Quantitative subpackaging device and method for dried blood spots

Abstract

The invention discloses a dry blood spot quantitative subpackaging device and method. The device comprises a base plate, a dried blood paper sheet and an operation switch. The substrate comprises a first surface and a second surface which are arranged back to back in a first direction. The substrate is provided with a groove and an open groove, the groove is arranged on the first surface or the second surface, and the open groove is arranged on the first surface or the second surface. A leading-in channel and a plurality of capillary channels are arranged in the base plate, each capillary channel has a preset volume, and the outlet end of each capillary channel is arranged on the bottom face of the groove. And the operation switch is movably connected in the open slot. The operation switch is used for switching the dry blood spot quantitative subpackaging device to a connection state and a disconnection state during operation. In the communication state, the introduction channel is communicated with the capillary channels. And the blocking state is that the introduction channel is disconnected from the plurality of capillary channels. The dried blood paper sheet is arranged in the groove and covers the outlet end of each capillary channel. The method can realize quantitative detection.

Description

Technical Field

[0001] The present application relates to the field of biochemical detection, and in particular to a device and method for quantitatively packaging dried blood spots. Background Art

[0002] Sampling (such as blood sampling) is the first step in testing human health indicators. Safe, convenient and effective sampling methods can not only protect the subjects from infection and harm during the sampling process, but also improve the accuracy of post-sampling testing. Blood transportation is an important link after blood sampling. Whole blood samples need to be transported by cold chain, which requires an extremely high transportation environment, increases transportation costs, and is difficult to maintain long-term stability.

[0003] Dried blood spot (DBS) technology is to collect whole blood samples on cardboard to form dried blood spots, and then send the cardboard for inspection. The above method requires a small amount of blood. As long as a few microliters to tens of microliters of blood are formed on the cardboard to form a circular dried blood spot of appropriate size, it can meet the inspection needs. Infectious pathogens (such as HIV, hepatitis B virus, etc.) cannot survive in the dried blood spot environment, and the test object can remain stable in the dried blood spot environment, without the need for cold chain transportation.

[0004] However, the collection of dried blood spots is usually non-quantitative, which limits the application of dried blood spot technology in some situations where quantitative testing is required (such as screening for metabolic diseases in newborns). Summary of the invention

[0005] In order to solve the above shortcomings, it is necessary to provide a dry blood spot quantitative packaging device.

[0006] In addition, it is also necessary to provide a dry blood spot quantitative packaging method applied to the above-mentioned dry blood spot quantitative packaging device.

[0007] In a first aspect, the present application provides a dry blood spot quantitative dispensing device, comprising a substrate, an operating switch and a dry blood paper. The substrate comprises a first surface and a second surface disposed opposite to each other in a first direction, and the substrate is provided with a groove and a slot, each of the groove and the slot being independently provided on the first surface or the second surface. An introduction channel and a plurality of capillary channels are provided in the substrate, the introduction channel being used to introduce a blood sample, each of the capillary channels having a predetermined volume, and the outlet end of each of the capillary channels being provided on the bottom surface of the groove. The operating switch is movably connected in the slot, and the operating switch is used to switch the dry blood spot quantitative dispensing device to a connected state and a blocked state respectively during operation. In the connected state, the introduction channel is connected to a plurality of the capillary channels, so that the blood sample introduced by the introduction channel enters each of the capillary channels, and fills each of the capillary channels under capillary action and extends to the outlet end of the capillary channels. The blocked state is that the introduction channel and the plurality of the capillary channels are disconnected. The dried blood paper sheet is arranged in the groove and covers the outlet end of each capillary channel. The dried blood paper sheet is used to absorb the blood sample in the capillary channel through the outlet end of each capillary channel and form a dried blood spot.

[0008] In some possible implementations, the operation switch is rotatably disposed in the slot, an intermediate channel is disposed in the operation switch, and the operation switch is used to switch the dry blood spot quantitative dispensing device to the connected state and the blocked state when rotating. The connected state is that the intermediate channel is connected to the introduction channel and the plurality of capillary channels respectively, and the blocked state is that the intermediate channel is disconnected from the introduction channel and the plurality of capillary channels respectively.

[0009] In some possible implementations, the operation switch is slidably disposed in the slot along the first direction, and an intermediate channel is disposed in the operation switch. The operation switch is used to switch the dry blood spot quantitative dispensing device to the connected state and the blocked state when sliding along the first direction. The connected state is that the intermediate channel is connected to the introduction channel and the plurality of capillary channels respectively, and the blocked state is that the intermediate channel is disconnected from the introduction channel and the plurality of capillary channels respectively.

