Separation method for alkaline microorganism inspection
By breaking and screening the soil using a crushing assembly and screening device, the problem of the impact of agglomeration and small stones during soil mixing is solved, and rapid, uniform soil mixing and efficient separation results are achieved.
Patent Information
- Application Number
- CN202411583290.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-07
- Publication Date
- 2025-02-07
AI Technical Summary
In the prior art, the agglomeration of soil during mixing leads to a long time and insufficient mixing, and small stones are prone to damage the culture medium and affect the separation results.
Using a device including a crushing assembly and a screen, the soil samples are placed in the feed silo, the soil is broken through the crushing assembly, and then the larger particles and small stones are screened through the screen to obtain powdery soil for easy and non-existence. Mix bacteria water.
The soil mixing time is reduced, the mixing effect is improved, the small stones are avoided on the quality of the sample, and the accuracy of the separation results of alkaline microbial tests is improved.
Smart Images
Figure CN119391815A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of microbial separation, and in particular to a separation method for alkaline microbial testing. Background Art
[0002] Alkaline microorganisms refer to those that can survive and even thrive in an alkaline environment. The main separation methods for alkaline microorganism testing are: Pour plate method: dilute the microbial suspension through a series of dilutions, then add the diluted sample to the sterile culture medium and mix evenly. After solidification, invert the culture. After multiple proliferations, a single cell forms a colony. Take a single colony to make a suspension. Repeat the above steps several times to obtain a pure culture; Spread plate method: Move a certain concentration and a certain amount of the to-be-separated bacterial solution to a solidified culture medium plate, and then quickly and evenly spread it with a spreading rod to grow a single colony to achieve the purpose of separation; Streak plate method: Line method: Use a sterile inoculation loop to take a small amount of culture and streak it on the plate. There are many ways to streak, the most common ones are the oblique line method, the curve method, the grid method, the radial method, the four-grid method, etc. Through the streaking method, a plate is divided into small areas of different areas for streaking. Finally, single cells are scattered on the streaked lines. After cultivation, each cell grows into a colony; Enrichment culture method: Starting from a mixed group of microorganisms, create some conditions to allow only the desired microorganisms to grow. Under these conditions, the desired microorganisms can effectively compete with other microorganisms, leading to pure culture. Regardless of which method is used, the sample used is usually prepared before the operation. At present, when preparing samples, the soil is usually placed in a beaker, and then sterile water is added for stirring and mixing. During this process, when the soil is in block form or contains lumps of soil, the stirring time will be long and the mixing effect will be poor. And when the soil contains small stones, it is not easy to find that the stones will not only hit the beaker during stirring, easily causing damage to the beaker, but also affect the quality of sample preparation and the inspection and separation effect. Summary of the invention
[0003] The purpose of the present invention is to solve the problems in the prior art that the mixing time of agglomerated soil is long, the mixing is insufficient, and the small stones contained in the soil will affect the separation result, and a separation method for alkaline microorganism testing is proposed.
[0004] In order to achieve the above object, the present invention adopts the following technical solutions: A separation method for alkaline microbial testing comprises the following steps: Step 1: Select appropriate samples for collection based on the type of microorganisms that need to be isolated; Step 2: Properly process the collected samples; Step 3: Inoculate the treated sample onto sterile culture medium and separate it using the plate streak method; Step 4: Place the inoculated culture medium under suitable temperature and conditions for cultivation, and observe and record the formation of colonies; Step 5: Through repeated separation and purification operations, pure culture is finally obtained.
[0005] In order to facilitate the preparation of samples, preferably, it also includes a box body, a feed bin is fixedly arranged on the top of the box body, a crushing assembly is arranged in the feed bin, a screening frame is slidably arranged in the box body, a screen is arranged in the screening frame, and the output end of the feed bin is connected to the screening frame.
