Application of molybdenum-regulated synergistic substance chrysin in promoting crop growth and root development

Abstract

The invention provides an application of a molybdenum-regulated synergistic substance chrysin in promoting crop growth and root development, and systematically studies the influence of exogenous application of chrysin on soybean growth and on the form and content of phosphorus in soil. By comparing the difference between an experimental group and a control group, chrysin is revealed to promote the development of soybean root systems. Chrysin has positive influence on photosynthesis and physiological metabolism of soybeans, absorption and accumulation of phosphorus by the overground parts of the soybeans are remarkably improved, and the overall growth process of the soybeans is powerfully promoted.

Description

Technical Field

[0001] The invention relates to the field of agricultural planting, and in particular to the application of chrysin, a molybdenum-regulated synergistic substance, in promoting crop growth and root development. Background Art

[0002] As one of the most important grain and oil crops in the world, the growth and yield of soybean (Glycine max) are directly related to global food security and the stability of the agricultural economy. In China, soybean is not only a key raw material for the food and feed industry, but also a major source of high-quality plant protein and edible oil. However, in the face of growing market demand, domestic soybean production is far from self-sufficient and relies heavily on imports, which poses a challenge to national food security. Therefore, improving soybean yield and quality and optimizing planting technology have become important directions of current agricultural research.

[0003] The root development of soybeans plays a vital role in their growth process. The root system is the main organ for plants to absorb water and nutrients. The optimization of its morphology and structure is directly related to the overall growth potential, stress resistance and yield of the plant. Soybean plants with well-developed root systems can more effectively absorb nutrients from the soil, such as nitrogen, phosphorus, potassium, etc., thereby supporting the lush growth and high yield of the aboveground parts. Therefore, promoting the development of soybean root systems is one of the key strategies to increase soybean yields.

[0004] In recent years, the role of flavonoid compounds in plant growth and development has received increasing attention. As plant secondary metabolites, flavonoids are widely present in plants and have multiple biological functions. Among them, flavonoids show great potential in promoting plant root development. They can not only act as signal molecules to regulate the division and elongation of root cells, but also improve the soil microbial environment and promote the interaction between plants and soil, thereby indirectly promoting the growth and development of the root system.

[0005] Chrysin, as an important flavonoid compound, has been gradually explored in the agricultural field in recent years due to its unique chemical structure and biological activity. Studies have shown that chrysin has significant effects in regulating plant growth and development and improving stress resistance. In particular, in promoting plant root development, chrysin may optimize root configuration and improve the efficiency of root nutrient absorption by affecting the synthesis and signal transduction of plant hormones. In addition, chrysin may also indirectly promote the healthy growth of soybean roots by regulating the structure of soil microbial communities and improving the soil microenvironment, but the application of chrysin on soybean growth and phosphorus absorption has not yet been studied. Summary of the invention

[0006] In view of the above problems, the present invention provides an application of a molybdenum-regulated synergistic substance chrysin in promoting crop growth and root development, studies the effects of exogenous application of chrysin on soybean growth, as well as the form and content of phosphorus in the soil, and conducts in-depth research on the effects of chrysin on the soybean root system, aiming to optimize the root structure of soybeans, thereby improving their nutrient absorption efficiency and growth performance.

[0007] A molybdenum-regulated synergistic substance, phlorizin, is used in promoting crop growth and activating soil phosphorus. The synergistic substance contains chrysin, and the synergistic substance is used for the following purposes:

[0008] Increase plant height, aboveground dry weight, root length, root surface area, root volume, and root dry weight of crops;

[0009] Increase the net photosynthetic rate, intercellular CO2 concentration, and transpiration rate of crops;

[0010] Increase the phosphorus content and phosphorus accumulation in the aboveground parts of crops.

[0011] The crop is selected from soybean.

[0012] The synergistic substance is a solution containing chrysin, and the concentration of chrysin in the solution is 1-10 μmol / L.

[0013] Preferably, the concentration of chrysin in the solution is 10 μmol / L.

[0014] The solution contains phosphorus nutrients and molybdenum nutrients.

[0015] The molybdenum nutrient is (NH4)6Mo7O 24 ·4H2O.

[0016] The concentration of the molybdenum nutrient in the solution is 0.02 μM.

