Biosensing device based on nano composite material and use method of biosensing device

Abstract

The invention relates to the technical field of biosensing devices, and provides a biosensing device based on a nano composite material and a use method thereof, the characteristics of the nano material are utilized to form a composite carrier of a biological detection body so as to achieve the purposes of optimizing detection data and improving detection reliability and sensitivity, the detection range is wide, the practicability is good, and the application range is wide. Comprising a sensor and a detection carrier, an operation cavity is formed in the sensor, the detection carrier comprises a fixed column and a rotating sleeve, the fixed column is fixedly connected into the operation cavity, a rotating connection limiting assembly is installed on the fixed column, the rotating sleeve is installed on the rotating connection limiting assembly, the fixed column is sleeved with the rotating sleeve, and a plurality of branch pipes are fixedly connected to the outside of the rotating sleeve; the plurality of branch pipes are fixedly connected with carrier boxes, the plurality of carrier boxes are internally provided with nano composite material films, the plurality of nano composite material films are fixedly loaded with biological detection bodies, the plurality of biological detection bodies are different biological detection bodies, and the plurality of carrier boxes are internally provided with electronic detection parts.

Description

Technical Field

[0001] The present invention relates to the technical field of biosensor devices, and in particular to a biosensor device based on nanocomposite materials and a method for using the same. Background Art

[0002] As the name suggests, a biosensor is a device that combines biological components with physical and chemical detectors. It is an analytical tool or system composed of immobilized biosensitive materials as recognition elements (including enzymes, antibodies, antigens, microorganisms, cells, tissues, nucleic acids and other biologically active substances), appropriate physical and chemical transducers (such as oxygen electrodes, photosensitive tubes, field effect transistors, piezoelectric crystals, etc.) and signal amplification devices. Its purpose is to convert the properties of the analyte such as type and concentration into quantitative data that are easily accepted by people through a series of reactions for easy analysis.

[0003] After searching, the invention patent with Chinese patent application number CN201410649144.X discloses a biosensor, which is roughly described as including a test paper that can be inserted, which has a reaction area and a plurality of electrodes, the reaction area is used to place a reactant, and the biosensor includes: a sensor, which is connected to the test paper electrode, the sensor generates an output signal according to the reactant in the reaction area, a data card, which has built-in parameters and operation program data of the test paper, and the data card has a detection contact, a body, which has a first slot and a second slot, the first slot is for the test paper to be inserted, and the second slot is for the data card to be inserted, and a controller is arranged inside the body and connected to the first slot and the second slot, and has a built-in memory for storing data. When the data card is inserted into the first slot, the data card is stored in the memory. After the second slot is inserted, the controller will detect the detection contact, that is, immediately detect the existence of the data card, and quickly read the test paper parameters and the operation program data on the data card once and store them in the memory. After that, the data card can be removed from the second slot. The controller will calculate the corresponding output signal generated by the sensor when the reactant is in the reaction area of ​​the test paper according to the parameters and operation program data stored in the memory, and calculate the concentration value result of the reactant, and store the calculated concentration value result in the memory. When in use, when the controller detects the detection contact, it can quickly store the parameters and operation program of the built-in test paper on the data card in the controller, and it can also enable the biosensor to remove the data card without shutting down, and then replace it with a new data card.

[0004] Although the above-mentioned existing technical solutions can be used as biosensors to detect corresponding items for chronic diabetic patients, with the research on nanocomposite materials, more and more nanocomposite materials are discovered and applied. Biosensor devices based on nanocomposite materials utilize the characteristics of nanocomposite materials to achieve the purpose of optimizing the detection effect of biosensor devices. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the shortcomings of the prior art, the present invention provides a biosensor device based on nanocomposite materials and a method of using the same, which utilizes the characteristics of graphene nanomaterials to form a composite carrier of biological detection bodies to optimize detection data and improve detection reliability and sensitivity. It also has a wide detection range and good practicality.