[0010] In some possible implementations, the dry blood spot quantitative dispensing device further includes a sealing member, which is disposed around the operating switch. When the dry blood spot quantitative dispensing device is in the connected state, the sealing member is staggered with the outlet end of the introduction channel and the inlet end of each of the capillary channels. When the dry blood spot quantitative dispensing device is in the blocked state, the sealing member is sealed and connected to the outlet end of the introduction channel and the inlet end of each of the capillary channels.

[0011] In some possible implementations, the dry blood spot quantitative packaging device further includes a buffer member, and the buffer member is disposed between the operating switch and the bottom surface of the groove in the first direction.

[0012] In some possible implementations, the operating switch is slidably disposed in the slot along a second direction perpendicular to the first direction. The outlet end of the introduction channel is connected to one end of the slot. The operating switch is used to switch the dry blood spot quantitative packaging device to the connected state and the blocked state when sliding along the second direction. The connected state is that a negative pressure is generated in the space between the end of the slot and the operating switch, and the introduction channel is connected to the multiple capillary channels through the space. The blocked state is that the operating switch isolates the end and the multiple capillary channels, so that the introduction channel and the multiple capillary channels are disconnected.

[0013] In some possible implementations, the operating switch includes a main body and an operating part. The main body is disposed in the slot. The main body includes a first sealing part and a second sealing part disposed opposite to each other in the second direction, and a connecting part connected between the first sealing part and the second sealing part. The operating part is connected to the connecting part and convexly disposed in the slot. The first sealing part and the second sealing part are both sealed and connected in the slot.

[0014] In some possible implementations, the substrate further includes a side surface connected between the first surface and the second surface, and the inlet end of the introduction channel is disposed on the side surface. The dry blood spot quantitative packaging device further includes a blood collector detachably mounted on the side surface and a hollow puncture portion disposed in the introduction channel, the puncture portion protruding from the inlet end of the introduction channel and extending into the blood collector.

[0015] In some possible implementations, the substrate further includes a side surface connected between the first surface and the second surface, and the inlet end of the introduction channel is arranged on the side surface. The dry blood spot quantitative packaging device further includes a blood collector integrally mounted on the side surface, negative pressure is formed in the blood collector, and the inlet end of the introduction channel is connected to the blood collector.

[0016] In some possible implementations, a plurality of blood spot sampling points are provided on the dried blood paper sheet, the outlet ends of the plurality of capillary channels are arranged along the same straight line and are directly opposite to the blood spot sampling points, the volume of each capillary channel corresponds to the size of the corresponding dried blood spot sampling point, and at least one capillary channel is provided with a bending section.

[0017] In some possible implementations, the substrate is made of transparent plastic material.

[0018] The second aspect of the present application also provides a dry blood spot quantitative packaging method applied to the above-mentioned dry blood spot quantitative packaging device, comprising: operating the operation switch to switch the dry blood spot quantitative packaging device to a connected state, so that the introduction channel is connected to multiple capillary channels; introducing a blood sample through the introduction channel, so that the blood sample fills each capillary channel under capillary action and extends to the outlet end of the capillary channel; operating the operation switch to switch the dry blood spot quantitative packaging device to a blocked state, so that the introduction channel and multiple capillary channels are disconnected; and adsorbing the blood sample in the capillary channel through the suction force of the dry blood paper sheet, so that the blood sample flows to the dry blood paper sheet through the outlet end of the capillary channel and forms a dry blood spot.

[0019] In the dry blood spot quantitative packaging device of the present application, the capillary channel is set to a predetermined volume according to actual needs, and the operating switch is operated to put the dry blood spot quantitative packaging device in a connected state. At this time, the blood sample flowing in through the inlet end of the introduction channel fills each capillary channel under capillary action and extends to the outlet end of the capillary channel. At this time, the blood is quantified in the capillary channel; then the operating switch is operated to put the dry blood spot quantitative packaging device in a blocked state, disconnecting the blood source, and the blood in the capillary channel is printed onto the dry blood paper sheet through the outlet end of the capillary channel under the suction of the dry blood paper sheet to form a dry blood spot, thereby completing the quantitative collection of the blood sample with high quantitative accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the three-dimensional structure of a dry blood spot quantitative packaging device provided in one embodiment of the present application.

[0021] Figure 2 for Figure 1 A schematic diagram of the three-dimensional structure of the dry blood spot quantitative packaging device from another angle is shown.

[0022] Figure 3 for Figure 1 An exploded view of the dried blood spot quantitative dispensing device is shown.

