Gynecological probiotic freeze-dried active bacterium bacteriostatic tablet and preparation method thereof

Abstract

The invention discloses a probiotic freeze-dried active bacterium bacteriostatic tablet for gynecology. The probiotic freeze-dried active bacterium bacteriostatic tablet is specifically prepared from the following components in parts by weight: active bacteria of probiotics, a freeze-drying protective agent, soybean peptide powder, montmorillonite, D-mannitol, fructo-oligosaccharide, sorbitol, microcrystalline cellulose, chlorhexidine acetate and magnesium stearate. The invention further discloses a preparation method of the gynecological probiotic freeze-dried active bacterium bacteriostatic tablet. According to the invention, the survival rate and the survival time of probiotics are effectively prolonged, and the purpose of micro-ecological balance is improved, so that the effect of preventing original bacteria from breeding is achieved.

Description

Technical Field

[0001] The invention relates to a gynecological probiotic freeze-dried active bacteria antibacterial tablet and a preparation method thereof. Background Art

[0002] Gynecological diseases are common and frequently occurring diseases among women. Many people lack proper knowledge of gynecological diseases, lack of health care, and various bad living habits, which gradually reduce their physical health. This leads to some women being plagued by diseases that cannot be cured for a long time, causing great inconvenience to their normal life and work. Traditional oral medications cannot be fully absorbed and are excreted due to the special location of the disease.

[0003] At present, the commonly used antibacterial and antifungal drugs for the treatment of gynecological diseases are all to control inflammation by killing or inhibiting pathogens. If they cannot be completely killed at one time, they will soon come back. On the one hand, bacteria and fungi can develop drug resistance; on the other hand, antibiotics will kill beneficial bacteria while killing vaginal pathogens, such as lactobacilli that play a role in vaginal self-cleaning. This will cause vaginal flora disorders while treating inflammation, making it more likely to cause repeated attacks of gynecological inflammation. Recent studies have shown that active probiotics help regulate normal vaginal flora and restore damaged microecology, and freeze-dried active bacteria can effectively prolong the survival rate and survival period of probiotics.

[0004] Therefore, a gynecological probiotic freeze-dried active bacteria antibacterial tablet and a preparation method are provided. Summary of the invention

[0005] The purpose of the present invention is to overcome the existing defects and provide a gynecological probiotic freeze-dried active bacteria antibacterial tablet and a preparation method, which effectively prolongs the survival rate and survival period of probiotics and improves the microecological balance, thereby preventing the growth of probiotics.

[0006] The technical solution to achieve the above purpose is: The present invention discloses a gynecological probiotic freeze-dried active bacteria antibacterial tablet, which specifically comprises the following components in parts by weight: probiotic live bacteria, freeze-drying protective agent, soybean polypeptide powder, montmorillonite, D-mannitol, oligofructose, sorbitol, microcrystalline cellulose, chlorhexidine acetate and magnesium stearate.

[0007] Preferably, the live probiotic bacteria include lactobacillus and / or bifidobacterium, wherein the lactobacillus is one or more of Lactobacillus crispatus, Lactobacillus acidophilus, Lactobacillus jensenii, Lactobacillus gasseri, Lactobacillus salivarius, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus plantarum, and Lactobacillus bulgaricus.

[0008] Preferably, if the live probiotic bacteria consist of Lactobacillus and Bifidobacterium, the mass ratio of the two is 1:1.

[0009] Preferably, the mass ratio of the live probiotic bacteria, D-mannitol and lyophilization protectant is 6:3:2.

[0010] Preferably, the lyoprotectants are glycerol and xylooligosaccharides.

[0011] The second aspect of the present invention is a method for preparing a gynecological probiotic freeze-dried active bacteria antibacterial tablet, comprising: Step S1, activating and expanding the probiotic live bacteria by adding a culture medium containing soybean polypeptide powder, and fermenting for half a day to obtain a fermentation liquid; Step S2, adding a freeze-drying protective agent to the fermentation liquid, mixing evenly, adding montmorillonite, and freeze-drying to obtain a probiotic mixture; Step S3, grinding the probiotic mixture into powder, adding corresponding weights of D-mannitol, oligofructose, sorbitol and microcrystalline cellulose and continuing to stir and mix evenly; Step S4, respectively adding a certain amount of acetic acid chloride and magnesium stearate, mixing evenly, and pressing into tablets to prepare probiotic freeze-dried active bacteria antibacterial tablets.

