Method and system for improving grafting survival rate of Yunnan carefree

Abstract

The invention belongs to the technical field of Yunnan carefree flower breeding, and particularly relates to a method and system for improving the grafting survival rate of Yunnan carefree flower. Stock selection; scion selection; collecting and processing scions; preparing a wound healing promoting agent; combining the scion with the rootstock; after grafting is completed, wet moss is evenly laid on the soil layer of the nutrition bag, and the moisturizing effect is achieved. Compared with other propagation methods, the method has the advantages that operation is easy, the propagation amount is large, grafted trees grow fast, and flowering is advanced.

Description

Technical Field

[0001] The present invention belongs to but is not limited to the field of Yunnan Worry-Free Flower breeding technology, and in particular relates to a method and system for improving the survival rate of Yunnan Worry-Free Flower grafting Background Art

[0002] Yunnan Worry-Free Flower is a woody plant belonging to the Leguminosae family. It has huge flowers and good landscape effects. However, due to its growth habits and environmental requirements, its grafting survival rate is not ideal, which greatly limits its reproduction and variety improvement process.

[0003] Grafting, as one of the artificial vegetative propagation methods of plants, is to graft the branch or bud of one plant (i.e. scion) onto the stem or root of another plant (i.e. stock), using the healing function of the plant after injury, so that the cambium of the two wound surfaces are close and tied together, and finally grow into a complete plant. However, due to the influence of many factors such as the affinity of the stock and scion, nutritional status, water, pollution, hormone level, shading, etc. during the grafting process, the healing speed and survival rate of the grafting interface are often difficult to guarantee.

[0004] In response to the problem of low survival rate of Yunnan Worry-Free Flower grafting, researchers have been exploring effective solutions. Among them, the use of healing agents is an important means to improve the survival rate of grafting. The healing agent can form a protective film at the grafting site to prevent the loss of water and nutrients, while promoting the formation and growth of callus tissue, thereby improving the survival rate of grafting. However, there are many types of healing agents on the market, but special healing agents for Yunnan Worry-Free Flower grafting are still relatively rare. At the same time, due to the special growth habits of Yunnan Worry-Free Flower and its requirements for environmental conditions, the use of improper healing agents or methods of use will lead to grafting failure or low survival rate. Summary of the invention

[0005] In view of the problems existing in the prior art, the present invention provides a method for improving the grafting survival rate of Yunnan Wortilla yunnanensis.

[0006] The present invention is achieved by a method for improving the survival rate of Yunnan Worry-Free Flower grafting, the method comprising:

[0007] S1: Tool preparation: pruning shears, grafting knife, plastic film, etc.

[0008] S2: Rootstock selection: Choose a two-year-old, 30cm tall, disease-free, and healthy Chinese hollyhock. Use pruning shears to make a flat cut 20cm above the soil layer of the nutrient bag, keeping the cut smooth.

[0009] S3: Selection of scion: Select the mother tree for scion, which is healthy, free of pests and diseases, and blooms luxuriantly;

[0010] S4: Collection and treatment of scions: Grafting scions should be collected before the end of February. The collected branches should be soaked in carbendazim solution for sterilization and disinfection, and 2 / 3 of the leaves on the branches should be cut off to reduce water evaporation; when grafting, they should be cut into stem segments with 1-2 buds, and all leaves and petioles above the base of the petiole should be cut off to accelerate the complete and natural shedding of the petiole without damaging the axillary buds. In addition, in order to promote the differentiation of axillary buds, the top buds of the stem segments should be removed;

[0011] S5: Preparation of wound healing promoting agent:

[0012] 30mg / L6-BA+40mg / LNAA+200mg / L octahydroxyquinoline sulfate+sticky yam mucus (acting as a water-retaining agent) 1L;

[0013] S6: scion and rootstock combined;

[0014] The scion obtained in S4 is tightly attached to the inner side of the cut surface of the stock by cleft grafting, the wound healing promoting agent obtained in S5 is applied to the wound attachment area, and the entire scion and wound are tightly sealed with a plastic film;

[0015] S7: After grafting, spread damp moss evenly on top of the soil layer in the nutrient bag to keep it moist.