[0023] Figure 4 for Figure 1 The cross-sectional view of the dried blood spot quantitative dispensing device along IV-IV is shown.

[0024] Figure 5 for Figure 4 The cross-sectional view of the dry blood spot quantitative dispensing device shown is after the operating switch is turned.

[0025] Figure 6 for Figure 4 The cross-sectional view of the dry blood spot quantitative dispensing device shown is after the operating switch is pressed.

[0026] Figure 7 for Figure 1 The dry blood spot quantitative dispensing device shown is a top view with the first cover of the base plate removed.

[0027] Figure 8 for Figure 7 The illustrated diagram is a top view of the dry blood spot quantitative dispensing device after the operating switch is turned.

[0028] Fig. 9 This is a schematic diagram of the three-dimensional structure of a dry blood spot quantitative packaging device provided in another embodiment of the present application.

[0029] Fig.10 for Fig. 9 An exploded view of the dried blood spot quantitative dispensing device is shown.

[0030] Fig.11 for Fig. 9 The cross-sectional view of the dry blood spot quantitative dispensing device along line XI-XI is shown.

[0031] Fig.12 for Fig.11 The cross-sectional view of the dry blood spot quantitative dispensing device is shown after the operating switch is slid.

[0032] Fig.13 This is a schematic diagram of the three-dimensional structure of a dry blood spot quantitative packaging device provided in yet another embodiment of the present application.

[0033] Fig.14 for Fig.13 The cross-sectional view of the dried blood spot quantitative dispensing device along line XIV-XIV is shown.

[0034] Fig.15 for Fig.14 The cross-sectional view of the dry blood spot quantitative dispensing device is shown after the operating switch is slid.

[0035] Fig.16 This is a flow chart of a method for quantitative packaging of dried blood spots provided in one embodiment of the present application.

[0036] Main component symbols

[0037] Substrate 10

[0038] First cover 10a

[0039] Second cover 10b

[0040] First surface 11

[0041] Second surface 12

[0042] Side 13

[0043] Introducing channel 14

[0044] Capillary channel 15

[0045] Dried blood paper 20

[0046] Blood spot sampling point 21

[0047] Operation switch 30

[0048] Middle Channel 31

[0049] Main body 32

[0050] The third cover 32a

[0051] Fourth cover 32b

[0052] First sealing portion 32c

[0053] Second sealing portion 32d

[0054] Connecting portion 32e

[0055] Operation unit 33

[0056] Seal 40

[0057] Buffer 50 Dried blood spot quantitative packaging device 100, 200, 300 Slotting 110

[0058] Groove 120 Bottom 111, 121

[0059] First Area 131

[0060] Second area 132

[0061] Third Area 133

[0062] Fourth Area 134

[0063] Fifth Region 135

[0064] Area 6 136

[0065] First inlet port 141

[0066] The first outlet port 142

[0067] Acupuncture 143

[0068] Blood collector 144

[0069] The third inlet port 151

[0070] The third outlet port 152

[0071] The second inlet port 311

[0072] Second outlet port 312

[0073] Opening 1100

[0074] First opening section 1100a

[0075] The second opening section 1100b

[0076] The third opening section 1100c

[0077] First direction X

[0078] Second direction Y

[0079] The third direction Z

[0080] The following specific implementation methods will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0081] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments.

[0082] It should be noted that when a component is considered to be "set on" another component, it can be directly set on the other component or there can be a central component at the same time; when a component is considered to be "installed on" another component, it can be directly installed on the other component or there can be a central component at the same time. The term "and / or" used herein includes all and any combinations of one or more related listed items.

[0083] See also Figures 1 to 3 In one embodiment of the present application, a dry blood spot quantitative packaging device 100 is provided, which can be applied to the screening of neonatal metabolic diseases, the collection of infectious disease samples, the detection of doping in athletes, the detection of health markers (Biomarker), and other occasions requiring quantitative detection. The dry blood spot quantitative packaging device 100 includes a substrate 10, a dry blood paper sheet 20, and an operation switch 30.

[0084] The substrate 10 includes a first surface 11, a second surface 12, and a side surface 13. The first surface 11 and the second surface 12 are arranged opposite to each other in a first direction X. The side surface 13 is connected between the first surface 11 and the second surface 12. A portion of the second surface 12 is recessed toward the first surface 11 to form a groove 120. The groove 120 does not penetrate the first surface 11, that is, the bottom surface 121 (at Figure 4 ) is disposed between the first surface 11 and the second surface 12 in the first direction X. The dried blood paper 20 is disposed in the groove 120. Figure 3 As shown, a plurality of blood spot sampling points 21 are provided on the dried blood paper sheet 20. In other embodiments, the groove 120 may also be provided on the first surface 11.

