Preparation method of hydrogel nucleic acid separation device

Abstract

The invention belongs to the technical field of engineering, and provides a preparation method of a nucleic acid separation device. According to the preparation method disclosed by the invention, the hydrogel nucleic acid adsorption device is prepared through the photo-initiated free radical polymerization reaction, so that the tedious limitation that the traditional polymerization reaction needs to be carried out under an anaerobic condition is avoided. The preparation method has the advantages of cheap and easily available raw materials, simple and rapid preparation process, no dependence on professional and precise instruments, high-efficiency loading of silicon dioxide particles, effective combination of the plasticity of the hydrogel, the high specific surface area of the silicon dioxide particles and the strong binding capacity of nucleic acid, and high stability. The shape, the size and the number of the handheld nucleic acid extraction device can be changed at any time according to application requirements, and the handheld nucleic acid extraction device has high specific surface area and excellent nucleic acid extraction performance. Compared with a traditional process, the preparation process is simpler, the application range is wider, the application requirement of a low-resource environment is met to a great extent, and the method has a wide application prospect in the technical field of outdoor and clinical nucleic acid extraction.

Description

Technical Field

[0001] The invention belongs to the field of engineering technology and relates to a method for preparing a hydrogel nucleic acid separation device. Background Art

[0002] Nucleic acid is a kind of high molecular compound widely present in all living organisms, including two major categories: DNA and RNA. It plays a decisive role in a series of major life phenomena such as growth, inheritance, and mutation. The methods for extracting nucleic acid include liquid phase separation and solid phase method (magnetic separation and non-magnetic separation). Among them, the solid phase separation method generally uses materials with nucleic acid adsorption properties, such as magnetic beads, silica, cellulose, silica gel, or synthetic polymer materials specially designed for nucleic acid extraction. The commonly used methods at present are mainly magnetic bead method (magnetic separation) and centrifugal column method (non-magnetic separation) to extract nucleic acid. The magnetic bead method used in application number CN201710113885.X "A method for extracting nucleic acids by magnetic beads" separates nucleic acids. Silica-coated magnetic particles (positively charged) are used to adsorb nucleic acids (negatively charged) and then repeatedly blown and washed in a lysate. After adding a magnetic field, the magnetic beads are adsorbed to the tube wall, the liquid is removed, and the adsorption-washing-desorption steps of the nucleic acid are repeated; application number 202210295109.7 discloses a nucleic acid extraction centrifuge column tube, a nucleic acid extraction device and a nucleic acid extraction method, in which nucleic acids are extracted using a centrifuge column, wherein the centrifuge column is mostly made of silicon-based materials or polymer materials, and nucleic acids are adsorbed by electrostatic action, hydrogen bonding and intermolecular forces, and the nucleic acid lysate, washing solution and elution solution pass through the adsorption material to achieve the separation of nucleic acids by relying on centrifugation technology. The above two methods are currently the more commonly used methods for separating nucleic acids, but the magnetic bead method of magnetic separation relies on the magnetic field, and the magnetic particles aggregate and settle; the non-magnetic centrifugal column method requires multiple auxiliary operations, relies on centrifugation technology, takes a long time to separate, has low processing throughput, and introduces PCR inhibitory components during the process.

[0003] To solve the above problems, the applicant has previously disclosed a non-magnetic nucleic acid solid phase separation technology based on 3D printing functional devices, which is efficient and does not rely on centrifugation technology and external magnetic fields to extract biological sample DNA with high throughput and high quality. However, the preparation of 3D printed devices relies on high-precision and expensive 3D printers, which has high professional requirements for preparation personnel, and the development cycle of inks matching 3D printing is long, which is not friendly to low-resource areas. Therefore, it is particularly important for low-resource areas to develop a simple preparation method for nucleic acid extraction devices that do not require instruments. Summary of the invention

[0004] The present invention provides a novel method for preparing a nucleic acid separation device. The preparation method is simple and fast, and the shape of the device can be flexibly adjusted as needed to solve the problem that traditional nucleic acid separation relies on centrifugation and has low throughput. The method is a method for preparing a nucleic acid separation device when the laboratory does not have the materials to construct an instrument.