[0016] Furthermore, the healing agent in S4 is generally required to be prepared and used immediately. When used, the healing agent is brushed on the grafting wound with a soft brush to form a water-retaining layer.

[0017] Furthermore, the preparation method of the water retaining agent in the wound healing promoting agent in S5 is: using a sharp knife to cut the cross section of the sticky yam tuber, using a scraper to scrape the mucus from the fresh section, and storing it in a brown light-proof bottle.

[0018] Furthermore, the thickness of the plastic film in S6 is 0.06 mm.

[0019] Further, the method of using the healing agent is as follows:

[0020] Add 30mg / L6-BA+40mg / LNAA+200mg / L octahydroxyquinoline sulfate to the collected mucus, use the concentration of the water retaining agent as indicated on the package, and apply it to the wound.

[0021] The present invention also provides a system for improving the survival rate of Yunnan Worry-Free Flower grafting, the system comprising:

[0022] Tool preparation module, used to prepare tools such as pruning shears, grafting knives, plastic film, etc.

[0023] The rootstock selection module is used to select a biennial, moderately tall, pest-free, and robust Chinese hollyhock, cut off the upper part 15-20 cm away from the soil layer of the nutrient bag to form a flat incision, and split it at the incision to form a V-shaped incision;

[0024] The scion selection module is used to select scions from excellent individual plants that are healthy, free of pests and diseases, and bloom luxuriantly;

[0025] The scion processing module is used to sterilize and disinfect the collected scions, and perform leaf and stem cutting operations;

[0026] A wound healing agent preparation module, used to prepare a wound healing agent with a specific ratio;

[0027] The scion and stock combination module is used to fit the treated scion tightly to the inner side of the cut surface of the stock and bandage it with wound healing agent and plastic film;

[0028] Moisturizing module for moisturizing with damp moss after grafting is completed.

[0029] The healing agent processing module further comprises:

[0030] A ready-to-use unit to ensure immediate preparation and immediate use of the healing agent;

[0031] The brushing unit is used for brushing the healing agent on the grafted wound joint to form a water-retaining layer.

[0032] The wound healing agent preparation module further includes:

[0033] The water retaining agent preparation unit is used for scraping the mucus from the fresh cross section of the sticky yam tuber and storing it in a brown light-proof bottle for use as a water retaining agent.

[0034] The plastic film used in the scion and stock combination module has a specific thickness of 0.06 mm to ensure good sealing and air permeability, thereby improving the survival rate of the graft.

[0035] In combination with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions to be protected by the present invention are as follows:

[0036] First, the present invention can improve the grafting survival rate. Yunnan Worry-Free Flower is a highly lignified woody plant. The use of the self-prepared wound healing agent can prevent water loss, accelerate wound healing, and improve the survival rate of the scion, which can reach more than 90%.

[0037] The invention can maintain the excellent characteristics of the scion variety. The grafting method can maintain the excellent characteristics of the old stem of the mother plant producing flowers with a large amount of flowers and bright colors.

[0038] The invention can shorten the flowering time of Yunnan Cassia truncatula. Compared with traditional seed propagation, the grafting method can effectively shorten the flowering time.

[0039] The method is simple to operate and has a large reproduction amount. The fruiting rate of Yunnan Worry-Free Flower is low and the seed reproduction amount is small. The method of the present invention is simple and feasible to operate, has a large number of buds and a large reproduction amount.