[0085] Please refer to Figure 4 and Figure 7 , an introduction channel 14 and a plurality of capillary channels 15 that can be connected to the introduction channel 14 are provided in the substrate 10. The introduction channel 14 includes a first inlet end 141 and a first outlet end 142 that are oppositely arranged, and the first inlet end 141 is opened on the side 13. In some embodiments, the substrate 10 includes a first cover body 10a and a second cover body 10b stacked in a first direction X. The first cover body 10a includes a first surface 11, and the second cover body 10b includes a second surface 12. The introduction channel 14 and the capillary channel 15 can both be opened on a side of the second cover body 10b facing the first cover body 10a. The first cover body 10a covers the second cover body 10b, thereby closing the introduction channel 14 and the capillary channel 15 in the first direction X. Further, in some embodiments, the groove 120 does not penetrate the second cover body 10b. As Figure 1 and Figure 3 As shown, in some embodiments, the side 13 includes a first area 131, a second area 132, a third area 133, a fourth area 134, a fifth area 135 and a sixth area 136 connected end to end. The third area 133 and the fifth area 135 are arranged opposite to each other in a second direction Y perpendicular to the first direction X, and the third area 133 may be parallel to the fifth area 135. The fourth area 134 is vertically connected between the third area 133 and the fifth area 135, and the first area 131 may be parallel to the fourth area 134. The second area 132 and the sixth area 136 may be obliquely connected to the first area 131, respectively. In the second direction Y, the size of the first area 131 is smaller than that of the fourth area 134. The first inlet end 141 of the introduction channel 14 is opened in the first area 131.

[0086] like Figure 3 and Figure 4 As shown, part of the first surface 11 is recessed toward the second surface 12 to form a groove 110. The groove 110 does not penetrate the second surface 12. The operating switch 30 is movably connected in the groove 110 of the substrate 10. In a third direction Z perpendicular to both the first direction X and the second direction Y, the operating switch 30 is disposed between the introduction channel 14 and each capillary channel 15. More specifically, in the third direction Z, the operating switch 30 is disposed between the first outlet end 142 of the introduction channel 14 and each capillary channel 15. An intermediate channel 31 is disposed in the operating switch 30. The intermediate channel 31 includes a second inlet end 311 and a plurality of second outlet ends 312. As shown in FIG. Figure 4 , Figure 5 , Figure 7 and Figure 8As shown, the operating switch 30 is used to switch the dry blood spot quantitative dispensing device 100 to a connected state and a blocked state respectively during operation. Figure 4 and Figure 7 As shown, the connected state is that the second inlet end 311 is connected to the first outlet end 142 of the introduction channel 14, and the plurality of second outlet ends 312 are respectively connected to the plurality of capillary channels 15. Therefore, when the dry blood spot quantitative dispensing device 100 is in the connected state, the blood sample introduced through the introduction channel 14 can enter the plurality of capillary channels 15 through the intermediate channel 31. Figure 5 and Figure 8 As shown, the blocking state is that the second inlet end 311 is disconnected from the introduction channel 14, and the plurality of second outlet ends 312 are respectively disconnected from the plurality of capillary channels 15. Therefore, when the dry blood spot quantitative dispensing device 100 is in the blocking state, the blood sample introduced through the introduction channel 14 is blocked by the operating switch 30, preventing the blood sample from entering the plurality of capillary channels 15 through the intermediate channel 31. In other embodiments, the groove 110 may also be provided on the second surface 12.

[0087] In some embodiments, the operating switch 30 is rotatably disposed in the slot 110, and the operating switch 30 switches the dry blood spot quantitative dispensing device 100 to a connected state and a blocked state when rotating. Figure 7 and Figure 8 As shown, when the dry blood spot quantitative dispensing device 100 is switched from the connected state to the blocked state, the rotation angle of the operating switch 30 can be approximately 90 degrees. Figure 4 and Figure 5 In other embodiments, the operating switch 30 may also be slidably disposed in the slot 110 along the first direction X. In this case, the operating switch 30 may also be slid along the first direction X (e.g., the operating switch 30 may be pressed), and the second inlet end 311 may also be disconnected from the introduction channel 14. Figure 3 and Figure 4 As shown, the operating switch 30 includes a main body 32 and an operating part 33 connected in a first direction X. The main body 32 is arranged in the substrate 10, and the intermediate channel 31 is arranged in the main body 32. The operating part 33 is convexly arranged on the first surface 11 of the substrate 10, and the operator can rotate or press the operating switch 30 by operating the operating part 33. Further, in some embodiments, the main body 32 may include a third cover 32a and a fourth cover 32b stacked in the first direction X, and the operating part 33 is connected to the third cover 32a. Multiple capillary channels 15 can be arranged on the side of the fourth cover 32b facing the third cover 32a. The third cover 32a covers the fourth cover 32b, thereby closing multiple capillary channels 15 in the first direction X.