[0005] The technical solution of the present invention:

[0006] A method for preparing a hydrogel nucleic acid separation device, comprising the following steps:

[0007] (1) adding a crosslinking agent and a photoinitiator to a hydrogel monomer to prepare a prepolymer solution;

[0008] (2) mixing the prepolymer solution and adding it to the mold, inserting the handle, placing it under a UV lamp for irradiation and curing, and performing a free radical polymerization reaction under the light condition;

[0009] (3) Hold the handle to remove the device from the mold, wash and dry the device, and then store it.

[0010] The hydrogel monomer is a commonly used hydrogel monomer such as acrylic acid and acrylate; the crosslinking agent is a commonly used crosslinking agent such as N'N methylene bisacrylamide and polyethylene glycol diacrylate, and the crosslinking agent is 2-50wt% of the hydrogel monomer; the initiator adopts a photoinitiator (2,4,6-trimethylbenzoyl) diphenylphosphine oxide, etc., and the added amount is 0.1-0.5wt% of the hydrogel monomer.

[0011] Preferably, silica particles can be added to the above prepolymer solution; the silica particles can be a single component of silica or other substances with silica as the main component, such as quartz stone, glass, and their size is not limited. Preferably, the particle size of the silica particles is a mixture of quartz sand particles of 50 mesh to 180 mesh, and the amount of silica particles added is 20-50wt% of the total solid-liquid mixture, and the particle structure can achieve a rough frosted material on the surface of the hydrogel device.

[0012] The photo-initiated free radical polymerization reaction is carried out at room temperature, the wavelength of light is 280-400nm, the irradiation time is 3-10min, the purpose of inverting the mold is to make the bottom of the device cross-linked evenly, and the irradiation time is 3-40min; after demolding and taking out, the device is washed, and the washing liquid uses one or more of water and ethanol, and the number of washing times is not less than one, to remove excess monomers and impurities in the device and reduce the risk of contamination in subsequent use. The washed device is dried at a drying temperature of 0-80° and a drying time of 3min-24h; the device is stored at room temperature and away from light.

[0013] The mold is a container with a cavity that matches the needs of DNA extraction in the later stage, preferably a centrifuge tube, an eight-well tube strip, a 96-well plate, and a deep-well plate. If a handheld device is prepared, the handle part is rod-shaped, and the shape and material are not limited.

[0014] Beneficial effects of the present invention:

[0015] (1) The present invention prepares hydrogel nucleic acid adsorption devices through photoinitiated free radical polymerization, avoiding the tedious and complicated polymerization under anaerobic conditions in traditional processes. Compared with centrifugal column-type and magnetic particle nucleic acid extraction carriers and devices, the raw materials are simple, cheaper and easier to obtain, the preparation process is simple and fast, and does not rely on other professional materials to build equipment, which is suitable for low-resource areas and outdoor application needs.

[0016] (2) The device uses hydrogel as the substrate. The surface groups of the hydrogel polymer material are rich, which can increase the electrostatic effect, hydrogen bonding effect and intermolecular force of the adsorbed nucleic acids to achieve efficient separation of nucleic acids. By loading silica particles, the plasticity of the hydrogel material and the high nucleic acid adsorption of silica are combined to further improve the nucleic acid adsorption performance.

[0017] (3) This preparation method is simple and easy, does not rely on machines, and can efficiently load functional particles. It solves the defect of traditional hydrogel shaping processes such as 3D printers that solid particles added to the printing ink can easily damage the machine. At the same time, the surface roughness and specific surface area are increased by doping particles, which further increases the nucleic acid binding sites and improves the nucleic acid extraction performance of the device.

[0018] (4) This preparation method can realize the preparation of nucleic acid extraction devices of different quantities, sizes, and shapes by changing the mold, matching different containers for use, and having a wider range of application scenarios.

[0019] (5) The device prepared in this way has a simple extraction process for nucleic acid and does not rely on instruments. Compared with the traditional centrifugal column and magnetic separation nucleic acid extraction methods, it does not rely on professional equipment and does not have the risk of particle agglomeration and sedimentation. It can meet the application needs of nucleic acid extraction in low-resource environments, outdoor and clinical settings to a great extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a hydrogel nucleic acid adsorption device; among them, (a) a single nucleic acid extraction device; (b) a nucleic acid extraction device adapted for eight-row tubes; (c) a top view of a nucleic acid extraction device adapted for a 1.5mL centrifuge tube; (d) a side view of a nucleic acid extraction device adapted for a 1.5mL centrifuge tube.