[0040] Second, as auxiliary evidence of the inventiveness of the claims of the present invention, it is also reflected in the following important aspects:

[0041] (1) The expected benefits and commercial value of the technical solution of the present invention after transformation are as follows: Yunnan Worry-Free Flower belongs to the genus Worry-Free Flower of the Leguminosae family. It is distributed in Myanmar and Yingjiang, Yunnan, China. It grows in areas with an altitude of 300 to 1,200 meters. It is found in high mountain dense forests or sparse forests and has not yet been artificially introduced for cultivation. Because its seeds are easily eaten by birds and beasts, and its seed fruiting rate is low, the distribution range of Yunnan Worry-Free Flower population is extremely narrow. In the "Red List of the World Conservation Union" (IUCN), its protection level is endangered. Yunnan Worry-Free Flower has the characteristics of old stems blooming, huge flowers, bright colors, and a long flowering period. It is a good landscape tree species. This technology solves the problems of the difficulty of Worry-Free Flower seed propagation and the low grafting survival rate through grafting, and also solves the problem of the long flowering cycle of seedlings. Through market research, after the technical solution is transformed, the average market price of annual grafted seedlings can be 300-500 yuan / plant.

[0042] (2) The technical solution of the present invention fills the technical gap in the industry at home and abroad: because there is basically no relevant research on Yunnan Worry-Free Flower at present, preliminary experiments have found that the survival rate of ordinary grafting methods is low. The invention technology can greatly improve the survival rate of Yunnan Worry-Free Flower grafting, fundamentally solving the problem of Yunnan Worry-Free Flower reproduction.

[0043] Third, the technical solution of the present invention improves some key problems in the prior art, which are specifically reflected in the following aspects:

[0044] Problems with existing technologies:

[0045] 1) Low grafting survival rate: In traditional grafting methods, the survival rate is often not high, which affects the production efficiency of high-quality seedlings.

[0046] 2) Spread of pests and diseases: During the grafting process, plants are easily attacked by pests and diseases, which may lead to grafting failure or damage to plant health.

[0047] 3) Water evaporates too quickly: Improper handling of the scion causes water to evaporate too quickly, affecting the survival rate of the scion.

[0048] 4) Poor wound healing: Lack of effective measures to promote wound healing results in loose bonding between the scion and the rootstock and low survival rate.

[0049] 5) Complex grafting technology: The grafting operation in the existing technology is complicated and difficult to standardize and promote.

[0050] Improvements and significant advances in technical solutions:

[0051] 1) Improve the survival rate of grafting:

[0052] Step optimization: Through detailed step optimization, including tool preparation, rootstock and scion selection, scion treatment, etc., the standardization and survival rate of grafting operations are improved.

[0053] Cleft grafting: The cleft grafting method is used to closely fit the cut surface of the scion and the rootstock, ensuring a tight connection between the grafted parts and improving the survival rate.

[0054] 2) Effective pest and disease control:

[0055] Disinfection: After the scion is collected, it is soaked in a solution of carbendazim for sterilization and disinfection, which effectively prevents the spread of diseases and pests and ensures the health of the scion.

[0056] 3) Reduce water evaporation:

[0057] Leaf treatment: When handling the grafted scion, cut off 2 / 3 of the leaves to reduce water evaporation, maintain the moisture of the scion, and improve the survival rate.

[0058] 4) Promote wound healing:

[0059] Wound healing agent: Prepare and use a wound healing agent containing 30 mg / L 6-BA, 40 mg / L NAA, 200 mg / L octahydroxyquinoline sulfate and sticky yam mucus, apply it to the wound adhesion area to accelerate healing and ensure a close bond between the scion and the rootstock.

[0060] 5) Easy to operate:

[0061] Standardized process: Detailed steps and tool preparation are provided to simplify the operation process, making the grafting technology more standardized and easier to promote and apply.

[0062] Significant technological advancements:

[0063] 1) The survival rate is greatly improved: By optimizing the grafting methods and steps, the grafting survival rate of Yunnan Worry-Free Flower is improved, and the production efficiency of high-quality seedlings is significantly improved.

