Polypeptide composition and application thereof in facial mask

Abstract

The invention discloses a polypeptide composition. The polypeptide composition is prepared from the following components: palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8. The invention further discloses application of the polypeptide composition in preparation of cosmetics or skin care products. In addition, the invention further discloses mask liquid containing the polypeptide composition and a preparation method of the mask liquid. The polypeptide composition is jointly applied to the mask, has a synergistic effect in the aspects of moisturizing, wrinkle removal and the like, and is very suitable for daily skin care.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products, and in particular relates to a polypeptide composition and application thereof in a facial mask. Background Art

[0002] The first step to solve all skin problems is moisturizing. The water content of the stratum corneum plays an important biological regulatory role on the skin and even the entire body. Normally, the water content of the epidermis is about 10%, which varies due to the body's internal factors (gender, age, genetics, nutrition and metabolism, etc.) and living environment (humidity). Properly adjusting the water content of the stratum corneum is beneficial to delay skin aging and prevent and treat certain skin diseases. If moisturizing can be sustained for a long time and can provide a soothing and non-irritating environment for damaged and sensitive skin, reduce skin inflammation, and enhance repair ability, it can take a two-pronged approach to ensure the perfect rejuvenation of the skin. In the field of cosmetics, facial masks seem to be one of the best choices for moisturizing the skin.

[0003] Facial masks are a category of skin care products. They use the short time they cover the face to temporarily isolate the outside air and pollution, increase skin temperature and dilate pores, promote sweat gland secretion and metabolism, and increase skin oxygen content. The moisture in the mask penetrates into the skin's stratum corneum, making it soft and increasing elasticity; the adhesive ingredients or powdered adsorbents in the mask can adhere to dirt, metabolic waste and excess oil on the skin surface and in the pores, and completely remove them. The efficacy of the mask has a lot to do with the active ingredients added to it.

[0004] Peptides are compounds formed by amino acids linked together by peptide bonds. They are also intermediate products of protein hydrolysis and widely participate in human life activities. They are substances with clear efficacy, moderate activity, clear structure, and safety and reliability. Due to their advantages of high safety and strong biological activity, more and more peptides have been widely used in the development of cosmetics and skin care products.

[0005] At present, among the masks containing polypeptide active ingredients, collagen mask products are more common, but since collagen is a macromolecular protein, it is not easy to penetrate into the skin to exert its efficacy. For example, Chinese patent CN111067816A discloses a moisturizing and repairing mask containing active polypeptides and a preparation method thereof, which prepares a mask with the effects of tightening and locking moisture, moisturizing, scavenging free radicals, and maintaining skin whitening by adding a sterile composite polypeptide rich in type III collagen sequence to the mask. However, the molecular weight of the active polypeptide is relatively large, which is not conducive to the active polypeptide penetrating into the skin to exert its efficacy.

[0006] In addition, some existing technologies apply polypeptide compositions to facial masks, but the effects are general or the preparation steps are cumbersome. For example, Chinese patent CN105147534A discloses a polypeptide composition for skin repair, which includes one, two or three of a polypeptide that promotes the production of skin collagen, a polypeptide that promotes the production of skin laminin, a polypeptide that promotes skin elastin, and a polypeptide that compensates for the lack of proteoglycan, so as to activate the regeneration and repair ability of the skin, thereby repairing the skin caused by various factors. Damage, aging, acne scars and other problems, the polypeptide composition can be used to prepare repair cosmetics and medical dressings for treating body surface trauma and reducing scars. However, the polypeptide composition in this patent is mainly used for facial repair, etc., and may not be effective for daily skin care.

[0007] Therefore, developing a facial mask comprising a polypeptide composition and using it in daily skin care has a good market prospect. Summary of the invention

[0008] In order to overcome the various problems existing in existing facial masks, the inventors explored different methods and extensively screened the polypeptides used in different cosmetics. In the research, they unexpectedly found that the use of a specific polypeptide composition in a facial mask has a synergistic effect in terms of moisturizing, etc., and produced unexpected technical effects. Based on this discovery, the present invention provides the following technical solutions.

[0009] In a first aspect, the present invention provides a polypeptide composition comprising the following ingredients: palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8.

[0010] In one or more embodiments, the mass ratio of palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8 in the polypeptide composition is 10:1:1:1:1:2-20:1:1:1:1:3.

[0011] In a second aspect, the present invention provides the use of the polypeptide composition in the preparation of cosmetics or skin care products, wherein the cosmetics or skin care products are essences, creams, facial cleansers, facial milks, facial masks, eye creams or essence waters.

[0012] In one or more embodiments, the amount of the polypeptide composition added to the cosmetics or skin care products is 0.001%-1% by mass.