[0021] Figure 2 These are the electrophoresis results of the device with DMAM as the cross-linking agent. 1-3: three sets of parallel results of the device without adding quartz sand, 4-6: three sets of parallel results of the device with adding quartz sand; M: DNA Maker; NC: negative control with water as template.

[0022] Figure 3It is the nucleic acid adsorption performance of the device with PEGDA as the cross-linker; among them, (a) the nucleic acid extraction concentration of the device with added silica (PAA-SiO2) and without added silica (PAA); (b) A260 / A280 and A260 / A230 of the device with added silica (PAA-SiO2) and without added silica (PAA).

[0023] Figure 4 It is the nucleic acid adsorption performance of the nucleic acid extraction device matched with the eight-tube strip; wherein, (a) the nucleic acid extraction concentration of the eight devices; (b) the A260 / A280 and A260 / A230 of the nucleic acid extracted by the eight devices.

[0024] Figure 5 The nucleic acid adsorption performance of the centrifuge tube type nucleic acid extraction device; wherein, (a) the nucleic acid extraction concentration at different silica addition amounts; and (b) the A260 / A280 and A260 / A230 at different silica addition amounts.

[0025] Figure 6 The nucleic acid adsorption performance of devices with different silica addition amounts; (a) nucleic acid extraction concentration of devices with different silica addition amounts; (b) A260 / A280 and A260 / A230 of devices with different silica addition amounts. DETAILED DESCRIPTION

[0026] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments;

[0027] The following is a further description of the present invention in conjunction with the technical solution. It should be noted that the following description is exemplary and is intended to provide further description of the present invention. Unless otherwise specified, all scientific and technical terms used in the present invention have the same meaning as those commonly understood by those skilled in the art to which the present invention belongs.

[0028] Unless otherwise specified, the materials, practices and experimental equipment involved in the implementation cases of the present invention are all in line with commercially available products in the relevant chemical and biotechnology fields.

[0029] Example 1

[0030] (1) 2 ml of acrylic acid monomer and 0.05 g of crosslinking agent N'N-methylenebisacrylamide (DMAM) were added, and then 0.002 g of photoinitiator and deionized water were added to prepare a prepolymer solution;

[0031] (2) The prepolymer solution in step (1) is divided into two groups, one group is added with 1 g of silica particles of 50-80 mesh quartz sand and 120-180 mesh quartz sand, and stirred evenly, and the other group is not added with quartz sand;

[0032] (3) After mixing, add (2) to a single 0.2 mL centrifuge tube, with 80 μL of the solid solution mixture in each well, and mix well;

[0033] (4) inserting a toothpick into the centrifuge tube as a handle in step (3), placing it under a 400 nm ultraviolet lamp for irradiation and curing for 5 minutes, and performing a free radical polymerization reaction under the light condition;

[0034] (5) Take out the centrifuge tube in (4), invert and irradiate the bottom for 3 min, and irradiate the sides for 3 min;

[0035] (6) Use the handle to demold the device from the centrifuge tube and wash it three times with anhydrous ethanol;

[0036] (7) Dry at 50℃ for 10 min and store away from light for later use (such as Figure 1 a).

[0037] The obtained polyacrylic acid doped silica nucleic acid extraction device is used to extract nucleic acid from biological samples, and the steps are divided into four steps: lysis-adsorption-washing-elution.

[0038] (1) Prepare CTAB nucleic acid lysis buffer and mix by centrifuge.

[0039] (2) Place the biological sample to be tested in a 1.5 mL centrifuge tube, add 400 μL of lysis buffer, and add 20 μL of proteinase K and shake well.

[0040] (3) Place at 60°C for 60 min until the biological sample tissue is completely dissolved and the solution becomes clear;

[0041] (4) Take 100 μL of the lysed solution and add 60 μL of isopropanol into a 0.2 mL centrifuge tube. Insert the device into the centrifuge tube and gently shake it up and down for 1 min before taking it out.

[0042] (5) Take a new 0.2 mL centrifuge tube and add 150 μL of washing solution. Add the device with adsorbed nucleic acid into the tube and gently shake it up and down for 30 seconds. Then take it out and place it at room temperature to dry.

[0043] (6) Take a new 0.2 mL centrifuge tube, add 80 μL of elution buffer and the dried device, and shake it up and down for 1 min to elute the nucleic acid from the device.