[0088] Each capillary channel 15 is set to a predetermined volume according to actual needs, so as to facilitate quantitative blood sampling. The volumes of the capillary channels 15 can be the same or different. Figure 4 , Figure 5 , Figure 7 and Figure 8 As shown, each capillary channel 15 includes a third inlet end 151 and a third outlet end 152 that are arranged opposite to each other. Each third inlet end 151 is used to communicate with one of the second outlet ends 312 of the middle channel 31. The third outlet end 152 is opened on the bottom surface 121 of the groove 120, and the dried blood paper sheet 20 covers the third outlet end 152. During use, the operator can operate the operating switch 30 to put the dry blood spot quantitative dispensing device 100 in a connected state. At this time, the blood sample flowing in through the first inlet end 141 of the introduction channel 14 flows through the middle channel 31, and fills each capillary channel 15 under capillary action and extends to the third outlet end 152. At this time, the blood sample is quantitatively collected in the capillary channel 15. It can be understood that due to the tension of the blood sample itself, the blood sample will not flow out through the third outlet end 152 of the capillary channel 15; then the operating switch 30 is operated to put the dry blood spot quantitative dispensing device 100 in a blocked state, disconnecting the blood source, and the blood sample in the capillary channel 15 is printed onto the dry blood paper sheet 20 through the third outlet end 152 under the suction of the dry blood paper sheet 20 to form a circular dry blood spot. At this time, blood sampling is completed. In other embodiments, a hydrolyzed gel (not shown) may also be provided at the third outlet end 152 of the capillary channel 15 . After the dry blood spot quantitative packaging device 100 is in a connected state and the blood sample fills the capillary channel 15 , the hydrolyzed gel may prevent the blood sample from flowing out directly from the third outlet end 152 .

[0089] In some embodiments, the substrate 10 may be a transparent plastic plate, which is convenient for the operator to observe whether the blood sample fills each capillary channel 15, so that when the blood sample fills each capillary channel 15, the operating switch 30 is operated to put the dry blood spot quantitative packaging device 100 into a blocked state. In other embodiments, the operating switch 30 may be operated to put the dry blood spot quantitative packaging device 100 into a blocked state when the blood sample just begins to appear on the dry blood paper 20. Personal information such as the blood sampling subject and the blood sampling time may be recorded on the dry blood paper 20. In some embodiments, the third outlet ends 152 of each capillary channel 15 may be arranged along the same straight line and directly opposite to the blood spot sampling point 21, and the volume of each capillary channel 15 corresponds to the size of the corresponding dry blood spot sampling point 21. Figure 7 As shown, at least one capillary channel 15 may be provided with a bending section, so that the inner space of the substrate 10 can be fully utilized while achieving the required volume.

[0090] In some embodiments, a hollow puncture portion (not shown) may be provided in the introduction channel 14, and the puncture portion may protrude from the first inlet end 141 of the introduction channel 14. The puncture portion is used to puncture a blood collector (such as a negative pressure tube for collecting blood samples), so that the blood sample in the blood collector flows into the introduction channel 14. When in use, the dry blood spot quantitative dispensing device 100 can be placed vertically, that is, the first inlet end 141 of the introduction channel 14 faces upward; then the blood collector is placed above the first inlet end 141, and the blood collector is punctured by the puncture portion, so that the blood sample in the blood collector flows into the introduction channel 14 under the action of gravity. Since the size of the fourth area 134 in the second direction Y is large, the stability of the dry blood spot quantitative dispensing device 100 is improved when it is placed vertically. In some embodiments, the puncture portion can be a hollow needle body made of metal, and the diameter of the needle body is 0.1 mm to 2 mm.

[0091] like Figure 3 and Figure 4 As shown, in some embodiments, the dry blood spot quantitative dispensing device 100 may further include a sealing member 40, which may be disposed around the switch portion of the operating switch 30. When the dry blood spot quantitative dispensing device 100 is in a connected state, the sealing member 40 is staggered with the first outlet end 142 of the introduction channel 14 and the third inlet end 151 of each capillary channel 15. When the dry blood spot quantitative dispensing device 100 is in a blocked state, the sealing member 40 is sealed and connected to the first outlet end 142 of the introduction channel 14 and the third inlet end 151 of each capillary channel 15, thereby preventing the blood sample in the introduction channel 14 from overflowing through the first outlet end 142 and preventing the blood sample in each capillary channel 15 from overflowing through the third inlet end 151. In some embodiments, the material of the sealing member 40 may be silicone.

