Small dog feed additive and preparation method thereof

Abstract

The invention relates to the technical field of feed additives, and particularly discloses a small dog feed additive and a preparation method thereof. The small dog feed additive comprises an additive main material and a coating layer used for wrapping the additive main material, the coating layer and the additive main material form a microcapsule with a core-shell structure, and the coating layer comprises the following raw materials: 40-50 parts of lecithin, 4-8 parts of a film-forming agent, 4-8 parts of a structure enhancer and 2-4 parts of a natural antioxidant. The preparation method comprises the following steps: adding the additive main material into hydrated liquid, and uniformly dispersing to obtain a water-phase additive; the preparation method comprises the following steps: adding lecithin, a film-forming agent, a structure enhancer and a natural antioxidant into an organic solvent, uniformly dispersing, and uniformly mixing with a water-phase additive to obtain a turbid solution; and evaporating to remove the organic solvent in the turbid liquid, and then centrifuging, carrying out solid-liquid separation and drying to obtain the small dog feed additive. According to the invention, the absorption effect of the small dogs on nutritional substances in the main materials of the additive can be improved.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, in particular to a feed additive for small dogs and a preparation method thereof. Background Art

[0002] With the continuous development of the pet market and people's increasing attention to pet health, the research and application of small dog feed additives has gradually become a hot topic in the industry. As an important means to improve the nutritional value of feed and improve the health of pets, the continuous innovation of additives in types and preparation methods has brought more possibilities for the breeding and management of small dogs.

[0003] Small dog feed additives mainly include two categories: nutritional additives and non-nutritional additives. Nutritional additives such as amino acids, vitamins, minerals, etc. are designed to supplement and improve the nutritional components in feed to meet the growth and development and daily activities of small pet dogs. Non-nutritional additives include enzyme preparations, probiotics, Chinese herbal medicines, etc. They mainly play a role in promoting health, preventing and curing diseases by regulating the physiological functions of small pet dogs, improving feed quality or extending shelf life.

[0004] Since small dogs have relatively small stomachs, short digestion times, and faster food digestion rates, food and feed additives will be quickly digested in the bodies of small dogs. The digested food and feed additives will mix together and enter the intestines, resulting in a lower concentration of feed additives in the intestines. The intestines absorb the nutritional additives in the feed additives more slowly, and the nutritional additives will be excreted before they have time to be fully absorbed by the intestines of small dogs, causing waste. Summary of the invention

[0005] In order to improve the absorption effect of feed additives for small dogs, the present application provides a feed additive for small dogs and a preparation method thereof.

[0006] In the first aspect, the present application provides a small dog feed additive, which adopts the following technical solution:

[0007] A small dog feed additive comprises an additive main ingredient and a coating layer for wrapping the additive main ingredient, wherein the coating layer and the additive main ingredient form a microcapsule with a core-shell structure, and the coating layer comprises the following raw materials: 40-50 parts of lecithin, 4-8 parts of a film former, 4-8 parts of a structure enhancer and 2-4 parts of a natural antioxidant.

[0008] By adopting the above technical scheme, lecithin as the main component of the coating layer has good biocompatibility and self-assembly ability, can form a stable double-layer membrane structure, effectively wrap the main ingredient of the additive, reduce the damage of the main ingredient of the additive by the digestive enzymes in the stomach and intestines, and help the main ingredient of the additive to be released in the intestine of small dogs, so that the nutrients in the main ingredient of the additive are maintained at a high concentration in the intestine, and the absorption effect of the nutrients in the main ingredient of the additive by small dogs is improved. The film-forming agent can enhance the stability of the coating layer and ensure the integrity of the microcapsules during the processing. The structural enhancer can enhance the structural stability of the microcapsules so that the main ingredient of the additive can be released in the intestine, thereby improving its absorption efficiency. Natural antioxidants can prevent or reduce oxidation and deterioration of feed additives. Therefore, the small dog feed additive of the present application can improve the absorption effect of small dogs on the nutrients in the main ingredient of the additive.

[0009] In a specific embodiment, the structural reinforcing agent is polyethylene glycol or polyvinyl alcohol.

[0010] By adopting the above technical solution, polyethylene glycol and polyvinyl alcohol can be used in feed additives, have good biocompatibility and low toxicity in animals, and will not have a negative impact on the health of small dogs. Moreover, they can form a complex with lecithin to improve the structural stability of the capsule wall.

[0011] In a specific embodiment, the film-forming agent is chitosan or sodium alginate.