[0006] In order to facilitate the breaking up of the soil, the crushing assembly further includes a crushing shaft rotatably arranged in the feed bin, a first crushing knife is fixedly arranged on the crushing shaft, and a second crushing knife corresponding to the first crushing knife is fixedly arranged on the inner wall of the feed bin, a guide rod is arranged in the box body, a guide groove is opened at one end of the crushing shaft, one end of the guide rod is slidably connected to the guide groove, a first spring is arranged in the guide groove, and the two ends of the first spring are respectively against the guide groove and the guide rod.
[0007] In order to facilitate the screening of the dispersed soil, a screening cavity is further provided in the box body, the screening frame is slidably arranged in the screening cavity, the screening cavity is connected with the feed bin through a feeding channel, and a connecting shaft is fixedly provided at one end of the screen, and the connecting shaft is rotatably connected to one end of the screening frame through a torsion spring.
[0008] Furthermore, a pressing plate is fixedly provided at the bottom of the crushing shaft, a push rod is lifted and lowered at the other end of the screening frame, a sphere is fixedly provided at one end of the push rod, the sphere abuts against the other end of the screen, and a top plate is fixedly provided at the other end of the push rod, the top of the top plate abuts against the bottom of the pressing plate, a second spring is sleeved on the push rod, and both ends of the second spring abut against the top plate and the screening frame respectively.
[0009] Furthermore, a rotating shaft is rotatably arranged in the box body, one end of the rotating shaft is fixedly connected to the guide rod through a crankshaft, and a rotating rod is fixedly arranged at the other end of the screening frame, a connecting rod is rotatably arranged on the rotating rod, and one end of the connecting rod away from the rotating rod is rotatably connected to the crankshaft.
[0010] Furthermore, a discharge chamber connected to the screening chamber is opened in the box body, a connecting cylinder is rotatably arranged at the bottom of the discharge chamber, a mixing cylinder is arranged at the bottom of the connecting cylinder, a first gear is fixedly arranged at the other end of the rotating shaft, a rotating ring is fixedly arranged at the top of the connecting cylinder, an intermediate gear is rotatably arranged in the box body, the intermediate gear is meshed with the first gear, and the intermediate gear is meshed with the outer wall of the rotating ring.
[0011] Furthermore, a support plate is fixedly provided in the discharge chamber, a motor is fixedly provided on the support plate, a stirring shaft is fixedly provided at the output end of the motor, a stirring rod is fixedly provided on the stirring shaft, and a second gear is fixedly provided on the stirring shaft, a third gear is rotatably provided on the support plate, and the third gear is respectively meshed with the second gear and the inner wall of the rotating ring.
[0012] Furthermore, a through slot is provided in the third gear, a plurality of wind blades are fixedly arranged in the through slot, an exhaust pipe is fixedly arranged on the inner wall of the discharge cavity, one end of the exhaust pipe is connected to the through slot, and a through hole is provided at the other end of the exhaust pipe.
[0013] Furthermore, a connecting plate is fixedly provided at the bottom of the connecting cylinder, the inner wall of the mixing cylinder is threadedly connected to the outer wall of the connecting plate, a sealing gasket is fixedly provided at the bottom of the connecting plate, the sealing gasket abuts against the mixing cylinder, and a stirring plate is fixedly provided at the bottom of the mixing cylinder.
