Herbaceous plant rhizome juice extraction process and extract

Abstract

The invention discloses a process for extracting rhizome juice of herbaceous plants. The method comprises the following steps: selecting raw materials; crushing and mixing; pressing to obtain juice; filtering and removing impurities; primary centrifugal separation; and washing, carrying out secondary centrifugal separation and dialyzing. The method has the beneficial effects that the rhizome is crushed by adopting the crusher and then pressed by adopting the pressing machine, so that the extraction effect of the rhizome juice is improved, the crude liquid is filtered and impurities are removed, in addition, the refined juice is subjected to secondary centrifugal separation, and the supernate is taken, so that the purity of effective components of the rhizome liquid is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of plant extraction, in particular to a process for extracting herb rhizome juice and an extract thereof. Background Art

[0002] Plant essences are high-concentration substances extracted from plants by extraction methods, also known as plant essential oils. Many essences have the functions of stimulating blood circulation, removing toxins and helping different active ingredients to be evenly distributed in the deep layers of the skin, so they are often used to make cosmetics. When extracting plants, the plants are often boiled in boiling water to dissolve the natural ingredients in the plants in water, and then the aqueous solution is purified, but a large amount of nutrients often remain in the plants.

[0003] In the field of plant extraction, it is often necessary to extract the original liquid of the plant first. Plant extraction is to use plants as raw materials, and according to the needs of the final product of the extraction, through the physical and chemical extraction and separation process, to obtain and concentrate one or more active ingredients in the plant in a targeted manner without changing the structure of the active ingredients to form a product;

[0004] 1. For example, a Chinese patent discloses a plant extraction device and method (publication number: CN117818127A), comprising a fixed base and a collecting tank arranged below the fixed base, and an extrusion extraction component is installed above the fixed base. The device is provided with an extrusion extraction component. Through the use of an extrusion bracket, an extrusion grinding roller, an adjustable movable seat, an extrusion motor, a supporting screen plate, a focusing bucket, a driving gear, an adjusting screw block and an adjusting screw, the device can squeeze the plant rhizomes and realize the preliminary extrusion extraction of the internal juice thereof. Through the operation of two sets of extrusion grinding rollers, the plant surface can be squeezed while the plant rhizomes themselves can be driven to move relative to each other, so that the plant rhizomes can be fully squeezed, the crushing effect can be improved, and the waste of plant stock solution can be avoided. Not only the extraction efficiency and effect of the plant stock solution are improved, but also the subsequent collection of the extracted residues is conducive to meet people's use needs.

[0005] 2. A process for preparing a natural plant extract essence (publication number: CN113599308A), which includes the following steps: Step 1: prepare fresh plants, retain the roots, stems and leaves, and cut them into small pieces of equal length; Step 2: squeeze the cut plant materials to initially squeeze out the juice; Step 3: add the squeezed plant debris to the solution to dilute and extract the juice inside the plant; Step 4: collect the two juices and heat and stir them together. It has the advantage of being able to crush the plants and extract the juice.

[0006] Although the existing extraction devices in daily life have met the users' needs to a certain extent, there are still certain defects in the use process. The specific problems are as follows: at present, in the process of extracting plant stock solution, a blade is usually used to break the plant rhizomes for stock solution extraction. Since the plant rhizomes are relatively tough, the juice inside them is not easy to penetrate out, resulting in poor extraction effect. Most of the stock solution in the plant cannot be fully extracted, which easily causes waste of plant stock solution. At the same time, the extraction efficiency of plant stock solution is low and cannot meet people's use needs.

[0007] In view of the above problems, it is necessary to propose a herbal rhizome juice extraction process and extract. Summary of the invention

[0008] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a herb rhizome juice extraction process and extract to solve the above-mentioned problems.