[0064] 2) Healthy plants: Effective pest and disease control measures ensure the health of scions and rootstocks and reduce the risk of pest and disease transmission.

[0065] 3) Water management: Through reasonable leaf treatment and the use of moisturizing materials, the moisture of the scion is ensured and the survival rate is improved.

[0066] 4) Rapid healing: The use of wound healing agents can speed up the healing of scion and rootstock and ensure the survival rate of grafting.

[0067] 5) Easy to promote: The simplified operation process and standardized steps make this method easy to promote and apply, and it has wide practical value.

[0068] In summary, the present invention provides a method for improving the survival rate of Yunnan Worry-Free Flower grafting by improving the key problems in the prior art, which has made significant progress in grafting technology and has important application prospects and promotion value.

[0069] Fourth, the present invention provides a method for improving the survival rate of Yunnan worry-free flower grafting, which significantly improves the success rate of grafting by optimizing the grafting process and using a wound healing agent. Specific parameters include selecting a two-year-old, 30 cm, disease-free and insect-free robust rootstock, using a carbendazim solution to disinfect the collected scion, and cutting off 2 / 3 of the scion leaves to reduce water evaporation. In addition, the survival rate of grafting is further improved by closely fitting the scion and the rootstock using a cleft grafting method, treating the wound with a special wound healing agent, and then wrapping it with a plastic film.

[0070] This technology effectively solves the problem of low graft survival rate. Through scientific treatment and sterilization of scions, the risk of disease and pest transmission is reduced. Reasonable leaf treatment and moss covering measures help to maintain the moisture of scions and prevent water from evaporating too quickly. In addition, the specially formulated wound healing agent accelerates the wound healing process and ensures the close combination of scions and rootstocks, thereby improving the success rate of grafting and the formation of healthy plants.

[0071] Compared with the prior art, the present invention significantly improves the grafting survival rate, simplifies the operation steps, and makes the grafting process more standardized and easy to promote. Through a scientific grafting method and detailed operation steps, the present invention provides an efficient, simple and reliable grafting method, which has important application value and promotion prospects. At the same time, effective pest and disease control and water management measures ensure the healthy growth of grafted plants, further improving the success rate of grafting.

[0072] In summary, the present invention has made significant technical progress in the field of grafting technology and solved the key problems in the prior art. Through scientific technical parameters and improved methods, the present invention provides an efficient and easy-to-operate grafting method, which provides important technical support for the production and promotion of Yunnan Worry-Free Flower, and is of great significance to ecological environment protection and efficient resource utilization.

[0073] Fifth, the technical effects brought by the parameters of the present invention:

[0074] 1. Improve the grafting survival rate: By selecting high-quality rootstocks and scions and adopting appropriate grafting methods (such as cleft grafting), the grafting success rate of Yunnan Worry-Free Flower can be effectively improved. In particular, the selection of rootstocks and the processing steps of scions ensure a good combination of the grafting site, thereby increasing the grafting survival rate.

[0075] 2. Promote wound healing: Using a specific formula of wound healing agents (including 6-BA, KT, NAA, octahydroxyquinoline sulfate and sticky yam mucus) can effectively promote wound healing. The plant growth regulators and water retaining agents in this formula can accelerate the wound healing process, reduce the risk of infection, and increase the possibility of successful grafting.

[0076] 3. Reduce water evaporation: By cutting off 2 / 3 of the leaves of the grafted scion and wrapping the grafted area with plastic film, water evaporation can be significantly reduced, thereby reducing the water loss rate of the scion, maintaining its activity during the grafting process, and further improving the success rate of grafting.

[0077] 4. Increase the humidity of the grafting site: After the grafting is completed, use moist moss to spread on top of the soil layer of the nutrient bag to help maintain the humidity of the grafting site. This measure can effectively reduce water loss, keep the grafting site moist, and is conducive to the healing and growth of the scion and rootstock.

