Extraction method and device for high-purity konjac glucomannan

Abstract

The invention relates to the technical field of compound extraction, in particular to an extraction method and device for high-purity konjac glucomannan, and the method comprises the steps of conducting degreasing treatment on a konjac material through an n-hexane solution to obtain a degreased konjac sample, conducting non-sugar degradation on the degreased konjac sample through a degrading enzyme solution to obtain an original glycan solution, and conducting drying to obtain the high-purity konjac glucomannan. Adjusting the pH value of the original glycan solution to obtain a target glycan solution, measuring the mass of the solution, performing gulcomannan precipitation by using a calcium chloride solution to obtain original glycan precipitation and a reduction centrifugal solution, judging whether precipitation exists in the reduction centrifugal solution or not, and if so, performing precipitation separation on the reduction centrifugal solution to obtain a target centrifugal solution; and returning to the step of measuring the mass of the solution, if not, collecting the original glycan precipitate to obtain a target glycan precipitate, and washing and drying to obtain the high-purity konjac glucomannan. According to the method, the extraction purity of the konjac glucomannan can be improved, and the pollution of the extraction waste liquid to the environment is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of compound extraction, and in particular to a method and a device for extracting high-purity konjac glucomannan. Background Art

[0002] The main active ingredient of konjac is konjac glucomannan, which has the characteristics of high swelling, high elasticity and high viscosity, and can significantly lower blood lipids and increase bile acid excretion, reduce fat and cholesterol absorption, and lower blood cholesterol and triglyceride levels. At the same time, konjac glucomannan is an economical and effective natural food preservative that can effectively prevent food from spoiling, mold and insect pests. Therefore, it is particularly important to extract high-purity konjac glucomannan.

[0003] Currently, the extraction method of konjac glucomannan is achieved through ethanol extraction. However, when high-concentration ethanol is used as the extraction solvent, other impurities, such as protein, may not be distinguished. These impurities will precipitate together with the konjac glucomannan, resulting in low purity of the extracted konjac glucomannan. At the same time, the waste liquid from high-concentration ethanol extraction is more harmful to the environment. Summary of the invention

[0004] The invention provides a method and a device for extracting high-purity konjac glucomannan, the main purpose of which is to improve the extraction purity of konjac glucomannan and reduce the pollution of extraction waste liquid to the environment.

[0005] To achieve the above object, the present invention provides a method for extracting high-purity konjac glucomannan, comprising:

[0006] Obtaining a n-hexane solution and a konjac material, and utilizing the n-hexane solution to defat the konjac material to obtain a defatted konjac sample;

[0007] Measuring the defatted sample mass of the defatted konjac sample, preparing a degradation enzyme solution based on the defatted sample mass, and performing non-sugar degradation on the defatted konjac sample using the degradation enzyme solution to obtain an original polysaccharide solution;

[0008] adjusting the pH of the original polysaccharide solution to obtain a target polysaccharide solution;

[0009] measuring the mass of the target polysaccharide solution, obtaining a calcium chloride solution based on the mass of the solution, and using the calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitated solution;

[0010] The original polysaccharide precipitation solution is subjected to precipitation centrifugation separation to obtain an original polysaccharide precipitation and an original centrifugal solution, and the original centrifugal solution is subjected to a calcium ion reduction operation using a preset sodium carbonate solution to obtain a reduced centrifugal solution;

[0011] Determining whether there is precipitation in the reduced centrifugal solution;

[0012] If there is precipitation in the reduced centrifugal solution, the reduced centrifugal solution is subjected to precipitation separation to obtain a target centrifugal solution, the target centrifugal solution is used as a target glycan solution, and the process returns to the step of measuring the solution quality of the target glycan solution;

[0013] If there is no precipitate in the reduced centrifugal solution, the original polysaccharide precipitates are collected to obtain target polysaccharide precipitates, and the target polysaccharide precipitates are washed and dried to obtain high-purity konjac glucomannan.

[0014] Optionally, the konjac material is defatted using n-hexane solution to obtain a defatted konjac sample, comprising:

[0015] The konjac material is crushed and dried to obtain a crushed konjac sample;

[0016] Using a pre-built reactor, the n-hexane solution and the crushed konjac sample are mixed and reacted to obtain a mixed konjac sample;

[0017] Based on the preset stirring and dissolving duration, the mixed konjac sample is stirred to obtain a grease separation sample, wherein the grease separation sample is a mixture of a grease mixed solvent and original defatted konjac;

[0018] Described oil separation sample is carried out solvent filtration operation, obtain original defatted konjac, measure original oil content in described original defatted konjac;

[0019] Determining whether the original oil content is less than a preset target oil content;

[0020] If described original oil content is not less than target oil content, then use original defatted konjac as crushing konjac sample, and return described utilization pre-built reactor, described n-hexane solution and crushing konjac sample are carried out the step of mixed reaction;

[0021] If the original oil content is less than the target oil content, the original defatted konjac is dried to obtain a defatted konjac sample.

[0022] Optionally, the preparation of the degradative enzyme solution based on the mass of the defatted sample comprises:

[0023] According to the mass of the defatted sample and the preset unit mass of enzyme activity, a multi-enzyme mass set is calculated, wherein the unit mass of enzyme activity includes: a unit mass of protease, a unit mass of cellulase, a unit mass of pectinase and a unit mass of mannanase, and the multi-enzyme mass set includes: a mass of protease, a mass of cellulase, a mass of pectinase and a mass of mannanase, and the multi-enzyme mass set is expressed as:

[0024] m={m d ,m x ,m v ,m g}

[0025] m d =m t ×u d , m x =m t ×u x , m v =m t ×u v , m g =m t ×u g

[0026] Where m represents the multi-enzyme mass set, m d Indicates the mass of protease, m t Indicates the mass of the defatted sample, u d Indicates the unit mass of protease, m x Indicates the mass of cellulase, u x Indicates the unit mass of cellulase, m v Indicates the quality of pectinase, u v Indicates the unit mass of pectinase, m g Indicates the mass of glycosidase, u g Indicates the unit mass of glycosidase;

[0027] Weighing protease, cellulase, pectinase and mannanase based on the multi-enzyme mass set, and preparing a target buffer based on protease, cellulase, pectinase and mannanase;

[0028] The protease, cellulase, pectinase and mannanase are added to the target buffer to obtain a degradation enzyme solution.

[0029] Optionally, the target buffer is prepared based on protease, cellulase, pectinase and mannanase, comprising:

[0030] Obtaining the optimal pH ranges of the protease, cellulase, pectinase and mannanase, wherein the optimal pH range is the intersection of the protease pH range, the cellulase pH range, the pectinase pH range and the mannanase pH range;

[0031] Determine a central pH value according to the optimal pH range, wherein the central pH value is the middle value of the optimal pH range;

[0032] According to the central pH value, the preset weak acid concentration and the acid dissociation equilibrium constant, the conjugate base concentration is calculated using the following formula:

[0033]

[0034] Where P represents the central pH value, P k represents the acid dissociation equilibrium constant, lg(*) represents the logarithmic function, C s Indicates the concentration of weak acid, C g represents the conjugate base concentration;

[0035] Prepare a target buffer based on the weak acid concentration and conjugate base concentration.

[0036] Optionally, the non-sugar degradation of the defatted konjac sample using a degrading enzyme solution to obtain an original polysaccharide solution comprises:

[0037] The degradative enzyme solution is mixed with the defatted konjac sample to obtain a degraded konjac solution;

[0038] According to the preset degradation time and degradation cycle, the number of acid-base measurements is set, and according to the number of acid-base measurements, the pH of the degraded konjac solution is measured to obtain a real-time degradation solution and a real-time pH value set, wherein the number of acid-base measurements and the real-time pH value set are respectively expressed as:

[0039]

[0040] Where n represents the number of acid-base measurements, t s represents the degradation time, T represents the degradation cycle, P s Represents the real-time pH value set, Indicates the first real-time pH value, Indicates the nth real-time pH value;

[0041] The real-time variance of the real-time pH value set is calculated using the following formula:

[0042]

[0043] Among them, S represents the real-time variance, represents the real-time pH value of the ith Indicates the jth real-time pH value.

[0044] Determining whether the real-time variance is greater than a preset standard variance;

[0045] If the real-time variance is greater than the standard deviation, the real-time degradation solution is used as the degraded konjac solution, and the step of setting the acid-base measurement number according to the preset degradation duration and degradation cycle is returned;

[0046] If the real-time variance is not greater than the standard variance, the real-time degradation solution is subjected to a high temperature enzyme inactivation treatment to obtain an original polysaccharide solution.