[0017] The present invention provides an application of chrysin in promoting soybean growth. At an optimal application concentration of 10 μmol / L, the development of soybean root system is significantly improved, including increase of root length, root surface area and root volume, thereby optimizing the photosynthesis efficiency and physiological metabolic capacity of soybean, improving the absorption and accumulation of phosphorus, and finally promoting the overall growth of soybean. The present invention also explores the potential of using chrysin as an efficient and environmentally friendly fertilizer additive to support high-yield and high-quality cultivation of soybean and other crops. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 To study the effect of molybdenum application on phosphorus absorption and translocation in soybean under different phosphorus levels.

[0019] Figure 2 The number of metabolites with significant changes mediated by Mo under Control treatment;

[0020] Figure 3 is the number of metabolites with significant changes mediated by Mo under P treatment;

[0021] Figure 4 The 50 differential metabolites mediated by Mo under Control treatment;

[0022] Figure 5 There are 94 different metabolites mediated by Mo under P treatment;

[0023] Figure 6 The changes of chrysin abundance under different phosphorus levels mediated by Mo application;

[0024] Figure 7 To study the effects of adding different chrysin concentrations on soybean growth phenotype;

[0025] Figure 8 To study the effects of adding different chrysin concentrations on soybean growth indicators;

[0026] Fig. 9 Effects of different chrysin concentrations on soybean phosphorus absorption. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solution and advantages of the present invention more clear, the present invention is further described in detail below in conjunction with specific implementation methods. It should be understood that the specific implementation methods described here are only used to explain the present invention and do not limit the scope of protection of the present invention.

[0028] Example 1: Effect of molybdenum application on soybean phosphorus absorption

[0029] The experiment set up two phosphorus addition levels: 0.015g / kg (to ensure normal growth of crops in the early stage), represented by Control; 0.15g / kg, represented by P. Two molybdenum addition levels: 0mg / kg, represented by -Mo; 0.15mg / kg, represented by +Mo. There were 4 treatments in total, namely Control-Mo, Control+Mo, P-Mo, and P+Mo. Each treatment was repeated 4 times, with a total of 16 pots. The soybean variety for the test was Tianlong No. 1. The soil was placed in the inner and outer chambers of the root box at the same time and compacted. The soybean seeds were sown in the inner chamber for growth. Each barrel, including the root box, was filled with 8.0kg of soil.

[0030] like Figure 1As shown in the figure, after comparative analysis, when the molybdenum addition level was the same, phosphorus treatment significantly increased the aboveground phosphorus content, phosphorus transport coefficient, aboveground phosphorus accumulation, root phosphorus accumulation and overall phosphorus accumulation of soybean, but had no significant effect on the root phosphorus content. On the other hand, when the phosphorus level was the same, compared with the non-molybdenum treatment, the molybdenum treatment not only significantly increased the aboveground phosphorus content, phosphorus transport coefficient and aboveground and whole plant phosphorus accumulation of soybean, but also significantly reduced the root phosphorus content and accumulation of soybean under the two phosphorus levels. This shows that molybdenum application can effectively improve the phosphorus absorption efficiency of soybean at different phosphorus levels, and promote the transport of root phosphorus to the aboveground part, thereby increasing the aboveground phosphorus accumulation and reducing the root phosphorus accumulation.

[0031] Example 2: Effect of molybdenum application on soybean rhizosphere metabolites

[0032] Since molybdenum application can significantly promote the increase of available phosphorus content in soil, metabolomics methods can be used to systematically screen substances whose metabolite abundance in soybean roots changes significantly under molybdenum application treatment.

[0033] Figure 2 to Figure 5 We identified 584 metabolites after exhaustive analysis in both positive and negative ion modes. Figure 2 and Figure 4 In the experiment, under the treatment condition of Control phosphorus addition level, the effect of molybdenum application on metabolites was significant. After molybdenum application, the abundance of 19 metabolites showed an upward trend, the abundance of 31 metabolites decreased, and the abundance of the remaining 534 metabolites did not change significantly. Figure 3 and Figure 5 Among the metabolites affected by molybdenum and phosphorus application under P treatment, the abundance of 33 metabolites increased significantly after molybdenum application, while the abundance of 61 metabolites decreased significantly. Further KEGG pathway enrichment analysis showed that under P+Mo treatment, the differential metabolites were mainly enriched in four key metabolic pathways, including flavonoid biosynthesis, phenylpropanoid biosynthesis, caprolactam degradation, and carbohydrate digestion and absorption.