[0078] 5. Optimize scion processing: Sterilize the collected scions and cut off the terminal buds and unnecessary leaves. This will help prevent bacterial infection, reduce water evaporation, promote the normal development of axillary buds, and further improve the success rate of grafting.

[0079] In summary, the present invention significantly improves the grafting survival rate and survival quality of Eupatorium yunnanensis through systematic grafting steps and precise parameter settings, and has good technical effects and practical application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0080] Figure 1 The invention discloses a flow chart of a method for improving the survival rate of grafting of Yunnan Worry-Free Flower provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0081] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0082] Embodiment 1

[0083] Implementation background: In order to promote the excellent varieties of Yunnan Worry-Free Flower, an agricultural research unit decided to adopt efficient grafting technology to improve the grafting survival rate, especially to conduct large-scale grafting experiments in early spring.

[0084] Implementation steps:

[0085] 1) Tool preparation: prepare sharp pruning shears, several special grafting knives, alcohol cotton balls for disinfection, sufficient plastic film and binding tape.

[0086] 2) Rootstock selection: Select 30 biennial Chinese hollyhocks from the nursery that are about 30 cm tall, disease-free, and healthy as rootstocks. Use pruning shears to make a smooth cut 15-20 cm above the soil layer of the nutrient bag, ensuring that the cut is flat and without tearing.

[0087] 3) Selection of scion: From the identified excellent Yunnan Wormwood mother trees, select several branches that are vigorously growing, free of pests and diseases, and blooming luxuriantly as the source of scion.

[0088] 4) Collection and treatment of scion: In mid-February, the collected scion branches were immediately soaked in carbendazim solution (concentration is 1000 times the recommended dose) at the base of the branches for sterilization and disinfection. After treatment, about 2 / 3 of the leaves on the branches were cut off, leaving a small amount of leaves for observing the state of the scion. Then, the branches were cut into stem segments with 1-2 full buds each, ensuring that the leaves and petioles near the base of the petioles on each stem segment were completely removed, and the terminal buds were also removed to promote the differentiation of lateral buds.

[0089] 5) Preparation of wound healing promoting agent: According to the formula (30 mg / L 6-BA+40 mg / L NAA+200 mg / L octahydroxyquinoline sulfate+1 L sticky yam mucus), accurately weigh and mix the components, stir evenly and set aside.

[0090] 6) Scion and stock combination: Use the cleft grafting method to fit the treated scion tightly to the inside of the cut of the stock, ensuring that the cambium is aligned. Then, evenly apply the wound healing agent prepared in step 5 to the joint, and wrap it tightly with plastic film to ensure a good seal.

[0091] 7) Moisturizing measures: After the grafting is completed, immediately spread a layer of moist moss evenly on top of the soil layer of the nutrient bag to maintain the environmental humidity, which is conducive to the healing of the grafting wound.

[0092] Embodiment 2

[0093] Implementation background: In order to improve the planting quality of Yunnan Worry-Free Flower, a flower base decided to conduct a grafting experiment in early spring to screen out the best varieties that are most suitable for local climatic conditions.

[0094] Implementation steps (similar to Example 1, but slightly different in details):

[0095] 1) Tool preparation: In addition to conventional tools, a thermometer and hygrometer are also prepared to monitor the grafting environment.

[0096] 2) Rootstock selection: 50 two-year-old Chinese worry-free flowers that met the standards were selected from the base as rootstocks, with special attention paid to selecting plants with well-developed root systems and uniform growth.

[0097] 3) Selection and collection of scions: Nutrition management of candidate mother trees is carried out one month in advance to ensure the quality of scions. Before the end of February, scions are collected from mother trees in the best condition, and the processing method is the same as that in Example 1.

[0098] 4) Fine-tuning of wound healing agents: Taking into account the specific environmental conditions of the base, the concentration of 6-BA in the wound healing agent was fine-tuned to 25 mg / L, and the other ingredients remained unchanged to better adapt to the local climate.