[0047] Optionally, the pH of the original polysaccharide solution is adjusted to obtain the target polysaccharide solution, comprising:

[0048] measuring the original polysaccharide pH value of the original polysaccharide solution;

[0049] Determining whether the original polysaccharide pH value is within a preset polysaccharide pH range;

[0050] If the original polysaccharide pH value is not within the polysaccharide pH range, selecting an acid-base regulator according to the original polysaccharide pH value, and dropping the acid-base regulator into the original polysaccharide solution to obtain an adjusted polysaccharide solution;

[0051] Recording the adjusted polysaccharide solution as the original polysaccharide solution, and returning to the step of measuring the original polysaccharide pH value of the original polysaccharide solution;

[0052] If the original polysaccharide pH value is within the polysaccharide pH range, the original polysaccharide solution is recorded as the target polysaccharide solution.

[0053] Optionally, the step of using a calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitated solution comprises:

[0054] The original solute mass of the target polysaccharide solution is measured, and the single addition volume of the calcium chloride solution is calculated based on the original solute mass and the preset precipitation solution concentration, wherein the single addition volume is expressed as:

[0055]

[0056] Where V represents the single addition volume, m y represents the original solute mass, c d represents the concentration of precipitation solution;

[0057] Based on the single precipitation volume, extracting a unit precipitation reagent from the calcium chloride solution, and adding the unit precipitation reagent to the target polysaccharide solution to obtain a precipitation reaction solution;

[0058] Based on the preset alcohol volume, pre-obtained industrial alcohol is added to the precipitation reaction solution to obtain an original polysaccharide precipitation solution.

[0059] Optionally, the subjecting the original polysaccharide precipitation solution to precipitation centrifugation to obtain the original polysaccharide precipitation and the original centrifugation solution comprises:

[0060] Transferring the original glycan precipitate to a pre-constructed centrifuge tube cluster to obtain a plurality of original solutions to be separated, wherein the centrifuge tube cluster is placed on a plurality of centrifuge radius circles of a centrifuge rotor;

[0061] Performing a solution balancing operation on the multiple original solutions to be separated to obtain multiple target solutions to be separated, wherein the solution balancing operation refers to balancing the masses of the multiple original solutions to be separated;

[0062] Measuring the distances between the bottoms of the plurality of centrifuge tubes and the center of the centrifuge rotor to obtain a plurality of centrifugal distances, and calculating an average centrifugal distance of the plurality of centrifugal distances;

[0063] The rotor speed is determined according to the average centrifugal distance and the preset relative centrifugal force, wherein the rotor speed is expressed as:

[0064]

[0065] Among them, r represents the rotor speed, R represents the relative centrifugal force, and l represents the average centrifugal distance;

[0066] According to the rotor speed and the preset separation time, the plurality of target solutions to be separated are centrifuged to obtain a plurality of target separation precipitates and a plurality of target separation solutions;

[0067] The multiple target separation precipitates and the multiple target separation solutions are respectively combined to obtain an original glycan precipitate and an original centrifugation solution.

[0068] Optionally, the target polysaccharide precipitate is washed and dried to obtain high-purity konjac glucomannan, comprising:

[0069] Measuring a target precipitation volume of the target glycan precipitation, and obtaining high-concentration ethanol based on the target precipitation volume, wherein the ethanol concentration of the high-concentration ethanol is greater than 95%;

[0070] adding the high concentration ethanol to the target polysaccharide precipitate to obtain a washed polysaccharide precipitate, and contacting the washed polysaccharide precipitate with ethanol to obtain a precipitate ethanol mixture;

[0071] According to a preset static time, the impurities of the precipitation ethanol mixture are dissolved to obtain a dissolved mixture, and the dissolved mixture is centrifuged to obtain a precipitation sedimentation mixture, wherein the precipitation sedimentation mixture comprises: an ethanol clear solution and a sedimentation substrate;

[0072] The ethanol clear liquid in the precipitation and sedimentation mixture is removed to obtain a sedimentation substrate, and the sedimentation substrate is washed and dried with the pre-obtained anhydrous ethanol to obtain high-purity konjac glucomannan.

[0073] To achieve the above object, the present invention also provides an extraction device for high-purity konjac glucomannan, comprising:

[0074] The original polysaccharide collection module is used to obtain a n-hexane solution and a konjac material, perform a defatting treatment on the konjac material using the n-hexane solution to obtain a defatted konjac sample, measure the defatted sample mass of the defatted konjac sample, prepare a degradative enzyme solution based on the defatted sample mass, and perform non-sugar degradation on the defatted konjac sample using the degradative enzyme solution to obtain an original polysaccharide solution;

[0075] a polysaccharide precipitation generation module, which is used to adjust the pH of the original polysaccharide solution to obtain a target polysaccharide solution, measure the solution mass of the target polysaccharide solution, obtain a calcium chloride solution based on the solution mass, and use the calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitation solution;

[0076] A reduction solution acquisition module is used to perform precipitation centrifugation separation on the original polysaccharide precipitation solution to obtain original polysaccharide precipitation and original centrifugal solution, and a preset sodium carbonate solution is used to perform a calcium ion reduction operation on the original centrifugal solution to obtain a reduction centrifugal solution;

[0077] The glucomannan preparation module is used to determine whether there is precipitation in the reduced centrifugal solution. If there is precipitation in the reduced centrifugal solution, the reduced centrifugal solution is subjected to precipitation separation to obtain a target centrifugal solution, the target centrifugal solution is used as a target polysaccharide solution, and the step of measuring the solution quality of the target polysaccharide solution is returned. If there is no precipitation in the reduced centrifugal solution, the original polysaccharide precipitation is collected to obtain a target polysaccharide precipitation, and the target polysaccharide precipitation is washed and dried to obtain high-purity konjac glucomannan.

[0078] In order to solve the above problem, the present invention further provides an electronic device, the electronic device comprising:

[0079] a memory storing at least one instruction; and

[0080] The processor executes the instructions stored in the memory to implement the above-mentioned method for extracting high-purity konjac glucomannan.

[0081] In order to solve the above problems, the present invention also provides a computer-readable storage medium, wherein at least one instruction is stored in the computer-readable storage medium, and the at least one instruction is executed by a processor in an electronic device to implement the above-mentioned method for extracting high-purity konjac glucomannan.

[0082] The present invention solves the problem described in the background technology. Firstly, a n-hexane solution and a konjac material are obtained, and the n-hexane solution is used to perform a degreasing treatment on the konjac material to obtain a defatted konjac sample, which realizes the removal of grease on the surface of the konjac material and avoids the influence of the grease on the subsequent extraction of high-purity konjac glucomannan. The defatted sample mass of the defatted konjac sample is measured, and a degrading enzyme solution is prepared based on the defatted sample mass. The defatted konjac sample is then subjected to non-sugar degradation by the degrading enzyme solution to obtain an original polysaccharide solution. In this step, the non-konjac glucomannan components in the defatted konjac sample are degraded by the degrading enzyme solution. Since the degradation process of the degradation enzyme does not require the use of a large amount of chemical reagents, the pollution of the subsequent extraction waste liquid to the environment is reduced. At the same time, the degradation enzyme degradation removes the non-konjac glucomannan components, thereby improving the extraction purity of konjac glucomannan. Next, the pH value of the original polysaccharide solution is adjusted to obtain the target polysaccharide solution, which provides good precipitation reaction conditions for the subsequent precipitation of glucomannan. The solution quality of the target polysaccharide solution is measured, and the calcium chloride solution is obtained based on the solution quality, thereby completing the acquisition of the cationic solution, which can precipitate glucomannan, and compared with the traditional ethanol extract, this Cationic impurities in the cationic solution can be filtered in subsequent steps to reduce pollution to the environment. Next, calcium chloride solution is used to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitation solution. The original polysaccharide precipitation solution is subjected to precipitation centrifugation to obtain an original polysaccharide precipitation and an original centrifugal solution. This step successfully separates the glucomannan precipitation and the extracted waste liquid, i.e., the original centrifugal solution. Sodium carbonate solution is used to perform calcium ion reduction operation on the original centrifugal solution to obtain a reduced centrifugal solution. This converts calcium ions into precipitates for subsequent filtration, and then determines whether there is a precipitate in the reduced centrifugal solution. If there is still There is precipitation in the original centrifugal solution, then the reduced centrifugal solution is subjected to precipitation separation to obtain the target centrifugal solution, the target centrifugal solution is used as the target polysaccharide solution, and the step of measuring the solution quality of the target polysaccharide solution is returned, this realizes the repeated glucomannan precipitation operation on the target polysaccharide solution, improves the extraction purity of konjac glucomannan, and simultaneously the calcium ions in the extraction waste liquid are filtered, and the pollution to the environment is reduced, if there is no precipitation in the reduced centrifugal solution, then the original polysaccharide precipitation is collected to obtain the target polysaccharide precipitation, the target polysaccharide precipitation is washed and dried to obtain high-purity konjac glucomannan. Therefore, the present invention can improve the extraction purity of konjac glucomannan and reduce the pollution to the environment of the extraction waste liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0083] Figure 1 A schematic flow diagram of a method for extracting high-purity konjac glucomannan provided by one embodiment of the present invention;

[0084] Figure 2A functional module diagram of a device for extracting high-purity konjac glucomannan provided by one embodiment of the present invention;

[0085] Figure 3 A schematic diagram of the structure of an electronic device for implementing the method for extracting high-purity konjac glucomannan provided in one embodiment of the present invention.