[0013] In a third aspect, the present invention provides a facial mask liquid, which includes the polypeptide composition of the present invention, a phase A mixture and a phase B mixture, wherein the phase A mixture and the phase B mixture are acceptable excipients and solvents in skin care products.

[0014] In one or more embodiments, the phase A mixture in the mask liquid is butylene glycol, propylene glycol, glyceryl polyether-26, PEG / PPG-17 / 6 copolymer, betaine, erythritol, hydroxyethylpiperazine ethane sulfonic acid, p-hydroxyacetophenone, carbomer, hydrolyzed sclerotium gum, sodium hyaluronate and water; the phase B mixture in the mask is taurine, hydroxyethylpiperazine ethane sulfonic acid, azelaic acid MEA, hydrolyzed collagen zinc, potassium methoxysalicylate, ellagic acid, grape (VITIS VINIFERA) fruit cell extract, butylene glycol, white water lily (NYMPHAEA ALBA) flower extract, beautiful AHNFELTIOPSISCONCINNA extract, white fruit purple grass (LITHOSPERMUM OFFICINALE) root extract, p-hydroxyacetophenone, 1,2-hexanediol, Saururus CHINENSIS flower extract, Portulaca oleracea (PORTULACA OLERACEA extract, glycerin, CHRYSANTHELLUM INDICUM extract, PELARGONIUMGRAVEOLENS flower / leaf / stem extract, MENTHA ARVENSIS leaf extract, ALOEBARBADENSIS extract, SOPHORA FLAVESCENS root extract, hydroxyethyl urea, beta-glucan, ethylhexylglycerin, inositol, polysorbate 20, panthenol, phenoxyethanol, PEG-12 dimethicone crosspolymer, propylene glycol, triethanolamine and water.

[0015] In one or more embodiments, the contents of the ingredients in phase A are, by percentage of the total weight of the facial mask liquid, 3%-5% butylene glycol, 2%-4% propylene glycol, 1%-3% glycerol polyether-26, 1%-3% PEG / PPG-17 / 6 copolymer, 1%-3% betaine, 0.8%-1.2% erythritol, 0.3%-0.8% hydroxyethylpiperazineethane sulfonic acid, 0.1%-0.5% p-hydroxyacetophenone, 0.05%-0.2% carbomer, 0.05%-0.15% hydrolyzed sclerotium gum, 0.01%-0.05% sodium hyaluronate and 74%-75% water.

[0016] In one or more embodiments, the content of each component in the phase B is as follows, based on the total weight of the facial mask liquid: taurine 0.3%-0.7%, hydroxyethylpiperazine ethane sulfonic acid 0.3%-0.6%, azelaic acid MEA 0.2%-0.5%, hydrolyzed collagen zinc 0.2%-0.5%, potassium methoxysalicylate 0.01%-0.1%, ellagic acid 0.0001%-0.1%, grape (VITIS VINIFERA) fruit cell extract 0.00005%-0.1%, butylene glycol 0.3%-0.5%, white water lily (NYMPHAEAALBA) flower extract 0.03%-0.1%, beautiful Ion algae (AHNFELTIOPSIS CONCINNA) extract 0.03%-0.1%, Lithospermum officinale (LITHOSPERMUM OFFICINALE root extract 0.01%-0.1%, parahydroxyacetophenone 0.03%-0.1%, 1,2-hexanediol 0.5%-1%, Saururus CHINENSIS flower extract 0.0003%-0.1%, Portulaca oleracea extract 0.1%-0.3%, glycerin 0.5%-0.8%, Chrysanthellum INDICUM extract 0.05%-0.2%, Pelargonium graveolens flower / leaf / stem extract 0.05%-0.1%, Mentha arnica leaf extract 0.01%-0.05%, Aloe barbadensis extract 0.02%-0.05%, Sophora flavescens extract FLAVESCENS) root extract 0.01%-0.1%, hydroxyethyl urea 0.5%-1.2%, β-glucan 0.01%-0.05%, ethylhexylglycerin 0.001%-0.1%, inositol 0.5%-1.5%, polysorbate-200.01%-0.05%, panthenol 0.3%-1%, phenoxyethanol 0.002%-0.005%, PEG-12 polydimethylsiloxane crosspolymer 0.1%-0.3%, propylene glycol 0.03%-0.06%, triethanolamine 0.03%-0.2%, water 3-4%.

[0017] In a fourth aspect, the present invention provides a method for preparing the facial mask liquid, the method comprising the following steps:

[0018] (1) Add phase A to an emulsifying pot, turn on the stirrer, stir at a speed of 30-35 rpm, and heat to 85-90°C; turn on the homogenizer, homogenize for 3-5 minutes, at a speed of 2000-3000 rpm, and keep warm for 30 minutes.