[0014] Compared with the prior art, the present invention provides a separation method for alkaline microorganism testing, which has the following beneficial effects: 1. The separation method for alkaline microorganism testing can break up the soil by placing the soil sample in a feed bin and starting the crushing component to reduce nodules or lumps. After the breaking up, the soil will fall on the screen, and the screen can screen the soil. Larger particles of soil or small stones or weeds will be intercepted on the screen. On the one hand, the powdered soil helps to quickly mix with sterile water, reduce the stirring time, and improve the mixing effect. On the other hand, it can reduce the influence of stones and weeds on the sample quality and improve the accuracy of the separation result. 2. The separation method for alkaline microorganism inspection is convenient for screening soil by setting the screening frame to slide back and forth in the screening chamber, and the screen is rotatably set on one end of the screening frame by a connecting shaft. When the crushing shaft slides downward, the screen will rotate downward around the connecting shaft, and the stones and other debris trapped on the screen can be dumped into the collection box, which is convenient for subsequent collection and improves the use effect; 3. The separation method for alkaline microorganism testing is characterized in that a wind blade is fixedly arranged on the third gear. When the third gear rotates, the wind blade generates wind in the exhaust pipe, and then blows it out from the through hole along the exhaust pipe and blows it toward the screening chamber, so that weeds on the screen can be lifted up, thereby reducing blockage of the screen and improving screening efficiency.
[0015] The parts not involved in the device are the same as the prior art or can be implemented by the prior art. The present invention can break up the soil and reduce nodules or lumps by placing the soil sample in the feed bin and starting the crushing component. After the breaking up, the soil will fall on the screen, and the screen can screen the soil. Larger particles of soil or small stones or weeds will be intercepted on the screen. On the one hand, the powdered soil helps to mix quickly with sterile water, reduce the stirring time, and improve the mixing effect. On the other hand, it can reduce the influence of stones and weeds on the sample quality and improve the accuracy of the separation result. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 A schematic diagram of the structure of a separation method for alkaline microorganism testing proposed by the present invention; Figure 2 A cross-sectional view of a separation method for alkaline microorganism testing proposed by the present invention; Figure 3 A separation method for alkaline microorganism testing proposed by the present invention Figure 2 A magnified view of part A; Figure 4 A separation method for alkaline microorganism testing proposed by the present invention Figure 2 A magnified view of part B; Figure 5 This is a schematic diagram of the structure of a wind blade in a separation method for alkaline microorganism testing proposed by the present invention; Figure 6 This is a schematic diagram of the structure of a discharge chamber of a separation method for alkaline microorganism testing proposed by the present invention; Figure 7 This is a schematic diagram of the structure of a crushing shaft of a separation method for alkaline microorganism testing proposed by the present invention.
[0017] In the figure: 1. Box; 101. Feeding channel; 102. Screening chamber; 103. Discharging chamber; 104. Support legs; 2. Feeding bin; 201. Crushing shaft; 202. First crushing knife; 203. Comb teeth; 204. Guide rod; 205. First spring; 206. Pressing plate; 207. Crankshaft; 208. Rotating shaft; 209. First gear; 3. Screening frame; 301. Screen; 302. Connecting shaft; 303. Rotating rod; 304. Ejector rod ; 305, top plate; 306, sphere; 307, second spring; 308, connecting rod; 4, stirring shaft; 401, stirring rod; 402, motor; 403, second gear; 404, support plate; 5, third gear; 501, wind blade; 502, exhaust pipe; 503, through hole; 6, connecting cylinder; 601, rotating ring; 602, connecting plate; 7, mixing cylinder; 701, stirring plate; 8, collecting box; 801, baffle; 802, handle. DETAILED DESCRIPTION
[0018] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.
[0019] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. Example
[0020] Reference Figure 1-Figure 7 , a separation method for alkaline microorganism testing, comprising the following steps: Step 1: Select appropriate samples for collection based on the type of microorganisms that need to be isolated; Before the operation, first use a certain amount of soil at the target location. During the transfer, when the distance is long, it is best to store it in an incubator or refrigerator at 4°C; Step 2: Properly process the collected samples; The collected specimens are processed, such as dilution and centrifugation. Here, we break up the soil to reduce nodules or lumps, then screen it to remove small stones and weeds in the soil, and finally mix the processed soil with sterile water for subsequent use; Step 3: Inoculate the treated sample onto sterile culture medium and separate it using the plate streak method; After sample preparation, the sample was inoculated onto sterile culture medium using the plate streak method; Step 4: Place the inoculated culture medium under suitable temperature and conditions for cultivation, and observe and record the formation of colonies; The culture medium is placed in a constant temperature box, the temperature is usually 28-37°C, and a high pH value (usually pH 9-11) is maintained. The culture time is determined according to the growth rate of the microorganism, generally 24-72 hours; Step 5: Through repeated separation and purification operations, pure culture is finally obtained.