[0086] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0087] It should be understood that the specific embodiments described herein are only used to explain the present invention, and are not used to limit the present invention.

[0088] The embodiment of the present application provides a method for extracting high-purity konjac glucomannan. The execution subject of the method for extracting high-purity konjac glucomannan includes but is not limited to at least one of the electronic devices such as a server and a terminal that can be configured to execute the method provided in the embodiment of the present application. In other words, the method for extracting high-purity konjac glucomannan can be executed by software or hardware installed in a terminal device or a server device, and the software can be a blockchain platform. The server includes but is not limited to: a single server, a server cluster, a cloud server or a cloud server cluster, etc.

[0089] Reference Figure 1 As shown, a schematic flow diagram of an extraction method for high-purity konjac glucomannan provided by one embodiment of the present invention. In this embodiment, the extraction method for high-purity konjac glucomannan comprises:

[0090] S1, obtain n-hexane solution and konjac material, utilize n-hexane solution to defat the konjac material, obtain defatted konjac sample.

[0091] It is understandable that the n-hexane solution refers to a non-polar substance solvent, which can be used to extract and dissolve non-polar substances, wherein the non-polar substance can be: grease. The konjac material refers to the konjac obtained from many aspects, such as: the konjac of agricultural planting and the konjac purchased in the market, wherein the rhizome of the konjac is rich in glucomannan, which is an economical and effective natural food preservative, can effectively prevent food from spoiling, getting moldy and insect pests, and is therefore widely used in fields such as food industry.

[0092] It should be explained that the defatted konjac sample refers to the konjac material after defatting. In the extraction process of glucomannan, n-hexane solution is used to remove the oil components in the konjac material, because these oil components can interfere with the extraction process of glucomannan, wherein the n-hexane solution can also be replaced by other non-polar solvents, for example: petroleum ether solution.

[0093] In detail, the konjac material is defatted by utilizing n-hexane solution to obtain a defatted konjac sample, comprising:

[0094] The konjac material is crushed and dried to obtain a crushed konjac sample;

[0095] Using a pre-built reactor, the n-hexane solution and the crushed konjac sample are mixed and reacted to obtain a mixed konjac sample;

[0096] Based on the preset stirring and dissolving duration, the mixed konjac sample is stirred to obtain a grease separation sample, wherein the grease separation sample is a mixture of a grease mixed solvent and original defatted konjac;

[0097] Described oil separation sample is carried out solvent filtration operation, obtain original defatted konjac, measure original oil content in described original defatted konjac;

[0098] Determining whether the original oil content is less than a preset target oil content;

[0099] If described original oil content is not less than target oil content, then use original defatted konjac as crushing konjac sample, and return described utilization pre-built reactor, described n-hexane solution and crushing konjac sample are carried out the step of mixed reaction;

[0100] If the original oil content is less than the target oil content, the original defatted konjac is dried to obtain a defatted konjac sample.

[0101] Understandably, the crushed konjak sample refers to the konjak material after crushing and drying. The reactor refers to a container for chemical operation, which can be used as a mixed environment in which n-hexane solution is mixed with the crushed konjak sample. The mixed konjak sample refers to the mixture of n-hexane solution and the crushed konjak sample. The stirring and dissolving duration refers to the duration of rapid stirring of the mixed konjak sample, which is set artificially. The grease mixed solvent refers to the mixture of n-hexane and grease, and the original defatted konjak refers to the crushed konjak sample after grease extraction. The original grease content refers to the content of grease in the original defatted konjak, and the target grease content refers to the grease content that needs to be reached, which is set artificially.

[0102] Need to explain, konjac material is pulverized can greatly increase the contact area of ​​konjac material and normal hexane solution, accelerate the separation of grease.Although normal hexane can spontaneously extract grease in the crushed konjac sample in the mixed konjac sample, this spontaneous extraction process is comparatively slow, so can accelerate this process by stirring.When carrying out oil separation in the mixed konjac sample, there will be the phenomenon of oil and solvent separation, wherein, the mixture of oil and solvent is a grease mixed solvent, and the grease mixed solvent can be separated from the grease separation sample, obtain original defatted konjac.When oil content is less than target oil content in original defatted konjac, illustrate that this oil can not extract and have an impact on subsequent glucomannan, wherein, the method for measuring oil content can be: near infrared spectroscopy.

[0103] S2, measuring the defatted sample mass of the defatted konjac sample, preparing a degradative enzyme solution based on the defatted sample mass, utilizing the degradative enzyme solution to carry out non-sugar degradation of the defatted konjac sample to obtain an original polysaccharide solution;

[0104] It is understandable that the defatted sample quality refers to the quality of the defatted konjak sample, and the degradative enzyme solution refers to the degradation solution containing multiple enzymes, which can separate the glucomannan in the defatted konjak sample from the non-glucomannan component by the decomposing power of multiple enzymes, wherein the non-glucomannan comprises: protein, cellulose, pectin and mannan. The original polysaccharide solution refers to the defatted konjak sample after the degradation of the degradative enzyme solution.

[0105] In detail, the preparation of the degradative enzyme solution based on the mass of the defatted sample comprises:

[0106] According to the mass of the defatted sample and the preset unit mass of enzyme activity, a multi-enzyme mass set is calculated, wherein the unit mass of enzyme activity includes: a unit mass of protease, a unit mass of cellulase, a unit mass of pectinase and a unit mass of mannanase, and the multi-enzyme mass set includes: a mass of protease, a mass of cellulase, a mass of pectinase and a mass of mannanase, and the multi-enzyme mass set is expressed as:

[0107] m={m d ,m x ,m v ,m g}

[0108] m d =m t ×u d , m x =m t ×u x , m v =m t ×u v , m g =m t ×ug

[0109] Where m represents the multi-enzyme mass set, m d Indicates the mass of protease, m t Indicates the mass of the defatted sample, u d Indicates the unit mass of protease, m x Indicates the mass of cellulase, u x Indicates the unit mass of cellulase, m v Indicates the quality of pectinase, u v Indicates the unit mass of pectinase, m g Indicates the mass of glycosidase, u g Indicates the unit mass of glycosidase;

[0110] Weighing protease, cellulase, pectinase and mannanase based on the multi-enzyme mass set, and preparing a target buffer based on protease, cellulase, pectinase and mannanase;

[0111] The protease, cellulase, pectinase and mannanase are added to the target buffer to obtain a degradation enzyme solution.

[0112] It can be understood that the protease unit mass refers to the protease mass that needs to be added in every gram of konjac when preparing the target buffer, the cellulase unit mass refers to the cellulase mass that needs to be added in every gram of konjac when preparing the target buffer, the pectinase unit mass refers to the pectinase mass that needs to be added in every gram of konjac when preparing the target buffer, and the mannanase mass that needs to be added in every gram of konjac when preparing the target buffer is referred to by the mannanase unit mass, wherein, the protease can degrade the protein in the konjac, the cellulase can degrade the cellulose in the konjac, the pectinase can degrade the pectin in the konjac, and the mannanase can degrade the mannan in the konjac, for example: β-mannanase.

[0113] It should be explained that since the enzyme has a high substrate specificity, this means that the non-glucomannan components in the defatted konjac sample can be selectively degraded by the degradation enzyme solution without destroying the glucomannan itself. At the same time, compared with the traditional chemical method, the method using the degradation enzyme solution does not require the use of a large amount of chemical reagents, reducing the generation of chemical waste. In addition, the enzyme reaction is usually faster than the chemical reaction time, shortening the preparation time of high-purity konjac glucomannan. Since the conditions of the enzyme reaction are relatively easy to control, such as pH, temperature and time, the entire process of preparing high-purity konjac glucomannan becomes controllable.

[0114] In detail, the target buffer is prepared based on protease, cellulase, pectinase and mannanase, comprising:

[0115] Obtaining the optimal pH ranges of the protease, cellulase, pectinase and mannanase, wherein the optimal pH range is the intersection of the protease pH range, the cellulase pH range, the pectinase pH range and the mannanase pH range;

[0116] Determine a central pH value according to the optimal pH range, wherein the central pH value is the middle value of the optimal pH range;

[0117] According to the central pH value, the preset weak acid concentration and the acid dissociation equilibrium constant, the conjugate base concentration is calculated using the following formula:

[0118]

[0119] Where P represents the central pH value, P k represents the acid dissociation equilibrium constant, lg(*) represents the logarithmic function, C s Indicates the concentration of weak acid, C g represents the conjugate base concentration;

[0120] Prepare a target buffer based on the weak acid concentration and conjugate base concentration.