[0012] By adopting the above technical scheme, both chitosan and sodium alginate have excellent film-forming ability, which makes the microcapsule have stronger structural stability. During the processing, transportation and storage of feed, it can reduce the damage of the lecithin layer and the loss of additives. It is safe and harmless to small dogs. It can also reduce the risk of adverse reactions such as lecithin oxidation and hydrolysis, and maintain the activity and stability of feed additives.

[0013] In a specific embodiment, the natural antioxidant is vitamin E or tea polyphenols.

[0014] By adopting the above technical solution, vitamin E and tea polyphenols are both natural antioxidants, which can effectively prevent or reduce the oxidation reaction of lecithin and other sensitive ingredients in feed additives. In addition, vitamin E and tea polyphenols can also promote the absorption of lecithin and other nutrients in feed by small dogs.

[0015] In a specific embodiment, the additive main ingredient includes at least one of amino acids, vitamins, and minerals.

[0016] Amino acids, vitamins and minerals are all commonly used nutritional additives.

[0017] In a specific embodiment, the additive ingredient includes bile acid.

[0018] By adopting the above technical solution, bile acid is an important auxiliary factor for fat digestion, which can improve the digestibility and absorption rate of lecithin in feed additives for small dogs. After the microcapsule structure of the feed additive is broken, small dogs can quickly digest and absorb lecithin and other ingredients wrapped in lecithin, preventing other ingredients wrapped in lecithin from being excreted from the body due to slow rupture of lecithin.

[0019] In a second aspect, the present application provides a method for preparing a small dog feed additive, using the following technical solution:

[0020] A method for preparing a small dog feed additive comprises the following steps:

[0021] Dissolution: Add the main additive material into the hydration liquid and disperse it evenly to obtain the water phase additive;

[0022] Hydration: adding lecithin, film former, structure enhancer and natural antioxidant into organic solvent, dispersing evenly, and then mixing evenly with water phase additive to obtain turbid liquid;

[0023] Purification: Evaporation is performed to remove the organic solvent in the turbid liquid, followed by centrifugation, solid-liquid separation, and drying to obtain a small dog feed additive.

[0024] By adopting the above technical solution, during the dissolution and hydration process, the various components are evenly dispersed and mixed, which helps to improve the uniformity of the various components in the final product. During the hydration process, the lecithin molecules will self-assemble to form a double-layer membrane structure, encapsulating the main additive ingredients. The film-forming agent, structural enhancer and natural antioxidant promote the lecithin to form microcapsules with a core-shell structure. Through evaporation, centrifugation, solid-liquid separation and other steps, the organic solvent and the unencapsulated main additive ingredients are removed, which helps to improve the safety and purity of small dog feed additives.

[0025] In a specific embodiment, the organic solvent is ethanol or ethyl acetate.

[0026] By adopting the above technical scheme, ethanol and ethyl acetate are both polar solvents, which can effectively dissolve additive components such as lecithin, film formers, and structural enhancers, ensuring that each component is evenly dispersed in the solvent, thereby obtaining a stable turbid solution. In the purification step, ethanol and ethyl acetate have high volatility and are more easily removed by evaporation, which helps to simplify the purification process, improve production efficiency, and prevent organic solvent residues in the final product. Moreover, ethanol and ethyl acetate are widely used in the pharmaceutical and food industries, and their toxicity and irritation are relatively low, which can reduce the risk of harm to operators and the environment.

[0027] In a specific embodiment, the hydration fluid is distilled water or physiological saline.

[0028] In a specific embodiment, in the purification step, the organic solvent in the turbid liquid is removed by a rotary evaporation process at 45-55°C, a vacuum degree of -0.08 to -0.1 MPa, and a rotation speed of 20-100 rpm, and centrifugation is performed at a speed of 1000-10000 rpm, and then the liquid after centrifugation is removed, and the solid after centrifugation is dried to obtain a small dog feed additive.

[0029] By adopting the above technical solution, the present applicant has found through experiments that under the above process conditions, microcapsules with a core-shell structure can be prepared to improve the absorption effect of feed additives for small dogs.

[0030] In summary, this application has the following beneficial effects:

[0031] 1. The present application forms microcapsules with a core-shell structure, so that the nutrients in the main additive ingredients maintain a high concentration in the intestine, which can improve the absorption effect of the nutrients in the main additive ingredients by small dogs.

[0032] 2. Bile acid is preferably used in the present application, which can quickly digest and absorb lecithin and other components wrapped in lecithin, and prevent other components wrapped in lecithin from being excreted from the body due to slow rupture of lecithin.

[0033] 3. The method of the present application helps to improve the safety and purity of small dog feed additives. DETAILED DESCRIPTION

[0034] Unless otherwise specified, the raw materials used in the examples and comparative examples of the present application are all purchased from the market. Leucine is food grade L-leucine; lecithin is food grade soybean lecithin; and chitosan is food grade water-soluble chitosan.

