genome-wide level, and discovers genetic variations that affect complex traits through comparison.

[0008] GWAS has been widely used in the association analysis of excellent traits in crops such as rice, corn, and cotton. After several years of research, GWAS has identified many SNPs and key genes related to plant salt tolerance. These site combinations and salt-tolerant genes can be used to guide marker-assisted breeding and crop improvement. With the completion of the sequencing of the barley genome and the development of barley SNP chip data, the research progress of sites and candidate genes related to barley salt tolerance has been promoted. However, the research on screening genes related to barley salt tolerance using genome-wide association analysis (GWAS) has not yet been perfected. Summary of the invention

[0009] In order to overcome the problems existing in the prior art, the present invention provides SNP molecular markers related to salt tolerance traits of barley and applications thereof.

[0010] The technical solution provided by the present invention is as follows:

[0011] A first aspect of the present invention provides a SNP molecular marker associated with salt tolerance traits of barley, comprising at least one selected from the group consisting of the following SNP molecular markers:

[0012]

[0013] In the present invention, "serial number", "number" and similar words are only used for distinction, and cannot be understood as explicitly or implicitly indicating the relative importance or the number of technical features. For example, the terms "first" and "second" are only used for distinction, and cannot be understood as explicitly or implicitly indicating the relative importance or the number of technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features.

[0014] In some preferred embodiments, the present invention comprises at least one selected from the group consisting of the following 26 SNP molecular markers:

[0015]

[0016] The naming of SNP molecular markers is usually based on the rules or habits when the SNP molecular markers were first discovered. Since DNA is a double-stranded structure and follows the principle of base complementary pairing, the present invention also includes polymorphic forms of SNP molecular markers, that is, reverse complementary sequences or nucleotides of the same double-stranded DNA. For example, the polymorphic form of SNP1 in the present invention is A or G.

[0017] The second aspect of the present invention provides a primer or a probe, comprising a primer or a probe for amplifying or detecting all or part of the above-mentioned SNP molecular markers related to the salt tolerance trait of barley.

[0018] In the present invention, "primers or probes" can be broadly understood as other molecular markers used to amplify or detect all or part of the SNP molecular markers related to the salt tolerance trait of barley described above, that is, including but not limited to primers or probes.

[0019] In some preferred embodiments, the method comprises KASP primers or KASP probes for amplifying or detecting all or part of the above-mentioned SNP molecular markers related to the salt tolerance trait of barley.

[0020] In some more preferred embodiments, the primers of the present invention comprise at least one of the following KASP primers for amplifying or detecting SNP molecular markers related to the salt tolerance trait of barley as described in part of claim 2:

[0021]

[0022]

[0023]

[0024] In the present invention, regarding the numbering of KASP primers, F1 may also be represented by "forward primer 1", F2 may also be represented by "forward primer 2", and R may also be represented by "reverse primer".

[0025] The third aspect of the present invention provides a barley SNP chip, comprising the primers or probes described above.

[0026] The fourth aspect of the present invention provides a reagent or kit comprising primers or probes for amplifying or detecting all or part of the above-mentioned SNP molecular markers related to the salt tolerance trait of barley.

[0027] The fifth aspect of the present invention provides a barley haplotype, comprising the first haplotype and / or the second haplotype, wherein:

[0028] The first type of haplotype includes at least one of Chr4-1, Chr6-1, and Chr7-1;

[0029] The second haplotype includes at least one of Chr4-2, Chr6-2, and Chr7-2;

[0030] The haplotype Chr4-1 includes at least two of SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, and SNP8;

[0031] The haplotype Chr4-2 includes at least two of the polymorphic forms of SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, and SNP8;

[0032] The haplotype Chr6-1 includes at least two of SNP9, SNP10, SNP11, SNP12, SNP13, SNP14, SNP15, SNP16, SNP17, and SNP18;

[0033] The haplotype Chr6-2 includes at least two of the polymorphic forms of SNP9, SNP10, SNP11, SNP12, SNP13, SNP14, SNP15, SNP16, SNP17, and SNP18;

[0034] The haplotype Chr7-1 includes at least two of SNP19, SNP20, SNP21, SNP22, SNP23, SNP24, SNP25, and SNP26;

[0035] The haplotype Chr7-2 includes at least two of the polymorphic forms of SNP19, SNP20, SNP21, SNP22, SNP23, SNP24, SNP25, and SNP26.