[0121] It is understandable that the protease pH range, cellulase pH range, pectinase pH range and mannanase pH range refer to the pH activity range of protease, cellulase pH range, pectinase pH range and mannanase pH range, respectively, which can be obtained by searching relevant materials. The central pH value is the middle value of the optimal pH range, for example: if an optimal pH range is {4.5, 4.6, 4.7, 4.8, 4.9}, then the central pH value is 4.7.

[0122] It should be explained that the weak acid concentration refers to the concentration of the weak acid solution, wherein the weak acid solution refers to a solution used to change the pH value of the target buffer solution, such as acetic acid solution. The acid dissociation equilibrium constant refers to a quantitative indicator constant that measures the degree of dissociation of the weak acid solution, which is a fixed value. The conjugate base concentration refers to the concentration of the conjugate base solution, wherein the conjugate base solution refers to a salt solution that reacts with the weak acid solution and can prevent the pH value of the target buffer solution from dropping excessively, such as sodium acetate solution. The target buffer refers to a solvent for mixing multiple enzymes, which has a strong ability to resist changes in pH value, and keeps the pH environment of multiple enzymes from changing too much in subsequent steps.

[0123] In detail, the non-sugar degradation of the defatted konjac sample by a degrading enzyme solution to obtain an original polysaccharide solution comprises:

[0124] The degradative enzyme solution is mixed with the defatted konjac sample to obtain a degraded konjac solution;

[0125] According to the preset degradation time and degradation cycle, the number of acid-base measurements is set, and according to the number of acid-base measurements, the pH of the degraded konjac solution is measured to obtain a real-time degradation solution and a real-time pH value set, wherein the number of acid-base measurements and the real-time pH value set are respectively expressed as:

[0126]

[0127] Where n represents the number of acid-base measurements, t s represents the degradation time, T represents the degradation cycle, P s Represents the real-time pH value set, Indicates the first real-time pH value, Indicates the nth real-time pH value;

[0128] The real-time variance of the real-time pH value set is calculated using the following formula:

[0129]

[0130] Among them, S represents the real-time variance, represents the real-time pH value of the ith Indicates the jth real-time pH value.

[0131] Determining whether the real-time variance is greater than a preset standard variance;

[0132] If the real-time variance is greater than the standard deviation, the real-time degradation solution is used as the degraded konjac solution, and the step of setting the acid-base measurement number according to the preset degradation duration and degradation cycle is returned;

[0133] If the real-time variance is not greater than the standard variance, the real-time degradation solution is subjected to a high temperature enzyme inactivation treatment to obtain an original polysaccharide solution.

[0134] It is understandable that the degraded konjac solution refers to the solution after the degradative enzyme solution is mixed with the defatted konjac sample, and the enzyme degradation activity has not yet begun in the degraded konjac solution, or the enzyme degradation activity is slight. The real-time degradation solution refers to the degraded konjac solution that has carried out the enzyme degradation activity on a large scale. The real-time pH value set refers to the set of the pH value of the real-time degradation solution measured at different times in the degradation duration. The variance between each real-time pH value in the real-time variance value real-time pH value set, the standard deviation refers to the variance of artificial setting, is used to judge whether the non-glucomannan component in the current real-time degradation solution is degraded and completed.

[0135] It should be explained that when the non-glucomannan in the real-time degradation solution is completely degraded, the real-time pH value of the real-time degradation solution will not change significantly in the following time, so the stability of the real-time pH value can be used as an indicator of the completion of the degradation of the non-glucomannan, and the stability of the real-time pH value can be represented by the real-time variance of the real-time pH value set. When the real-time variance is less than the standard variance, it means that the non-glucomannan in the real-time degradation solution is basically degraded. When the degradation is completed, it is necessary to inactivate the various enzymes in the real-time degradation solution to prevent them from affecting subsequent operations, for example: heating the real-time degradation solution to inactivate the various enzymes at high temperature.

[0136] S3. Adjusting the pH of the original polysaccharide solution to obtain a target polysaccharide solution.

[0137] It should be explained that the target polysaccharide solution refers to the original polysaccharide solution with neutral pH. Since the precipitation effect is best when the precipitation environment is neutral in the subsequent precipitation step, the pH of the original polysaccharide solution needs to be neutralized to obtain the target polysaccharide solution.

[0138] In detail, the pH of the original polysaccharide solution is adjusted to obtain the target polysaccharide solution, comprising:

[0139] measuring the original polysaccharide pH value of the original polysaccharide solution;

[0140] Determining whether the original polysaccharide pH value is within a preset polysaccharide pH range;

[0141] If the original polysaccharide pH value is not within the polysaccharide pH range, selecting an acid-base regulator according to the original polysaccharide pH value, and dropping the acid-base regulator into the original polysaccharide solution to obtain an adjusted polysaccharide solution;

[0142] Recording the adjusted polysaccharide solution as the original polysaccharide solution, and returning to the step of measuring the original polysaccharide pH value of the original polysaccharide solution;

[0143] If the original polysaccharide pH value is within the polysaccharide pH range, the original polysaccharide solution is recorded as the target polysaccharide solution.

[0144] It is understandable that the original polysaccharide solution pH value refers to the pH value of the original polysaccharide solution, and the polysaccharide pH range refers to the artificially set pH range, which is a neutral pH range, for example: 6.8 to 7.2. The adjusted polysaccharide solution refers to the original polysaccharide solution after one pH adjustment.

[0145] For example, Xiao Zhang is a staff member of a laboratory. He is extracting high-purity konjac glucomannan. Now Xiao Zhang has obtained the original polysaccharide solution and measured that the pH value of the original polysaccharide solution is 6.2, while the set polysaccharide pH range is 6.8-7.2. Since the solution with a pH value of 6.2 is a slightly acidic solution, Xiao Zhang can choose sodium carbonate as an acid-base regulator to neutralize the acidity in the original polysaccharide solution. Next, Xiao Zhang adds a small amount of sodium carbonate solution to the original polysaccharide solution, and measures the pH value of the original polysaccharide solution again. At this time, it is 6.4, and continues to add a small amount of sodium carbonate solution until the pH value of the original polysaccharide solution is 6.8-7.2.

[0146] S4. Measuring the mass of the target polysaccharide solution, obtaining a calcium chloride solution based on the mass of the solution, and using the calcium chloride solution to perform glucomannan precipitation on the target polysaccharide solution to obtain an original polysaccharide precipitation solution.

[0147] It can be understood that the solution mass refers to the mass of the target polysaccharide solution, the calcium chloride solution can cause the glucomannan in the target polysaccharide solution to form a precipitation, and the original polysaccharide precipitation solution refers to the target polysaccharide solution in which the glucomannan forms a precipitation.

[0148] It should be explained that the calcium ions in the calcium chloride solution can form crosslinks with the carboxyl groups in the glucomannan, thereby precipitating the glucomannan. While using the calcium chloride solution for precipitation, industrial alcohol can be added to further promote the precipitation of the glucomannan. The original polysaccharide precipitation solution obtained after the precipitation of the glucomannan contains a certain amount of calcium ions, so the residual calcium ions need to be filtered later. It should be noted that the solute mass of the calcium chloride solution depends on the mass of the target polysaccharide solution, but the solute mass does not need to be accurately set. It is only necessary to associate the solution mass to obtain the concentration, because multiple additions of calcium chloride solution are required later, and there is a calcium ion filtration step later. For example: if the solution mass of a target polysaccharide solution is 100g, the solute of the calcium chloride solution can be set to 100g.

[0149] In detail, the step of using a calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitated solution comprises:

[0150] The original solute mass of the target polysaccharide solution is measured, and the single addition volume of the calcium chloride solution is calculated based on the original solute mass and the preset precipitation solution concentration, wherein the single addition volume is expressed as:

[0151]

[0152] Where V represents the single addition volume, m y represents the original solute mass, c d represents the concentration of precipitation solution;

[0153] Based on the single precipitation volume, extracting a unit precipitation reagent from the calcium chloride solution, and adding the unit precipitation reagent to the target polysaccharide solution to obtain a precipitation reaction solution;

[0154] Based on the preset alcohol volume, pre-obtained industrial alcohol is added to the precipitation reaction solution to obtain an original polysaccharide precipitation solution.

