Weight-losing functional beverage based on koumiss extract and preparation method thereof

Abstract

The invention relates to the technical field of functional drinks, and particularly discloses a functional drink for losing weight based on a koumiss extract and a preparation method thereof.The preparation method comprises the steps that S1, the koumiss extract contains at least one key component with the weight losing effect; s2, preparing a nano-scale carrier system, wherein the nano-scale carrier system is used for packaging key components in the koumiss extract; according to the invention, key components in the koumiss are extracted to obtain the koumiss extract, so that the key components with the weight-losing effect are efficiently extracted from the koumiss, and the components comprise but are not limited to probiotics, active peptides, lactic acid, short-chain fatty acid and the like; the components have remarkable effects in the aspects of promoting digestion, regulating intestinal flora, accelerating fat metabolism and the like and are core elements for forming the weight-losing functional beverage, high purity and high activity of key components in the beverage are ensured through accurate extraction, a solid foundation is laid for the subsequent preparation process, and meanwhile the weight-losing effect and safety of the beverage are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of functional drinks, and particularly relates to a weight-loss functional drink based on yogurt extract and a preparation method thereof. Background Art

[0002] With the accelerated pace of modern life and changes in dietary structure, obesity has become a global health challenge. In order to meet this challenge, various weight loss products and functional drinks have emerged on the market. Among them, weight loss functional drinks based on natural ingredients have attracted much attention due to their safety and effectiveness. Sour mare's milk, as a traditional fermented dairy product, is widely believed by the people to have health benefits such as promoting digestion and regulating intestinal flora. In recent years, its potential weight loss effect has gradually received attention from scientific research.

[0003] A variety of weight loss functional drinks based on natural ingredients have been developed. These drinks usually achieve weight loss effects by extracting active substances from plant, fruit or animal-derived ingredients, such as polyphenols, dietary fiber, probiotics, etc. However, although yogurt has many health benefits and weight loss potential, it still faces many challenges in the process of developing it into a functional drink. The extraction efficiency and stability of key ingredients in yogurt are difficult to ensure, and traditional extraction methods often have problems such as low efficiency and large ingredient loss, resulting in insufficient content of effective ingredients in the final product, affecting the weight loss effect of the drink, so staff need to improve it. Summary of the invention

[0004] The object of the present invention is to provide a weight loss functional drink based on sour mare's milk extract and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A weight loss functional drink based on sour mare's milk extract, comprising:

[0007] S1, a sour mare's milk extract comprising at least one key ingredient having a weight loss effect;

[0008] S2, a nanoscale carrier system, wherein the nanoscale carrier system is used to encapsulate the key components in the yogurt extract, wherein the nanoscale carrier system is made of a biocompatible material, and the particle size thereof is optimized to improve the solubility and stability of the key components in the gastrointestinal tract;

[0009] S3. The nanoscale carrier system significantly improves the absorption rate and biological activity of the key ingredients.

[0010] Preferably, the nanoscale carrier system is made of one or a combination of the following materials: liposomes, polymer nanoparticles, inorganic nanoparticles, and modified forms thereof.

[0011] Preferably, the nanoscale carrier system has intelligent responsiveness and can release the key ingredients under specific gastrointestinal environmental conditions.

[0012] Preferably, the intelligent responsiveness is based on changes in pH, temperature and enzyme activity.

[0013] Preferably, the key components in the yogurt extract include but are not limited to probiotics, active peptides, lactic acid, short-chain fatty acids and combinations thereof.

[0014] A method for preparing a weight loss functional drink based on yogurt extract comprises the following steps:

[0015] a. extracting the key components in yogurt to obtain yogurt extract;

[0016] b. preparing precursor materials for nanoscale carrier systems;

[0017] c. mixing the yogurt extract with the precursor material of the nanoscale carrier system and combining it with one of emulsification, solvent evaporation, self-assembly and supercritical fluid technology to form nanoparticles encapsulating the key ingredients;

[0018] d. characterizing the nanoparticles to ensure that their particle size, encapsulation efficiency and stability meet predetermined requirements, and the characterization process includes using a method that can quantify the parameters in the formula;

[0019] e. Mixing the nanoparticles with a suitable beverage matrix to form a weight loss functional beverage.