[0021] Record the morphological characteristics of the colonies on the plate, such as color, size, shape, etc., select single colonies with obvious morphological characteristics for further purification, and finally store the purified strains in slant culture medium or freezing preservation solution for subsequent use.
[0022] Reference Figure 1 and Figure 2 When processing and preparing samples, a box 1 is used. A feed bin 2 is fixedly arranged on the top of the box 1, a crushing assembly is arranged in the feed bin 2, and a support leg 104 is fixedly arranged at the bottom of the box 1 to support the support leg 104. A screening frame 3 is slidably arranged in the box 1, and a screen 301 is arranged in the screening frame 3 to connect the output end of the feed bin 2 with the screening frame 3. Before and after use, the box 1 and all parts in the box 1 are disinfected and sterilized. It is preferred to use high temperature and ultraviolet irradiation to sterilize, so as to reduce the risk of sample When in use, by placing the soil sample into the feed bin 2 and starting the crushing assembly, the soil can be broken up to reduce nodules or lumps, and then the soil will fall on the screen 301 after the breaking up. The screen 301 can screen the soil, and larger particles of soil or small stones or weeds will be intercepted on the screen 301. On the one hand, the powdered soil helps to quickly mix with sterile water, reduce the stirring time, and improve the mixing effect. On the other hand, it can reduce the influence of stones and weeds on the sample quality and improve the accuracy of the separation result.
[0023] Reference Figure 2 and Figure 7 Here, we design the crushing assembly as follows: a crushing shaft 201 is rotatably arranged in the feed bin 2, a first crushing knife 202 is fixedly arranged on the crushing shaft 201, two to eight groups of the first crushing knives 202 are arranged, preferably five groups, each group is provided with two first crushing knives 202, and the five groups are equidistant along the axis of the crushing shaft 201, and a second crushing knife corresponding to the first crushing knife 202 is fixedly arranged on the inner wall of the feed bin 2, and a comb tooth 203 is provided on the top first crushing knife 202 to facilitate the collection of weeds and the like in the soil and improve the use effect, a guide rod 204 is arranged in the box body 1, a guide groove is provided at one end of the crushing shaft 201, one end of the guide rod 204 is slidably connected to the guide groove, a first spring 205 is provided in the guide groove, and a first spring 205 is provided in the first The two ends of a spring 205 are respectively against the guide groove and the guide rod 204. The cross-section of the guide rod 204 is polygonal, preferably hexagonal. When in use, the guide rod 204 drives the crushing shaft 201 to rotate with the first crushing knife 202 to break up and crush the soil, which helps to make the soil powdery. Moreover, when the first crushing knife 202 encounters a stone, the first crushing knife 202 can slide downward along the guide rod 204 to stagger the stone from the first crushing knife 202. On the one hand, it can reduce the wear of the first crushing knife 202 and increase its service life. On the other hand, when the first crushing knife 202 slides downward, the soil at the bottom of the feed bin 2 can be broken up again to improve the crushing effect. Moreover, it can avoid breaking up the stone as much as possible to prevent affecting the inspection data and improve the use effect.