[0036] In the present invention, the barley germplasm resources in the first type of haplotype under salt stress show a lower sodium ion content relative to the mean, and the barley germplasm resources in the second type of haplotype under salt stress show a higher sodium ion content relative to the mean. The first type of haplotype is more salt-tolerant (i.e., salt-tolerant type), and the second type of haplotype is more sensitive to salt (salt-sensitive or salt-sensitive).

[0037] In some embodiments, the sodium ion content in the first haplotype is less than 50 mg / g, and the sodium ion content in the second haplotype is greater than 50 mg / g.

[0038] In some embodiments, the barley haplotype comprises any of the following haplotypes,

[0039]

[0040] In some preferred embodiments, a salt-tolerant barley haplotype comprises a first type of haplotype, and optionally a second type of haplotype.

[0041] In the present invention, the term "haplotype" refers to a group of associated multiple SNP allele sites / molecular markers located in a certain region on the same chromosome.

[0042] In some embodiments, the barley haplotype comprises any one of the following haplotype combinations,

[0043]

[0044] In some preferred embodiments, the barley haplotype is a salt-tolerant barley haplotype, comprising at least one of Chr4-1 / / Chr6-1 / / Chr7-1, Chr4-1 / / Chr7-1, Chr7-1, Chr4-1, Chr6-1 / / Chr7-1, Chr4-1 / / Chr6-1, and Chr6-1.

[0045] The sixth aspect of the present invention provides the use of the above-mentioned SNP molecular markers related to the salt tolerance trait of barley, the above-mentioned primers or probes, the above-mentioned barley SNP chip or the above-mentioned reagents or kits in barley genotyping, screening, identification or auxiliary identification, breeding or auxiliary breeding.

[0046] In the present invention, breeding or assisted breeding should be understood in a broad sense, such as may include molecular assisted breeding, QTL analysis, map-based cloning, seed purity detection, assisted major gene selection, backcross breeding, whole genome selection breeding, transgenic component identification, species evolution analysis, etc.

[0047] In some preferred embodiments, the invention comprises any of the following applications:

[0048] (1) Identify or assist in identifying the salt tolerance or salt sensitivity of barley;

[0049] (2) preparing products for identifying or assisting in identifying the salt tolerance or salt sensitivity of barley;

[0050] (3) Comparing the salt tolerance or salt sensitivity of the tested barley varieties;

[0051] (4) preparing a product for comparing the salt tolerance or salt sensitivity of the barley to be tested;

[0052] (5) Breeding barley plants, strains, lines or varieties that are relatively salt tolerant or salt sensitive;

[0053] (6) preparing products for breeding barley plants, strains, lines or varieties with relatively strong salt tolerance or salt sensitivity;

[0054] (7) Screening barley plants with relatively weak or relatively strong salt tolerance or salt sensitivity;

[0055] (8) preparing a product for screening barley plants with relatively weak or relatively strong salt tolerance or salt sensitivity;

[0056] (9) Genotyping of barley germplasm resources and / or genetic populations;

[0057] (10) Constructing DNA fingerprints or databases of barley germplasm resources and / or varieties;

[0058] (11) Conduct genetic diversity analysis on barley germplasm resources and / or genetic populations.

[0059] (12) Application in cluster analysis of barley;

[0060] (13) Application in genome-wide association analysis of barley;

[0061] (14) Application in identification of genetic relationships in barley.

[0062] The beneficial effects of the present invention include at least the following:

[0063] 1) The present invention is based on a core barley germplasm population constructed based on the genetic diversity of the germplasm bank with rich germplasm resources, and uses genome-wide association analysis (GWAS) to screen genes related to barley salt tolerance traits, and obtains 26 new SNP molecular markers related to salt tolerance.