[0007] (II) Technical solution

[0008] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a biosensor device based on nanocomposite materials, comprising a sensor and a detection carrier, wherein the sensor is provided with an operating chamber, the detection carrier comprises a fixed column and a rotating sleeve, the fixed column is fixedly connected in the operating chamber, a rotation limit assembly is installed on the fixed column, the rotating sleeve is installed on the rotation limit assembly, and the rotating sleeve is arranged outside the fixed column, a plurality of branch pipes are fixedly connected to the outside of the rotating sleeve, a plurality of branch pipes are fixedly connected to a carrier box, a plurality of carrier boxes are provided with nanocomposite film, and a plurality of nanocomposite materials are arranged inside the carrier boxes. Biological detection bodies are fixed on the films, and the multiple biological detection bodies are different biological detection bodies. Electronic detection parts are installed in multiple carrier boxes, and the multiple electronic detection parts are electrically connected to the chip in the sensor. Multiple fan-shaped arc blocks are fixedly connected to the outside of the rotating sleeve, and a partition plate is arranged between each two adjacent fan-shaped arc blocks among the multiple fan-shaped arc blocks, and the partition plate is in sliding fit with the two adjacent fan-shaped arc blocks. A mask is installed on the sensor, and the top ends of the multiple partition plates are in sliding fit with the inside of the mask, and the bottom ends of the multiple partition plates are in sliding fit with the inner bottom wall support of the working chamber.

[0009] Preferably, the transfer limit assembly includes an elastic spring and a pushing sleeve, the elastic spring is fixedly connected in the working cavity, the top end of the elastic spring is connected to the rotating sleeve, the pushing sleeve is fixedly connected to the rotating sleeve, and an extension hole is provided on the mask, the top end of the pushing sleeve extends to the top of the mask through the extension hole, the top ends of the plurality of fan-shaped arc blocks are fixedly connected to the limiting protrusion, and a limiting groove matching the limiting protrusion is provided in the mask.

[0010] Preferably, the top end of the elastic spring is fixedly connected with a rotating link, and the top end of the rotating link is rotatably connected to the rotating sleeve.

[0011] Preferably, the top end of the pushing sleeve is fixedly connected to a pushing frame, and the top end of the pushing frame is provided with a finger pressure auxiliary operation groove.

[0012] Preferably, an inlet is provided on the mask.

[0013] Preferably, an opening and closing cover is slidably installed in the insertion port.

[0014] Preferably, a permanent magnet is embedded and fixedly connected to the opening and closing cover, the mask is made of a material that cannot be attracted by magnetism, and two iron blocks are arranged in the mask, and both of the two iron blocks match the permanent magnet.

[0015] A method for using a biosensor device based on a nanocomposite material comprises the following steps:

[0016] S1. When using, first turn on the sensor, which is provided with a switch key and a touch control display screen. Select the item to be tested and applied by touching the display screen, and adjust the specific parameters. Then insert the finger into the finger pressure auxiliary operation slot and press it, so that the push frame overcomes the elastic force of the elastic spring and moves down in the working chamber;

[0017] S2, the downward movement of the push frame will cause the fan-shaped arc block to move downward synchronously, and then the limit convex point will move downward and exit the limit groove, so that the relative limit effect between the rotating sleeve and the mask will be invalid, and the pressing state of the push frame will be maintained, and the push frame will be rotated to achieve synchronous drive adjustment of the rotating sleeve and multiple branch pipes, and then achieve synchronous relative adjustment of multiple carrier boxes, so as to achieve the position adjustment of the required supporting biological detection body, so that the corresponding biological detection body is rotated and adjusted to the position corresponding to the insertion port;

[0018] S3. Then, the sample to be tested is added into the carrier box located at the corresponding position of the inlet. The biological detection body reacts with the sample, and the electronic detection component transmits the corresponding detection information to the sensor to form a corresponding detection. Due to the catalytic effect of the use of the nanocomposite film on the biological detection body, the detection has high sensitivity and high stability.

[0019] (III) Beneficial effects

[0020] Compared with the prior art, the present invention provides a biosensor device based on nanocomposite materials and a method of using the same, which has the following beneficial effects:

[0021] 1. In the present invention, a composite carrier of a biological detection body is formed by preparing a nanocomposite film, so as to optimize the detection data and improve the detection reliability and sensitivity.

[0022] 2. In the present invention, a plurality of storage and detection spaces adapted to the nano-composite film and the biological detection body are formed by arranging a rotating sleeve, a plurality of branch pipes and a plurality of carrier boxes, and the detection range is relatively wide.