[0034] Figure 6In the treatment condition with phosphorus addition level of P, the enrichment of differential metabolites was more extensive, and was significantly distributed in 20 metabolic pathways, especially ABC transporters, galactose metabolism, cysteine ​​and methionine metabolism, flavonoid biosynthesis, thermogenesis, carbohydrate digestion and absorption, mineral absorption, and EGFR tyrosine kinase inhibitor resistance. Metabolic pathways showed more significant enrichment. Given that the differential metabolites were significantly enriched in the flavonoid biosynthesis pathway under two different phosphorus levels, we conducted an in-depth analysis of the flavonoid substances that changed differentially under these two phosphorus levels. Under the P treatment, the application of molybdenum significantly increased the abundance of vigneron, phloridzin, neoastilbin, hesperidin 5-O-glucoside, and catechin 7-glucoside. Under the treatment of molybdenum application, the abundance of a flavonoid substance-chrysin was found to be significantly different after identification, such as Figure 6 Compared with other flavonoids, chrysin has the advantages of low cost and wide sources.

[0035] Example 3: Determination of Chrysin Concentration

[0036] Chrysin (expressed as Cs) was used to test the growth-promoting effect of soybean. The nutrient solution culture test site was the glass greenhouse of the College of Resources and Environment of Huazhong Agricultural University. The growth conditions were set at 25°C and 14 hours of light. The plastic box was used as the culture container. The specifications of the plastic box were 30.3×20.5×8.5cm. The plastic box and the foam board cover were opaque. Each pot contained 3L of nutrient solution. The nutrient solution formula was 4mM Ca(NO3)2·4H2O, 1mM NH4NO3, 1mM KH2PO4, 5mM KNO3, 2mM MgSO4·7H2O, 9.55μM MnSO4·4H2O, 46.28μM H3BO3, 0.77μM ZnSO4·7H2O, 0.32μM CuSO4·5H2O, 0.02μM (NH4)6Mo7O 24 4H2O, 98.09μM EDTA-Fe, the nutrient solution was changed every 4 days.

[0037] Chrysin was dissolved in 0.5mL 80% methanol and added to the nutrient solution. The chrysin concentration in the nutrient solution was set to 0μmol / L, 0.5μmol / L, 1μmol / L, 2μmol / L, 5μmol / L, and 10μmol / L. The same amount of 80% methanol was added to the control treatment, and each treatment was repeated 3 times. Soybeans were harvested after 30 days of growth, and the dry matter weight and phosphorus content of the plants were determined. Select soybean seeds with complete seed coat and full grains, soak them in 2% sodium hypochlorite solution for 15 minutes for disinfection, clean them with ultrapure water, soak them for 3 hours, so that the soybeans can fully absorb water and expose the embryos, and then evenly sow them in vermiculite for seedling cultivation. After 4 days, they grow to about 5cm in height and transplant them when the cotyledons are unfolded. Select soybean seedlings with consistent growth, plant 6 soybeans in each pot, and use sponges to clamp the soybeans to prevent them from sliding down. Soybeans are harvested after 30 days of growth.

[0038] Soybean phenotypic indicators and photosynthetic parameters were measured: (1) Plant height: The height of the main stem of soybean was measured with a tape measure at harvest time; (2) Dry weight: The samples of various parts of soybean were sterilized at 105°C for 30 minutes, and the dry weight was recorded after being dried at 60°C to constant weight; (3) Photosynthetic parameters: Net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate were measured using a portable photosynthetic meter (LICOR-6400XT) between 8 and 11 am on a clear day. (4) Determination of plant phosphorus content: Weigh the plant sample, put 0.1500g into a 50ml digestion tube, add 5ml of concentrated sulfuric acid, let it stand overnight, and then preheat it at 160°C in an infrared digestion furnace for 10-15 minutes, then raise the temperature to 280°C, take it out and cool it slightly, then add a small amount of 30% H2O2 and repeat several times until the digestion liquid becomes clear, and then use a flow analyzer to determine the phosphorus content after constant volume filtration.

[0039] After scanning the root system using a scanner Epson Scan System (V700PHOTO, Epson, Japan), the root length, root volume, root surface area, and root diameter were analyzed using the root imaging analysis software WinRHI-ZO Version 2009PRO (Quebec, Canada). The analysis results are shown in Table 1.