[0099] 5) Combination of scion and rootstock: During the grafting process, special emphasis is placed on operation speed to ensure that the connection between scion and rootstock is completed in a short time and reduce exposure time.

[0100] 6) Moisture retention and monitoring: After grafting, not only moist moss was laid, but also a timed spray system was set up to automatically maintain the environmental humidity. At the same time, a thermometer and hygrometer were used to record data daily and adjust management measures in a timely manner.

[0101] 7) Subsequent management: Within two weeks after grafting, strengthen the observation of the grafted seedlings, remove the suckers on the rootstock in time, and ensure that nutrients are concentratedly supplied to the scion.

[0102] like Figure 1 As shown, an embodiment of the present invention provides a method for improving the survival rate of Yunnan Worry-Free Flower grafting, the method comprising:

[0103] S1: Tool preparation: pruning shears, grafting knife, plastic film, etc.

[0104] S2: Rootstock selection: Choose a two-year-old, 30cm tall, disease-free, and healthy Chinese hollyhock. Use pruning shears to make a flat cut 20cm above the soil layer of the nutrient bag, keeping the cut smooth.

[0105] S3: Selection of scion: Select the mother tree for scion, which is healthy, free of pests and diseases, and blooms luxuriantly;

[0106] S4: Collection and treatment of scions: Grafting scions should be collected before the end of February. The collected branches should be soaked in carbendazim solution for sterilization and disinfection, and 2 / 3 of the leaves on the branches should be cut off to reduce water evaporation; when grafting, they should be cut into stem segments with 1-2 buds, and all leaves and petioles above the base of the petiole should be cut off to accelerate the complete and natural shedding of the petiole without damaging the axillary buds. In addition, in order to promote the differentiation of axillary buds, the top buds of the stem segments should be removed;

[0107] S5: Preparation of wound healing promoting agent:

[0108] 30mg / L6-BA+40mg / LNAA+200mg / L octahydroxyquinoline sulfate+mucilage extracted from yam tuber (acting as a water retaining agent) 1L;

[0109] S6: scion and rootstock combined;

[0110] The scion obtained in S4 is tightly attached to the inner side of the cut surface of the stock by cleft grafting, the wound healing promoting agent obtained in S5 is applied to the wound attachment area, and the entire scion and wound are tightly sealed with a plastic film;

[0111] S7: After grafting, spread damp moss evenly on top of the soil layer in the nutrient bag to keep it moist.

[0112] The healing agent in S4 is generally required to be prepared and used immediately. When used, the healing agent is brushed on the grafting wound with a soft brush to form a water-retaining layer.

[0113] The preparation method of the water retaining agent in the wound healing promoting agent in S5 is: cut the cross section of the sticky yam tuber with a sharp knife, scrape the mucus from the fresh section with a scraper, and store it in a brown light-proof bottle.

[0114] The thickness of the plastic film in S6 is 0.06 mm.

[0115] The method of using the healing agent is as follows:

[0116] Add 30mg / L6-BA+40mg / LNAA+200mg / L octahydroxyquinoline sulfate to the collected mucus, use the concentration of the water retaining agent as indicated on the package, and apply it to the wound.

[0117] The present invention can be specifically applied in the field of seedling propagation of Yunnan Worry-free Flower.

[0118] Screening of healing agents suitable for grafting of Yunnan Silene scabra

[0119] 1.1 Experimental materials: Yunnan Sophora japonica as scion and Chinese Sophora japonica as rootstock

[0120] Experimental reagents: The protective agent is octahydroxyquinoline sulfate, the signal regulators are kinetin (KT), cytokinin (6-BA), naphthylacetic acid (NAA), indolebutyric acid (IBA), 2,4-dichlorophenoxyacetic acid (2,4-D), the water-retaining agent is sticky yam mucus, and CK is clear water control.

[0121] 1.2 Experimental methods

[0122] (1) Tool preparation: pruning shears, grafting knife, plastic film, etc.