[0155] It can be understood that the original solute mass refers to the mass of the solute in the target polysaccharide solution, the precipitation solution concentration refers to the concentration of the calcium chloride solution, the single addition volume refers to the volume of the calcium chloride solution added to the target polysaccharide solution at a single time, and the unit precipitation reagent refers to the calcium chloride solution with a volume of a single addition volume. The precipitation reaction reagent refers to the target polysaccharide solution after adding the unit precipitation reagent, and the industrial alcohol has a high concentration of ethanol. The alcohol volume is an artificially set volume, and adding a small amount of industrial alcohol can further increase the precipitation rate of glucomannan.

[0156] It should be explained that the purpose of single addition is to prevent too much calcium chloride from being added at one time, which makes the subsequent filtration part too complicated. Therefore, it is not necessary to be too precise when calculating the volume of a single addition, because multiple additions will gradually precipitate the glucomannan in the target polysaccharide solution, which reduces the difficulty of calculation during the extraction process and improves the efficiency of the extraction. At the same time, after adding a unit precipitation reagent to obtain a precipitation reaction solution, it is necessary to add industrial alcohol, which is to further accelerate the precipitation rate of glucomannan, because ethanol will reduce the solubility of glucomannan, but because industrial alcohol has the defects of being difficult to filter, high cost and dangerous to prepare, in this precipitation step, industrial alcohol is only added in a very small amount as a reagent to promote precipitation, so the volume of industrial alcohol needs to be controlled.

[0157] S5. The original polysaccharide precipitation solution is subjected to precipitation centrifugal separation to obtain original polysaccharide precipitation and original centrifugal solution, and the original centrifugal solution is subjected to calcium ion reduction operation using a preset sodium carbonate solution to obtain a reduced centrifugal solution.

[0158] It can be understood that the original polysaccharide precipitate refers to the precipitated material in the original polysaccharide precipitation solution, and the original polysaccharide precipitate should be glucomannan precipitate. The original centrifuged solution refers to the non-precipitated part in the original polysaccharide precipitation solution, and the original centrifuged solution may contain calcium ions. The sodium carbonate solution refers to a solution used to filter calcium ions. The reduced centrifuged solution refers to the original centrifuged solution after the calcium ion reduction operation, and the reduced centrifuged solution may contain calcium carbonate precipitate.

[0159] It should be explained that since sodium carbonate reacts chemically with calcium ions to produce calcium carbonate precipitation, the calcium ions in the reduced centrifugal solution can be filtered.

[0160] In detail, the original polysaccharide precipitation solution is subjected to precipitation centrifugation to obtain the original polysaccharide precipitation and the original centrifugation solution, comprising:

[0161] Transferring the original glycan precipitate to a pre-constructed centrifuge tube cluster to obtain a plurality of original solutions to be separated, wherein the centrifuge tube cluster is placed on a plurality of centrifuge radius circles of a centrifuge rotor;

[0162] Performing a solution balancing operation on the multiple original solutions to be separated to obtain multiple target solutions to be separated, wherein the solution balancing operation refers to balancing the masses of the multiple original solutions to be separated;

[0163] Measuring the distances between the bottoms of the plurality of centrifuge tubes and the center of the centrifuge rotor to obtain a plurality of centrifugal distances, and calculating an average centrifugal distance of the plurality of centrifugal distances;

[0164] The rotor speed is determined according to the average centrifugal distance and the preset relative centrifugal force, wherein the rotor speed is expressed as:

[0165]

[0166] Among them, r represents the rotor speed, R represents the relative centrifugal force, and l represents the average centrifugal distance;

[0167] According to the rotor speed and the preset separation time, the plurality of target solutions to be separated are centrifuged to obtain a plurality of target separation precipitates and a plurality of target separation solutions;

[0168] The multiple target separation precipitates and the multiple target separation solutions are respectively combined to obtain an original glycan precipitate and an original centrifugation solution.

[0169] It is understood that the centrifuge tube cluster refers to a collection of containers for placing the original solution to be separated, wherein the centrifuge tube is part of a centrifuge, and the centrifuge further includes: a centrifuge rotor, wherein the centrifuge rotor is connected to the centrifuge tube cluster, the number of centrifuge tubes contained in the centrifuge tube cluster depends on the centrifuge, and the solution in the centrifuge tube is driven to precipitate and separate by the rotation of the centrifuge rotor, and the centrifuge radius circle is a circumference determined according to the radius of the centrifuge rotor, and the centrifuge radius circle is a circumference set by an artificial person. The target solution to be separated refers to the original solution to be separated after the balancing operation, the centrifugal distance refers to the distance between the bottom of the centrifuge tube and the centrifugal rotor, and the average centrifugal distance refers to the average value of multiple centrifugal distances.

[0170] It can be understood that the relative centrifugal force refers to the force acting on the target solution to be separated due to the rotation of the centrifuge rotor during the centrifugation process. The relative centrifugal force can be set manually, and some laboratories have standard fixed values ​​for the relative centrifugal force. The rotor speed refers to the rotation speed of the centrifuge rotor during centrifugal work. The separation time refers to the time for the separation operation using the centrifuge, which is set manually. The target separation precipitate refers to the precipitate separated from the target solution to be separated. The target separation solution refers to the solution separated from the target solution to be separated after centrifugal separation.

[0171] It should be explained that since the relative centrifugal value is a manually set value, it is necessary to calculate the rotor speed and input the rotor speed into the control panel of the centrifuge. Since the radius difference of multiple centrifugal radius circles is very small, the average centrifugal distance can be used in the calculation of the rotor speed, which can greatly simplify the actual operation time and reduce the complexity of the operation. In addition, setting the rotor speed of the centrifuge is simpler than directly setting the relative centrifugal value, which also reduces the technical requirements for the operator.

[0172] S6. Determine whether there is precipitation in the reduced centrifugal solution.

[0173] It should be explained that by judging whether there is a precipitate in the reduced centrifuged solution, it can be judged whether the glucomannan is completely precipitated. If there is no precipitate in the reduced centrifuged solution, it means that all calcium ions are involved in the precipitation reaction of glucomannan, so it means that there may be glucomannan in the reduced centrifuged solution that has not been precipitated, because glucomannan is very unlikely to just react completely with calcium ions. If there is no precipitate in the reduced centrifuged solution, it means that the calcium ions have not completely reacted with the glucomannan for precipitation, which means that the reduced centrifuged solution no longer contains glucomannan, indicating that the precipitation is complete.

[0174] If there is precipitation in the reduced centrifugal solution, execute S7, perform precipitation separation on the reduced centrifugal solution to obtain a target centrifugal solution, use the target centrifugal solution as a target glycan solution, and return to the step of measuring the solution quality of the target glycan solution.

[0175] It can be understood that the target centrifugal solution refers to the reduced centrifugal solution after precipitation separation. Since there is no precipitation in the reduced centrifugal solution, it means that the reduced centrifugal solution does not contain calcium ions or the calcium ion content is extremely small, and thus the reduced centrifugal solution also has minimal damage to the environment.

[0176] If there is no precipitate in the reduced centrifugal solution, S8 is executed to collect the original polysaccharide precipitates to obtain target polysaccharide precipitates, and the target polysaccharide precipitates are washed and dried to obtain high-purity konjac glucomannan.

[0177] In detail, the target polysaccharide precipitate is washed and dried to obtain high-purity konjac glucomannan, comprising:

[0178] Measuring a target precipitation volume of the target glycan precipitation, and obtaining high-concentration ethanol based on the target precipitation volume, wherein the ethanol concentration of the high-concentration ethanol is greater than 95%;

[0179] adding the high concentration ethanol to the target polysaccharide precipitate to obtain a washed polysaccharide precipitate, and contacting the washed polysaccharide precipitate with ethanol to obtain a precipitate ethanol mixture;

[0180] According to a preset static time, the impurities of the precipitation ethanol mixture are dissolved to obtain a dissolved mixture, and the dissolved mixture is centrifuged to obtain a precipitation sedimentation mixture, wherein the precipitation sedimentation mixture comprises: an ethanol clear solution and a sedimentation substrate;

[0181] The ethanol clear liquid in the precipitation and sedimentation mixture is removed to obtain a sedimentation substrate, and the sedimentation substrate is washed and dried with the pre-obtained anhydrous ethanol to obtain high-purity konjac glucomannan.

[0182] It can be understood that the target precipitation volume refers to the volume of the target polysaccharide precipitation, and the washed polysaccharide precipitation refers to a mixture of the target polysaccharide precipitation and high-concentration ethanol. The precipitation ethanol mixture refers to a mixture after the target polysaccharide precipitation is fully contacted with the high-concentration ethanol, wherein the washing polysaccharide precipitation can be fully contacted by gently shaking the washed polysaccharide precipitation. The dissolved mixture refers to the precipitation ethanol mixture after the impurities are dissolved, wherein the high-concentration ethanol will dissolve the impurities in the target polysaccharide precipitation. The upper layer of the precipitation sedimentation mixture is an ethanol clear solution, and the lower layer is a sedimentation substrate, wherein the ethanol clear solution refers to a mixed solution containing high-concentration ethanol and impurities, and the sedimentation substrate is the target polysaccharide precipitation after the impurities are dissolved.