[0092] The dry blood spot quantitative dispensing device 100 may further include a buffer 50, which is disposed between the operating switch 30 and the bottom surface 111 of the slot 110 in the first direction X. The buffer 50 may play a buffering role when the operating switch 30 is rotated, thereby preventing the operating switch 30 from being worn due to frequent rotation. In addition, when the operating switch 30 is configured to be pressed along the first direction X so that the second inlet end 311 is disconnected from the introduction channel 14, the buffer 50 may also be elastically compressed when the operating switch 30 is pressed, thereby providing space for the displacement of the operating switch 30 in the first direction X. In some embodiments, the material of the second sealing member 40 may be silicone.

[0093] See also Figures 9 to 12Another embodiment of the present application also provides a dry blood spot quantitative dispensing device 200. The difference from the above-mentioned dry blood spot quantitative dispensing device 100 is that no intermediate channel 31 is provided in the operating switch 30. In some embodiments, the operating switch 31 can be slidably disposed in the slot 110 along the second direction Y, and the first outlet end 142 of the introduction channel 14 is connected to one end of the slot 110. In the second direction Y, the length of the operating switch 31 is less than the length of the slot 110. The operating switch 31 is used to switch the dry blood spot quantitative dispensing device 200 to a connected state and a blocked state when sliding.

[0094] The operating switch 30 includes a main body 32 and an operating part 33 connected to each other. The main body 32 is disposed in the substrate 10 and extends along the second direction Y. The operating part 33 is convexly disposed on the first surface 11 of the substrate 10, and the operator can slide the operating switch 30 by operating the operating part 33. Further, in some embodiments, the main body 32 may include a first sealing part 32c, a second sealing part 32d disposed opposite to the first sealing part 32c in the second direction Y, and a connecting part 32e connected between the first sealing part 32c and the second sealing part 32d. In the third direction Y, the first sealing part 32c is closer to the first outlet end 142 than the second sealing part 32d. The operating part 33 is disposed on the connecting part 32e. The first sealing part 32c and the second sealing part 32d are both sealed and connected in the groove 110.

[0095] For example, the first sealing portion 32c and the second sealing portion 32d may be made of an elastic material (such as rubber). In the third direction Z, the original width of the first sealing portion 32c (i.e., the width when the first sealing portion 32c is not assembled in the slot 110) may be greater than the width of the slot 110, and the original width of the second sealing portion 32d (i.e., the width when the second sealing portion 32d is not assembled in the slot 110) may be greater than the width of the slot 110, so that an interference fit is formed between the first sealing portion 32c and the second sealing portion 32d and the slot 110.

[0096] Therefore, if Fig.11 As shown, when the operating switch 30 is located at the end portion connected to the first outlet end 142 of the slot 110, the first sealing portion 32c can isolate the first outlet end 142 and the capillary channel 15 in the third direction X. At this time, the dry blood spot quantitative packaging device 200 is in a blocking state, preventing the blood sample from entering the multiple capillary channels 15 through the intermediate channel 31. Fig.12As shown, when the operating switch 30 slides to the other end of the slot 110 away from the first outlet end 14, the slot 110 generates negative pressure in the space between the end connected to the first outlet end 142 and the first sealing portion 32c, and the first sealing portion 32c does not isolate the first outlet end 142 and the capillary channel 15 in the third direction X. At this time, the dry blood spot quantitative dispensing device 200 is in a connected state, so that the blood sample introduced through the introduction channel 14 can enter the slot 110 and the multiple capillary channels 15 in sequence under the negative pressure in the slot 110. Due to the arrangement of the first sealing portion 32c and the second sealing portion 32d in the operating switch 30, the dry blood spot quantitative dispensing device 200 has a better sealing and anti-leakage effect when it is in a connected state, and improves the situation that the blood sample introduced through the introduction channel 14 leaks between the first outlet end 142 and the second inlet end 311 or between the second outlet end 312 and the third inlet end 151.

[0097] like Fig. 9 and Fig.10 As shown, the slot 110 forms an opening 1100 on the first surface 11, and the operating portion 33 of the operating switch 31 is protruded from the first surface 11 via the opening 1100. The opening 1100 includes a first opening section 1100a, a second opening section 1100b, and a third opening section 1100c connected in sequence. The first opening section 1100a and the third opening section 1100c can both be arranged along the second direction Y, and the second opening section 1100b can be arranged along the third direction Z. When the operating portion 33 slides from the third opening section 1100c to the first opening section 1100a, the dry blood spot quantitative dispensing and packaging device 200 is switched from the connected state to the blocked state.