[0035] The present application is further described in detail below in conjunction with embodiments and comparative examples.

[0036] Example

[0037] Example 1

[0038] This embodiment provides a small dog feed additive, including an additive main ingredient and a coating layer for coating the additive main ingredient.

[0039] The main ingredients of the additive are as follows: 50g of leucine.

[0040] The coating layer uses the following raw materials: 45g of lecithin, 6g of chitosan, 6g of polyethylene glycol and 3g of vitamin E.

[0041] This embodiment also provides a method for preparing a small dog feed additive, comprising the following steps:

[0042] The main additive material is added into physiological saline, stirred and ultrasonically vibrated to disperse evenly to obtain a water phase additive.

[0043] Lecithin, chitosan, polyethylene glycol and vitamin E are added to ethanol, stirred and then ultrasonically vibrated to disperse evenly, and then, under continuous stirring, an aqueous phase additive is slowly added and stirred until evenly dispersed to obtain a turbid liquid.

[0044] The turbid liquid is added into a rotary evaporator, and the ethanol in the turbid liquid is removed under a rotary evaporation process at 50° C., a vacuum degree of -0.09 MPa, and a rotation speed of 60 rpm, and then centrifuged at a speed of 5500 rpm, and then the liquid after centrifugation is removed, and the solid after centrifugation is dried to obtain a small dog feed additive.

[0045] Example 2

[0046] The only difference between this embodiment and embodiment 1 is that the coating layer uses the following raw materials: 40 g of lecithin, 8 g of chitosan, 8 g of polyethylene glycol and 4 g of vitamin E.

[0047] Example 3

[0048] The only difference between this embodiment and embodiment 1 is that the coating layer uses the following raw materials: 50 g of lecithin, 4 g of chitosan, 4 g of polyethylene glycol and 2 g of vitamin E.

[0049] Example 4

[0050] The difference between this embodiment and embodiment 1 is that polyethylene glycol is replaced by an equal amount of polyvinyl alcohol. In the preparation method of the small dog feed additive, lecithin, chitosan, polyvinyl alcohol and vitamin E are added to ethanol, stirred and ultrasonically vibrated to disperse uniformly, and then, under continuous stirring, the aqueous phase additive is slowly added and stirred to obtain a turbid liquid.

[0051] Example 5

[0052] The difference between this embodiment and embodiment 1 is that chitosan is replaced by an equal amount of sodium alginate. In the preparation method of the small dog feed additive, lecithin, sodium alginate, polyethylene glycol and vitamin E are added to ethanol, stirred and ultrasonically vibrated to disperse uniformly, and then, under continuous stirring, the aqueous phase additive is slowly added and stirred to be uniform to obtain a turbid liquid.

[0053] Example 6

[0054] The only difference between this embodiment and embodiment 1 is that an equal amount of tea polyphenols is used to replace vitamin E. In the preparation method of the small dog feed additive, lecithin, chitosan, polyethylene glycol and tea polyphenols are added to ethanol, stirred and ultrasonically vibrated to disperse evenly, and then, under continuous stirring, the aqueous phase additive is slowly added and stirred to be evenly dispersed to obtain a turbid liquid.

[0055] Example 7

[0056] The difference between this embodiment and embodiment 1 is that the additive main material adopts the following raw materials: 30g of leucine, 10g of vitamin B, and 10g of food-grade calcium carbonate. Leucine, vitamin B, and food-grade calcium carbonate are mixed evenly to obtain the additive main material.

[0057] Example 8

[0058] The difference between this embodiment and embodiment 1 is that the additive main material adopts the following raw materials: 50 g of leucine and 10 g of bile acid. Leucine and bile acid are mixed evenly to obtain the additive main material.

[0059] Example 9

[0060] The difference between this embodiment and embodiment 1 is that the additive main material adopts the following raw materials: 30g of leucine, 10g of vitamin B, 10g of food-grade calcium carbonate, and 10g of bile acid. Leucine, vitamin B, food-grade calcium carbonate and bile acid are mixed evenly to obtain the additive main material.

[0061] Example 10

[0062] The difference between this embodiment and embodiment 1 is that in the preparation method of the small dog feed additive, ethanol is replaced by an equal amount of ethyl acetate. In the preparation method of the small dog feed additive, lecithin, chitosan, polyethylene glycol and vitamin E are added to ethyl acetate, stirred and ultrasonically vibrated to disperse uniformly, and then, under continuous stirring, the aqueous phase additive is slowly added, and stirred to be uniform to obtain a turbid liquid.

