Application of compound 5-ALA and GABA in fertilizers with different functions

Abstract

The invention belongs to application of collocation synergism of agricultural biostimulant in fertilizer, and particularly relates to a composite synergism technology containing 5-ALA and GABA (gamma-aminobutyric acid), and the effective components are 5-ALA and GABA; the weight percentage of the 5-ALA in the composition is 0.0075%-1%, and the weight percentage of the GABA in the composition is 0.1%-5%. According to the invention, 5-ALA and GABA are compounded according to a combination ratio to prepare the functional fertilizer. On the other hand, the activity is greatly improved compared with that of a single agent. Field trials prove that the composition disclosed by the invention has good growth promoting and yield increasing effects on crops.

Description

Technical Field

[0001] The invention belongs to the technical field of preparation of agricultural plant growth regulators, and particularly relates to a composition containing 5-ALA and GABA. Background Art

[0002] 5-ALA is called 5-aminolevulinic acid, and its molecular formula is C5H9N03. 5-ALA is a key precursor for the biosynthesis of all porphyrin compounds, and is widely found in bacteria, fungi, animals and plants. It is a potential physiologically active substance. Studies have shown that ALA can enhance the ability of plants to resist adversity by regulating the permeability of plasma membranes, the activity of membrane proteins and the growth of the root system. It can also enhance the tolerance of plants to hypoxia stress and promote plant growth by promoting the synthesis of chlorophyll and regulating plant respiration. It has a physiological effect similar to that of plant growth regulators, stimulates plant growth and development during cultivation, and can be used in agricultural production.

[0003] GABA, also known as γ-aminobutyric acid, has a molecular formula of C4H9NO2. GABA is a four-carbon non-protein amino acid commonly found in organisms. It can promote the absorption of ions by plants and promote plant growth through interactions with plant hormones. It can also participate in the response to adverse stress by participating in signal transmission, improving the activity of antioxidant enzyme systems, increasing photosynthetic efficiency, and maintaining the carbon-nitrogen balance of plants. It has an outstanding effect in improving plant stress resistance. GABA is not only a signal substance involved in plant stress response, but also a physiological metabolic regulator and nutrient. It can be used as a plant growth regulator in agricultural production.

[0004] No one has reported yet on the combination of the two regulators and their application. Summary of the invention

[0005] In view of the existing technical problems, the purpose of the present invention is to provide a method for using composite 5-ALA and GABA in different functional fertilizers, wherein the active ingredients are 5-ALA and GABA, and the compound combination ratio of 5-ALAH and GABA in different functional fertilizers is controlled, which has an obvious synergistic effect. Field tests have shown that the composition of the present invention has a good growth-promoting and yield-increasing effect on wheat and corn.

[0006] The technical solution of the present invention is as follows: a method for using composite 5-ALA and GABA in different functional fertilizers, wherein the active ingredients are 5-ALA and GABA, the weight percentage of 5-ALA in the composition is 0.0075% to 1%, and the weight percentage of GABA in the composition is 0.1% to 5%.

[0007] Preferably, the mass ratio of the active ingredients 5-ALA and GABA is 3:2000 to 10:1.

[0008] Preferably, liquid or powdered 5-ALA and GABA dosage forms can be selected according to the special needs of functional fertilizers.

[0009] The compound 5-ALA and GABA provided by the present invention can be used for reducing weight and increasing yield of wheat and corn. The functional fertilizer using this technology can be water-soluble fertilizer for root application, foliar fertilizer, and buried fertilizer. According to the type, this fertilizer can be chemical fertilizer, organic fertilizer, or microbial fertilizer.

[0010] Beneficial effects: The present invention compounds 5-ALA and GABA in a certain ratio, and the activity of the compound use is greatly improved compared with the activity of a single dosage form, and has great development value compared with a single dosage form. Experiments have found that the combination of 5-ALA and GABA has a significant effect on increasing the stem thickness and plant height of peppers; under low temperature conditions, the combination of 5-ALA and GABA still has a significant effect on increasing the fresh weight and dry weight of the aboveground part of corn, and significantly promotes the dry matter accumulation of corn seedlings; it has a significant weight-loss effect on the growth cycle of peppers, wheat, and corn, and can increase the yield of crops such as wheat and corn. Implementation

[0011] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0012] In the following examples, percentages are by weight. Example