[0064] There is no limit on the scope of barley test materials and the number of samples, which makes it widely applicable.

[0065] 2) The SNP molecular markers related to the salt tolerance trait of barley provided by the present invention can simultaneously detect multiple salt tolerance SNP sites, and are used for germplasm resource screening and salt tolerance breeding of barley.

[0066] The SNP molecular markers provided by the present invention can be used to screen barley germplasm resources with different salt tolerance levels, and different SNP molecular markers can be randomly combined.

[0067] The SNP molecular markers related to barley salt tolerance provided by the present invention are verified in natural populations and the results show that the SNP molecular markers can effectively predict the salt tolerance of barley at the seedling stage, and thus can be used for barley salt tolerance trait screening, molecular marker-assisted breeding, etc.

[0068] 3) The KASP (Kompetitive Allele-Specific PCR) primers or probes provided by the present invention can quickly detect salt-tolerant haplotypes and lock salt-tolerant related genes.

[0069] 4) The SNP molecular markers and KASP primers provided by the present invention can improve the efficiency and accuracy of identifying salt-tolerant barley varieties, reduce the time period for salt-tolerant identification, have a wide range of applications, and are not affected by the environment.

[0070] The SNP molecular markers and KASP primers provided by the present invention can be used for molecular marker-assisted selection or design breeding in salt-tolerant barley variety cultivation. BRIEF DESCRIPTION OF THE DRAWINGS

[0071] Figure 1 It shows that the haplotype combination of the present invention has an effect on the Na content of the aerial part of the barley material under salt stress. + The green one is the excellent haplotype 1 with salt tolerance, and the red one is the excellent haplotype 2 with non-salt tolerance, **p<0.01, *p<0.05.

[0072] Figure 2 A genotyping diagram using KASP primers in an example of the present invention is shown. DETAILED DESCRIPTION

[0073] In order to make the purpose, technical scheme and beneficial effects of the present invention clearer, the present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. Examples of the embodiments are shown in the accompanying drawings. It should be understood that the specific embodiments described in the following embodiments of the present invention are only exemplary descriptions of the specific embodiments of the present invention, and are intended to be used to explain the present invention, but do not constitute a limitation of the present invention.

[0074] The endpoints and any values ​​of the scope disclosed in this article are not limited to the precise scope or value, and these scopes or values ​​should be understood to include values ​​close to these scopes or values. For numerical ranges, between the endpoint values ​​of each scope, between the endpoint values ​​of each scope and a separate point value, and between separate point values, one or more new numerical ranges can be combined with each other, and these numerical ranges should be considered as specifically disclosed in this article. In the description of the application, unless otherwise stated, the meaning of similar words such as "multiple / multiple" is two / kinds or more than two / kinds.

[0075] The present invention is further described in detail below in conjunction with the examples. It should be understood that if the experimental methods in the following examples are not specified with specific conditions, they are usually implemented according to conventional conditions or according to the conditions adopted by the partners.

[0076] Example 1

[0077] The method for developing barley salt-tolerance SNP molecular markers in Example 1 of the present invention mainly comprises the following steps:

[0078] (1) Preparation of barley test materials

[0079] The core group of 288 barley germplasm accessions was constructed by utilizing the genetic diversity of the germplasm bank to enrich the germplasm resources, including 129 domestic and 156 foreign barley accessions, as well as 3 accessions of unknown geographical origin, with a wide range of sources.

[0080] (2) Identification of salt tolerance in barley seedlings

[0081] After the materials to be identified were disinfected and germinated, they were grown in plug trays. When the barley seedlings grew to 1 leaf and 1 heart, they were transplanted into a turnover box filled with nutrient solution. When the barley seedlings grew to 3 leaves and 1 heart, the barley materials were treated with 0mM NaCl (control) and 300mM NaCl (salt treatment). After 2 weeks of salt stress treatment, the aerial parts were sterilized at 105℃ for 3h, dried at 80℃ to constant weight, and crushed. 0.1g of the crushed samples were weighed and completely digested in a microwave subtraction system. The Na + Each test was performed with 3 biological replicates and each replicate was tested twice.