[0040] Table 1 Effects of chrysin on soybean root morphology

[0041]

[0042] From the experimental results shown in Table 1, it can be seen that under the treatment of different concentrations of chrysin, the root length and root volume of soybean showed a significant growth trend with the increase of treatment concentration. Specifically, when the treatment concentration reached 10μmol / L, the root length and root volume of soybean reached the maximum value. Compared with the control group, the root length increased significantly by 13%, and the root volume increased significantly by 10%. In addition, a similar growth trend was observed in the root surface area of ​​soybean, which increased by 19% compared with the control group. However, under various treatment concentrations of chrysin, the root diameter of soybean did not show significant changes. These experimental results further confirmed that chrysin has a positive promoting effect on soybean root growth.

[0043] Furthermore, chrysin not only promoted the development of soybean root system, but also this promotion effect further led to a significant increase in photosynthesis. Specifically, with the increase of chrysin treatment concentration, the root system of soybean was strengthened, which in turn caused its net photosynthetic rate and intercellular CO2 concentration to show a gradual upward trend. The impact results are shown in Table 2. At the treatment concentrations of chrysin 5 and chrysin 10, this improvement effect reached the maximum. In particular, under the treatment of 10μmol / L chrysin, due to the significant development of the root system, the net photosynthetic rate, intercellular chrysin concentration and transpiration rate of soybean achieved significant increases of 28.07%, 19.49% and 17.91%, respectively, while no significant changes in stomatal conductance due to chrysin treatment were observed. These results indicate that chrysin enhances the ability of soybean photosynthesis by promoting root development.

[0044] Table 2 Effect of chrysin on soybean photosynthetic rate

[0045]

[0046] like Figure 8 As shown in the figure, when the concentration of chrysin (Cs) was 10 μmol / L, the dry weight of soybean roots was significantly increased by 18.70%, indicating that chrysin has a significant promoting effect on the growth and development of soybean roots. Furthermore, good root development laid a solid foundation for the healthy growth of the aboveground part, which in turn led to a significant increase of 19.55% and 26.19% in plant height and aboveground dry weight, respectively.

[0047] like Fig. 9 As shown in the figure, chrysin (Cs) significantly promoted the phosphorus content and phosphorus accumulation in the aboveground part of soybean. Specifically, after applying different concentrations of chrysin, the phosphorus content and phosphorus accumulation in the aboveground part of soybean showed a trend of first increasing and then decreasing, and reached a peak under the treatment conditions of Cs1, Cs2 and Cs5. This trend indicates that chrysin optimizes the distribution of phosphorus to the aboveground part by promoting the development of the root system.

[0048] The present invention provides an application of chrysin in promoting soybean growth, systematically studies the effect of molybdenum application on chrysin in soybean root system, and applies chrysin on soybean growth, soybean root system and the form and content of phosphorus in soil through exogenous regulation of molybdenum application.

[0049] Please note that the above embodiments are only used to illustrate the present method and do not constitute a limitation on the scope of protection. Various equivalent replacements and modifications can be made to the above embodiments without departing from the spirit and essence of the present method. Although the present invention has been specifically described in conjunction with various specific preferred embodiments of the present invention, it should be understood that the present invention should not be limited to these specific embodiments. In fact, various modifications as described above that are obvious to those skilled in the art to obtain the invention should be included in the scope of the present invention.

Claims

1. An application of a molybdenum-regulated synergistic substance, chrysin, in promoting crop growth and root development, wherein the synergistic substance contains chrysin and is used for the following purposes: Increase plant height, aboveground dry weight, root length, root surface area, root volume, and root dry weight of crops; Increase the net photosynthetic rate, intercellular CO2 concentration, and transpiration rate of crops; Increase the phosphorus content and phosphorus accumulation in the aboveground parts of crops.

2. The use according to claim 1, characterized in that: The crop is selected from soybean.

3. The use according to claim 1, characterized in that: The synergistic substance is a solution containing chrysin, and the concentration of chrysin in the solution is 1-10 μmol / L.

4. The use according to claim 3, characterized in that: The concentration of chrysin in the solution is 10 μmol / L.

5. The use according to claim 3, characterized in that: The solution contains phosphorus nutrients and molybdenum nutrients.

6. The use according to claim 3, characterized in that: The molybdenum nutrient is (NH4)6Mo7O 24 ·4H2O.

7. The use according to claim 6, characterized in that: The concentration of the molybdenum nutrient in the solution is 0.02 μM.

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