[0020] Preferably, the nanoparticles are subjected to a surface modification step to enhance their stability and biocompatibility in the gastrointestinal tract, and the surface modification process is intended to further optimize the parameters in the formula.

[0021] Preferably, the characterization step comprises using one or a combination of dynamic light scattering, scanning electron microscopy, transmission electron microscopy and centrifugal dialysis methods to quantify the particle size, encapsulation efficiency and stability of the nanoparticles, and optimize the parameters in the formula accordingly.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) By extracting key components from yogurt, yogurt extract is obtained, and the key components with weight loss effect are efficiently extracted from yogurt. These components include but are not limited to probiotics, active peptides, lactic acid, short-chain fatty acids, etc. These components have significant effects on promoting digestion, regulating intestinal flora, accelerating fat metabolism, etc., and are the core elements of weight loss functional drinks. Through precise extraction, the high purity and high activity of the key components in the drink are ensured, which lays a solid foundation for the subsequent preparation process and improves the weight loss effect and safety of the drink.

[0024] (2) Through the precursor material of the nanoscale carrier system and the encapsulation of key ingredients to form nanoparticles, it is possible to use biocompatible materials such as liposomes and polymer nanoparticles to construct a nanoscale carrier system and encapsulate the key ingredients in the yogurt extract. The nanoscale carrier system can significantly improve the solubility and stability of the key ingredients in the gastrointestinal tract, while improving their absorption rate and biological activity. Through encapsulation, it not only protects the key ingredients from damage by the gastrointestinal environment, but also achieves its precise release under specific conditions, thereby improving the weight loss effect of the drink and prolonging the action time of the key ingredients in the gastrointestinal tract. In addition, the intelligent responsiveness design of the nanoscale carrier system enables it to respond more accurately to changes in pH, temperature and enzyme activity, further enhancing the pertinence and effectiveness of the drink.

[0025] (3) By characterizing the nanoparticles and preparing them into functional drinks for weight loss, quantitative analysis of the particle size, encapsulation efficiency and stability of the nanoparticles encapsulating the key ingredients was achieved to ensure that they met the predetermined requirements. The characterization process used advanced equipment such as dynamic light scattering and scanning electron microscopy, which can accurately evaluate the performance of the nanoparticles and provide a reliable basis for the subsequent preparation process. Through characterization, the high quality and stability of the nanoparticles were ensured, providing a strong guarantee for the preparation of drinks with excellent weight loss effects. At the same time, adding appropriate amounts of natural sweeteners, acidulants and flavors during the preparation process not only improves the taste and flavor of the drink, but also ensures its healthiness and safety. These flavorings are all natural ingredients, and the amount added is strictly controlled within a safe range and will not have a negative impact on the weight loss effect of the drink. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The figure is a flow chart of the method of the present invention. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Embodiment 1:

[0029] See also Figure 1 As shown, a weight loss functional drink based on sour mare's milk extract comprises:

[0030] S1, a sour mare's milk extract comprising at least one key ingredient having a weight loss effect;

[0031] S2. Nanoscale carrier system, which is used to encapsulate the key components in the yogurt extract, wherein the nanoscale carrier system is made of biocompatible materials and its particle size is optimized to improve the solubility and stability of the key components in the gastrointestinal tract:

[0032]

[0033] E bio-eff (E_bio-eff is the corresponding character) is the bioeffectiveness enhancement factor, which indicates the comprehensive enhancement effect of the nanoscale carrier system on the biological activity of the key components.

[0034] t max (t_max is the corresponding character) is the maximum time of action of the key components in the gastrointestinal tract.

[0035] C i (t)(C_i(t) is the corresponding character) is the concentration of the i-th key component at time t.

[0036] Sol i (t) (Sol_i(t) is the corresponding character) is the solubility function of the i-th key component in the gastrointestinal tract at time t, in the form of a Sigmoid function, such as Where k1 and t0 are parameters.

[0037] Sta i (t) (Sta_i(t) is the corresponding character) is the stability function of the i-th key component in the gastrointestinal tract at time t, which takes the form of an exponential decay function, such as Where is the k2 parameter.

[0038] λ (lambda is the corresponding character) is the attenuation coefficient, which indicates the decay rate of the key component in the gastrointestinal tract over time.

[0039] (C_i^ref(t) is the corresponding character) is the reference concentration of the i-th key component without using the nanoscale carrier system at time t.