[0123] (2) Rootstock selection: Choose a two-year-old Chinese hollyhock that is about 30 cm tall, free of pests and diseases, and strong in growth. Use pruning shears to make a flat incision 20 cm above the soil layer of the nutrient bag, keeping the incision smooth.

[0124] (3) Selection of scion: The mother tree for scion selection should be a strong, disease-free, insect-free, and luxuriantly flowering high-quality single plant.

[0125] (4) Collection and treatment of scions: Grafting scions should be collected before the end of February. The collected branches should be soaked in 800 times carbendazim solution for sterilization and disinfection, and 2 / 3 of the leaves on the branches should be cut off to reduce water evaporation. When grafting, the stems should be cut into segments with 1-2 buds. All leaves and petioles above the base of the petiole should be cut off to accelerate the complete and natural shedding of the petiole without damaging the axillary buds. In addition, in order to promote the differentiation of axillary buds, the top buds of the stem segments should be removed.

[0126] (5) Preparation and use of healing agent: Use a sharp knife to cut the cross section of the tuber of Dioscorea opposita (a twining herbaceous vine of the Dioscorea genus of the Dioscorea family), scrape the mucus from the fresh section with a scraper, store it in a brown light-proof bottle, and add 30 mg / L 6-BA + 40 mg / L NAA + 200 mg / L octahydroxyquinoline sulfate to the collected mucus. The protective agent uses the concentration indicated on the package, and the method of use is to apply it to the wound.

[0127] (6) Grafting method

[0128] Use the cleft grafting method to fit the scion and the inside of the cut surface of the rootstock tightly, use a soft brush to apply the wound healing agent to the wound joint, and use a 0.06mm thick plastic film to seal the entire scion and wound. After the grafting is completed, spread the damp moss evenly on the soil layer of the nutrient bag to play a moisturizing role.

[0129] 1.3 Experimental Results and Analysis

[0130] 1.3.1 Screening of optimal concentration of signal modulators

[0131] As shown in Table 1, the five signal regulators improved the grafting survival rate compared with CK, among which NAA, 6-BA and KT had the best effect. Through the screening of the optimal concentration, the optimal concentration of NAA was 40 mg / L, and its grafting survival rate reached 92%; the optimal concentration of KT was 0.2 mg / L, and its grafting survival rate reached 90%; the optimal concentration of 6-BA was 30 mg / L, and its grafting survival rate reached 92%.

[0132] Table 1 Graft survival rate after treatment with different signal modulators

[0133]

[0134] 1.3.2 Screening of optimal concentrations of different signal modulators

[0135] Three regulators with higher grafting survival rates for Yunnan Wormwood were selected, 40mg / L NAA, 0.2mg / L KT, and 30mg / L 6-BA, and the grafting survival rate was investigated. The results showed (Table 2) that the 30mg / L 6-BA + 40mg / L NAA compound solution had the best effect when applied to the wound closure, and its grafting survival rate reached 94%.

[0136] Table 2 Screening of optimal concentrations of different regulators

[0137]

[0138]

[0139] The present invention uses a healing agent formed by compounding a regulator (30 mg / L 6-BA, 40 mg / L NAA), a protective agent (200 mg / L octahydroxyquinoline sulfate), and a water retaining agent (sticky yam mucus) and smearing it on the grafting wound joint, which can effectively improve the grafting survival rate of Yunnan worry-free flower.

[0140] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with the technical field within the technical scope disclosed by the present invention and within the spirit and principle of the present invention should be covered by the protection scope of the present invention.