[0082] (3) GWAS analysis and SNP molecular marker determination

[0083] Genome-wide association analysis (GWAS) was used to screen genes related to salt tolerance in the barley seedling stage, and 26 new SNP loci related to salt tolerance were obtained (-LOG10(P) ≥ 4.40), which were located on chromosomes 4, 6 and 7. Two haplotypes were generated on Chr4, Chr6 and Chr7, respectively, among which haplotype 1 was an "excellent haplotype" that can improve salt tolerance.

[0084] (4) Salt-tolerant “excellent haplotype” combination

[0085] Haplotype combinations were performed on the two haplotypes generated on Chr4, Chr6 and Chr7, respectively. Chr4-1, Chr6-1 and Chr7-1 respectively represented excellent haplotypes that could improve salt tolerance formed by the significant salt-tolerant SNP sites on the corresponding chromosomes, and Chr4-2, Chr6-2 and Chr7-2 respectively represented another haplotype formed by the SNP sites on the corresponding chromosomes. A total of 8 haplotype combinations were obtained (as shown in Table 1). + The phenotypic data of the content were analyzed by haplotype combination, and the results were as follows Figure 1 shown.

[0086] Table 1 Combination of haplotypes based on barley haplotype information

[0087]

[0088] (5) Effect of excellent haplotype combinations on salt tolerance

[0089] The aggregation of three superior haplotypes (HC1) significantly improved the salt tolerance of barley compared with the aggregation of three non-superior haplotypes (HC8). The Na content of the aerial parts of the barley material containing HC1 after salt treatment was significantly increased. + Content (such as Figure 1 Barley materials containing one or two salt-tolerant “excellent haplotypes” (HC2-HC7) can significantly reduce Na compared with HC8. + Secondly, HC2, HC3, and HC4 (containing the non-salt-tolerant "excellent haplotype" of Chr6-2) have lower Na content than HC5, HC6, and HC7 (containing the salt-tolerant "excellent haplotype" of Chr6-1). + The results showed that the salt-tolerant "excellent haplotypes" of Chr4-1 and Chr7-1 made up for the weakness of the Chr6-2 haplotype in salt tolerance, and had a stronger ability to improve salt tolerance than the salt-tolerant "excellent haplotype" of Chr6-1.

[0090] (6) Development and detection of molecular markers for barley salt-tolerant haplotype KASP

[0091] The method for identifying the salt tolerance and different levels of salt tolerance of the barley to be tested in the seedlings for the 26 SNP molecular markers in the above haplotypes and their combinations (as shown in Table 2) is to detect the SNP sites and the "excellent haplotype" combination in the barley to be tested. Then, the barley reference genome (Barleycv.Morex IBSC 2V12016) was compared, and 120bp upstream and downstream of the above SNP markers were intercepted, a total of 241bp of sequence, and KASP primers were designed and developed using the online platform SNPWay website (http: / / www.snpway.com / ), of which 23 SNP sites were successfully converted into KASP primers (Table 3). Using the above primers, the variation of the bases at the barley salt tolerance haplotype combination was detected ( Figure 1 ), indicating that the developed KASP primers can clearly distinguish the validation materials.

[0092] Table 2 Information on 26 SNP molecular markers in haplotypes and their combinations

[0093]

[0094]

[0095] Genotyping operation steps: Extract genomic DNA of the sample to be tested according to the operation steps of Shanghai Pudi Plant Genome Extraction Kit. The total KASP reaction system is 10μL, including 2×KASPMaster Mix 5μL, KASPAssayMix (primer mixed working solution) 0.125μL, and 4.875μL of template DNA with a concentration of 20ngμL-1. The KASP reaction program is as follows: step 1: 95℃, 10min; step 2: 95℃, 20s, 61-55℃, 1min, each cycle decreases 0.6℃, for a total of 10 cycles; step 3: 95℃, 20s, 55℃, 1min, for a total of 35 cycles. The PCR product was scanned and analyzed by KASP fluorescence analyzer (LGC model FLUOstar Omega fluorescent plate reader).