[0012] Preferably, in step S1, the components of the culture medium include: soy peptone, beef protein powder, yeast extract, sodium acetate, glucose, Tween 80, ammonium citrate, magnesium sulfate and water.

[0013] Preferably, in step S2, freeze-drying is performed at a pressure of 512 Pa for 6 hours, then freeze-dried at a pressure of 256 Pa for 5 hours, and then freeze-dried at a pressure of 103 Pa until a probiotic mixture is obtained.

[0014] The beneficial effects of the present invention are as follows: in the present invention, the probiotic live bacteria are activated and expanded by adding a culture medium containing soybean polypeptide powder, and fermented for half a day after cultivation to obtain a fermentation liquid, a freeze-dried protective agent is added to the fermentation liquid, montmorillonite is added after mixing evenly, and a probiotic mixture is obtained by freeze-drying; the freeze-dried probiotic mixture can be quickly activated and continue to grow in the use environment, quickly stimulate the immune system to maintain the microecological balance, reduce the medication time, and improve the medication efficiency; the freeze-dried protective agent not only protects the freeze-dried vitality of the probiotic bacteria, but the freeze-dried protective agent of the present invention is also added with oligoxylose, which can increase the abundance of beneficial lactobacilli by release and promote growth; the soybean polypeptide powder in the present invention, on the one hand, as a culture medium component On the one hand, it can provide an amino acid source for probiotic live bacteria, and on the other hand, it can be used as a fermentation promoter for probiotic live bacteria to increase the activation speed, and increase the hydrophobicity of the polypeptide powder by coupling thioisocyanate to the amino N-terminus, thereby increasing the affinity with the bacteria and its utilization, and at the same time improving the stability; montmorillonite not only serves as a bacterial carrier, but also supports the biofilm formed by lactobacillus, which can promote the colonization of probiotics including lactobacillus on the mucosa, promote the rapid growth of probiotic populations, and inhibit the activity of pathogenic bacteria; it effectively prolongs the survival rate and survival period of probiotics, effectively reduces the irritation to the vaginal mucosa, is more gentle and safe, and improves the purpose of microecological balance, thereby preventing the growth of original bacteria. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The present invention is a flow chart of a method for preparing a gynecological probiotic freeze-dried active bacteria antibacterial tablet. DETAILED DESCRIPTION

[0016] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0017] The present invention will be further described below in conjunction with the accompanying drawings.

[0018] A gynecological probiotic freeze-dried active bacteria antibacterial tablet specifically comprises the following components in parts by weight: probiotic live bacteria, freeze-drying protective agent, soybean polypeptide powder, montmorillonite, D-mannitol, oligofructose, sorbitol, microcrystalline cellulose, chlorhexidine acetate and magnesium stearate.

[0019] In an embodiment, the live probiotic bacteria include lactobacillus and / or bifidobacterium, wherein the lactobacillus is one or more of Lactobacillus crispatus, Lactobacillus acidophilus, Lactobacillus jensenii, Lactobacillus gasseri, Lactobacillus salivarius, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus plantarum, and Lactobacillus bulgaricus.

[0020] In the embodiment, if the composition of the live probiotic bacteria is Lactobacillus and Bifidobacterium, the mass ratio of the two is 1:1.

[0021] In the embodiment, the mass ratio of the live probiotic bacteria, D-mannitol and the lyophilization protective agent is 6:3:2.

[0022] In the embodiment, the lyoprotectants are glycerol and xylo-oligosaccharides.

[0023] like Figure 1 As shown, a method for preparing a gynecological probiotic freeze-dried active bacteria antibacterial tablet comprises: Step S1, activating and expanding the probiotic live bacteria by adding a culture medium containing soybean polypeptide powder, and fermenting for half a day after the culture to obtain a fermentation liquid.

[0024] In the embodiment, the components of the culture medium include: soy peptone, beef protein powder, yeast extract, sodium acetate, glucose, Tween 80, ammonium citrate, magnesium sulfate and water.

[0025] In the embodiments, the soybean polypeptide powder, on the one hand, as a culture medium component, provides an amino acid source for probiotic live bacteria, and on the other hand, can be used as a fermentation promoter for probiotic live bacteria to increase the activation rate, and the hydrophobicity of the polypeptide powder is increased by coupling thioisocyanate to the amino N-terminus, thereby increasing the affinity with the bacteria and its utilization, and at the same time improving the stability.