[0044] The obtained nucleic acid samples were used for PCR amplification and then electrophoresis test. The results obtained proved (such as Figure 2), the present invention prepares hydrogel nucleic acid adsorption devices through photoinitiated free radical polymerization, avoiding the cumbersome and complicated polymerization under anaerobic conditions in traditional processes. The raw materials are simple, cheap and easily available, the preparation process is simple and fast, and it does not rely on other professional materials to build equipment. The prepared device can selectively separate nucleic acids from mixed biological samples, which is suitable for low-resource areas and outdoor applications.

[0045] This embodiment also shows that the preparation method can be independent of the machine and efficiently load functional particles, which solves the defect of traditional hydrogel shaping processes such as 3D printers that solid particles are easily damaged by the printing ink. At the same time, the surface roughness and specific surface area are increased by doping particles, which further increases the nucleic acid binding sites and improves the nucleic acid extraction performance of the device ( Figure 3 ).

[0046] Example 2

[0047] (1) 2 mL of acrylic acid monomer and 1 mL of cross-linking agent polyethylene glycol diacrylate were added, and then 0.004 g of photoinitiator TPO was added to prepare a prepolymer solution;

[0048] (2) Add 1 g of 50-80 mesh quartz sand and 1 g of 120-180 mesh quartz sand to the prepolymer solution in step (1) and stir evenly;

[0049] (3) After mixing, add (2) to the eight-well tube strip, with 80 μL of the solid solution mixture in each well, and mix well;

[0050] (4) inserting the eight-row tube in step (3) into a comb-shaped handle, placing it under a 300 nm ultraviolet lamp for irradiation and curing for 5 minutes, and performing a free radical polymerization reaction under the light condition;

[0051] (5) Take out the eight-row tube in (4), invert and irradiate the bottom for 10 min, and irradiate the side for 10 min;

[0052] (6) Hold the handle to demould the device from the eight-row tube, and wash it three times with deionized water and anhydrous ethanol; (7) Dry it at 25°C for 24 hours and store it away from light for later use (such as Figure 1 b).

[0053] The obtained nucleic acid extraction device is used to extract nucleic acid from a biological sample, and a commercially available nucleic acid lysis solution is used to lyse the biological sample.

[0054] (1) Place the biological sample to be tested in a 1.5 mL centrifuge tube, add 400 μL of lysis buffer, and add 20 μL of proteinase K and shake well.

[0055] (2) Place at 56°C for 40 min until the biological sample tissue is completely dissolved and the solution becomes clear;

[0056] (3) Take 100 μL of the lysed solution and add 60 μL of isopropanol into an eight-tube strip;

[0057] (4) Insert the device into the eight-row tube and gently shake it up and down for 1 min before taking it out;

[0058] (5) Take a new 0.2 mL eight-tube strip and add 150 μL of 75 wt% ethanol. Add the device with adsorbed nucleic acid into the strip and gently shake it up and down for 30 seconds. Then take it out and place it at room temperature to dry.

[0059] (6) Take a new eight-tube strip, add 80 μL of TE elution solution and the dried device, and shake it up and down for 1 min to elute the nucleic acid from the device.

[0060] The obtained nucleic acid samples were used for quantification by UV spectrophotometer, and the concentrations of extracted nucleic acids were 45.77ng / μL, 37.96ng / μL, 31.78ng / μL, 26.78ng / μL, 25.01ng / μL, 30.59ng / μL, 37.95ng / μL, and 33.41ng / μL, respectively. The results show that the preparation method can realize the preparation of nucleic acid extraction devices of different quantities, sizes, and shapes by changing the mold, matching different containers for use, and the applicable scenarios are more extensive and flexible; the device prepared by using 50wt% polyethylene glycol diacrylate as a cross-linking agent and 40wt% silica addition has a high yield of nucleic acid extraction.