[0024] Reference Figure 2 A screening chamber 102 is provided in the box body 1, the screening frame 3 is slidably arranged in the screening chamber 102, and the screening chamber 102 is connected with the feed bin 2 through the material discharge channel 101, and a connecting shaft 302 is fixedly arranged on one end of the screen 301, and the connecting shaft 302 is rotatably connected to one end of the screening frame 3 through a torsion spring, and a collecting box 8 for collecting small stones and other debris is slidably arranged on the box body 1, and the collecting box 8 is connected with the other end of the screening frame 3, and a baffle 801 is fixedly arranged on one end of the collecting box 8, and a baffle 801 is fixedly arranged on the baffle 8. A handle 802 is fixedly provided on the plate 801, which is convenient for installing the collection box 8 on the box body 1. When in use, the screening frame 3 is reciprocatingly slidable in the screening chamber 102 to facilitate screening of the soil, and the screen 301 is rotatably set on one end of the screening frame 3 using the connecting shaft 302. When the crushing shaft 201 slides downward, it will carry the screen 301 and rotate downward around the connecting shaft 302, and the stones and other debris trapped on the screen 301 can be dumped into the collection box 8, which is convenient for subsequent collection and improves the use effect.
[0025] Reference Figure 2 and Figure 4 A pressing plate 206 is fixedly arranged at the bottom of the crushing shaft 201, and a push rod 304 is lifted and lowered at the other end of the screening frame 3. The cross section of the push rod 304 is also polygonal, preferably hexagonal. A sphere 306 is fixedly arranged on one end of the push rod 304, and the sphere 306 is abutted against the other end of the screen 301. A top plate 305 is fixedly arranged on the other end of the push rod 304, and the top of the top plate 305 is abutted against the bottom of the pressing plate 206, and a sleeve is arranged on the push rod 304. There is a second spring 307, and the two ends of the second spring 307 are respectively pressed against the top plate 305 and the screening frame 3. When in use, a top rod 304 is raised and lowered on the screening frame 3. When the crushing shaft 201 slides downward with the pressing plate 206, the top plate 305 can push the top rod 304 and the ball 306 to slide downward, thereby driving the screen 301 to rotate downward around the connecting shaft 302, so that the stones and other debris trapped on the screen 301 can be cleaned up, thereby improving the use effect.
[0026] Reference Figure 2 and Figure 7A rotating shaft 208 is rotatably arranged in the box body 1, one end of the rotating shaft 208 is fixedly connected to the guide rod 204 through a crankshaft 207, and a rotating rod 303 is fixedly arranged at the other end of the screening frame 3, and a connecting rod 308 is rotatably arranged on the rotating rod 303, and one end of the connecting rod 308 away from the rotating rod 303 is rotatably connected to the crankshaft 207. When in use, when the rotating shaft 208 rotates with the crushing shaft 201 through the crankshaft 207 for crushing, the screening frame 3 can be driven to slide back and forth in the screening chamber 102 through the crankshaft 207 and the connecting rod 308, so as to screen the soil and improve the use effect.
[0027] Reference Figure 2-Figure 7 A discharge chamber 103 connected with the screening chamber 102 is provided in the casing 1, and the discharge chamber 103 is in a trumpet-shaped structure. A connecting cylinder 6 is rotatably provided at the bottom of the discharge chamber 103, a mixing cylinder 7 is provided at the bottom of the connecting cylinder 6, and a first gear 209 is fixedly provided on the other end of the rotating shaft 208, and a rotating ring 601 is fixedly provided on the top of the connecting cylinder 6. An intermediate gear is rotatably provided in the casing 1, and the intermediate gear is meshed with the first gear 209, and the intermediate gear is meshed with the outer wall of the rotating ring 601. When in use, the soil screened by the screen 301 will fall into the discharge chamber 103, and then pass through the connecting cylinder 6 to enter the mixing cylinder 7 for mixing. At this time, driving the connecting cylinder 6 to rotate can not only drive the mixing cylinder 7 to rotate and mix, but also drive the rotating shaft 208 to rotate through the rotating ring 601, the intermediate gear and the first gear 209, thereby improving the use effect.