[0040] Abs nano (Abs_nano is the corresponding character) is the absorption rate of the key ingredient after using the nanoscale carrier system.

[0041] Abs ref (Abs_ref is the corresponding character) is the reference absorbance of the key component without using the nanoscale carrier system.

[0042] S3. Among them, the nanoscale carrier system significantly improves the absorption rate and biological activity of key ingredients.

[0043] The nanoscale carrier system is made of one or a combination of the following materials: liposomes, polymer nanoparticles, inorganic nanoparticles, and modified forms thereof.

[0044] Through the optimized extraction process, the key ingredients with weight loss effect are efficiently extracted from the sour mare's milk, ensuring the high purity and high activity of the key ingredients in the drink. This step is the basis for preparing weight loss functional drinks and provides high-quality raw materials for subsequent steps.

[0045] The composite form of liposomes and polymer nanoparticles is used to prepare a nanoscale carrier system with high encapsulation efficiency and stability. The system can accurately respond to changes in the gastrointestinal environment and release key ingredients, thereby improving their bioavailability and weight loss effect.

[0046] The nanoscale carrier system has intelligent responsiveness and is able to release key ingredients under specific gastrointestinal environmental conditions. The intelligent responsiveness is designed and optimized based on the following formula:

[0047] R release (t) = ∫(E env (t),P nano );

[0048] Among them, R release (t) represents the release rate at time t, E env (t) represents the changes in gastrointestinal environmental conditions (such as pH, temperature, and enzyme activity) over time, P nano Represents the physical and chemical properties of nanoscale carrier systems.

[0049] Smart responsiveness is based on changes in pH, temperature and enzyme activity, which are quantified by the following formula:

[0050] E env (t)=α·pH(t)+β·T(t)+γ·Eenz (t);

[0051] Among them, α, β and γ are weight coefficients, pH(t), T(t) and E enz (t) represent the pH value, temperature and enzyme activity at time t, respectively.

[0052] The key components in the yogurt extract include but are not limited to probiotics, active peptides, lactic acid, short-chain fatty acids and combinations thereof. The biological activity of the key components is quantified by the following formula:

[0053] A bio (C) = k·Cδ;

[0054] Among them, A bio (C) represents biological activity, C represents the concentration of the key component, and k and δ are constants.

[0055] A method for preparing a weight loss functional drink based on yogurt extract comprises the following steps:

[0056] a. extracting the key components in yogurt to obtain yogurt extract;

[0057] b. preparing precursor materials for nanoscale carrier systems;

[0058] c. mixing the yogurt extract with the precursor material of the nanoscale carrier system and combining it with one of emulsification, solvent evaporation, self-assembly and supercritical fluid technology to form nanoparticles encapsulating the key ingredients;

[0059] d. Characterize the nanoparticles to ensure that their particle size, encapsulation efficiency and stability meet the predetermined requirements, and the characterization process includes the use of methods that can quantify the parameters in the formula;

[0060] e. Mix the nanoparticles with a suitable beverage matrix to form a weight loss functional beverage.

[0061] The nanoparticles were subjected to a surface modification step to enhance their stability and biocompatibility in the gastrointestinal tract, and the surface modification process was designed to further optimize the parameters in the formula.

[0062] The characterization step includes using one or a combination of dynamic light scattering, scanning electron microscopy, transmission electron microscopy and centrifugal dialysis methods to quantify the particle size, encapsulation efficiency and stability of the nanoparticles, and optimize the parameters in the formula accordingly.

[0063] Advanced equipment such as dynamic light scattering and scanning electron microscopy are used to characterize nanoparticles and quantify parameters such as particle size, encapsulation efficiency and stability. The characterization results are used to further optimize the parameters in the preparation process to ensure the high quality and stability of nanoparticles.

[0064] Embodiment 2:

[0065] See also Figure 1 As shown, the preparation and smart responsiveness of the nanoscale carrier system were optimized.

[0066] Specifically, the nanoscale carrier system uses a composite form of liposomes and polymer nanoparticles to improve encapsulation efficiency and stability. At the same time, the intelligent responsive design further considers the complex environment of the gastrointestinal tract. By adjusting the ratio of liposomes and polymer nanoparticles and surface modification, the nanoscale carrier system can more accurately respond to changes in pH, temperature and enzyme activity, thereby releasing key ingredients.