[0061] Example 3

[0062] (1) Take 2 mL of acrylic acid monomer and 0.1 g of crosslinking agent N'N-methylenebisacrylamide (PAA-SiO2-1), and another group with 0.2 g of crosslinking agent N'N-methylenebisacrylamide (PAA-SiO2-2), add 0.004 g of photoinitiator, and mix with deionized water to prepare a prepolymer solution;

[0063] (2) Add 1 g of 50-80 mesh quartz sand and 1 g of 120-180 mesh quartz sand to the prepolymer solution in step (1) and stir evenly;

[0064] (3) After mixing, add (2) to a 1.5 mL centrifuge tube, add 200 μL of the above prepolymer solution to each well, and insert a new 1.5 mL centrifuge tube so that the solid-liquid mixture is "squeezed" onto the wall;

[0065] (4) The centrifuge tube in step (3) is placed under a 300 nm ultraviolet lamp for irradiation and curing for 5 minutes to perform a free radical polymerization reaction under the illumination condition;

[0066] (5) Take out the two centrifuge tubes in (4), invert and irradiate the bottom for 3 min, and irradiate the side for 3 min;

[0067] (6) Remove the upper centrifuge tube and wash the inner wall of the centrifuge tube three times with deionized water;

[0068] (7) Dry at 80℃ for 3 min and store away from light for later use (such as Figure 1 c, d).

[0069] The obtained nucleic acid extraction device is used to extract nucleic acid from a biological sample, and a commercially available nucleic acid lysis solution is used to lyse the biological sample.

[0070] (1) Place the biological sample to be tested in a 1.5 mL centrifuge tube, add 400 μL of lysis buffer, and add 20 μL of proteinase K and shake well.

[0071] (2) Place at 60°C for 60 min until the sample tissue is completely dissolved and the solution becomes clear;

[0072] (3) Take 200 μL of the lysed solution and add 100 μL of anhydrous ethanol into a centrifuge tube. Gently shake the centrifuge tube for 1 min.

[0073] (4) Add 300 μL of 75 wt% ethanol to the centrifuge tube in step (3), shake it up and down for 30 seconds, then take it out and place it at room temperature to dry;

[0074] (5) Add 100 μL of TE elution buffer to the centrifuge tube in (4) and shake up and down for 1 min to elute the nucleic acid from the device.

[0075] The nucleic acid samples were used to test the nucleic acid adsorption content and A260 / A280 and A260 / A230 using a UV spectrophotometer. Figure 5 ), the centrifuge tube type nucleic acid extraction device prepared by this method has the ability to separate nucleic acids, which proves that the method of preparing nucleic acid extraction devices by using acrylic acid doped quartz sand has high plasticity, and different containers can be used to prepare corresponding nucleic acid extraction devices according to different experimental requirements.

[0076] Example 4

[0077] (1) 2 mL of acrylic acid monomer, 1 mL of crosslinking agent polyethylene glycol diacrylate and 1 g of N'N-methylenebisacrylamide, and 0.001 g of photoinitiator were added to prepare a prepolymer solution;

[0078] (2) The prepolymer solution in step (1) was divided into four groups. The first group did not add quartz sand (PAA), the second group added 2g 120-180 mesh quartz sand (PAA-SiO2-small), another group added 2g 50-80 mesh quartz sand (PAA-SiO2-large), and the last group added 1g 50-80 mesh quartz sand and 1g 120-180 mesh quartz sand (PAA-SiO2-mixed) and stirred evenly;

[0079] (3) After mixing, add (2) to the eight-well tube strip, with 80 μL of the solid solution mixture in each well, and mix well;

[0080] (4) inserting the 3D-printed handle into the eight-tube strip of step (3), placing it under a 300 nm ultraviolet lamp for irradiation and curing for 40 minutes, and performing a free radical polymerization reaction under the illumination condition;

[0081] (5) Take out the eight-row tube in (4), invert and irradiate the bottom for 40 min, and irradiate the side for 40 min;

[0082] (6) Use the handle to demould the device from the eight-row tube and wash it three times with deionized water and anhydrous ethanol; (7) Dry it at 25°C for 1 hour and store it away from light for future use.

[0083] The obtained nucleic acid extraction device is used to extract nucleic acid from a mixed biological sample, and the biological sample is lysed using a self-prepared lysis solution.

[0084] (1) Place the biological sample to be tested in a 1.5 mL centrifuge tube, add 400 μL of lysis buffer, and add 20 μL of proteinase K and shake well.

[0085] (2) Place at 56°C for 40 min until the biological sample tissue is completely dissolved and the solution becomes clear;

[0086] (3) Take 100 μL of the lysed solution and add 60 μL of isopropanol into an eight-tube strip;

[0087] (4) Insert the device into the eight-row tube and gently shake it up and down for 1 min before taking it out;

[0088] (5) Take a new 0.2 mL eight-tube strip and add 150 μL of 75 wt% ethanol. Add the device with adsorbed nucleic acid into the strip and gently shake it up and down for 30 seconds. Then take it out and place it at room temperature to dry.