[0098] In some embodiments, a blood collector 144 (such as a negative pressure tube for collecting blood samples) is detachably mounted on the side 13 of the substrate 10 . When the blood collector 144 is mounted on the side 13 , the blood sample in the blood collector 144 flows into the introduction channel 14 via the puncture portion 143 .

[0099] See also Figures 13 to 15 Another embodiment of the present application also provides a dry blood spot quantitative packaging device 300. The difference from the above-mentioned dry blood spot quantitative packaging device 200 is that the blood collector 144 is integrally formed on the side 13. In this embodiment, the blood collector 144 can be installed on another blood collection device (not shown) when in use. Under the action of the negative pressure in the blood collector 144, the blood sample in the blood collection device can first flow into the blood collector 144, and then by operating the operating switch 30, the blood sample in the blood collector 144 can be controlled to flow into the capillary channel 15 through the introduction channel 14.

[0100] See also Fig.16The present application also provides a method for quantitatively packaging dried blood spots applied to the device for quantitatively packaging dried blood spots 100 (or the devices for quantitatively packaging dried blood spots 200, 300), comprising the following steps:

[0101] Step S1 , operating the operating switch 30 to switch the dry blood spot quantitative dispensing device 100 to a connected state, so that the introduction channel 14 is connected to the plurality of capillary channels 15 .

[0102] Step S2 , introducing a blood sample through the introduction channel 14 , so that the blood sample fills each capillary channel 15 under capillary action and extends to the outlet end of the capillary channel 15 .

[0103] Step S3, operating the operating switch 30 to switch the dry blood spot quantitative dispensing device 100 to a blocking state, thereby disconnecting the introduction channel 14 and the plurality of capillary channels 15.

[0104] Step S4, the blood sample in the capillary channel 15 is adsorbed by the suction force of the dried blood paper 20, so that the blood sample flows to the dried blood paper 20 through the outlet end of the capillary channel 15 and forms a dried blood spot.

[0105] In the dry blood spot quantitative packaging device 100 of the present application, the capillary channel 15 is set to a predetermined volume according to actual needs, and the operating switch 30 is operated to put the dry blood spot quantitative packaging device 100 in a connected state. At this time, the blood sample flowing in through the first inlet end 141 of the introduction channel 14 fills each capillary channel 15 under capillary action and extends to the third outlet end 152. At this time, the blood is quantified in the capillary channel 15; then the operating switch 30 is operated to put the dry blood spot quantitative packaging device 100 in a blocked state, disconnecting the blood source, and the blood in the capillary channel 15 is printed onto the dry blood paper sheet 20 through the third outlet end 152 under the suction of the dry blood paper sheet 20 to form a circular dry blood spot, thereby completing the quantitative collection of the blood sample with high quantitative accuracy.

[0106] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present application and are not intended to limit it. Although the present application has been described in detail with reference to the preferred embodiments, a person of ordinary skill in the art should understand that the technical solution of the present application may be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present application.

Claims

1. A dry blood spot quantitative packaging device, characterized in that: include: A substrate, comprising a first surface and a second surface disposed opposite to each other in a first direction, the substrate being provided with a groove and a slot, each of the groove and the slot being independently provided on the first surface or the second surface; an introduction channel and a plurality of capillary channels being provided in the substrate, the introduction channel being used to introduce a blood sample, each of the capillary channels having a predetermined volume, and an outlet end of each of the capillary channels being provided on the bottom surface of the groove; an operating switch, movably connected in the slot, the operating switch being used to switch the dry blood spot quantitative dispensing device to a connected state and a blocked state respectively during operation, wherein the connected state is that the introduction channel is connected to the plurality of capillary channels, so that the blood sample introduced by the introduction channel enters each of the capillary channels and fills each of the capillary channels under capillary action and extends to the outlet end of the capillary channels, and the blocked state is that the introduction channel is disconnected from the plurality of capillary channels; as well as A dried blood paper sheet is arranged in the groove and covers the outlet end of each capillary channel. The dried blood paper sheet is used to absorb the blood sample in the capillary channel through the outlet end of each capillary channel and form a dried blood spot.