[0019] (2) Turn on the cooling water, cool down to 40-45°C, add the phase B raw material and the polypeptide composition, stir evenly, and take samples for plate alignment;

[0020] (3) After the plate is qualified, the material is discharged and left to stand, and after passing the inspection, it is filled and packaged, and the finished product is inspected and put into storage.

[0021] In a fifth aspect, the present invention provides a facial mask, comprising the facial mask liquid as described in any one of items and a carrier, wherein the facial mask liquid is attached to the carrier.

[0022] The present invention uses the polypeptide composition in a facial mask, which produces a synergistic effect in terms of moisturizing, wrinkle removal, etc., is very suitable for daily skin care, and has a very good application prospect. In addition, the facial mask production process of the present invention is simple and suitable for mass industrial production. DETAILED DESCRIPTION

[0023] In order to overcome the various problems existing in existing facial masks, the inventors explored different methods and conducted extensive screening of polypeptides used in different cosmetics. During the research, they unexpectedly found that the use of specific polypeptide compositions in facial masks has a synergistic effect in moisturizing, removing wrinkles, etc., producing unexpected technical effects.

[0024] The polypeptide composition of the present invention comprises the following components: palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8.

[0025] Palmitoyl tripeptide-8 is a signal peptide that can promote the synthesis of collagen (type I, II and IV) and the production of extracellular matrix proteins by increasing matrix cell activity, making the skin look younger and more elastic. Adding it to cosmetics can help the skin replenish collagen, improve facial elasticity, and significantly reduce facial wrinkles. At the same time, it also has the characteristics of enhancing cell activity, increasing skin water content, enhancing skin luster, and improving skin color. It increases the water content of the skin and locks in moisture, making the skin feel soft and smooth, and brightens the skin from the inside out, making it no longer dull.

[0026] Palmitoyl tripeptide-1 is a small three amino acid signal peptide with an amino acid sequence of glycine-histidine-lysine (GHK). GHK is attached to palmitic acid (a fatty acid) to increase its skin permeability and oil solubility. Applying palmitoyl tripeptide-1 directly to the skin can stimulate cells to produce more and newer collagen. It takes at least 1 month or more to start seeing visible changes in new collagen. Palmitoyl tripeptide-1 has the effect of anti-wrinkle and anti-aging, and improves skin quality. It acts on the dermis, can promote the synthesis of extracellular matrix such as collagen and glycosaminoglycans, strengthen the dermis, make the skin thicker and firmer, wrinkles are relieved, and the ability to resist ultraviolet radiation is stronger.

[0027] Acetyl Heptapeptide-4, also known as SNAP-8, is a cosmetic ingredient known for its many benefits for hair and skin. This peptide is derived from gamma-aminobutyric acid (GABA), a neurotransmitter found naturally in our bodies. Acetyl Heptapeptide-4 is primarily used in hair care products to stimulate hair growth, strengthen hair follicles, and reduce hair loss. It works by boosting blood circulation in the scalp, allowing essential nutrients to reach the hair follicles and promote healthy hair growth. In addition, it helps inhibit the effects of 5-alpha reductase, an enzyme that causes male pattern baldness and thinning hair. For the skin, Acetyl Heptapeptide-4 is widely used in anti-aging products. It works by relaxing facial muscles and reducing the appearance of fine lines and wrinkles. By blocking the receptors responsible for muscle contraction, it helps prevent the formation of new wrinkles and reduces the appearance of existing wrinkles. In addition, this ingredient stimulates collagen production, which improves the structure of the skin, making it appear firmer and more youthful. Acetyl Heptapeptide-4 is found in a variety of cosmetic products, such as hair conditioners, anti-aging lotions and face creams.

[0028] Oligopeptide-1 is a synthetic polypeptide composed of glycine, histidine and lysine, which has many benefits for our skin: it has a good repair and care effect on damaged skin, sensitive skin and traumatized skin; it reduces the average age of skin tissue; it promotes the synthesis of collagen and collagenase, secretes collagen, hyaluronic acid and glycoprotein, moisturizes the skin, enhances skin elasticity, reduces skin wrinkles and prevents skin aging; it reduces skin pigmentation, so as to achieve the purpose of whitening and removing spots; it repairs damaged cells, reduces the damage of direct ultraviolet rays to the skin, reduces the growth of dark spots on the skin after sun exposure, eliminates genetic mutation factors of damaged cells, and prevents photoaging.