[0183] It should be explained that after washing the target polysaccharide precipitate with high-concentration ethanol, a precipitate ethanol mixture is obtained, but there are still a small amount of impurities in the precipitate ethanol mixture, such as water in the high-concentration ethanol, a small amount of precipitated cations, etc. Since the cost of using anhydrous ethanol is relatively high, when washing the precipitate, the target polysaccharide precipitate can be initially washed with relatively low-cost high-concentration ethanol to remove most of the impurities, and finally washed with a small amount of anhydrous ethanol.

[0184] The present invention solves the problem described in the background technology. The present invention first obtains a n-hexane solution and a konjac material, and utilizes the n-hexane solution to perform a defatting treatment on the konjac material to obtain a defatted konjac sample, which realizes the removal of grease on the surface of the konjac material, and avoids the influence of grease on the subsequent extraction of high-purity konjac glucomannan, and measures the defatted sample mass of the defatted konjac sample, and prepares a degrading enzyme solution based on the defatted sample mass, and utilizes the degrading enzyme solution to perform non-sugar degradation on the defatted konjac sample to obtain an original polysaccharide solution, and this step realizes the degradation of non-konjac glucomannan components in the defatted konjac sample by the degrading enzyme solution. Degradation, since the degradation process of the degradation enzyme does not require the use of a large amount of chemical reagents, the pollution of the subsequent extraction waste liquid to the environment is reduced. At the same time, the degradation enzyme degradation removes the non-konjac glucomannan components, thereby improving the extraction purity of the konjac glucomannan. Next, the pH value of the original polysaccharide solution is adjusted to obtain the target polysaccharide solution, which provides good precipitation reaction conditions for the subsequent precipitation of glucomannan. The solution quality of the target polysaccharide solution is measured, and the calcium chloride solution is obtained based on the solution quality, thereby completing the acquisition of the cationic solution, which can precipitate glucomannan, and compared with the traditional ethanol extract, The cationic impurities in the cationic solution can be filtered in the subsequent steps to reduce pollution to the environment. Next, the target polysaccharide solution is subjected to glucomannan precipitation using a calcium chloride solution to obtain an original polysaccharide precipitation solution. The original polysaccharide precipitation solution is subjected to precipitation centrifugation to obtain an original polysaccharide precipitation and an original centrifugal solution. This step successfully separates the glucomannan precipitation and the extracted waste liquid, i.e., the original centrifugal solution. The original centrifugal solution is subjected to a calcium ion reduction operation using a sodium carbonate solution to obtain a reduced centrifugal solution. This converts the calcium ions into precipitates for subsequent filtration. Then, it is determined whether there is a precipitate in the reduced centrifugal solution. If there is still There is precipitation in the original centrifugal solution, then the reduced centrifugal solution is subjected to precipitation separation to obtain the target centrifugal solution, the target centrifugal solution is used as the target polysaccharide solution, and the step of measuring the solution quality of the target polysaccharide solution is returned, this realizes the repeated glucomannan precipitation operation on the target polysaccharide solution, improves the extraction purity of konjac glucomannan, and simultaneously the calcium ions in the extraction waste liquid are filtered, and the pollution to the environment is reduced, if there is no precipitation in the reduced centrifugal solution, then the original polysaccharide precipitation is collected to obtain the target polysaccharide precipitation, the target polysaccharide precipitation is washed and dried to obtain high-purity konjac glucomannan. Therefore, the present invention can improve the extraction purity of konjac glucomannan and reduce the pollution to the environment of the extraction waste liquid.

[0185] like Figure 2 , which is a functional module diagram of a device for extracting high-purity konjac glucomannan provided by one embodiment of the present invention.

[0186] The extraction device 100 for high-purity konjac glucomannan of the present invention can be installed in an electronic device. According to the functions to be implemented, the extraction device 100 for high-purity konjac glucomannan can include a raw polysaccharide collection module 101, a polysaccharide precipitation generation module 102, a reduction solution acquisition module 103 and a glucomannan preparation module 104. The module of the present invention can also be called a unit, which refers to a series of computer program segments that can be executed by an electronic device processor and can complete fixed functions, which are stored in the memory of the electronic device.

[0187] The original polysaccharide collection module 101 is used to obtain a n-hexane solution and a konjac material, perform a defatting treatment on the konjac material using the n-hexane solution to obtain a defatted konjac sample, measure the defatted sample mass of the defatted konjac sample, prepare a degradative enzyme solution based on the defatted sample mass, and perform non-sugar degradation on the defatted konjac sample using the degradative enzyme solution to obtain an original polysaccharide solution;

[0188] The polysaccharide precipitation generating module 102 is used to adjust the pH of the original polysaccharide solution to obtain a target polysaccharide solution, measure the solution quality of the target polysaccharide solution, obtain a calcium chloride solution based on the solution quality, and use the calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitation solution;

[0189] The reducing solution acquisition module 103 is used to perform precipitation centrifugation separation on the original polysaccharide precipitation solution to obtain the original polysaccharide precipitation and the original centrifugal solution, and perform calcium ion reduction operation on the original centrifugal solution using a preset sodium carbonate solution to obtain a reducing centrifugal solution;

[0190] The glucomannan preparation module 104 is used to determine whether there is a precipitate in the reduced centrifugal solution. If there is a precipitate in the reduced centrifugal solution, the reduced centrifugal solution is subjected to precipitation separation to obtain a target centrifugal solution, the target centrifugal solution is used as a target polysaccharide solution, and the step of measuring the solution quality of the target polysaccharide solution is returned. If there is no precipitate in the reduced centrifugal solution, the original polysaccharide precipitate is collected to obtain a target polysaccharide precipitate, and the target polysaccharide precipitate is washed and dried to obtain high-purity konjac glucomannan.

[0191] In detail, the modules in the extraction device 100 for high-purity konjac glucomannan described in the embodiment of the present invention are used in the same manner as described above. Figure 1 The same technical means as described in the method for extracting high-purity konjac glucomannan can produce the same technical effects, which will not be repeated here.

[0192] like Figure 3, which is a schematic diagram of the structure of an electronic device for implementing a method for extracting high-purity konjac glucomannan provided by one embodiment of the present invention.

[0193] The electronic device 1 may include a processor 10, a memory 11 and a bus 12, and may also include a computer program stored in the memory 11 and executable on the processor 10, such as a program for extracting high-purity konjac glucomannan.

[0194] Wherein, the memory 11 includes at least one type of readable storage medium, and the readable storage medium includes flash memory, mobile hard disk, multimedia card, card-type memory (for example: SD or DX memory, etc.), magnetic memory, disk, optical disk, etc. The memory 11 may be an internal storage unit of the electronic device 1 in some embodiments, such as a mobile hard disk of the electronic device 1. The memory 11 may also be an external storage device of the electronic device 1 in other embodiments, such as a plug-in mobile hard disk, a smart memory card (Smart Media Card, SMC), a secure digital (SecureDigital, SD) card, a flash card (Flash Card), etc. equipped on the electronic device 1. Further, the memory 11 also includes an internal storage unit of the electronic device 1 and an external storage device. The memory 11 can not only be used to store application software and various types of data installed in the electronic device 1, such as the code of the extraction method program for high-purity konjac glucomannan, but also can be used to temporarily store data that has been output or is to be output.

[0195] In some embodiments, the processor 10 may be composed of an integrated circuit, for example, a single packaged integrated circuit, or a plurality of packaged integrated circuits with the same or different functions, including one or more central processing units (CPUs), microprocessors, digital processing chips, graphics processors, and combinations of various control chips, etc. The processor 10 is the control core (Control Unit) of the electronic device, and uses various interfaces and lines to connect various components of the entire electronic device, and executes or executes programs or modules stored in the memory 11 (such as a program for extracting high-purity konjac glucomannan, etc.), and calls data stored in the memory 11 to execute various functions of the electronic device 1 and process data.

[0196] The bus 12 may be a peripheral component interconnect (PCI) bus or an extended industry standard architecture (EISA) bus, etc. The bus 12 may be divided into an address bus, a data bus, a control bus, etc. The bus 12 is configured to realize connection and communication between the memory 11 and at least one processor 10, etc.

[0197] Figure 3 Only an electronic device with components is shown, and those skilled in the art will understand that Figure 3 The structure shown does not constitute a limitation on the electronic device 1, and may include fewer or more components than shown in the figure, or combine certain components, or arrange the components differently.