[0009] A process for extracting juice from herb rhizomes, the method steps are as follows:

[0010] S1. Select raw materials;

[0011] S2, crushing and mixing;

[0012] S3, pressing to extract juice;

[0013] S4, filtering and removing impurities;

[0014] S5, one centrifugal separation;

[0015] S6, washing, secondary centrifugation, and dialysis.

[0016] The raw material selected in step S1 is to wash the plant rhizomes and remove the rotten parts.

[0017] The crushing in step S2 is to put the plant rhizomes into a crusher for crushing to produce rhizome powder.

[0018] In step S3, the rhizome powder is put into a pressing machine to press out the juice into a coarse liquid.

[0019] Step S4 is to filter the crude liquid to remove impurities and obtain refined juice.

[0020] Step S5 is to put the refined juice into a centrifuge for centrifugal separation and take the supernatant.

[0021] In step S6, the supernatant is washed with deionized water, then placed in a centrifuge for secondary centrifugation, dialyzed to obtain the supernatant, and the rhizome extract is obtained.

[0022] The crude liquid is filtered by multi-stage filter cloth, and the multi-stage filter cloth is made of non-woven fabric.

[0023] A herb rhizome extract prepared by the process according to any one of claims 1 to 8.

[0024] Compared with the prior art, the present invention has the following beneficial effects: the present invention adopts a crusher to crush the rhizomes, and then adopts a pressing machine to press them, so as to improve the extraction effect of rhizome juice, filter the crude liquid to remove impurities, and further perform secondary centrifugal separation on the refined juice to take the supernatant, so as to improve the purity of the effective components of the rhizome liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The figure is a schematic diagram of the process for extracting juice from herbal rhizomes of the present invention. DETAILED DESCRIPTION

[0026] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0027] The embodiments of the present invention are described in detail below with reference to the accompanying drawings, but the present invention can be implemented in many different ways as defined and covered by the claims.

[0028] like Figure 1 As shown, a process for extracting juice from herbal rhizomes comprises the following steps:

[0029] S1. Select raw materials;

[0030] S2, crushing and mixing;

[0031] S3, pressing to extract juice;

[0032] S4, filtering and removing impurities;

[0033] S5, one centrifugal separation;

[0034] S6, washing, secondary centrifugation, and dialysis.

[0035] The raw material selected in step S1 is to wash the plant rhizomes and remove the rotten parts.

[0036] The crushing in step S2 is to put the plant rhizomes into a crusher for crushing to produce rhizome powder.

[0037] In step S3, the rhizome powder is put into a pressing machine to press out the juice into a coarse liquid.

[0038] Step S4 is to filter the crude liquid to remove impurities and obtain refined juice.

[0039] Step S5 is to put the refined juice into a centrifuge for centrifugal separation and take the supernatant.

[0040] In step S6, the supernatant is washed with deionized water, then placed in a centrifuge for secondary centrifugation, dialyzed to obtain the supernatant, and the rhizome extract is obtained.

[0041] The crude liquid is filtered by multi-stage filter cloth, and the multi-stage filter cloth is made of non-woven fabric.

[0042] A herb rhizome extract prepared by the process according to any one of claims 1 to 8.

[0043] Compared with the prior art, the present invention has the following beneficial effects: the present invention adopts a crusher to crush the rhizomes, and then adopts a pressing machine to press them, so as to improve the extraction effect of rhizome juice, filter the crude liquid to remove impurities, and further perform secondary centrifugal separation on the refined juice to take the supernatant, so as to improve the purity of the effective components of the rhizome liquid.

[0044] The plant rhizomes include Cyperus rotundus rhizomes and Black Cimicifuga rhizomes; the results of the antibacterial activity of the methanol crude extract of Cyperus rotundus rhizomes on 9 tested plant pathogenic fungi showed, as shown in Table 1: it has a certain degree of mycelial growth inhibitory activity on the tested plant pathogenic fungi, and the inhibitory effect on Saccharomyces solani at a concentration of 10 mg / mL is the most significant, with an inhibition rate of 100%; the inhibitory effect on Fusarium graminearum and Sugarcane pineapple pathogen is second, with inhibition rates of 81.84% and 81.21% respectively; the inhibitory effect on Rhizoctonia solani, Sugarcane tip rot and Banana coal leaf rot is the worst, with inhibition rates between 43.79% and 46.07%.