[0023] 3. In the present invention, through the design of the transfer limit assembly, the necessary limit structure is formed in cooperation with the rotating sleeve, which facilitates the adjustment of multiple carrier boxes and can also achieve auxiliary limit after the multiple carrier boxes are relatively adjusted. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0025] Figure 2 It is a schematic diagram of the three-dimensional structure of the rotating sleeve, branch pipe and carrier box of the present invention;

[0026] Figure 3 It is a schematic diagram of the three-dimensional structure of the sensor and the fixing column of the present invention;

[0027] Figure 4 It is a schematic diagram of the three-dimensional structure of the face mask and the iron block of the present invention cooperating with each other;

[0028] Figure 5 It is a schematic diagram of the three-dimensional structure of the rotating sleeve, the branch pipe and the elastic spring of the present invention;

[0029] Figure 6 It is a schematic diagram of the three-dimensional structure of the present invention as a whole when viewed from above;

[0030] Figure 7 It is a schematic diagram of the three-dimensional structure of the face mask of the present invention when viewed from above;

[0031] Figure 8 It is a bottom-up schematic diagram of the three-dimensional structure of the branch pipe, the fan-shaped arc block and the elastic spring of the present invention.

[0032] In the figure: 1. sensor; 2. working chamber; 3. fixed column; 4. rotating sleeve; 5. branch pipe; 6. carrier box; 7. electronic detection component; 8. fan-shaped arc block; 9. partition plate; 10. mask; 11. elastic spring; 12. push sleeve; 13. extension hole; 14. limiting convex point; 15. limiting groove; 16. rotating chain; 17. push frame; 18. finger pressure auxiliary operation groove; 19. insertion entrance; 20. opening and closing cover; 21. permanent magnet; 22. iron block. DETAILED DESCRIPTION

[0033] Example

[0034] See also Figure 1-Figure 8A biosensor device based on nanocomposite materials comprises a sensor 1 and a detection carrier, wherein the sensor 1 is provided with a working chamber 2, the detection carrier comprises a fixed column 3 and a rotating sleeve 4, the fixed column 3 is fixedly connected in the working chamber 2, a transfer limit assembly is installed on the fixed column 3, the rotating sleeve 4 is installed on the transfer limit assembly, the transfer limit assembly comprises an elastic spring 11 and a pushing sleeve 12, the elastic spring 11 is fixedly connected in the working chamber 2, the top end of the elastic spring 11 is connected to the rotating sleeve 4, the top end of the elastic spring 11 is fixedly connected with a rotating link 16, the top end of the rotating link 16 is rotatably connected to the rotating sleeve 4, the pushing sleeve 12 is fixedly connected to the rotating sleeve 4, and a protruding portion is provided on the mask 10 Hole 13, the top of the push sleeve 12 extends out to the top of the mask 10 through the extension hole 13, the tops of the multiple fan-shaped arc blocks 8 are fixedly connected to the limiting protrusions 14, and the mask 10 is provided with limiting grooves 15 matching the limiting protrusions 14. Through the design of the transfer limiting component, the necessary limiting structure is formed in cooperation with the rotating sleeve 4, which facilitates the adjustment of multiple carrier boxes 6 and can also realize the auxiliary limiting of multiple carrier boxes 6 after relative adjustment. The rotating sleeve 4 is sleeved outside the fixed column 3, and the rotating sleeve 4 is fixedly connected to the outside of the rotating sleeve 4. The multiple branch pipes 5 are fixedly connected to the carrier boxes 6. Through the configuration of the rotating sleeve 4, multiple branch pipes 5 and multiple carrier boxes 6, multiple storage and detection spaces adapted to nano-composite film and biological detection bodies are formed to detect The range is relatively wide, and nanocomposite films are arranged in multiple carrier boxes 6. A composite carrier of biological detection bodies is formed by the arrangement of nanocomposite films, so as to optimize the detection data and improve the detection reliability and sensitivity. Biological detection bodies are fixed on multiple nanocomposite films, and multiple biological detection bodies are different biological detection bodies. Electronic detection parts 7 are installed in multiple carrier boxes 6, and multiple electronic detection parts 7 are electrically connected to the chip in the sensor 1. Multiple fan-shaped arc blocks 8 are fixedly connected to the outside of the rotating sleeve 4, and a partition plate 9 is arranged between each adjacent two fan-shaped arc blocks 8 in the multiple fan-shaped arc blocks 8. The partition plate 9 is in sliding cooperation with its two adjacent fan-shaped arc blocks 8, and a mask is installed on the sensor 1. 10, the top ends of the plurality of partition plates 9 are slidably matched with the interior of the mask 10, the bottom ends of the plurality of partition plates 9 are slidably matched with the inner bottom wall support of the working chamber 2, the top end of the pushing sleeve 12 is fixedly connected with a pushing frame 17, the top end of the pushing frame 17 is provided with a finger pressure auxiliary operation groove 18, which is convenient for the insertion operation of the finger, the mask 10 is provided with an insertion inlet 19, which can allow the insertion of the sample, and an opening and closing cover 20 is slidably installed in the insertion inlet 19, and a permanent magnet 21 is embedded and fixedly connected to the opening and closing cover 20, the mask 10 is made of a material that cannot be attracted by magnetism, and two iron blocks 22 are arranged in the mask 10, and the two iron blocks 22 are matched with the permanent magnet 21, which is convenient for the opening and closing cover 20 to limit the position after opening and closing. Specific embodiment 1

