Breeding method and application of rice with dynamically changed glume colors

Abstract

The invention discloses a glume color dynamic change rice breeding method and application, and belongs to the technical field of rice breeding. The breeding method comprises the following steps: taking red rice green glume indica rice as a female parent and white rice indica rice as a male parent, and carrying out sexual hybridization to obtain an F1 generation; planting the F1-generation seeds, performing anther culture on young ears in a differentiation period, and treating calluses by using fast neutrons; performing colchicine solution treatment on the treated calluses in a culture medium to obtain suspension cells; the obtained suspension cells are differentiated into seedlings, single plants with green glumes before grain filling, red glumes after grain filling, short flag leaves and high ear height after heading are selected for the H2 generation, continuous breeding is conducted till an H5 generation strain is obtained, and rice with the dynamically-changed glume colors is obtained. According to the method, the problem that the patterns of the rice field picture are unchanged after being planted is solved, the rice field picture can present different patterns along with the change of seasons and growth cycles, and the artistic expressive force and the ornamental value of the rice field picture are improved.

Description

Technical Field

[0001] The invention relates to the field of rice breeding and rural art technology, and in particular to a rice breeding method with dynamic change in husk color and application thereof. Background Art

[0002] As people's living standards improve, the aesthetic demand for agricultural landscapes is also increasing. As a new form of agricultural landscape, rice field paintings have become widely popular in the rice-growing area. Traditional rice field paintings form patterns or words by planting rice of different colors, and the lines used to draw rice field paintings are rice varieties of different colors.

[0003] At present, researchers have cultivated a variety of non-green rice varieties such as purple, red, yellow, and white. These colored rices enrich the line colors of rice field paintings and increase the richness of rice field paintings. However, due to the limitation of rice varieties, the current rice field paintings still have certain limitations in the richness of the pictures. For example, on the one hand, as a photosynthetic plant, rice is limited to not being too rich in color, which in turn reduces the richness and polychromaticity of the colors of rice field paintings; on the other hand, the planting of rice field paintings is time-consuming and laborious, and the color of a specific variety of rice is fixed. After the seedlings are planted, the content of the rice field painting will no longer change during the entire growing season. This results in the pattern of the rice field painting being unchanged from the completion of the planting of the rice field painting to the yellow maturity of the rice, which is an unfavorable factor that restricts the attraction of tourists to visit and consume many times in rural tourism. Summary of the invention

[0004] In order to solve the above technical problems, the purpose of the present invention is to provide a rice breeding method and application with dynamic change of husk color, so as to solve the problems that the existing rice field paintings are limited by rice varieties, there are certain limitations on the richness of the pictures, and the pattern of the rice field paintings remains unchanged from the completion of rice planting to the yellow maturity of rice.

[0005] The technical solution of the present invention to solve the above technical problems is as follows:

[0006] A rice breeding method with dynamic change in husk color comprises the following steps:

[0007] (1) using red rice with green hulls as the female parent and white rice as the male parent, sexually hybridizing to obtain the F1 generation;

[0008] (2) planting the F1 seeds obtained in step (1), taking the young spikelets in the differentiation stage for anther culture, and treating the callus tissue with fast neutrons;

[0009] (3) treating the callus tissue treated in step (2) with a colchicine solution in a culture medium to obtain suspended cells;

[0010] (4) Differentiating the suspension cells obtained in step (3) into seedlings, selecting H2 generation plants with green husks before grain filling, red husks after grain filling, short flag leaves and high panicle layer after heading to construct anther culture lines, and continuously selecting and breeding to H5 generation lines to obtain rice with dynamically changing husk color.

[0011] The beneficial effects of the present invention are as follows: the present invention provides a method for breeding rice varieties with dynamically changing husk colors and its application in improving the image richness of "rice field paintings". While increasing the diversity of rice varieties in rice field paintings, it innovatively proposes to change the pattern content of rice field paintings that remains unchanged after planting by dynamically changing the color of rice ears. This method can make the pattern in the rice field painting change as the growth period progresses, thereby increasing the viewing value.

[0012] Furthermore, the red rice with green hulls in step (1) is Guanghong No. 3.

[0013] Furthermore, the indica white rice in step (1) is Yuehe Silk Rice.

[0014] Furthermore, in step (2), the anthers are cultured until the callus tissue is in the logarithmic growth phase.

[0015] Furthermore, the neutron flux in the fast neutron treatment in step (2) is 0.8×10 11 -1.2×10 11 n / cm 2 .

[0016] The beneficial effect of adopting the above further technical solution is that the present invention increases the mutation rate of the offspring and promotes the progress of mutation breeding by treating callus tissue with fast neutrons.

[0017] Furthermore, the conditions for the colchicine solution treatment in step (3) are as follows: after the callus tissue is proliferated for one generation in the suspension culture medium, the suspension cells in the logarithmic growth phase are treated with a colchicine solution having a mass concentration of 0.8-1.2% for 20-30 hours.

[0018] Furthermore, the breeding target in step (4) is individuals whose husk color is green before grain filling, gradually turns red after grain filling, have short flag leaves, and have a high ear layer after heading.

[0019] Furthermore, in step (4), the target individual plants selected for breeding have high ear layers and short flag leaves after heading.

[0020] The application of the rice with dynamic husk color change bred by the above-mentioned rice breeding method with dynamic husk color change in rice field painting.

[0021] Furthermore, the image richness of the rice field painting is improved by dynamically changing the husk color of the rice.