[0063] Embodiment 11

[0064] The difference between this embodiment and embodiment 1 is that in the preparation method of the small dog feed additive, an equal amount of distilled water is used to replace the physiological saline. In the preparation method of the small dog feed additive, the main ingredient of the additive is added into distilled water, stirred and ultrasonically vibrated, and dispersed to be uniform to obtain an aqueous phase additive.

[0065] Example 12

[0066] The only difference between this embodiment and embodiment 1 is that, in the preparation method of the small dog feed additive, the turbid liquid is added to a rotary evaporator, and the ethanol in the turbid liquid is removed under a rotary evaporation process at 45° C., a vacuum degree of −0.1 MPa, and a rotation speed of 100 rpm, and then centrifuged at a speed of 1000 rpm, and then the liquid after centrifugation is removed, and the solid after centrifugation is dried to obtain a small dog feed additive.

[0067] Embodiment 13

[0068] The only difference between this embodiment and embodiment 1 is that, in the preparation method of the small dog feed additive, the turbid liquid is added to a rotary evaporator, and the ethanol in the turbid liquid is removed under a rotary evaporation process at 55° C., a vacuum degree of −0.08 MPa, and a rotation speed of 20 rpm, and then centrifuged at a speed of 10,000 rpm, and then the liquid after centrifugation is removed, and the solid after centrifugation is dried to obtain a small dog feed additive.

[0069] Embodiment 14

[0070] The only difference between this embodiment and embodiment 1 is that, in the preparation method of the small dog feed additive, the turbid liquid is added to a rotary evaporator, and the ethanol in the turbid liquid is removed under a rotary evaporation process at 40° C., a vacuum degree of −0.11 MPa, and a rotation speed of 110 rpm, and then centrifuged at a speed of 800 rpm, and then the liquid after centrifugation is removed, and the solid after centrifugation is dried to obtain a small dog feed additive.

[0071] Embodiment 15

[0072] The only difference between this embodiment and embodiment 1 is that, in the preparation method of the small dog feed additive, the turbid liquid is added to a rotary evaporator, and the ethanol in the turbid liquid is removed under a rotary evaporation process at 60° C., a vacuum degree of −0.07 MPa, and a rotation speed of 10 rpm, and then centrifuged at a speed of 11,000 rpm, and then the liquid after centrifugation is removed, and the solid after centrifugation is dried to obtain a small dog feed additive.

[0073] Comparative Example

[0074] Comparative Example 1

[0075] The only difference between this comparative example and Example 1 is that the coating layer uses the following raw materials: 51 g of lecithin, 6 g of polyethylene glycol and 3 g of vitamin E. In the preparation method of the small dog feed additive, lecithin, polyethylene glycol and vitamin E are added to ethanol, stirred and ultrasonically vibrated to disperse evenly, and then, under continuous stirring, the aqueous phase additive is slowly added and stirred to be evenly dispersed to obtain a turbid liquid.

[0076] Comparative Example 2

[0077] The only difference between this comparative example and Example 1 is that the coating layer uses the following raw materials: 51 g of lecithin, 6 g of chitosan and 3 g of vitamin E. Lecithin, chitosan and vitamin E are added to ethanol, stirred and ultrasonically vibrated to disperse them uniformly, and then, under continuous stirring, the aqueous phase additive is slowly added, and stirred to be uniform to obtain a turbid liquid.

[0078] Comparative Example 3

[0079] The difference between this comparative example and Example 1 is that the coating layer uses the following raw materials: 48g of lecithin, 6g of chitosan, and 6g of polyethylene glycol. Lecithin, chitosan, and polyethylene glycol are added to ethanol, stirred, and then ultrasonically vibrated to disperse evenly, and then, under continuous stirring, the aqueous phase additive is slowly added, and stirred until evenly, to obtain a turbid liquid.

[0080] Comparative Example 4

[0081] The only difference between this comparative example and Example 1 is that the coating layer uses the following raw materials: 51 g of chitosan, 6 g of polyethylene glycol and 3 g of vitamin E. Chitosan, polyethylene glycol and vitamin E are added to ethanol, stirred and ultrasonically vibrated to disperse evenly, and then, under continuous stirring, the aqueous phase additive is slowly added, and stirred until evenly dispersed to obtain a turbid liquid.