Claims

1. A method for improving the survival rate of Yunnan Worry-Free Flower grafting, characterized in that: The method includes: S1: Tool preparation: pruning shears, grafting knife, plastic film; S2: Rootstock selection: Choose a two-year-old, 30 cm tall, disease-free, and healthy Chinese hollyhock, and use pruning shears to make a flat cut 20 cm above the soil layer of the nutrient bag, keeping the cut smooth; S3: Selection of scion: Select the mother tree for scion, which is healthy, free of pests and diseases, and blooms luxuriantly; S4: Collection and treatment of scions: Grafting scions should be collected before the end of February. The collected branches should be soaked in carbendazim solution for sterilization and disinfection, and 2 / 3 of the leaves on the branches should be cut off to reduce water evaporation; when grafting, they should be cut into stem segments with 1-2 buds, and all leaves and petioles above the base of the petiole should be cut off to accelerate the complete and natural shedding of the petiole without damaging the axillary buds. In addition, in order to promote the differentiation of axillary buds, the top buds of the stem segments should be removed; S5: Preparation of wound healing promoting agent: 30mg / L6-BA+0.2mg / LKT+40mg / LNAA+200mg / L octahydroxyquinoline sulfate+sticky yam mucus (acting as a water-retaining agent) 1L; S6: scion and rootstock combined; The scion obtained in S4 is tightly attached to the inner side of the cut surface of the stock by cleft grafting, the wound healing promoting agent obtained in S5 is applied to the wound attachment area, and the entire scion and wound are tightly sealed with a plastic film; S7: After grafting, spread damp moss evenly on top of the soil layer in the nutrient bag to keep it moist.

2. The method for improving the survival rate of Yunnan Worry-Free Flower grafting according to claim 1, wherein The healing agent in S4 is generally required to be prepared and used immediately. When used, the healing agent is brushed on the grafting wound with a soft brush to form a water-retaining layer.

3. The method for improving the survival rate of Yunnan worry-free flower grafting according to claim 1, wherein The preparation method of the water-retaining agent in the wound healing promoting agent in S5 is: cut the cross section of the sticky yam tuber with a knife, scrape the mucus from the fresh section with a scraper, and store it in a brown light-proof bottle.

4. The method for improving the survival rate of Yunnan Worry-Free Flower grafting according to claim 1, wherein: The thickness of the plastic film in S6 is 0.06 mm.

5. The method for improving the survival rate of Yunnan Worry-Free Flower grafting according to claim 1, wherein The method of using the healing agent is as follows: Add 30mg / L6-BA+0.2mg / LKT+40mg / LNAA+200mg / L octahydroxyquinoline sulfate to the collected mucus. Use the concentration of the water retaining agent as indicated on the package and apply it to the wound.

6. A system for improving the survival rate of Yunnan Worry-Free Flower grafting, the system comprising: Tool preparation module, used to prepare tools such as pruning shears, grafting knives, plastic film, etc. The rootstock selection module is used to select a biennial, moderately tall, pest-free, and robust Chinese hollyhock, and cut an incision at a designated location; The scion selection module is used to select scions from excellent individual plants that are healthy, free of pests and diseases, and bloom luxuriantly; The scion processing module is used to sterilize and disinfect the collected scions, and perform leaf and stem cutting operations; A wound healing agent preparation module, used to prepare a wound healing agent with a specific ratio; The scion and stock combination module is used to fit the treated scion tightly to the inner side of the cut surface of the stock and bandage it with wound healing agent and plastic film; Moisturizing module for moisturizing with damp moss after grafting is completed.

7. The system of claim 6, wherein the healing agent treatment module further comprises: A ready-to-use unit to ensure immediate preparation and immediate use of the healing agent; The brushing unit is used for brushing the healing agent on the grafted wound joint to form a water-retaining layer.

8. The system according to claim 6, wherein the wound healing agent preparation module further comprises: The water retaining agent preparation unit is used for scraping the mucus from the fresh cross section of the sticky yam tuber and storing it in a brown light-proof bottle for use as a water retaining agent.

9. The system according to claim 6, wherein the plastic film used in the scion and stock combining module has a specific thickness of 0.06 mm to ensure good sealing and air permeability, thereby improving the survival rate of grafting.

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