[0029] Heptapeptide-6 is an active peptide that can reduce fat, reduce weight, effectively reduce various wrinkles, and improve skin firmness, smoothness, and elasticity. Heptapeptide-6 is a cosmetic raw material used for weight loss and cellulite removal. It is suitable for various anti-wrinkle and anti-aging care products. It is added to the final stage of cosmetic production and mixed with weight loss products. It is highly safe and has no side effects.

[0030] Acetyl hexapeptide-8, also known as akirelin, is an active polypeptide composed of six amino acids. Acetyl hexapeptide-8 is a neurotransmitter inhibitory peptide and is used in cosmetics. Acetyl hexapeptide-8 competes for SNAP-25 sites in the melt-vesicle complex, thereby affecting the formation of the complex. When the melt-vesicle complex is slightly unstable, the vesicle cannot effectively release neurotransmitters, which leads to weakened muscle contraction and prevents the formation of wrinkles. Therefore, it is a high-quality wrinkle-removing cosmetic raw material with high anti-wrinkle activity and small side effects. Akiralin can locally block the nerve transmission of muscle contraction messages, affect the conduction of skin vesicle nerves, relax facial muscles, and smooth dynamic lines, static lines and fine lines; effectively reorganize collagen elasticity, increase the activity of elastin, relax facial lines, smooth wrinkles and improve relaxation. It can be used in cosmetics as an anti-wrinkle ingredient with excellent results and has been used in various high-end cosmetics series.

[0031] The inventors of the present invention have found in cosmetic research that the combination of palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8 applied to facial masks has a synergistic effect and can significantly enhance the moisturizing effect of the facial mask.

[0032] It should be understood that based on the above disclosed polypeptide composition, those skilled in the art can adjust the usage amount of each polypeptide therein according to needs to adapt to the stability of cosmetics or skin care products, improve the efficacy of facial masks, etc.

[0033] In particular, in one or more embodiments, the mass ratio of palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8 in the polypeptide composition can be 10:1:1:1:1:2-20:1:1:1:1:3.

[0034] It should be understood that the above-mentioned polypeptide composition has a synergistic moisturizing effect, so the composition can be used in the preparation of cosmetics or skin care products, and the cosmetics or skin care products are essences, creams, facial cleansers, facial milk, facial masks, eye creams or essence water.

[0035] It should be understood that based on the above disclosure of the polypeptide composition, those skilled in the art can adjust the dosage of the polypeptide composition as required to adapt to the stability of cosmetics or skin care products, improve the efficacy of facial masks, etc.

[0036] In particular, in one or more embodiments, the amount of the polypeptide composition added to the cosmetic or skin care product is 0.001%-1% by mass.

[0037] In particular, the polypeptide composition of the present invention is suitable for preparing a facial mask liquid, which comprises the polypeptide composition of the present invention, a phase A mixture and a phase B mixture, wherein the phase A mixture and the phase B mixture are acceptable excipients and solvents in skin care products.

[0038] In one or more embodiments, the phase A mixture in the mask liquid is butylene glycol, propylene glycol, glyceryl polyether-26, PEG / PPG-17 / 6 copolymer, betaine, erythritol, hydroxyethylpiperazine ethane sulfonic acid, p-hydroxyacetophenone, carbomer, hydrolyzed sclerotium gum, sodium hyaluronate and water; the phase B mixture in the mask is taurine, hydroxyethylpiperazine ethane sulfonic acid, azelaic acid MEA, hydrolyzed collagen zinc, potassium methoxysalicylate, ellagic acid, grape (VITIS VINIFERA) fruit cell extract, butylene glycol, white water lily (NYMPHAEAALBA) flower extract, beautiful AHNFELTIOPSISCONCINNA extract, white fruit purple grass (LITHOSPERMUM OFFICINALE) root extract, p-hydroxyacetophenone, 1,2-hexanediol, Saururus CHINENSIS flower extract, Portulaca oleracea (PORTULACA OLERACEA extract, glycerin, CHRYSANTHELLUM INDICUM extract, PELARGONIUMGRAVEOLENS flower / leaf / stem extract, MENTHAARVENSIS leaf extract, ALOEBARBADENSIS extract, SOPHORA FLAVESCENS root extract, hydroxyethyl urea, beta-glucan, ethylhexylglycerin, inositol, polysorbate 20, panthenol, phenoxyethanol, PEG-12 dimethicone crosspolymer, propylene glycol, triethanolamine and water.