[0028] Reference Figure 2-Figure 7 A support plate 404 is fixedly arranged in the discharge chamber 103, a motor 402 is fixedly arranged on the support plate 404, a stirring shaft 4 is fixedly arranged on the output end of the motor 402, the stirring shaft 4 extends into the mixing drum 7, a stirring rod 401 is fixedly arranged on the stirring shaft 4, for convenient stirring in the mixing drum 7, and a second gear 403 is fixedly arranged on the stirring shaft 4, a third gear 5 is rotatably arranged on the support plate 404, and at least one group of the third gear 5 is arranged, and here, we preferably have two groups, and the two groups of the third gear 5 are respectively meshed with the second gear 403 and the inner wall of the rotating ring 601. When in use, the stirring shaft 4 is driven by the motor 402 to carry the stirring rod 401 to stir in the mixing drum 7, for convenient mixing, and the connecting drum 6 and the mixing drum 7 can be driven to rotate in the opposite direction through the third gear 5, so as to further improve the mixing effect.
[0029] Reference Figure 3 , Figure 5 and Figure 6A through slot is provided in the third gear 5, and a plurality of groups of wind blades 501 are fixedly arranged in the through slot. The wind blades 501 are provided with two to ten groups, preferably six groups, which are evenly distributed in the through slot. An exhaust cylinder 502 is fixedly provided on the inner wall of the discharge chamber 103, and one end of the exhaust cylinder 502 is connected to the through slot, and a through hole 503 is provided at the other end of the exhaust cylinder 502. When in use, the wind blades 501 are fixedly provided on the third gear 5. When the third gear 5 rotates, the wind blades 501 generate wind in the exhaust cylinder 502, and then blown out from the through hole 503 along the exhaust cylinder 502 and blown toward the screening chamber 102, which can make the weeds on the screen 301 float up, reduce the blockage of the screen 301, and improve the screening efficiency.
[0030] Reference Figure 2 and Figure 3 A connecting plate 602 is fixedly provided at the bottom of the connecting cylinder 6, and the inner wall of the mixing cylinder 7 is threadedly connected to the outer wall of the connecting plate 602, which is convenient for assembly and disassembly and reliable for fixation. A sealing gasket is fixedly provided at the bottom of the connecting plate 602, and the sealing gasket is abutted against the mixing cylinder 7 to improve the sealing effect, and a stirring plate 701 is fixedly provided at the inner bottom of the mixing cylinder 7. When the mixing cylinder 7 rotates, the stirring effect can be improved. When in use, the mixing cylinder 7 is threadedly connected to the connecting cylinder 6, which makes it easy to assemble and disassemble the mixing cylinder 7, and the stirring plate 701 is fixedly provided in the mixing cylinder 7 to further improve the mixing effect.
[0031] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.
Claims
1. A separation method for alkaline microorganism testing, characterized in that: The following steps are involved: Step 1: Select appropriate samples for collection based on the type of microorganisms that need to be isolated; Step 2: Properly process the collected samples; Step 3: Inoculate the treated sample onto sterile culture medium and separate it using the plate streak method; Step 4: Place the inoculated culture medium under suitable temperature and conditions for cultivation, and observe and record the formation of colonies; Step 5: Through repeated separation and purification operations, pure culture is finally obtained.
2. A separation method for alkaline microorganism testing according to claim 1, characterized in that: It also comprises a box body (1), a feed bin (2) being fixedly arranged on the top of the box body (1), a crushing assembly being arranged in the feed bin (2), a screening frame (3) being slidably arranged in the box body (1), a screen (301) being arranged in the screening frame (3), and an output end of the feed bin (2) being connected to the screening frame (3).
3. A separation method for alkaline microorganism testing according to claim 2, characterized in that: The pulverizing assembly comprises a pulverizing shaft (201) rotatably arranged in the feed bin (2), a first pulverizing blade (202) being fixedly arranged on the pulverizing shaft (201), and a second pulverizing blade corresponding to the first pulverizing blade (202) being fixedly arranged on the inner wall of the feed bin (2), a guide rod (204) being arranged in the housing (1), a guide groove being formed at one end of the pulverizing shaft (201), one end of the guide rod (204) being slidably connected to the guide groove, a first spring (205) being arranged in the guide groove, and two ends of the first spring (205) respectively abutting against the guide groove and the guide rod (204).