[0198] For example, although not shown, the electronic device 1 may also include a power source (such as a battery) for supplying power to each component. Preferably, the power source may be logically connected to the at least one processor 10 through a power management device, so that the power management device can realize functions such as charging management, discharging management, and power consumption management. The power source may also include any components such as one or more DC or AC power sources, recharging devices, power failure detection circuits, power converters or inverters, power status indicators, etc. The electronic device 1 may also include a variety of sensors, Bluetooth modules, Wi-Fi modules, etc., which will not be repeated here.

[0199] Furthermore, the electronic device 1 may also include a network interface. Optionally, the network interface may include a wired interface and / or a wireless interface (such as a WI-FI interface, a Bluetooth interface, etc.), which is generally used to establish a communication connection between the electronic device 1 and other electronic devices.

[0200] Optionally, the electronic device 1 may further include a user interface, which may be a display, an input unit (such as a keyboard), or a standard wired interface or a wireless interface. Optionally, in some embodiments, the display may be an LED display, a liquid crystal display, a touch-sensitive liquid crystal display, and an OLED (Organic Light-Emitting Diode) touch device. The display may also be appropriately referred to as a display screen or a display unit, which is used to display information processed in the electronic device 1 and to display a visual user interface.

[0201] The program for extracting high-purity konjac glucomannan stored in the memory 11 of the electronic device 1 is a combination of multiple instructions. When running in the processor 10, the following can be achieved:

[0202] Obtaining a n-hexane solution and a konjac material, and utilizing the n-hexane solution to defat the konjac material to obtain a defatted konjac sample;

[0203] Measuring the defatted sample mass of the defatted konjac sample, preparing a degradation enzyme solution based on the defatted sample mass, and performing non-sugar degradation on the defatted konjac sample using the degradation enzyme solution to obtain an original polysaccharide solution;

[0204] adjusting the pH of the original polysaccharide solution to obtain a target polysaccharide solution;

[0205] measuring the mass of the target polysaccharide solution, obtaining a calcium chloride solution based on the mass of the solution, and using the calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitated solution;

[0206] The original polysaccharide precipitation solution is subjected to precipitation centrifugation separation to obtain an original polysaccharide precipitation and an original centrifugal solution, and the original centrifugal solution is subjected to a calcium ion reduction operation using a preset sodium carbonate solution to obtain a reduced centrifugal solution;

[0207] Determining whether there is precipitation in the reduced centrifugal solution;

[0208] If there is precipitation in the reduced centrifugal solution, the reduced centrifugal solution is subjected to precipitation separation to obtain a target centrifugal solution, the target centrifugal solution is used as a target glycan solution, and the process returns to the step of measuring the solution quality of the target glycan solution;

[0209] If there is no precipitate in the reduced centrifugal solution, the original polysaccharide precipitates are collected to obtain target polysaccharide precipitates, and the target polysaccharide precipitates are washed and dried to obtain high-purity konjac glucomannan.

[0210] Specifically, the specific implementation method of the processor 10 for the above instructions can refer to Figures 1 to 3 The description of the relevant steps in the corresponding embodiments will not be repeated here.

[0211] Furthermore, if the module / unit integrated in the electronic device 1 is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. The computer-readable storage medium can be volatile or non-volatile. For example, the computer-readable medium can include: any entity or device capable of carrying the computer program code, a recording medium, a USB flash drive, a mobile hard disk, a magnetic disk, an optical disk, a computer memory, and a read-only memory (ROM).

[0212] The present invention further provides a computer-readable storage medium, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor of an electronic device, the computer program can implement:

[0213] Obtaining a n-hexane solution and a konjac material, and utilizing the n-hexane solution to defat the konjac material to obtain a defatted konjac sample;

[0214] Measuring the defatted sample mass of the defatted konjac sample, preparing a degradation enzyme solution based on the defatted sample mass, and performing non-sugar degradation on the defatted konjac sample using the degradation enzyme solution to obtain an original polysaccharide solution;

[0215] adjusting the pH of the original polysaccharide solution to obtain a target polysaccharide solution;

[0216] measuring the mass of the target polysaccharide solution, obtaining a calcium chloride solution based on the mass of the solution, and using the calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitated solution;

[0217] The original polysaccharide precipitation solution is subjected to precipitation centrifugation separation to obtain an original polysaccharide precipitation and an original centrifugal solution, and the original centrifugal solution is subjected to a calcium ion reduction operation using a preset sodium carbonate solution to obtain a reduced centrifugal solution;

[0218] Determining whether there is precipitation in the reduced centrifugal solution;

[0219] If there is precipitation in the reduced centrifugal solution, the reduced centrifugal solution is subjected to precipitation separation to obtain a target centrifugal solution, the target centrifugal solution is used as a target glycan solution, and the process returns to the step of measuring the solution quality of the target glycan solution;

[0220] If there is no precipitate in the reduced centrifugal solution, the original polysaccharide precipitates are collected to obtain target polysaccharide precipitates, and the target polysaccharide precipitates are washed and dried to obtain high-purity konjac glucomannan.

[0221] In the several embodiments provided by the present invention, it should be understood that the disclosed devices, apparatuses and methods can be implemented in other ways. For example, the device embodiments described above are only illustrative, and actual implementation may have other division methods.

[0222] The modules described as separate components may or may not be physically separated, and the components shown as modules may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0223] In addition, each functional module in each embodiment of the present invention may be integrated into one processing unit, each unit may exist physically separately, or two or more units may be integrated into one unit. The above-mentioned integrated unit may be implemented in the form of hardware or in the form of hardware plus software functional modules.

[0224] It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention.

[0225] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A method for extracting high-purity konjac glucomannan, characterized in that: The method comprises: Obtaining a n-hexane solution and a konjac material, and utilizing the n-hexane solution to defat the konjac material to obtain a defatted konjac sample; Measuring the defatted sample mass of the defatted konjac sample, preparing a degradation enzyme solution based on the defatted sample mass, and performing non-sugar degradation on the defatted konjac sample using the degradation enzyme solution to obtain an original polysaccharide solution; adjusting the pH of the original polysaccharide solution to obtain a target polysaccharide solution; measuring the mass of the target polysaccharide solution, obtaining a calcium chloride solution based on the mass of the solution, and using the calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitated solution; The original polysaccharide precipitation solution is subjected to precipitation centrifugation separation to obtain an original polysaccharide precipitation and an original centrifugal solution, and the original centrifugal solution is subjected to a calcium ion reduction operation using a preset sodium carbonate solution to obtain a reduced centrifugal solution; Determining whether there is precipitation in the reduced centrifugal solution; If there is precipitation in the reduced centrifugal solution, the reduced centrifugal solution is subjected to precipitation separation to obtain a target centrifugal solution, the target centrifugal solution is used as a target glycan solution, and the process returns to the step of measuring the solution quality of the target glycan solution; If there is no precipitate in the reduced centrifugal solution, the original polysaccharide precipitates are collected to obtain target polysaccharide precipitates, and the target polysaccharide precipitates are washed and dried to obtain high-purity konjac glucomannan.

2. The method for extracting high-purity konjac glucomannan as claimed in claim 1, wherein: The method comprises the following steps: degreasing the konjac material using n-hexane solution to obtain a defatted konjac sample; and The konjac material is crushed and dried to obtain a crushed konjac sample; Using a pre-built reactor, the n-hexane solution and the crushed konjac sample are mixed and reacted to obtain a mixed konjac sample; Based on the preset stirring and dissolving duration, the mixed konjac sample is stirred to obtain a grease separation sample, wherein the grease separation sample is a mixture of a grease mixed solvent and original defatted konjac; Described oil separation sample is carried out solvent filtration operation, obtain original defatted konjac, measure original oil content in described original defatted konjac; Determining whether the original oil content is less than a preset target oil content; If described original oil content is not less than target oil content, then use original defatted konjac as crushing konjac sample, and return described utilization pre-built reactor, described n-hexane solution and crushing konjac sample are carried out the step of mixed reaction; If the original oil content is less than the target oil content, the original defatted konjac is dried to obtain a defatted konjac sample.