[0039] In one or more embodiments, the contents of the ingredients in phase A are, by percentage of the total weight of the facial mask liquid, 3%-5% butylene glycol, 2%-4% propylene glycol, 1%-3% glycerol polyether-26, 1%-3% PEG / PPG-17 / 6 copolymer, 1%-3% betaine, 0.8%-1.2% erythritol, 0.3%-0.8% hydroxyethylpiperazineethane sulfonic acid, 0.1%-0.5% p-hydroxyacetophenone, 0.05%-0.2% carbomer, 0.05%-0.15% hydrolyzed sclerotium gum, 0.01%-0.05% sodium hyaluronate and 74%-75% water.

[0040] In one or more embodiments, the content of each component in the phase B is as follows, based on the total weight of the facial mask liquid: taurine 0.3%-0.7%, hydroxyethylpiperazine ethane sulfonic acid 0.3%-0.6%, azelaic acid MEA 0.2%-0.5%, hydrolyzed collagen zinc 0.2%-0.5%, potassium methoxysalicylate 0.01%-0.1%, ellagic acid 0.0001%-0.1%, grape (VITIS VINIFERA) fruit cell extract 0.00005%-0.1%, butylene glycol 0.3%-0.5%, white water lily (NYMPHAEAALBA) flower extract 0.03%-0.1%, beautiful Ion algae (AHNFELTIOPSIS CONCINNA) extract 0.03%-0.1%, Lithospermum officinale (LITHOSPERMUM OFFICINALE root extract 0.01%-0.1%, parahydroxyacetophenone 0.03%-0.1%, 1,2-hexanediol 0.5%-1%, Saururus CHINENSIS flower extract 0.0003%-0.1%, Portulaca oleracea extract 0.1%-0.3%, glycerin 0.5%-0.8%, Chrysanthellum INDICUM extract 0.05%-0.2%, Pelargonium graveolens flower / leaf / stem extract 0.05%-0.1%, Mentha arnica leaf extract 0.01%-0.05%, Aloe barbadensis extract 0.02%-0.05%, Sophora flavescens extract FLAVESCENS) root extract 0.01%-0.1%, hydroxyethyl urea 0.5%-1.2%, β-glucan 0.01%-0.05%, ethylhexylglycerin 0.001%-0.1%, inositol 0.5%-1.5%, polysorbate-200.01%-0.05%, panthenol 0.3%-1%, phenoxyethanol 0.002%-0.005%, PEG-12 polydimethylsiloxane crosspolymer 0.1%-0.3%, propylene glycol 0.03%-0.06%, triethanolamine 0.03%-0.2%, water 3-4%.

[0041] In addition, the present invention also provides a method for preparing the facial mask liquid, the method comprising the following steps:

[0042] (1) Add phase A to an emulsifying pot, turn on the stirrer, stir at a speed of 30-35 rpm, and heat to 85-90°C; turn on the homogenizer, homogenize for 3-5 minutes, at a speed of 2000-3000 rpm, and keep warm for 30 minutes.

[0043] (2) Turn on the cooling water, cool down to 40-45°C, add the phase B raw material and the polypeptide composition, stir evenly, and take samples for plate alignment;

[0044] (3) After the plate is qualified, the material is discharged and left to stand, after passing the inspection, it is filled and packaged, and the finished product is inspected and put into storage.

[0045] It should be understood that on the basis of the above disclosed mask liquid ingredients or preparation methods thereof, those skilled in the art can prepare a mask according to the prior art. In the present invention, the mask comprises the mask liquid and a carrier as described in any one of the present invention, and the mask liquid is attached to the carrier. The present invention does not limit the carrier used in the mask, and any carrier used in the mask in the prior art can be used in the present invention, and no incompatibility phenomenon is found.

[0046] Example

[0047] The present invention is further described in detail below in conjunction with specific examples. It should be understood that the following examples are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0048] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0049] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0050] The examples involve the addition amounts, contents and concentrations of various substances, wherein the percentages described therein, unless otherwise specified, are all by mass percentages.

[0051] Example 1: A polypeptide composition

[0052] A polypeptide composition consists of the following components: palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8, and the mass ratio of each component is 10:1:1:1:1:2.

[0053] Example 2: A polypeptide composition

[0054] A polypeptide composition consists of the following components: palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8, and the mass ratio of the components is 20:1:1:1:1:3.

[0055] Example 3: A polypeptide composition

[0056] A polypeptide composition consists of the following components: palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8, and the mass ratio of each component is 8:1:1:1:1:2.

[0057] Comparative Example 1: A polypeptide composition

[0058] A polypeptide composition consists of the following components: palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, and heptapeptide-6, and the mass ratio of the components is 10:1:1:1:1:1.

[0059] Comparative Example 2: A polypeptide composition

[0060] A polypeptide composition consists of the following components: palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8, and the mass ratio of the components is 1:1:1:1:2.