4. A separation method for alkaline microorganism testing according to claim 3, characterized in that: A screening chamber (102) is provided in the housing (1), the screening frame (3) is slidably arranged in the screening chamber (102), the screening chamber (102) is connected to the feed bin (2) via a feed channel (101), and a connecting shaft (302) is fixedly arranged at one end of the screen (301), and the connecting shaft (302) is rotatably connected to one end of the screening frame (3) via a torsion spring.
5. A separation method for alkaline microorganism testing according to claim 4, characterized in that: A pressing plate (206) is fixedly provided at the bottom of the crushing shaft (201); a lifting rod (304) is lifted and lowered at the other end of the screening frame (3); a sphere (306) is fixedly provided at one end of the lifting rod (304); the sphere (306) abuts against the other end of the screen (301); a top plate (305) is fixedly provided at the other end of the lifting rod (304); the top of the top plate (305) abuts against the bottom of the pressing plate (206); a second spring (307) is sleeved on the lifting rod (304); two ends of the second spring (307) abut against the top plate (305) and the screening frame (3) respectively.
6. A separation method for alkaline microorganism testing according to claim 5, characterized in that: A rotating shaft (208) is rotatably arranged in the box body (1), one end of the rotating shaft (208) is fixedly connected to the guide rod (204) via a crankshaft (207), and a rotating rod (303) is fixedly arranged at the other end of the screening frame (3), a connecting rod (308) is rotatably arranged on the rotating rod (303), and one end of the connecting rod (308) away from the rotating rod (303) is rotatably connected to the crankshaft (207).
7. A separation method for alkaline microorganism testing according to claim 6, characterized in that: The housing (1) is provided with a discharge chamber (103) which is in communication with the screening chamber (102); a connecting cylinder (6) is rotatably arranged at the bottom of the discharge chamber (103); a mixing cylinder (7) is arranged at the bottom of the connecting cylinder (6); a first gear (209) is fixedly arranged at the other end of the rotating shaft (208); a rotating ring (601) is fixedly arranged at the top of the connecting cylinder (6); an intermediate gear is rotatably arranged in the housing (1); the intermediate gear is meshed with the first gear (209); and the intermediate gear is meshed with the outer wall of the rotating ring (601).
8. A separation method for alkaline microorganism testing according to claim 7, characterized in that: A support plate (404) is fixedly arranged in the discharge cavity (103), a motor (402) is fixedly arranged on the support plate (404), a stirring shaft (4) is fixedly arranged at the output end of the motor (402), a stirring rod (401) is fixedly arranged on the stirring shaft (4), and a second gear (403) is fixedly arranged on the stirring shaft (4), a third gear (5) is rotatably arranged on the support plate (404), and the third gear (5) is respectively meshed with the second gear (403) and the inner wall of the rotating ring (601).
9. A separation method for alkaline microorganism testing according to claim 8, characterized in that: A through slot is provided in the third gear (5), and a plurality of groups of wind blades (501) are fixedly arranged in the through slot. An exhaust pipe (502) is fixedly arranged on the inner wall of the discharge cavity (103), one end of the exhaust pipe (502) is connected to the through slot, and a through hole (503) is provided at the other end of the exhaust pipe (502).
10. A separation method for alkaline microorganism testing according to claim 9, characterized in that: A connecting plate (602) is fixedly arranged at the bottom of the connecting cylinder (6), the inner wall of the mixing cylinder (7) is threadedly connected to the outer wall of the connecting plate (602), a sealing gasket is fixedly arranged at the bottom of the connecting plate (602), the sealing gasket abuts against the mixing cylinder (7), and a stirring plate (701) is fixedly arranged at the inner bottom of the mixing cylinder (7).
Citation Information
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