[0096] Table 3 KASP molecular marker sequence information is as follows

[0097]

[0098]

[0099]

[0100] (7) Methods for identifying salt tolerance in barley seedlings based on SNP molecular markers, including

[0101] Based on the results in (5), it was found that the combinations of different salt-tolerant haplotypes have different abilities to improve the salt tolerance of barley. The order from high to low in improving the salt tolerance of barley is Chr4-1 / / Chr6-1 / / Chr7-1, Chr4-1 / / Chr7-1, Chr7-1, Chr4-1, Chr6-1 / / Chr7-1, Chr4-1 / / Chr6-1, Chr6-1.

[0102] Using 16 barley germplasm resources as materials, the Na + Content determination and salt-tolerant haplotype combination analysis. As shown in Table 2, it includes 9 salt-tolerant materials and 7 salt-sensitive materials (as shown in Table 4).

[0103] Table 416 barley germplasm materials with different salt tolerance

[0104]

[0105]

[0106] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention and do not constitute a limitation on the content of the present invention. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including the combination of various technical features in any other suitable manner, and these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

Claims

1. A SNP molecular marker related to salt tolerance of barley, characterized in that: Contains at least one selected from the group consisting of the following SNP molecular markers, 。 2. The SNP molecular marker associated with salt tolerance of barley according to claim 1, characterized in that: Contains at least one selected from the group consisting of the following 26 SNP molecular markers, 。 3. A primer or probe, characterized in that The method comprises primers or probes for amplifying or detecting SNP molecular markers related to the salt tolerance trait of barley according to all or part of claim 1 or 2.

4. The primer according to claim 3, characterized in that The method comprises at least one of the following KASP primers for amplifying or detecting the SNP molecular markers related to the salt tolerance trait of barley as described in part of claim 2, 。 5. A barley SNP chip, characterized in that: The invention comprises the primers or probes related to the salt tolerance trait of barley according to any one of claims 3 to 5.

6. A reagent or a kit, characterized in that: The method comprises primers or probes for amplifying or detecting SNP molecular markers related to the salt tolerance trait of barley according to all or part of claim 1 or 2.

7. A barley haplotype, characterized in that: Contains the first type of haplotype and / or the second type of haplotype, wherein, The first type of haplotype includes at least one of Chr4-1, Chr6-1, and Chr7-1; The second haplotype includes at least one of Chr4-2, Chr6-2, and Chr7-2; The haplotype Chr4-1 includes at least two of SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, and SNP8; The haplotype Chr4-2 includes at least two of the polymorphic forms of SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, and SNP8; The haplotype Chr6-1 includes at least two of SNP9, SNP10, SNP11, SNP12, SNP13, SNP14, SNP15, SNP16, SNP17, and SNP18; The haplotype Chr6-2 includes at least two of the polymorphic forms of SNP9, SNP10, SNP11, SNP12, SNP13, SNP14, SNP15, SNP16, SNP17, and SNP18; The haplotype Chr7-1 includes at least two of SNP19, SNP20, SNP21, SNP22, SNP23, SNP24, SNP25, and SNP26; The haplotype Chr7-2 includes at least two of the polymorphic forms of SNP19, SNP20, SNP21, SNP22, SNP23, SNP24, SNP25, and SNP26.

8. The barley haplotype according to claim 7, characterized in that Contains any of the following haplotypes, 。 9. The barley haplotype according to claim 7, characterized in that: Contains any of the following haplotype combinations, 。 10. Use of the SNP molecular marker associated with the salt tolerance trait of barley according to any one of claims 1-2, the primer or probe associated with the salt tolerance trait of barley according to any one of claims 3-4, the barley SNP chip according to claim 5, or the reagent or kit according to claim 6 in barley genotyping, screening, identification or assisted identification, breeding or assisted breeding.

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