[0061] Comparative Example 3: A polypeptide composition

[0062] A polypeptide composition consists of the following components: acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8, and the mass ratio of each component is 1:1:1:2.

[0063] Comparative Example 4: A polypeptide composition

[0064] A polypeptide composition consists of the following components: palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, and the mass ratio of each component is 10:1:1:1.

[0065] Comparative Example 5: A polypeptide composition

[0066] A polypeptide composition consists of the following components: palmitoyl tripeptide-8, palmitoyl tripeptide-1, heptapeptide-6 and acetyl hexapeptide-8, and the mass ratio of the components is 10:1:1:2.

[0067] Application Example 1: A facial mask and its preparation method

[0068] A facial mask liquid comprises the polypeptide composition of the present invention, a phase A mixture and a phase B mixture.

[0069] Wherein, the content of the polypeptide composition is 0.00165% by weight of the total weight of the facial mask liquid, and the polypeptide composition is the composition described in Example 1;

[0070] Wherein, by percentage of the total weight of the facial mask liquid, the contents of the ingredients in the phase A are: 4% butylene glycol, 3% propylene glycol, 62% glycerol polyether-2, 2% PEG / PPG-17 / 6 copolymer, 2% betaine, 0.996% erythritol, 0.5% hydroxyethylpiperazineethanesulfonic acid, 0.3% p-hydroxyacetophenone, 0.1% carbomer, 0.1% hydrolyzed sclerotium gum, 0.03% sodium hyaluronate and 74.07% water;

[0071] Wherein, by percentage of the total weight of the facial mask liquid, the contents of the components in the phase B are: taurine 0.589598%, hydroxyethylpiperazine ethane sulfonic acid 0.5502%, azelaic acid MEA 0.4766%, hydrolyzed collagen zinc 0.3724%, potassium methoxysalicylate 0.011%, ellagic acid 0.000138%, grape (VITIS VINIFERA) fruit cell extract 0.000064%, butylene glycol 0.45%, white water lily (NYMPHAEA ALBA) flower extract 0.052%, AHNFELTIOPSIS CONCINNA extract 0.0474%, LITHOSPERMUM OFFICINALE root extract 0.0356%, p-hydroxyacetophenone 0.015%, 1,2-hexanediol 0.54%, Saururus chinensis (SAURURUS CHINENSIS flower extract 0.00044%, purslane (PORTULACA OLERACEA) extract 0.145%, glycerin 0.67%, golden chamomile (CHRYSANTHELLUM INDICUM) extract 0.1%, pelargonium (PELARGONIUM GRAVEOLENS) flower / leaf / stem extract 0.07%, field mint (MENTHA ARVENSIS) leaf extract 0.03%, aloe barbadensis (ALOEBARBADENSIS) extract 0.025%, sophora flavescens (SOPHORA FLAVESCENS) root extract 0.01%, hydroxyethyl urea 0.98%, beta-glucan 0.01%, ethylhexylglycerin 0.00135%, inositol 1%, polysorbate-20 0.02%, panthenol 0.5%, phenoxyethanol 0.0025%, PEG-12 polydimethylsiloxane crosspolymer 0.258%, propylene glycol 0.042%, triethanolamine 0.1% and water 3.63406%.

[0072] The preparation method of the facial mask liquid comprises the following steps:

[0073] (1) Add phase A to the emulsifying pot, turn on the stirrer, stir at 35 rpm, stir and heat to 85°C; turn on the homogenizer, homogenize for 4 minutes, homogenize at 2500 rpm, and keep warm for 30 minutes.

[0074] (2) Turn on the cooling water, cool down to 45°C, add the phase B raw material and the polypeptide composition, stir evenly, and take samples for plate alignment;

[0075] (3) After the plate is qualified, the material is discharged and left to stand, after passing the inspection, it is filled and packaged, and the finished product is inspected and put into storage.

[0076] The mask cloth is immersed in the mask liquid prepared above for 15 minutes, sterilized by microwave, and packaged to obtain a mask containing the polypeptide composition.

[0077] Application Example 2: A facial mask and its preparation method

[0078] The same as Application Example 1, except that the polypeptide composition adopts the composition in Example 2. Application Example 3: A facial mask and its preparation method

[0079] The same as Application Example 1, except that the polypeptide composition adopts the composition in Example 3. Application Example 4: A facial mask and its preparation method

[0080] It is basically the same as Application Example 1, except that the polypeptide composition adopts the composition in Comparative Example 1. Application Example 5: A facial mask and its preparation method

[0081] It is basically the same as Application Example 1, except that the polypeptide composition adopts the composition in Comparative Example 2. Application Example 6: A facial mask and its preparation method

[0082] It is basically the same as Application Example 1, except that the polypeptide composition adopts the composition in Comparative Example 3. Application Example 7: A facial mask and its preparation method

[0083] It is basically the same as Application Example 1, except that the polypeptide composition adopts the composition in Comparative Example 4. Application Example 8: A facial mask and its preparation method

[0084] It is basically the same as Application Example 1, except that the polypeptide composition adopts the composition in Comparative Example 5.