[0045] At a concentration of 5 mg / mL, the inhibition rates of petroleum ether extracts on the 9 tested plant pathogenic fungi were all above 50.00%, among which the inhibition effect on Fusarium graminearum, Sclerotium solani and Pathogenis pyrifos was the most significant, with an inhibition rate of more than 85%, and the inhibition effect on banana soya leaf spot was the worst, with an inhibition rate of only 50.00%; the inhibition rates of ethyl acetate extracts on Fusarium graminearum, Fusarium wilt of bitter melon, Sclerotium solani, Pathogenis pyrifos of sugarcane and Alternaria napus were all above 90%, and the inhibition effect on Rhizoctonia solani and Pathogenis pyrifos of sugarcane was the worst, with an inhibition rate of less than 60%. The n-butanol extract only had antibacterial activity against three pathogens, namely, Pitaya canker, Rhizoctonia solani and Pathogenis pyrifos of sugarcane, and the inhibition rate was only about 50%. The water extract had no inhibitory effect on the tested plant pathogenic fungi. The results of biological activity assay indicated that the antibacterial active ingredients in the rhizomes of Cyperus rotundus might be mainly distributed in the petroleum ether and ethyl acetate extracts.

[0046] Determination of the antibacterial activity of the extracts from the rhizomes of Cyperus rotundus The mycelial growth rate method was used to determine the inhibitory effects of the methanol crude extract of the rhizomes of Cyperus rotundus and each extract on 9 plant pathogenic fungi. Weigh a certain amount (1 g of crude methanol extract and 0.5 g of extract) of the extract to be tested, add a certain amount of methanol (methanol content not exceeding 2%), fully dissolve, add sterile water to prepare a mother solution of the target concentration (1000 mg / mL for crude methanol extract and 500 mg / mL for extract), take 1 mL and mix with 99 mL of PDA medium, mix thoroughly, pour into a culture dish with a diameter of 7.5 cm, obtain a test drug-containing culture medium, place it in an ultra-clean workbench to cool, use a strain puncher to punch out a 6 mm diameter bacterial cake from the activated strain culture medium, place the bacterial cake upside down in the center of the culture medium, and culture it in a constant temperature incubator at 28°C. Repeat each treatment 3 times, and use the treatment containing an equal amount of methanol as the control group. When the colony diameter of the control group reaches 5 cm or more, the colony diameter is measured by the cross method, and the antibacterial rate is calculated according to the following formula.

[0047] Antibacterial rate (%) = (D1-D2) / (D1-6) × 100

[0048] Where D1 represents the colony diameter of the control group, and D2 represents the colony diameter of the treatment group (unit: cm).

[0049] The toxicity of the extracts of Cyperus rotundus rhizomes on the mycelial growth of pathogenic fungi was determined. The mycelial growth rate method was used to determine the toxicity of the extracts with an inhibition rate of more than 70% on the mycelial growth of the tested plant pathogenic fungi. The extracts to be tested were dissolved in a certain amount of methanol, diluted with sterile water to a mother solution with a concentration of 500 mg / mL, and then a series of mother solutions with concentration gradients were prepared by a half-fold dilution method. In the clean bench, 1 mL of the mother solution of the series of concentration gradients was mixed with 99 mL of melted PDA culture medium, and a series of drug-containing culture media with concentrations of 5 mg / mL, 2.5 mg / mL, 1.25 mg / mL, 0.625 mg / mL and 0.3125 mg / mL were prepared, respectively, to calculate the EC50 of each extract of Cyperus rotundus rhizomes against pathogenic fungi.