[0013] 1.1% 5-ALA • GABA compound fertilizer 1% 5-ALA stock solution and 0.1% GABA stock solution are added into compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 1.1% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0014] Example 2 0.85%5-ALA • GABA 0.75% 5-ALA stock solution and 0.1% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 0.85% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0015] Example 3 0.6%5-ALA • GABA 0.5% 5-ALA stock solution and 0.1% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 1.1% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0016] Example 4 0.35%5-ALA • GABA 0.25% 5-ALA stock solution and 0.1% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 0.35% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0017] Example 5 0.125% 5-ALA • GABA 0.025% 5-ALA stock solution and 0.1% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 0.125% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0018] Example 6 0.1075%5-ALA • GABA 0.0075% 5-ALA stock solution and 0.1% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 0.1075% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0019] Example 7 1.25% 5-ALA • GABA 0.25% 5-ALA stock solution and 1% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 1.25% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0020] Example 8 2.25% 5-ALA • GABA 0.25% 5-ALA stock solution and 2% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 2.25% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0021] Example 9 3.25% 5-ALA • % GABA 0.25% 5-ALA stock solution and 3% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 3.25% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0022] Example 10 4.25% 5-ALA • GABA 0.25% 5-ALA stock solution and 4% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to obtain 4.25% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0023] Embodiment 11 5.25% 5-ALA • GABA 0.25% 5-ALA stock solution and 5% GABA stock solution are added to the compound fertilizer / water-soluble fertilizer according to weight percentage and mixed evenly to prepare 5.25% 5-ALA • GABA compound fertilizer / water-soluble fertilizer.

[0024] 1. Indoor potted plant efficiency test 1.1 Indoor potted pepper seedlings efficiency test Crops were treated with combinations and single agents of different proportions and contents, and the rationality of the mixture was measured by measuring the stem thickness, plant height and other traits of the crops in the later stage compared with the blank control area.

[0025] Different concentrations of 5-ALA, GABA and water-soluble fertilizer were combined to test pepper seedlings in pots. The pepper seedlings were fertilized once every 6 days, for a total of three fertilization treatments. Implementation Examples 1-10 were used in the same way, the blank group was treated with water, and the pepper seedlings were measured six days after the third treatment. The test results are shown in Table 1: Table 1: Effects of 5-ALA and GABA on pepper seedlings: deal with Stem diameter (cm) Stem diameter growth rate (%) Plant height (cm) Plant height growth rate (%) 5-ALA 2.66 39.85% 9.77 27.02% GABA 2.62 38.94% 9.86 27.69% 5-ALA:GABA(1%:0.1%) 3.40 52.94% 11.72 39.16% 5-ALA:GABA (0.75%:0.1%) 3.40 52.94% 11.70 39.06% 5-ALA:GABA(0.5%:0.1%) 3.38 52.66% 11.60 38.53% 5-ALA:GABA (0.25%:0.1%) 3.42 53.22% 11.86 39.88% 5-ALA:GABA(0.025%:0.1%) 3.30 51.51% 11.56 38.32% 5-ALA:GABA(0.0075%:0.1%) 3.27 51.07% 11.43 37.62% 5-ALA:GABA (0.25%:1%) 3.21 50.16% 11.47 37.84% 5-ALA:GABA (0.25%:2%) 3.10 48.39% 11.40 37.46% 5-ALA:GABA (0.25%:3%) 3.15 49.20% 11.48 37.89% 5-ALA:GABA (0.25%:4%) 3.12 48.72% 11.25 36.62% 5-ALA:GABA (0.25%:5%) 3.13 48.88% 11.23 36.50% CK 1.60 / 7.13 / Through the indoor pepper seedling physiological index test, it was found that the combination of 5-ALA and GABA had a significant effect on increasing the stem thickness and plant height of pepper, indicating that it significantly promoted the growth of pepper seedlings, and the promotion effect of the combination was better than that of a single agent, and all indicators were better than the blank control. It can be seen that the combination of 5-ALA and GABA has a good synergistic effect on the growth of pepper seedlings.