[0067] A weight loss functional drink based on sour mare's milk extract, the preparation method of which comprises the following steps:

[0068] The key components in sour mare's milk are extracted and optimized extraction process is adopted to improve the purity and activity of the key components.

[0069] The precursor materials of the nanoscale carrier system are prepared in the form of a composite of liposomes and polymer nanoparticles.

[0070] The yogurt extract is mixed with the precursor material of the nanoscale carrier system, and nanoparticles encapsulating the key ingredients are formed through emulsification, solvent volatilization and other technologies.

[0071] Nanoparticles are characterized using advanced equipment such as high-resolution mass spectrometers to ensure that their particle size, encapsulation efficiency and stability meet the predetermined requirements.

[0072] The nanoparticles are mixed with a suitable beverage matrix and a proper amount of natural flavoring agent is added to form a weight loss functional beverage.

[0073] Embodiment three:

[0074] See also Figure 1 As shown in the figure, when preparing the weight loss functional drink, in addition to mixing the nanoparticles with the drink matrix, an appropriate amount of natural sweeteners, acidulants, flavors and other flavoring agents are added to improve the taste and flavor of the drink. At the same time, in order to ensure the healthiness of the drink, the selected flavoring agents are all natural ingredients, and the amount added is strictly controlled within a safe range.

[0075] In addition, new characterization methods were employed, such as the use of high-resolution mass spectrometry to precisely analyze the composition of the nanoparticles to ensure that they met predetermined quality standards.

[0076] Natural sweeteners such as oligofructose and monk fruit extract are used to replace traditional chemical sweeteners to ensure the healthiness of beverages.

[0077] Add appropriate amount of natural acidulants such as citric acid, malic acid, etc. to adjust the taste of the drink.

[0078] Choose natural flavors with unique flavors, such as strawberry flavor, lemon flavor, etc., to enrich the taste and flavor of the drink.

[0079] When adding flavorings, strictly control the amount added to ensure that it does not negatively affect the weight loss effect of the drink.

[0080] Add appropriate amounts of natural sweeteners, acidulants, essences and other flavorings to the beverage to improve the taste and flavor of the beverage. These flavorings are all natural ingredients, and the amount added is strictly controlled within a safe range and will not have a negative impact on the weight loss effect of the beverage.

[0081] Embodiment 4:

[0082] See also Figure 1 As shown, first, a sour mare's milk extract is prepared, which is obtained through a specific extraction process and contains key ingredients with weight loss effects such as probiotics, active peptides, lactic acid, and short-chain fatty acids. Subsequently, the precursor material of the nanoscale carrier system is prepared, and the composite form of liposomes and polymer nanoparticles is selected to improve the encapsulation efficiency and stability.

[0083] In the process of preparing nanoparticles, the yogurt extract is mixed with the precursor material of the nanoscale carrier system, and the nanoparticles encapsulating the key ingredients are formed through emulsification, solvent evaporation and other technologies. To further optimize the performance of the nanoparticles, they are surface modified to enhance their stability and biocompatibility in the gastrointestinal tract.

[0084] After the preparation of the nanoparticles is completed, they are characterized and their particle size, encapsulation efficiency and stability are quantitatively analyzed using dynamic light scattering, scanning electron microscopy, transmission electron microscopy and other equipment. After ensuring that the nanoparticles meet the predetermined requirements, they are mixed with a suitable beverage matrix and an appropriate amount of natural sweeteners (such as oligofructose), acidulants (such as citric acid) and flavorings such as flavors are added to improve the taste and flavor of the beverage. At the same time, in order to ensure the healthiness of the beverage, the selected flavorings are all natural ingredients, and the amount added is strictly controlled within a safe range.

[0085] In the comparative test, two common weight loss drinks on the market were selected as the control group, which were recorded as Drink A and Drink B. Drink A uses traditional herbal extracts as the main ingredient, while Drink B is a weight loss drink with chemical synthesis as the main ingredient. In order to more comprehensively evaluate the advantages of the present invention, the following test parameters were designed: bioavailability enhancement factor (E_bio-eff), maximum time of action of key ingredients in the gastrointestinal tract (t_max), absorption rate of key ingredients (Abs_nano), and reference absorption rate of key ingredients without using nanoscale carrier system (Abs_ref).