[0036] Glucose Oxidase Sensor

[0037] A glucose oxidase sensor 1 is formed based on a graphene nanocomposite material, a new enzyme immobilization matrix is ​​constructed, and the performance of the biological sensor is enhanced. The surface of the graphene nanocomposite material thus produced has an obvious electrocatalytic effect on hydrogen peroxide and oxygen. In addition, when glucose oxidase is immobilized in the composite film of the graphene nanocomposite material, the electrode thus produced shows a good linear response to glucose. The graphene nanocomposite material can promote the electrocatalytic effect on hydrogen peroxide. The modified electrode is successfully assembled into a practical glucose biosensor with high sensitivity and high stability. Specific embodiment 2

[0039] Immunobiosensors

[0040] A new immunosensing platform based on a nanocomposite material of graphene sheets and thionine (TH) is used to prepare a label-free electrochemical immunosensor. The surface area of ​​graphene can increase the amount of adsorption to a certain extent, and its good conductivity can also enhance the electrical activity of thionine. AFP is used as a model, and the change in peak current when the antigen and antibody act after the modification of thionine is used for detection. This immunosensing method is simple and easy, and has the opportunity to become an application for ultra-sensitive detection of different tumor markers.

[0041] By constructing a new label-free electrochemical immunosensor for detecting carcinoembryonic antigen, the surface of the glassy carbon electrode was modified with a graphene-toluidine blue complex. The test results showed that the detection limit of this method was 0.1 μg·L -1 The sensor has good reproducibility, selectivity and stability and is used for the determination of human serum samples. Heme-modified graphene nanosheets (HGN) are used as electrochemical immunosensor 1 to determine L-tyrosine. The detection limit of the immunosensor is 7.5x10 - 8 mol·L -1 , the linear range is 5x10 -7 -2x10 -5 mol·L -1 .

[0042] In summary, the method for using the biosensor device based on nanocomposite materials is as follows: when using, first turn on the sensor 1, which is provided with an on / off key and a touch control display screen, select the item to be detected and applied by touching the display screen, and adjust the specific parameters, then insert the finger into the finger pressure auxiliary operation groove 18, and press it, so that the push frame 17 overcomes the elastic force of the elastic spring 11 and forms a relative position downward movement in the working chamber 2, and the downward movement of the push frame 17 will cause the fan-shaped arc block 8 to move downward synchronously, and then the limiting protrusion 14 will move downward and exit the limiting groove 15, so that the relative limiting effect between the rotating sleeve 4 and the mask 10 is invalid, and the pressing state of the push frame 17 is maintained. , and rotate the push frame 17 to achieve synchronous drive adjustment of the rotating sleeve 4 and multiple branch pipes 5, and then achieve synchronous relative adjustment of multiple carrier boxes 6, so as to achieve position adjustment of the required supporting biological detection body, so that the corresponding biological detection body is rotated and adjusted to the position corresponding to the insertion port 19, and then the sample to be detected is added to the carrier box 6 located at the corresponding position of the insertion port 19, the biological detection body reacts with the sample, and the electronic detection component 7 transmits the corresponding detection information to the sensor 1, forming a corresponding detection. Due to the catalytic effect of the use of the nano-composite film on the biological detection body, the detection has high sensitivity and high stability.