[0026] Step S2, adding a freeze-drying protective agent to the fermentation liquid, mixing evenly, adding montmorillonite, and freeze-drying to obtain a probiotic mixture.

[0027] In the embodiment, the lyophilization protectant not only protects the lyophilization activity of the probiotic bacteria, but also contains xylooligosaccharides, which can increase the abundance of beneficial lactobacilli through release and promote their growth.

[0028] In the embodiments, montmorillonite not only serves as a bacterial carrier, but also supports the biofilm formed by lactobacillus, thereby promoting the colonization of probiotics including lactobacillus on the mucosa, promoting the rapid growth of probiotic populations, and inhibiting the activity of pathogenic bacteria.

[0029] In the example, freeze drying is performed at a pressure of 512 Pa for 6 h, followed by freeze drying at a pressure of 256 Pa for 5 h, and then freeze drying is performed at a pressure of 103 Pa until a probiotic mixture is obtained.

[0030] Step S3, grinding the probiotic mixture into powder, adding corresponding weights of D-mannitol, oligofructose, sorbitol and microcrystalline cellulose and continuing to stir and mix evenly.

[0031] Step S4, respectively adding a certain amount of acetic acid chloride and magnesium stearate, mixing evenly, and pressing into tablets to prepare probiotic freeze-dried active bacteria antibacterial tablets.

[0032] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions described in the above embodiments may still be modified, or some or all of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A gynecological probiotic freeze-dried active bacteria antibacterial tablet, characterized in that: The invention specifically comprises the following components in parts by weight: live probiotic bacteria, freeze-drying protective agent, soybean polypeptide powder, montmorillonite, D-mannitol, oligofructose, sorbitol, microcrystalline cellulose, chlorhexidine acetate and magnesium stearate.

2. The gynecological probiotic freeze-dried active bacteria antibacterial tablet according to claim 1, characterized in that: The live probiotic bacteria include lactobacillus and / or bifidobacterium, wherein the lactobacillus is one or more of Lactobacillus crispatus, Lactobacillus acidophilus, Lactobacillus jensenii, Lactobacillus gasseri, Lactobacillus salivarius, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus plantarum, and Lactobacillus bulgaricus.

3. The gynecological probiotic freeze-dried active bacteria antibacterial tablet according to claim 1, characterized in that: If the live probiotic bacteria consist of Lactobacillus and Bifidobacterium, the mass ratio of the two is 1:

1.

4. The gynecological probiotic freeze-dried active bacteria antibacterial tablet according to claim 1, characterized in that: The mass ratio of the live probiotic bacteria, D-mannitol and freeze-drying protective agent is 6:3:

2.

5. The gynecological probiotic freeze-dried active bacteria antibacterial tablet according to claim 1, characterized in that: The freeze-drying protective agents are glycerol and xylooligosaccharide.

6. A method for preparing a gynecological probiotic freeze-dried active bacteria antibacterial tablet, characterized in that: include: Step S1, activating and expanding the probiotic live bacteria by adding a culture medium containing soybean polypeptide powder, and fermenting for half a day to obtain a fermentation liquid; Step S2, adding a freeze-drying protective agent to the fermentation liquid, mixing evenly, adding montmorillonite, and freeze-drying to obtain a probiotic mixture; Step S3, grinding the probiotic mixture into powder, adding corresponding weights of D-mannitol, oligofructose, sorbitol and microcrystalline cellulose and continuing to stir and mix evenly; Step S4, respectively adding a certain amount of acetic acid chloride and magnesium stearate, mixing evenly, and pressing into tablets to prepare probiotic freeze-dried active bacteria antibacterial tablets.

7. The method for preparing a gynecological probiotic freeze-dried active bacteria antibacterial tablet according to claim 6, characterized in that: In step S1, the components of the culture medium include: soy peptone, beef protein powder, yeast extract, sodium acetate, glucose, Tween 80, ammonium citrate, magnesium sulfate and water.

8. The method for preparing a gynecological probiotic freeze-dried active bacteria antibacterial tablet according to claim 6, characterized in that: In step S2, freeze drying is performed at a pressure of 512 Pa for 6 hours, then freeze drying is performed at a pressure of 256 Pa for 5 hours, and then freeze drying is performed at a pressure of 103 Pa until a probiotic mixture is obtained.

Images