[0085] Test example 1: Product moisturizing test

[0086] Moisturizing test: The skin moisture content is tested using the skin moisture tester MDD4-Corneometer probe CM825 from the German CK company.

[0087] A total of 40 subjects with healthy skin and no history of cosmetic allergies were selected, aged 30-45 years old, divided into 8 groups, 5 people in each group, using the same mask product. Before the test, the subjects wiped their faces with clean water at about 35°C, and then entered the test environment to rest for 30 minutes before starting the test. At 0, 1, 2, 4, and 6 hours after using the mask, the skin moisture content was tested using a skin moisture meter, and the test site was the cheek; 3 measurements were taken and the average value was taken. The larger the value, the greater the moisture content, and vice versa, the smaller the moisture content. The larger the value, the higher the skin moisture content and the better the moisturizing performance.

[0088] The test results are shown in the following table:

[0089]

[0090]

[0091] From the above results, it can be seen that the moisturizing effect of Application Examples 1-3 is significantly higher than that of Application Examples 4-8, indicating that the polypeptide composition of the present invention plays a synergistic moisturizing role in the facial mask.

[0092] Test example 2: Product anti-wrinkle test

[0093] Anti-wrinkle test: The skin elasticity index R7 value was tested using the skin elasticity meter Cotometer MPA580 from German CK company.

[0094] 40 subjects with healthy skin, no history of cosmetic allergies, and obvious forehead wrinkles were selected. They were aged 35-45 years old and divided into 8 groups, 5 people in each group, using the same mask product. The subjects did not use any products on their foreheads within 3 days before the test, and the forehead skin was selected as the test area. The subjects took a measurement before using the mask as the initial value. After the test started, it was used once in the morning and evening every day for a total of four weeks. Take an appropriate amount of mask liquid and apply it evenly on the forehead to ensure that the test area is completely applied and fully absorbed by the mask liquid. The test was performed on the last day of each week, and 3 measurements were taken each time to take the average value. The closer the skin elasticity index R7 is to 1, the better the skin elasticity.

[0095] The calculation formula of the anti-wrinkle rate (%) is as follows, wherein V0 represents the skin elasticity index before using the mask liquid, and V4 represents the skin elasticity index after 4 weeks of use.

[0096] Wrinkle resistance (100%) = (V4-V0) / V0*100%

[0097] The test results are shown in the following table:

[0098]

[0099] From the above results, it can be seen that the anti-wrinkle rates of Application Examples 1-3 are significantly higher than those of Application Examples 4-8, indicating that the polypeptide composition of the present invention plays a synergistic anti-wrinkle role in the facial mask.

[0100] The above-mentioned embodiments only express several implementation methods of the present invention, which is convenient for understanding the technical solution of the present invention in detail, but it cannot be understood as limiting the scope of protection of the invention patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the scope of protection of the present invention.

Claims

1. A polypeptide composition, characterized in that: It includes the following ingredients: Palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8.

2. The polypeptide composition according to claim 1, characterized in that: The mass ratio of palmitoyl tripeptide-8, palmitoyl tripeptide-1, acetyl heptapeptide-4, oligopeptide-1, heptapeptide-6 and acetyl hexapeptide-8 in the polypeptide composition is 10:1:1:1:1:2-20:1:1:1:1:

3.

3. Use of the polypeptide composition according to claim 1 or 2 in the preparation of cosmetics or skin care products, wherein the cosmetics or skin care products are essences, creams, facial cleansers, facial milk, facial masks, eye creams or essence water.

4. The use according to claim 3, characterized in that Measured by mass percentage, the added amount of the polypeptide composition in cosmetics or skin care products is 0.001%-1%.

5. A facial mask liquid, characterized in that: It comprises the polypeptide composition according to claim 1 or 2, a phase A mixture and a phase B mixture, wherein the phase A mixture and the phase B mixture are acceptable excipients and solvents in skin care products.