Claims

1. A biosensor device based on a nanocomposite material, comprising a sensor (1), characterized in that: The sensor (1) further comprises a detection carrier, wherein an operating chamber (2) is arranged on the sensor (1), and the detection carrier comprises a fixed column (3) and a rotating sleeve (4), wherein the fixed column (3) is fixedly connected in the operating chamber (2), a rotation limit assembly is installed on the fixed column (3), the rotating sleeve (4) is installed on the rotation limit assembly, and the rotating sleeve (4) is sleeved outside the fixed column (3), a plurality of branch pipes (5) are fixedly connected to the outside of the rotating sleeve (4), a plurality of branch pipes (5) are fixedly connected to a carrier box (6), a plurality of carrier boxes (6) are arranged in each of the plurality of nanocomposite film, a plurality of nanocomposite film are fixedly loaded with a biological detection body, and the plurality of biological detection bodies are different A biological detection body, wherein electronic detection components (7) are installed in multiple carrier boxes (6), and multiple electronic detection components (7) are electrically connected to the chip in the sensor (1). Multiple fan-shaped arc blocks (8) are fixedly connected to the outside of the rotating sleeve (4), and a partition plate (9) is arranged between each two adjacent fan-shaped arc blocks (8) in the multiple fan-shaped arc blocks (8), and the partition plate (9) and its two adjacent fan-shaped arc blocks (8) are in sliding fit. A mask (10) is installed on the sensor (1), and the top ends of the multiple partition plates (9) are in sliding fit with the inside of the mask (10), and the bottom ends of the multiple partition plates (9) are in sliding fit with the inner bottom wall support of the working chamber (2).

2. A biosensor device based on nanocomposite materials according to claim 1, characterized in that: The transfer limit assembly comprises an elastic spring (11) and a push sleeve (12), wherein the elastic spring (11) is fixedly connected in the working chamber (2), the top end of the elastic spring (11) is connected to the rotating sleeve (4), the push sleeve (12) is fixedly connected to the rotating sleeve (4), and an extension hole (13) is provided on the mask (10), the top end of the push sleeve (12) extends to the top of the mask (10) through the extension hole (13), the top ends of the plurality of fan-shaped arc blocks (8) are fixedly connected to the limit protrusion (14), and the mask (10) is provided with a limit groove (15) matching the limit protrusion (14).

3. A biosensor device based on nanocomposite materials according to claim 2, characterized in that: The top end of the elastic spring (11) is fixedly connected to a rotating link (16), and the top end of the rotating link (16) is rotatably connected to the rotating sleeve (4).

4. The biosensor device based on nanocomposite material according to claim 3, characterized in that: The top end of the push sleeve (12) is fixedly connected to a push frame (17), and the top end of the push frame (17) is provided with a finger pressure auxiliary operation groove (18).

5. The biosensor device based on nanocomposite material according to claim 4, characterized in that: The mask (10) is provided with an insertion opening (19).

6. The biosensor device based on nanocomposite material according to claim 5, characterized in that: An opening and closing cover (20) is slidably mounted in the insertion opening (19).

7. The biosensor device based on nanocomposite material according to claim 6, characterized in that: A permanent magnet (21) is embedded and fixedly connected to the opening and closing cover (20); the mask (10) is made of a material that cannot be attracted by magnetism; and two iron blocks (22) are arranged inside the mask (10); the two iron blocks (22) are matched with the permanent magnet (21).

8. A method for using a biosensor device based on a nanocomposite material, characterized in that: A biosensor device based on a nanocomposite material according to any one of claims 1 to 7 is used, comprising the following steps: S1. When in use, first turn on the sensor (1). The sensor (1) is provided with an on / off key and a touch control display screen. The item to be detected and applied is selected by touching the display screen, and specific parameters are adjusted. Then, a finger is inserted into the finger pressure auxiliary operation slot (18) and pressed, so that the push frame (17) overcomes the elastic force of the elastic spring (11) and moves downward in a relative position in the working chamber (2); S2, the downward movement of the push frame (17) causes the fan-shaped arc block (8) to move downward synchronously, and then the limiting protrusion (14) moves downward and exits the limiting groove (15), so that the relative limiting effect between the rotating sleeve (4) and the mask (10) becomes invalid, the pushing frame (17) is kept in a pressed state, and the pushing frame (17) is rotated to achieve synchronous drive adjustment of the rotating sleeve (4) and the plurality of branch pipes (5), and then the synchronous relative adjustment of the plurality of carrier boxes (6) is achieved, so as to achieve position adjustment of the biological detection body required for matching use, so that the corresponding biological detection body is rotated and adjusted to the position corresponding to the insertion port (19); S3. Then, the sample to be tested is added to the carrier box (6) located at the corresponding position of the insertion port (19). The biological test body reacts with the sample, and the electronic detection element (7) transmits the corresponding detection information to the sensor (1), thereby forming a corresponding detection. Due to the catalytic effect of the nanocomposite film on the biological test body, the detection has high sensitivity and high stability.

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