3. The method for extracting high-purity konjac glucomannan as claimed in claim 2, wherein: The method of preparing a degradative enzyme solution based on the mass of the defatted sample comprises: According to the mass of the defatted sample and the preset unit mass of enzyme activity, a multi-enzyme mass set is calculated, wherein the unit mass of enzyme activity includes: a unit mass of protease, a unit mass of cellulase, a unit mass of pectinase and a unit mass of mannanase, and the multi-enzyme mass set includes: a mass of protease, a mass of cellulase, a mass of pectinase and a mass of mannanase, and the multi-enzyme mass set is expressed as: m={m d ,m x ,m v ,m g } m d =m t ×u d ,m x =m t ×u x ,m v =m t ×u v ,m g =m t ×u g Where m represents the multi-enzyme mass set, m d Indicates the mass of protease, m t Indicates the mass of the defatted sample, u d Indicates the unit mass of protease, m x Indicates the mass of cellulase, u x Indicates the unit mass of cellulase, m v Indicates the quality of pectinase, u v Indicates the unit mass of pectinase, m g Indicates the mass of glycosidase, u g Indicates the unit mass of glycosidase; Weighing protease, cellulase, pectinase and mannanase based on the multi-enzyme mass set, and preparing a target buffer based on protease, cellulase, pectinase and mannanase; The protease, cellulase, pectinase and mannanase are added to the target buffer to obtain a degradation enzyme solution.

4. The method for extracting high-purity konjac glucomannan as claimed in claim 3, characterized in that: The method of preparing a target buffer based on protease, cellulase, pectinase and mannanase comprises: Obtaining the optimal pH ranges of the protease, cellulase, pectinase and mannanase, wherein the optimal pH range is the intersection of the protease pH range, the cellulase pH range, the pectinase pH range and the mannanase pH range; Determine a central pH value according to the optimal pH range, wherein the central pH value is the middle value of the optimal pH range; According to the central pH value, the preset weak acid concentration and the acid dissociation equilibrium constant, the conjugate base concentration is calculated using the following formula: Where P represents the central pH value, P k represents the acid dissociation equilibrium constant, lg(*) represents the logarithmic function, C s Indicates the concentration of weak acid, C g represents the conjugate base concentration; Prepare a target buffer based on the weak acid concentration and conjugate base concentration.

5. The method for extracting high-purity konjac glucomannan as claimed in claim 4, characterized in that: The method of using a degradative enzyme solution to perform non-sugar degradation on the defatted konjac sample to obtain an original polysaccharide solution comprises: The degradative enzyme solution is mixed with the defatted konjac sample to obtain a degraded konjac solution; According to the preset degradation time and degradation cycle, the number of acid-base measurements is set, and according to the number of acid-base measurements, the pH of the degraded konjac solution is measured to obtain a real-time degradation solution and a real-time pH value set, wherein the number of acid-base measurements and the real-time pH value set are respectively expressed as: Where n represents the number of acid-base measurements, t s represents the degradation time, T represents the degradation cycle, P s Represents the real-time pH value set, Indicates the first real-time pH value, Indicates the nth real-time pH value; The real-time variance of the real-time pH value set is calculated using the following formula: Among them, S represents the real-time variance, represents the real-time pH value of the ith Indicates the jth real-time pH value. Determining whether the real-time variance is greater than a preset standard variance; If the real-time variance is greater than the standard deviation, the real-time degradation solution is used as the degraded konjac solution, and the step of setting the acid-base measurement number according to the preset degradation duration and degradation cycle is returned; If the real-time variance is not greater than the standard variance, the real-time degradation solution is subjected to a high temperature enzyme inactivation treatment to obtain an original polysaccharide solution.

6. The method for extracting high-purity konjac glucomannan as claimed in claim 5, characterized in that: The step of adjusting the pH of the original polysaccharide solution to obtain the target polysaccharide solution comprises: measuring the original polysaccharide pH value of the original polysaccharide solution; Determining whether the original polysaccharide pH value is within a preset polysaccharide pH range; If the original polysaccharide pH value is not within the polysaccharide pH range, selecting an acid-base regulator according to the original polysaccharide pH value, and dropping the acid-base regulator into the original polysaccharide solution to obtain an adjusted polysaccharide solution; Recording the adjusted polysaccharide solution as the original polysaccharide solution, and returning to the step of measuring the original polysaccharide pH value of the original polysaccharide solution; If the original polysaccharide pH value is within the polysaccharide pH range, the original polysaccharide solution is recorded as the target polysaccharide solution.

7. The method for extracting high-purity konjac glucomannan as claimed in claim 6, characterized in that: The method of using a calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitation solution comprises: The original solute mass of the target polysaccharide solution is measured, and the single addition volume of the calcium chloride solution is calculated based on the original solute mass and the preset precipitation solution concentration, wherein the single addition volume is expressed as: Where V represents the single addition volume, m y represents the original solute mass, c d represents the concentration of precipitation solution; Based on the single precipitation volume, extracting a unit precipitation reagent from the calcium chloride solution, and adding the unit precipitation reagent to the target polysaccharide solution to obtain a precipitation reaction solution; Based on the preset alcohol volume, the pre-obtained industrial alcohol is added to the precipitation reaction solution to obtain the original polysaccharide precipitation solution.

8. The method for extracting high-purity konjac glucomannan as claimed in claim 7, characterized in that: The method of subjecting the original polysaccharide precipitation solution to precipitation centrifugation to obtain the original polysaccharide precipitation and the original centrifugation solution comprises: Transferring the original glycan precipitate to a pre-constructed centrifuge tube cluster to obtain a plurality of original solutions to be separated, wherein the centrifuge tube cluster is placed on a plurality of centrifuge radius circles of a centrifuge rotor; Performing a solution balancing operation on the multiple original solutions to be separated to obtain multiple target solutions to be separated, wherein the solution balancing operation refers to balancing the masses of the multiple original solutions to be separated; Measuring the distances between the bottoms of the plurality of centrifuge tubes and the center of the centrifuge rotor to obtain a plurality of centrifugal distances, and calculating an average centrifugal distance of the plurality of centrifugal distances; The rotor speed is determined according to the average centrifugal distance and the preset relative centrifugal force, wherein the rotor speed is expressed as: Among them, r represents the rotor speed, R represents the relative centrifugal force, and l represents the average centrifugal distance; According to the rotor speed and the preset separation time, the plurality of target solutions to be separated are centrifuged to obtain a plurality of target separation precipitates and a plurality of target separation solutions; The multiple target separation precipitates and the multiple target separation solutions are respectively combined to obtain an original glycan precipitate and an original centrifugation solution.

9. The method for extracting high-purity konjac glucomannan as claimed in claim 8, characterized in that: The target polysaccharide precipitate is washed and dried to obtain high-purity konjac glucomannan, comprising: Measuring a target precipitation volume of the target glycan precipitation, and obtaining high-concentration ethanol based on the target precipitation volume, wherein the ethanol concentration of the high-concentration ethanol is greater than 95%; adding the high concentration ethanol to the target polysaccharide precipitate to obtain a washed polysaccharide precipitate, and contacting the washed polysaccharide precipitate with ethanol to obtain a precipitate ethanol mixture; According to a preset static time, the impurities of the precipitation ethanol mixture are dissolved to obtain a dissolved mixture, and the dissolved mixture is centrifuged to obtain a precipitation sedimentation mixture, wherein the precipitation sedimentation mixture comprises: an ethanol clear solution and a sedimentation substrate; The ethanol clear liquid in the precipitation and sedimentation mixture is removed to obtain a sedimentation substrate, and the sedimentation substrate is washed and dried with the pre-obtained anhydrous ethanol to obtain high-purity konjac glucomannan.

10. An extraction device for high-purity konjac glucomannan, characterized in that: The device comprises: The original polysaccharide collection module is used to obtain a n-hexane solution and a konjac material, perform a defatting treatment on the konjac material using the n-hexane solution to obtain a defatted konjac sample, measure the defatted sample mass of the defatted konjac sample, prepare a degradative enzyme solution based on the defatted sample mass, and perform non-sugar degradation on the defatted konjac sample using the degradative enzyme solution to obtain an original polysaccharide solution; a polysaccharide precipitation generation module, which is used to adjust the pH of the original polysaccharide solution to obtain a target polysaccharide solution, measure the solution mass of the target polysaccharide solution, obtain a calcium chloride solution based on the solution mass, and use the calcium chloride solution to precipitate glucomannan on the target polysaccharide solution to obtain an original polysaccharide precipitation solution; A reduction solution acquisition module is used to perform precipitation centrifugation separation on the original polysaccharide precipitation solution to obtain original polysaccharide precipitation and original centrifugal solution, and perform calcium ion reduction operation on the original centrifugal solution using a preset sodium carbonate solution to obtain a reduction centrifugal solution; The glucomannan preparation module is used to determine whether there is precipitation in the reduced centrifugal solution. If there is precipitation in the reduced centrifugal solution, the reduced centrifugal solution is subjected to precipitation separation to obtain a target centrifugal solution, the target centrifugal solution is used as a target polysaccharide solution, and the step of measuring the solution quality of the target polysaccharide solution is returned. If there is no precipitation in the reduced centrifugal solution, the original polysaccharide precipitation is collected to obtain a target polysaccharide precipitation, and the target polysaccharide precipitation is washed and dried to obtain high-purity konjac glucomannan.

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