[0022] The present invention has the following beneficial effects:

[0023] The present invention hybridizes red rice green glume indica rice with white rice indica rice, performs anther culture and fast neutron mutagenesis on the hybrid offspring, and continuously breeds the rice variety XKH-1 whose glume changes to red gradient color after filling, thereby enriching the rice varieties suitable for rice field paintings, solving the problem that the patterns of rice field paintings remain unchanged after planting, enabling the rice field paintings to present different patterns with the changes of seasons and growth cycles, and greatly improving the artistic expression and ornamental value of the rice field paintings. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The hull gradient color rice variety XKH-1 bred in Example 1 of the present invention, wherein a is a plant before filling, b is a plant after filling, c is a grain of the plant before filling, and d is a grain of the plant after filling;

[0025] Figure 2 This is a schematic diagram of the state of a rice field painting "smiley face" obtained from the hull gradient color rice variety bred in Example 1 of the present invention in the early stage of planting;

[0026] Figure 3 This is a schematic diagram of the state of a rice field painting "smiley face" in the late grain filling stage of the rice variety with gradient hull color bred in Example 1 of the present invention. DETAILED DESCRIPTION

[0027] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples are only used to explain the present invention and are not used to limit the scope of the present invention. If specific conditions are not specified in the embodiments, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0028] Embodiment 1:

[0029] A rice breeding method with dynamic change in husk color comprises the following steps:

[0030] (1) Using a red rice variety with green hulls (Yuehe Si Miao, purchased from Sichuan Taiwo Seed Co., Ltd.) as the female parent and a white rice variety with green hulls (Guanghong No. 3, introduced from the Rice Institute of Guangdong Academy of Agricultural Sciences) as the male parent, sexual hybridization was performed to obtain the F1 generation;

[0031] (2) After planting the F1 seeds obtained in step (1), young spikelets in the ear differentiation stage were taken for anther culture. When the callus tissue of anther development reached the logarithmic growth stage, 1×10 11 n / cm 2Treating callus with fast neutrons increases the mutation rate of offspring; treating callus after fast neutron treatment with 1% colchicine solution in suspension medium for 24 hours to induce haploid cell doubling; and transferring the obtained doubled suspension cells to solid and then differentiating into seedlings.

[0032] (3) selecting individual plants that meet the target traits (green husk before grain filling, red husk after grain filling, and relatively short flag leaf) from the anther seedlings obtained in step (2) in the H2 generation to construct anther culture strain, repeatedly selecting individual plants and establishing strains in subsequent generations, and continuously selecting strains to the H5 generation to obtain strains with stable agronomic traits; performing yield comparison, selecting strains with higher yields, and finally obtaining an indica rice germplasm resource material with green husk before grain filling, red husk after grain filling, higher panicle layer after heading, and shorter flag leaf, such as Figure 1 As shown, it was named XKH-1.

[0033] An application of a rice variety with dynamically changing husk color to improving the image richness of a "rice field painting" includes the following steps:

[0034] The husk gradient color rice XKH-1 obtained in step (3) is used as a "hidden line" when the rice seedlings are planted in the rice field. This embodiment takes a simple rice field painting "smiley face" as an example (such as Figure 2 and Figure 3 ), before the rice field painting was filled with grain, the pattern of the rice field painting was only a "smiley face", and the husk gradient color rice XKH-1 was planted at the "dimple", but because of the strong filling, the color displayed by XKH-1 was consistent with the conventional background varieties (all green), so the overall "smiley face" had no dimples.

[0035] In the painting of the rice field in the late stage of rice filling, the glume of rice XKH-1 changes color due to purple-red color when the grains are filling. Because the rice ears of this variety have a high layer, the color of the rice ears is not covered by the leaves; therefore, red dimples appear in the "smiley face" dimples.

[0036] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A rice breeding method with dynamic change in husk color, characterized in that: The following steps are involved: (1) using red rice with green hulls as the female parent and white rice as the male parent, sexually hybridizing to obtain the F1 generation; (2) planting the F1 seeds obtained in step (1), taking the young spikelets in the differentiation stage for anther culture, and treating the callus tissue with fast neutrons; (3) treating the callus tissue treated in step (2) with a colchicine solution in a culture medium to obtain suspended cells; (4) Differentiating the suspension cells obtained in step (3) into seedlings, selecting H2 generation plants with green husks before grain filling, red husks after grain filling, short flag leaves and high panicle layer after heading to construct anther culture lines, and continuously selecting and breeding to H5 generation lines to obtain rice with dynamically changing husk color.

2. The rice breeding method with dynamic change in husk color according to claim 1, characterized in that: The red rice with green hulls in step (1) is Guanghong No.

3.

3. The rice breeding method with dynamic change of husk color according to claim 1, characterized in that: The indica white rice in step (1) is Yuehe Silk Rice.

4. The rice breeding method with dynamic change in husk color according to claim 1, characterized in that: In the step (2), the anthers are cultured until the callus reaches the logarithmic growth phase.

5. The rice breeding method with dynamic change of husk color according to claim 1, characterized in that: The neutron flux of the fast neutron treatment in step (2) is 0.8×10 11 -1.2×10 11 n / cm 2 .

6. The rice breeding method with dynamic change in husk color according to claim 1, characterized in that: The conditions for the colchicine solution treatment in step (3) are as follows: after the callus tissue is proliferated for one generation in the suspension culture medium, the suspension cells in the logarithmic growth phase are treated with a colchicine solution having a mass concentration of 0.8-1.2% for 20-30 hours.

7. The rice breeding method with dynamic change in husk color according to claim 1, characterized in that: The breeding target in step (4) is individuals whose husk color is green before grain filling, gradually turns red after grain filling, have short flag leaves, and have a high ear layer after heading.

8. Use of the rice with dynamically changing husk color bred by the method for breeding rice with dynamically changing husk color according to any one of claims 1 to 7 in rice field painting.

9. The use according to claim 8, characterized in that: The image richness of rice field paintings is improved by dynamically changing rice husk color.

Images