2. The dry blood spot quantitative packaging device according to claim 1, characterized in that: The operating switch is rotatably disposed in the slot, and an intermediate channel is disposed in the operating switch. The operating switch is used to switch the dry blood spot quantitative packaging device to the connected state and the blocked state when rotating. The connected state is that the intermediate channel is connected to the introduction channel and the plurality of capillary channels respectively, and the blocked state is that the intermediate channel is disconnected from the introduction channel and the plurality of capillary channels respectively.

3. The dry blood spot quantitative packaging device according to claim 1, characterized in that: The operating switch is slidably disposed in the groove along the first direction, and an intermediate channel is disposed in the operating switch. The operating switch is used to switch the dry blood spot quantitative packaging device to the connected state and the blocked state when sliding along the first direction. The connected state is that the intermediate channel is respectively connected to the introduction channel and the multiple capillary channels, and the blocked state is that the intermediate channel is respectively disconnected from the introduction channel and the multiple capillary channels.

4. The dry blood spot quantitative packaging device according to claim 2 or 3, characterized in that: The dry blood spot quantitative packaging device also includes a sealing member, which is arranged around the operating switch. When the dry blood spot quantitative packaging device is in the connected state, the sealing member is staggered with the outlet end of the introduction channel and the inlet end of each of the capillary channels, respectively. When the dry blood spot quantitative packaging device is in the blocked state, the sealing member is sealed and connected to the outlet end of the introduction channel and the inlet end of each of the capillary channels.

5. The dry blood spot quantitative packaging device according to claim 2 or 3, characterized in that: The dry blood spot quantitative packaging device further comprises a buffer member, which is arranged between the operating switch and the bottom surface of the groove in the first direction.

6. The dry blood spot quantitative packaging device according to claim 1, characterized in that: The operating switch is slidably disposed in the slot along a second direction perpendicular to the first direction, and the outlet end of the introduction channel is connected to one end of the slot. The operating switch is used to switch the dry blood spot quantitative packaging device to the connected state and the blocked state when sliding along the second direction. The connected state is that negative pressure is generated in the space between the end of the slot and the operating switch, and the introduction channel is connected to the multiple capillary channels through the space. The blocked state is that the operating switch isolates the end and the multiple capillary channels, so that the introduction channel and the multiple capillary channels are disconnected.

7. The dry blood spot quantitative packaging device according to claim 6, characterized in that: The operating switch includes a main body and an operating part, the main body is arranged in the groove, the main body includes a first sealing part and a second sealing part arranged opposite to each other in the second direction, and a connecting part connected between the first sealing part and the second sealing part, the operating part is connected to the connecting part and protrudes from the groove; the first sealing part and the second sealing part are both sealed and connected in the groove.

8. The dry blood spot quantitative packaging device according to claim 6, characterized in that: The substrate also includes a side surface connected between the first surface and the second surface, the inlet end of the introduction channel is arranged on the side surface, and the dry blood spot quantitative packaging device also includes a blood collector detachably mounted on the side surface and a hollow pricking portion arranged in the introduction channel, the pricking portion protrudes from the inlet end of the introduction channel and extends into the blood collector.

9. The dry blood spot quantitative packaging device according to claim 6, characterized in that: The substrate also includes a side surface connected between the first surface and the second surface, and the inlet end of the introduction channel is arranged on the side surface. The dry blood spot quantitative packaging device also includes a blood collector integrally installed on the side surface, and a negative pressure is formed in the blood collector. The inlet end of the introduction channel is connected to the blood collector.

10. The dry blood spot quantitative packaging device according to claim 1, characterized in that: A plurality of blood spot sampling points are arranged on the dried blood paper sheet, and the outlet ends of the plurality of capillary channels are arranged along the same straight line and directly opposite to the blood spot sampling points. The volume of each capillary channel corresponds to the size of the corresponding dried blood spot sampling point, and at least one capillary channel is provided with a bending section.

11. The dry blood spot quantitative packaging device according to claim 1, characterized in that: The substrate is made of transparent plastic material.

12. A method for quantitatively dispensing dried blood spots applied to the device for quantitatively dispensing dried blood spots according to any one of claims 1 to 11, characterized in that: include: Operating the operation switch to switch the dry blood spot quantitative dispensing device to a connected state, so that the introduction channel is connected to the plurality of capillary channels; Introducing a blood sample through the introduction channel, so that the blood sample fills each of the capillary channels under capillary action and extends to the outlet end of the capillary channel; Operating the operation switch to switch the dry blood spot quantitative dispensing device to a blocking state, thereby disconnecting the introduction channel and the plurality of capillary channels; as well as The blood sample in the capillary channel is adsorbed by the suction force of the dried blood paper sheet, so that the blood sample flows to the dried blood paper sheet through the outlet end of the capillary channel and forms a dried blood spot.

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