[0086] The test data table is as follows:

[0087] name E_bio-eff t_max(h) Abs_nano(%) Abs_ref(%) This drink 2.5 6 90 60 Drink A 1.2 4 70 55 Drink B 1.8 5 80 65

[0088] By comparing and analyzing the data in the above tables, we can clearly see the advantages of this drink.

[0089] First, in terms of the bioavailability enhancement factor (E_bio-eff), the present invention reaches 2.5, which is much higher than 1.2 of drink A and 1.8 of drink B. This shows that the bioactivity of the key ingredients in the present drink in the gastrointestinal tract has been significantly improved through the encapsulation of the nanoscale carrier system.

[0090] Secondly, in terms of the maximum time (t_max) of the key ingredients in the gastrointestinal tract, this drink reaches 6 hours, which is also longer than 4 hours of drink A and 5 hours of drink B. This means that the key ingredients in this drink can continue to work in the gastrointestinal tract, thereby more effectively promoting weight loss.

[0091] Furthermore, in terms of the absorption rate of key ingredients (Abs_nano), the present drink reached 90%, while drink A and drink B were 70% and 80% respectively. This shows that the absorption rate of the key ingredients in the present drink has been significantly improved through the encapsulation of the nanoscale carrier system, thereby improving its weight loss effect.

[0092] Finally, in terms of the reference absorption rate (Abs_ref) of the key ingredients without the use of a nanoscale carrier system, although the values ​​of the three drinks are similar, combined with the data of Abs_nano, it can be seen that this drink is more effective in improving the absorption rate.

[0093] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A weight loss functional drink based on yogurt extract, characterized in that: include: S1, a sour mare's milk extract comprising at least one key ingredient having a weight loss effect; S2, a nanoscale carrier system, wherein the nanoscale carrier system is used to encapsulate the key components in the yogurt extract, wherein the nanoscale carrier system is made of a biocompatible material, and the particle size thereof is optimized to improve the solubility and stability of the key components in the gastrointestinal tract; S3. The nanoscale carrier system significantly improves the absorption rate and biological activity of the key ingredients.

2. A weight loss functional drink based on yogurt extract according to claim 1, characterized in that: The nanoscale carrier system is made of one or a combination of the following materials: liposomes, polymer nanoparticles, inorganic nanoparticles and modified forms thereof.

3. The weight loss functional drink based on yogurt extract according to claim 1, characterized in that: The nanoscale carrier system has intelligent responsiveness and can release the key ingredients under specific gastrointestinal environmental conditions.

4. The weight loss functional drink based on yogurt extract according to claim 1, characterized in that: The smart responsiveness is based on changes in pH, temperature and enzyme activity.

5. The weight loss functional drink based on yogurt extract according to claim 1, characterized in that: The key components in the yogurt extract include but are not limited to probiotics, active peptides, lactic acid, short-chain fatty acids and combinations thereof.

6. A method for preparing a weight loss functional drink based on sour mare's milk extract, applicable to the weight loss functional drink based on sour mare's milk extract according to claims 1 to 5, characterized in that: The following steps are involved: a. extracting the key components in yogurt to obtain yogurt extract; b. preparing precursor materials for nanoscale carrier systems; c. mixing the yogurt extract with the precursor material of the nanoscale carrier system and combining it with one of emulsification, solvent evaporation, self-assembly and supercritical fluid technology to form nanoparticles encapsulating the key ingredients; d. characterizing the nanoparticles to ensure that their particle size, encapsulation efficiency and stability meet predetermined requirements, and the characterization process includes using a method that can quantify the parameters in the formula; e. Mixing the nanoparticles with a suitable beverage matrix to form a weight loss functional beverage.

7. The method for preparing a weight loss functional drink based on yogurt extract according to claim 6, characterized in that: The nanoparticles are subjected to a surface modification step to enhance their stability and biocompatibility in the gastrointestinal tract, and the surface modification process is intended to further optimize the parameters in the formula.

8. The method for preparing a weight loss functional drink based on yogurt extract according to claim 6, characterized in that: The characterization step includes using one or a combination of dynamic light scattering, scanning electron microscopy, transmission electron microscopy and centrifugal dialysis methods to quantify the particle size, encapsulation efficiency and stability of the nanoparticles, and optimize the parameters in the formula accordingly.

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