[0089] (6) Take a new eight-tube strip, add 80 μL of TE elution solution and the dried device, and shake it up and down for 1 min to elute the nucleic acid from the device.

[0090] The nucleic acid samples were used to test the nucleic acid adsorption content and A260 / A280 and A260 / A230 using a UV spectrophotometer. Figure 6 ), the four groups of nucleic acid extraction devices prepared by this method have the ability to separate nucleic acids. The nucleic acid extraction effect of the material doped with quartz sand is higher than that of the material without doping. The size of the doped particles is not limited and can be optimized and formulated according to the experimental purpose.

[0091] Example 5

[0092] (1) Take 2 mL of acrylic acid monomer and 1 mL of cross-linking agent polyethylene glycol diacrylate, add 0.01 g of photoinitiator, and prepare a prepolymer solution;

[0093] (2) adding 1 g of 50-80 mesh quartz sand and 1 g of 120-180 mesh quartz sand to the prepolymer solution in step (1) and stirring evenly;

[0094] (3) After mixing, add (2) to the eight-well tube strip, with 80 μL of the solid solution mixture in each well, and mix well;

[0095] (4) inserting the eight-row tube in step (3) into the handle, placing it under a 300 nm ultraviolet lamp for irradiation and curing for 3 minutes, and performing a free radical polymerization reaction under the light condition;

[0096] (5) Take out the eight-row tubes in (4) and divide them into four groups. The first group is inverted and irradiated for curing at the bottom for 2 minutes and irradiated for curing at the side for 2 minutes. The second group is only irradiated for curing at the bottom for 2 minutes. The third group is only irradiated for curing at the side for 2 minutes. The fourth group is not irradiated for curing at the bottom and side.

[0097] (6) Hold the handle to demold the device from the centrifuge tube, and wash it three times with deionized water and anhydrous ethanol; (7) Dry it at 25°C for 24 hours and store it away from light for future use.

[0098] By comparison, the nucleic acid extraction devices in the four groups were all complete, indicating that this experimental method does not require professionals to operate, has high operational flexibility, and is suitable for the rapid construction of devices in low-resource environments.

Claims

1. A method for preparing a hydrogel nucleic acid separation device, characterized in that: Here are the steps: (1) adding a crosslinking agent and a photoinitiator to a hydrogel monomer to prepare a prepolymer solution; (2) After the prepolymer solution is mixed, it is added to the mold, the handle is inserted, and the mold is placed under a UV lamp for irradiation and curing, and a free radical polymerization reaction is carried out under the light condition to obtain a device; (3) Hold the handle to remove the device from the mold, wash and dry the device, and then store it.

2. The preparation method according to claim 1, characterized in that: The hydrogel monomer is acrylic acid or acrylic ester.

3. The preparation method according to claim 1, characterized in that: The crosslinking agent is one or more of N'N methylene bisacrylamide and polyethylene glycol diacrylate, and the amount of the crosslinking agent is 2-50wt% of the hydrogel monomer.

4. The preparation method according to claim 1, characterized in that: The initiator is (2,4,6-trimethylbenzoyl) diphenylphosphine oxide, and the added amount is 0.1-0.5wt% of the hydrogel monomer.

5. The preparation method according to claim 1, characterized in that: Silica particles are also added to the prepolymer solution; the silica particles are single component silica or other substances with silica as the main component.

6. The preparation method according to claim 1, characterized in that: The silicon dioxide particles are quartz sand particles with a particle size of 50 meshes to 180 meshes, and the added amount of the silicon dioxide particles is 20-50wt% of the solid-liquid mixture.

7. The preparation method according to claim 1, characterized in that: The free radical polymerization reaction is carried out at room temperature, the wavelength of light is 280-400nm, and the irradiation time is 3-40min.

8. The preparation method according to claim 1, characterized in that: The mold is a container with a cavity that matches the needs of DNA extraction, such as a centrifuge tube, an eight-well tube strip, a 96-well plate, and a deep-well plate.

9. The preparation method according to claim 1, characterized in that: The handle is rod-shaped.

10. The preparation method according to claim 1, characterized in that: The device is washed with water and / or ethanol as the washing liquid, and the washing times are not less than once; the device is dried at a temperature of 0-80°, and the drying time is 3min-24h; and the storage conditions are room temperature and away from light.

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