[0026] 1.2 Experiment on low temperature enhancement of indoor potted corn seedlings Different concentrations of 5-ALA, GABA and water-soluble fertilizer were combined to test potted corn. Corn seeds were sown in pots, and thinned out after 6 days. Five corn seedlings were kept in each pot. Water-soluble fertilizer was applied to the roots once when the corn seedlings had three leaves and one heart. After 24 hours, low-temperature treatment was carried out for 8 days. Implementation Examples 1-10 were used in the same way. The blank group was treated with water. The fresh and dry weight of the aboveground part and other data were measured 9 days after the application of the medicine, that is, 8 days after the low-temperature treatment. The low-temperature conditions are: day / night temperature 14 / 5℃, light time 12h, relative humidity 55%. The experimental results are shown in Table 2: Table 2, Effects of 5-ALA and GABA on corn seedlings: deal with Average fresh weight of above-ground part (g) Fresh weight growth rate (%) Average dry weight of aboveground part (g) Dry weight growth rate (%) 5-ALA 22.22 9.27% 2.73 3.30% GABA 21.13 4.60% 2.71 2.58% 5-ALA:GABA(1%:0.1%) 23.47 14.10% 2.94 10.20% 5-ALA:GABA (0.75%:0.1%) 23.99 15.96% 2.76 4.35% 5-ALA:GABA(0.5%:0.1%) 25.00 19.36% 3.25 18.77% 5-ALA:GABA (0.25%:0.1%) 26.53 24.01% 3.22 18.01% 5-ALA:GABA(0.025%:0.1%) 26.16 22.94% 3.66 27.87% 5-ALA:GABA(0.0075%:0.1%) 25.69 21.53% 3.24 18.52% 5-ALA:GABA (0.25%:1%) 24.18 16.63% 3.14 15.92% 5-ALA:GABA (0.25%:2%) 23.89 15.61% 3.01 12.29% 5-ALA:GABA (0.25%:3%) 23.25 13.29% 3.04 13.16% 5-ALA:GABA (0.25%:4%) 23.05 12.54% 3.09 14.56% 5-ALA:GABA (0.25%:5%) 22.74 11.35% 2.92 9.59% CK 20.16 / 2.64 / Through indoor corn physiological index tests, it was found that under low temperature conditions, the combination of 5-ALA and GABA still had a significant increase in the fresh weight and dry weight of the aboveground part of corn, indicating that it significantly promoted the dry matter accumulation of corn seedlings, and the promotion effect of the combination was better than that of a single agent, and all indicators were better than the blank control. It can be seen that the combination of 5-ALA and GABA has a good synergistic effect on the growth of corn seedlings.

[0027] 2. Field efficacy verification test of the composition of the present invention 2.1 Pepper efficacy test The experiment was arranged to be carried out in the test field in Nanning City, Guangxi Province. The pepper variety tested was Shenglong. No pesticides had been applied to the planting area for some time before. The experiment set up four treatments, each with four replicates. The pepper was transplanted on May 13, 2022, and the first medication date was July 2, 2022. The water-soluble fertilizer with 0.0075% 5-ALA was used as the control group, and the test group was treated with Example 6 (1.25% 5-ALA•GABA water-soluble fertilizer). Each root application was treated, and the blank group was treated with water. The second medication time was July 26, 2022, and the third medication time was August 16, 2022. The yield was measured on August 30, 2022. The yield was measured strictly in accordance with the method specified in the National Standard of the People's Republic of China "Guidelines for Field Tests of Pesticide Efficacy".

[0028] The yield increase rates of the control group and the experimental group were 27.66% and 41.35% respectively. The experiment showed that 1.25% 5-ALA•GABA water-soluble fertilizer had a good yield-increasing effect on pepper.

[0029] 2.2 Corn efficacy test The experiment was arranged to be carried out in the test field in Nanning City, Guangxi Province. The corn variety for the test was Xianyu. No pesticides had been applied to the planting area for some time before. The experiment had four treatments, and each treatment was repeated four times. The corn was sown on March 25, 2022, and the first application date was May 25, 2022. The compound fertilizer with 0.0075% 5-ALA was added as the control group. The test group was treated with Example 6 (1.25% 5-ALA•GABA compound fertilizer). Each root application was treated, and the blank group was treated with water. The second application time was June 8, 2022, and the third application time was June 22, 2022. The yield was measured on July 22, 2022. The yield was measured strictly in accordance with the method specified in the National Standard of the People's Republic of China "Guidelines for Field Tests of Pesticide Efficacy".

[0030] The yield increase rates of the control group and experimental group were 5.41% and 13.20% respectively.

[0031] Experiments show that 1.25% 5-ALA•GABA compound fertilizer has a good yield-increasing effect on corn.

[0032] Indoor bioassay tests and outdoor field comparative tests have shown that the compound of 5-ALA and GABA has a good promoting effect on the growth of peppers and corn, and is a pesticide variety worthy of promotion and use.

Claims

1. A functional fertilizer using 5-ALA and GABA composite synergistic technology, characterized in that: According to the physical form, this fertilizer can be powder fertilizer, granular fertilizer, clear liquid, suspended fertilizer. According to the method of use, this fertilizer can be root fertilizer, foliar fertilizer, and buried fertilizer. According to the type, this fertilizer can be chemical fertilizer, organic fertilizer, and microbial fertilizer.

2. The functional fertilizer using the 5-ALA and GABA composite synergistic technology as claimed in claim 1, characterized in that: The synergistic use technology of 5-ALA and GABA, the weight percentage of 5-ALA in the composition is 0.0075%~1%, and the weight percentage of GABA in the composition is 0.1%~5%.