[0050] Table 1 Inhibitory activity of extracts from the rhizomes of Cyperus rotundus against mycelial growth of nine plant pathogenic fungi

[0051]

[0052] The experimental data were statistically analyzed using Excel 2019 and SPSS software, and the variance analysis was performed using one-way analysis of variance (ANOVA) and Duncan's new multiple range method.

[0053] Black cohosh rhizome extract can not only achieve the effect of treating menopausal symptoms of breast cancer patients by regulating the levels of various hormones in the body; at the same time, it can increase the level of high-density lipoprotein (HDL) and reduce the level of low-density lipoprotein (LDL), thereby achieving the purpose of preventing coronary heart disease and reducing the risk of cardiovascular and cerebrovascular diseases in menopausal women. In the human skeletal system, black cohosh rhizome extract produces estrogen-like effects through ER mediation and activation of ER, enhances the activity of alkaline phosphatase in bone, increases the ratio of osteoprotegerin to osteoclast activation factor, and then enhances the activity of osteoblasts, accelerates the rate of bone synthesis, reduces bone destruction, and thus significantly increases the bone density of menopausal osteoporosis patients, achieving the purpose of treating osteoporosis in menopausal patients. With the continuous exploration of the mechanism of black cohosh rhizome extract in treating menopausal symptoms, the efficacy of this drug in treating menopausal symptoms in breast cancer patients has become more and more certain. Pure plant preparations-black cohosh rhizome extract will surely become the development direction of treating menopausal symptoms in breast cancer patients.

[0054] The mechanism of action of black cohosh rhizome extract may be to regulate endocrine balance by inhibiting the levels of follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the body. Black cohosh rhizome extract can directly act on the central nervous system and is a competitive ligand for 5-hydroxytryptamine (5-HT). The 5-HT system plays an important role in the occurrence of hot flashes. Some scholars also believe that black cohosh rhizome extract can enhance the chloride ion current intensity of γ-aminobutyric acid type A receptor (GABA), thereby regulating the release of GABA, which can effectively relieve menopausal symptoms and autonomic dysfunction symptoms. Reame et al. used naloxone blockade and positron imaging technology to prove that black cohosh rhizome extract has endogenous opioid activity.

[0055] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A process for extracting juice from herb rhizomes, characterized in that: The method steps are: S1. Select raw materials; S2, crushing and mixing; S3, pressing to extract juice; S4, filtering and removing impurities; S5, one centrifugal separation; S6, washing, secondary centrifugation, and dialysis.

2. The herb rhizome juice extraction process according to claim 1, characterized in that: The raw material selected in step S1 is to wash the plant rhizomes and remove the rotten parts.

3. The herb rhizome juice extraction process according to claim 1, characterized in that: The crushing in step S2 is to put the plant rhizomes into a crusher for crushing to produce rhizome powder.

4. The process for extracting herb rhizome juice according to claim 1, characterized in that: In step S3, the rhizome powder is put into a pressing machine to press out the juice into a coarse liquid.

5. The process for extracting herb rhizome juice according to claim 1, characterized in that: Step S4 is to filter the crude liquid to remove impurities and obtain refined juice.

6. The process for extracting herb rhizome juice according to claim 1, characterized in that: Step S5 is to put the refined juice into a centrifuge for centrifugal separation and take the supernatant.

7. The process for extracting herb rhizome juice according to claim 1, characterized in that: In step S6, the supernatant is washed with deionized water, then placed in a centrifuge for secondary centrifugation, dialyzed to obtain the supernatant, and the rhizome extract is obtained.

8. The process for extracting herb rhizome juice according to claim 1, characterized in that: The crude liquid is filtered by multi-stage filter cloth, and the multi-stage filter cloth is made of non-woven fabric.

9. A herbal rhizome extract, characterized in that: The method is prepared by the process as described in any one of claims 1 to 8.

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