[0082] Performance testing

[0083] The following performance tests were performed on the small dog feed additives prepared in Examples 1-15 and Comparative Examples 1-4:

[0084] The small dog feed additive and cooked chicken breast were mixed evenly at a mass ratio of 1:200 to obtain dog food. Five-month-old Corgi dogs were used as experimental subjects, and 40 g of dog food was fed at 6:00, 12:00, and 18:00 every day. Feces of the Corgi dogs were collected, and the feces collected on the same day were mixed, and the contents of leucine and lecithin in the feces were detected by high performance liquid chromatography (HPLC). The test results are shown in Table 1.

[0085] Table 1

[0086]

[0087]

[0088] Combining Example 1 and Comparative Examples 1-4 and Table 1, it can be seen that compared with Example 1, the contents of leucine and lecithin in the feces of Comparative Examples 1-4 are significantly increased, which shows that the raw material ratio and preparation method of Example 1 are helpful to improve the absorption effect of leucine and lecithin by Corgi dogs. This shows that the small dog feed additive prepared in Example 1 can improve the absorption effect of small dogs on nutritional substances.

[0089] Combining Examples 1-6, 10-11 and Table 1, it can be seen that the contents of leucine and lecithin in the feces of Examples 1-6 do not change much, and the leucine content is less than 0.3 mg / 100 g, and the lecithin content is less than 0.5 mg / 100 g, which shows that the small dog feed additives prepared using the raw material ratios of Examples 1-6 are all helpful in improving the absorption effect of small dogs on nutrients.

[0090] Combining Examples 1, 7-9 and Table 1, it can be seen that compared with Example 1, the content of leucine in the feces of Example 8 did not change much, but the content of lecithin was significantly reduced. Compared with Example 7, the content of leucine in the feces of Example 9 did not change much, but the content of lecithin was significantly reduced. This shows that the small dog feed additive with added bile acid is helpful to improve the absorption effect of lecithin by small dogs.

[0091] Combining Examples 1, 12-15 and Table 1, it can be seen that compared with Example 1, the leucine content of Examples 12-13 is less than 0.3 mg / 100 g, and the lecithin content is less than 0.5 mg / 100 g, but the leucine content of Example 14 is greater than 0.3 mg / 100 g, the lecithin content is greater than 0.5 mg / 100 g, and the leucine content and lecithin content of Example 15 are greater than Example 13. This shows that removing the organic solvent from the turbid liquid under a rotary evaporation process at 45-55°C, a vacuum degree of -0.08 to -0.1 MPa, and a rotation speed of 20-100 rpm, and centrifuging at a speed of 1000-10000 rpm can help further improve the effect of the small dog feed additive.

[0092] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A feed additive for small dogs, characterized in that: The invention comprises an additive main material and a coating layer for wrapping the additive main material, wherein the coating layer and the additive main material form a microcapsule with a core-shell structure, and the coating layer comprises the following raw materials: 40-50 parts of lecithin, 4-8 parts of a film former, 4-8 parts of a structure enhancer and 2-4 parts of a natural antioxidant.

2. The small dog feed additive according to claim 1, characterized in that: The structural reinforcing agent is polyethylene glycol or polyvinyl alcohol.

3. The small dog feed additive according to claim 2, characterized in that: The film-forming agent is chitosan or sodium alginate.

4. The small dog feed additive according to claim 1, characterized in that: The natural antioxidant is vitamin E or tea polyphenols.

5. The small dog feed additive according to claim 1, characterized in that: The main additive ingredient includes at least one of amino acids, vitamins and minerals.

6. The small dog feed additive according to claim 5, characterized in that: The main ingredient of the additive includes bile acid.

7. A method for preparing a small dog feed additive according to any one of claims 1 to 6, characterized in that: The steps include: Dissolution: Add the main additive material into the hydration liquid and disperse it evenly to obtain the water phase additive; Hydration: adding lecithin, film former, structure enhancer and natural antioxidant into organic solvent, dispersing evenly, and then mixing evenly with water phase additive to obtain turbid liquid; Purification: Evaporation is performed to remove the organic solvent in the turbid liquid, followed by centrifugation, solid-liquid separation, and drying to obtain a small dog feed additive.

8. The method for preparing a small dog feed additive according to claim 7, characterized in that: The organic solvent is ethanol or ethyl acetate.

9. The method for preparing a small dog feed additive according to claim 7, characterized in that: The hydration liquid is distilled water or physiological saline.

10. The method for preparing a small dog feed additive according to claim 7, characterized in that: In the purification step, the organic solvent in the turbid liquid is removed under a rotary evaporation process at 45-55° C., a vacuum degree of -0.08 to -0.1 MPa, and a rotation speed of 20-100 rpm, and centrifugation is performed at a rotation speed of 1000-10000 rpm, and then the liquid after centrifugation is removed, and the solid after centrifugation is dried to obtain a small dog feed additive.

Images