6. The facial mask liquid according to claim 5, characterized in that, The phase A mixture in the facial mask liquid is butylene glycol, propylene glycol, glyceryl polyether-26, PEG / PPG-17 / 6 copolymer, betaine, erythritol, hydroxyethylpiperazine ethane sulfonic acid, p-hydroxyacetophenone, carbomer, hydrolyzed sclerotium gum, sodium hyaluronate and water; the phase B mixture in the facial mask is taurine, hydroxyethylpiperazine ethane sulfonic acid, azelaic acid MEA, hydrolyzed collagen zinc, potassium methoxysalicylate, ellagic acid, grape (VITISVINIFERA) fruit cell extract, butylene glycol, white water lily (NYMPHAEA ALBA) flower extract, AHNFELTIOPSIS CONCINNA extract, lithospermum officinale (LITHOSPERMUM OFFICINALE) root extract, p-hydroxyacetophenone, 1,2-hexanediol, saururus chinensis (SAURURUS CHINENSIS flower extract, purslane (PORTULACAOLERACEA) extract, glycerin, chrysanthellum indicum extract, pelargonium graveolens flower / leaf / stem extract, mentha arnica leaf extract, aloe barbadensis extract, sophora flavescens root extract, hydroxyethyl urea, beta-glucan, ethylhexylglycerin, inositol, polysorbate 20, panthenol, phenoxyethanol, PEG-12 dimethicone crosspolymer, propylene glycol, triethanolamine and water.

7. The facial mask liquid according to claim 6, characterized in that, Calculated as a percentage of the total weight of the facial mask liquid, the contents of the ingredients in phase A are: 3%-5% butylene glycol, 2%-4% propylene glycol, 1%-3% glycerol polyether-26, 1%-3% PEG / PPG-17 / 6 copolymer, 1%-3% betaine, 0.8%-1.2% erythritol, 0.3%-0.8% hydroxyethylpiperazine ethane sulfonic acid, 0.1%-0.5% p-hydroxyacetophenone, 0.05%-0.2% carbomer, 0.05%-0.15% hydrolyzed sclerotium gum, 0.01%-0.05% sodium hyaluronate and 74%-75% water.

8. The facial mask liquid according to claim 6, characterized in that, The contents of the components in the phase B are as follows: 0.3%-0.7% taurine, 0.3%-0.6% hydroxyethylpiperazine ethane sulfonic acid, 0.2%-0.5% MEA, 0.2%-0.5% hydrolyzed collagen zinc, 0.01%-0.1% potassium methoxysalicylate, 0.0001%-0.1% ellagic acid, 0.00005%-0.1% grape (VITIS VINIFERA) fruit cell extract, 0.3%-0.5% butylene glycol, 0.03%-0.1% NYMPHAEAALBA flower extract, 0.03%-0.1% AHNFELTIOPSISCONCINNA extract, 0.03%-0.1% LITHOSPERMUM OFFICINALE root extract 0.01%-0.1%, parahydroxyacetophenone 0.03%-0.1%, 1,2-hexanediol 0.5%-1%, Saururus CHINENSIS flower extract 0.0003%-0.1%, Portulaca oleracea extract 0.1%-0.3%, glycerin 0.5%-0.8%, Chrysanthellum INDICUM extract 0.05%-0.2%, Pelargonium graveolens flower / leaf / stem extract 0.05%-0.1%, Mentha alvacea leaf extract 0.01%-0.05%, Aloe barbadensis extract 0.02%-0.05%, Sophora flavescens extract FLAVESCENS) root extract 0.01%-0.1%, hydroxyethyl urea 0.5%-1.2%, β-glucan 0.01%-0.05%, ethylhexylglycerin 0.001%-0.1%, inositol 0.5%-1.5%, polysorbate-200.01%-0.05%, panthenol 0.3%-1%, phenoxyethanol 0.002%-0.005%, PEG-12 polydimethylsiloxane crosspolymer 0.1%-0.3%, propylene glycol 0.03%-0.06%, triethanolamine 0.03%-0.2%, water 3-4%.

9. The method for preparing the facial mask liquid according to any one of claims 5 to 8, characterized in that: The method comprises the following steps: (1) Add phase A to an emulsifying pot, turn on the stirrer, stir at a speed of 30-35 rpm, and heat to 85-90°C; turn on the homogenizer, homogenize for 3-5 minutes, at a speed of 2000-3000 rpm, and keep warm for 30 minutes. (2) Turn on the cooling water, cool down to 40-45°C, add the phase B raw material and the polypeptide composition, stir evenly, and take samples for plate alignment; (3) After the plate is qualified, the material is discharged and left to stand, and after passing the inspection, it is filled and packaged, and the finished product is inspected and put into storage.

10. A facial mask, characterized in that: The invention comprises the facial mask liquid as described in any one of claims 5 to 8 and a carrier, wherein the facial mask liquid is attached to the carrier.

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